CN107233241A - A kind of preparation method and application of the emulsion containing hill gooseberry - Google Patents
A kind of preparation method and application of the emulsion containing hill gooseberry Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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- A61K8/46—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract
The invention discloses a kind of preparation method and application of emulsion containing hill gooseberry, its preparation method is that the aqueous solution for being formulated the extract of fresh hill gooseberry's ripening fruits and Portugal peach fruit with lauryl sodium sulfate or stearic acid or combination is mixed, after being sufficiently stirred for, finished product is obtained.And the emulsion is used to suppress in plant pathogenic fungi and whitening sun protection cosmetics, and the lytic agent combined using methanol and chloroform is fully extracted, and the extract of hill gooseberry and Portugal peach is fully extracted active compound and not oxidized.The emulsion that the preparation method is obtained is directly used in suppression plant pathogenic fungi, it is to avoid the use pollution on the environments of other chemical substance agricultural chemicals, crops are high to its bioavailability, and effect is notable；Extensively, environmental protection is degradable for raw material sources.
Description
Technical field
The present invention relates to the preparation method and application of technical field of pesticide, more particularly to a kind of emulsion containing hill gooseberry.
Background technology
The preventing and treating of current plant pathogenic fungi is a large amount of to use chemical pesticide to ecology based on the chemical agent of organic synthesis
Environment affects, therefore screens new Substance from plant and develop now in nuisanceless bactericide
One of medicine research direction.There are phenols, flavonoids and the Anthraquinones such as abundant phloroglucin etc. in hill gooseberry's fruit to plant
Pathogen has the composition of better inhibition effect.But aldehydes matter is oxidized easily in atmosphere, exposure in atmosphere can cause
It is oxidized and causes structure to occur isomerization and cause to go bad.Stability of this kind of compound in hill gooseberry's plant cell is given
We point out, and aldehydes matter are extracted using oil-in-water surface active agent emulsions, so as to avoid aldehydes matter and air
Contact.This extracting mode, can greatly improve the effective rate of utilization of aldehydes matter.
The content of the invention
In view of this, the invention provides a kind of preparation method of emulsion containing hill gooseberry, the extraction fully extracted is realized
Thing effectively suppresses the effect of disease fungus and whitening sun protection.
The technological means that the present invention is used is as follows：A kind of preparation method of emulsion containing hill gooseberry, comprises the following steps：
S1, myrtle fruit and Portugal peach fruit is clean, dries pulverizing；
S2, extracted with methanol solution, evaporation solvent obtains filter residue, then dissolves filter residue with chloroform, and filtering obtains the first filter residue
And filtrate；
S3, step S2 the first filter residue methanol dissolved, vacuum distillation solvent obtains the first extract, and filtrate is subtracted
Pressure distillation, obtains the second extract, merges the first extract and the second extract, and adds lauryl sodium sulfate, obtains liquid liquid
Phase；
S4, at least one of lauryl sodium sulfate or stearic acid be configured to the aqueous solution；
S5, by step the S4 aqueous solution and deionized water in mass ratio be 0.1~0.3:1 ratio is weighed, and is heated to 60
~70 DEG C, obtain mixed liquor；
S6, in mass ratio be 0.5~1.5:The mixed liquor that 1~2 ratio prepares step S5 is added in S3 liquid liquid phases,
High-speed stirred, obtains emulsion；
S7, by emulsion be incubated 15~20min, cooling regulation pH value to 4.5~6.5, obtain finished product.
Preferably, myrtle fruit and the mass ratio of Portugal peach fruit are 3 in the step S1:1~5:1.
Preferably, in the step S2, with the methanol solution that volume fraction is 60~80% in shaking bath 45 DEG C~60
1~2h of mechanical shaking extraction at DEG C.
Preferably, the mass concentration of liquid liquid phase is 0.1~10% in the step S3.
Preferably, lauryl sodium sulfate or stearic mass concentration are 0.1~15% in the step S4.
Preferably, it is 0.5~0.8 in mass ratio in the step S6:The mixing that 1~1.2 ratio prepares step S5
Liquid is added in S3 liquid liquid phases.
