CN107197641A - A kind of efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method - Google Patents
A kind of efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method Download PDFInfo
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- 238000009331 sowing Methods 0.000 claims abstract description 42
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 22
- 230000001954 sterilising effect Effects 0.000 claims abstract description 21
- 238000005286 illumination Methods 0.000 claims abstract description 10
- 230000006378 damage Effects 0.000 claims abstract description 9
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- 239000010451 perlite Substances 0.000 claims description 16
- 235000019362 perlite Nutrition 0.000 claims description 16
- 239000010455 vermiculite Substances 0.000 claims description 16
- 235000019354 vermiculite Nutrition 0.000 claims description 16
- 229910052902 vermiculite Inorganic materials 0.000 claims description 16
- VEWUNRJQQRKSCB-UHFFFAOYSA-N 6-benzylpurine-2,6-diamine Chemical class C12=NC=NC2=NC(N)=NC1(N)CC1=CC=CC=C1 VEWUNRJQQRKSCB-UHFFFAOYSA-N 0.000 claims description 15
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention provides a kind of efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method, comprises the following steps：Select no disease and pests harm and atraumatic hybrid seed；Hybrid seed is carried out disinfection, sterile hybrid seed is obtained；Sowing media is sterilized, then aseptically, in the fine sand that the hybrid seed after sterilization is seeded in after sterilizing or the fine sand added with 6 benzyl aminoadenines；After planting, hybrid seed is cultivated to cotyledon in the case where temperature is 23~25 DEG C of full dark condition exposes sowing media, then temperature be 23~25 DEG C, natural scattered light intensity of illumination be that to cultivate to the height of seedling of seedling under conditions of 2800~3200lx be 4~5cm, then transplant to the transplanting medium being placed in during air humidity is the closed environment that 80~90%, temperature is 23~25 DEG C.The present invention can improve the germination percentage of Camellia nitidissima hybrid seed, improve seeding and seedling raising efficiency, reduce hybridization germplasm loss.
Description
Technical field
The present invention relates to botanical seedling culturing technical field, more particularly to a kind of efficient Camellia nitidissima hybrid seed aseptic seeding is educated
Seedling-growing method.
Background technology
Camellia nitidissima (Camellia chrysantha (Hu) Tuyama) is unique karat gold yellow in Theaceae Camellia Plants
Colored rare ornamental plant, its golden yellow flower is unique, great gloss, therefore has " giant panda of plant kingdom ", " tea race queen "
Laudatory title.Camellia nitidissima distant hybridization breeding, percentage of fertile fruit as little as 1.5% adds cenospecies part embryo deformity or depauperation, because
This, Camellia nitidissima hybrid seed is of great rarity.And the emergence rate of Camellia nitidissima hybrid seed conventional seed planting nursery is extremely low, about 7.5%, gold
The hybridization germplasm loss of jasmine tea is serious.
The content of the invention
It is an object of the invention to overcome drawbacks described above to educate there is provided a kind of efficient Camellia nitidissima hybrid seed aseptic seeding
Seedling-growing method, the aseptic seeding growing seedlings method is by providing the sterile rearing condition of thermophilic, and addition plant growth regulator promotes
Hybridize the division of embryo (separate living tissue), improve the germination percentage of Camellia nitidissima hybrid seed, and sprout ratio, reach that raising sowing is educated
Seedling efficiency, reduces the effect of hybridization germplasm loss.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is：
The present invention provides a kind of efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method, comprises the following steps：
Step 1：Select no disease and pests harm and atraumatic hybrid seed；
Step 2：The hybrid seed of step 1 is carried out disinfection, sterile hybrid seed is obtained；
Step 3：Sowing media is sterilized, then aseptically, the hybrid seed sowing after step 2 is sterilized
In the sowing media after sterilizing, wherein, sowing media is fine sand or addition 6- benzyl aminoadenine solution (concentration 0.5mg
L-1) fine sand；
Step 4：After planting, the hybrid seed of sowing media is seeded in cultivate in the case where temperature is 23~25 DEG C of full dark condition
Expose sowing media to cotyledon, then in the bar that temperature is 23~25 DEG C, natural scattered light intensity of illumination is 2800~3200lx
It is 4~5cm to be cultivated under part to the height of seedling of seedling, and it is that 80~90%, temperature is 23~25 then to transplant to air humidity is placed in
DEG C closed environment in transplanting medium.
