CN107245430A - A kind of cell 3D printing system and Method of printing - Google Patents
A kind of cell 3D printing system and Method of printing Download PDFInfo
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- CN107245430A CN107245430A CN201710488481.9A CN201710488481A CN107245430A CN 107245430 A CN107245430 A CN 107245430A CN 201710488481 A CN201710488481 A CN 201710488481A CN 107245430 A CN107245430 A CN 107245430A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
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Abstract
The present invention provides a kind of cell 3D printing system and Method of printing.A kind of cell 3D printing system; wherein; including constant temperature cabin and the barrel being located in the constant temperature cabin; the constant temperature bilge portion is provided with nozzle system; the barrel is connected with the nozzle system; the nozzle system is provided with when being used for the nozzle system ejection celliferous drop of bag, and the air-stream generating device of air-flow protection layer is formed between nozzle system and the celliferous drop of bag.The present invention is simple in construction, easy to use, can effectively reduce the damage that print procedure is likely to result in cytoactive, it is possible to further realize unicellular 3D printing, and improve flexibility and the shaping efficiency of shaping.
Description
Technical field
The present invention relates to bioengineered tissue technical field, more particularly, to a kind of cell 3D printing system and printing
Method.
Background technology
Organizational project is the subjects such as fusion engineering science, life science and material science, by imitating human tissue organ's shape
Into process build and cultivate in vitro the structure with bioactivity.Among these, 3D printing technique is because it is usable more
Kind of material is molded any complicated three-dimensional structure, as field of tissue engineering technology most strong research meanses.The original of 3D printing technique
Reason is exactly Layered manufacturing, is accumulated layer by layer.Traditional cell printing shower nozzle is exactly after cell is mixed with biomaterial and extruded shape
Into a silk thread shape, face is formed repeatedly reciprocal, the accumulation in face then forms corresponding three-dimensional structure.Typical shower nozzle among these
It is exactly electric motor boosted formula shower nozzle.
Fig. 1 is traditional electric motor boosted formula shower nozzle three-dimensional structure subdivision schematic diagram, and linear stepping motor is fixed on sportswear
Put on support, screw rod can apply certain pressure under the drive of linear stepping motor to the moulding material in shower nozzle, shape material
Material is extruded from nozzle immediately, and heating rod and insulated sheath are arranged on the temperature that shower nozzle hypomere makes the moulding material in shower nozzle keep setting
Degree.
Fig. 2 is conventional motors assisted shower nozzle extrusion effect, and Fig. 3 is traditional sprinkler heads extruded material cross-sectional view, from
It can be seen that cell is included in biomaterial extrudes in figure, multiple cells are extruded simultaneously, and cell is wherein
Position is random uncontrollable.Cell is not protected in extrusion, is influenceed by the shearing force that nozzle inside diameter is produced, meeting
Cause the phenomenons such as membranolysis death to produce, greatly reduce the activity of cell.
Therefore, although such a design is simple and reliable, also there is following weak point：1) formed precision is not high to realize
For single celled injection, the minimum internal diameter of traditional prints shower nozzle, which is 150 μm or so, is far longer than the size of 10 μm of cell dia,
Multiple cells can be extruded simultaneously, position of the cell in formed body is random uncontrollable；2) in extrusion, cell can be by
The influence for the shearing force that nozzle interior is produced so that cytoactive sustains damage；3) speed of extrusion is relatively low, and the efficiency of shaping is not
It is high.
The content of the invention
There is provided a kind of cell 3D printing system at least one defect for overcoming described in above-mentioned prior art by the present invention.Should
3D printing system architecture is simple, easy to use.The damage that print procedure is likely to result in cytoactive can be reduced, further
Ground, it is possible to achieve unicellular 3D printing, and improve flexibility and the shaping efficiency of shaping.
It is a further object to provide a kind of cell 3D printing method.
