CN106244390A - A kind of Herba Dendrobii Cordyceps fermentation original plasm wine and preparation method thereof - Google Patents
A kind of Herba Dendrobii Cordyceps fermentation original plasm wine and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to the preparation method of a kind of Herba Dendrobii Cordyceps fermentation original plasm wine, comprise the steps: to pull an oar to obtain stem of Dendrobium bar Herba Dendrobii juice；Use cellulase and pectase associating enzymolysis processing, obtain enzymolysis Herba Dendrobii juice；In described enzymolysis Herba Dendrobii juice, add carbon source and nitrogen source, after sterilizing, obtain Herba Dendrobii juice Cordyceps fermentation culture；Cordyceps seed liquor subcultivation is entered described Herba Dendrobii juice Cordyceps fermentation culture, Herba Dendrobii juice Cordyceps fermentation oleo stock of fermenting to obtain；After described Herba Dendrobii juice Cordyceps fermentation oleo stock homogenizing, nano-level grinder is used to process；Oleo stock after processing mixes with wine, after aging and get final product.The method cellulase degradation Herba Dendrobii juice that the present invention provides, favourable Herba Dendrobii alkaloid and dendrobium polysaccharide discharge from Herba Dendrobii, dissolution；With Cordyceps fermentation Herba Dendrobii juice, control dendrobium polysaccharide degraded, be greatly improved Herba Dendrobii utilization rate；Gained original plasm wine contains the pharmaceutical chemistry composition of Herba Dendrobii and Cordyceps, has Herba Dendrobii and Cordyceps is medicinal and health care.
Description
Technical field
The present invention relates to the processing of wine product and application, be specifically related to a kind of Herba Dendrobii juice Cordyceps fermentation original plasm wine and system thereof
Preparation Method.
Background technology
Cordyceps is traditional famous and precious of China, rare Chinese medicine, and medication is with a long history, has cough-relieving, reduces phlegm, and reduces blood
Fat, improves the pharmacological actions such as menoxenia, sexual impotence, antitumor, enhancing immunity.From fresh Cordyceps, separation obtains medicinal
Fungus, produces with artificial fermentation technique and obtains Cordyceps mycelium, has and makees with natural cordyceps ingredient and pharmacology
With, it is widely applied clinically.
Herba Dendrobii is the orchid family, Dendrobium, herbaceos perennial, and Herba Dendrobii and Ganoderma, Cordyceps, Radix Ginseng, Herba Cistanches are with claiming
For the big Herba mesonae chinensis of China's medical science five, Herba Dendrobii among the people is called ＂ help Herba mesonae chinensis ＂, medicine gold, and Compendium of Materia Medica describes；Herba Dendrobii reinforcing YIN-essence benefit essence is thick
Road, flat gastric qi, Fructus Alpiniae Oxyphyllae, hepatoprotective function of gallbladder promoting.Modern Chinese medicine research shows: containing a large amount of polysaccharide and multiple alkaloid in stem of Dendrobium
Deng pharmaceutical composition, there is nourishing YIN and moistening the lung, stomach reinforcing the kidney invigorating, tonifying five ZANG-organs asthenia, clearing away lung-heat to relieve cough, brain-strengthening, improving eyesight, resisting fatigue, carry
High body immunity, defying age, suppresses the effects such as tumor, expansion blood vessel.
In recent years, in some provinces and cities of China, as food and medicament dual-purpose plant, oneself obtains terrestrial reference registration, the health-care effect of Herba Dendrobii to Herba Dendrobii
Also obtain the accreditation of more and more people with medical value, make product with Herba Dendrobii for raw material and be no longer confined to Herba Dendrobii or Herba Dendrobii wind
Bucket, other Herba Dendrobii goods with certain health care or medicinal function are also many, and dendrobium wine is the one of Herba Dendrobii research and development in recent years
Plant one of staple product.
Present stage, the method making dendrobium wine is substantially continued to use traditional medicated wine brewing method, is limited to use soaking in Chinese liquor stone
Dry measure used in former times and other Chinese medicines, in medical material, active component dissolution is limited, affects product volume and using effect；Even if soaking Chinese medicine process
And add reflux, extract, improve the dissolution of medical material soluble components to a certain extent, but ignore many Herba Dendrobii active component
In Chinese liquor (ethanol water) solution, dissolubility is low, and equally, Herba Dendrobii and other Chinese medicine can not be fully used.
Although, prior art makes the method for dendrobium wine in addition to tradition immersion process, the making of the most useful fermentation method
Method.If but only with before soaking in Chinese liquor Herba Dendrobii operation, adding and make Chinese liquor operation with Herba Dendrobii, Semen Maydis etc., ignore stone
The active component of dry measure used in former times is high boiling substance mostly, and the operation increasing Herba Dendrobii making Chinese liquor the most seldom improves effective utilization of Herba Dendrobii；
Or add glucoamylase at saccharifying, and in the case of lacking enzymolysis control condition, may be the dendrobium polysaccharide in big portion
It is degraded into monosaccharide, reduces the health-care effect of dendrobium polysaccharide.
