CN107267574A - A kind of Dendrobium officinale polysaccharide fragment and its extracting method - Google Patents
A kind of Dendrobium officinale polysaccharide fragment and its extracting method Download PDFInfo
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- CN107267574A CN107267574A CN201710527430.2A CN201710527430A CN107267574A CN 107267574 A CN107267574 A CN 107267574A CN 201710527430 A CN201710527430 A CN 201710527430A CN 107267574 A CN107267574 A CN 107267574A
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- dendrobium officinale
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- 150000004676 glycans Polymers 0.000 title claims abstract description 91
- 150000004804 polysaccharides Polymers 0.000 title claims abstract description 63
- 241001076416 Dendrobium tosaense Species 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 29
- 241000026010 Dendrobium candidum Species 0.000 claims abstract description 43
- 235000000346 sugar Nutrition 0.000 claims abstract description 20
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 15
- 230000004048 modification Effects 0.000 claims abstract description 15
- 238000012986 modification Methods 0.000 claims abstract description 15
- 238000003809 water extraction Methods 0.000 claims abstract description 11
- 239000000203 mixture Substances 0.000 claims abstract description 9
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000000605 extraction Methods 0.000 claims description 40
- 239000000284 extract Substances 0.000 claims description 33
- 239000000243 solution Substances 0.000 claims description 33
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 29
- 108090000790 Enzymes Proteins 0.000 claims description 28
- 102000004190 Enzymes Human genes 0.000 claims description 28
- 229940088598 enzyme Drugs 0.000 claims description 28
- 229920001282 polysaccharide Polymers 0.000 claims description 27
- 239000005017 polysaccharide Substances 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 27
- 239000003795 chemical substances by application Substances 0.000 claims description 24
- 108010059892 Cellulase Proteins 0.000 claims description 21
- 229940106157 cellulase Drugs 0.000 claims description 21
- 108010001817 Endo-1,4-beta Xylanases Proteins 0.000 claims description 20
- 239000006286 aqueous extract Substances 0.000 claims description 19
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 16
- 239000000047 product Substances 0.000 claims description 14
- 239000003153 chemical reaction reagent Substances 0.000 claims description 13
- 238000000502 dialysis Methods 0.000 claims description 10
- 239000013049 sediment Substances 0.000 claims description 10
- 239000006228 supernatant Substances 0.000 claims description 10
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 9
- 239000012535 impurity Substances 0.000 claims description 9
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 8
- 239000001569 carbon dioxide Substances 0.000 claims description 8
- 239000000287 crude extract Substances 0.000 claims description 8
- 238000001641 gel filtration chromatography Methods 0.000 claims description 7
- 238000007654 immersion Methods 0.000 claims description 7
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- 239000012507 Sephadex™ Substances 0.000 claims description 5
- GZCGUPFRVQAUEE-KVTDHHQDSA-N aldehydo-D-mannose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O GZCGUPFRVQAUEE-KVTDHHQDSA-N 0.000 claims description 5
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- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract description 22
- 239000008280 blood Substances 0.000 abstract description 20
- 210000004369 blood Anatomy 0.000 abstract description 20
- 241000700159 Rattus Species 0.000 abstract description 13
- 102000004877 Insulin Human genes 0.000 abstract description 11
- 108090001061 Insulin Proteins 0.000 abstract description 11
- 239000008103 glucose Substances 0.000 abstract description 11
- 229940125396 insulin Drugs 0.000 abstract description 11
- 230000002218 hypoglycaemic effect Effects 0.000 abstract description 8
- 229940127003 anti-diabetic drug Drugs 0.000 abstract description 6
- 239000003472 antidiabetic agent Substances 0.000 abstract description 6
- 238000002474 experimental method Methods 0.000 abstract description 5
- ZJJXGWJIGJFDTL-UHFFFAOYSA-N glipizide Chemical compound C1=NC(C)=CN=C1C(=O)NCCC1=CC=C(S(=O)(=O)NC(=O)NC2CCCCC2)C=C1 ZJJXGWJIGJFDTL-UHFFFAOYSA-N 0.000 abstract description 5
- 229960001381 glipizide Drugs 0.000 abstract description 5
- 201000001421 hyperglycemia Diseases 0.000 abstract description 5
- 210000000496 pancreas Anatomy 0.000 abstract description 5
- ZSJLQEPLLKMAKR-UHFFFAOYSA-N Streptozotocin Natural products O=NN(C)C(=O)NC1C(O)OC(CO)C(O)C1O ZSJLQEPLLKMAKR-UHFFFAOYSA-N 0.000 abstract description 4
- 230000009194 climbing Effects 0.000 abstract description 4
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- 230000003914 insulin secretion Effects 0.000 abstract description 4
- 230000000638 stimulation Effects 0.000 abstract description 4
- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 abstract description 4
- 229960001052 streptozocin Drugs 0.000 abstract description 4
- 238000001035 drying Methods 0.000 abstract description 2
- 150000002704 mannoses Chemical class 0.000 abstract 1
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 10
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- 239000012634 fragment Substances 0.000 description 7
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- 206010012601 diabetes mellitus Diseases 0.000 description 6
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- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 4
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- 241000894006 Bacteria Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241001523681 Dendrobium Species 0.000 description 1
- 241001678082 Dendrobium huoshanense Species 0.000 description 1
- 108010001682 Dextranase Proteins 0.000 description 1
- 208000015220 Febrile disease Diseases 0.000 description 1
- 102000051325 Glucagon Human genes 0.000 description 1
