CN107389549A - Rotating disc type collaurum/fluorescent test paper chip - Google Patents
Rotating disc type collaurum/fluorescent test paper chip Download PDFInfo
- Publication number
- CN107389549A CN107389549A CN201710706968.XA CN201710706968A CN107389549A CN 107389549 A CN107389549 A CN 107389549A CN 201710706968 A CN201710706968 A CN 201710706968A CN 107389549 A CN107389549 A CN 107389549A
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- Prior art keywords
- pond
- collaurum
- passage
- pool
- substrate
- Prior art date
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- 239000007788 liquid Substances 0.000 claims abstract description 45
- 239000000758 substrate Substances 0.000 claims abstract description 30
- 238000004140 cleaning Methods 0.000 claims abstract description 21
- 239000003550 marker Substances 0.000 claims abstract description 11
- 230000004888 barrier function Effects 0.000 claims abstract description 4
- 239000002699 waste material Substances 0.000 claims description 11
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 238000001215 fluorescent labelling Methods 0.000 claims 1
- 239000006210 lotion Substances 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 27
- 230000007774 longterm Effects 0.000 abstract description 3
- 238000012544 monitoring process Methods 0.000 abstract description 3
- 230000000737 periodic effect Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 6
- 239000012530 fluid Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 239000008293 association colloid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000003163 gonadal steroid hormone Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of rotating disc type collaurum/fluorescent test paper chip, including substrate；The substrate is provided with addition pool, is preset with the combination pond of collaurum and/or fluorescent marker, multiple colour developing ponds；The addition pool, with reference to pond and it is multiple colour developing pond be sequentially communicated by passage；The species sum of collaurum and fluorescent marker in the combination pond is corresponding with the quantity in pond of developing the color；The colour developing pond is the colour developing pond of collaurum or fluorescence reaction；The cleaning liquid pool that background colour interference is removed for being rinsed to colour developing pond is additionally provided with the substrate, the cleaning liquid pool is connected with passage, and junction is provided with the film that liquid barrier passes through.The chip volume of the present invention is small, and detection is simple to operate, as a result reads quick, and the patient especially suitable for needing periodic detection and long term monitoring is in self-test, eliminates patient and hurries back and forth in the trouble of hospital.
Description
Technical field
The present invention relates to technical field of biological, in particular to a kind of rotating disc type collaurum/fluorescent test paper chip.
Background technology
Current detection technique, substantially by the way of a kind of Test paper is directed to a kind of detection project, such as, detection
HCG uses special pregnant test paper.And if same sample to be detected when needing to detect multiple projects, it is necessary to by different examinations
Paper is detected, and detection efficiency is influenceed very big.For example, when needing to detect six, sex hormone, it is necessary to enter for six kinds of hormones
The different detections of six kinds of row, also make troubles while influenceing detection efficiency to person to be detected, spend the time longer, can not also meet
Family detection.
The content of the invention
It is an object of the invention to overcome existing Test paper can only a kind of project of single detection the problem of, there is provided it is a kind of
Rotating disc type collaurum/fluorescent test paper chip, it has the characteristics of spending less sample size to detect a variety of projects to be detected simultaneously.
To achieve the above object, rotating disc type collaurum/fluorescent test paper chip designed by the present invention, including substrate；It is described
Substrate is provided with addition pool, is preset with the combination pond of collaurum and/or fluorescent marker, multiple colour developing ponds；The addition pool, knot
Close pond and multiple colour developing ponds are sequentially communicated by passage；Collaurum and fluorescent marker in the combination pond species sum with
The quantity in colour developing pond is corresponding；The colour developing pond is the colour developing pond of collaurum or fluorescence reaction；It is additionally provided with and is used on the substrate
The cleaning liquid pool for removing background colour interference is rinsed to colour developing pond, the cleaning liquid pool is connected with passage, and junction is set
There is the film that liquid barrier passes through.
Further, the addition pool be one, the combination pond be one, the addition pool, with reference to pond and multiple colour developings
Pond is connected one by one successively by passage.
Further, the passage is twist laid in the substrate upper surface；The addition pool is located at the passage
It is inner；The base plate bottom center, which is provided with, to be used to coordinate with rotary drive to drive the groove that substrate level rotates.
Preferably, the passage is capillary channel.
Alternatively, it is additionally provided with waste liquid pool on the substrate；The end of waste liquid pool passage after colour developing pond.
