CN107296775A

CN107296775A - Skin matrix, preparation method and applications and cosmetics and preparation method thereof

Info

Publication number: CN107296775A
Application number: CN201710747559.4A
Authority: CN
Inventors: 陈杰; 裴运林; 聂艳峰; 刘露
Original assignee: Guangzhou Pill Biotechnology Co Ltd
Current assignee: Guangzhou Pill Biotechnology Co Ltd
Priority date: 2017-08-25
Filing date: 2017-08-25
Publication date: 2017-10-27

Abstract

The present invention discloses a kind of skin matrix, preparation method and applications and cosmetics and preparation method thereof, is related to cosmetic technical field, with improve existing product can not complete barrier ultraviolet, visible ray and infrared ray to the technical problem of skin damage.Skin matrix of the present invention is main composed of the following components according to mass percentage：Propane diols 10 20%, pentanediol 3 5%, Flos Buddlejae extract 5 10%, tetrahydrochysene methylpyrimidine carboxylic acid 1 5%, gentian root extract 5 10%, surplus is water.Skin matrix of the present invention passes through reasonable compatibility between each component, the effect of Synergistic can be realized, effectively stop photo-aging and damage of the skin caused by ultraviolet, visible ray or infrared ray, strengthen the barrier action of skin, nutrition can also be provided by the effective Transdermal absorption of skin and for skin, while having effects that to resist photic damage comprehensively.

Description

Skin matrix, preparation method and applications and cosmetics and preparation method thereof

Technical field

The present invention relates to cosmetic technical field, in particular to a kind of skin matrix, preparation method and applications and Cosmetics and preparation method thereof.

Background technology

Skin is directly contacted as the maximum organ of human body with external environment, is one of the target organ of various light source effects. Since ancient times, how people protects skin not damaged by sunshine if being directed to studying always.First is used from ancient Egyptian Mineral suncream antiultraviolet exquisiter till now (UVA/UVB) organic sunscreen frost, either in terms of sun photoprotection, also In terms of being skin physiology research, we achieve significant progress.

About 5% is ultraviolet in sunshine, and about 40-45% is visible ray, and about 50% is the longer infrared part of wavelength. According to the difference of wavelength, ultraviolet includes UVA, UVB and UVC again, and wherein UVA wavelength is most long, reaches 320~400 nanometers, UVB wavelength is 290~320 nanometers, and UVC is then just absorbed before the earth is reached by ozone layer.Do not inhaled by ozone layer Earth surface can be irradiated to by receiving the UVA fallen and UVB, brought injury to skin and eyes, allowed premature skin ageing, or even cause skin Skin cancer.Mainly, UVA is by oxidation reaction, influence DNA, protein and lipid, and suppresses immune response；UVB mainly results in solarization Spot and DNA dimers are formed, and are easily caused gene mutation.In addition, in recent years it is found that except ultraviolet, it is seen that light and infrared Line is also relevant with skin oxidative damage, and infrared ray is relevant with the fuel factor of skin.Visible ray easily causes DNA single-strand breaks And reactive oxygen species (ROS) are produced, so as to trigger DNA damage, cause the photo-aging of skin.Infrared ray can increase matrix gold The expression of Proteases -1 (MMP-1) in the dermis, and can substantially reduce the antioxidant content of human body skin.

However, cosmetics research at present is principally dedicated to the exploitation of sun-screening agent, effectively to stop the exposure to UVA and UVB, Damage for visible ray and infrared ray to skin is not protected effectively.Therefore skin matrix, preparation method and applications are studied With cosmetics and preparation method thereof, each wave band light is resisted comprehensively to realize, it is to avoid skin tape is damaged with important meaning Justice.

In view of this, it is special to propose the present invention.

The content of the invention

The present invention first purpose be to provide a kind of skin matrix, with improve existing product can not complete barrier it is ultraviolet The technical problem of line, visible ray and infrared ray to skin damage.The matrix realizes Synergistic by reasonable compatibility between each component Effect, nutrition can be provided by skin Transdermal absorption for skin, while having effects that to resist photic damage comprehensively.

Second object of the present invention is to provide a kind of preparation method of above-mentioned skin matrix, and this method makes full use of this The component of invention, results in the skin matrix product of uniform properties, steady quality, simple with method, it is easy to operation it is excellent Gesture, suitable for standardized production and popularization and application.

Third object of the present invention is to provide a kind of application of skin matrix of the present invention in cosmetics are prepared.

Fourth object of the present invention, which is that offer is a kind of, includes the cosmetics of the skin matrix, for example, emulsion, cream Frost, toner, Essence, muffin or sunscreen productses, the cosmetics can effectively be absorbed by skin, be skin supply nutrition, simultaneously Have effects that to resist photic damage comprehensively.

The present invention the 5th purpose be to provide a kind of preparation method of the cosmetics, this method makes full use of this hair The component of bright cosmetics, results in the cosmetics of uniform properties, steady quality, simple with method, it is easy to the advantage of operation, Suitable for standardized production and popularization and application.

In order to realize the above-mentioned purpose of the present invention, spy uses following technical scheme：

The present invention provides a kind of skin matrix, according to mass percentage, main composed of the following components：Propane diols 10- 20%, pentanediol 3-5%, Flos Buddlejae extract 5-10%, tetrahydrochysene methylpyrimidine carboxylic acid 1-5%, gentian root extract 5- 10%, surplus is water.

Alternatively, skin matrix of the present invention, it is main composed of the following components according to mass percentage：The third two Alcohol 12-20%, pentanediol 4-5%, Flos Buddlejae extract 6-10%, tetrahydrochysene methylpyrimidine carboxylic acid 2-5%, gentian root extract 6-10%, surplus is water.

Preferably, skin matrix of the present invention, it is main composed of the following components according to mass percentage：The third two Alcohol 15-20%, pentanediol 4-5%, Flos Buddlejae extract 6-9%, tetrahydrochysene methylpyrimidine carboxylic acid 2-4%, gentian root extract 7-9%, surplus is water.

The present invention also provides a kind of preparation method of the skin matrix simultaneously, comprises the following steps：By the water of formula ratio, Propane diols and pentanediol Hybrid Heating, add Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid and gentian root and extract after cooling Thing is uniformly mixed so as to obtain skin matrix.

Alternatively, in the preparation method of skin matrix of the present invention, temperature after heating is 85-90 DEG C；And/or, drop Temperature is 35-40 DEG C after temperature.

Alternatively, the preparation method of the skin matrix, comprises the following steps：

(a) by the water of formula ratio, propane diols and pentanediol Hybrid Heating and stir, be then incubated, obtain clear solution；

(b) Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid and gentian root extract are added after cooling, is divided after stirring From obtaining skin matrix.

Alternatively, in the preparation method of skin matrix of the present invention, step (a), temperature after heating is 85-90 DEG C； And/or, mixing speed is 300-400r/min；And/or, soaking time is 20-30min；And/or, in step (b), after cooling Temperature is 35-40 DEG C；And/or, mixing speed is 800-1000r/min, and mixing time is 10-15min；And/or, separation is used The mode of filtering, filtering mesh number is 150-250 mesh.

Preferably, the preparation method of the skin matrix, comprises the following steps：

(a) water, propane diols and pentanediol of formula ratio are mixed and heated to 85-90 DEG C, mixing speed is 300-400r/ Min, is incubated 20-30min, obtains clear solution；

(b) addition Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid and gentian root extract after 35-40 DEG C is cooled to stir Mix, mixing speed is 800-1000r/min, mixing time is 10-15min, then with 150-200 mesh filter-cloth filterings, obtain skin care Matrix.

In addition, the present invention also provides application of the skin matrix in cosmetics are prepared.