In order to solve the above technical problems, the technical solution adopted by the present invention is：A kind of cell 3D printing system, wherein, bag
Include constant temperature cabin and the barrel being located in the constant temperature cabin, the constant temperature bilge portion is provided with nozzle system, the barrel with it is described
Nozzle system is connected, and the nozzle system is provided with when being used for nozzle system ejection cell, the shape between nozzle system and cell
Into the air-stream generating device of air-flow protection layer.Cell suspending liquid is contained in barrel, and constant temperature cabin can be provided to cell suspending liquid
One isoperibol, it is to avoid because outside environmental elements change and caused by cytoactive damage；Small part cell in barrel suspends
Liquid enters nozzle system, and is sprayed from nozzle system with wrapping celliferous drops, and phase is finally arranged out on bottom plate
The shape answered, nozzle system is when spraying the celliferous drop of bag, and air-stream generating device is wrapping celliferous drop and nozzle system
One layer of air-flow protection layer is generated between the nozzle inner walls of system, protective effect is served, reduces the celliferous drop of bag and nozzle system
Shearing force between the nozzle inner walls of system, improves cell survival rate.
Further, the nozzle system includes inner nozzle, outer nozzle and is located at the inner nozzle rear end for extruding
The drive device of celliferous drop is wrapped, the outer nozzle is set in the front end of the inner nozzle, and the cell of injection sprays from nozzle
The one end gone out is the front end of nozzle, spaced apart between outer nozzle inwall and inner nozzle outer wall；The air-stream generating device
Including the air inlet pipe being located on the outer wall of the outer nozzle rear end, and the air guide being oppositely arranged on the outer wall of the outer nozzle front end
Pipe, the airway tube is tilted towards the front end of the outer nozzle, between the central axis of airway tube and the central axis of outer nozzle
Angle be 30 ~ 60 °, it is preferred that the angle between the central axis of the airway tube and the central axis of outer nozzle is 45 °.
The effect of inner nozzle is to eject the celliferous drop of bag, and gases at high pressure are passed through in the air inlet pipe of outer nozzle, and in outer nozzle
Airway tube on apply the stable air-flow sprayed downwards be formed in the middle of suction force, such inner nozzle and outer nozzle, when comprising
When single celled drop is ejected into outer nozzle, wrapping between celliferous drop and outer nozzle inwall just has one layer of air cushion, serves
Protective effect, reduces the shearing force between the celliferous drop of bag and outer nozzle inwall, improves cell survival rate, gas along
When the inwall of outer nozzle is ejected into the front end of outer nozzle, most air-flows will be by the airway tube row positioned at outer nozzle front end
Go out, it is to avoid influence the direction of motion of the celliferous drop of bag ejected from outer nozzle.Airway tube is towards the outer nozzle
Front end, which is tilted, can ensure that most air-flows aspirate discharge by airway tube.
Further, the barrel is connected with the nozzle system by pipeline, between barrel and the nozzle system
Pipeline is provided with check valve.Cell suspending liquid in barrel, small part via enter inner nozzle, check valve after check valve can be with
Prevent cell suspending liquid from flowing back.
Further, the internal diameter of the inner nozzle is more than the diameter of individual cells, and less than 150 μm, it is preferred that in described
The internal diameter of nozzle is 20 ~ 90 μm, and the internal diameter of outer nozzle is 100 ~ 200 μm.So, the internal diameter of inner nozzle is slightly larger than the diameter of cell
10 μm, much smaller than 150 μm of the minimum diameter of traditional 3D printing shower nozzle, to ensure that including for drive device driving inner nozzle ejection is thin
It is only unicellular comprising one in the drop of born of the same parents, so as to realize single celled 3D printing.
Further, the drive device includes being located at the driving diaphragm of the inner nozzle rear end, front end and the driving
Push rod that diaphragm is close to and the piezoelectric ceramics for being connected to the putting back-end.Piezoelectric ceramics becomes under the stimulation of pulse voltage
Push rod below shape, promotion, and then driving diaphragm deformation is promoted, driving diaphragm is deformed downwards, and the volume inside inner nozzle diminishes
Pressure increases and then extrudes a celliferous drop of bag；Closed check valve when extruding drop, drop is extruded from inner nozzle, works as pressure
When electroceramics back shrinkage drives driving diaphragm upwardly-deformed, inner nozzle internal pressure reduces, and check valve is opened, and is stored in barrel
In cell suspension enter inner nozzle along pipeline, complete a circulation.Piezoelectric ceramics is controllable under the control of pulse voltage
Deform and then promote the ejection of drop one by one.
Further, it is sequentially provided with below the outer nozzle for making from electric charge on the cell band of outer nozzle ejection
Polarized electric field, and for controlling the cell for taking electric charge to arrange out the deflecting electric fields of respective shapes on bottom plate.Nozzle system
The cell that system sprays takes electric charge in the presence of polarized electric field, and the then control through deflecting electric field is arranged out accordingly on bottom plate
Shape.
