CN107267505B - 微卫星标记及其在结直肠癌的预后判定和/或化疗敏感性预测中的应用 - Google Patents
微卫星标记及其在结直肠癌的预后判定和/或化疗敏感性预测中的应用 Download PDFInfo
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CN201710599099.5A CN107267505B (zh) | 2017-07-21 | 2017-07-21 | 微卫星标记及其在结直肠癌的预后判定和/或化疗敏感性预测中的应用 |
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CN107937396A (zh) * | 2017-12-29 | 2018-04-20 | 北京莲和医学检验所有限公司 | 引物组、试剂盒以及同时检测单态性单核苷酸重复位点微卫星不稳定的方法 |
CN108866188B (zh) * | 2018-07-12 | 2022-03-01 | 吉林大学 | 一种消化道恶性肿瘤易感性预测试剂盒及*** |
CN108676891B (zh) * | 2018-07-12 | 2022-02-01 | 吉林大学 | 一种直肠腺癌易感性预测试剂盒及*** |
CN108676889B (zh) * | 2018-07-12 | 2022-02-01 | 吉林大学 | 一种胃腺癌易感性预测试剂盒及*** |
CN108676890B (zh) * | 2018-07-12 | 2022-01-28 | 吉林大学 | 一种女性乳腺恶性肿瘤易感性预测试剂盒及*** |
CN115094076B (zh) * | 2022-06-30 | 2023-05-16 | 斯贝福(北京)生物技术有限公司 | 一种用于体外检测致突变因子的报告质粒、细胞模型及其应用 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103555843A (zh) * | 2013-11-05 | 2014-02-05 | 上海赛安生物医药科技有限公司 | 结直肠癌微卫星不稳定性扩增体系及其检测试剂盒 |
CN104379765A (zh) * | 2012-04-10 | 2015-02-25 | 非营利性组织佛兰芒综合大学生物技术研究所 | 用于检测癌症中的微卫星不稳定性和测定与dna碱基切除修复途径抑制的合成致死性的新标记 |
CN106350596A (zh) * | 2016-10-17 | 2017-01-25 | 上海赛安生物医药科技有限公司 | 结直肠癌预后检测引物探针及其试剂盒 |
CN106498090A (zh) * | 2017-01-16 | 2017-03-15 | 上海普洛麦格生物产品有限公司 | 一种用于检测dna错配修复***的试剂盒及其用途 |
CN106755501A (zh) * | 2017-01-25 | 2017-05-31 | 广州燃石医学检验所有限公司 | 一种基于二代测序的同时检测微卫星位点稳定性和基因组变化的方法 |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104379765A (zh) * | 2012-04-10 | 2015-02-25 | 非营利性组织佛兰芒综合大学生物技术研究所 | 用于检测癌症中的微卫星不稳定性和测定与dna碱基切除修复途径抑制的合成致死性的新标记 |
CN103555843A (zh) * | 2013-11-05 | 2014-02-05 | 上海赛安生物医药科技有限公司 | 结直肠癌微卫星不稳定性扩增体系及其检测试剂盒 |
CN106350596A (zh) * | 2016-10-17 | 2017-01-25 | 上海赛安生物医药科技有限公司 | 结直肠癌预后检测引物探针及其试剂盒 |
CN106498090A (zh) * | 2017-01-16 | 2017-03-15 | 上海普洛麦格生物产品有限公司 | 一种用于检测dna错配修复***的试剂盒及其用途 |
CN106755501A (zh) * | 2017-01-25 | 2017-05-31 | 广州燃石医学检验所有限公司 | 一种基于二代测序的同时检测微卫星位点稳定性和基因组变化的方法 |
Non-Patent Citations (3)
Title |
---|
MSI-H/S结直肠癌的差异表达分子及MSI-H结直肠癌患者良好预后的潜在机制;吴艳峰;《第九届全国免疫学学术大会论文集》;20141231;摘要 * |
检测微卫星不稳在中国遗传性非息肉病性结直肠癌患者诊断中的应用;徐烨;《中国癌症杂志》;20060316;第16卷(第2期);第1-4页 * |
结直肠腺瘤中的微卫星不稳定性类型;程蕾;《中国生物化学与分子生物学报》;20031231;第19卷(第6期);第799-805页 * |
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