The beneficial effects of the present invention are：(1) present invention is seeded in temperature by the way that Camellia nitidissima hybrid seed is carried out disinfection
Degree, damp condition are suitable, addition concentration 0.5mgL-1Cultivated on the sterile fine sand of 6- benzyl aminoadenine solution, compared to existing
Camellia nitidissima hybrid seed seeding growing seedlings method (germination percentage 54.2%) for, the germination percentage of the application up to more than 79.2%,
Effectively increase the germination percentage (the seed number % that the seed number of germination × 100/ is sowed) of Camellia nitidissima hybrid seed；(2) by broadcasting
The rush cell division effect of 6- benzyl aminoadenines in matrix (fine sand) is planted, the further sprouting ratio for improving seed be (sprouting
The seed number % that sprout number × 100/ is sowed) so that sprout ratio and be up to 137.5%, effectively facilitate plumule and sprout many seedlings, improve
Seeding and growing seedling efficiency, reduces Camellia nitidissima hybridization germplasm loss；(3) seeding growing seedlings method of the invention, relatively existing routine
For sowing, the insemination and emergence time is greatly shortened about 30-35 days, shorten the time of nearly half, further increase nursery and broadcast
Plant efficiency.(4) for seeding growing seedlings method of the invention, relatively existing conventional seed planting, seedling-growing time neat and consistent, and manage just
It is prompt.
Embodiment
To describe the technology contents of the present invention in detail, feature, the objects and the effects being constructed, below in conjunction with embodiment
It is explained in detail.
The design of most critical of the present invention is：In warm, wet suitable Sterile culture room, hybrid seed is seeded in and soaked
On the sterile fine sand of 6- benzyl aminoadenines cultivate, with improve hybrid seed germination percentage and sprout ratio, realize high efficiency seedling cultivating,
Easy to operate, management is convenient.
The present invention provides a kind of efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method, comprises the following steps：
Step 1：Select full kind of grain, no disease and pests harm and atraumatic hybrid seed；
Step 2：The hybrid seed of step 1 is carried out disinfection, sterile hybrid seed is obtained；
Step 3：Sowing media is sterilized, then aseptically, the hybrid seed sowing after step 2 is sterilized
In the sowing media after sterilizing, wherein, sowing media is added with concentration 0.5mgL-16- benzyls aminoadenine (6-BA)
Fine sand；
Step 4：After planting, the hybrid seed of sowing media is seeded in cultivate in the case where temperature is 23~25 DEG C of full dark condition
Expose sowing media to cotyledon, then in the bar that temperature is 23~25 DEG C, natural scattered light intensity of illumination is 2800~3200lx
It is 4~5cm to be cultivated under part to the height of seedling of seedling, and it is that 80~90%, temperature is 23~25 then to transplant to air humidity is placed in
DEG C closed environment in transplanting medium.
The operation principle of the present invention：Hybrid seed after sterilization is seeded in the fine sand by sterilizing under gnotobasis,
Can effectively reduce the microbial destruction such as bacterium seed vigor (causing seed nutritional component damages), and the water content of fine sand and
The sprouting of the suitable seed of gas permeability, 6- benzyl aminoadenines are added in fine sand, embryo (separate living tissue) can be effectively facilitated in addition
Division, reach many young plants of seed direct germination, at the same control seed sprout temperature be 23~25 DEG C, and then cause it is miscellaneous
Hand over the germination percentage of seed and sprout ratio height, shorten the seeding and seedling raising time (aseptic seeding about 20d, you can see most seed kind skins
Cracking, 10~13d is only needed from kind of skin cracking to prominent break in the seed coat of radicle, from cotyledon expose matrix, epicotyl elongation, plumule sprout and
Expansion true leaf only needs 10~15d, and true leaf is larger, wide up to 2.0cm；And existing conventional seed planting about 30~35d kinds skin ftractures, extremely
Germination of breaking ground about needs 75d), the environmental condition transplanted in addition by control so that the kind shoot survival percent of transplanting is high.