The present invention also provides a kind of cell 3D printing method, more specifically, being a kind of unicellular 3D printing method, using upper
The progress of cell 3D printing system is stated, is comprised the following steps：
S1. select the cell and biomaterial needed, according to suitable proportions, cell suspending liquid is made, load barrel standby
With；
S2. constant temperature cabin, polarized electric field and deflecting electric field are opened, gases at high pressure are passed through to air inlet pipe, and applies on airway tube and takes out
Suction so that the stable air-flow sprayed downwards is formed in the middle of inner nozzle and outer nozzle；
S3. the cell suspending liquid in barrel, enters inner nozzle through check valve, starts drive device, by cell suspending liquid with one
The form of individual drop microballoon extrudes into outer nozzle from inner nozzle, is then sprayed from outer nozzle；
S4. spray the celliferous drop microballoon of bag from outer nozzle and enter polarized electric field, wrap celliferous drop microballoon in polarization electricity
Electric charge is taken in the presence of；
S5. the celliferous drop microballoon of bag for taking electric charge enters deflecting electric field, under the control of deflecting electric field, on bottom plate
Arrange out corresponding shape.
Further, in step S1, the cell is fat stem cell, marrow fills interstital stem cell, cardiac muscle cell, Xue Wang
Any one in cell, liver cell and cancer cell etc..In step S1, the biomaterial is fibrinogen, collagen, bright
In glue, fibroin, alginates, hyaluronic acid and chitosan etc. any one or it is two or more.
Compared with prior art, the present invention has the advantages that：
The cell 3D printing system of the present invention adds air-flow protection on nozzle system so that the celliferous drop of bag and nozzle
One layer of air cushion is formed between inwall, weakens and is even a cancellation adversely affecting for the shearing force that cell is subject to, is substantially increased thin
Born of the same parents' survival rate.The inner nozzle for the cell 3D printing system that the present invention is provided and the internal diameter of outer nozzle are all smaller, improve cell into
Type precision, realizes and is directed to single celled 3D printing.Traditional prints shower nozzle, because nozzle inside diameter is larger, is the quilt of multiple cells simultaneously
Extrusion, and position of the cell in extruded material is random uncontrollable.Meanwhile, 3D printing system of the invention uses piezoelectricity
Ceramics spray unicellular to drive, and the arrangement of cell is controlled along with electric field, and all electronic control, shaping efficiency is high.
Brief description of the drawings
Fig. 1 is conventional motors assisted shower nozzle three-dimensional structure subdivision schematic diagram.
Fig. 2 is conventional motors assisted shower nozzle extruded material design sketch.
Fig. 3 is conventional motors assisted shower nozzle extruded material cross-sectional view.
Fig. 4 is the overall structure diagram of the present invention.
In figure：201-screw rod；202-linear stepping motor；203-heating rod；204-insulated sheath；205-nozzle；
401-cell；402-biomaterial；1-constant temperature cabin；2-barrel；3-inner nozzle；4-outer nozzle；5-air inlet pipe；6-lead
Tracheae；7-check valve；8-driving diaphragm；9-push rod；10-piezoelectric ceramics；11-polarized electric field；12-deflecting electric field.
Embodiment
Accompanying drawing being given for example only property explanation, it is impossible to be interpreted as the limitation to this patent；It is attached in order to more preferably illustrate the present embodiment
Scheme some parts to have omission, zoom in or out, do not represent the size of actual product；To those skilled in the art,
Some known features and its explanation may be omitted and will be understood by accompanying drawing.Being given for example only property of position relationship described in accompanying drawing
Explanation, it is impossible to be interpreted as the limitation to this patent.
Embodiment 1
As shown in figure 4, a kind of cell 3D printing system, wherein, including constant temperature cabin 1 and the barrel 2 that is located in constant temperature cabin 1, it is permanent
The warm bottom of cabin 1 is provided with nozzle system, and barrel 2 is connected with nozzle system, and nozzle system, which is provided with, is used for nozzle system ejection cell
When, the air-stream generating device of air-flow protection layer is formed between nozzle system and cell.Cell suspending liquid is contained in barrel 2,
Constant temperature cabin 1 can give cell suspending liquid provide an isoperibol, it is to avoid because outside environmental elements change and caused by cytoactive
Damage；Small part cell suspending liquid in barrel 2 enters nozzle system, and to wrap celliferous drop from nozzle system
Form sprays, and corresponding shape is finally arranged out on bottom plate, nozzle system is when spraying the celliferous drop of bag, and air-flow occurs
Device is wrapping one layer of air-flow protection layer of generation between celliferous drop and the nozzle inner walls of nozzle system, serves protection and makees
With the shearing force between the celliferous drop of reduction bag and the nozzle inner walls of nozzle system improves cell survival rate.