- 108060003199 Glucagon Proteins 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- PCNDJXKNXGMECE-UHFFFAOYSA-N Phenazine Natural products C1=CC=CC2=NC3=CC=CC=C3N=C21 PCNDJXKNXGMECE-UHFFFAOYSA-N 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
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- 208000031971 Yin Deficiency Diseases 0.000 description 1
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- 230000004888 barrier function Effects 0.000 description 1
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
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- MASNOZXLGMXCHN-ZLPAWPGGSA-N glucagon Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 MASNOZXLGMXCHN-ZLPAWPGGSA-N 0.000 description 1
- 229960004666 glucagon Drugs 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
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- 150000002338 glycosides Chemical class 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 239000000864 hyperglycemic agent Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 210000001542 lens epithelial cell Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
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- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 206010036067 polydipsia Diseases 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
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- 239000013589 supplement Substances 0.000 description 1
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- 208000024891 symptom Diseases 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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- Medicinal Chemistry (AREA)
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Abstract
The present invention relates to dendrobium candidum technical field, specifically related to a kind of Dendrobium officinale polysaccharide fragment and its extracting method, the extracting method includes water extraction, de- albumen, enzymolysis modification, purifying, drying and other steps, obtained polysaccharide fragment is extracted by D glucose, D mannoses, D galactolipins, L rhamnoses, D xyloses, six kinds of monose compositions of D husbands sugar, it the experiment proved that, the Dendrobium officinale polysaccharide fragment of the present invention has no stimulation to the insulin secretion of normal mouse, security is higher, and relative Glipizide antidiabetic drug, blood glucose rate of descent, insulin climbing, pancreas hyperglycaemia rate of descent is relatively good, it can be seen that the hypoglycemic effect of polysaccharide fragment of the invention SD rats diabetogenic to Streptozotocin is notable.
Description
Technical field
The present invention relates to dendrobium candidum technical field, and in particular to a kind of Dendrobium officinale polysaccharide fragment and its extracting method.
Background technology
Dendrobium candidum（Dendrobium officinale Kimura et Migo）, it is the perennial draft plant of growing nonparasitically upon another plant of orchid family
Thing.It is sweet, it is slightly cold, returns helmet, kidney channel.Successive dynasties Chinese medicine ancient books and records《Sheng Nong's herbal classic》、《A Supplement to the Compendium of Materia Medica》Deng all on the books, with
It is fresh or dry stem and be used as medicine, the effect of with nourishing Yin and clearing heat, nourishing the stomach to improve the production of body fluid, tonifying kidney and benefiting sperm.
Traditional Chinese Medicine will have drink more, eat, diuresis more, and then lean body mass or the pleasantly sweet class for cardinal symptom of urine are sick long
Disease, referred to as " quenches one's thirst ", and diabetes includes diabetes.The generation of diabetes is with kidney-yin deficiency, and scorching lung stomach is basic pathogenesis, with gas
Yin bivacuity is its pathological characteristic.Early in《The Yellow Emperor's Canon of Internal Medicine》Middle proposition therapy is based on " nourishing Yin and promoting production of body fluid, Yin Yang balancing ".《Book on Chinese herbal medicine is just》
Cloud：The stem of noble dendrobium " can anneal, yin-nourishing, relieving restlessness ... also stops hot sweat of quenching one's thirst ".《Dictionary of medicinal plant》Recording the stem of noble dendrobium, " clearing heat and nourishing yin is used for
Consumption of body fluid caused by febrile disease, dry polydipsia ".The stem of noble dendrobium can promote the production of body fluid to quench thirst, but can clearing lung-heat asthenia gastropyrexia, be exactly treat diabetes special since ancient times
Medicine.
Have that polysaccharide extract rate is low, impurity content is high, many currently for the research experiment of dendrobium candidum effect of lowering blood sugar
The shortcomings of sugared composition is indefinite, effect specificity is not strong, and extracting method is more traditional, single, obtained polysaccharide inferior quality,
There is non-special efficacy type to hypoglycemic effect.
《Dendrobidium huoshanness polysaccharide and its enzymatic fragment suppress H2O2The research of human lens epithelial cells apoptosis is induced, Lee is small
Dragon, HeFei University of Technology's master thesis》Author the polysaccharide for being named as DHPD1 is extracted from dendrobium huoshanense protocorms
Fragment, and finally shown that DHPD1 and its enzymatic fragment can significantly inhibit H2O2Induce human lens epithelial（HLE cells）Damage
The conclusion of wound and apoptosis, but it does not study the effect that the polysaccharide fragment adjusts blood glucose.The present inventor is according to described in paper
Method is extracted DHPD1 again, be found that while DHPD1 can actually alleviate Streptozotocin induction rat diabetes type it is interior in vain
Barrier, but hypoglycemic effect is not obvious.
The content of the invention
In order to overcome shortcoming and defect present in prior art, bacterium is helped to chain urea it is an object of the invention to provide a kind of
The significant Dendrobium officinale polysaccharide fragment of hypoglycemic effect of the diabetogenic SD rats of element.