Summary of the invention
For overcoming the defect of above-mentioned prior art, the present invention proposes the preparation side of a kind of Herba Dendrobii juice Cordyceps fermentation original plasm wine
Method, the method is first by the dissolution from Herba Dendrobii to greatest extent of Herba Dendrobii effective ingredient, the fermentation technology pair of recycling Cordyceps fungus
Herba Dendrobii active ingredient carries out controllability fermentation, prepares one more conducively Herba Dendrobii and efficiently utilizes, has complete Herba Dendrobii original pharmacology merit
The Herba Dendrobii juice Cordyceps fermentation original plasm wine of Cordyceps pharmacological action can be had again concurrently.
Specifically, the preparation method of the Herba Dendrobii juice Cordyceps fermentation original plasm wine that the present invention provides, comprise the following steps:
(1) stem of Dendrobium bar is pulled an oar to obtain Herba Dendrobii juice；
(2) use cellulase and pectase associating enzymolysis processing, obtain enzymolysis Herba Dendrobii juice；
(3) in described enzymolysis Herba Dendrobii juice, add carbon source and nitrogen source, after sterilizing, obtain Herba Dendrobii juice Cordyceps fermentation culture；
(4) Cordyceps seed liquor subcultivation is entered described Herba Dendrobii juice Cordyceps fermentation culture, Herba Dendrobii juice Cordyceps of fermenting to obtain
Fermentation oleo stock；
(5) by after described Herba Dendrobii juice Cordyceps fermentation oleo stock homogenizing, nano-level grinder is used to process；
(6) will mix with wine through the oleo stock after step (5) processes, aging, obtain Herba Dendrobii juice Cordyceps fermentation original plasm wine.
In described step (1), Herba Dendrobii is preferably purple dendrobium or Herba Dendrobii.In this step, stem of Dendrobium bar is removed the peel, clearly
Xian is clean, is cut into 0.3～1cm segment, is 2～6% addition pure water by the weight ratio of Herba Dendrobii Yu water, preferably water temperature 90～95 degree
Pure water, at a temperature of drop to temperature 35～when 55 degree, pull an oar 3～6 minutes with 800～1500rpm high speed beaters, obtain Herba Dendrobii
Juice.Preferably, Herba Dendrobii is 3～4% with the w/v of purified water.Preferably, the temperature of water temperature is 90 degree, and soak time is
8～10 minutes.
In described step (2), it is to use cellulase and pectase associating enzymolysis processing Herba Dendrobii that described complex enzyme hydrolysis processes
Juice.In order to realize good hydrolysis result, adding the work of total enzyme in Herba Dendrobii juice described in every 100ml is 1 × 104U～25 × 104The fibre of u
Dimension element enzyme and pectase carry out associating enzymolysis processing；The amount ratio of the most described cellulase and pectase is 1:0.5～3.Make
For a kind of specific embodiments of the present invention, the liquid edible level cellulase preparation that enzyme activity can be used to be 100,000 u/ml and enzyme
Vigor is the liquid pectin enzyme preparation of 50,000 u/ml, and the volume adding enzyme accounts for the 0.2～2.5% of described Herba Dendrobii juice volume, is preferably
0.5～1%.During described associating enzymolysis processing, hydrolysis temperature is 40～60 degree, and enzymolysis time is 0.5～2.5 hour；Excellent
Selection of land, hydrolysis temperature is 55 degree, and enzymolysis time is 0.5～1 hour.
In described step (3), in enzymolysis Herba Dendrobii juice add carbon source be 0.8～1.6g/100ml glucose, 0.5～
1.0g/100ml sucrose, adding nitrogen source in enzymolysis Herba Dendrobii juice is 0.6～3.0g/100ml soybean cake powder, 0.5～1.0g/100ml
Peptone, and sterilization treatment 20～25 minutes under 115～123 degree, obtain Herba Dendrobii juice Cordyceps fermentation medium.Preferably,
Glucose addition is 1.2g/100ml, and sucrose addition is 0.6g/100ml, and soybean cake powder addition is 1.6g/100ml.Preferably
Ground, the most suitable temperature of Herba Dendrobii juice sterilization treatment is 115 degree, and the process time is 20 minutes.
In described step (4), the most cultured Cordyceps fungus liquid seeds is sent out by accounting for sent Herba Dendrobii juice Cordyceps
Ferment culture volume 6～the ratio of 15%, subcultivation enters Herba Dendrobii juice Cordyceps fermentation medium, and is 19～25 degree at fermentation temperature
Lower air agitation is fermented.After fermentation time reaches 36 hours, often it is separated by 2 hours with iodine liquid off-line and picks up and look into the fermentation of Herba Dendrobii juice Cordyceps
Oleo stock, controls the degree of degradation of dendrobium polysaccharide, when fermentation oleo stock Iod R disappears, terminates course of fermentation.Preferably, subcultivation amount
The suitableeest 8%, the suitableeest control temperature is 23 degree.