It was found from foregoing description, the beneficial effects of the present invention are：(1) present invention is by the way that Camellia nitidissima hybrid seed is carried out
Sterilization, and be seeded in be placed on the fine sand in the suitable gnotobasis of temperature, damp condition cultivate, compared to existing Camellia nitidissima
For the seeding growing seedlings method (germination percentage 54.2%) of hybrid seed, the germination percentage of the application is effectively improved up to more than 79.2%
The germination percentage of Camellia nitidissima hybrid seed；(2) by adding 6- benzyl aminoadenines on the sowing media of seed (fine sand), enter
One step improves the sprouting ratio of seed so that sprouts ratio and is up to 137.5%, effectively facilitates plumule and sprouts many seedlings, improves and educate
Seedling sows efficiency, reduces Camellia nitidissima hybridization germplasm loss；(3) seeding growing seedlings method of the invention, relatively existing conventional seed planting
For, the seeding and seedling raising time is greatly shortened, shortens the time of nearly half, further increases seeding and growing seedling efficiency.
Further, the cenospecies seed be using concave veins Camellia nitidissima be maternal, ' row are fragrant ' camellia is hybridized as male parent
Obtained hybrid seed or by maternal, var. longistyla of yellow cup Camellia nitidissima be male parent hybridize obtained hybrid seed or
Person's var. longistyla is that to embrace stem tea be the obtained hybrid seed that male parent is hybridized for maternal, Vietnam.
Seen from the above description, hybrid seed is selected from above-mentioned 3 kinds of hybrid seeds, above-mentioned 3 kinds of Camellia nitidissimas cross combination fruit setting
The relatively other Camellia nitidissima cross combination percentages of fertile fruit of rate are high, seed material is more, real as Camellia nitidissima hybrid seed aseptic seeding
Test more representative.
Further, the concrete operations of the step 2 are：By the wine that the hybrid seed volumetric concentration of step 1 is 75%
Progress greatly row 50~70s of surface sterilization, then with aseptic water washing 2~4 times, then with the hypochlorous acid that mass concentration is 2.2~2.6%
Sodium solution carries out disinfection 4~5min, finally with aseptic water washing more than 5 times, wherein, the time of each aseptic water washing is
2min。
Seen from the above description, Camellia nitidissima hybrid seed is carried out disinfection using aforesaid way, sterilized more abundant, it is to avoid miscellaneous
The harmful substances such as bacterium or virus that seed is carried in itself are handed over to influence the sprouting in hybrid seed later stage.
Further, the concrete operations of step 3 are：By sowing media be placed in temperature be 121 DEG C, pressure be 1.1kg/cm2
Under conditions of sterilize 25~35min, then aseptically, the hybrid seed after step 2 is sterilized be seeded in sterilizing after
In sowing media, wherein, sowing media is for fine sand or added with (concentration 0.5mgL-1) 6- benzyl aminoadenines fine sand.
Seen from the above description, the sterilizing that sowing media is carried out under these conditions make it that sterilizing is more complete, it is ensured that accomplish
Aseptic seeding.
Further, the transplanting medium is simple yellow soil, the yellow soil added with vermiculite, the Huang added with perlite
One kind in cubsoil and yellow soil added with coconut palm chaff, wherein the quality of the yellow soil and vermiculite in the yellow soil added with vermiculite
Than for 3: 1, the mass ratio of yellow soil and perlite in the yellow soil added with perlite is 3: 1, the gold zone added with coconut palm chaff
The mass ratio of yellow soil and coconut palm chaff in soil is 3: 1.
Further, in step 4, after the height of seedling of culture to seedling is 4~5cm, plant before transplantation of seedlings, seedling is carried out
Hardening 10~15 days.