Preferably, in 20000 revs/min of lower uniform stirrings at a high speed in the step S6.
Preferably, pH value is adjusted when being cooled to 30~40 DEG C in the step S7.
Present invention also offers a kind of application of emulsion containing hill gooseberry in plant pathogenic fungi and whitening sun protection.
It is easily operated in its preparation process using a kind of preparation method of emulsion containing hill gooseberry provided by the present invention.It is logical
The combined use for crossing methanol and chloroform is extracted to myrtle fruit and Portugal peach fruit, and by dissolving extraction repeatedly, is fully extracted
Go out phenols, flavonoids and Anthraquinones various active chemical composition；Lauryl sodium sulfate is added into extract, liquid is obtained
Liquid phase, pre-emulsification extract, makes extract not oxidizable；Lauryl sodium sulfate or stearic acid or combine be configured to stability compared with
Good mixed liquor so that the effect that mixed liquor is further emulsified to primary emulsifying extract, the finished product of preparation is fully right
Phenolic compound, ketone etc. have protective effect, make its not oxidation by air, in the preventing and treating and whitening for plant pathogenic fungi
In sun care preparations, the effect with good curative effect, with stability.This process simplify intermediate steps, make preparation process more
Increase effect and environmental protection, cost is also accordingly reduced.
Embodiment
The principles and features of the present invention are described below, and the given examples are served only to explain the present invention, is not intended to limit
Determine the scope of the present invention.
Embodiment 1
A kind of preparation method of emulsion containing hill gooseberry, comprises the following steps：
S1,300g myrtle fruits and 100g Portugals peach fruit is clean, dries pulverizing.
S2, with volume fraction for 60% methanol solution at 45 DEG C of shaking bath mechanical shaking extraction 1h.Evaporation solvent must be filtered
Slag, then filter residue is dissolved with chloroform, filtering obtains the first filter residue and filtrate.
S3, step S2 the first filter residue methanol dissolved, vacuum distillation solvent obtains the first extract, and filtrate is subtracted
Pressure distillation, obtains the second extract, merges the first extract and the second extract, and adds lauryl sodium sulfate, obtains quality dense
Spend the liquid liquid phase for 0.1%.
S4, mass concentration for 0.1% lauryl sodium sulfate is configured to the aqueous solution.
S5, by step the S4 aqueous solution and deionized water in mass ratio be 0.1:1 ratio is weighed, and is heated to 60 DEG C, is obtained
Mixed liquor.
S6, in mass ratio be 0.5:The mixed liquor that 1 ratio prepares step S5 is added in S3 liquid liquid phases, at a high speed
20000 revs/min of lower uniform stirrings, obtain emulsion.
S7, emulsion is incubated 15min, regulation pH value obtains finished product to 4.5 when being cooled to 30 DEG C.
Application of the present invention also in plant pathogenic fungi and whitening sun protection.
Embodiment 2
A kind of preparation method of emulsion containing hill gooseberry, comprises the following steps：
S1,500g myrtle fruits and 100g Portugals peach fruit is clean, dries pulverizing.
S2, with volume fraction for 80% methanol solution at 60 DEG C of shaking bath mechanical shaking extraction 2h.Evaporation solvent must be filtered
Slag, then filter residue is dissolved with chloroform, filtering obtains the first filter residue and filtrate.
S3, step S2 the first filter residue methanol dissolved, vacuum distillation solvent obtains the first extract, and filtrate is subtracted
Pressure distillation, obtains the second extract, merges the first extract and the second extract, and adds lauryl sodium sulfate, obtains quality dense
Spend the liquid liquid phase for 10%.
S4, mass concentration for 15% stearic acid is configured to the aqueous solution.
S5, by step the S4 aqueous solution and deionized water in mass ratio be 0.3:1 ratio is weighed, and is heated to 70 DEG C, is obtained
Mixed liquor；
S6, in mass ratio be 1.5:The mixed liquor that 2 ratio prepares step S5 is added in S3 liquid liquid phases, at a high speed
20000 revs/min of lower uniform stirrings, obtain emulsion.