Another object of the present invention is to provide a kind of extracting method of Dendrobium officinale polysaccharide fragment, the extracting method is extracted
Rate is high, impurities removing efficiency is high.
The purpose of the present invention is achieved through the following technical solutions：A kind of Dendrobium officinale polysaccharide fragment, by following Mole percent
The monose composition of ratio：
D-Glucose 11.4%-21.8%
D-MANNOSE 24.0%-36.5%
D- galactolipins 8.3%-19.0%
L- rhamnoses 13.2%-19.0%
D- xyloses 3.8%-8.6%
D- husbands sugar 7.7%-13.3%,
Wherein, the molecular weight of the Dendrobium officinale polysaccharide fragment is 3074.8-3192.6.
The polysaccharide fragment of the present invention is extracted and obtained from dendrobium candidum, the experiment proved that, Dendrobium officinale polysaccharide of the invention
Fragment has no stimulation to the insulin secretion of normal mouse, and security is higher, and relative Glipizide antidiabetic drug and
DHPD1, blood glucose rate of descent, insulin climbing, pancreas hyperglycaemia rate of descent are relatively good, it is seen that polysaccharide fragment of the invention is to chain
The hypoglycemic effect of the urea assistant diabetogenic SD rats of rhzomorph is notable.
Preferably, the Dendrobium officinale polysaccharide fragment is made up of the monose of following molar percentage：
D-Glucose 11.4%-15.2%
D-MANNOSE 26.0%-31.5%
D- galactolipins 9.4%-12.0%
The .2%-19.0% of L- rhamnoses 15
D- xyloses 3.8%-4.6%
D- husbands sugar 8.3%-9.3%.
Another goal of the invention of the present invention is achieved through the following technical solutions：A kind of extraction side of Dendrobium officinale polysaccharide fragment
Method, comprises the following steps：
（1）Water extraction：Dendrobium candidum is extracted with water, dendrobium candidum aqueous extract is obtained；
（2）De- albumen：De- albumen processing is carried out to dendrobium candidum aqueous extract using method for removing protein, crude extract is obtained；
（3）Enzymolysis modification：Addition biology enzyme agent is carried out to the crude extract to digest, and obtains mixing polysaccharide extract solution, institute
Stating biology enzyme agent includes at least one of cellulase, beta-xylanase, pectase；
（4）Purifying：Purification process is carried out to mixing polysaccharide extract solution using purification process, small molecular weight impurity is removed, obtains single
Polysaccharide extraction liquid, the small molecular weight impurity includes inorganic salts, oligosaccharide；
（5）Dry：Single polysaccharide extract solution is dried, that is, obtains described Dendrobium officinale polysaccharide fragment.
By the extracting method of the present invention, the homogeneous polysaccharide fragment of the present invention, step can be extracted from dendrobium candidum
Simply, impurities removing efficiency height, recovery rate are more than 1.2%（Recovery rate=polysaccharide fragment weight/dendrobium candidum raw material weight）.
Wherein, the step（1）Water extraction is concretely comprised the following steps：Dendrobium candidum is crushed to more than 60 mesh, ultrasonic water immersion is extracted
1-3 times, the 20-30 times of weight that water used is dendrobium candidum is extracted every time, then takes filtrate to be concentrated under reduced pressure, obtain described
Dendrobium candidum aqueous extract.Wherein ultrasonic frequency is 30-50kHz, and the time that each water logging is extracted is 10-30min.
The step of the present invention（1）The main innovation point of water extraction is to have used ultrasonic technique, many by vibrational energy cut-out
The glycosides key of glycan molecule, so that reduce polysaccharide molecular weight, promote polysaccharide water-soluble, and then it is more to improve dendrobium candidum of the present invention
The bioactivity of bglii fragment, the hypoglycemic effect response time decreases.
Wherein, the step（2）It is Deproteinated to concretely comprise the following steps：90%- is added toward described dendrobium candidum aqueous extract
95% ethanol, makes polysaccharide precipitation（The general volume fraction of ethanol made in dendrobium candidum aqueous extract is 80%-90%）, receive
Collect sediment, sediment is dissolved in the water, add Sevage reagents, centrifuged after being well mixed, take supernatant, that is, obtain described
Crude extract.
The step of the present invention（2）Deproteinated specific steps extract raw sugar sediment first with alcohol precipitation, remove water-soluble
Property and the impurity insoluble in ethanol, raw sugar sediment is redissolved in after water, yet further using Sevage reagents by albumen
Matter is precipitated out, and supernatant is obtained by centrifuging and taking.Pass through the step of the present invention（2）, by most macro-molecular protein
Remove.Preferably, the Sevage reagents are by chloroform and n-butanol by volume 5:1 ratio mixing composition, the Sevage
The volume ratio of water of the reagent with having dissolved raw sugar sediment is 1:2, and after Sevage reagents are added, rock solution 0.3-
0.7h, is then centrifuged with 900-1100r/min speed, finally obtains supernatant again.Taken by more preferably method
The supernatant obtained is relatively purer.