Seen from the above description, in kind of a transplantation of seedlings advance row hardening, the adaptability of seedling late growing stage can be improved.
Further, the enclosed environment of step 4 is to be formed by plastic sheeting and sunshade rate for 75% Shading net covering.
Further, the preparation method of the fine sand added with 6- benzyl aminoadenines is that fine sand clear water is eluriated 2 times
More than, impurity is removed, is filtered dry；The fine sand being filtered dry is soaked in mass concentration for 0.5mgL-1The solution of 6- benzyl aminoadenines
Middle 10-30min, is finally picked up fine sand with filter screen.
Embodiment 1
A kind of efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method, comprises the following steps：
Step 1：Select full kind of grain, no disease and pests harm and atraumatic hybrid seed (concave veins Camellia nitidissima ♀ and ' strong perfume (or spice) ' tea
The seed of flower ♂ cenospecies)；
Step 2：The hybrid seed of step 1 is subjected to surface sterilization 50s with volumetric concentration for 75% alcohol, then with nothing
Bacterium water is rinsed 2 times, then is carried out disinfection 4min for 2.2% liquor natrii hypochloritis with mass concentration, finally with aseptic water washing 5 times
More than, sterile hybrid seed is obtained, wherein, the time of each aseptic water washing is 2min；
Step 3：The fine sand that cleaning is drained is divided in blake bottle, per bottled fine sand thickness about 2cm, cultivates bottle closure,
Be subsequently placed in temperature be 121 DEG C, pressure be 1.1kg/cm2Under conditions of sterilize 25min, then aseptically, by step 2
Hybrid seed after sterilization is seeded in the fine sand (pH value is 5.8) after sterilizing, and seed does not expose fine sand and is defined, and then seals；
Step 4：After planting, the hybrid seed of sowing media is seeded in sterile culture of the temperature for 23 DEG C of full dark condition
Room, which is cultivated to cotyledon, exposes sowing media, is then 23 DEG C, under conditions of natural scattered light intensity of illumination is 2800lx in temperature
It is 4~5cm to cultivate to the height of seedling of seedling, and the bottle cap hardening for opening blake bottle (broadcasts seedling without strain for raising and adapt to have collarium for 10 days
The ability in border, will broadcast seedling without strain and goes under the condition of culture that temperature is 20 DEG C, natural scattered light intensity of illumination is 3500lx and train
Support 10 days), then transplant into transplanting medium, use plastic sheeting and sunshade rate to be formed for 75% Shading net covering after transplanting
Closed management, the air humidity in enclosed environment is that 80%, temperature is 23 DEG C.
Wherein, 4 kinds of transplanting mediums：Yellow soil, the yellow soil+vermiculite (mass ratio 3 of yellow soil and vermiculite：1), yellow soil+
Perlite (the mass ratio 3 of yellow soil and perlite：1), yellow soil+coconut palm chaff (yellow soil and mass ratio of coconut palm chaff 3：1), Mei Zhongji
100 plants of rooted seedlings of replanting, transplant 50d investigation survival rates, transplanting survival rate is up to 100%.
The germination percentage (the seed number % that the seed number of germination × 100/ is sowed) and the sprouting ratio (bud of sprouting of the present embodiment
The seed number % that seedling number × 100/ is sowed) it the results are shown in Table 1.