S7, emulsion is incubated 20min, regulation pH value obtains finished product to 6.5 when being cooled to 40 DEG C.
Application of the present invention also in plant pathogenic fungi and whitening sun protection.
Embodiment 3
A kind of preparation method of emulsion containing hill gooseberry, comprises the following steps：
S1,400g myrtle fruits and 100g Portugals peach fruit is clean, dries pulverizing.
S2, with volume fraction for 70% methanol solution at 50 DEG C of shaking bath mechanical shaking extraction 1.5h.Evaporation solvent is obtained
Filter residue, then filter residue is dissolved with chloroform, filtering obtains the first filter residue and filtrate.
S3, step S2 the first filter residue methanol dissolved, vacuum distillation solvent obtains the first extract, and filtrate is subtracted
Pressure distillation, obtains the second extract, merges the first extract and the second extract, and adds lauryl sodium sulfate, obtains quality dense
Spend the liquid liquid phase for 5%.
S4, by mass concentration it is that 5% lauryl sodium sulfate and stearic acid are configured to the aqueous solution.
S5, by step the S4 aqueous solution and deionized water in mass ratio be 0.2:1 ratio is weighed, and is heated to 65 DEG C, is obtained
Mixed liquor.
S6, in mass ratio be 0.8:The mixed liquor that 1.2 ratio prepares step S5 is added in S3 liquid liquid phases, at a high speed
20000 revs/min of lower uniform stirrings, obtain emulsion.
S7, emulsion is incubated 17min, regulation pH value obtains finished product to 5.5 when being cooled to 35 DEG C.
Application of the present invention also in plant pathogenic fungi and whitening sun protection.
Embodiment 4
A kind of preparation method of emulsion containing hill gooseberry, comprises the following steps：
S1,300g myrtle fruits and 100g Portugals peach fruit is clean, dries pulverizing.
S2, with volume fraction for 80% methanol solution at 60 DEG C of shaking bath mechanical shaking extraction 2h.Evaporation solvent must be filtered
Slag, then filter residue is dissolved with chloroform, filtering obtains the first filter residue and filtrate.
S3, step S2 the first filter residue methanol dissolved, vacuum distillation solvent obtains the first extract, and filtrate is subtracted
Pressure distillation, obtains the second extract, merges the first extract and the second extract, and adds lauryl sodium sulfate, obtains quality dense
Spend the liquid liquid phase for 8%.
S4, by mass concentration for 8% lauryl sodium sulfate and stearic acid be configured to the aqueous solution.
S5, by step the S4 aqueous solution and deionized water in mass ratio be 0.2:1 ratio is weighed, and is heated to 70 DEG C, is obtained
Mixed liquor.
S6, in mass ratio be 0.8:The mixed liquor that 1 ratio prepares step S5 is added in S3 liquid liquid phases, at a high speed
20000 revs/min of lower uniform stirrings, obtain emulsion.
S7, emulsion is incubated 20min, regulation pH value obtains finished product to 5.5 when being cooled to 30 DEG C.
Application of the present invention also in plant pathogenic fungi and whitening sun protection.
In order to verify beneficial effects of the present invention, the present invention has done following experiment：
Comparative example 1
The difference of the comparative example and embodiment 4 is：
S2, with volume fraction for 80% methanol solution mechanical shaking extraction 2h, filtering at 60 DEG C of shaking bath, obtain filter residue and
Filtrate.
S3, step S2 filter residue dissolved with methanol, vacuum distillation solvent, obtain the first extract, filtrate carries out decompression steaming
Evaporate, obtain the second extract, merge the first extract and the second extract, obtain the liquid liquid phase that mass concentration is 8%.
Comparative example 2
The difference of the comparative example and embodiment 4 is：
S2, with volume fraction for 80% ethanol solution mechanical shaking extraction 2h, filtering at 60 DEG C of shaking bath, obtain filter residue and
Filtrate.
