CN107343926B

CN107343926B - Traditional Chinese medicine composition for treating recurrent oral ulcer

Info

Publication number: CN107343926B
Application number: CN201710721232.XA
Authority: CN
Inventors: 唐志立; 张燕; 黄梅; 彭贤东; 李林; 胡晓燕; 王娅俐
Original assignee: Nanchong Central Hospital
Current assignee: Nanchong Central Hospital
Priority date: 2017-08-22
Filing date: 2017-08-22
Publication date: 2020-05-29
Anticipated expiration: 2037-08-22
Also published as: CN107343926A

Abstract

The invention discloses a traditional Chinese medicine composition for treating recurrent oral ulcer, which is characterized by comprising the following components in parts by weight: is prepared from the following Chinese medicinal raw materials and water according to the mass ratio, and comprises 140-160 parts of prepared rhizome of rehmannia, 95-110 parts of rhizoma anemarrhenae, 90-110 parts of phellodendron, 85-95 parts of figwort, 45-55 parts of tuckahoe, 25-35 parts of schisandra chinensis, 90-110 parts of morinda officinalis, 55-65 parts of rhizoma bletillae and 45-55 parts of medicinal cyathula root. The invention treats both principal and secondary aspects of diseases, can treat both principal and secondary aspects of diseases, and considers the characteristics of diseases and the constitutional features of patients. Can improve the physique of the patient, enhance the immunity of the patient and avoid relapse while treating. The invention has the advantages of definite curative effect, safety, reliability, convenient taking, low price, easy acceptance by patients, simple preparation process and easy production.

Description

Traditional Chinese medicine composition for treating recurrent oral ulcer

Technical Field

The invention relates to a pharmaceutical composition for treating oral diseases, in particular to a traditional Chinese medicine composition for treating recurrent oral ulcer.

Background

The recurrent oral ulcer refers to the recurrent purulent or non-purulent painful ulcer of the mucous membrane of the oral cavity of a human, including the gingiva, the tongue body, the inner lip, the soft palate and the like. The frequency of the ulcer attack varies from person to person, and the ulcer attack frequently occurs many times every year, every other month or every month, and the severe one is fulminating, the ulcer attack does not occur intermittently, and the ulcer aggravates year by year, is not healed all the year round, and even accompanies all the year round. The disease generally does not endanger life, but the pain is great, which affects study, work and life. The western medicine considers the disease as seven major causes, such as psychological factors, mental factors and other factors. However, the specific pathogenesis is still unclear, so that no specific medicine is available in Western medicine for treating the disease at present. The traditional Chinese medicine considers that the occurrence reasons of the disease comprise external factors and internal factors, the internal factors are responsible for heat collection or yin deficiency, and the external factors are responsible for external pathogenic factors. The diseases are usually caused by wind-heat affecting the spleen, heat accumulation in the heart and spleen, and fire hyperactivity due to yin deficiency attacking the oral cavity. The spleen opens into the mouth and tongue as the seedling of heart, and the kidney connects with the tongue origin, connects with the throat and stomach meridians and collaterals and gums, so the lesion of the disease is caused by the heart, spleen, stomach and kidney, and the key is the burning of the oral cavity by fire pathogen, which is the type of deficient fire flaming. At present, the traditional Chinese medicine treatment mainly comprises the treatment of recurrent aphtha by oral administration of Chinese proprietary medicine bezoar antitoxic pill, and the green tea powder has certain effect on the treatment of recurrent aphtha. But has the problems of temporary solution, permanent solution, long treatment time and discomfort of a considerable part of people.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide the traditional Chinese medicine composition for treating recurrent oral ulcer, which can treat both root causes and symptoms. The object of the invention is solved by the following technical solutions; a traditional Chinese medicine composition for treating recurrent oral ulcer is prepared from the following traditional Chinese medicine raw materials and water according to the mass ratio: 140-160 parts of prepared rhizome of rehmannia, 95-110 parts of rhizoma anemarrhenae, 90-110 parts of golden cypress, 85-95 parts of radix scrophulariae, 45-55 parts of poria cocos, 25-35 parts of schisandra chinensis, 90-110 parts of morinda officinalis, 55-65 parts of rhizoma bletillae and 45-55 parts of radix cyathulae.