In addition, the present invention also provides a kind of cosmetics, including skin matrix of the present invention；Preferably, it is described to make up Product include emulsion, cream, toner, Essence, muffin or sunscreen productses；Preferably, matter of the skin matrix in cosmetics Amount percentage is 20-30%.

Alternatively, cosmetics of the present invention, it is main composed of the following components according to mass percentage：

Component A：Methyl sesquistearate 1-2%, PEG-20 methyl sesquistearate 0.5-1% is sweet Oleostearin acid esters/PEG-100 stearate 1-2%, caprylic/capric triglyceride 2-5%, dimethyl silicone polymer 2-5%, mountain Yu alcohol 0.5-2%, carbonic acid dioctyl ester 3-5%, Nipasol 0.05-0.2%；

B component：Glycerine 5-10%, butanediol 2-5%, erythrite 1-3%, Sodium Hyaluronate 0.01-0.03%, allantoin 0.1-0.4%, carbomer 0.1-0.5%, EDETATE SODIUM 0.01-0.05%, surplus is water；

Component C：Triethanolamine 0.1-0.5%；

D components：Essence optional the skin matrix 20-30% and 0.01-0.03%.

Preferably, according to mass percentage, the cosmetics are mainly composed of the following components：

Component A：Methyl sesquistearate 1.5-2%, PEG-20 methyl sesquistearate 0.5-1%, Stearine/PEG-100 stearate 1-2%, caprylic/capric triglyceride 3-4%, dimethyl silicone polymer 3-5%, Docosyl alcohol 1-2%, carbonic acid dioctyl ester 3-5%, Nipasol 0.01-0.2%；

B component：Glycerine 6-9%, butanediol 3-5%, erythrite 1-3%, Sodium Hyaluronate 0.01-0.03%, allantoin 0.2-0.4%, carbomer 0.2-0.5%, EDETATE SODIUM 0.02-0.05%, surplus is water；

Component C：Triethanolamine 0.2-0.5%；

D components：Essence optional the skin matrix 25-30% and 0.02-0.03%.

Finally, the present invention also provides a kind of preparation method of the cosmetics, comprises the following steps：It will be mixed by component A Obtained oil-phase component carries out homogeneous with the water-phase component being uniformly mixed so as to obtain by B component, then adds component C homogeneous again, adds D Cosmetics are obtained after component and optional essence, homogeneous.

Preferably, the preparation method of the cosmetics comprises the following steps：

(a) each material in component A is added into oil phase pot, stirring is warming up to 85-90 DEG C, is then incubated standby, obtains oil Phase constituent；

(b) each material in B component is added into aqueous phase pot, stirring is warming up to 85-90 DEG C, is then incubated standby, obtains water Phase constituent；

(c) oil-phase component suction emulsification pot is kept vacuum and stirred again after water-phase component suction emulsification pot is stirred Mix speed constant, homogeneous 3-5min；

(d) component C is added after cooling, then carries out homogeneous 3-5min；

(e) continue to cool, stir 3-5min, homogeneous 1-3min after adding D components and optional essence, obtain cosmetics.

Preferably, in step (a), mixing speed is 800-1000r/min；And/or, soaking time is 10-15min.

Preferably, in step (b), mixing speed is 800-1000r/min；And/or, soaking time is 15-20min.

Preferably, in step (c), mixing speed is 400-600r/min；And/or, vacuum be -0.04~- 0.06MPa；And/or, homogeneous speed is 2000-3000r/min.

Preferably, in step (d), temperature is 52-58 DEG C after cooling；And/or, homogeneous speed is 2000-3000r/min.

Preferably, in step (e), the temperature after cooling is 42-48 DEG C；And/or, mixing speed is 400-600r/min； And/or, homogeneous speed is 2000-3000r/min.

Compared with prior art, beneficial effects of the present invention are：

(1) Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid and gentian root extract are selected in skin matrix of the present invention Coordinate Deng functional components, and with propane diols, pentanediol and water, by reasonable compatibility between each component, Synergistic can be realized Effect, effectively stop photo-aging and damage of the skin caused by ultraviolet, visible ray or infrared ray, strengthen the barrier of skin Effect, protects the skin from light injury.Skin matrix of the present invention can provide nutrition by the effective Transdermal absorption of skin and for skin, Have effects that to resist photic damage comprehensively simultaneously.

(2) preparation method of skin matrix of the present invention is simple, easy to operate, by heating, stirring and separation, can make each Fully mixed between the active ingredient of component, be conducive to active component fully to discharge, ensure that it is final obtain property it is homogeneous and The skin matrix product of steady quality, the preparation method is scientific and normal, it is easy to operate, suitable for standardized production and popularization and application.

(3) skin matrix of the present invention can be added to emulsion, cream, toner, Essence, muffin or sun-proof as raw material In the cosmetics such as product, have effects that to resist photic damage comprehensively.

(4) cosmetics can not only be made saturating by skin by adding the skin matrix of certain content in cosmetics of the present invention Skin absorbs, and provides nutrition for skin, also makes cosmetics have effects that to resist photic damage comprehensively.

(5) preparation method of cosmetics of the present invention is simple, easy to operate, ensure that and final obtains that property is homogeneous and quality Stable cosmetics, the preparation method is scientific and normal, it is easy to operate, suitable for standardized production and popularization and application.

Embodiment

Embodiment of the present invention is described in detail below in conjunction with embodiment and embodiment, but this area skill Art personnel will be understood that following embodiments and embodiment are merely to illustrate the present invention, and are not construed as the model of the limitation present invention Enclose.Unreceipted actual conditions person, the condition advised according to normal condition or manufacturer is carried out.Agents useful for same or the unreceipted life of instrument Manufacturer person is produced, is the conventional products that can be obtained by commercially available purchase.

According to the first aspect of the invention, embodiment of the present invention provides a kind of skin matrix, according to mass percent Meter, it is main composed of the following components：Propane diols 10-20%, pentanediol 3-5%, Flos Buddlejae extract 5-10%, tetrahydrochysene methyl is phonetic Pyridine carboxylic acid 1-5%, gentian root extract 5-10%, surplus is water.

In skin matrix of the present invention, " mainly by ... " means it in addition to the component, can also include other components, this A little other components assign the skin matrix different characteristics.In addition, " mainly by ... " of the present invention, can be with Replace with enclosed " being " or " by ... ".

Propane diols and pentanediol are the commercially available prod of purchase.

Butterflybush flower (BUDDLEJA OFFICINALIS) extract, for the commercially available prod of purchase, purchase is public from French green section Department.In the present invention, Flos Buddlejae extract has antimicrobial antiphlogistic, and sun-proof moisturizing maintains cutaneous immunisation balance and redox to put down Effect of weighing apparatus.Generally, Flos Buddlejae extract is extracted or is heated to reflux or supercritical fluid in room temperature after butterflybush flower is crushed The extract that extraction or other manner are obtained, the liquid that Extraction solvent is mixed for water with ethanol with arbitrary proportion, or ether, stone One or more miscible liquid in any proportion in the organic solvents such as oily ether, chloroform or ethyl acetate, or liquid Carbon dioxide.

Tetrahydrochysene methylpyrimidine carboxylic acid, buys from Bitop AG companies of Germany.In the present invention, tetrahydrochysene methylpyrimidine carboxylic acid is A kind of cell resistance to compression protective agent and multifunction activity composition, play a part of chemical mediator, to cell in inverse ring border in the cell In have the protective effect of stability, can also stablize the structure of zymoprotein in organism, with the anti-ageing function of skin-active；It can also provide Good moisturizing and sun-screening function, repair skin barrier, prevent photo-aging, improve skin wrinkle, and have the work of skin whitening With；Moreover it is possible to neutrophil leucocyte be protected, with antiinflammatory action.

Gentian (GENTIANA LUTEA) root extract, buys from Te Kenuobao Co., Ltds of Japan.In the present invention With moisturizing, suppress melanin generation and the effect of anti-inflammatory.