S3, step S2 filter residue dissolved with ethanol, vacuum distillation solvent, obtain the first extract, filtrate carries out decompression steaming
Evaporate, obtain the second extract, merge the first extract and the second extract, obtain the liquid liquid phase that mass concentration is 8%.
Comparative example 3
The difference of the comparative example and embodiment 4 is：
S2, with volume fraction for 80% ethanol solution at 60 DEG C of shaking bath mechanical shaking extraction 2h filter, obtain filter residue and
Filtrate.
S3, step S2 filter residue dissolved with ethanol, vacuum distillation solvent, obtain the first extract, filtrate carries out decompression steaming
Evaporate, obtain the second extract, merge the first extract and the second extract, obtain the liquid liquid phase that mass concentration is 8%.
S4, mass concentration for 8% polyglyceryl fatty acid ester is configured to the aqueous solution.
Comparative example 4
The difference of the comparative example and embodiment 4 is：
S6, in mass ratio be 2:The mixed liquor that 3 ratio prepares step S5 is added in S3 liquid liquid phases, high speed 20000
Rev/min lower uniform stirring, obtains emulsion.
1 materials and methods
1.1 for examination strain
Predominantly the husky skin of the black shin of wild cabbage blackspot, tobacco, capsicum anthrax, paddy rice oriental sesame leaf, cumquat, sugarcane pineapple, corn are big
The disease funguses such as spot, tea wheel spot and tribute mandarin orange chain lattice.
1.2 inhibitory activity are tested
Cultured pathogenic bacteria are diluted with nutrient solution, dilution factor is 1:500-1:1000, bacterium will be contained after dilution
Nutrient solution is quantitatively added in each hole of 96 orifice plates respectively, and the emulsion of embodiment 4 is configured into 1mg/mL, takes quantitatively add to respectively
In each hole.28 DEG C of incubated 48h in constant incubator are placed in after adding, each hole is then surveyed under 630nm with ELIASA and inhales
Luminosity, calculates growth inhibition ratio.
2 results and analysis
2.1 bacteriostatic activities of emulsion containing hill gooseberry are shown in Table 1：
Emulsion containing myrtle fruit and the preparation of the extract of Portugal peach fruit is to inhibition of germination and mycelial growth inhibition rate
Mostly more than 80%, show that the emulsion shows preferable inhibition to pathogen spore germination and mycelial growth.
2.2 disease funguses (wild cabbage blackspot) inhibitory activity is tested：
The growth inhibition ratio of the disease fungus of embodiment 1~4 and comparative example 1~4 is calculated, 2 are shown in Table：
| Group | Inhibition of germination/% | Mycelial growth inhibition rate/% |
| Embodiment 1 | 83.6 | 85.4 |
| Embodiment 2 | 85.4 | 89.7 |
| Embodiment 3 | 86.3 | 90.8 |
| Embodiment 4 | 89.3 | 92.4 |
| Comparative example 1 | 70.2 | 72.3 |
| Comparative example 2 | 71.6 | 75.3 |
| Comparative example 3 | 65.4 | 68.7 |
| Comparative example 4 | 72.3 | 73.5 |
As seen from the above table, emulsion of the present invention has the effect for suppressing fungi well, by the preparation process of the present invention, makes
The a variety of extracts for obtaining myrtle fruit and Portugal peach fruit are fully extracted, and in extract prepared by different solvents, content can not yet
Together, it is primarily due to match by its operating procedure, the polarity of solvent and its active material, passes through pre-emulsification and the work of emulsification
With making a variety of extracts be difficult to be oxidized so that the emulsion being prepared into has the effect for suppressing fungi.
