CN107271417A - A kind of biochip and its detector and system and its annotation mark and discrimination method - Google Patents
A kind of biochip and its detector and system and its annotation mark and discrimination method Download PDFInfo
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- CN107271417A CN107271417A CN201710560150.1A CN201710560150A CN107271417A CN 107271417 A CN107271417 A CN 107271417A CN 201710560150 A CN201710560150 A CN 201710560150A CN 107271417 A CN107271417 A CN 107271417A
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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Abstract
The invention discloses a kind of biochip and its detector and system of technical field of biological and its annotation mark and discrimination method, including biochip body, substrate is provided with the left of the surface of the biochip body, bar code is provided with the right side of the surface of the biochip body, this kind of biochip and its detector and system and its annotation mark and discrimination method, while fluorescent tag molecule and DNA chip hybridize can choose friends the detection of signal, it need not wash as hybrid molecule, simplify operating procedure, and PMT structure definitions are high, Detection results are good, and bar code is set on biochip body, logical laser code reader collection, it can recognize whether biochip is previously used, and then biochip can be changed in time, avoid influenceing testing result.
Description
Technical field
The present invention relates to technical field of biological, specially a kind of biochip and its detector and system and its note
Note mark and discrimination method.
Background technology
Biochip, also known as protein chip or genetic chip, they originate from DNA hybridization probe technique and semi-conductor industry
The crystallization that technology is combined.The technology mean by a large amount of probe molecules be fixed on holder after with the DNA with fluorescence labeling or
Other sample molecules (such as albumen, the factor or small molecule) are hybridized, by detecting that the hybridization signal of each probe molecule is strong
The quantity and sequence information of sample molecule are spent and then obtain, biochip is exactly in materials such as one piece of sheet glass, silicon chip, nylon membranes
On put biological sample, signal is then collected by a kind of instrument, with computer analyze data result, but biologies most at present
Chip detector is by CCD imaging detecting instruments, to be imaged low, and Detection results are poor, and current used biochip, due to by
Mistake may be reused, the pollution under being remained due to last use so that testing result is wrong, and existing detector is not
It can detect whether biochip had used, one kind that the new number of patent application CN201410380947.X of such as China is proposed is raw
Thing chip detector, is just CCD imaging detecting instruments, and can not detect whether biochip had used, it is therefore desirable to right
Current biochip test does the improvement through a step.
The content of the invention
It is an object of the invention to provide a kind of biochip and its detector and system and its annotation mark and identification
Method, is generally CCD imaging detecting instruments with the traditional detector for solving to propose in above-mentioned background technology, Detection results are poor, and
It can not detect whether biochip had used, the problem of influenceing testing result.
To achieve the above object, the present invention provides following technical scheme：A kind of biochip, including biochip body,
It is provided with the left of the surface of the biochip body on the right side of substrate, the surface of the biochip body and is provided with bar code.
A kind of biochip supplies analysis, including detector housing, the inner chamber of the detector housing be divided into collection chamber and
Analyze and be installed with laser group, the left side wall of the detector housing on the left of chamber, the inner cavity top of the collection chamber
It is plugged with the top of movable plate, the movable plate and is provided with groove, the inner chamber left side wall of the collection chamber is fixed respectively up and down is connected
Light path selector support frame and dichroscope are connected to, the right-hand member of the light path selector support frame is fixedly connected with light path selection
Vertical supporting plate is connected with the top of device, the dichroscope, the right-hand member of the vertical supporting plate is connected with optical filtering by support
Piece, the right side wall of the vertical supporting plate is connected with reception objective lens support, and the bottom of the reception objective lens support is fixedly connected with
Object lens are received, the right side wall of the reception objective lens support is connected with pinhole blind, the interior bottom of chamber of the collection chamber by support
Portion is fixedly connected with laser code reader support, and the left end of the laser code reader support is fixedly connected with laser code reader, described
The inner chamber right side wall of collection chamber is fixedly connected with PMT image acquisition devices, and the intracavity bottom of the analysis chamber is fixedly connected with
Main frame, the inner chamber right side wall of the analysis chamber is fixedly connected with driving detection means, and the inner cavity top of the analysis chamber is consolidated
Surely it is connected with siren.