Preferably, water is deionized water.

Alternatively, it is 10% that the mass percent of propane diols is typical but non-limiting, 11%, 12%, 13%, 14%, 15%th, 16%, 17%, 18%, 19% or 20%.

Alternatively, it is 3%, 4% or 5% that the mass percent of pentanediol is typical but non-limiting.

Alternatively, the mass percent of Flos Buddlejae extract it is typical but non-limiting be 5%, 6%, 7%, 8%, 9% or 10%.

Alternatively, the mass percent of tetrahydrochysene methylpyrimidine carboxylic acid it is typical but non-limiting be 1%, 2%, 3%, 4% or 5%.

Alternatively, it is 5%, 6%, 7%, 8%, 9% that the mass percent of gentian root extract is typical but non-limiting Or 10%.

It should be noted that in the component of skin matrix of the present invention, surplus is that water refers to removing propane diols, penta 2 Surplus outside alcohol, Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid, gentian root extract and optional other components is Water, i.e., in the component of skin matrix of the present invention, water and propane diols, pentanediol, Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic The mass percent sum of acid, gentian root extract and optional other components is 100%.

Compared with prior art, the beneficial effect for the combination for protecting the skin from light injury that embodiment of the present invention is provided For：

Selection Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid and Huanglong in the skin matrix that embodiment of the present invention is provided The functional components such as courage root extract, and coordinate with propane diols, pentanediol and water, pass through reasonable compatibility between each component, Neng Goushi The effect of existing Synergistic, effectively stops photo-aging and damage of the skin caused by ultraviolet, visible ray or infrared ray, enhancing The barrier action of skin, protects the skin from light injury.The skin matrix that embodiment of the present invention is provided can be effective by skin Transdermal absorption and nutrition is provided for skin, while having effects that to resist photic damage comprehensively.

Alternatively, the skin matrix that embodiment of the present invention is provided, according to mass percentage, mainly by following components group Into：Propane diols 12-20%, pentanediol 4-5%, Flos Buddlejae extract 6-10%, tetrahydrochysene methylpyrimidine carboxylic acid 2-5%, gentian Root extract 6-10%, surplus is water.

Preferably, the skin matrix that embodiment of the present invention is provided, according to mass percentage, mainly by following components group Into：Propane diols 15-20%, pentanediol 4-5%, Flos Buddlejae extract 6-9%, tetrahydrochysene methylpyrimidine carboxylic acid 2-4%, gentian root Extract 7-9%, surplus is water.

By the adjustment and optimization to each group distribution ratio, the protection effect of skin matrix can be further enhanced, effectively resistance Ultraviolet, visible ray or infrared ray are kept off, prevents from causing photo-aging and damage, strengthens the barrier action of skin.

According to the second aspect of the invention, embodiment of the present invention provides a kind of preparation method of above-mentioned skin matrix, Comprise the following steps：By the water of formula ratio, propane diols and pentanediol Hybrid Heating, Flos Buddlejae extract, tetrahydrochysene are added after cooling Methylpyrimidine carboxylic acid and gentian root extract are uniformly mixed so as to obtain skin matrix.

Alternatively, in the preparation method for the skin matrix that embodiment of the present invention is provided, temperature after heating is 85-90 DEG C； And/or, temperature is 35-40 DEG C after cooling.

Alternatively, in the preparation method for the skin matrix that embodiment of the present invention is provided, temperature after heating is typical but non- Restricted is 85 DEG C, 86 DEG C, 87 DEG C, 88 DEG C, 89 DEG C or 90 DEG C.

Alternatively, in the preparation method for the skin matrix that embodiment of the present invention is provided, the temperature after cooling is typical but non- Restricted is 35 DEG C, 36 DEG C, 37 DEG C, 38 DEG C, 39 DEG C or 40 DEG C.

Alternatively, the preparation method for the skin matrix that embodiment of the present invention is provided, comprises the following steps：

The preparation method for the skin matrix that embodiment of the present invention is provided is simple, easy to operate, by heating, stirring and divide From fully being mixed between the active ingredient that can make each component, be conducive to active component fully to discharge, ensure that final acquisition Property is homogeneous and skin matrix product of steady quality, and the preparation method is scientific and normal, it is easy to operate, suitable for standardized production and Popularization and application.

Alternatively, in the preparation method for the skin matrix that embodiment of the present invention is provided, step (a), heating-up temperature is 85- 90℃；And/or, in step (a), mixing speed is 300-400r/min；And/or, in step (a), soaking time is 20- 30min。

Alternatively, in the preparation method for the skin matrix that embodiment of the present invention is provided, step (a), heating-up temperature typical case But non-limiting is 85 DEG C, 86 DEG C, 87 DEG C, 88 DEG C, 89 DEG C or 90 DEG C；And/or, in step (a), mixing speed is typical but non-limit Property processed is 300r/min, 350r/min or 400r/min；And/or, in step (a), soaking time is typical but non-limiting to be 20min, 21min, 22min, 23min, 24min, 25min, 26min, 27min, 28min, 29min or 30min.

Alternatively, in the preparation method for the skin matrix that embodiment of the present invention is provided, step (b), temperature is after cooling 35-40℃；And/or, in step (b), mixing speed is 800-1000r/min, and mixing time is 10-15min；And/or, step (b) in, separation is by the way of filtering, and filtering mesh number is 150-250 mesh.

Alternatively, in the preparation method for the skin matrix that embodiment of the present invention is provided, step (b), the temperature after cooling It is typical but it is non-limiting be 35 DEG C, 36 DEG C, 37 DEG C, 38 DEG C, 39 DEG C or 40 DEG C；And/or, in step (b), mixing speed typical case but Non-limiting is 800r/min, 850r/min, 900r/min, 950r/min or 1000r/min, and mixing time is typical but unrestricted Property be 10min, 11min, 12min, 13min, 14min or 15min；And/or, in step (b), filtering mesh number is typical but unrestricted Property be 150 mesh, 200 mesh or 250 mesh.

By the existing mixed liquor for obtaining water, propane diols and pentanediol at relatively high temperatures under given conditions, three can be made Kind of composition is fully merged uniformly, then cool to added after lower temperature Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid and Gentian root extract, can either make Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid and gentian root extract fully therewith Preceding mixed liquor fusion, can be sufficiently reserved the active component in each component again, it is to avoid preparation process high temperature to effectively into Divide and damage, it is final to obtain property stable homogeneous and protect skin from the composition of light injury comprehensively；In addition, last filtering Step can effectively improve the present invention and protect skin from the purity of the composition product of light injury, it is ensured that higher quality.

Preferably, the preparation method for the skin matrix that embodiment of the present invention is provided, comprises the following steps：

(a) water, propane diols and pentanediol of formula ratio are mixed and heated to 85 DEG C, mixing speed is 300r/min, are incubated 30min, obtains clear solution；

(b) it is cooled to after 38 DEG C and adds Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid and the stirring of gentian root extract, Mixing speed is 1000r/min, and mixing time is 10min, then with 200 mesh filter-cloth filterings, obtains skin matrix.

According to the third aspect of the present invention, embodiment of the present invention provides a kind of above-mentioned skin matrix and is preparing cosmetics In application.

The skin matrix that embodiment of the present invention is provided can be added to emulsion, cream, toner, elite as raw material In the cosmetics such as liquid, muffin or sunscreen productses, have effects that to resist photic damage comprehensively.

According to the fourth aspect of the present invention, embodiment of the present invention provides a kind of cosmetics, including above-mentioned skin matrix； Preferably, cosmetics include emulsion, cream, toner, Essence, muffin or sunscreen productses；Preferably, skin matrix is in cosmetics In mass percent be 20-30%.