The present invention has also done following experiment：
First, the preparation method according to embodiment 4, using opposed polarity solvent extractable matter to the growth inhibition situation of fungi such as
Shown in table 3 below：
| Plant pathogenic fungi | Methanol | Ethanol | Chloroform | Ether | Ethyl acetate | Methanol+chloroform |
| Wild cabbage blackspot | 80.1 | 75.4 | 70.4 | 60.2 | 62.3 | 85.9 |
| The black shin of tobacco | 76.7 | 78.1 | 73.1 | 72.1 | 70.3 | 86.7 |
| Capsicum anthrax | 76.5 | 72.4 | 73.6 | 68.0 | 70.5 | 86.4 |
| Paddy rice oriental sesame leaf | 82.1 | 76.4 | 75.1 | 71.3 | 68.5 | 90.1 |
| Cumquat sand skin | 80.7 | 78.2 | 70.2 | 72.4 | 70.6 | 88.3 |
| Sugarcane pineapple | 82.1 | 76.3 | 73.8 | 68.6 | 73.5 | 92.8 |
| The big spot of corn | 80.5 | 70.5 | 71.5 | 70.9 | 62.9 | 90.8 |
| Tea wheel spot | 73.7 | 70.6 | 74.1 | 65.3 | 65.8 | 93.4 |
| Tribute mandarin orange chain lattice | 74.9 | 75.1 | 76.5 | 62.7 | 72.8 | 94.2 |
As seen from the above table, the emulsion prepared in methanol and chloroform lytic agent has to plant pathogenic fungi preferably to be suppressed to make
With inhibiting rate is up to more than 85%, and emulsion inhibition prepared by other solvent extractable matters is poor, shows to suppress the work of mycelial growth
Property material may only concentrate be dissolved in methanol and chloroform polar component.
2nd, the anti-ultraviolet property of emulsion
According to the size of energy, ultraviolet is divided into 3 regions, is UV-A regions, and wavelength is 320~400nm, solar energy
It is minimum, it is general only to make skin tanning；Two be the higher UVB regions of energy, 290~320nm of wavelength, and it can tanned sunburn skin
Skin；Three be the UVC regions that wavelength is 200~290nm, solar energy highest, so the lethality to skin is also very big.
Blank example 1 is set：It is blank assay 1 by the experimental group for not smearing hill gooseberry's emulsion.
Embodiment 1~4, comparative example 3 are applied on slide, after timebase, scanning sample is in UVA, UVB, UVC
UV absorption average value.Testing result is shown in Table 4：
It has been shown that, emulsion is good to ultraviolet radiation absorption effect made from embodiment 1~4 possess preferably anti-by the result of table 4
Effect is shone, comparative example 3 is extracted only with single ethanol, and pre-emulsification and the effect emulsified is carried out to extract,
By relatively of the invention preferable to the assimilation effect of ultraviolet compared with embodiment 3.
3rd, emulsion is determined to tyrosinase inhibitory activity
Tyrosinase is a kind of oxidoreducing enzyme, and tyrosinase oxidation can promote TYR to generate melanin, in junket
During propylhomoserin formation melanin, tyrosinase, which can promote TYR to produce L-3,4 dihydroxyphenylalanine and further act on, makes L-3,4 dihydroxyphenylalanine shape
Into bar quinone, DOPA quinone ultimately generates melanin.The calculation formula of inhibiting rate：[1- (T2-T1)/(C2-C1)] * 100%
C1 is the absorbance of cushioning liquid and tyrosinase extract solution, and C2 represents the absorbance of the melanin of generation,
The absorbance that C2 is surveyed by reference of C1 is C2-C1, and it is black that expression is generated after the absorbance of tyrosinase extract solution is deducted
The absorbance of pigment.T1 is the absorbance for adding sample, tyrosinase extract solution and cushioning liquid, and T2 is pressed down by sample
The absorbance of the melanin generated after system, the same absorbance that T2 is surveyed by reference of T1 obtains T2-T1, represents deducting
Under the influence of sample and tyrosinase extract solution absorbance, the absorbance of the melanin after the suppression of sample.Embodiment 1
Inhibiting rate knot of the sample solution obtained to embodiment 4 and comparative example 1 to comparative example 4 in tyrosinase inhibitory activity measure
Fruit is as shown in table 5：
The emulsion containing hill gooseberry and Portugal's peach extract that embodiment 1 to embodiment 4 is obtained is strong to tyrosinase rejection ability,
It can be used for skin-whitening.