It is preferred that, the optical filter, receive object lens, the center line of pinhole blind and PMT image acquisition devices be respectively positioned on it is same
On horizontal line.
A kind of biochip supplies analysis system, including main frame, laser scan code system, memory storage unit, driving detection system
System, warning system and biological chips detection system；
The main frame is used to receive data message, analyzing and processing data information and transmission instruction etc.；
The laser scan code system includes laser barcode scanning processor, laser data transmission unit, A/D converting units, signal
The information of bar code on amplifying unit and laser barcode scanning unit, the laser barcode scanning unit collection biochip body, and will
Signal is transferred to signal amplification unit, and the signal amplification unit amplifies signal, and the signal after amplification is transferred into A/D turns
Unit is changed, the A/D converting units convert analog signals into data signal and are sent to laser barcode scanning processor, and the laser is swept
Digital laser signal after processing is sent to laser data and passed by code processor to the digital laser signal analysis and processing of reception
Defeated unit, the laser data transmission unit is connected with main frame；
The memory storage unit stores laser intelligence data；
The driving detecting system includes driving measurement processor, host command receiving unit and multi-channel control circuit, main
Machine instruction reception unit Receiving Host is instructed, and host command is sent into driving measurement processor, the driving detection process
Device receives instruction, and sends instruction, the multi-channel control Current Controller circuit connection to multi-channel control circuit；
The warning system includes alarm processor, circuit for alarming on-unit, D/A converting units, audio amplification unit
And loudspeaker, the circuit for alarming on-unit connects circuit, and the alarm processor work, the alarm processor sends police
The number of notifying, and D/A converting units are transferred to, the D/A converting units, which are converted digital signals into, is sent to sound after analog signal
Frequency amplifying unit, the audio amplification unit is sent to loudspeaker after amplifying to audio；
The biological chips detection system includes biochip test processor, detection circuit receiving unit, analog-to-digital conversion
Unit, filter unit, PMT sensors, the detection circuit receiving unit are connected after circuit, and the biological chips detection system leads to
Electricity, the PMT sensors gather fluorescence spectrum image, and fluorescence spectrum image is sent into filter unit, the filter unit
Fluorescence spectrum image is made after filtering process, AD conversion unit is sent to, the AD conversion unit changes analog signal
Into data signal, biochip test processor, digital phosphor light of the biochip test processor to reception are sent to
After analysis of spectrum and processing, processing data is sent to display output unit, processing data is sent to by the display output unit
Display screen, the display screen display processing data.
A kind of biochip supplies analysis annotation mark and discrimination method, comprise the following steps：Biochip preparation of specimen, sweep
Code processing and fluorescence biosensor chip detection, this kind of biochip supplies analysis annotation mark and discrimination method, are comprised the following steps that：
S1：Biochip preparation of specimen：The biological sample for needing to detect is put into substrate on biochip body, it
Biochip body is put into the groove on movable plate afterwards, movable plate is promoted afterwards, movable plate is driven biochip sheet
Body is entered in acquisition cavity room；
S2：Barcode scanning processing：The information of bar code on the biochip body of laser barcode scanning unit collection afterwards, and by signal
Signal amplification unit is transferred to, signal amplification unit amplifies signal, and the signal after amplification is transferred to A/D converting units,
A/D converting units convert analog signals into data signal and are sent to laser barcode scanning processor, laser barcode scanning processor pair afterwards
The digital laser signal analysis and processing of reception, and the digital laser signal after processing is sent to laser data transmission unit, swash
Digital laser signal is sent to main frame by light data transmission unit, and main frame is analyzed and handled to digital laser signal, and from memory
The barcode laser digital information gathered before being obtained in memory cell, is contrasted, if the barcode laser numeral letter of collection
Breath it is identical with the one of barcode laser digital information stored up from memory storage element memory, then main frame to drive detecting system
Interior host command receiving unit sends alarm signal, and alarm signal is sent to driving detection process by host command receiving unit
After device, the analysis of driving measurement processor and processing alarm signal, and instruction is sent to multi-channel control circuit, multi-channel control circuit connects