Wherein, liquid seeds cultivate be from fresh natural cordyceps separate, purification, and through molecular biological characteristics reflect
It is set to Cordyceps, inoculates into following culture medium by 0.5g/100ml: Rhizoma Solani tuber osi 20g/100ml, glucose 1.2g/100ml, albumen
Peptone 0.6g/100ml, yeast extract 0.15g/100ml, surplus is water, 19～25 degree of ventilation shaken cultivation 42～62 hours.Preferably
Ground, described Cordyceps is isolated and purified from Nagqu natural cordyceps,
In described step (5), Herba Dendrobii juice Cordyceps fermentation oleo stock inputs high-pressure fluid nanometer mill after homogenizer processes, and receives
Rice mill operation temperature is 75～90 degree, and operation pressure is 80～120MPa.Preferably, nanometer mill operation temperature is 76～87 degree,
Pressure is 100MPa.
In described step (6), the alcoholic strength adding pure grain rice wine is 56～72 degree, Herba Dendrobii Cordyceps fermentation oleo stock and rice wine
Ratio is 1:1～1.5, and aging temperature is 20～35 degree, and the aging time is 15～30 days, the Herba Dendrobii Cordyceps original plasm wine made.
As the preferred version of the present invention, described method includes step in detail below:
(1) take Herba Dendrobii stem bar, remove crust, clean up, be cut into 0.3～0.6cm segment, by the weight of Herba Dendrobii Yu water
Amount ratio is 2～5:100 90～95 DEG C of water of addition, after water temperature drop to 40～45 DEG C, and making beating 3 under rotating speed 1000～1500rpm
～5 minutes, filter crude fibre, obtain Herba Dendrobii juice；
(2), in Herba Dendrobii juice described in every 100ml, adding the work of total enzyme is 1 × 104U～25 × 104The cellulase of u and pectase
Carrying out associating enzymolysis processing, the amount ratio of described cellulase and pectase is 1:0.5～3；At 55 DEG C enzymolysis 40～
60min, filters dendrobium fiber, obtains enzymolysis Herba Dendrobii juice；
(3) in described enzymolysis Herba Dendrobii juice, glucose 1.2g/100ml, sucrose 0.8g/100ml, soybean cake powder juice are added
1.0g/100ml and peptone 0.5g/100ml；Sterilization treatment 20min at 110～115 DEG C, obtains the fermentation of Herba Dendrobii juice Cordyceps
Culture fluid；
(4) from fresh natural cordyceps separation, the Cordyceps strain of purification, access by the amount of 0.5g/100ml
The Cordyceps liquid seed culture medium of following composition: Rhizoma Solani tuber osi 20g/100ml, glucose 1.2g/100ml, peptone 0.6g/
100ml, yeast extract 0.15 g/100ml, surplus is water；Cultivate 46 hours at temperature 22 DEG C, obtain Cordyceps seed liquor；
Described Cordyceps seed liquor is entered in described Herba Dendrobii juice Cordyceps fermentation culture with 8～15ml/100ml subcultivation,
In 21～23 DEG C, air agitation fermentation under the conditions of liquid wind volume ratio 1:0.4～0.7, from sweat 36～44 hours, every phase
Checked with iodine liquid off-line every 2 hours, to control the palliating degradation degree of dendrobium polysaccharide, when iodine liquid loss for reaction, terminate fermentation, obtain stone
Dry measure used in former times juice Cordyceps fermentation oleo stock；
(5) by described Herba Dendrobii juice Cordyceps fermentation oleo stock input homogenizer, 45 DEG C, carry out homogenizing under the conditions of 90MPa, will
Homogenizer effluent sends into high-pressure fluid nanometer mill, controls nanometer mill operation temperature 78～85 DEG C, operation pressure be 80～
120MPa；
(6) by pure grain rice wine that the Herba Dendrobii Cordyceps processed through step (5) fermentation oleo stock and alcoholic strength are 56～72 degree by
Volume ratio 1:1～1.2 mixing, aging 20～25 days at 25 DEG C, obtain Herba Dendrobii Cordyceps fermentation original plasm wine.
The present invention protects the Herba Dendrobii Cordyceps fermentation original plasm wine that described method is prepared from simultaneously.The Herba Dendrobii that the present invention provides
In Cordyceps fermentation original plasm wine, dendrobium total alkaloids, polysaccharide be (Herba Dendrobii Cordyceps, Cordyceps adenosine, cordycepin content and cordycepic acid
Content is the highest.
