CN107299398A - A kind of microbiological degumming method of corn wrapper fibre - Google Patents
A kind of microbiological degumming method of corn wrapper fibre Download PDFInfo
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- CN107299398A CN107299398A CN201611187045.XA CN201611187045A CN107299398A CN 107299398 A CN107299398 A CN 107299398A CN 201611187045 A CN201611187045 A CN 201611187045A CN 107299398 A CN107299398 A CN 107299398A
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- aspergillus niger
- degumming
- wrapper fibre
- corn wrapper
- corn
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
- D01C1/00—Treatment of vegetable material
- D01C1/02—Treatment of vegetable material by chemical methods to obtain bast fibres
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
- D01C1/00—Treatment of vegetable material
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
- D01C1/00—Treatment of vegetable material
- D01C1/04—Bacteriological retting
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- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Textile Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
- Paper (AREA)
Abstract
The present invention provides a kind of microbiological degumming method of corn wrapper fibre, it is characterised in that described method comprises the following steps：（1）Select without the maize peel gone mouldy；（2）Dipping pretreatment is carried out to corn wrapper fibre；（3）Aspergillus niger（Aspergillus niger）HYA4 solid fermentations, prepare corn wrapper fibre microbial degumming liquid；（4）The microbial degumming of corn wrapper fibre；（5）Potassium hydroxide solution soaks；（6）Washing and drying obtains corn wrapper fibre.The present invention damages small to corn wrapper fibre, and the corn wrapper fibre residual gum content of preparation is relatively low, and intensity is higher, fiber softening.
Description
Technical field
The present invention relates to a kind of preparation technology recycled to agricultural waste material, and in particular to a kind of corn wrapper fibre
Microbiological degumming method, belongs to renewable resource and utilizes technical field.
Background technology
Mankind's maize planting has long history, and in China, corn seeding area is only second to paddy and wheat, in cereal crops
Occupy the 3rd.Annual also to produce substantial amounts of maize peel in addition to harvesting corn grain is as grain, this is a huge valuable money
Source, but for a long time, reasonable effective exploitation is not obtained and is utilized.Maize peel is mostly used in cooking and heating, remaining then discarded field
Between the edge of a field or burning, not only frequently cause the accidents such as fire, severe contamination environment also results in the great wasting of resources.To corn
Skin carries out degumming, obtains corn wrapper fibre, realizes the recycling of discarded maize peel, with environmental protection and sustainable development
Double meaning.The corn wrapper fibre of degumming can be used as packing material, braid and can be additionally used in textile industry.
At present, maize peel degumming mainly uses alkali degumming, although has using chemical-biological combination degumming, but plays a major role
Or alkali process, environmental pollution is more serious, larger to fibre damage.
The content of the invention
Present invention aims at provide a kind of green, environmental protection, the corn wrapper fibre Degumming method small to fibre damage.
Technical scheme：To achieve the above object, the present invention-a kind of microbiological degumming method of corn wrapper fibre, including with
Lower processing step：
（1）Select without the maize peel that goes mouldy, remove the debris such as dust on maize peel；
（2）Dipping pretreatment is carried out to corn wrapper fibre, bath raio is 1:10~15（w/v）, Nekal BX concentration is 1 ~ 2g/L, place
The reason time is 3 ~ 5h；
（3）Corn wrapper fibre microbial degumming liquid is prepared, by aspergillus niger（Aspergillus niger）HYA4 is sent out using solid
Ferment medium culture, fermentate are used as degumming liquid through extraction, centrifugation gained supernatant；
Described aspergillus niger（Aspergillus niger）HYA4, preserving number is CGMCCNo.1897；
Described solid fermentation condition of culture is：By aspergillus niger（Aspergillus niger）HYA4 is inoculated in solid fermentation training
Support in base, 72h is cultivated at 32 ~ 36 DEG C.
Wherein described solid fermentation culture medium is：Wheat bran: straw=3: 2, ammonium sulfate addition is 5.5%, material-water ratio 1:
3, initial pH5.5, cultivation temperature are 36 DEG C, and cultivation cycle is 72 h.