In summary, using provided by the present invention the invention discloses a kind of preparation method of emulsion containing hill gooseberry, its
It is easily operated in preparation process.Myrtle fruit and Portugal peach fruit are extracted by the combined use of methanol and chloroform, and passed through
Dissolving is extracted repeatedly, fully extracts phenols, flavonoids and Anthraquinones various active chemical composition；Add dodecyl sulphate
Sodium obtains liquid liquid phase into extract, and pre-emulsification extract makes extract not oxidizable；Lauryl sodium sulfate or tristearin
Acid or combination are configured to the preferable mixed liquor of stability so that mixed liquor is further emulsified to primary emulsifying extract
Effect, the finished product of preparation fully has protective effect to phenolic compound, ketone etc., makes its not oxidation by air, for planting
In the preventing and treating and whitening sun protection cosmetics of thing disease fungus, the effect with good curative effect, with stability.This process simplify
Intermediate steps, make preparation process more efficiently and environmentally friendly, and cost is also accordingly reduced.Avoid the use of other chemical substance agricultural chemicals
Pollution on the environment, crops are high to its bioavailability, and effect is notable；Extensively, environmental protection is degradable for raw material sources.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God is with principle, and any modification, equivalent substitution and improvements done etc. should be included within the scope of protection of the invention.
Claims (9)
1. the preparation method of a kind of emulsion containing hill gooseberry, it is characterised in that comprise the following steps：
S1, myrtle fruit and Portugal peach fruit is clean, dries pulverizing；
S2, extracted with methanol solution, evaporation solvent obtains filter residue, then dissolves filter residue with chloroform, and filtering obtains the first filter residue and filter
Liquid；
S3, step S2 the first filter residue methanol dissolved, vacuum distillation solvent, obtain the first extract, filtrate carries out decompression steaming
Evaporate, obtain the second extract, merge the first extract and the second extract, and add lauryl sodium sulfate, obtain liquid liquid phase；
S4, at least one of lauryl sodium sulfate or stearic acid be configured to the aqueous solution；
S5, by step the S4 aqueous solution and deionized water in mass ratio be 0.1~0.3:1 ratio is weighed, and is heated to 60~70
DEG C, obtain mixed liquor；
S6, in mass ratio be 0.5~1.5:The mixed liquor that 1~2 ratio prepares step S5 is added in S3 liquid liquid phases, at a high speed
Stirring, obtains emulsion；
S7, by emulsion be incubated 15~20min, cooling regulation pH value to 4.5~6.5, obtain finished product.
2. a kind of preparation method of emulsion containing hill gooseberry according to claim 1, it is characterised in that peach in the step S1
The mass ratio of golden ma fruit and Portugal peach fruit is 3:1~5:1.
3. the preparation method of a kind of emulsion containing hill gooseberry according to claim 1, it is characterised in that in the step S2,
With volume fraction for 60~80% methanol solution at 45 DEG C~60 DEG C of shaking bath 1~2h of mechanical shaking extraction.
4. a kind of preparation method of emulsion containing hill gooseberry according to claim 1, it is characterised in that liquid in the step S3
The mass concentration of body fluid phase is 0.1~10%.
5. the preparation method of a kind of emulsion containing hill gooseberry according to claim 1, it is characterised in that ten in the step S4
Sodium dialkyl sulfate or stearic mass concentration are 0.1~15%.
6. the preparation method of a kind of emulsion containing hill gooseberry according to claim 1, it is characterised in that pressed in the step S6
Mass ratio is 0.5~0.8:The mixed liquor that 1~1.2 ratio prepares step S5 is added in S3 liquid liquid phases.
7. a kind of preparation method of emulsion containing hill gooseberry according to claim 1, it is characterised in that in the step S6
20000 revs/min of lower uniform stirrings at a high speed.
8. the preparation method of a kind of emulsion containing hill gooseberry according to claim 1, it is characterised in that dropped in the step S7
PH value is adjusted when temperature is to 30~40 DEG C.