Further, it is provided with blotting paper in the waste liquid pool.
Preferably, the addition pool and cleaning liquid pool are located in the same pit on substrate, and the film is along cross section
Direction is arranged in the pit is divided into two parts up and down by pit, and top half is the addition pool, and the latter half is institute
State cleaning liquid pool.
Preferably, the cleaning liquid pool is connected by a branched bottom with the passage, and the film is located at branched bottom
It is interior, and the film is that can be cleaned the film that liquid breaks through in liquid pool under the action of the centrifugal force.
The beneficial effects of the invention are as follows：
1st, by the effect of capillary channel, by the sample to be checked of addition pool by capillarity, it is transferred to corresponding combination
Pond, i.e. sample show with continuing to enter under capillary action after the corresponding collaurum with reference in pond and/or fluorescent marker reaction
Color pond, developed the color after reaction, and unnecessary sample enters waste liquid pool.Due to that can set multigroup detection line on one piece of substrate, and capillary is led to
Road consumption sample size is few, and sample movement is fast, therefore it may only be necessary to which the collaurum combined in pond and/or fluorescent marker are arranged into pin
To different detection projects, you can realize same sample while detect a variety of different detection projects, substantially increase detection efficiency,
Detected suitable for family.
2nd, it is provided with by passage and base plate bottom and below addition pool and is used to coordinate to drive base with rotary drive
The effect of the groove of plate level rotation, while substrate is rotated, the sample to be checked of addition pool is existed under the influence of centrifugal force
Quickly moved in passage.Due to the effect of centrifugal force, the sample consumption in passage is not only few, and sample movement is fast, chip volume
It is small, same sample can be achieved and detect a variety of different detection projects simultaneously.
3rd, by cleaning the effect of liquid pool and film, after sample-adding, sample arrives first is reacted with reference to pond, then to colour developing pond
Colour developing；Rotary plate, by centrifugal action, the cleaning fluid cleaned in liquid pool is carried out clearly after breaking through film to passage and colour developing pond
Wash, effectively eliminate background colour interference.
Brief description of the drawings
Fig. 1 is a kind of structural representation of embodiment of rotating disc type collaurum/fluorescent test paper chip of the present invention.
Fig. 2 is A-A faces schematic cross-sectional view in Fig. 1.
Fig. 3 is Fig. 1 upward view.
Fig. 4 is the schematic diagram that Fig. 2 is provided with after rotary drive.
Fig. 5 is the dimensional structure diagram of rotary drive in Fig. 4.
Fig. 6 is the structural representation of rotating disc type collaurum of the present invention/fluorescent test paper chip another embodiment.
Fig. 7 is Fig. 6 close-up schematic view.
In figure, 1. substrates；2. addition pool；3. combine pond；4. passage；5. pond of developing the color；6. waste liquid pool；7. groove；8. rotation
Actuator；9. clean liquid pool；10. film；11. branched bottom.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail.
Embodiment 1
Rotating disc type collaurum/fluorescent test paper chip as shown in Fig. 1~5, including substrate 1；Substrate 1 be provided with addition pool 2,
It is preset with the combination pond 3 of collaurum and/or fluorescent marker, multiple colour developing ponds 5；Addition pool 2, with reference to pond 3 and it is multiple colour developing pond 5
It is sequentially communicated by passage 4；It is relative with the quantity in pond 5 of developing the color with reference to the species of the collaurum in pond 3 and fluorescent marker sum
Should；Pond 5 develop the color for collaurum or the colour developing pond 5 of fluorescence reaction；It is additionally provided with substrate 1 for being rinsed removal to colour developing pond 3
The cleaning liquid pool 9 of background colour interference, cleaning liquid pool 9 is connected with passage 4, and junction is provided with the film that liquid barrier passes through
10。
Addition pool 2 is one, with reference to pond 3 is one, addition pool 2, with reference to pond 3 and multiple colour developing ponds 5 by passage 4 successively
Connect one by one.
Passage 4 is preferably capillary channel, and passage 4 is twist laid in the upper surface of substrate 1.Waste liquid is additionally provided with substrate 1
Pond 6；The end of the passage 4 after colour developing pond 5 of waste liquid pool 6.Blotting paper (not shown) is also provided with waste liquid pool 6.
Addition pool 2 is located at the inner of passage 4；The bottom centre of substrate 1, which is provided with, to be used to rotary drive 8 coordinate to drive base
The groove 7 that plate 1 rotates horizontally.