Logical warning system, warning system sends alarm, reminds the biochip to use, it is necessary to change biochip, if collection
Barcode laser digital information and from memory storage element memory store up one barcode laser digital information of any of which all
Difference, then host command receiving unit of the main frame into driving detecting system, which is sent, detects connection instruction, and host command receives single
Detection connection instruction is sent to driving measurement processor by member, and driving measurement processor analysis is connected with processing detection after instruction,
And instruction is sent to multi-channel control circuit, make multi-channel control circuit communication biological chips detection system, start fluoroscopic examination；
S3：Fluorescence biosensor chip is detected：Start laser group, laser group transmitting laser, afterwards multigroup laser beam entrance
Light path selection is carried out in light path selector, the single beam laser after light path selector is selected passes through dichroscope afterwards, shone
The biological sample on substrate is mapped to, the light on biological sample is transferred in optical filter after dichroscope reflects afterwards, afterwards
Fluorescence after the refraction for receiving object lens, is passed from the loophole on pinhole blind again, the fluorescence of PTM image acquisition devices collection afterwards
Fluorescence spectrum image is sent to filter unit by the PMT sensors in spectrum, PTM image acquisition devices, and filter unit is to fluorescence light
Spectrogram picture is made after filtering process, is sent to AD conversion unit, and AD conversion unit converts analog signals into data signal, hair
Biochip test processor is given, will after biochip test processor is to the digital phosphor spectrum analysis and processing of reception
Processing data is sent to display output unit, and processing data is sent to display screen, display screen display processing by display output unit
Data.
It is preferred that, in the step S1, the length of groove is more than the width of biochip body.
Compared with prior art, the beneficial effects of the invention are as follows：This kind of biochip and its detector and system and its
Annotation is marked and discrimination method, while fluorescent tag molecule and DNA chip hybridize can choose friends the detection of signal, nothing
It need to wash as hybrid molecule, simplify operating procedure, and PMT structure definitions are high, and Detection results are good, and in biological core
Bar code is set on piece body, leads to the collection of laser code reader, can recognize whether biochip is previously used, and then can be timely
Change biochip, it is to avoid influence testing result.
Brief description of the drawings
Fig. 1 is schematic structural view of the invention；
Fig. 2 is detector structural representation of the present invention；
Fig. 3 is detector system principle diagram of the present invention；
Fig. 4 is laser scan code system block diagram of the present invention；
Fig. 5 is present invention driving detecting system block diagram；
Fig. 6 is warning system block diagram of the present invention；
Fig. 7 is biological chips detection system block diagram of the present invention.
In figure：1 biochip body, 2 substrates, 3 bar codes, 4 detector housings, 5 collection chambers, 6 analysis cavities
Room, 7 laser groups, 8 movable plates, 9 grooves, 10 light path selector support frames, 11 dichroscopes, 12 light path selectors,
13 vertical supporting plates, 14 optical filters, 15 receive objective lens support, 16 reception object lens, 17 pinhole blinds, 18 laser code readers
Support, 19 laser code readers, 20 PMT image acquisition devices, 21 main frames, 22 driving detection means, 23 sirens, 24 laser
Scan code system, 241 laser barcode scanning processors, 242 laser data transmission units, 243 A/D converting units, the amplification of 244 signals
Unit, 245 laser barcode scanning units, 25 memory storage units, 26 driving detecting systems, 261 driving measurement processors, 262
Host command receiving unit, 263 multi-channel control circuits, 27 warning systems, 271 alarm processors, 272 circuit for alarming are connected
Unit, 273 D/A converting units, 274 audio amplification units, 275 loudspeakers, 28 biological chips detection systems, 281 biologies
Chip measurement processor, 282 detection circuit receiving units, 283 AD conversion units, 284 filter units, 285 PMT sensings
Device, 286 display output units, 287 display screens.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Fig. 1-7 are referred to, the present invention provides a kind of technical scheme：A kind of biochip, including biochip body 1, institute
It is provided with the left of the surface for stating biochip body 1 on the right side of substrate 2, the surface of the biochip body 1 and is provided with bar code
3。
The present invention also provides a kind of biochip supplies analysis, including detector housing 4, the inner chamber of the detector housing 4