By adding the skin matrix of certain content in the cosmetics that embodiment of the present invention is provided, cosmetic can not only be made Product provide nutrition by skin Transdermal absorption for skin, also make cosmetics have effects that to resist photic damage comprehensively.

Alternatively, it is 20% that mass percent of the above-mentioned skin matrix in cosmetics is typical but non-limiting, 21%, 22%th, 23%, 24%, 25%, 26%, 27%, 28%, 29% or 30%.

Alternatively, the cosmetics that embodiment of the present invention is provided, according to mass percentage, mainly by following components group Into：

Component C：Triethanolamine 0.1-0.5%；

D components：Essence optional skin matrix 20-30% and 0.01-0.03% of the present invention.

Preferably, according to mass percentage, the cosmetics that embodiment of the present invention is provided are mainly composed of the following components：

Component C：Triethanolamine 0.2-0.5%；

D components：Essence optional skin matrix 25-30% and 0.02-0.03% of the present invention.

By adding the skin matrix of the present invention of special ratios in cosmetics, it can make cosmetics that there is resistance light comprehensively Effect of damage is caused, and cosmetics can also effectively be absorbed by skin, nutrition be provided for skin, while mutually melting with other components Close, additionally it is possible to realize good replenishing water and preserving moisture, the effect of moisturizing skin lightening.

In the cosmetics that embodiment of the present invention is provided, " mainly by ... " means that it in addition to the component, can be wrapped Other components are included, these components assign the cosmetics different characteristics.In addition, it is of the present invention " main By ... ", it may be replaced by enclosed " being " or " by ... ".

Above-mentioned component A is oil phase and emulsifying agent, is aqueous phase after B component mixing, component C is pH adjusting agent, can effectively be adjusted The acid-base property of cosmetics is saved, simultaneously as D components are low temperature component, therefore addition D groups after last cooling are needed in the preparation Point, the active ingredient and remaining composition that ensure that D components are sufficiently mixed uniformly, are reduced the loss of active ingredient, are finally given Property stable homogeneous and the preferable cosmetics of quality.

Alternatively, it is 1% that the mass percent of methyl sesquistearate is typical but non-limiting, 1.2%, 1.5%th, 1.6%, 1.8% or 2%.

Alternatively, it is 0.5% that the percentage of PEG-20 methyls sesquistearate is typical but non-limiting, 0.6%, 0.7%th, 0.8%, 0.9% or 1%.

It is 1% that alternatively, the mass percent of stearine/PEG-100 stearates is typical but non-limiting, 1.2%th, 1.5%, 1.6%, 1.8% or 2%.

Alternatively, it is 2%, 3%, 4% or 5% that the mass percent of caprylic/capric triglyceride is typical but non-limiting.

Alternatively, it is 2% that the mass percent of dimethyl silicone polymer is typical but non-limiting, 2.5%, 3%, 3.5%, 4%th, 4.5% or 5%.

Alternatively, it is 0.5% that the mass percent of docosyl alcohol is typical but non-limiting, 0.6%, 0.8%, 1%, 1.2%, 1.5%th, 1.6%, 1.8% or 2%.

Alternatively, the mass percent of carbonic acid dioctyl ester it is typical but non-limiting be 3%, 3.5%, 4%, 4.5% or 5%.

Alternatively, it is 0.05% that the mass percent of Nipasol is typical but non-limiting, 0.08%, 0.1%, 0.12%th, 0.15%, 0.18% or 0.2%.

Alternatively, it is 5%, 6%, 7%, 8%, 9% or 10% that the mass percent of glycerine is typical but non-limiting.

Alternatively, it is 2%, 3%, 4% or 5% that the mass percent of butanediol is typical but non-limiting.

Alternatively, it is 1%, 1.5%, 2%, 2.5% or 3% that the mass percent of erythrite is typical but non-limiting.

Alternatively, it is 0.01%, 0.02% or 0.03% that the mass percent of Sodium Hyaluronate is typical but non-limiting.

Alternatively, it is 0.1%, 0.2%, 0.3% or 0.4% that the mass percent of allantoin is typical but non-limiting.

Alternatively, the mass percent of carbomer it is typical but non-limiting be 0.1%, 0.2%, 0.3%, 0.4% or 0.5%.

Alternatively, it is 0.01% that the mass percent of EDETATE SODIUM is typical but non-limiting, 0.02%, 0.03%, 0.04% or 0.05%.

Alternatively, the mass percent of triethanolamine it is typical but non-limiting be 0.1%, 0.2%, 0.3%, 0.4% or 0.5%.

Alternatively, it is 20% that the mass percent of skin matrix is typical but non-limiting, 21%, 22%, 23%, 24%, 25%th, 26%, 27%, 28%, 29% or 30%.

Alternatively, it is 0.01%, 0.02% or 0.03% that the mass percent of essence is typical but non-limiting.

Preferably, water is deionized water.

It should be noted that in the component of cosmetics of the present invention, surplus is that water refers to removing component A, component C, D components With the glycerine in B component, butanediol, erythrite, Sodium Hyaluronate, allantoin, carbomer, EDETATE SODIUM and optional other groups Surplus beyond point is water, i.e. water and component A, component C, D components and glycerine in B component, butanediol, erythrite, hyalomitome Sour sodium, allantoin, carbomer, the mass percent sum of EDETATE SODIUM and optional other components are 100%.

According to the fifth aspect of the present invention, embodiment of the present invention provides a kind of preparation method of above-mentioned cosmetics, bag Include following steps：Homogeneous is carried out by the oil-phase component being uniformly mixed so as to obtain by component A and by the water-phase component that B component is uniformly mixed so as to obtain, so Component C homogeneous again is added afterwards, and cosmetics are obtained after adding D components and optional essence, homogeneous.

Preferably, the preparation method for the cosmetics that embodiment of the present invention is provided comprises the following steps：

(d) component C is added after cooling, then carries out homogeneous 3-5min；

Homogeneous is also referred to as homogenate, be make dispersion micronized in suspension (or emulsion) system, homogenization it is treated Journey, this processing plays reduction dispersion yardstick and improves dispersion distributing homogeneity simultaneously.

By sequentially adding each component and by operations such as heating, stirring and homogeneous, can make to fill between each active ingredient Divide mixing, be conducive to active component fully to discharge.This method makes full use of the component of cosmetics of the present invention, results in property equal Even, steady quality cosmetics, it is simple with method, it is easy to the advantage of operation, suitable for standardized production and popularization and application.

Alternatively, in the preparation method for the cosmetics that embodiment of the present invention is provided, step (a), mixing speed is 800- 1000r/min；And/or, in step (a), soaking time is 10-15min.

Alternatively, the preparation method for the cosmetics that embodiment of the present invention is provided, in step (a), mixing speed typical case but Non-limiting is 800r/min, 850r/min, 900r/min, 950r/min or 1000r/min；And/or, in step (a), heating It is 85 DEG C, 86 DEG C, 87 DEG C, 88 DEG C, 89 DEG C or 90 DEG C that temperature is typical but non-limiting afterwards；And/or, in step (a), holding temperature It is typical but non-limiting for 10min, 11min, 12min, 13min, 14min or 15min.

Alternatively, in the preparation method for the cosmetics that embodiment of the present invention is provided, step (b), mixing speed is 800- 1000r/min；And/or, in step (b), soaking time is 15-20min.

Alternatively, in the preparation method for the cosmetics that embodiment of the present invention is provided, step (b), speed is mixed typical but non- Restricted is 800r/min, 850r/min, 900r/min, 950r/min or 1000r/min；And/or, in step (b), after heating Typical but non-limiting temperature is 85 DEG C, 86 DEG C, 87 DEG C, 88 DEG C, 89 DEG C or 90 DEG C；And/or, in step (b), holding temperature allusion quotation Type but it is non-limiting be 15min, 16min, 17min, 18min, 19min or 20min.