Embodiment 2
A kind of efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method, comprises the following steps：
Step 1：Select full kind of grain, no disease and pests harm and atraumatic hybrid seed (yellow cup Camellia nitidissima ♀ and anti-city golden flower
The seed of tea ♂ cenospecies)；
Step 2：The hybrid seed of step 1 is subjected to surface sterilization 70s with volumetric concentration for 75% alcohol, then with nothing
Bacterium water is rinsed 4 times, then is carried out disinfection 5min for 2.6% liquor natrii hypochloritis with mass concentration, finally with aseptic water washing 5 times
More than, sterile hybrid seed is obtained, wherein, the time of each aseptic water washing is 2min；
Step 3：The fine sand that cleaning is drained is divided in blake bottle, per bottled fine sand thickness about 2cm, cultivates bottle closure,
Be subsequently placed in temperature be 121 DEG C, pressure be 1.1kg/cm2Under conditions of sterilize 35min, then aseptically, by step 2
Hybrid seed after sterilization is seeded in the fine sand (pH value is 5.8) after sterilizing, and seed does not expose fine sand and is defined, and then seals；
Step 4：After planting, the hybrid seed of sowing media is seeded in sterile culture of the temperature for 25 DEG C of full dark condition
Room, which is cultivated to cotyledon, exposes sowing media, is then 25 DEG C, under conditions of natural scattered light intensity of illumination is 3200lx in temperature
It is 4~5cm to cultivate to the height of seedling of seedling, open blake bottle bottle cap hardening 15 days (will be broadcast without strain seedling go to temperature for 30 DEG C,
Natural scattered light intensity of illumination is culture under 5000lx condition of culture 15 days), then transplant into transplanting medium, adopted after transplanting
With the closed management of plastic sheeting and sunshade rate for 75% Shading net covering, the air humidity in enclosed environment is
90%th, temperature is 25 DEG C.
Wherein, 4 kinds of transplanting mediums：Simple yellow soil, the yellow soil+vermiculite (mass ratio 3 of yellow soil and vermiculite：1), gold zone
Soil+perlite (the mass ratio 3 of yellow soil and perlite：1), yellow soil+coconut palm chaff (yellow soil and mass ratio of coconut palm chaff 3：1), often
Kind of matrix transplants 100 plants of rooted seedlings, transplants 50d investigation survival rates, and transplanting survival rate is up to 100%.
The germination percentage (the seed number % that the seed number of germination × 100/ is sowed) and the sprouting ratio (bud of sprouting of the present embodiment
The seed number % that seedling number × 100/ is sowed) it the results are shown in Table 1.
Embodiment 3
A kind of efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method, comprises the following steps：
Step 1：Selecting full kind of grain, no disease and pests harm and atraumatic hybrid seed, (stem is embraced by var. longistyla ♀ and Vietnam
The seed of tea ♂ cenospecies)；
Step 2：The hybrid seed of step 1 is subjected to surface sterilization 60s with volumetric concentration for 75% alcohol, then with nothing
Bacterium water is rinsed 3 times, then is carried out disinfection 5min for 2.4% liquor natrii hypochloritis with mass concentration, finally with aseptic water washing 5 times
More than, sterile hybrid seed is obtained, wherein, the time of each aseptic water washing is 2min；
Step 3：The fine sand for cleaning drip is divided in blake bottle, per bottled fine sand thickness about 2cm, bottle closure is cultivated, so
After be placed in temperature be 121 DEG C, pressure be 1.1kg/cm2Under conditions of sterilize 30min, then aseptically, step 2 is disappeared
Hybrid seed after poison is seeded in the fine sand (pH value is 5.8) after sterilizing, and seed does not expose fine sand and is defined, and then seals；
Step 4：After planting, the hybrid seed of sowing media is seeded in sterile culture of the temperature for 24 DEG C of full dark condition
Room, which is cultivated to cotyledon, exposes sowing media, is then 24 DEG C, under conditions of natural scattered light intensity of illumination is 3000lx in temperature
It is 4~5cm to cultivate to the height of seedling of seedling, open blake bottle bottle cap hardening 12 days (will be broadcast without strain seedling go to temperature for 25 DEG C,
Natural scattered light intensity of illumination is culture under 4000lx condition of culture 12 days), then transplant into transplanting medium, adopted after transplanting
With the closed management of plastic sheeting and sunshade rate for 75% Shading net covering, the air humidity in enclosed environment is
85%th, temperature is 24 DEG C.
Wherein, 4 kinds of transplanting mediums：Yellow soil, the yellow soil+vermiculite (mass ratio 3 of yellow soil and vermiculite：1), yellow soil+
Perlite (the mass ratio 3 of yellow soil and perlite：1), yellow soil+coconut palm chaff (yellow soil and mass ratio of coconut palm chaff 3：1), Mei Zhongji
100 plants of rooted seedlings of replanting, transplant 50d investigation survival rates, transplanting survival rate is up to 100%.