It is divided on the left of collection chamber 5 and analysis chamber 6, the inner cavity top of the collection chamber 5 and is installed with laser group 7, it is described
The left side wall of detector housing 4 is plugged with movable plate 8, and the top of the movable plate 8 is provided with groove 9, described to gather the interior of chamber 5
Light path selector support frame 10 and dichroscope 11, the light path selector support frame have been respectively fixedly connected with above and below chamber left side wall
10 right-hand member is fixedly connected with light path selector 12, and the top of the dichroscope 11 is connected with vertical supporting plate 13, described perpendicular
Optical filter 14 is connected with by support to the right-hand member of supporting plate 13, the right side wall of the vertical supporting plate 13 is connected with reception object lens
Support 15, the bottom for receiving objective lens support 15 is fixedly connected with reception object lens 16, the right side for receiving objective lens support 15
Wall is connected with pinhole blind 17 by support, and the intracavity bottom of the collection chamber 5 is fixedly connected with laser code reader support 18,
The left end of the laser code reader support 18 is fixedly connected with laser code reader 19, and the inner chamber right side wall of the collection chamber 5 is consolidated
Surely PMT image acquisition devices 20 are connected with, the intracavity bottom of the analysis chamber 6 is fixedly connected with main frame 21, the analysis chamber 6
Inner chamber right side wall be fixedly connected with driving detection means 22, it is described analysis chamber 6 inner cavity top be fixedly connected with siren
23, the optical filter 14, receive object lens 16, the center line of pinhole blind 17 and PMT image acquisition devices 20 is respectively positioned on same level
On line.
The present invention provides a kind of biochip supplies analysis system, including main frame 21, laser scan code system 24, memory storage again
Unit 25, driving detecting system 26, warning system 27 and biological chips detection system 28；
The main frame 21 is used to receive data message, analyzing and processing data information and transmission instruction etc.；
The laser scan code system 24 includes laser barcode scanning processor 241, laser data transmission unit 242, A/D conversion lists
On member 243, signal amplification unit 244 and laser barcode scanning unit 245, the collection biochip of laser barcode scanning unit 245 body 1
Bar code 3 information, and transmit a signal to signal amplification unit 244, the signal amplification unit 244 amplifies signal,
And the signal after amplification is transferred to A/D converting units 243, the A/D converting units 243 convert analog signals into digital letter
Number it is sent to laser barcode scanning processor 241, the digital laser signal analysis and processing of 241 pairs of receptions of laser barcode scanning processor,
And the digital laser signal after processing is sent to laser data transmission unit 242, the laser data transmission unit 242 and master
Machine 1 is connected；
The memory storage unit 25 stores laser intelligence data；
The driving detecting system 26 includes driving measurement processor 261, host command receiving unit 262 and multi-channel control
Circuit 263, the instruction of the Receiving Host of host command receiving unit 262, and host command is sent to driving measurement processor, it is described
Drive measurement processor 261 to receive instruction, and instruction is sent to multi-channel control circuit 263, the multi-channel control circuit 263 is controlled
Circuit communication；
The warning system 27 includes alarm processor 271, circuit for alarming on-unit 272, D/A converting units 273, sound
Frequency amplifying unit 274 and loudspeaker 275, the circuit for alarming on-unit 272 connect circuit, the work of alarm processor 271
Make, the signal an alert of alarm processor 271, and be transferred to D/A converting units 273, the D/A converting units 273 will
Data signal is converted into being sent to audio amplification unit 274 after analog signal, after the audio amplification unit 274 amplifies to audio
It is sent to loudspeaker 275；
The biological chips detection system 28 include biochip test processor 281, detection circuit receiving unit 282,
AD conversion unit 283, filter unit 284, PMT sensors 285, the detection circuit receiving unit 282 are connected after circuit, institute
State biological chips detection system 28 to be powered, the PMT sensors 285 gather fluorescence spectrum image, and fluorescence spectrum image is sent out
Filter unit 284 is given, the filter unit 284 is made after filtering process to fluorescence spectrum image, is sent to AD conversion unit
283, the AD conversion unit 283 converts analog signals into data signal, is sent to biochip test processor 281,
After the digital phosphor spectrum analysis and processing of described 281 pairs of receptions of biochip test processor, processing data is sent to aobvious
Show output unit 286, processing data is sent to display screen 287 by the display output unit 286, and the display screen 287 is shown
Processing data.