Described degumming liquid and preparation method thereof is：With water addition ratio 1:20, to aspergillus niger（Aspergillus niger）HYA4 consolidates
Add water extraction 1.5h in body fermentation culture medium, is then centrifuged for, centrifugal rotational speed is 3000r/min, centrifugation time is 10min.
Described aspergillus niger（Aspergillus niger）Also include culture presevation step before HYA4 solid fermentations, it is described
Culture presevation step be specially：Storage medium is：Sodium nitrate 3g/L, the g/L of dipotassium hydrogen phosphate 1, the g/L of magnesium sulfate 0.5, chlorine
Change the g/L of potassium 0.5, the g/L of ferrous sulfate 0.01, the g/L of sucrose 30, the g/L of agar 20；Cultivation temperature is 36 DEG C, after culture 5-7d
It is positioned over preservation in 4 DEG C of refrigerators.
（4）The microbial degumming of corn wrapper fibre, by step（2）Pretreated corn wrapper fibre is placed in step（3）Obtain
In the degumming liquid obtained, bath raio is 1:10~15（w/v）, degumming temperature is 30 ~ 36 DEG C, and processing time is：32~36h；
（5）Potassium hydroxide solution soaks, by step（4）It is 0.5M/L that corn wrapper fibre after processing, which adds concentration of potassium hydroxide,
Urea concentration for 0.5 M/L solution in, 0.5 ~ 1h of soak time；
（6）Washing and drying, by the maize peel washing after potassium hydroxide treatment, is dried.
Beneficial effects of the present invention are：The present invention is by aspergillus niger（Aspergillus niger）HYA4 is de- for maize peel
Glue, environmental pollution is lighter, and energy consumption is small；Solid fermentation culture medium raw material wide material sources, cheap, degumming cost is relatively low, hair
Ferment process is more extensive, it is easy to operate；Obtained corn wrapper fibre residual gum content is smaller, and fibre damage is small, with large-scale production
Prospect.
Embodiment
Embodiment 1
（1）Select without the maize peel that goes mouldy, remove the debris such as dust on maize peel；
（2）Dipping pretreatment is carried out to corn wrapper fibre, bath raio is 1:10（w/v）, Nekal BX concentration is 2g/L, processing time
For 4h；
（3）By aspergillus niger（Aspergillus niger）HYA4 is inoculated in solid fermentation culture medium, and culture medium composition is wheat bran:
Straw=3: 2, ammonium sulfate addition is 5.5%, material-water ratio 1: 3, initial pH5.5.Cultivated at 36 DEG C and terminate fermentation after 72h, with
Water addition ratio 1:20, to aspergillus niger（Aspergillus niger）Add water extraction 1.5h, Ran Houli in HYA4 solid fermentation cultures
The heart, centrifugal rotational speed is 3000r/min, and centrifugation time is 10min, and gained supernatant is used as degumming liquid.
（4）The microbial degumming of corn wrapper fibre, by step（2）Pretreated corn wrapper fibre is placed in step（3）Obtain
In the degumming liquid obtained, bath raio is 1:15（w/v）, degumming temperature is 32 DEG C, and processing time is 36h；
（5）Potassium hydroxide solution soaks, by step（4）Maize peel after processing, which drenches, removes unnecessary degumming liquid, is placed in potassium hydroxide
In solution, concentration of potassium hydroxide is 0.5M/L, and urea concentration is 0.5 M/L, soak time 0.5h；
（6）Washing and drying, by maize peel washing, the 80 DEG C of dryings after potassium hydroxide treatment.
Corn wrapper fibre residual gum content after degumming is 18.24%, and fracture strength is 1.63cN/dtex.
