CN103638554B - A kind of fibroin albumen and the preparation method of marine mussel adhesion protein composite - Google Patents
A kind of fibroin albumen and the preparation method of marine mussel adhesion protein composite Download PDFInfo
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- CN103638554B CN103638554B CN201310562721.7A CN201310562721A CN103638554B CN 103638554 B CN103638554 B CN 103638554B CN 201310562721 A CN201310562721 A CN 201310562721A CN 103638554 B CN103638554 B CN 103638554B
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- adhesion protein
- mussel adhesion
- marine mussel
- fibroin albumen
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Abstract
The present invention relates to the preparation method of a kind of fibroin albumen and marine mussel adhesion protein composite, disclose a kind of preparation method of fibroin albumen multifunctional material compound with marine mussel adhesion protein, preparation process is that regenerated silk fibroin and a certain proportion of marine mussel adhesion protein are dissolved in a certain amount of organic solvent, being stirred continuously makes its mix homogeneously, it is made into finite concentration, can be prepared by the mixed solution of fibroin albumen and marine mussel adhesion protein, a certain amount of mixed solution masking or support.The present invention has power consumption low in whole processing procedure, biological safety height, cheap, simple to operation, the advantage such as environmentally safe；There is stronger mechanical property by biological fibroin obtained in the invention with marine mussel adhesion protein composite nano-fiber membrane.
Description
Technical field
The present invention relates to a kind of fibroin albumen and the preparation method of marine mussel adhesion protein composite, belong to fibroin egg
White processing method.
Background technology
Silkworm albumen, tussah silk fibroin are fibrin, because of its unique mechanical property and good bio-compatible
Property, degradability and stability, and its essence is the construction featuress of protein, is widely used in the fields such as medicine, biomaterial, is
A kind of preferable bone renovating bracket material.And the mechanical property of excellent cell adhesion properties and high intensity is bone repairing support
The necessary requirement of material.
Marine mussel adhesion protein has high intensity, high tenacity and water proofing property, and the extremely strong function of sticking matrix, this
Special with which molecular structure, DOPA（DOPA）The interchain linkage of mediation and the interaction mode between ground are relevant.Make a gift of
Shellfish attachment proteinses are a series of alkaline proteins, and the aminoacid of hydroxyl is up to 60%-70%, wherein proline（Pro）Most of
Translated rear hydroxylating is modified to urinary hydroxyproline（Hyp）, stablize the phase interaction between polyphenol protein and its between tropocollagen molecule
With.DOPA（DOPA）Middle catechol group has very strong metal chelation abilities, can form reversible organic metal with metal
Complex, and covalent bond can be formed with a lot of radical reactions after catechol is oxidized to quinone.This strong covalent bond of DOPA
And coordination ability is the main cause that mussel adhesion protein has superpower adhesive force.Further, it also has good bio-compatible
Property and degradability waterproof and avirulence, are the biological adhesives of class very advantageous and potentiality, therefore in soft tissue bonding, people
Make the field such as skeleton, knochenbruch engagement, Oral Repair, ophthalmologic operation and shipbuilding, coating to be with a wide range of applications.
Biopolymers companies start the bonding for directly extracting and being developed into tissue culture from mussel pedal gland from the eighties in 20th century
Agent product " Cell-Tak ", the product are mainly the mixture of mfp-1, mfp-2 and mfp-3 in mussel attachment proteinses, can be used for
In cell cultivation process, non-adherent cell and culture dish sticks, it can be seen that, marine mussel adhesion protein is viscous to Materials Cell
The impact of attached function.
When regenerated silk and marine mussel adhesion protein mixed dissolution are in organic solvent or aqueous solution, marine mussel glues
Attached albumen is crosslinked by its strong covalent bond and fibroin albumen, significantly improves the mechanical property of silk fibroin composite material；And sea
Foreign mussel attachment proteinses can improve the biocompatibility of silk fibroin composite material, improve adhesive capacity of the composite to cell.
Content of the invention
A kind of improvement of the present invention exactly done by the shortcomings of the prior art, there is provided technique letter
Single, process preparation method convenient and that the fibroin albumen multifunctional material compound with marine mussel adhesion protein can be provided.
To achieve these goals, concrete technical scheme of the invention is as follows：
The invention discloses a kind of preparation method of fibroin albumen multifunctional material compound with marine mussel adhesion protein,
Concrete preparation process is as follows：
（1）, regenerated silk fibroin and a certain proportion of marine mussel adhesion protein be dissolved in a certain amount of organic solvent
In, being stirred continuously makes its mix homogeneously, is made into finite concentration, you can the mixing of fibroin albumen and marine mussel adhesion protein is obtained
Solution；
（2）, take a certain amount of mixed solution masking or support.