Wherein, the step（3）The temperature of enzymolysis modification controlled enzymatic hydrolysis is 48-50 DEG C, and the pH value of enzymolysis is 4.5-5.2,
The reaction time of enzymolysis is 70-110min, and the biology enzyme agent is dissolved in solvent by cellulase, beta-xylanase, pectase
It is made, the biology enzyme agent cellulase, beta-xylanase, the mass concentration of pectase are respectively 0.5%-0.9%, 0.3%-
0.7%、1.1%-1.5%.One of core technology point of the present invention is to combine using cellulase, beta-xylanase, pectase
Biology enzyme agent is modified polysaccharide fragment, using cellulase, beta-xylanase, pectase specificity to polysaccharide fragment
Specific glycosidic bond is degraded, and makes newly-generated non-reducing end end of travel, so as to change the monose composition of polysaccharide fragment
And paradigmatic structure, determine conspicuousness of the Dendrobium officinale polysaccharide fragment of the present invention on hypoglycemic effect.Also therefore, for fibre
The plain enzyme of dimension, beta-xylanase, the consumption selection of pectase, which have, is greatly particular about, and differently usage ratio can influence different sugar
The palliating degradation degree of glycosidic bond is different, so as to form structure, function different polysaccharide fragment completely.The present inventor passes through to enzyme
The species and consumption of enzyme are repeatedly reconfigured in solution temperature, enzymolysis pH, enzymolysis time and biology enzyme agent, then with practice
Draw a conclusion, finally drawn above-mentioned preferred scheme.
In numerous preferred schemes, it is further preferable that the biology enzyme agent cellulase, beta-xylanase, pectin
The mass concentration of enzyme is respectively 0.7%, 0.5%, 1.3%.
Wherein, the step（4）Purifying comprises the following steps：
A, supercritical extract：Extraction temperature is 40-60 DEG C, and extracting pressure is 180-220bar, and carbon dioxide flow is 15-25L/
H, extraction time is 90-150min, and single extraction product is collected every 20-40min；
B, gel filtration chromatography：Take the extraction product, upper Sephadex G-75 gel columns, with 0.5-1.5mol/L Tris-
HCl buffer solutions are eluted with 1-3ml/min flow velocity, are detected with sulfuric acid-phynol method, obtain refined solution；
C, dialysis：Refined solution is dialysed 10-14h at a temperature of 0-8 DEG C, the single polysaccharide extract solution is obtained.
Pass through above-mentioned steps（4）What decimal was difficult to remove in the concrete operations of purifying, polysaccharide fragment of further having gone out is small
Molecular impurity.Although the present invention supercritical extract, gel filtration chromatography and dialyse it is single for, be the weight for prior art
New settings, but for different molecular weight, the polysaccharide fragment of structure, prior art often can only be general, but extraction efficiency may
Sorrow, in some instances it may even be possible to the larger other polysaccharide fragments of molecular weight difference are mixed into the polysaccharide fragment that the present invention can be made, so as to influence many
The homogeneity of bglii fragment.The present inventor is used by being combined to supercritical extract, gel filtration chromatography and dialysis, and to parameter
Specific aim setting is carried out, so that for the relatively conventional method of purification process of the present invention and setting, recovery rate is improved
More than 10%.
In numerous preferred purification process, recovery rate relatively conventional method and setting improve 15% and are more highly preferred to
Scheme be：The step（4）Purifying comprises the following steps：
A, supercritical extract：Extraction temperature is 50 DEG C, and extracting pressure is 200bar, and carbon dioxide flow is 20L/h, extraction time
For 120min, single extraction product is collected every 30min；
B, gel filtration chromatography：The extraction product is taken, upper Sephadex G-75 gel columns are buffered with 1mol/L Tris-HCl
Liquid is eluted with 2ml/min flow velocity, is detected with sulfuric acid-phynol method, obtains refined solution；
C, dialysis：Refined solution is dialysed 12h at a temperature of 4 DEG C, the single polysaccharide extract solution is obtained.
Wherein, the step（5）Dry concretely comprises the following steps：Single polysaccharide extract solution is subjected to decompression freeze-drying, i.e.,
Obtain described Dendrobium officinale polysaccharide fragment.Destructiveness of other the relative heated drying modes of decompression freeze-drying for polysaccharide fragment
It is lower, as specific temperature, air pressure and time can be -50~-30 DEG C, 5-15Pa, 3-5h, can also be according to prior art
Set, be not repeated herein.
The beneficial effects of the present invention are：1st, polysaccharide fragment of the invention is extracted and obtained from dendrobium candidum, is demonstrate,proved through experiment
Bright, Dendrobium officinale polysaccharide fragment of the invention has no stimulation to the insulin secretion of normal mouse, and security is higher, Er Qiexiang
To Glipizide antidiabetic drug and DHPD1, blood glucose rate of descent, insulin climbing, pancreas hyperglycaemia rate of descent are relatively good, it is seen that
The hypoglycemic effect of the polysaccharide fragment SD rats diabetogenic to Streptozotocin of the present invention is notable；2nd, extraction side of the invention
Method step is simple, and recovery rate and impurities removing efficiency are high, the polysaccharide fragment Stability Analysis of Structures extracted, it is easy to promote.
Embodiment
For the ease of the understanding of those skilled in the art, with reference to embodiment, the present invention is further illustrated, real
The content that the mode of applying is referred to not limitation of the invention.