Preferably, groove 7 is coaxially disposed with addition pool 2.Detection is applied mechanically in matching somebody with somebody for rotating disc type collaurum/fluorescent test paper chip
Device is provided with the rotary drive 8 coordinated with groove 7.Detection means can be in corresponding rotating disc type collaurum/fluorescent test paper chip
The opening position of addition pool 2, the breach for liquid feeding is left, for sample or cleaning fluid is added dropwise to chip.
Because the bottom of substrate 1 is provided with the groove 7 coordinated with rotary drive 8, by the effect of rotary drive 8, can drive
Dynamic substrate 1 rotates, and, accelerates its translational speed, realizes quick detection so as to sample under the influence of centrifugal force.
Addition pool 2 and cleaning liquid pool 9 are located in the same pit on substrate 1, and film 10 is arranged in along cross-sectional direction
Pit is divided into two parts up and down in pit, top half is addition pool 2, and the latter half is cleaning liquid pool 9.
By cleaning the effect of liquid pool 9 and film 10, after sample-adding, sample arrives first is reacted with reference to pond, then to colour developing pond
5 colour developings；Film 10 is punctured by pricking object, rotary plate 1, or by larger centrifugal action, the cleaning fluid cleaned in liquid pool 9
Passage 4 and colour developing pond 5 are cleaned after breaking through film 10, effectively eliminate background colour interference.
The sample of the present invention can be blood, urine or other body fluid or liquid for containing determinand.
Multiple collaurums and/or fluoroscopic examination can be integrated on a chip by the present invention, realize multi-channel reaction, soon
It is fast, sensitive, if chip use is collaurum detection mode, then result can use naked-eye observation, or instrument point
Reading is distinguished, needs corresponding fluorescence detection equipment to carry out reading if the detection comprising fluorescence reaction, above-mentioned reading instruments are
Prior art, or simply transform what can be obtained based on existing instrument, it will not go into details in the present invention.
The chip volume of the present invention is small, and detection is simple to operate, as a result read it is quick, especially suitable for need periodic detection and
The patient of long term monitoring is in self-test, eliminates patient and hurries back and forth in the trouble of hospital.
Embodiment 2
As shown in Figure 6,7, the technical scheme of the rotating disc type collaurum/fluorescent test paper chip of the present embodiment and the base of embodiment 1
This is identical, and difference is that clean liquid pool 9 is connected by a branched bottom 11 with passage 4, and film 10 is located in branched bottom 11, and
Film 10 is that can be cleaned the film that liquid breaks through in liquid pool 9 under the action of the centrifugal force.Sample arrives first after sample-adding enters with reference to pond 3
Row reaction, then developed the color to colour developing pond 5；Rotary plate 1, by centrifugal action, the cleaning fluid cleaned in liquid pool 9 breaks through film 10
Passage 4 and colour developing pond 5 are cleaned afterwards, effectively eliminate background colour interference.
Above-described embodiment can be arranged as by the addition pool 2 being sequentially communicated by passage 4, with reference to pond 3, multiple colour developing ponds 5
One group of detection line, laid with addition pool 2 for multigroup detection line of starting point in multi-spiral shape, so as to be integrated more in a chip
More different detection projects.
The material that SMIS sheet of the present invention preferably uses be can with association colloid gold or fluorescent marker material, such as
Material used by Elisa plates, it is commercially available to buy.Therefore, all it is incorporated in core with reference to the collaurum in pond 3 or fluorescent marker
In sheet, property can stably preserve for a long time, and practicality is good.
The chip volume of the present invention is small, and detection is simple to operate, as a result read it is quick, especially suitable for need periodic detection and
The patient of long term monitoring is in self-test, eliminates patient and hurries back and forth in the trouble of hospital.
Claims (8)
- A kind of 1. rotating disc type collaurum/fluorescent test paper chip, it is characterised in that：Including substrate (1)；The substrate (1) is provided with Addition pool (2), the combination pond (3) for being preset with collaurum and/or fluorescent marker, multiple colour developings pond (5)；The addition pool (2), It is sequentially communicated with reference to pond (3) and multiple colour developings pond (5) by passage (4)；Collaurum and fluorescence labeling in the combination pond (3) The species sum of thing is corresponding with the quantity of colour developing pond (5)；Colour developing pond (5) is the colour developing pond of collaurum or fluorescence reaction (5)；The cleaning liquid pool (9) that background colour interference is removed for being rinsed to colour developing pond (5), institute are additionally provided with the substrate (1) State cleaning liquid pool (9) with passage (4) to be connected, and junction is provided with the film (10) that liquid barrier passes through.
