CN107189080A - A kind of preparation method of the regenerated silk fibroin hydrogel with graphene oxide - Google Patents
A kind of preparation method of the regenerated silk fibroin hydrogel with graphene oxide Download PDFInfo
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- CN107189080A CN107189080A CN201710366264.2A CN201710366264A CN107189080A CN 107189080 A CN107189080 A CN 107189080A CN 201710366264 A CN201710366264 A CN 201710366264A CN 107189080 A CN107189080 A CN 107189080A
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- graphene oxide
- silk fibroin
- regenerated silk
- liquid
- hydrogel
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/02—Elements
- C08K3/04—Carbon
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
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- Dispersion Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
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Abstract
The invention discloses a kind of preparation method of the regenerated silk fibroin hydrogel with graphene oxide, comprise the following steps：The salt that concentration is 1.0 ~ 10.0 wt% is mixed with the formic acid that concentration is 10 ~ 98 wt%, A liquid is designated as；Boiled silk is put into A liquid, after boiled silk dissolving, namely regenerated silk fibroin protein solution；Regenerated silk fibroin solution and graphene oxide dispersion are pressed into (7.5 ~ 9.5)：The ratio mixing of (2.5 ~ 0.5), and be sufficiently stirred for 3 ~ 10 minutes, it is designated as B liquid；B liquid is put into climatic chamber, the temperature of climatic chamber is set as 30 ~ 50 DEG C, and humidity set is 60 ~ 70%RH, makes its natural gel, you can obtaining has graphene oxide and regenerated silk fibroin hydrogel.By the above-mentioned means, the present invention can realize the dissolving of fibroin nanometer fibril level, reduce destruction to fibroin molecular structure, hydrogel mechanical property improved by the addition of graphene oxide, be it is a kind of it is simple, effectively, the method for preparing strong mechanical performance hydrogel.
Description
Technical field
The present invention relates to hydrogel preparation field, more particularly to a kind of regenerated silk fibroin water with graphene oxide
The preparation method of gel.
Background technology
Silk fibroin hydrogel is a kind of material between solid and liquid, and nontoxic, non-stimulated, biocompatibility is good
Good, the advantages of retentiveness is good can be widely applied to food, skin care item, biomimetic material, separator, insoluble drug release material etc.
Field.But existing silk fibroin hydrogel can not realize the dissolving of plain nanometer fibril level, and fibroin molecular structure can be produced
Raw strong destruction, and its hydrogel mechanical property is bad.
The content of the invention
The present invention solves the technical problem of provide a kind of regenerated silk fibroin hydrogel with graphene oxide
Preparation method, the dissolving of fibroin nanometer fibril level can be realized, reduce destruction to fibroin molecular structure, by aoxidizing stone
The addition of black alkene improves hydrogel mechanical property, be it is a kind of it is simple, effectively, the method for preparing strong mechanical performance hydrogel.
In order to solve the above technical problems, one aspect of the present invention is：There is provided a kind of with graphene oxide
Regenerated silk fibroin hydrogel preparation method, comprise the following steps：
The salt that concentration is 1.0 ~ 10.0 wt% is mixed with the formic acid that concentration is 10 ~ 98 wt%, A liquid is designated as；
Boiled silk is put into A liquid, after boiled silk dissolving, namely regenerated silk fibroin protein solution；
Regenerated silk fibroin solution and graphene oxide dispersion are pressed into (7.5 ~ 9.5)：The ratio mixing of (2.5 ~ 0.5), and fill
Divide stirring 3 ~ 10 minutes, be designated as B liquid；
B liquid is put into climatic chamber, the temperature of climatic chamber is set as 30 ~ 50 DEG C, and humidity set is 60 ~ 70%RH, is made
Its natural gel, you can obtaining has graphene oxide and regenerated silk fibroin hydrogel.
It is preferred that, the concentration of the regenerated silk fibroin solution is 10 ~ 30wt%.
It is preferred that, the mass fraction of the graphene oxide dispersion is 10 ~ 11%.
It is preferred that, the salt is one kind in lithium bromide, calcium chloride, magnesium chloride, lithium rhodanate, sodium sulfocyanate or wherein appoints
Several mixture of meaning.
The beneficial effects of the invention are as follows：The present invention can realize the dissolving of fibroin nanometer fibril level, reduce to fibroin point
The destruction of minor structure, hydrogel mechanical property is improved by the addition of graphene oxide, is that one kind is simple, effective, is prepared high-tensile strength
The method for learning performance hydrogel.
Embodiment
Presently preferred embodiments of the present invention is described in detail below so that advantages and features of the invention can be easier to by
It will be appreciated by those skilled in the art that apparent clearly being defined so as to be made to protection scope of the present invention.
The embodiment of the present invention includes：
A kind of preparation method of the regenerated silk fibroin hydrogel with graphene oxide, comprises the following steps：
The salt that concentration is 6 wt% is mixed with the formic acid that concentration is 60 wt%, A liquid is designated as；
Boiled silk is put into A liquid, after boiled silk dissolving, namely regenerated silk fibroin protein solution；
Regenerated silk fibroin solution and graphene oxide dispersion are pressed 8：2 ratio mixing, and be sufficiently stirred for 9 minutes, it is designated as B
Liquid；
B liquid is put into climatic chamber, the temperature of climatic chamber is set as 37 DEG C, and humidity set is 65%RH, makes its nature
Gel, you can obtaining has graphene oxide and regenerated silk fibroin hydrogel.