As shown in figure 4, nozzle system includes inner nozzle 3, outer nozzle 4 and is located at the rear end of inner nozzle 3 for extruding
The drive device of celliferous drop is wrapped, outer nozzle 4 is set in the front end of inner nozzle 3, the inwall of outer nozzle 4 and the outer wall of inner nozzle 3
Between it is spaced apart；Air-stream generating device includes the air inlet pipe 5 being located on the rear end outer wall of outer nozzle 4, and is oppositely arranged
Airway tube 6 on the front end outer wall of outer nozzle 4, airway tube 6 towards outer nozzle 4 front end tilt, the central axis of airway tube 6 with
Angle between the central axis of outer nozzle 4 is 45 °.The effect of inner nozzle 3 is to eject the celliferous drop of bag, in outer nozzle
Gases at high pressure are passed through in 4 air inlet pipe 5, and apply on the airway tube 6 of outer nozzle 4 suction force, such inner nozzle 3 and outer nozzle
The stable air-flow sprayed downwards is formed in the middle of 4, when the celliferous drop of bag is ejected into outer nozzle 4, celliferous liquid is wrapped
Just there is one layer of air cushion between drop and the inwall of outer nozzle 4, serve protective effect, reduce in the celliferous drop of bag and outer nozzle 4
Shearing force between wall, improves cell survival rate, when gas is ejected into the front end of outer nozzle 4 along the inwall of outer nozzle 4, absolutely
Most of air-flow will be discharged by the airway tube 6 positioned at the front end of outer nozzle 4, it is to avoid be influenceed from including that outer nozzle 4 is ejected
The direction of motion of the drop of cell.Airway tube 6 is tilted towards the front end of outer nozzle 4 can ensure most air-flows by airway tube
6 suction discharges.
As shown in figure 4, barrel 2 is connected with nozzle system by pipeline, the pipeline between barrel 2 and nozzle system is provided with
Check valve 7.Cell suspending liquid in barrel 2, small part can be prevented via inner nozzle 3, check valve 7 is entered after check valve 7
Cell suspending liquid flows back.
In the present embodiment, the internal diameter of inner nozzle 3 is 20 ~ 90 μm, and the internal diameter of outer nozzle 4 is 100 ~ 200 μm.So, interior spray
The internal diameter of mouth 3 is slightly larger than 10 μm of the diameter of cell, much smaller than 150 μm of the minimum diameter of traditional 3D printing shower nozzle, to ensure driving
It is only unicellular comprising one in the celliferous drop of bag that device driving inner nozzle 3 sprays, so as to realize single celled 3D printing.
As shown in figure 4, drive device includes being located at the driving diaphragm 8 of the rear end of inner nozzle 3, front end with driving diaphragm 8 to be close to
Push rod 9 and be connected to the piezoelectric ceramics 10 of the rear end of push rod 9.Piezoelectric ceramics 10 is deformed under the stimulation of pulse voltage, is promoted
The push rod 9 of lower section, and then promote driving diaphragm 8 to deform, driving diaphragm 8 is deformed downwards, and the volume inside inner nozzle 3 diminishes pressure
Increase and then extrude a celliferous drop of bag.Check valve 7 is closed during extrusion drop, and drop is extruded from inner nozzle 3, works as piezoelectricity
When ceramic 10 back shrinkages drive driving diaphragm 8 upwardly-deformed, the internal pressure of inner nozzle 3 reduces, and check valve 7 is opened, is stored in
Cell suspension in barrel 2 enters inner nozzle 3 along pipeline, completes a circulation.Control of the piezoelectric ceramics 10 in pulse voltage
Under controllable deformation and then promote the ejection of drop one by one.
As shown in figure 4, the lower section of outer nozzle 4 is sequentially provided with for making the pole from electric charge on the cell band of the ejection of outer nozzle 4
Change electric field 11, and for controlling the cell for taking electric charge to arrange out the deflecting electric field 12 of respective shapes on bottom plate.Nozzle system
The cell that system sprays takes electric charge in the presence of polarized electric field 11, and the control then through deflecting electric field 12 is arranged out on bottom plate
Corresponding shape.