Alternatively, in the preparation method for the cosmetics that embodiment of the present invention is provided, step (c), mixing speed is 400- 600r/min；And/or, in step (c), vacuum is -0.04~-0.06MPa；And/or, in step (c), homogeneous speed is 2000-3000r/min。

Alternatively, the preparation method for the cosmetics that embodiment of the present invention is provided, in step (c), mixing speed typical case but Non-limiting is 400r/min, 450r/min, 500r/min, 550r/min or 600r/min；And/or, in step (c), vacuum Typical but non-limiting degree is -0.04MPa, -0.05MPa or -0.06MPa；And/or, in step (c), homogeneous speed typical case but Non-limiting is 2000r/min, 2500r/min or 3000r/min, homogenizing time it is typical but it is non-limiting for 3min, 4min or 5min。

Alternatively, in the preparation method for the cosmetics that embodiment of the present invention is provided, step (d), temperature is 52- after cooling 58℃；And/or, in step (d), homogeneous speed is 2000-3000r/min.

Alternatively, the preparation method for the cosmetics that embodiment of the present invention is provided is in step (d), temperature typical case after cooling But non-limiting is 52 DEG C, 53 DEG C, 54 DEG C, 55 DEG C, 56 DEG C, 57 DEG C or 58 DEG C；And/or, in step (d), homogeneous speed typical case But non-limiting is 2000r/min, 2500r/min or 3000r/min, and typical but non-limiting homogenizing time is 3min, 4min Or 5min.

Alternatively, in the preparation method for the cosmetics that embodiment of the present invention is provided, step (e), the temperature after cooling is 42-48℃；And/or, in step (e), mixing speed is 400-600r/min；And/or, in step (e), homogeneous speed is 2000-3000r/min。

Alternatively, the preparation method for the cosmetics that embodiment of the present invention is provided is in step (e), temperature typical case after cooling But non-limiting is 42 DEG C, 43 DEG C, 44 DEG C, 45 DEG C, 46 DEG C, 47 DEG C or 48 DEG C；And/or, in step (e), mixing speed typical case But non-limiting is 400r/min, 450r/min, 500r/min, 550r/min or 600r/min, mixing time is typical but non-limit Property processed is 3min, 4min or 5min；And/or, in step (e), homogeneous speed it is typical but it is non-limiting for 2000r/min, 2500r/min or 3000r/min, typical but non-limiting homogenizing time is 1min, 2min or 3min.

In the preparation method of cosmetics of the present invention, temperature, mixing speed, mixing time, are added by the way that selection is specific Matter speed and homogenizing time, can effectively ensure that each component is sufficiently mixed uniformly, it is to avoid the using effect of influence cosmetics, finally Prepare property stable homogeneous and with the cosmetics for protecting the skin from light injury effect comprehensively.

A preferred embodiment of the invention, the preparation method of cosmetics of the present invention comprises the following steps：

(a) component A is sequentially added into oil phase pot, opens stirring, mixing speed is 1000r/min, and be warming up to 85 DEG C, guarantor Warm 10min, obtains oil-phase component standby；

(b) B component is added into aqueous phase pot, opens stirring, mixing speed is 1000r/min, and be warming up to 85 DEG C, insulation 20min, obtains water-phase component standby；

(c) by after water-phase component suction emulsification pot, open stirring, mixing speed is 600r/min, then it is slow by oil phase into Divide suction emulsification pot, then keep vacuum -0.05MPa and mixing speed constant, open outer circulation homogenizer progress homogeneous, Homogenizing time is 5min, and homogeneous speed is 3000r/min；

(d) cooling water temperature is opened to 55 DEG C, component C is added, and is opened outer circulation homogenizer and is carried out homogeneous, homogenizing time For 3min, homogeneous speed is 2000r/min；

(e) continue to be cooled to 45 DEG C, add D components, stir 5min, mixing speed is 600r/min, is then turned on interior circulation Equipment homogeneous 1min, homogeneous speed is 2000r/min, and preparing includes the cosmetics of skin matrix of the present invention.

By selecting above-mentioned specific condition to be prepared, the cosmetics of property stable homogeneous are resulted in, and were prepared Journey only destroys the structure and activity of active ingredient, it is ensured that the using effect of cosmetics, effectively realizes to skin replenishing water and preserving moisture, profit Skin skin lightening and the effect for protecting the skin from light injury comprehensively.

In order to further appreciate that the present invention, with reference to specific embodiment and comparative example to method for product of the present invention and effect Elaborate.Each component of the present invention can be obtained by buying.

Embodiment 1

A kind of skin matrix, it is main composed of the following components according to mass percentage：Propane diols 20%, pentanediol 5%, Flos Buddlejae extract 7%, tetrahydrochysene methylpyrimidine carboxylic acid 3%, gentian root extract 8%, water 57%.

The preparation method of skin matrix, comprises the following steps：

(a) water, propane diols and pentanediol of formula ratio are mixed and heated to 88 DEG C and stirred, mixing speed is 400r/ Min, is then incubated 20min, obtains clear solution；

(b) it is cooled to after 40 DEG C and adds Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid and the stirring of gentian root extract, Mixing speed is 800r/min, and mixing time is 15min, then with 150 mesh filter-cloth filterings, obtains skin matrix.

Embodiment 2

A kind of skin matrix, it is main composed of the following components according to mass percentage：Propane diols 20%, pentanediol 5%, Flos Buddlejae extract 10%, tetrahydrochysene methylpyrimidine carboxylic acid 3%, gentian root extract 8%, water 54%.

The preparation method of skin matrix, comprises the following steps：

(1) water, propane diols and pentanediol of formula ratio are mixed and heated to 87 DEG C and stirred, mixing speed is 300r/ Min, is then incubated 25min, obtains clear solution；

(2) it is cooled to after 35 DEG C and adds Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid and the stirring of gentian root extract, Mixing speed is 900r/min, and mixing time is 12min, then with 250 mesh filter-cloth filterings, obtains skin matrix.

Embodiment 3

A kind of skin matrix, it is main composed of the following components according to mass percentage：Propane diols 20%, pentanediol 5%, Flos Buddlejae extract 7%, tetrahydrochysene methylpyrimidine carboxylic acid 5%, gentian root extract 8%, water 55%.

The preparation method of skin matrix, comprises the following steps：

(1) water, propane diols and pentanediol of formula ratio are mixed and heated to 85 DEG C and stirred, mixing speed is 300r/ Min, is then incubated 30min, obtains clear solution；

(2) it is cooled to after 38 DEG C and adds Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid and the stirring of gentian root extract, Mixing speed is 1000r/min, and mixing time is 10min, then with 200 mesh filter-cloth filterings, obtains skin matrix.

Embodiment 4

A kind of skin matrix, it is main composed of the following components according to mass percentage：Propane diols 20%, pentanediol 5%, Flos Buddlejae extract 7%, tetrahydrochysene methylpyrimidine carboxylic acid 3%, gentian root extract 10%, water 55%.

The preparation method of skin matrix, be the same as Example 3.

Embodiment 5

A kind of skin matrix, it is main composed of the following components according to mass percentage：Propane diols 10%, pentanediol 4%, Flos Buddlejae extract 5%, tetrahydrochysene methylpyrimidine carboxylic acid 4%, gentian root extract 7%, water 70%.

The preparation method of skin matrix, be the same as Example 3.

Embodiment 6

A kind of skin matrix, it is main composed of the following components according to mass percentage：Propane diols 15%, pentanediol 3%, Flos Buddlejae extract 8%, tetrahydrochysene methylpyrimidine carboxylic acid 1%, gentian root extract 5%, water 58%.

The preparation method of skin matrix, be the same as Example 3.