The present invention provides a kind of biochip supplies analysis annotation mark and discrimination method again, comprises the following steps：Biological core
Piece preparation of specimen, barcode scanning processing and fluorescence biosensor chip detection, this kind of biochip supplies analysis annotation mark and discrimination method, tool
Body step is as follows：
S1：Biochip preparation of specimen：The biological sample for needing to detect is put into substrate 2 on biochip body 1,
Biochip body 1 is put into the groove 9 on movable plate 8 afterwards, movable plate 8 is promoted afterwards, movable plate 8 is driven biology
Chip body 1 is entered in collection chamber 5；
S2：Barcode scanning processing：Laser barcode scanning unit 245 gathers the information of the bar code 3 on biochip body 1 afterwards, and
Signal amplification unit 244 is transmitted a signal to, signal amplification unit 244 amplifies signal, and the signal after amplification is transferred to
A/D converting units 243, afterwards A/D converting units 243 convert analog signals into data signal and be sent to laser barcode scanning processor
241, the digital laser signal analysis and processing of 241 pairs of receptions of laser barcode scanning processor, and the digital laser signal after processing is sent out
Laser data transmission unit 242 is given, digital laser signal is sent to main frame 21, main frame 21 by laser data transmission unit 242
Digital laser signal is analyzed and handled, and the barcode laser numeral letter gathered out of memory storage unit 25 before acquisition
Breath, is contrasted, if the barcode laser digital information of collection and one of bar shaped from the memory storage of memory storage unit 25
Code laser digital information is identical, then host command receiving unit 262 of the main frame 21 into driving detecting system 26 sends alarm signal
Number, alarm signal is sent to driving measurement processor 261 by host command receiving unit 262, and driving measurement processor 261 is analyzed
Instruction is sent after processing alarm signal, and to multi-channel control circuit 263, the connection warning system 27 of multi-channel control circuit 263 is alert
Reporting system 27 sends alarm, reminds the biochip to use, it is necessary to change biochip, if the barcode laser of collection
Digital information is all different from one barcode laser digital information of any of which from the memory storage of memory storage unit 25, then main frame
The 21 host command receiving unit 262 into driving detecting system 26 sends detection connection instruction, host command receiving unit 262
Detection connection instruction is sent to driving measurement processor 261, the driving analysis of measurement processor 261 connects instruction with processing detection
Afterwards, and send and instruct to multi-channel control circuit 263, multi-channel control circuit 263 is connected biological chips detection system 28, start glimmering
Light is detected；
S3：Fluorescence biosensor chip is detected：Start laser group 7, laser group 7 launches laser, and multigroup laser beam enters afterwards
Enter and light path selection is carried out into light path selector 12, the single beam laser after the selection of light path selector 12 passes through dichroic afterwards
Mirror 11, is irradiated to the biological sample on substrate 2, and the light on biological sample is transferred to optical filtering after the reflection of dichroscope 11 afterwards
In piece 14, fluorescence is passed again after receiving the refraction of object lens 16 from the loophole on pinhole blind 17 afterwards, and PTM schemes afterwards
Fluorescence spectrum image is sent to filter as collector 20 gathers the PMT sensors 285 in fluorescence spectrum, PTM image acquisition devices 20
Ripple unit 284, filter unit 284 is made after filtering process to fluorescence spectrum image, is sent to AD conversion unit 283, and modulus turns
Change unit 283 and convert analog signals into data signal, be sent at biochip test processor 281, biochip test
After the digital phosphor spectrum analysis and processing of managing 281 pairs of receptions of device, processing data is sent to display output unit 286, shown
Processing data is sent in display screen 287, the display processing data of display screen 287, the step S1 by output unit 286, groove 9
Length be more than biochip body 1 width.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of biochip, including biochip body (1), it is characterised in that：The surface of the biochip body (1) is left
Side is provided with the right side of substrate (2), the surface of the biochip body (1) and is provided with bar code (3).