The germination percentage (the seed number % that the seed number of germination × 100/ is sowed) and the sprouting ratio (bud of sprouting of the present embodiment
The seed number % that seedling number × 100/ is sowed) it the results are shown in Table 1.
Embodiment 4
The present embodiment be the same as Example 1, difference is, it is 0.5mgL that mass concentration is added with fine sand-16- benzyls
Aminoadenine (6-BA), specific method is：Fine sand is eluriated for several times with clear water, is removed impurity, is filtered dry；By the fine sand being filtered dry leaching
Steep in concentration 0.5mgL-1In the solution of 6- benzyl aminoadenines after 10min, fine sand is picked up with filter screen, blake bottle is divided in
In, per bottled fine sand thickness about 2cm, cultivate bottle closure.
4 kinds of transplanting mediums：Yellow soil, the yellow soil+vermiculite (mass ratio 3 of yellow soil and vermiculite：1), yellow soil+perlite
(the mass ratio 3 of yellow soil and perlite：1), yellow soil+coconut palm chaff (yellow soil and mass ratio of coconut palm chaff 3：1), every kind of matrix is transplanted
100 plants of rooted seedlings, transplant 50d investigation survival rates, transplanting survival rate is up to 100%.
The germination percentage (the seed number % that the seed number of germination × 100/ is sowed) and the sprouting ratio (bud of sprouting of the present embodiment
The seed number % that seedling number × 100/ is sowed) it the results are shown in Table 1.
Embodiment 5
The present embodiment be the same as Example 2, difference is, it is 0.5mgL that mass concentration is added with fine sand-16- benzyls
Aminoadenine (6-BA), specific method is：Fine sand is eluriated for several times with clear water, is removed impurity, is filtered dry；By the fine sand being filtered dry leaching
Steep in concentration 0.5mgL-1In the solution of 6- benzyl aminoadenines after 30min, fine sand is picked up with filter screen, blake bottle is divided in
In, per bottled fine sand thickness about 2cm, cultivate bottle closure.
Wherein, 4 kinds of transplanting mediums：Yellow soil, the yellow soil+vermiculite (mass ratio 3 of yellow soil and vermiculite：1), yellow soil+
Perlite (the mass ratio 3 of yellow soil and perlite：1), yellow soil+coconut palm chaff (yellow soil and mass ratio of coconut palm chaff 3：1), Mei Zhongji
100 plants of rooted seedlings of replanting, transplant 50d investigation survival rates, transplanting survival rate is up to 100%.
The germination percentage (the seed number % that the seed number of germination × 100/ is sowed) and the sprouting ratio (bud of sprouting of the present embodiment
The seed number % that seedling number × 100/ is sowed) it the results are shown in Table 1.
Embodiment 6
The present embodiment be the same as Example 3, difference is, it is 0.5mgL that mass concentration is added with fine sand-16- benzyls
Aminoadenine (6-BA), specific method is：Fine sand is eluriated for several times with clear water, is removed impurity, is filtered dry；By the fine sand being filtered dry leaching
Steep in concentration 0.5mgL-1In the solution of 6- benzyl aminoadenines after 30min, fine sand is picked up with filter screen, blake bottle is divided in
In, per bottled fine sand thickness about 2cm, cultivate bottle closure.
Wherein, 4 kinds of transplanting mediums：Yellow soil, the yellow soil+vermiculite (mass ratio 3 of yellow soil and vermiculite：1), yellow soil+
Perlite (the mass ratio 3 of yellow soil and perlite：1), yellow soil+coconut palm chaff (yellow soil and mass ratio of coconut palm chaff 3：1), Mei Zhongji
100 plants of rooted seedlings of replanting, transplant 50d investigation survival rates, transplanting survival rate is up to 100%.
The germination percentage (the seed number % that the seed number of germination × 100/ is sowed) and the sprouting ratio (bud of sprouting of the present embodiment
The seed number % that seedling number × 100/ is sowed) it the results are shown in Table 1.