Comparative example 1

A kind of skin matrix, it is main composed of the following components according to mass percentage：Propane diols 20%, pentanediol 5%, Flos Buddlejae extract 0%, tetrahydrochysene methylpyrimidine carboxylic acid 3%, gentian root extract 10%, water 62%.

The preparation method of skin matrix, be the same as Example 4, and make accommodation.

Comparative example 2

A kind of skin matrix, it is main composed of the following components according to mass percentage：Propane diols 20%, pentanediol 5%, Flos Buddlejae extract 7%, tetrahydrochysene methylpyrimidine carboxylic acid 0%, gentian root extract 10%, water 58%.

Comparative example 3

A kind of skin matrix, it is main composed of the following components according to mass percentage：Propane diols 20%, pentanediol 5%, Flos Buddlejae extract 7%, tetrahydrochysene methylpyrimidine carboxylic acid 3%, gentian root extract 0%, water 65%.

Embodiment 7

A kind of emulsion, it is main composed of the following components according to mass percentage：

Component A：Methyl sesquistearate 1%, PEG-20 methyls sesquistearate 1%, glyceryl stearate Acid esters/PEG-100 stearates 1%, caprylic/capric triglyceride 5%, dimethyl silicone polymer 2%, docosyl alcohol 0.5%, carbon Dioctyl phthalate 5%, Nipasol 0.05%；

B component：Glycerine 5%, butanediol 5%, erythrite 1%, Sodium Hyaluronate 0.03%, allantoin 0.4%, carbomer 0.1%, EDETATE SODIUM 0.05%, water 52.86%；

Component C：Triethanolamine 0.5%；

D components：Skin matrix 20% made from embodiment 1, essence 0.01%；

The preparation method of emulsion comprises the following steps：

(1) component A is sequentially added to open after oil phase pot and stirred, mixing speed is 800r/min, and is warming up to 90 DEG C, so After be incubated 12min, obtain oil-phase component standby；

(2) B component is added to open after aqueous phase pot and stirred, mixing speed is 800r/min, and is warming up to 90 DEG C, Ran Houbao Warm 16min, obtains water-phase component standby；

(3) it will open and stir after water-phase component suction emulsification pot, mixing speed is 400r/min, then slow by oil-phase component Suction emulsification pot, then keeps vacuum -0.04MPa and mixing speed constant, opens outer circulation homogenizer and carries out homogeneous, The matter time is 3min, and homogeneous speed is 2000r/min；

(4) cooling water temperature is opened to component C is added after 58 DEG C, is then turned on outer circulation homogenizer and is carried out homogeneous, The matter time is 5min, and homogeneous speed is 3000r/min；

(5) continue to be cooled to addition D components after 48 DEG C, then stir 3min, mixing speed is 400r/min, be then turned on interior Recycle unit homogeneous 2min, homogeneous speed is 3000r/min, prepares emulsion.

Embodiment 8

Component A：Methyl sesquistearate 2%, PEG-20 methyls sesquistearate 0.5%, glycerine is hard Resin acid ester/PEG-100 stearates 2%, caprylic/capric triglyceride 2%, dimethyl silicone polymer 5%, docosyl alcohol 2%, carbon Dioctyl phthalate 3%, Nipasol 2%；

B component：Glycerine 10%, butanediol 2%, erythrite 3%, Sodium Hyaluronate 0.01%, allantoin 0.1%, card ripple Nurse 0.5%, EDETATE SODIUM 0.01%, water 35.75%；

Component C：Triethanolamine 0.1%；

D components：Skin matrix 30% made from embodiment 2, essence 0.03%；

The preparation method of emulsion comprises the following steps：

(1) component A is sequentially added to open after oil phase pot and stirred, mixing speed is 900r/min, and is warming up to 88 DEG C, so After be incubated 10min, obtain oil-phase component standby；

(2) B component is added to open after aqueous phase pot and stirred, mixing speed is 900r/min, and is warming up to 88 DEG C, Ran Houbao Warm 18min, obtains water-phase component standby；

(3) it will open and stir after water-phase component suction emulsification pot, mixing speed is 500r/min, then slow by oil-phase component Suction emulsification pot, then keeps vacuum -0.06MPa and mixing speed constant, opens outer circulation homogenizer and carries out homogeneous, The matter time is 4min, and homogeneous speed is 2500r/min；

(4) cooling water temperature is opened to component C is added after 52 DEG C, is then turned on outer circulation homogenizer and is carried out homogeneous, The matter time is 4min, and homogeneous speed is 2500r/min；

(5) continue to be cooled to addition D components after 48 DEG C, then stir 3min, mixing speed is 400r/min, be then turned on interior Recycle unit homogeneous 3min, homogeneous speed is 2500r/min, prepares emulsion.

Embodiment 9

Component A：Methyl sesquistearate 1.5%, PEG-20 methyls sesquistearate 0.8%, glycerine Stearate/PEG-100 stearates 1.5%, caprylic/capric triglyceride 4%, dimethyl silicone polymer 3%, docosyl alcohol 0.8%, carbonic acid dioctyl ester 4%, Nipasol 0.1%；

B component：Glycerine 8%, butanediol 4%, erythrite 2%, Sodium Hyaluronate 0.02%, allantoin 0.2%, carbomer 0.15%, EDETATE SODIUM 0.02%, water 39.74%；

Component C：Triethanolamine 0.15%；

D components：Skin matrix 30% made from embodiment 3, essence 0.02%；

The preparation method of emulsion comprises the following steps：

(1) component A is sequentially added to open after oil phase pot and stirred, mixing speed is 1000r/min, and is warming up to 85 DEG C, Then 10min is incubated, oil-phase component is obtained standby；

(2) B component is added to open after aqueous phase pot and stirred, mixing speed is 1000r/min, and is warming up to 85 DEG C, then 20min is incubated, water-phase component is obtained standby；

(3) it will open and stir after water-phase component suction emulsification pot, mixing speed is 600r/min, then slow by oil-phase component Suction emulsification pot, then keeps vacuum -0.05MPa and mixing speed constant, opens outer circulation homogenizer and carries out homogeneous, The matter time is 5min, and homogeneous speed is 3000r/min；

(4) cooling water temperature is opened to component C is added after 55 DEG C, is then turned on outer circulation homogenizer and is carried out homogeneous, The matter time is 3min, and homogeneous speed is 2000r/min；

(5) continue to be cooled to addition D components after 45 DEG C, then stir 5min, mixing speed is 600r/min, be then turned on interior Recycle unit homogeneous 1min, homogeneous speed is 2000r/min, prepares emulsion.

Embodiment 10

A kind of emulsion, according to mass percentage, except the skin matrix in D components is skin matrix made from embodiment 4 25%, other equal be the same as Examples 9 of component and content.

The preparation method be the same as Example 9 of emulsion.

Embodiment 11

A kind of emulsion, according to mass percentage, except the skin matrix in D components is skin matrix made from embodiment 5 30%, other equal be the same as Examples 9 of component and content.

The preparation method be the same as Example 9 of emulsion.

Embodiment 12

A kind of emulsion, according to mass percentage, except the skin matrix in D components is skin matrix made from embodiment 6 30%, other equal be the same as Examples 9 of component and content.

The preparation method be the same as Example 9 of emulsion.

Comparative example 4

A kind of emulsion, according to mass percentage, except the skin matrix in D components is skin matrix made from comparative example 1 30%, other equal be the same as Examples 9 of component and content.

The preparation method be the same as Example 9 of emulsion.

Comparative example 5

A kind of emulsion, according to mass percentage, except the skin matrix in D components is skin matrix made from comparative example 2 30%, other equal be the same as Examples 9 of component and content.

The preparation method be the same as Example 9 of emulsion.

Comparative example 6

A kind of emulsion, according to mass percentage, except the skin matrix in D components is skin matrix made from comparative example 3 30%, other equal be the same as Examples 9 of component and content.