Herba Dendrobii is prepared as Herba Dendrobii juice cellulose-binding enzyme enzymolysis Herba Dendrobii juice by the scheme that the present invention provides, and favourable Herba Dendrobii is raw
Alkaloids and dendrobium polysaccharide discharge from Herba Dendrobii, dissolution；With Cordyceps fermentation Herba Dendrobii juice, control dendrobium polysaccharide degraded, be greatly improved
Herba Dendrobii utilization rate；The Herba Dendrobii Cordyceps fermentation original plasm wine prepared with Cordyceps fermentation Herba Dendrobii juice, contains Herba Dendrobii and worm summer in winter simultaneously
The pharmaceutical chemistry composition of grass, has Herba Dendrobii and Cordyceps is medicinal and health care.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.
Embodiment 1
Present embodiments provide a kind of Herba Dendrobii Cordyceps fermentation original plasm wine, be prepared as follows and form:
(1) take Herba Dendrobii stem bar, remove crust, clean up, be cut into 0.6cm segment, by the weight ratio of Herba Dendrobii Yu water
Add the water purification that temperature is 95 DEG C, water temperature drop to 40 degree for 2:100, move into beater, beat by the speed of 1000 turns per minute
Starch 2 minutes, filter crude fibre, obtain Herba Dendrobii juice；
(2) the food grade cellulase preparation using enzyme activity to be 100,000 u/ml and pectase that enzyme activity is 50,000 u/ml
The ratio of the consumption of Herba Dendrobii juice, cellulase and pectase described in agents enzymolysis processing is 1:1, and the volume adding enzyme preparation accounts for
The 0.5% of Herba Dendrobii juice volume, hydrolysis temperature 50 DEG C, enzymolysis time is 45 minutes, filters dendrobium fiber and obtains enzymolysis Herba Dendrobii juice；
Scanning through visible ultraviolet light, in enzymolysis Herba Dendrobii juice, the maximum absorption band of alkaloid is 325um, and dendrobium total alkaloids contains
Amount 0.21mg/100ml, dendrobium polysaccharide content is 152mg/100ml；
(3) in described enzymolysis Herba Dendrobii juice, glucose 1.2g/100ml, sucrose 0.8g/100ml, soybean cake powder are added
1.0g/100ml and peptone 0.5g/100ml, sterilization treatment 20 minutes at temperature is 110 DEG C, obtain the fermentation of Herba Dendrobii juice Cordyceps
Culture fluid；
(4) from fresh natural cordyceps separation, purification the cordyceps species through molecular biology identification, press
The 0.5g/100ml amount following Cordyceps liquid seed culture medium of access: Rhizoma Solani tuber osi 20g/100ml, glucose 1.2g/100ml,
Peptone 0.6g/100ml, yeast extract 0.15g/100ml, surplus is water, is 22 degree in cultivation temperature, and rotary speed is 210
Rev/min, cultivate 46 hours, obtain Cordyceps liquid seeds；
Described Cordyceps seed liquor is moved in described Herba Dendrobii juice Cordyceps fermentation culture with 8ml/100ml, in fermentation
Temperature is 21 DEG C, and ratio (V/V) the 1:0.4 air agitation of liquid wind is fermented, and from sweat 36 hours, is often separated by 2 hours and uses iodine liquid
Off-line checks, to control the palliating degradation degree of dendrobium polysaccharide, when iodine liquid loss for reaction, terminates fermentation, obtains Herba Dendrobii juice Cordyceps and send out
Ferment oleo stock；
After testing, dendrobium total alkaloids content 0.20mg/100ml in described fermentation oleo stock, polysaccharide (Herba Dendrobii Cordyceps) content
163mg/100ml, Cordyceps adenosine content 0.74mg/100ml, cordycepin content 0.03 0mg/100ml, cordycepic acid 5.7 0mg/
100ml；
(5) by described Herba Dendrobii juice Cordyceps fermentation oleo stock input homogenizer, controlling homogenizer operation temperature is 45 DEG C, all
Matter pressure 90MPa, sends into high-pressure fluid nanometer mill from homogenizer effluent, controls nanometer mill operation temperature 78 DEG C, operates pressure
For 80MPa；
(6) Herba Dendrobii Cordyceps fermentation oleo stock is mixed according to volume ratio 1:1.2 with pure grain rice wine (alcoholic strength is 56 degree), old
Making temperature is 25 DEG C, and the aging time is 20 days, obtains Herba Dendrobii Cordyceps fermentation original plasm wine.
After testing, in the present embodiment gained Herba Dendrobii Cordyceps fermentation original plasm wine, dendrobium total alkaloids content 0.12mg/
100ml, polysaccharide (Herba Dendrobii Cordyceps) content is 81.0mg/100ml, Cordyceps adenosine content 0.34mg/100ml, cordycepin content
0.018mg/100ml, cordycepic acid 2.82mg/100ml.