2. a kind of biochip supplies analysis, including detector housing (4), it is characterised in that：The inner chamber of the detector housing (4)
It is divided on the left of collection chamber (5) and analysis chamber (6), the inner cavity top of the collection chamber (5) and is installed with laser group
(7), the left side wall of the detector housing (4) is plugged with the top of movable plate (8), the movable plate (8) and is provided with groove (9),
Light path selector support frame (10) and dichroscope have been respectively fixedly connected with above and below the inner chamber left side wall of the collection chamber (5)
(11), the right-hand member of the light path selector support frame (10) is fixedly connected with light path selector (12), the dichroscope (11)
Top be connected with vertical supporting plate (13), the right-hand member of the vertical supporting plate (13) is connected with optical filter (14) by support,
The right side wall of the vertical supporting plate (13) is connected with reception objective lens support (15), and the bottom for receiving objective lens support (15) is consolidated
Surely reception object lens (16) are connected with, the right side wall for receiving objective lens support (15) is connected with pinhole blind (17) by support,
The intracavity bottom of the collection chamber (5) is fixedly connected with laser code reader support (18), the laser code reader support (18)
Left end be fixedly connected with laser code reader (19), the inner chamber right side wall of the collection chamber (5) is fixedly connected with PMT images and adopted
Storage (20), the intracavity bottom of the analysis chamber (6) is fixedly connected with main frame (21), and the inner chamber of the analysis chamber (6) is right
Side wall is fixedly connected with driving detection means (22), and the inner cavity top of the analysis chamber (6) is fixedly connected with siren (23).
3. a kind of biochip supplies analysis according to claim 2, it is characterised in that：The optical filter (14), reception thing
The center line of mirror (16), pinhole blind (17) and PMT image acquisition devices (20) is respectively positioned in same horizontal line.
4. a kind of biochip supplies analysis system, it is characterised in that：Deposited including main frame (21), laser scan code system (24), memory
Storage unit (25), driving detecting system (26), warning system (27) and biological chips detection system (28)；
The main frame (21) is used to receive data message, analyzing and processing data information and transmission instruction etc.；
The laser scan code system (24) includes laser barcode scanning processor (241), laser data transmission unit (242), A/D conversions
Unit (243), signal amplification unit (244) and laser barcode scanning unit (245), laser barcode scanning unit (245) collection are biological
The information of bar code (3) on chip body (1), and signal amplification unit (244) is transmitted a signal to, the signal amplification is single
First (244) amplify signal, and the signal after amplification is transferred into A/D converting units (243), the A/D converting units (243)
Convert analog signals into data signal and be sent to laser barcode scanning processor (241), laser barcode scanning processor (241) docking
The digital laser signal analysis and processing of receipts, and the digital laser signal after processing is sent to laser data transmission unit (242),
The laser data transmission unit (242) is connected with main frame (21)；
The memory storage unit (25) stores laser intelligence data；
The driving detecting system (26) includes driving measurement processor (261), host command receiving unit (262) and multichannel control
Circuit (263) processed, the instruction of host command receiving unit (262) Receiving Host, and host command is sent to driving detection process
Device, the driving measurement processor (261) receives instruction, and sends instruction, the multi-channel control to multi-channel control circuit (263)
Circuit (263) controls circuit communication；
The warning system (27) includes alarm processor (271), circuit for alarming on-unit (272), D/A converting units
(273), audio amplification unit (274) and loudspeaker (275), the circuit for alarming on-unit (272) connect circuit, the police
Processor (271) work, alarm processor (271) signal an alert are reported, and is transferred to D/A converting units (273), institute
State D/A converting units (273) and convert digital signals into after analog signal and be sent to audio amplification unit (274), the audio
Amplifying unit (274) is sent to loudspeaker (275) after amplifying to audio；
The biological chips detection system (28) includes biochip test processor (281), detection circuit receiving unit
(282), AD conversion unit (283), filter unit (284), PMT sensors (285), the detection circuit receiving unit
(282) connect after circuit, the biological chips detection system (28) is powered, the PMT sensors (285) gather fluorescence spectra
Picture, and fluorescence spectrum image is sent to filter unit (284), the filter unit (284) filters to fluorescence spectrum image
After processing, AD conversion unit (283) is sent to, the AD conversion unit (283) converts analog signals into data signal,
It is sent to biochip test processor (281), digital phosphor spectrum of the biochip test processor (281) to reception
After analysis and processing, processing data is sent to display output unit (286), the display output unit (286) will handle number
According to being sent to display screen (287), display screen (287) the display processing data.