Embodiment 2
The present embodiment is the Method of printing of the unicellular 3D printing system described in embodiment 1, wherein, comprise the following steps：
S1. fat stem cell and fibrinogen solution are selected, wherein, fibrinogen solution is by fibrinogen powder
It is dissolved in DMEM solution, mass concentration is 0.1%（w/v）, fat stem cell is mixed into fibrinogen solution, density is 1
×106Individual/mL, adds endothelial growth factor thereto（50ng/mL）, cell suspending liquid is made, loads barrel 2 standby；
S2. constant temperature cabin 1, polarized electric field 11 and deflecting electric field 12 are opened, gases at high pressure are passed through to air inlet pipe 5, and in airway tube 6
Upper application suction force so that the stable air-flow sprayed downwards is formed in the middle of inner nozzle 3 and outer nozzle 4；
S3. the cell suspending liquid in barrel 2, enters inner nozzle 3 through check valve 7, starts drive device, by cell suspending liquid with
The form of drop microballoon extrudes into outer nozzle 4 from inner nozzle 3 one by one, is then sprayed from outer nozzle 4；
S4. spray the celliferous drop microballoon of bag from outer nozzle 4 and enter polarized electric field 11, wrap celliferous drop microballoon in pole
Electric charge is taken in the presence of change electric field 11；
S5. the celliferous drop microballoon of bag for taking electric charge enters deflecting electric field 12, under the control of deflecting electric field 12, the bottom of at
Corresponding shape is arranged out on plate.
Certainly, the cell that the present embodiment is used can also be that marrow fills interstital stem cell, cardiac muscle cell, schwann cell, liver
The species such as cell and cancer cell, biological natural macromolecular material can also be used collagen, gelatin, fibroin, alginates, hyaluronic acid and
Chitosan etc..
Obviously, the above embodiment of the present invention is just for the sake of clearly demonstrating example of the present invention, and is not
Restriction to embodiments of the present invention.For those of ordinary skill in the field, on the basis of the above description also
It can make other changes in different forms.There is no necessity and possibility to exhaust all the enbodiments.It is all
Any modifications, equivalent substitutions and improvements made within the spirit and principles in the present invention etc., should be included in right of the present invention will
Within the protection domain asked.
Claims (12)
1. a kind of cell 3D printing system, it is characterised in that including constant temperature cabin（1）And it is located at the constant temperature cabin（1）Interior material
Cylinder（2）, the constant temperature cabin（1）Bottom is provided with nozzle system, the barrel（2）Connected with the nozzle system, the nozzle system
System is provided with when being used for nozzle system ejection cell, and the air-flow that air-flow protection layer is formed between nozzle system and cell is filled
Put.
2. a kind of cell 3D printing system according to claim 1, it is characterised in that the nozzle system includes inner nozzle
（3）, outer nozzle（4）And it is located at the inner nozzle（3）Rear end is used for the drive device for extruding the celliferous drop of bag, described outer
Nozzle（4）It is set in the inner nozzle（3）Front end, outer nozzle（4）Inwall and inner nozzle（3）A spacing is spaced between outer wall
From；The air-stream generating device includes being located at the outer nozzle（4）Air inlet pipe on the outer wall of rear end（5）, and be oppositely arranged on
The outer nozzle（4）Airway tube on the outer wall of front end（6）, the airway tube（6）Towards the outer nozzle（4）Front end tilt.
3. a kind of cell 3D printing system according to claim 2, it is characterised in that the airway tube（6）Central shaft
Line and the outer nozzle（4）Central axis between angle be 30 ~ 60 °.
4. a kind of cell 3D printing system according to claim 3, it is characterised in that the airway tube（6）Central shaft
Line and outer nozzle（4）Central axis between angle be 45 °.
5. a kind of cell 3D printing system according to claim 2, it is characterised in that the inner nozzle（3）Internal diameter it is big
In the diameter of individual cells, and less than 150 μm.
6. a kind of cell 3D printing system according to claim 5, it is characterised in that the inner nozzle（3）Internal diameter be
20 ~ 90 μm, outer nozzle（4）Internal diameter be 100 ~ 200 μm.