If necessary, 3 parts by mass of sodium benzoate and 0.3 part by mass of ethylparaben may be added.

A method for preparing a Chinese medicinal composition for treating recurrent oral ulcer comprises: soaking the nine decoction pieces in 8-10 times of water for 30 minutes, decocting for 2 times, each time for 2 hours, mixing the decoctions, filtering, concentrating the filtrate to the weight of the raw material medicines, standing for 12 hours, filtering, adding 3 parts by weight of sodium benzoate and 0.3 part by weight of ethylparaben into the filtrate, stirring to dissolve, adding 1.3 +/-0.1 times of purified water, stirring uniformly, and subpackaging to obtain the mixture.

Certainly, the invention can also be prepared into pills or tablets by the traditional Chinese medicine method, and the specific method is to further concentrate the concentrated solution into paste on the basis of the liquid preparation process, add sugar powder or starch and prepare granules or tablets.

The invention takes the prepared rehmannia root as a monarch drug, the prepared rehmannia root can greatly tonify kidney water, the kidney is a residence with water and fire, and deficiency fire cannot be generated when the kidney water is sufficient. The golden cypress, the rhizoma anemarrhenae and the radix scrophulariae are ministerial medicines, the golden cypress and the rhizoma anemarrhenae are matched to clear and purge deficiency fire and benefit generation of kidney yin, and the radix scrophulariae can clear and purge fire and detoxify deficiency fire caused by deficiency of kidney water, nourish kidney yin, enhance the nourishing effect of the radix rehmanniae preparata, and cause deficiency of kidney yin, deficiency fire and oral cavity ulceration. The schisandra chinensis can nourish lung yin, astringe lung metal, kidney metal, lung metal, metal water, child diseases and mother diseases, and when the lung yin is sufficient, the generation of the kidney yin can be facilitated; the radix morindae officinalis is warm in nature but not dry, and can induce fire to return to origin without damaging kidney yin; the poria cocos can promote kidney turbidity, remove spleen dampness, and remove new yin without generation if turbid yin is not removed, and meanwhile, the spleen dampness is removed, so that the greasy property of the prepared rehmannia root is reduced; the rhizoma bletillae, cold and cool with bitter, can dissipate blood stasis, has astringent taste and sticky texture, can reduce swelling and promote granulation, and can promote healing of ulcer. Therefore, the coordination of the schisandra, morinda officinalis, tuckahoe and rhizoma bletillae is beneficial to the tranquilization of water and fire in the kidney and the economy of water and fire, and the schisandra, morinda officinalis, tuckahoe and rhizoma bletillae are used as adjuvant drugs. Cyathula root, radix Cyathulae serves as a guiding drug for guiding drugs into the kidney meridian and directing heat downward. Compared with the prior art, the invention has the following advantages:

1. the invention treats both principal and secondary aspects of diseases, can treat both principal and secondary aspects of diseases, and considers the characteristics of diseases and the constitutional features of patients. Can improve the physique of the patient, enhance the immunity of the patient and avoid relapse while treating.

2. The bletilla striata for promoting granulation is added while nourishing yin, so that the repair of the ulcer surface can be accelerated, and the quick rehabilitation of diseases is facilitated.

3. The invention uses morinda officinalis to induce fire to return to original, has the characteristic of no damage to yin when the fire is induced to return to original, and can improve the effect and reduce the side effect.

4. The invention combines cold and heat, can tonify and purge simultaneously, has mild nature and taste, and can be taken for a long time.

5. The invention has the advantages of definite curative effect, safety, reliability, convenient taking, low price, easy acceptance by patients, simple preparation process and easy production.

The using method comprises the following steps: the mixture is taken three times a day, 30-40ml each time, and the pill and tablet are taken three times a day, each time is about 22-26 g of the original medicine.