Embodiment 2
（1）Select without the maize peel that goes mouldy, remove the debris such as dust on maize peel；
（2）Dipping pretreatment is carried out to corn wrapper fibre, bath raio is 1:15（w/v）, Nekal BX concentration is 1.5g/L, during processing
Between be 5h；
（3）By aspergillus niger（Aspergillus niger）HYA4 is inoculated in solid fermentation culture medium, and culture medium composition is wheat bran:
Straw=3: 2, ammonium sulfate addition is 5.5%, material-water ratio 1: 3, initial pH5.5.Cultivated at 36 DEG C and terminate fermentation after 72h, with
Water addition ratio 1:20, to aspergillus niger（Aspergillus niger）Add water extraction 1.5h, Ran Houli in HYA4 solid fermentation cultures
The heart, centrifugal rotational speed is 3000r/min, and centrifugation time is 10min, and gained supernatant is used as degumming liquid.
（4）The microbial degumming of corn wrapper fibre, by step（2）Pretreated corn wrapper fibre is placed in step（3）Obtain
In the degumming liquid obtained, bath raio is 1:10（w/v）, degumming temperature is 32 DEG C, and processing time is 32h；
（5）Potassium hydroxide solution soaks, by step（4）Maize peel after processing, which drenches, removes unnecessary degumming liquid, is placed in potassium hydroxide
In solution, concentration of potassium hydroxide is 0.5M/L, and urea concentration is 0.5 M/L, soak time 1h；
（6）Washing and drying, by maize peel washing, the 80 DEG C of dryings after potassium hydroxide treatment.
Corn wrapper fibre residual gum content after degumming is 20.43%, and fracture strength is 1.79cN/dtex.
Embodiment 3
（1）Select without the maize peel that goes mouldy, remove the debris such as dust on maize peel；
（2）Dipping pretreatment is carried out to corn wrapper fibre, bath raio is 1:10（w/v）, Nekal BX concentration is 2g/L, processing time
For 5h；
（3）By aspergillus niger（Aspergillus niger）HYA4 is inoculated in solid fermentation culture medium, and culture medium composition is wheat bran:
Straw=3: 2, ammonium sulfate addition is 5.5%, material-water ratio 1: 3, initial pH5.5.Cultivated at 32 DEG C and terminate fermentation after 72h, with
Water addition ratio 1:20, to aspergillus niger（Aspergillus niger）Add water extraction 1.5h, Ran Houli in HYA4 solid fermentation cultures
The heart, centrifugal rotational speed is 3000r/min, and centrifugation time is 10min, and gained supernatant is used as degumming liquid.
（4）The microbial degumming of corn wrapper fibre, by step（2）Pretreated corn wrapper fibre is placed in step（3）Obtain
In the degumming liquid obtained, bath raio is 1:15（w/v）, degumming temperature is 36 DEG C, and processing time is 36h；
（5）Potassium hydroxide solution soaks, by step（4）Maize peel after processing, which drenches, removes unnecessary degumming liquid, is placed in potassium hydroxide
In solution, concentration of potassium hydroxide is 0.5M/L, and urea concentration is 0.5 M/L, soak time 1h；
（6）Washing and drying, by maize peel washing, the 80 DEG C of dryings after potassium hydroxide treatment.
Corn wrapper fibre residual gum content after degumming is 19.61%, and fracture strength is 1.68cN/dtex.