Embodiment 2
Present embodiments provide a kind of Herba Dendrobii Cordyceps fermentation original plasm wine, be prepared as follows and form:
(1) take Herba Dendrobii stem bar, remove crust, clean up, be cut into 0.3cm segment, by the weight ratio of Herba Dendrobii Yu water
Add the water purification that temperature is 90 DEG C, water temperature drop to 45 degree for 3.5:100, move into beater, by the speed of 1000 turns per minute
Pull an oar 5 minutes, filter crude fibre, obtain Herba Dendrobii juice；
(2) the food grade cellulase preparation using enzyme activity to be 100,000 u/ml and pectase that enzyme activity is 50,000 u/ml
The amount ratio of Herba Dendrobii juice, cellulase and pectase described in agents enzymolysis processing is 1:1.5, and the volume adding enzyme preparation accounts for
The 0.6% of Herba Dendrobii juice volume, hydrolysis temperature 50 DEG C, enzymolysis time is 50 minutes, filters dendrobium fiber and obtains enzymolysis Herba Dendrobii juice；
Scanning through visible ultraviolet light, in enzymolysis Herba Dendrobii juice, the maximum absorption band of alkaloid is 325um, and dendrobium total alkaloids contains
Amount 0.285mg/100ml, dendrobium polysaccharide content is 283mg/100ml；
(3) in described enzymolysis Herba Dendrobii juice, glucose 1.2g/100ml, sucrose 0.8g/100ml, soybean cake powder are added
1.0g/100ml and peptone 0.5g/100ml, sterilization treatment 20 minutes at temperature is 110 DEG C, obtain the fermentation of Herba Dendrobii juice Cordyceps
Culture fluid；
(4) from fresh natural cordyceps separation, purification the cordyceps species identified, access by 0.5g/100ml amount
Following Cordyceps liquid seed culture medium: Rhizoma Solani tuber osi 20g/100ml, glucose 1.2g/100ml, peptone 0.6g/100ml,
Yeast extract 0.15g/100ml, surplus is water, is 22 degree in cultivation temperature, and rotary speed is 210 revs/min, cultivates 46 little
Time, obtain Cordyceps liquid seeds；
Described Cordyceps seed liquor is moved in described Herba Dendrobii juice Cordyceps fermentation culture with 10ml/100ml, in fermentation
Temperature is 23 DEG C, and ratio (V/V) the 1:0.5 air agitation of liquid wind is fermented, and from sweat 38 hours, is often separated by 2 hours and uses iodine liquid
Off-line checks, to control the palliating degradation degree of dendrobium polysaccharide, when iodine liquid loss for reaction, terminates fermentation, obtains Herba Dendrobii juice Cordyceps and send out
Ferment oleo stock；
After testing, total Herba Dendrobii alkaloid 0.276mg/100ml in described fermentation oleo stock, polysaccharide (stone in fermentation oleo stock
Dry measure used in former times Cordyceps) content 307mg/ml, Cordyceps adenosine content 0.685mg/100ml, cordycepin content 0.032mg/100ml, cordycepic acid
5.21mg/100ml；
(5) by described Herba Dendrobii juice Cordyceps fermentation oleo stock input homogenizer, controlling homogenizer operation temperature is 45 DEG C, homogenizing
Pressure 90MPa, sends into high-pressure fluid nanometer mill from homogenizer effluent, controls nanometer mill operation temperature 78 DEG C, and operation pressure is
120MPa；
(6) Herba Dendrobii Cordyceps fermentation oleo stock is mixed according to volume ratio 1:1, aging with pure grain rice wine (alcoholic strength is 62 degree)
Temperature is 25 DEG C, and the aging time is 20 days, obtains Herba Dendrobii Cordyceps fermentation original plasm wine.
After testing, in the present embodiment gained Herba Dendrobii Cordyceps fermentation original plasm wine, dendrobium total alkaloids content 0.131mg/
100ml, polysaccharide (Herba Dendrobii Cordyceps) content is 155.0mg/100ml, Cordyceps adenosine content 0.351mg/100ml, cordycepin content
0.014mg/100ml, cordycepic acid 2.62mg/100ml.