Embodiment 1
The present embodiment extracts described Dendrobium officinale polysaccharide fragment as follows：
（1）Water extraction：Dendrobium candidum is crushed to more than 60 mesh, ultrasonic water immersion is extracted 3 times, it is iron sheet stone that water used is extracted every time
25 times of weight of dry measure used in former times, then take filtrate to be concentrated under reduced pressure, and obtain described dendrobium candidum aqueous extract；Wherein ultrasonic frequency
For 40kHz, the time that each water logging is extracted is 20min.
（2）De- albumen：Toward the ethanol of described dendrobium candidum aqueous extract addition 95%, make in dendrobium candidum aqueous extract
Volume fraction of ethanol be 90%, collect sediment, sediment is dissolved in the water, add Sevage reagents, rock solution
0.5h, is then centrifuged with 1000r/min speed again, finally takes supernatant, that is, obtains the crude extract, wherein, institute
Sevage reagents are stated by chloroform and n-butanol by volume 5:1 ratio mixing composition, the Sevage reagents are with having dissolved institute
The volume ratio for stating the water of sediment is 1:2；
（3）Enzymolysis modification：Addition biology enzyme agent is carried out to the crude extract to digest, and obtains mixing polysaccharide extract solution, its
In, the temperature of enzymolysis modification controlled enzymatic hydrolysis is 49 DEG C, and the pH value of enzymolysis is 4.8, and the reaction time of enzymolysis is 90min, the life
The agent of thing enzyme is dissolved in solvent by cellulase, beta-xylanase, pectase to be made, the biology enzyme agent cellulase, β-wood
Dextranase, the mass concentration of pectase are respectively 0.7%, 0.5%, 1.3%.
（4）Purifying：Comprise the following steps：A, supercritical extract：Extraction temperature is 50 DEG C, and extracting pressure is 200bar, two
Oxidation carbon flow is 20L/h, and extraction time is 120min, and single extraction product is collected every 30min；B, gel filtration chromatography：Take
The extraction product, upper Sephadex G-75 gel columns, with 1.0mol/L Tris-HCl buffer solutions with 2ml/min flow velocity
Eluted, detected with sulfuric acid-phynol method, obtain refined solution；C, dialysis：Refined solution is dialysed 12h at a temperature of 4 DEG C, obtained
The single polysaccharide extract solution.
（5）Dry：Single polysaccharide extract solution is subjected to decompression freeze-drying, that is, obtains described Dendrobium officinale polysaccharide piece
Section.
Embodiment 2
The present embodiment and the difference of embodiment 1 are：
The step（1）In water extraction, ultrasonic water immersion extraction time is 1 time, extracts 20 times of weight that water used is dendrobium candidum；
The frequency of ultrasound is 30kHz, and the time that each water logging is extracted is 30min.
The step（2）It is 90% toward the ethanol volumetric concentration that described dendrobium candidum aqueous extract is added in de- albumen,
It is 80% to make volume fraction of ethanol in dendrobium candidum aqueous extract, and solution 0.3h is rocked after adding Sevage reagents, then again with
900r/min speed is centrifuged, and finally takes supernatant；
The step（3）In enzymolysis modification, the temperature of enzymolysis modification controlled enzymatic hydrolysis is 48 DEG C, and the pH value of enzymolysis is 4.5, enzymolysis
Reaction time is 70min, and the biology enzyme agent is dissolved in solvent by cellulase, beta-xylanase, pectase to be made, the life
Thing enzyme agent cellulase, beta-xylanase, the mass concentration of pectase are respectively 0.5%, 0.3%, 1.1%；
The step（4）In purifying, the extraction temperature of step A is 40 DEG C, and extracting pressure is 180bar, and carbon dioxide flow is
15L/h, extraction time is 90min, and the time interval for collecting extraction product is 20min；The Tris-HCl buffer concentrations of step B
For 0.5mol/L, flow velocity is 1ml/min；Step C dialysis temperature is 0 DEG C, and the time is 10h.
Embodiment 3
The present embodiment and the difference of embodiment 1 are：
The step（1）In water extraction, ultrasonic water immersion extraction time is 2 times, extracts 20 times of weight that water used is dendrobium candidum；
The frequency of ultrasound is 50kHz, and the time that each water logging is extracted is 10min.
The step（2）It is 92% toward the ethanol volumetric concentration that described dendrobium candidum aqueous extract is added in de- albumen,
It is 82% to make volume fraction of ethanol in dendrobium candidum aqueous extract, and solution 0.7h is rocked after adding Sevage reagents, then again with
1100r/min speed is centrifuged, and finally takes supernatant；
The step（3）In enzymolysis modification, the temperature of enzymolysis modification controlled enzymatic hydrolysis is 50 DEG C, and the pH value of enzymolysis is 5.2, enzymolysis
Reaction time is 110min, and the biology enzyme agent is dissolved in solvent by cellulase, beta-xylanase, pectase to be made, described
Biology enzyme agent cellulase, beta-xylanase, the mass concentration of pectase are respectively 0.9%, 0.7%, 1.5%；
The step（4）In purifying, the extraction temperature of step A is 60 DEG C, and extracting pressure is 220bar, and carbon dioxide flow is
25L/h, extraction time is 150min, and the time interval for collecting extraction product is 40min；The Tris-HCl buffer solutions of step B are dense
Spend for 1.5mol/L, flow velocity is 3ml/min；Step C dialysis temperature is 8 DEG C, and the time is 14h.