5. a kind of biochip supplies analysis annotation mark and discrimination method, comprise the following steps：Biochip preparation of specimen, barcode scanning
Processing and fluorescence biosensor chip detection, it is characterised in that：This kind of biochip supplies analysis annotation mark and discrimination method, specific step
It is rapid as follows：
S1：Biochip preparation of specimen：The biological sample for needing to detect is put into substrate (2) on biochip body (1),
In the groove (9) that biochip body (1) is put on movable plate (8) afterwards, movable plate (8) is promoted afterwards, makes movable plate
(8) biochip body 1 is driven to enter in collection chamber (5)；
S2：Barcode scanning processing：Laser barcode scanning unit (245) gathers the information of the bar code (3) on biochip body (1) afterwards,
And signal amplification unit (244) is transmitted a signal to, signal amplification unit (244) amplifies signal, and by the signal after amplification
A/D converting units (243) are transferred to, A/D converting units (243) convert analog signals into data signal and are sent to laser afterwards
Barcode scanning processor (241), laser barcode scanning processor (241) is to the digital laser signal analysis and processing of reception, and by after processing
Digital laser signal is sent to laser data transmission unit (242), and laser data transmission unit (242) sends out digital laser signal
Main frame (21) is given, main frame (21) is analyzed and handled to digital laser signal, and out of memory storage unit (25) before acquisition
The barcode laser digital information of collection, is contrasted, if the barcode laser digital information of collection with from memory storage unit
(25) one of barcode laser digital information of memory storage is identical, then master of the main frame (21) into driving detecting system (26)
Machine instruction reception unit (262) sends alarm signal, and alarm signal is sent to driving detection by host command receiving unit (262)
After processor (261), driving measurement processor (261) analysis and processing alarm signal, and sent to multi-channel control circuit (263)
Instruction, multi-channel control circuit (263) connection warning system (27), warning system (27) sends alarm, has reminded the biochip
Through using crossing, it is necessary to change biochip, if the barcode laser digital information of collection with from memory storage unit (25) internal memory
One barcode laser digital information of any of which of storage is all different, then main frame of the main frame (21) into driving detecting system (26)
Instruction reception unit (262) sends detection connection instruction, and host command receiving unit (262) will detect that connection instruction is sent to drive
Dynamic measurement processor (261), driving measurement processor (261) analysis is connected with processing detection after instruction, and electric to multi-channel control
Road (263) sends instruction, multi-channel control circuit (263) is connected biological chips detection system (28), starts fluoroscopic examination；
S3：Fluorescence biosensor chip is detected：Start laser group (7), laser group (7) launches laser, and multigroup laser beam enters afterwards
Enter and light path selection is carried out into light path selector (12), the single beam laser after light path selector (12) selection passes through two afterwards
To Look mirror (11), the biological sample on substrate (2) is irradiated to, the light on biological sample is after dichroscope (11) reflection afterwards
It is transferred in optical filter (14), fluorescence is again after receiving the refraction of object lens (16) afterwards, the printing opacity from pinhole blind (17)
Hole is passed, the fluorescence spectrum of PTM image acquisition devices (20) collection afterwards, the PMT sensors (285) in PTM image acquisition devices (20)
Fluorescence spectrum image is sent to filter unit (284), filter unit (284) is made after filtering process to fluorescence spectrum image, sent out
AD conversion unit (283) is given, AD conversion unit (283) converts analog signals into data signal, is sent to biological core
Piece measurement processor (281), will after biochip test processor (281) is to the digital phosphor spectrum analysis and processing of reception
Processing data is sent to display output unit (286), and processing data is sent to display screen (287) by display output unit (286),
Display screen (287) display processing data.
6. a kind of biochip supplies analysis annotation mark according to claim 5 and discrimination method, it is characterised in that：It is described
In step S1, the length of groove (9) is more than the width of biochip body (1).
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