Embodiment 3
Present embodiments provide a kind of Herba Dendrobii Cordyceps fermentation original plasm wine, be prepared as follows and form:
(1) take Herba Dendrobii stem bar, remove crust, clean up, be cut into 0.6cm segment, by the weight ratio of Herba Dendrobii Yu water
Add the water purification that temperature is 95 DEG C, water temperature drop to 40 degree for 4:100, move into beater, beat by the speed of 1200 turns per minute
Starch 4 minutes, filter crude fibre, obtain Herba Dendrobii juice；
(2) the food grade cellulase preparation using enzyme activity to be 100,000 u/ml and pectase that enzyme activity is 50,000 u/ml
The amount ratio of Herba Dendrobii juice, cellulase and pectase described in agents enzymolysis processing is 1:2, and the volume adding enzyme preparation accounts for stone
The 0.8% of dry measure used in former times juice volume, hydrolysis temperature 55 DEG C, enzymolysis time is 55 minutes, filters dendrobium fiber and obtains enzymolysis Herba Dendrobii juice；
Scanning through visible ultraviolet light, in enzymolysis Herba Dendrobii juice, the maximum absorption band of alkaloid is 325um, and dendrobium total alkaloids contains
Amount 0.308mg/100ml, dendrobium polysaccharide content is 349mg/100ml；
(3) in described enzymolysis Herba Dendrobii juice, glucose 1.2g/100ml, sucrose 0.8g/100ml, soybean cake powder are added
1.0g/100ml and peptone 0.5g/100ml, sterilization treatment 20 minutes at temperature is 110 DEG C, obtain the fermentation of Herba Dendrobii juice Cordyceps
Culture fluid；
(4) from fresh natural cordyceps separation, purification the cordyceps species through molecular biology identification, press
The 0.5g/100ml amount following Cordyceps liquid seed culture medium of access: Rhizoma Solani tuber osi 20g/100ml, glucose 1.2g/100ml,
Peptone 0.6g/100ml, yeast extract 0.15g/100ml, surplus is water, is 22 degree in cultivation temperature, and rotary speed is 210
Rev/min, cultivate 46 hours, obtain Cordyceps liquid seeds；
Described Cordyceps seed liquor is moved in described Herba Dendrobii juice Cordyceps fermentation culture with 12ml/100ml, in fermentation
Temperature is 23 DEG C, and ratio (V/V) the 1:0.6 air agitation of liquid wind is fermented, and from sweat 42 hours, is often separated by 2 hours and uses iodine liquid
Off-line checks, to control the palliating degradation degree of dendrobium polysaccharide, when iodine liquid loss for reaction, terminates fermentation, obtains Herba Dendrobii juice Cordyceps and send out
Ferment oleo stock；
After testing, total Herba Dendrobii alkaloid 0.294mg/100ml in described fermentation oleo stock, polysaccharide (stone in fermentation oleo stock
Dry measure used in former times Cordyceps) content 362mg/100ml, Cordyceps adenosine content 0.801mg/100ml, cordycepin content 0.035mg/100ml, Cordyceps
Acid 4.97mg/100ml；
(5) by described Herba Dendrobii juice Cordyceps fermentation oleo stock input homogenizer, controlling homogenizer operation temperature is 45 DEG C, homogenizing
Pressure 90MPa, sends into high-pressure fluid nanometer mill from homogenizer effluent, controls nanometer mill operation temperature 85 DEG C, and operation pressure is
110MPa；
(6) Herba Dendrobii Cordyceps fermentation oleo stock is mixed according to volume ratio 1:1, aging with pure grain rice wine (alcoholic strength is 62 degree)
Temperature is 25 DEG C, and the aging time is 25 days, obtains Herba Dendrobii Cordyceps fermentation original plasm wine.
After testing, in the present embodiment gained Herba Dendrobii Cordyceps fermentation original plasm wine, dendrobium total alkaloids content 0.145mg/
100ml, polysaccharide (Herba Dendrobii Cordyceps) content is 186mg/100ml, Cordyceps adenosine content 0.260mg/100ml, cordycepin content
0.0170mg/100ml, cordycepic acid 2.91mg/100ml.
Embodiment 4
Present embodiments provide a kind of Herba Dendrobii Cordyceps fermentation original plasm wine, be prepared as follows and form:
(1) take Herba Dendrobii stem bar, remove crust, clean up, be cut into 0.5cm segment, by the weight ratio of Herba Dendrobii Yu water
Add the water purification that temperature is 95 DEG C, water temperature drop to 40 degree for 5:100, move into beater, beat by the speed of 1500 turns per minute
Starch 3 minutes, filter crude fibre, obtain Herba Dendrobii juice；
(2) the food grade cellulase preparation using enzyme activity to be 100,000 u/ml and pectase that enzyme activity is 50,000 u/ml
The amount ratio of Herba Dendrobii juice, cellulase and pectase described in agents enzymolysis processing is 1:2.5, and the volume adding enzyme preparation accounts for
The 1.0% of Herba Dendrobii juice volume, hydrolysis temperature 55 DEG C, enzymolysis time is 60 minutes, filters dendrobium fiber and obtains enzymolysis Herba Dendrobii juice；
Scanning through visible ultraviolet light, in enzymolysis Herba Dendrobii juice, the maximum absorption band of alkaloid is 325um, and dendrobium total alkaloids contains
Amount 0.388mg/100ml, dendrobium polysaccharide content is 439mg/100ml；