The preparation method be the same as Example 9 of emulsion.

Experimental design and interpretation of result

To the various embodiments described above and comparative example while the efficiency analysis for light injury of putting up a resistance, in cell culture, is added The mass concentration of thing to be detected is set to 0%, 20% and 30% three kind and tested.

1) horn cell is protected

Human keratinous cell is cultivated, it is handled with the material that determinand is embodiment 1-12 and comparative example 1-6, culture 5h, is then irradiated with infrared ray, visible ray and ultraviolet, the survival rate of horn cell is determined after 3h, as a result as shown in table 1.

Survival rate of the horn cell of table 1 after the processing of various concentrations determinand

It can be seen from the result of table 1 compared with comparative example 1-3, skin matrix processing cutin is thin made from embodiment 1-6 After born of the same parents, the survival rate of horn cell is significantly improved, and wherein the effect of embodiment 3 is best, and within the specific limits, cell culture The concentration of Shi Suoyong skin matrix is higher, better to the protecting effect of horn cell；Compared with comparative example 4-6, embodiment 7- After emulsion processing horn cell made from 12, the survival rate of horn cell is also significantly improved, and wherein the effect of embodiment 9 is best, It is better to the protecting effect of horn cell and within the specific limits, the concentration of emulsion used during cell culture is higher.Explanation Skin matrix of the present invention and cosmetics (emulsion) including skin matrix of the present invention can effectively protect cells from light injury.

Wherein, do not include not including tetrahydrochysene methylpyrimidine carboxylic acid, contrast in Flos Buddlejae extract, comparative example 2 in comparative example 1 Do not include gentian root extract in example 3, compared with comparative example 1-3, embodiment 4 includes Flos Buddlejae extract, tetrahydrochysene methyl Pyrimidine carboxylic and gentian root extract, therefore the effect for protecting cells from light injury is more preferably, illustrates skin matrix of the present invention Middle each component has the effect mutually cooperateed with terms of light injury is protected cells from, and synergy is more preferably, includes simultaneously The cosmetics of skin matrix of the present invention also have the effect for protecting cells from light injury accordingly.

2) Langerhans cell is protected

Langerhans cell is cultivated, it is handled with the material that determinand is embodiment 1-12 and comparative example 1-6, culture 5h, is then irradiated with infrared ray, visible ray and ultraviolet, the survival rate of Langerhans cell is determined after 3h, as a result such as the institute of table 2 Show.

Survival rate of the Langerhans cell of table 2 after the processing of various concentrations determinand

It can be seen from the result of table 2 compared with comparative example 1-3, skin matrix made from embodiment 1-6 handles Lang Gehan After this cell, the survival rate of Langerhans cell is significantly improved, and wherein the effect of embodiment 3 is best, and within the specific limits, The concentration of skin matrix used is higher during cell culture, better to the protecting effect of Langerhans cell；With comparative example 4-6 phases Than, after emulsion processing Langerhans cell made from embodiment 7-12, the survival rate of Langerhans cell is also significantly improved, wherein The effect of embodiment 9 is best, and within the specific limits, the concentration of emulsion used is higher during cell culture, to Langerhans The protecting effect of cell is better.Illustrate that skin matrix of the present invention and the cosmetics (emulsion) including skin matrix of the present invention can Effectively protect cells from light injury.

3) prevent matrix metalloproteinase (MMP-1) from discharging

Various types of radiation can all cause the degraded of extracellular matrix, using the skin of in vitro culture, be with determinand Embodiment 1-12 and comparative example 1-6 material processing, are then irradiated with UVB, MMP-1 content are determined after 24h, as a result such as table 3 It is shown.

Burst size (units of the table 3MMP-1 after the processing of various concentrations determinand：pg/mL)

Group	Determinand is originated	0.00% determinand	20% determinand	30% determinand
					1	Embodiment 1	199000	135300	112300
2	Embodiment 2	199000	132000	109000
					3	Embodiment 3	199000	120500	100600
4	Embodiment 4	199000	135200	109800
					5	Embodiment 5	199000	134000	111400
6	Embodiment 6	199000	134800	109700
					7	Comparative example 1	199000	140300	118500
8	Comparative example 2	199000	138500	117100
					9	Comparative example 3	199000	136200	115300
10	Embodiment 7	199000	136500	114500
					11	Embodiment 8	199000	134400	112000
12	Embodiment 9	199000	124300	105600
					13	Embodiment 10	199000	136400	110800
14	Embodiment 11	199000	135200	116400
					15	Embodiment 12	199000	135100	109900
16	Comparative example 4	199000	141000	119700
					17	Comparative example 5	199000	139400	120100
18	Comparative example 6	199000	140200	119600

It can be seen from the result of table 3 compared with comparative example 1-3, the external training of skin matrix processing made from embodiment 1-6 After foster skin, MMP-1 expression can be significantly reduced, so as to reduce degradeds of the MMP-1 to collagen, wherein embodiment 3 Effect preferably, and within the specific limits, the concentration of skin matrix used is higher during in vitro culture skin, reduces MMP-1 The effect of expression is better；Compared with comparative example 4-6, after the skin of emulsion processing in vitro culture made from embodiment 7-12, MMP-1 Expression be significantly reduced, wherein the effect of embodiment 9 is preferably, and within the specific limits, used during in vitro culture skin The concentration of emulsion is higher, and the effect of reduction MMP-1 expression is better.Illustrate skin matrix of the present invention and including skin matrix of the present invention Cosmetics (emulsion) can effectively protect cells from light injury.

4) oxidative damage is prevented

Human keratinous cell is cultivated, it is handled with the material that determinand is embodiment 1-12 and comparative example 1-6, then made With radiation of visible light horn cell 20min, active oxygen (ROS) content (unit of accumulation is determined：RFU, Relative fluorescence units), knot Fruit is as shown in table 4.

Intracellular ROS content (unit after the processing of the various concentrations determinand of table 4：RFU)

Group	Determinand is originated	0.00% determinand	20% determinand	30% determinand
					1	Embodiment 1	248	169	151
2	Embodiment 2	248	161	148
					3	Embodiment 3	248	158	145
4	Embodiment 4	248	163	149
					5	Embodiment 5	248	160	148
6	Embodiment 6	248	162	150
					7	Comparative example 1	248	175	160
8	Comparative example 2	248	178	164
					9	Comparative example 3	248	174	163
10	Embodiment 7	248	171	154
					11	Embodiment 8	248	163	152
12	Embodiment 9	248	161	148
					13	Embodiment 10	248	166	150
14	Embodiment 11	248	164	153
					15	Embodiment 12	248	163	154
16	Comparative example 4	248	179	164
					17	Comparative example 5	248	182	167
18	Comparative example 6	248	178	166

It can be seen from the result of table 4 compared with comparative example 1-3, skin matrix processing cutin is thin made from embodiment 1-6 After born of the same parents, the ROS as caused by radiation of visible light can be substantially reduced and produced, the oxidation pressure that visible ray is brought effectively is protected cells from Power and damage, the wherein effect of embodiment 3 are best, and within the specific limits, skin matrix used is dense during cell culture Degree is higher, better to the protecting effect of horn cell；Compared with comparative example 4-6, emulsion made from embodiment 7-12 handles cutin After cell, it can also substantially reduce the ROS as caused by radiation of visible light and produce, wherein the effect of embodiment 9 is best, and one Determine in scope, the concentration of emulsion used is higher during cell culture, it is better to the protecting effect of horn cell.Illustrate present invention shield Skin matrix and cosmetics (emulsion) including skin matrix of the present invention can effectively protect cells from light injury.