- 2. rotating disc type collaurum/fluorescent test paper chip according to claim 1, it is characterised in that：The addition pool (2) is One, the combination pond (3) be one, the addition pool (2), with reference to pond (3) and it is multiple develop the color pond (5) by passage (4) according to It is secondary to connect one by one.
- 3. rotating disc type collaurum/fluorescent test paper chip according to claim 1, it is characterised in that：The passage (4) is in spiral shell Rotation shape is laid in the substrate (1) upper surface；The addition pool (2) is located at the inner of the passage (4)；Substrate (1) bottom Portion center, which is provided with, to be used to coordinate with rotary drive (8) to drive the groove (7) that substrate (1) rotates horizontally.
- 4. rotating disc type collaurum/fluorescent test paper chip according to claim 1, it is characterised in that：The passage (4) is hair Thin passage (4).
- 5. rotating disc type collaurum/fluorescent test paper chip according to claim 1, it is characterised in that：On the substrate (1) also Provided with waste liquid pool (6)；The end of the waste liquid pool (6) passage (4) after colour developing pond (5).
- 6. rotating disc type collaurum/fluorescent test paper chip according to claim 5, it is characterised in that：In the waste liquid pool (6) Provided with blotting paper.
- 7. rotating disc type collaurum/fluorescent test paper chip according to any one of claim 1~6, it is characterised in that：It is described to add Sample pond (2) and cleaning liquid pool (9) are located in the same pit on substrate (1), and the film (10) is arranged along cross-sectional direction In pit is divided into two parts up and down in the pit, top half is the addition pool (2), and the latter half is the cleaning Liquid pool (9).
- 8. rotating disc type collaurum/fluorescent test paper chip according to any one of claim 1~6, it is characterised in that：It is described clear Washing lotion pond (9) is connected by a branched bottom (11) with the passage (4), and the film (10) is interior located at branched bottom (11), And the film (10) is that can be cleaned the film that liquid pool (9) interior liquid breaks through under the action of the centrifugal force.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710706968.XA CN107389549A (en) | 2017-08-17 | 2017-08-17 | Rotating disc type collaurum/fluorescent test paper chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710706968.XA CN107389549A (en) | 2017-08-17 | 2017-08-17 | Rotating disc type collaurum/fluorescent test paper chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107389549A true CN107389549A (en) | 2017-11-24 |
Family
ID=60353268
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710706968.XA Pending CN107389549A (en) | 2017-08-17 | 2017-08-17 | Rotating disc type collaurum/fluorescent test paper chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107389549A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109187945A (en) * | 2018-09-29 | 2019-01-11 | 厦门大学 | A kind of laying method of colloid gold test paper on CD |
| CN112858291A (en) * | 2021-01-11 | 2021-05-28 | 新疆维吾尔自治区产品质量监督检验研究院 | Multi-parameter colloid diamond carbon food safety analyzer |
| CN113295686A (en) * | 2021-05-24 | 2021-08-24 | 上海市口腔病防治院 | Saliva sample pH detection method and special detection device thereof |
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| CN207147952U (en) * | 2017-08-17 | 2018-03-27 | 武汉璟泓万方堂医药科技股份有限公司 | Rotating disc type collaurum/fluorescent test paper chip |
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2017
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112858291A (en) * | 2021-01-11 | 2021-05-28 | 新疆维吾尔自治区产品质量监督检验研究院 | Multi-parameter colloid diamond carbon food safety analyzer |
| CN113295686A (en) * | 2021-05-24 | 2021-08-24 | 上海市口腔病防治院 | Saliva sample pH detection method and special detection device thereof |
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Address after: 430000 No.18, East biopark Road, Donghu New Technology Development Zone, Wuhan City, Hubei Province Applicant after: Wuhan Jinghong Technology Co., Ltd Address before: 430079 Hubei Jiangxia Wuhan District high tech Avenue No. 858 Optics Valley biological City pharmaceutical Park industrial base two phase A86-2 Applicant before: Wuhan Jinghong Wanfangtang Medical Technology Co., Ltd. |