Embodiment 4
The present embodiment and the difference of embodiment 1 are：
The step（1）In water extraction, ultrasonic water immersion extraction time is 3 times, extracts 28 times of weight that water used is dendrobium candidum；
The step（2）In de- albumen, the ethanol volumetric concentration added toward described dendrobium candidum aqueous extract is 95%, makes iron
Volume fraction of ethanol in skin stem of noble dendrobium aqueous extract is 85%, and solution 0.4h is rocked after adding Sevage reagents, then again with
950r/min speed is centrifuged, and finally takes supernatant；
The step（3）In enzymolysis modification, the temperature of enzymolysis modification controlled enzymatic hydrolysis is 49 DEG C, and the pH value of enzymolysis is 5.1, enzymolysis
Reaction time is 100min, and the biology enzyme agent is dissolved in solvent by cellulase, beta-xylanase, pectase to be made, described
Biology enzyme agent cellulase, beta-xylanase, the mass concentration of pectase are respectively 0.8%, 0.4%, 1.2%；
The step（4）In purifying, the extraction temperature of step A is 55 DEG C, and extracting pressure is 190bar, and carbon dioxide flow is
22L/h, extraction time is 100min, and the time interval for collecting extraction product is 30min；The Tris-HCl buffer solutions of step B are dense
Spend for 1.2mol/L, flow velocity is 2.3ml/min；Step C dialysis temperature is 2 DEG C, and the time is 11h.
Embodiment 5
The present embodiment and the difference of embodiment 1 are：
The step（1）In water extraction, ultrasonic water immersion extraction time is 2 times, extracts 27 times of weight that water used is dendrobium candidum；
The step（2）In de- albumen, the ethanol volumetric concentration added toward described dendrobium candidum aqueous extract is 93%, makes iron
Volume fraction of ethanol in skin stem of noble dendrobium aqueous extract is 84%, and solution 0.6h is rocked after adding Sevage reagents, then again with
980r/min speed is centrifuged, and finally takes supernatant；
The step（3）In enzymolysis modification, the temperature of enzymolysis modification controlled enzymatic hydrolysis is 49 DEG C, and the pH value of enzymolysis is 4.6, enzymolysis
Reaction time is 80min, and the biology enzyme agent is dissolved in solvent by cellulase, beta-xylanase, pectase to be made, the life
Thing enzyme agent cellulase, beta-xylanase, the mass concentration of pectase are respectively 0.8%, 0.4%, 1.4%；
The step（4）In purifying, the extraction temperature of step A is 55 DEG C, and extracting pressure is 195bar, and carbon dioxide flow is
23L/h, extraction time is 135min, and the time interval for collecting extraction product is 25min；The Tris-HCl buffer solutions of step B are dense
Spend for 1.1mol/L, flow velocity is 2.6ml/min；Step C dialysis temperature is 6 DEG C, and the time is 12h.
Embodiment 6
The present embodiment and the difference of embodiment 1 are：
The step（3）In enzymolysis modification, the biology enzyme agent cellulase, beta-xylanase, the mass concentration of pectase
Respectively 1.2%, 1.0%, 1.0%.
It is measured by molecular weight and monose composition that obtained Dendrobium officinale polysaccharide fragment is extracted to embodiment 1-6,
It is 3074.8-3192.6 that embodiment 1-6, which extracts obtained polysaccharide fragment molecular weight, by D-Glucose, D-MANNOSE, D- galas
Sugar, L- rhamnoses, D- xyloses, D- husbands sugar six kinds of monose composition, the molar percentage of every kind of monose be followed successively by 11.4%-21.8%,
24.0%-36.5%、8.3%-19.0%、13.2%-19.0%、3.8%-8.6%、7.7%-13.3%。
Embodiment 1 extracts obtained Dendrobium officinale polysaccharide fragment blood sugar decreasing effect test：
1st, influence of the Dendrobium officinale polysaccharide fragment to normal SD rat blood sugar, serum insulin.
Test method is：Normal rat 36 is taken, is divided into 6 groups, the mL/kg of physiological saline 20, positive drug are given respectively（Lattice are arranged
Pyrazine antidiabetic drug）0.0025 g/kg, DHPD1 polysaccharide fragment 0.4 g/kg, the g/kg of dendrobium candidum Thick many candies 0.4, dendrobium candidum
The g/kg of polysaccharide fragment 0.2,0.4 g/kg, 0.8 g/kg, are administered once a day, for three days on end, are taken in 2 h dockings after last dose
Blood, blood glucose is surveyed with glucose oxidase method, is surveyed insulin with radioimmunology, is obtained table 1 below：
Influence of the Dendrobium officinale polysaccharide fragment of table 1 to normal SD rat blood sugar, serum insulin
* with * *：P when being compared with blank control group<0.05, P<0.01.