The concentration of the regenerated silk fibroin solution is 20wt%.The mass fraction of the graphene oxide dispersion is
10%.The salt is lithium bromide, calcium chloride, the mixture of magnesium chloride.
The present invention can realize the dissolving of fibroin nanometer fibril level, reduce the destruction to fibroin molecular structure, pass through oxygen
The addition of graphite alkene improves hydrogel mechanical property, be it is a kind of it is simple, effectively, the method for preparing strong mechanical performance hydrogel.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair
Equivalent structure or equivalent flow conversion that bright description is made, or directly or indirectly it is used in other related technology necks
Domain, is included within the scope of the present invention.
Claims (4)
1. a kind of preparation method of the regenerated silk fibroin hydrogel with graphene oxide, it is characterised in that including following step
Suddenly：
The salt that concentration is 1.0 ~ 10.0 wt% is mixed with the formic acid that concentration is 10 ~ 98 wt%, A liquid is designated as；
Boiled silk is put into A liquid, after boiled silk dissolving, namely regenerated silk fibroin protein solution；
Regenerated silk fibroin solution and graphene oxide dispersion are pressed into (7.5 ~ 9.5)：The ratio mixing of (2.5 ~ 0.5), and fill
Divide stirring 3 ~ 10 minutes, be designated as B liquid；
B liquid is put into climatic chamber, the temperature of climatic chamber is set as 30 ~ 50 DEG C, and humidity set is 60 ~ 70%RH, is made
Its natural gel, you can obtaining has graphene oxide and regenerated silk fibroin hydrogel.
2. a kind of preparation method of regenerated silk fibroin hydrogel with graphene oxide according to claim 1, its
It is characterised by：The concentration of the regenerated silk fibroin solution is 10 ~ 30wt%.
3. a kind of preparation method of regenerated silk fibroin hydrogel with graphene oxide according to claim 1, its
It is characterised by：The mass fraction of the graphene oxide dispersion is 10 ~ 11%.
4. a kind of preparation method of regenerated silk fibroin hydrogel with graphene oxide according to claim 1, its
It is characterised by：The salt is one kind or wherein any several in lithium bromide, calcium chloride, magnesium chloride, lithium rhodanate, sodium sulfocyanate
Mixture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710366264.2A CN107189080A (en) | 2017-05-23 | 2017-05-23 | A kind of preparation method of the regenerated silk fibroin hydrogel with graphene oxide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710366264.2A CN107189080A (en) | 2017-05-23 | 2017-05-23 | A kind of preparation method of the regenerated silk fibroin hydrogel with graphene oxide |
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| Publication Number | Publication Date |
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| CN107189080A true CN107189080A (en) | 2017-09-22 |
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| CN201710366264.2A Pending CN107189080A (en) | 2017-05-23 | 2017-05-23 | A kind of preparation method of the regenerated silk fibroin hydrogel with graphene oxide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109054047A (en) * | 2018-05-24 | 2018-12-21 | 华中科技大学同济医学院附属协和医院 | A kind of silk gum/graphene oxide composite hydrogel and its preparation method and application |
| CN112940293A (en) * | 2021-02-25 | 2021-06-11 | 中国科学院上海微***与信息技术研究所 | Preparation method of hydrogel based on biological protein |
| CN113252081A (en) * | 2021-05-12 | 2021-08-13 | 电子科技大学 | Flexible composite sensor based on fibroin and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103572395A (en) * | 2013-11-14 | 2014-02-12 | 东华大学 | Strengthened toughened regenerated silk fibers and preparation method thereof |
| KR101427731B1 (en) * | 2013-06-04 | 2014-09-22 | 인하대학교 산학협력단 | Manufacturing method of carbon aerogels |
| CN104107681A (en) * | 2014-06-18 | 2014-10-22 | 同济大学 | Preparation method of three-dimensional graphene-protein composite aerogel |
| CN106048765A (en) * | 2016-07-14 | 2016-10-26 | 苏州经贸职业技术学院 | Preparation method of artificial ligament material |
-
2017
- 2017-05-23 CN CN201710366264.2A patent/CN107189080A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101427731B1 (en) * | 2013-06-04 | 2014-09-22 | 인하대학교 산학협력단 | Manufacturing method of carbon aerogels |
| CN103572395A (en) * | 2013-11-14 | 2014-02-12 | 东华大学 | Strengthened toughened regenerated silk fibers and preparation method thereof |
| CN104107681A (en) * | 2014-06-18 | 2014-10-22 | 同济大学 | Preparation method of three-dimensional graphene-protein composite aerogel |
| CN106048765A (en) * | 2016-07-14 | 2016-10-26 | 苏州经贸职业技术学院 | Preparation method of artificial ligament material |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109054047A (en) * | 2018-05-24 | 2018-12-21 | 华中科技大学同济医学院附属协和医院 | A kind of silk gum/graphene oxide composite hydrogel and its preparation method and application |
| CN112940293A (en) * | 2021-02-25 | 2021-06-11 | 中国科学院上海微***与信息技术研究所 | Preparation method of hydrogel based on biological protein |
| CN113252081A (en) * | 2021-05-12 | 2021-08-13 | 电子科技大学 | Flexible composite sensor based on fibroin and preparation method thereof |
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