Through various experiments, the invention has exact curative effect on treating recurrent oral ulcer and small toxicity, and the specific experiments and results are as follows:

1. it is known that the effect of the mixture of yellow ulcer on the swelling of the mouse feet caused by carrageenan

52 male mice with the weight of 18-22 g were selected and randomly divided into 5 groups. Respectively is a model group, a prednisone acetate group of 10mg/kg, a Zhihuang ulcer mixture of 5g, 10g and 20 g. The model group is given with 0.5% CMC-Na solution with the same volume, the other groups are respectively infused with corresponding drugs for stomach, 2 times a day, 3 days continuously, 30min after the last administration, each mouse is injected with 0.05ml of 1% carrageenan subcutaneously on the left hind foot, the mouse is killed by dislocation after 4h, the left hind foot and the right hind foot are symmetrically cut off from the ankle joint, the weight difference of the left and the right feet is taken as the swelling degree, and the results are statistically analyzed. The results are shown in Table 1.

Comparison with model groups: p <0.01, the same applies below.

As can be seen from Table 1, 10g of the crude drug/kg of the mixture for treating the yellow ulcer has a remarkable inhibitory effect on the swelling of the feet of the mice caused by the carrageenan.

2. Effect of the mixture of yellow ulcer on canker sore of rat

Rat models of canker sores were prepared according to the references in conjunction with the laboratory protocol. The method comprises the following specific operation steps: rats were anesthetized by intraperitoneal injection with chloral hydrate (350 mg/kg), and then injected with 0.05 ml/mouse of 20% acetic acid under the mucosa of the right buccal area of the mouth, and normal control group was injected with the same amount of normal saline, and the rats developed canker sores after 24 h. The next day (after 24 h) of molding, except for the normal control group, the 60 successfully molded rats were randomly divided into 5 groups, namely a model control group, a positive control group (200 mg/kg Guilin watermelon frost) and a known yellow ulcer mixture high-medium/low-dose group (16, 8 and 4g of crude drug/kg). The Zhihuangyangqi mixture is administrated by gastric lavage (about 0.5ml of liquid medicine is remained to be slowly injected into the oral cavity of a rat so as to ensure that the liquid medicine is fully contacted with the ulcer surface); directly taking the powder of Guilin watermelon crystal, uniformly coating the powder (1/4 of the dosage of a rat) on the ulcer surface when in administration, preparing the rest powder (3/4 of the dosage of the rat) into a suspension with 0.5 percent CMC-Na solution, and then irrigating the rat with stomach. Respectively intragastrically irrigating equivalent 0.5% CMC-Na solution for the model control group and the normal control group 2 times per day for 5 days continuously, killing the animals 1h after the last administration, and visually observing and grading the ulcer surface healing condition; fixing oral mucosa at ulcer with formaldehyde, and performing HE staining observation.

And (3) observing the rating index by naked eyes: the healing condition of the ulcer surface is graded as 0 grade, the ulcer is recovered to a normal state by visual observation, the ulcer surface has no obvious pseudomembrane on the I grade, the ulcer surface has a thin layer of yellow-white pseudomembrane, no edema exists around the ulcer surface on the II grade, and the ulcer surface has thicker pseudomembrane and inflammatory edema exists around the ulcer surface on the III grade. The results are shown in Table 2.

Comparison with model groups: p <0.01

By visually evaluating the ulcer healing grade of each group of animals, the positive group of watermelon frost and the Zhihuang ulcer mixture 8g of crude drug/kg can obviously improve the ulcer healing degree, and has obvious difference (P is less than 0.01) compared with a model group. The recovery condition of the yellow ulcer mixture of 16g crude drug/kg oral mucosa ulcer is improved to a certain extent compared with a model group, but the difference has no statistical significance.

Histopathological scoring: the histological grading standard of the oral ulcer is referred to relevant documents, and the actual condition of the experiment is combined to draw a diagnosis standard which is shown in a table 3. The histological grading results of the ulcer part of oral mucosa of each group of rats are shown in table 4.

Comparison with model groups: p < 0.05.