As can be seen from the above table, be administered 3 days after, compared with physiological saline group, Dendrobium officinale polysaccharide fragment it is basic, normal, high
Dosage is statistically without significant difference, and dendrobium candidum Thick many candies have a certain impact to blood glucose value and serum insulin, say
The Dendrobium officinale polysaccharide fragment that the bright present invention is provided, without influence, illustrates the iron sheet that the present invention is provided to the fasting blood-glucose of normal rat
Dendrobium polysaccharide fragment has no stimulation to the insulin secretion of normal mouse, compared to dendrobium candidum Thick many candies, what the present invention was provided
Dendrobium officinale polysaccharide fragment security is higher.
2nd, Dendrobium officinale polysaccharide fragment to Streptozotocin-SD rat blood sugars, serum insulin, hyperglycemic factor shadow
Ring.
Test method is：（1）Modeling：The h of SD Rat Fasts 16, weighs, after injection Streptozotocin 70 mg/kg, 72 h
Docking takes blood, then determines fasting blood sugar, takes blood glucose value to be for experiment more than 16 mmol/L；（2）Diabetes rat 48 is taken,
6 groups are randomly divided into, the mL/kg of physiological saline 20, positive control drug are given respectively（Glipizide antidiabetic drug）0.0025 g/kg, iron sheet
The g/kg of stem of noble dendrobium Thick many candies 0.4, the g/kg of Dendrobium officinale polysaccharide fragment 0.2,0.4 g/kg, 0.8 g/kg, is administered once a day, even
Continuous 9 days, determine respectively initial blood glucose value and the 3rd day, the 6th day, the fasting blood sugar of the 9th day, serum insulin, pancreas hyperglycaemia
Element, obtains table 2-4.
Every testing result after the medication of table 2 the 3rd day
* with * *：Compared P with blank control group<0.05, P<0.01.
Every testing result after the medication of table 3 the 6th day
* with * *：Compared P with blank control group<0.05, P<0.01.
Every testing result after the medication of table 4 the 9th day
* with * *：Compared P with blank control group<0.05, P<0.01.
Shown by table 2-4, the Dendrobium officinale polysaccharide fragment that the present invention is provided can significantly reduce Streptozotocin-SD rats
Blood sugar level, improves serum insulin content, and the release of glucagon suppression, compared with blank control, there were significant differences,
With positive controls（Glipizide antidiabetic drug）Compare, when medication was by the 9th day, polysaccharide fragment group high dose（0.8 g/kg/d）
Blood glucose rate of descent, insulin climbing, pancreas hyperglycaemia rate of descent it is higher than positive controls.In addition, compared with Thick many candies, it is many
The blood sugar decreasing effect of bglii fragment is more obvious, and increases with administration time, and action effect increases therewith.
Above-described embodiment is the present invention preferably implementation, and in addition, the present invention can be realized with other manner,
Any obvious replacement is within protection scope of the present invention on the premise of not departing from present inventive concept.
Claims (10)
1. a kind of Dendrobium officinale polysaccharide fragment, it is characterised in that：It is made up of the monose of following molar percentage：
D-Glucose 11.4%-21.8%
D-MANNOSE 24.0%-36.5%
D- galactolipins 8.3%-19.0%
L- rhamnoses 13.2%-19.0%
D- xyloses 3.8%-8.6%
D- husbands sugar 7.7%-13.3%,
Wherein, the molecular weight of the Dendrobium officinale polysaccharide fragment is 3074.8-3192.6.
2. a kind of Dendrobium officinale polysaccharide fragment according to claim 1, it is characterised in that：By the list of following molar percentage
Sugar composition：
D-Glucose 13.4%-17.9%
D-MANNOSE 29.0%-33.5%
D- galactolipins 10.4%-14.9%
The .2%-19.0% of L- rhamnoses 15
D- xyloses 3.8%-7.6%
D- husbands sugar 8.3%-12.3%.
3. a kind of extracting method of Dendrobium officinale polysaccharide fragment as claimed in claim 1 or 2, it is characterised in that：Including as follows
Step：
（1）Water extraction：Dendrobium candidum is extracted with water, dendrobium candidum aqueous extract is obtained；
（2）De- albumen：De- albumen processing is carried out to dendrobium candidum aqueous extract, crude extract is obtained；
（3）Enzymolysis modification：Biology enzyme agent is added to the crude extract to digest, and obtains mixing polysaccharide extract solution, the life
The agent of thing enzyme includes at least one of cellulase, beta-xylanase, pectase；
（4）Purifying：Purification process is carried out to mixing polysaccharide extract solution, small molecular weight impurity is removed, obtains single polysaccharide extract solution；
（5）Dry：Single polysaccharide extract solution is dried, that is, obtains described Dendrobium officinale polysaccharide fragment.
4. a kind of extracting method of Dendrobium officinale polysaccharide fragment according to claim 3, it is characterised in that：The step
（1）In, water extraction is concretely comprised the following steps：Dendrobium candidum is crushed to more than 60 mesh, ultrasonic water immersion is extracted 1-3 times, and institute is extracted every time
Water is 20-30 times of weight of dendrobium candidum, then takes filtrate to be concentrated under reduced pressure, and obtains described dendrobium candidum water and extracts
Liquid.