Comparative example 1
Seed：Concave veins Camellia nitidissima ♀ and the seed of ' strong perfume (or spice) ' camellia ♂ cenospecies, hybrid seed require that kind of grain is full, disease-free
Insect pest, without wound；
Seed type of seeding：In simple plastic greenhouse, temperature is 15~30 DEG C, and seed is non-sterile, and seed is directly sowed
In mass ratio, yellow soil：Peat soil：Coconut palm chaff=1: after 1: 1 mixed-matrix (non-sterilized), earthing 1cm, cover film moisturizing
Insulation.
Germination percentage (the seed number % that the seed number of germination × 100/ is sowed) and sprouting ratio (sprout number × 100/ of sprouting
The seed number % of sowing) it the results are shown in Table 1.
Comparative example 2
This comparative example is with comparative example 1, and difference is, seed is Huang cup Camellia nitidissima ♀ and var. longistyla ♂ cenospecies
Seed.
Comparative example 3
This comparative example is with comparative example 1, and difference is, seed is that var. longistyla ♀ embraces stem tea ♂ cenospecies with Vietnam
Seed.
Comparative example 4
This comparative example be the same as Example 1, difference is, sowing media is changed to agar by fine sand.
Comparative example 5
This comparative example be the same as Example 2, difference is, sowing media is changed to agar by fine sand.
Comparative example 6
This comparative example be the same as Example 3, difference is, sowing media is changed to agar by fine sand.
Comparative example 7
This comparative example is with comparative example 4, and difference is, sowing media be changed to by agar be added with mass concentration
0.5mg·L-16- benzyls aminoadenine (6-BA) agar.
Comparative example 8
This comparative example is with comparative example 5, and difference is, sowing media be changed to by agar be added with mass concentration
0.5mg·L-16- benzyls aminoadenine (6-BA) agar.
Comparative example 9
This comparative example is with comparative example 6, and difference is, sowing media be changed to by agar be added with mass concentration
0.5mg·L-16- benzyls aminoadenine (6-BA) agar.
The germination percentage of table 1 and sprouting ratio
From table 1 it follows that the germination percentage of 3 groups of hybridization inter-species and sprout ratio and there is no notable difference, it is (flat with control group
Equal germination percentage implements 1~6 and significantly improves germination 54.2%) to compare.
In summary, a kind of efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method that the present invention is provided, the present invention
It is placed in by the way that Camellia nitidissima hybrid seed carries out disinfection, and is seeded in thin in the suitable gnotobasis of temperature, damp condition
Cultivated on sand, for the seeding growing seedlings method (germination percentage 54.2%) of existing Camellia nitidissima hybrid seed, the hair of the application
Bud rate effectively increases the germination percentage of Camellia nitidissima hybrid seed up to more than 79.2%；Pass through the sowing media (fine sand) in seed
Upper addition 6- benzyl aminoadenines, further improve the sprouting ratio of seed so that sprout ratio and are up to 137.5%, effectively promote
Enter plumule and sprout many seedlings, improve seeding and growing seedling efficiency, reduce Camellia nitidissima hybridization germplasm loss；The seeding and seedling raising side of the present invention
For method, relatively existing conventional seed planting, the seeding and seedling raising time is greatly shortened, shortens the time of nearly half, further improves
Seeding and growing seedling efficiency.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair
Equivalent structure or equivalent flow conversion that bright description is made, or directly or indirectly it is used in other related technology necks
Domain, is included within the scope of the present invention.
Claims (8)
1. a kind of efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method, it is characterised in that comprise the following steps：
Step 1：Select no disease and pests harm and atraumatic hybrid seed；
Step 2：The hybrid seed of step 1 is carried out disinfection, sterile hybrid seed is obtained；
Step 3：Sowing media is sterilized, then aseptically, the hybrid seed after step 2 is sterilized, which is seeded in, to go out
In sowing media after bacterium, wherein, sowing media is fine sand or the fine sand added with 6- benzyl aminoadenines；
Step 4：After planting, the hybrid seed of sowing media is seeded in cultivate to son in the case where temperature is 23~25 DEG C of full dark condition
Leaf exposes sowing media, is then 23~25 DEG C, under conditions of natural scattered light intensity of illumination is 2800~3200lx in temperature
It is 4~5cm to cultivate to the height of seedling of seedling, and it is that 80~90%, temperature is 23~25 DEG C then to transplant to air humidity is placed in
Transplanting medium in closed environment.
2. efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method according to claim 1, it is characterised in that described
It using concave veins Camellia nitidissima is that maternal, ' row are fragrant ' camellia is that male parent progress hybridizes obtained hybrid seed or with Huang that cenospecies seed, which is,
Cup Camellia nitidissima is maternal, var. longistyla is that male parent carries out hybridizing obtained hybrid seed or var. longistyla is maternal, got over
It is the obtained hybrid seed that male parent is hybridized that stem tea is embraced in south.
3. efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method according to claim 1, it is characterised in that described
The concrete operations of step 2 are：The hybrid seed of step 1 is subjected to 50~70s of surface sterilization with volumetric concentration for 75% alcohol,
Then with aseptic water washing 2~4 times, then carried out disinfection 4~5min for 2.2~2.6% liquor natrii hypochloritis with mass concentration,
Finally use aseptic water washing more than 5 times, wherein, the time of each aseptic water washing is 2min.
4. efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method according to claim 1, it is characterised in that step
3 concrete operations are：By sowing media be placed in temperature be 121 DEG C, pressure be 1.1kg/cm2Under conditions of sterilize 25~35min,
Then aseptically, the hybrid seed after step 2 is sterilized is seeded in the sowing media after sterilizing, wherein, sow base
Matter is fine sand or the fine sand added with 6- benzyl aminoadenines.
5. efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method according to claim 1, it is characterised in that described
Transplanting medium is simple yellow soil, the yellow soil added with vermiculite, the yellow soil added with perlite and the Huang added with coconut palm chaff
One kind in cubsoil.
6. efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method according to claim 1, it is characterised in that step
In 4, after the height of seedling of culture to seedling is 4~5cm, plant before transplantation of seedlings, seedling is subjected to hardening 10~15 days.
7. efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method according to claim 1, it is characterised in that step
4 enclosed environment is to be formed by plastic sheeting and sunshade rate for 75% Shading net covering.
8. efficient Camellia nitidissima hybrid seed aseptic seeding growing seedlings method according to claim 1, it is characterised in that described
The preparation method of fine sand added with 6- benzyl aminoadenines is that fine sand clear water is eluriated more than 2 times, removes impurity, is filtered dry；Will
The fine sand being filtered dry is soaked in mass concentration for 0.5mgL-110-30min in the solution of 6- benzyl aminoadenines, finally uses filter screen
Fine sand is picked up.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4818699A (en) * | 1983-08-22 | 1989-04-04 | Dna Plant Technology Corporation | Generation of somaclonal non-mendelian variants |
| CN106489741A (en) * | 2016-12-01 | 2017-03-15 | 广西壮族自治区林业科学研究院 | A kind of preparation method of Camellia nitidissima aseptic explant |
| CN106954513A (en) * | 2017-03-28 | 2017-07-18 | 揭阳市菁华种养专业合作社 | A kind of breeding method of Camellia nitidissima seedling |
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2017
- 2017-07-19 CN CN201710589485.6A patent/CN107197641B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4818699A (en) * | 1983-08-22 | 1989-04-04 | Dna Plant Technology Corporation | Generation of somaclonal non-mendelian variants |
| CN106489741A (en) * | 2016-12-01 | 2017-03-15 | 广西壮族自治区林业科学研究院 | A kind of preparation method of Camellia nitidissima aseptic explant |
| CN106954513A (en) * | 2017-03-28 | 2017-07-18 | 揭阳市菁华种养专业合作社 | A kind of breeding method of Camellia nitidissima seedling |
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| 曹孜义等编著: "《实用植物组织培养技术教程》", 30 November 1996, 甘肃科学技术出版社 * |
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