(3) in described enzymolysis Herba Dendrobii juice, glucose 1.2g/100ml, sucrose 0.8 g/100ml, soybean cake powder are added
1.0g/100ml and peptone 0.5g/100ml, sterilization treatment 20 minutes at temperature is 115 DEG C, obtain the fermentation of Herba Dendrobii juice Cordyceps
Culture fluid；
(4) from fresh natural cordyceps separation, purification the cordyceps species through molecular biology identification, press
The 0.5g/100ml amount following Cordyceps liquid seed culture medium of access: Rhizoma Solani tuber osi 20g/100ml, glucose 1.2g/100ml,
Peptone 0.6g/100ml, yeast extract 0.15g/100ml, surplus is water, is 22 degree in cultivation temperature, and rotary speed is 210
Rev/min, cultivate 46 hours, obtain Cordyceps liquid seeds；
Described Cordyceps seed liquor is moved in described Herba Dendrobii juice Cordyceps fermentation culture with 15ml/100ml, in fermentation
Temperature is 23 DEG C, and ratio (V/V) the 1:0.7 air agitation of liquid wind is fermented, and from sweat 44 hours, is often separated by 2 hours and uses iodine liquid
Off-line checks, to control the palliating degradation degree of dendrobium polysaccharide, when iodine liquid loss for reaction, terminates fermentation, obtains Herba Dendrobii juice Cordyceps and send out
Ferment oleo stock；
After testing, total Herba Dendrobii alkaloid 0.376mg/100ml in described fermentation oleo stock, polysaccharide (stone in fermentation oleo stock
Dry measure used in former times Cordyceps) content 453mg/100ml, Cordyceps adenosine content 0.724mg/100ml, cordycepin content 0.037mg/100ml, Cordyceps
Acid 6.03mg/100ml；
(5) by described Herba Dendrobii juice Cordyceps fermentation oleo stock input homogenizer, controlling homogenizer operation temperature is 45 DEG C, homogenizing
Pressure 90MPa, sends into high-pressure fluid nanometer mill from homogenizer effluent, controls nanometer mill operation temperature 85 DEG C, and operation pressure is
110MPa；
(6) Herba Dendrobii Cordyceps fermentation oleo stock is mixed according to volume ratio 1:1, aging with pure grain rice wine (alcoholic strength is 72 degree)
Temperature is 25 DEG C, and the aging time is 25 days, obtains Herba Dendrobii Cordyceps fermentation original plasm wine.
After testing, in the present embodiment gained Herba Dendrobii Cordyceps fermentation original plasm wine, dendrobium total alkaloids content is 0.381mg/
100ml, polysaccharide (Herba Dendrobii Cordyceps) content 481mg/100ml, Cordyceps adenosine content 0.359mg/100ml, cordycepin content
0.018mg/100ml, cordycepic acid 3.00mg/100ml.
Although, used general explanation, detailed description of the invention and test, the present invention made detailed retouching
Stating, but on the basis of the present invention, can make some modifications or improvements it, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Scope.
Claims (10)
1. the preparation method of a Herba Dendrobii Cordyceps fermentation original plasm wine, it is characterised in that comprise the following steps:
(1) stem of Dendrobium bar is pulled an oar, obtain Herba Dendrobii juice；
(2) use Herba Dendrobii juice described in cellulase and pectase associating enzymolysis processing, obtain enzymolysis Herba Dendrobii juice；
(3) in described enzymolysis Herba Dendrobii juice, add carbon source and nitrogen source, after sterilizing, obtain Herba Dendrobii juice Cordyceps fermentation culture；
(4) Cordyceps seed liquor subcultivation is entered described Herba Dendrobii juice Cordyceps fermentation culture, fermentation, obtain the fermentation of Herba Dendrobii juice Cordyceps
Oleo stock；
(5) by after described Herba Dendrobii juice Cordyceps fermentation oleo stock homogenizing, nano-level grinder is used to process；
(6) will mix with wine through the oleo stock after step (5) processes, after aging, obtain Herba Dendrobii juice Cordyceps fermentation original plasm wine.
Method the most according to claim 1, it is characterised in that described step (1) is particularly as follows: remove the peel stem of Dendrobium bar, Xian
Only, cutting, add the water of 90～95 DEG C with weight ratio 2～6:100, at a temperature for the treatment of, drop to 35～55 DEG C, at rotating speed 800～
Pull an oar 3～6 minutes under 1500rpm, obtain Herba Dendrobii juice.
Method the most according to claim 1 and 2, it is characterised in that in described step (2): in Herba Dendrobii juice described in every 100ml
Adding the work of total enzyme is 1 × 104U～25 × 104The cellulase of u and pectase carry out associating enzymolysis processing；The most described cellulose
The amount ratio of enzyme and pectase is 1:0.5～3；
Or/and, described enzymatic hydrolysis condition is: enzymolysis 0.5～2.5h at 40～60 DEG C.
4. according to the method described in claims 1 to 3 any one, it is characterised in that step (3) described addition carbon source and nitrogen source
Particularly as follows: add glucose 0.8～1.6g/100ml, sucrose 0.5～1.0g/100ml, soybean cake powder in described enzymolysis Herba Dendrobii juice
0.6～3.0g/100ml and peptone 0.5～1.0g/100ml；
And/or, described sterilizing particularly as follows: at 115～123 DEG C sterilization treatment 20～25 minutes.