9. hill gooseberry's emulsion according to any one of claim 1~8 is applied in plant pathogenic fungi and whitening sun protection.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108403545A (en) * | 2018-03-28 | 2018-08-17 | 广西秀美壮乡能源环保有限公司 | A kind of selenium-rich hill gooseberry freckle removing and whitening liquid and preparation method thereof |
| CN115120520A (en) * | 2022-05-06 | 2022-09-30 | 彭氏(惠州)实业发展有限公司 | Cosmetic composition comprising myrtle ketone and cosmetic use thereof |
| CN115710245A (en) * | 2022-10-25 | 2023-02-24 | 中国科学院华南植物园 | Myrtle ketone industrial extraction method and myrtle ketone microcapsule preparation method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102006856A (en) * | 2008-04-16 | 2011-04-06 | 皮埃尔·法布尔皮肤化妆品公司 | Use of myrtle extract as depigmenting agent |
| CN104582827A (en) * | 2012-09-28 | 2015-04-29 | 富士胶片株式会社 | Carotenoid-containing oil-in-water emulsion composition |
| CN104661739A (en) * | 2012-09-28 | 2015-05-27 | 富士胶片株式会社 | Lycopene-containing oil-in-water emulsion composition and production method therefor |
| CN105434256A (en) * | 2015-12-18 | 2016-03-30 | 山东福瑞达生物工程有限公司 | Natural emulsion, and preparation method and applications thereof |
-
2017
- 2017-02-06 CN CN201710065701.7A patent/CN107233241A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102006856A (en) * | 2008-04-16 | 2011-04-06 | 皮埃尔·法布尔皮肤化妆品公司 | Use of myrtle extract as depigmenting agent |
| CN104582827A (en) * | 2012-09-28 | 2015-04-29 | 富士胶片株式会社 | Carotenoid-containing oil-in-water emulsion composition |
| CN104661739A (en) * | 2012-09-28 | 2015-05-27 | 富士胶片株式会社 | Lycopene-containing oil-in-water emulsion composition and production method therefor |
| CN105434256A (en) * | 2015-12-18 | 2016-03-30 | 山东福瑞达生物工程有限公司 | Natural emulsion, and preparation method and applications thereof |
Non-Patent Citations (9)
| Title |
|---|
| 刘伟等: "桃金娘化学成分及生物活性的研究进展", 《中国畜牧兽医》 * |
| 刘国坤等: "几种植物提取物对4 种植物病原真菌的抑制作用", 《福建农林大学学报（自然科学版）》 * |
| 周学明等: "桃金娘叶的化学成分研究", 《中草药》 * |
| 张爱珍等: "《临床营养护理》", 31 August 2013, 浙江大学出版社 * |
| 杨为海等: "桃金娘果实提取物抗氧化活性研究", 《热带作物学报》 * |
| 桥明福等: "蒲桃不同提取部位总黄酮含量比较研究", 《广州化工》 * |
| 王昌涛等: "《化妆品植物添加剂的开发与应用》", 30 April 2013, 中国轻工业出版社 * |
| 翟中和等: "《细胞生物学》", 31 May 2001, 高等教育出版社 * |
| 郑晓燕等: "桃金娘果实多酚抗氧化活性及乙酰胆碱酯酶抑制活性研究", 《中国农学通报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108403545A (en) * | 2018-03-28 | 2018-08-17 | 广西秀美壮乡能源环保有限公司 | A kind of selenium-rich hill gooseberry freckle removing and whitening liquid and preparation method thereof |
| CN115120520A (en) * | 2022-05-06 | 2022-09-30 | 彭氏(惠州)实业发展有限公司 | Cosmetic composition comprising myrtle ketone and cosmetic use thereof |
| CN115710245A (en) * | 2022-10-25 | 2023-02-24 | 中国科学院华南植物园 | Myrtle ketone industrial extraction method and myrtle ketone microcapsule preparation method |
| CN115710245B (en) * | 2022-10-25 | 2023-08-08 | 中国科学院华南植物园 | Industrial extraction method of myrtle ketone and preparation method of myrtle ketone microcapsule |
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