Claims (1)
1. a kind of microbiological degumming method of corn wrapper fibre, it is characterised in that described method comprises the following steps：
（1）Select without the maize peel that goes mouldy, remove the debris such as dust on maize peel；
（2）Dipping pretreatment is carried out to corn wrapper fibre, bath raio is 1:10~15（w/v）, Nekal BX concentration is 1 ~ 2g/L, place
The reason time is 3 ~ 5h；
（3）The preparation of corn wrapper fibre microbial degumming liquid, by aspergillus niger（Aspergillus niger）HYA4 is sent out using solid
Ferment medium culture, fermentate are used as degumming liquid through extraction, centrifugation gained supernatant；
Described aspergillus niger（Aspergillus niger）HYA4, preserving number is CGMCCNo.1897；
Described solid fermentation culture medium and condition of culture are：Wheat bran: straw=3: 2, ammonium sulfate addition is 5.5%, material-water ratio 1
: 3, initial pH5.5, cultivation temperature are 32 ~ 36 DEG C, and cultivation cycle is 72 h；
Described degumming liquid and preparation method thereof is：With water addition ratio 1:20, to aspergillus niger（Aspergillus niger）HYA4 solids are sent out
Add water extraction 1.5h in ferment culture, is then centrifuged for, centrifugal rotational speed is 3000r/min, centrifugation time is 10min；
Described aspergillus niger（Aspergillus niger）Also include culture presevation step, described bacterium before HYA4 solid fermentations
Planting preservation steps is specially：Storage medium is：Sodium nitrate 3g/L, the g/L of dipotassium hydrogen phosphate 1, the g/L of magnesium sulfate 0.5, potassium chloride
0.5 g/L, the g/L of ferrous sulfate 0.01, the g/L of sucrose 30, the g/L of agar 20；Cultivation temperature is 32 ~ 36 DEG C, is put after culture 5-7d
It is placed in preservation in 4 DEG C of refrigerators；
（4）The microbial degumming of corn wrapper fibre, by step（2）Pretreated corn wrapper fibre is placed in step（3）Obtain
In degumming liquid, bath raio is 1:10~15（w/v）, degumming temperature is 30 ~ 36 DEG C, and processing time is：32~36h；
（5）Potassium hydroxide solution soaks, by step（4）Maize peel after microbial degumming, which is placed in potassium hydroxide solution, to be soaked,
The concentration of potassium hydroxide is 0.5M/L, and urea concentration is 0.5 M/L, 0.5 ~ 1h of soak time；
（6）Washing and drying, by the maize peel washing after potassium hydroxide treatment, is dried.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611187045.XA CN107299398A (en) | 2016-12-21 | 2016-12-21 | A kind of microbiological degumming method of corn wrapper fibre |
| CN201711204859.4A CN107740194B (en) | 2016-12-21 | 2017-11-27 | Microbial degumming method for corn bran fiber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611187045.XA CN107299398A (en) | 2016-12-21 | 2016-12-21 | A kind of microbiological degumming method of corn wrapper fibre |
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| CN107299398A true CN107299398A (en) | 2017-10-27 |
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| CN201611187045.XA Pending CN107299398A (en) | 2016-12-21 | 2016-12-21 | A kind of microbiological degumming method of corn wrapper fibre |
| CN201711204859.4A Active CN107740194B (en) | 2016-12-21 | 2017-11-27 | Microbial degumming method for corn bran fiber |
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| CN201711204859.4A Active CN107740194B (en) | 2016-12-21 | 2017-11-27 | Microbial degumming method for corn bran fiber |
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| CN108977895B (en) * | 2018-09-29 | 2020-09-29 | 齐齐哈尔大学 | Method for preparing corn bract fiber by using biological enzyme |
| WO2023225135A1 (en) * | 2022-05-17 | 2023-11-23 | Trace Femcare, Inc. | Hemp fiber extraction and products using green degumming |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US8956853B2 (en) * | 2007-01-30 | 2015-02-17 | Bunge Oils, Inc. | Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases |
| CN100580081C (en) * | 2007-03-21 | 2010-01-13 | 齐齐哈尔大学 | Degelatinized enzyme made from Aspergillus niger and its use in degumming of fiberflax |
| CN101805725B (en) * | 2009-05-13 | 2012-05-16 | 齐齐哈尔大学 | Complex enzyme generated by aspergillus niger and application thereof in boiling-off of flax roving |
| CN104032379B (en) * | 2014-06-30 | 2015-12-30 | 青岛晟任现代农业发展有限公司 | A kind of preparation method of corn wrapper fibre and the corn wrapper fibre obtained by the method |
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| CN107740194A (en) | 2018-02-27 |
| CN107740194B (en) | 2020-03-17 |
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