5) thermal protection

Cultivate human keratinous cell, add the material culture 5h that determinand is embodiment 1-12 and comparative example 1-6, then 44 DEG C heat shock processing 30min, determines the content (unit of the lactic dehydrogenase (LDH) of the cell release of damaged membrane：RFU, phase To flat fluorescent；Weighed by the way that resazurin is converted into the result of the test of fluorescence resorufin), to determine the survival rate of cell. As a result it is as shown in table 5.

Content (the unit for the LDH that cell discharges after the processing of the various concentrations determinand of table 5：RFU)

As seen from the results in Table 5, compared with comparative example 1-3, after skin matrix processing horn cell made from embodiment 1-6, Cell death amount is obvious caused by the infringement that excessive infra-red radiation is brought, infrared ray and heat shock can effectively be protected cells from Less, the effect of wherein embodiment 3 is best, and within the specific limits, the concentration of skin matrix used is got over during cell culture Height is better to the protecting effect of horn cell；Compared with comparative example 4-6, emulsion made from embodiment 7-12 handles horn cell Afterwards, the cell damage situation as caused by heat shock can be also substantially reduced, therefore LDH content is relatively low, wherein the effect of embodiment 9 Fruit is best, and within the specific limits, the concentration of emulsion used is higher during cell culture, and the protecting effect of horn cell is got over It is good.Illustrate that skin matrix of the present invention and the cosmetics (emulsion) including skin matrix of the present invention can be protected cells from effectively Light injury.

Although illustrate and describing the present invention with specific embodiment, but it will be appreciated that without departing substantially from the present invention's In the case of spirit and scope, many other changes and modification can be made.It is, therefore, intended that in the following claims Including belonging to all such changes and modifications in the scope of the invention.

Claims

1. a kind of skin matrix, it is characterised in that according to mass percentage, main composed of the following components：

Propane diols 10-20%, pentanediol 3-5%, Flos Buddlejae extract 5-10%, tetrahydrochysene methylpyrimidine carboxylic acid 1-5%, gentian Root extract 5-10%, surplus is water.

2. skin matrix according to claim 1, it is characterised in that according to mass percentage, mainly by following components Composition：Propane diols 12-20%, pentanediol 4-5%, Flos Buddlejae extract 6-10%, tetrahydrochysene methylpyrimidine carboxylic acid 2-5%, Huanglong Courage root extract 6-10%, surplus is water；

Preferably, according to mass percentage, the skin matrix is mainly composed of the following components：Propane diols 15-20%, penta 2 Alcohol 4-5%, Flos Buddlejae extract 6-9%, tetrahydrochysene methylpyrimidine carboxylic acid 2-4%, gentian root extract 7-9%, surplus is water.

3. the preparation method of skin matrix described in a kind of claim 1 or 2, it is characterised in that comprise the following steps：

By the water of formula ratio, propane diols and pentanediol Hybrid Heating, Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic are added after cooling Acid and gentian root extract are uniformly mixed so as to obtain skin matrix.

4. preparation method according to claim 3, it is characterised in that temperature after heating is 85-90 DEG C；And/or, after cooling Temperature is 35-40 DEG C.

5. the preparation method according to claim 3 or 4, it is characterised in that the preparation method of the skin matrix, including such as Lower step：

(b) Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid and gentian root extract are added after cooling, separates, obtains after stirring To skin matrix.

6. preparation method according to claim 5, it is characterised in that in step (a), temperature after heating is 85-90 DEG C； And/or, mixing speed is 300-400r/min；And/or, soaking time is 20-30min；And/or, in step (b), after cooling Temperature is 35-40 DEG C；And/or, mixing speed is 800-1000r/min, and mixing time is 10-15min；And/or, separation is used The mode of filtering, filtering mesh number is 150-250 mesh；

(a) water, propane diols and pentanediol of formula ratio are mixed and heated to 85-90 DEG C, mixing speed is 300-400r/min, protected Warm 20-30min, obtains clear solution；

(b) it is cooled to after 35-40 DEG C and adds Flos Buddlejae extract, tetrahydrochysene methylpyrimidine carboxylic acid and the stirring of gentian root extract, Mixing speed is 800-1000r/min, and mixing time is 10-15min, then with 150-200 mesh filter-cloth filterings, obtains skin care base Matter.

7. application of the skin matrix described in claim 1 or 2 in cosmetics are prepared.

8. a kind of cosmetics, it is characterised in that including the skin matrix described in claim 1 or 2；

Preferably, the cosmetics include emulsion, cream, toner, Essence, muffin or sunscreen productses；

Preferably, mass percent of the skin matrix in cosmetics is 20-30%.

9. cosmetics according to claim 8, it is characterised in that according to mass percentage, mainly by following components group Into：

Component A：Methyl sesquistearate 1-2%, PEG-20 methyl sesquistearate 0.5-1%, glycerine is hard Resin acid ester/PEG-100 stearate 1-2%, caprylic/capric triglyceride 2-5%, dimethyl silicone polymer 2-5%, docosyl alcohol 0.5-2%, carbonic acid dioctyl ester 3-5%, Nipasol 0.05-0.2%；

B component：Glycerine 5-10%, butanediol 2-5%, erythrite 1-3%, Sodium Hyaluronate 0.01-0.03%, allantoin 0.1- 0.4%, carbomer 0.1-0.5%, EDETATE SODIUM 0.01-0.05%, surplus is water；

Component C：Triethanolamine 0.1-0.5%；

D components：Essence optional the skin matrix 20-30% and 0.01-0.03%；

Component A：Methyl sesquistearate 1.5-2%, PEG-20 methyl sesquistearate 0.5-1%, glycerine Stearate/PEG-100 stearate 1-2%, caprylic/capric triglyceride 3-4%, dimethyl silicone polymer 3-5%, behenyl Alcohol 1-2%, carbonic acid dioctyl ester 3-5%, Nipasol 0.01-0.2%；

B component：Glycerine 6-9%, butanediol 3-5%, erythrite 1-3%, Sodium Hyaluronate 0.01-0.03%, allantoin 0.2- 0.4%, carbomer 0.2-0.5%, EDETATE SODIUM 0.02-0.05%, surplus is water；

Component C：Triethanolamine 0.2-0.5%；

D components：Essence optional the skin matrix 25-30% and 0.02-0.03%.

10. the preparation method of cosmetics described in a kind of claim 9, it is characterised in that comprise the following steps：It will be mixed by component A Even obtained oil-phase component carries out homogeneous with the water-phase component being uniformly mixed so as to obtain by B component, then adds component C homogeneous again, then add Cosmetics are obtained after entering D components and optional essence, homogeneous；

(a) by component A each material add oil phase pot, stirring be warming up to 85-90 DEG C, be then incubated it is standby, obtain oil phase into Point；

(b) by B component each material add aqueous phase pot, stirring be warming up to 85-90 DEG C, be then incubated it is standby, obtain aqueous phase into Point；

(c) oil-phase component suction emulsification pot is kept into vacuum and stirring speed again after water-phase component suction emulsification pot is stirred Spend constant, homogeneous 3-5min；

(d) component C is added after cooling, then carries out homogeneous 3-5min；

(e) continue to cool, stir 3-5min, homogeneous 1-3min after adding D components and optional essence, obtain cosmetics；

Preferably, in step (a), mixing speed is 800-1000r/min；And/or, soaking time is 10-15min；

Preferably, in step (b), mixing speed is 800-1000r/min；And/or, soaking time is 15-20min；

Preferably, in step (c), mixing speed is 400-600r/min；And/or, vacuum is -0.04~-0.06MPa；With/ Or, homogeneous speed is 2000-3000r/min；

Preferably, in step (d), temperature is 52-58 DEG C after cooling；And/or, homogeneous speed is 2000-3000r/min；

Preferably, in step (e), the temperature after cooling is 42-48 DEG C；And/or, mixing speed is 400-600r/min；With/ Or, homogeneous speed is 2000-3000r/min.