Used as further improvement, the present invention substitutes the organic solvent in claim 1 with aqueous solution.
Used as further improvement, the present invention prepares Nanowire by electrostatic spinning technique with taking a certain amount of mixed solution
Dimension film is substituted and takes a certain amount of mixed solution masking or support.
Used as further improvement, fibroin albumen of the present invention is regeneration silk fibroin protein or regeneration tussah silk peptide
Albumen.
Used as further improvement, organic solvent of the present invention is hexafluoroisopropanol or Hexafluoro acetone or formic acid or three
Fluoroethanoic acid.
Used as further improvement, the thin film or support made by the present invention has good biological activity and compatible
Property, and there is some superiority to cell adhesion.
Used as further improving, fibroin albumen of the present invention passes through electrostatic with marine mussel adhesion protein composite
Nano fibrous membrane made by spining technology passes through the multiple of covalent bond/non-covalent bond with the DOPA in marine mussel adhesion protein
Interact, the mechanical property of composite can be effectively improved.
Compared with prior art, the present invention has following prominent characteristics：
(1) present invention has power consumption low in whole processing procedure, biological safety height, cheap, side simple to operate
Just, the advantage such as environmentally safe；
(2) have very well by biological fibroin obtained in the invention and marine mussel adhesion protein composite membrane and timbering material
Biocompatibility.Because marine mussel adhesion protein contains a certain amount of DOPA（DOPA）, and the phenolic group in DOPA molecules
The non-covalent bonding formed between oxidation and the DOPA molecules for thus being mediated and the extraneous surface of solids couples and has strong viscous
Conjunction property.So the composite containing certain proportion marine mussel adhesion protein has extraordinary cell adhesion properties；
(3) have with marine mussel adhesion protein composite nano-fiber membrane by biological fibroin obtained in the invention stronger
Mechanical property.Because the DOPA in marine mussel adhesion protein is multiple mutual with silk fibroin material by covalent bond/non-covalent bond
Effect, can improve the mechanical property of nano fibrous membrane.
Specific embodiment
The present invention is mainly implemented by the following two kinds of programs：
（1）By regenerated silk fibroin and a certain proportion of marine mussel adhesion protein be dissolved in a certain amount of organic solvent or
In aqueous solution, being stirred continuously makes its mix homogeneously, is made into finite concentration, you can fibroin albumen and marine mussel adhesion protein is obtained
Mixed solution.Take a certain amount of mixed solution masking, support or nano fibrous membrane is prepared by electrostatic spinning technique.
（2）Regenerated silk is dissolved in a certain amount of organic solvent or aqueous solution, finite concentration is made into.Take a certain amount of
Mixed solution masking, support prepare nano fibrous membrane by electrostatic spinning technique.Then by the film that makes, support and nanometer
Fibrous membrane is soaked in certain density marine mussel adhesion protein solution, takes out and dry after certain time.
Technical scheme is described in further detail below by specific embodiment, following examples are right
The present invention explanation and the invention is not limited in following examples.
Embodiment 1
The marine mussel adhesion protein powder of regeneration silk fibroin protein 1g and 10mg is weighed, is placed in flat bottom glass bottle,
A certain amount of hexafluoroisopropanol solution is added, concentration is made into for 7.5% solution, is stirred continuously after its mix homogeneously, take out 7ml and apply
Naturally dry on diameter 10cm plastic plates.Tested by cell experiment, regenerated silk is compound with marine mussel adhesion protein
Film, timbering material are stronger than single regenerated silk Materials Cell adhesion property.
Embodiment 2
The marine mussel adhesion protein powder of regeneration silk fibroin protein 1g and 10mg is weighed, is placed in flat bottom glass bottle,
A certain amount of hexafluoroisopropanol solution is added, and concentration is made into for 7.5% solution, is stirred continuously after its mix homogeneously, by mixed solution
As for Electrospun is carried out in electrostatic spinning device, obtaining nano fibrous membrane carries out Mechanics Performance Testing, this viscous containing marine mussel
The composite electrostatic spinning film of attached albumen is eager to excel than single fibroin electrostatic spinning film dynamic performance.