7. a kind of cell 3D printing system according to claim 5, it is characterised in that the drive device includes being located at institute
State inner nozzle（3）The driving diaphragm of rear end（8）, front end and the driving diaphragm（8）The push rod being close to（9）And be connected to described
Push rod（9）The piezoelectric ceramics of rear end（10）.
8. a kind of cell 3D printing system according to claim any one of 1-7, it is characterised in that the barrel（2）With
The nozzle system is connected by pipeline, barrel（2）Pipeline between the nozzle system is provided with check valve（7）.
9. a kind of cell 3D printing system according to claim any one of 2-7, it is characterised in that the nozzle system
Lower section is sequentially provided with for making from the outer nozzle（4）The polarized electric field of electric charge on the cell band of ejection（11）, and for controlling
The cell that system takes electric charge arranges out the deflecting electric fields of respective shapes on bottom plate（12）.
10. a kind of cell 3D printing method, it is characterised in that comprise the following steps：
S1. select the cell and biomaterial needed, according to suitable proportions, cell suspending liquid is made, load barrel
（2）It is standby；
S2. constant temperature cabin is opened（1）, polarized electric field（11）And deflecting electric field（12）, to air inlet pipe（5）It is passed through gases at high pressure, and
Airway tube（6）Upper application suction force so that inner nozzle（3）And outer nozzle（4）Centre forms the stable air-flow sprayed downwards；
S3. barrel（2）In cell suspending liquid, through check valve（7）Into inner nozzle（3）, start drive device, cell hanged
Supernatant liquid is in the form of drop microballoon one by one from inner nozzle（3）Extrude into outer nozzle（4）, then from outer nozzle（4）Spray；
S4. from outer nozzle（4）Spray the celliferous drop microballoon of bag and enter polarized electric field（11）, wrap celliferous drop microballoon
In polarized electric field（11）In the presence of take electric charge；
S5. the celliferous drop microballoon of bag for taking electric charge enters deflecting electric field（12）, in deflecting electric field（12）Control under,
Corresponding shape is arranged out on bottom plate.
11. a kind of cell 3D printing method according to claim 10, it is characterised in that in step S1, the cell is
Fat stem cell, marrow fill any one in interstital stem cell, cardiac muscle cell, schwann cell, liver cell and cancer cell.
12. a kind of cell 3D printing method according to claim 10, it is characterised in that in step S1, the biological material
Expect for any one in fibrinogen, collagen, gelatin, fibroin, alginates, hyaluronic acid and chitosan or two or more.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710488481.9A CN107245430A (en) | 2017-06-23 | 2017-06-23 | A kind of cell 3D printing system and Method of printing |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710488481.9A CN107245430A (en) | 2017-06-23 | 2017-06-23 | A kind of cell 3D printing system and Method of printing |
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| CN201710488481.9A Withdrawn CN107245430A (en) | 2017-06-23 | 2017-06-23 | A kind of cell 3D printing system and Method of printing |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107962770A (en) * | 2017-12-18 | 2018-04-27 | 中国科学院沈阳自动化研究所 | A kind of biological 3D printing system based on Coulter principle |
| CN107984743A (en) * | 2017-11-29 | 2018-05-04 | 中国科学院沈阳自动化研究所 | A kind of biology 3D printing system |
| CN110229740A (en) * | 2019-06-14 | 2019-09-13 | 科先医疗科技(苏州)有限公司 | A kind of Biotype artificial liver cell 3D printing system |
| CN111073607A (en) * | 2020-03-05 | 2020-04-28 | 郑州轻工业大学 | Phase change sphere preparation system and control method thereof |
-
2017
- 2017-06-23 CN CN201710488481.9A patent/CN107245430A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107984743A (en) * | 2017-11-29 | 2018-05-04 | 中国科学院沈阳自动化研究所 | A kind of biology 3D printing system |
| CN107962770A (en) * | 2017-12-18 | 2018-04-27 | 中国科学院沈阳自动化研究所 | A kind of biological 3D printing system based on Coulter principle |
| CN110229740A (en) * | 2019-06-14 | 2019-09-13 | 科先医疗科技(苏州)有限公司 | A kind of Biotype artificial liver cell 3D printing system |
| CN111073607A (en) * | 2020-03-05 | 2020-04-28 | 郑州轻工业大学 | Phase change sphere preparation system and control method thereof |
| CN111073607B (en) * | 2020-03-05 | 2023-12-12 | 郑州轻工业大学 | Phase-change sphere preparation system and control method thereof |
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