3. Toxicity test

The result of the preliminary test shows that half of Lethal Dose (LD) of the mixture for treating the gastric ulcer of the mice cannot be measured by the mixture for treating the gastric ulcer₅₀). Therefore, the maximum tolerance of the mixture for treating the ulcer: 40 mice with the weight of 18 g-22 g are divided into a control group and a known yellow ulcer mixture group randomly in half of male and female. After animals are fasted for 16 hours without water, respectively gavage the Zhihuang ulcer mixture with the maximum volume (0.4 ml/10 g) and the maximum concentration (1.5 g/kg)Or equal volume of distilled water. The administration was performed 2 times in 24 hours, once every 6 hours, the response of the mice was observed and recorded within 14 days after the administration, the animals were sacrificed at the end of the experiment, the heart, liver, spleen, lung and kidney were weighed for wet weight, and the organ index was calculated, and the results are shown in tables 5, 6 and 7.

As can be seen in Table 5, the mice given the known mixture of yellow ulcers exhibited signs of loose stools, which recovered after about 5 hours, but the animals did not die.

P <0.01 compared to control, the same below.

As can be seen from table 6, the weight gain of female mice after one week of administration of the yellow ulcer mixture was significantly slowed (P < 0.01), which is statistically different from that of the control group; after two weeks of administration, the normal condition was recovered, and the control group had no statistical difference.

As can be seen from Table 7, the index of each organ of the mice with the mixture of yellow ulcers is not statistically different from that of the control group. The result shows that the mouse orally administered known yellow ulcer mixture has no obvious acute toxicity, and the measured maximum tolerance of the mouse orally administered known yellow ulcer mixture is 120g of crude drug/kg, which is equivalent to 109.1 times of the clinical human dose.

Detailed Description

Example 1: a traditional Chinese medicine composition for treating recurrent oral ulcer is prepared from the following traditional Chinese medicine raw materials and water according to the mass ratio: 150g of prepared rhizome of rehmannia, 100g of rhizoma anemarrhenae, 110g of phellodendron, 90g of radix scrophulariae, 50g of tuckahoe, 35g of schisandra chinensis, 100g of morinda officinalis, 60g of rhizoma bletillae, 50g of radix cyathulae, 3g of sodium benzoate and 0.3g of ethylparaben.

A method for preparing a Chinese medicinal composition for treating recurrent oral ulcer comprises: adding the nine decoction pieces, adding 8-10 times of water, soaking for 30 minutes, decocting for 2 times, each time for 2 hours, mixing decoctions, filtering, concentrating the filtrate to the weight of the raw material medicines, standing for 12 hours, filtering, adding 3 parts by weight of sodium benzoate and 0.3 part by weight of ethylparaben into the filtrate, stirring to dissolve, adding purified water to 1.3 times of 0.1 time, stirring, and subpackaging to obtain the mixture.

Example 2: a traditional Chinese medicine composition for treating recurrent oral ulcer is prepared from the following traditional Chinese medicine raw materials and water according to the mass ratio: 140g of prepared rehmannia root, 95g of rhizoma anemarrhenae, 90g of golden cypress, 95g of radix scrophulariae, 45g of poria cocos, 25g of schisandra chinensis, 110g of morinda officinalis, 65g of rhizoma bletillae and 45g of radix cyathulae. The production method comprises the following steps: adding the above nine decoction pieces, soaking in 8-10 times of water for 30min, decocting for 2 times, each time for 2 hr, mixing decoctions, filtering, concentrating the filtrate to the mass of the raw materials, standing for 12 hr, filtering, concentrating the filtrate to thick paste, adding excipient sugar powder or starch, stirring, making into pill, and packaging.

Example 3: a traditional Chinese medicine composition for treating recurrent oral ulcer is prepared from the following traditional Chinese medicine raw materials and water according to the mass ratio: 160g of prepared rhizome of rehmannia, 110g of rhizoma anemarrhenae, 105g of phellodendron, 85g of radix scrophulariae, 55g of poria cocos, 30g of schisandra chinensis, 90g of morinda officinalis, 55g of rhizoma bletillae and 55g of radix cyathulae. The production method comprises the following steps: adding the above nine decoction pieces, soaking in 8-10 times of water for 30min, decocting for 2 times, each time for 2 hr, mixing decoctions, filtering, concentrating the filtrate to the mass of the raw materials, standing for 12 hr, filtering, concentrating the filtrate to thick paste, adding excipient sugar powder or starch, stirring, tabletting, and packaging.