5. according to the method described in Claims 1 to 4 any one, it is characterised in that described Cordyceps seed liquor is by fresh
Natural cordyceps in isolated and purified Cordyceps strain cultivate and obtain；
Preferably, described Cordyceps seed liquor is obtained by following methods: by the winter isolated and purified from fresh natural cordyceps
Worm summer grass strain, is seeded to Cordyceps liquid seed culture medium by the inoculum concentration of 0.5g/100ml, cultivates at temperature 22 DEG C
46 hours and obtain；
Consisting of of further preferred described Cordyceps liquid seed culture medium: Rhizoma Solani tuber osi 20g/100ml, glucose 1.2g/
100ml, peptone 0.6g/100ml, yeast extract 0.15g/100ml, surplus is water.
Method the most according to claim 5, it is characterised in that in Herba Dendrobii juice Cordyceps fermentation medium described in every 100ml,
Subcultivation enters Cordyceps seed liquor described in 6～15ml；
And/or, the condition of step (4) described fermentation is: air agitation fermentation at 19～25 DEG C, after 36 hours, is often separated by 2 little
Time pick up with iodine liquid off-line and to look into, to control the palliating degradation degree of dendrobium polysaccharide, when iodine liquid loss for reaction, terminate fermentation.
7. according to the method described in claim 1～6 any one, it is characterised in that described step (5) is particularly as follows: by described
Herba Dendrobii juice Cordyceps fermentation oleo stock inputs high-pressure fluid nanometer mill after homogenizing processes and processes；
The operation temperature of the most described high-pressure fluid nanometer mill is 75～90 DEG C, and operation pressure is 80～120MPa.
8. according to the method described in claim 1～7 any one, it is characterised in that in described step (6), described wine is wine
Precision 56～the pure grain rice wine of 72 degree；
And/or, described Herba Dendrobii Cordyceps fermentation oleo stock is 1:1～1.5 with the volume ratio of described wine；
And/or, the condition of described aging was: 20～35 DEG C of aging 15～30 days.
Method the most according to claim 1, it is characterised in that comprise the following steps:
(1) take Herba Dendrobii stem bar, remove crust, clean up, be cut into 0.3～0.6cm segment, by the weight ratio of Herba Dendrobii Yu water
It is 2～5:100 90～95 DEG C of water of addition, after water temperature drop to 40～45 DEG C, pulls an oar 3～5 points under rotating speed 1000～1500rpm
Clock, filters crude fibre, obtains Herba Dendrobii juice；
(2), in Herba Dendrobii juice described in every 100ml, adding the work of total enzyme is 1 × 104U～25 × 104The cellulase of u and pectase are carried out
Associating enzymolysis processing, the amount ratio of described cellulase and pectase is 1:0.5～3；Enzymolysis 40～60min at 55 DEG C, filter
Except dendrobium fiber, obtain enzymolysis Herba Dendrobii juice；
(3) in described enzymolysis Herba Dendrobii juice, glucose 1.2g/100ml, sucrose 0.8g/100ml, soybean cake powder 1.0g/100ml are added
And peptone 0.5g/100ml；Sterilization treatment 20min at 110～115 DEG C, obtains Herba Dendrobii juice Cordyceps fermentation culture；
(4) from fresh natural cordyceps separation, the Cordyceps strain of purification, access as follows by the amount of 0.5g/100ml
The Cordyceps liquid seed culture medium of composition: Rhizoma Solani tuber osi 20g/100ml, glucose 1.2g/100ml, peptone 0.6g/
100ml, yeast extract 0.15g/100ml, surplus is water；Cultivate 46 hours at temperature 22 DEG C, obtain Cordyceps seed liquor；
Described Cordyceps seed liquor is entered in described Herba Dendrobii juice Cordyceps fermentation culture with 8～15ml/100ml subcultivation, 21
～23 DEG C, air agitation fermentation under the conditions of liquid wind volume ratio 1:0.4～0.7, from sweat 36～44 hours, often it is separated by 2
Hour with iodine liquid off-line check, to control the palliating degradation degree of dendrobium polysaccharide, when iodine liquid loss for reaction, terminate fermentation, obtain Herba Dendrobii
Juice Cordyceps fermentation oleo stock；
(5) by described Herba Dendrobii juice Cordyceps fermentation oleo stock input homogenizer, 45 DEG C, carry out homogenizing, by homogenizing under the conditions of 90MPa
Machine effluent sends into high-pressure fluid nanometer mill, and control nanometer mill operation temperature 78～85 DEG C, operation pressure is 80～120MPa；
(6) by the Herba Dendrobii Cordyceps processed through step (5) fermentation oleo stock and alcoholic strength be the pure grain rice wine of 56～72 degree by volume
Ratio 1:1～1.2 mixing, aging 20～25 days at 25 DEG C, obtain Herba Dendrobii Cordyceps fermentation original plasm wine.
10. the Herba Dendrobii Cordyceps fermentation original plasm wine that method described in claim 1～9 any one is prepared from.
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