5. a kind of extracting method of Dendrobium officinale polysaccharide fragment according to claim 3, it is characterised in that：The step
（2）In, it is Deproteinated to concretely comprise the following steps：The ethanol that mass fraction is 90%-95% is added toward described dendrobium candidum aqueous extract,
Make polysaccharide precipitation, collect sediment, sediment is dissolved in the water, add Sevage reagents, centrifuged after being well mixed, take supernatant
Liquid, that is, obtain the crude extract.
6. a kind of extracting method of Dendrobium officinale polysaccharide fragment according to claim 3, it is characterised in that：The step
（3）In, the temperature of enzymolysis is 48-50 DEG C, and the pH value of enzymolysis is 4.5-5.2, and the reaction time of enzymolysis is 70-110min, described
Biology enzyme agent is dissolved in solvent by cellulase, beta-xylanase, pectase to be made, and the biology enzyme agent cellulase, β-
Zytase, the mass fraction of pectase are respectively 0.5%-0.9%, 0.3%-0.7%, 1.1%-1.5%.
7. a kind of extracting method of Dendrobium officinale polysaccharide fragment according to claim 6, it is characterised in that：The biology enzyme
Agent cellulase, beta-xylanase, the mass fraction of pectase are respectively 0.7%, 0.5%, 1.3%.
8. a kind of extracting method of Dendrobium officinale polysaccharide fragment according to claim 3, it is characterised in that：The step
（4）In, purifying comprises the following steps：
A, supercritical extract：Extraction temperature is 40-60 DEG C, and extracting pressure is 180-220bar, and carbon dioxide flow is 15-25L/
H, extraction time is 90-150min, and single extraction product is collected every 20-40min；
B, gel filtration chromatography：Take the extraction product, upper Sephadex G-75 gel columns, with 0.5-1.5mol/L Tris-
HCl buffer solutions are eluted with 1-3mL/min flow velocity, are detected with sulfuric acid-phynol method, obtain refined solution；
C, dialysis：Refined solution is dialysed 10-14h at a temperature of 0-8 DEG C, the single polysaccharide extract solution is obtained.
9. a kind of extracting method of Dendrobium officinale polysaccharide fragment according to claim 8, it is characterised in that：The step
（4）Purifying comprises the following steps：
A, supercritical extract：Extraction temperature is 50 DEG C, and extracting pressure is 200bar, and carbon dioxide flow is 20L/h, extraction time
For 120min, single extraction product is collected every 30min；
B, gel filtration chromatography：The extraction product is taken, upper Sephadex G-75 gel columns are buffered with 1mol/L Tris-HCl
Liquid is eluted with 2mL/min flow velocity, is detected with sulfuric acid-phynol method, obtains refined solution；
C, dialysis：Refined solution is dialysed 12h at a temperature of 4 DEG C, the single polysaccharide extract solution is obtained.
10. a kind of extracting method of Dendrobium officinale polysaccharide fragment according to claim 3, it is characterised in that：The step
（5）In, that dries concretely comprises the following steps：Single polysaccharide extract solution is subjected to decompression freeze-drying, that is, obtains described dendrobium candidum
Polysaccharide fragment.
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Cited By (5)
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| CN110343191A (en) * | 2019-08-27 | 2019-10-18 | 平顶山学院 | The extraction of Funiu Shan Mountain Dendrobium nobile polysaccharide and the method for preparing solid beverage |
| WO2022183763A1 (en) * | 2021-03-05 | 2022-09-09 | 中国科学院昆明植物研究所 | Dendrobium officinale oligosaccharide and derivative thereof, and preparation methods therefor and applications thereof |
| CN117106764A (en) * | 2023-08-28 | 2023-11-24 | 东莞巨微新材料科技有限公司 | Immobilized complex enzyme for preparing ultralow molecular dendrobium candidum polysaccharide and preparation method and application thereof |
| CN117965486A (en) * | 2024-02-08 | 2024-05-03 | 皖西学院 | Dendrobium huoshanense UDP-rhamnose synthetase and application thereof |
| CN117965486B (en) * | 2024-02-08 | 2024-07-16 | 皖西学院 | Dendrobium huoshanense UDP-rhamnose synthetase and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110343191A (en) * | 2019-08-27 | 2019-10-18 | 平顶山学院 | The extraction of Funiu Shan Mountain Dendrobium nobile polysaccharide and the method for preparing solid beverage |
| WO2022183763A1 (en) * | 2021-03-05 | 2022-09-09 | 中国科学院昆明植物研究所 | Dendrobium officinale oligosaccharide and derivative thereof, and preparation methods therefor and applications thereof |
| CN117106764A (en) * | 2023-08-28 | 2023-11-24 | 东莞巨微新材料科技有限公司 | Immobilized complex enzyme for preparing ultralow molecular dendrobium candidum polysaccharide and preparation method and application thereof |
| CN117106764B (en) * | 2023-08-28 | 2024-02-23 | 东莞巨微新材料科技有限公司 | Immobilized complex enzyme for preparing ultralow molecular dendrobium candidum polysaccharide and preparation method and application thereof |
| CN117965486A (en) * | 2024-02-08 | 2024-05-03 | 皖西学院 | Dendrobium huoshanense UDP-rhamnose synthetase and application thereof |
| CN117965486B (en) * | 2024-02-08 | 2024-07-16 | 皖西学院 | Dendrobium huoshanense UDP-rhamnose synthetase and application thereof |
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