Example 4: a traditional Chinese medicine composition for treating recurrent oral ulcer is prepared from the following traditional Chinese medicine raw materials and water according to the mass ratio: 140g of prepared rehmannia root, 110g of rhizoma anemarrhenae, 105g of golden cypress, 95g of radix scrophulariae, 55g of poria cocos, 25g of schisandra chinensis, 105g of morinda officinalis, 55g of rhizoma bletillae and 45g of radix cyathulae. The production method comprises the following steps: adding the nine decoction pieces, adding 8-10 times of water, soaking for 30 minutes, decocting for 2 times, each time for 2 hours, mixing decoctions, filtering, concentrating the filtrate to the mass of the raw material medicines, standing for 12 hours, filtering, adding 3 parts by mass of sodium benzoate and 0.3 part by mass of ethylparaben into the filtrate, stirring to dissolve, adding purified water to 1.3 times of 0.1 time, stirring, and subpackaging to obtain the mixture.

Example 5: a traditional Chinese medicine composition for treating recurrent oral ulcer is prepared from the following traditional Chinese medicine raw materials and water according to the mass ratio: prepared rehmannia root 150g, anemarrhena rhizome 100g, phellodendron bark 100g, scrophularia root 90g, poria cocos wolf 50g, schisandra fruit 30g, morinda root 100g, bletilla tuber 60g and cyathula root 50 g. The production method comprises the following steps: adding the nine decoction pieces, adding 8-10 times of water, soaking for 30 minutes, decocting for 2 times, each time for 2 hours, mixing decoctions, filtering, concentrating the filtrate to the mass of the raw material medicines, standing for 12 hours, filtering, adding 3 parts by mass of sodium benzoate and 0.3 part by mass of ethylparaben into the filtrate, stirring to dissolve, adding purified water to 1.3 times of 0.1 time, stirring, and subpackaging to obtain the mixture.

Claims

1. A traditional Chinese medicine composition for treating recurrent oral ulceration is characterized in that: is prepared from the following Chinese medicinal raw materials and water according to the mass ratio, and comprises 140-160 parts of prepared rhizome of rehmannia, 95-110 parts of rhizoma anemarrhenae, 90-110 parts of phellodendron, 85-95 parts of figwort, 45-55 parts of tuckahoe, 25-35 parts of schisandra chinensis, 90-110 parts of morinda officinalis, 55-65 parts of rhizoma bletillae and 45-55 parts of medicinal cyathula root.

2. The traditional Chinese medicine composition for treating recurrent oral ulceration as claimed in claim 1, wherein: is prepared from the following Chinese medicinal raw materials and water according to the mass ratio, wherein the Chinese medicinal raw materials comprise prepared rhizome of rehmannia 150, rhizoma anemarrhenae 100, phellodendron 100, radix scrophulariae 90, tuckahoe 50, schisandra chinensis 30, morinda officinalis 100, rhizoma bletillae 60 and radix cyathulae 50.

3. A traditional Chinese medicine composition for treating recurrent oral ulceration is characterized in that: is prepared from the following Chinese medicinal raw materials, 3 parts by weight of sodium benzoate, 0.3 part by weight of ethylparaben and water, 140-160 parts by weight of prepared rhizome of rehmannia, 95-110 parts by weight of rhizoma anemarrhenae, 90-110 parts by weight of golden cypress, 85-95 parts by weight of radix scrophulariae, 45-55 parts by weight of poria cocos, 25-35 parts by weight of schisandra chinensis, 90-110 parts by weight of morinda officinalis, 55-65 parts by weight of rhizoma bletillae and 45-55 parts by weight of radix cy.

4. A method for preparing the Chinese medicinal composition for treating recurrent oral ulcer of claim 3 comprises the following steps: the method is characterized in that: the preparation method comprises the following steps of adding 8-10 times of water into nine decoction pieces, soaking for 30 minutes, decocting for 2 times, each time for 2 hours, combining decoction liquids, filtering, concentrating the filtrate to the weight of the raw material medicines, standing for 12 hours, filtering, adding 3 parts by mass of sodium benzoate and 0.3 part by mass of ethyl hydroxybenzoate into the filtrate, stirring to dissolve, adding 1.3 +/-0.1 times of purified water, stirring uniformly, and subpackaging to obtain the mixture.