Embodiment 3
Regeneration silk fibroin protein 1g is weighed, is placed in flat bottom glass bottle, add a certain amount of formic acid solution, being made into concentration is
10% solution, after its mix homogeneously, takes out 7ml and is applied on diameter 10cm plastic plates and dry naturally；Or make propping up for certain pattern
Frame material；Or mixed solution is obtained nano fibrous membrane as Electrospun is carried out in electrostatic spinning device；Again by this composite membrane,
Support, nano fibrous membrane are soaked in 24h in 10% marine mussel adhesion protein solution, take out nature afterwards and dry, test respectively
Cell experiment and mechanical property.
Finally, in addition it is also necessary to it is noted that listed above is only several specific embodiments of the invention.Obviously, the present invention
Above example is not limited to, there can also be many deformations.One of ordinary skill in the art can be straight from present disclosure
The all deformations that derives or associate are connect, protection scope of the present invention is considered as.
Claims (5)
1. a kind of preparation method of the compound multifunctional material of fibroin albumen and marine mussel adhesion protein, it is characterised in that tool
Preparation step is as follows：
(1), regenerated silk fibroin and a certain proportion of marine mussel adhesion protein are dissolved in a certain amount of organic solvent, no
Disconnected stirring makes its mix homogeneously, is made into finite concentration, you can the mixed solution of fibroin albumen and marine mussel adhesion protein is obtained；
(2), take a certain amount of mixed solution and nano fibrous membrane is prepared by electrostatic spinning technique.
2. the preparation side of the compound multifunctional material of fibroin albumen according to claim 1 and marine mussel adhesion protein
Method, it is characterised in that substitute the organic solvent in claim 1 with aqueous solution.
3. the preparation of the fibroin albumen according to claim 1 and 2 multifunctional material compound with marine mussel adhesion protein
Method, it is characterised in that described fibroin albumen is regeneration silk fibroin protein or regeneration tussah silk fibroin.
4. the preparation side of the compound multifunctional material of fibroin albumen according to claim 2 and marine mussel adhesion protein
Method, it is characterised in that described organic solvent is hexafluoroisopropanol or Hexafluoro acetone or formic acid or trifluoroacetic acid.
5. the preparation side of the compound multifunctional material of fibroin albumen according to claim 1 and marine mussel adhesion protein
Method, it is characterised in that made nano fibrous membrane has stronger mechanical property.
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| CN105088201B (en) * | 2014-05-14 | 2017-11-21 | 北京纳通科技集团有限公司 | The magnesium or surface treatment method of Mg alloy of a kind of controllable degradation speed |
| CN105327398A (en) * | 2014-08-07 | 2016-02-17 | 北京纳通科技集团有限公司 | Antimicrobial coating composition, medical implant material containing antimicrobial coating and preparation method of medical implant material |
| CN105617446B (en) * | 2014-10-30 | 2019-06-07 | 北京纳米能源与***研究所 | A kind of hemostasis composite material and preparation method |
| WO2017101027A1 (en) * | 2015-12-15 | 2017-06-22 | 江阴市本特塞缪森生命科学研究院有限公司 | Modified biomedical material product |
| WO2017101022A1 (en) * | 2015-12-15 | 2017-06-22 | 江阴市本特塞缪森生命科学研究院有限公司 | Modified medical scaffold material |
| CN105457085B (en) * | 2016-01-25 | 2018-03-02 | 华中农业大学 | A kind of bionical adhesion hydrogel of collagen-based mussel and preparation method thereof |
| CN108744023B (en) * | 2018-06-15 | 2021-04-27 | 福州大学 | Silk fibroin medical biological adhesive and preparation method thereof |
| CN108744055B (en) * | 2018-06-15 | 2021-04-27 | 福州大学 | Silk fibroin bone cement biological adhesive and preparation method thereof |
| CN110711264B (en) * | 2018-06-26 | 2022-08-23 | 杨佼佼 | Composite material, medical adhesive, and preparation method and application thereof |
| CN111995777B (en) * | 2019-05-31 | 2022-06-03 | 中国科学院青岛生物能源与过程研究所 | Preparation method of PEGDA-mussel adhesive protein-collagen composite hydrogel with strong adhesion and high mechanical strength |
| CN111991608B (en) * | 2020-08-27 | 2021-10-01 | 振德医疗用品股份有限公司 | Wound surface covering and preparation method thereof |
| CN115887730B (en) * | 2022-03-22 | 2024-03-12 | 德晟康(苏州)生物科技有限公司 | Microsphere suitable for hydrocolloid dressing, preparation method thereof and compound protein slow-release hydrocolloid dressing |
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| CN100551449C (en) * | 2006-12-30 | 2009-10-21 | 苏州大学 | Antheraea pernyi silk fibrion biology medicine material and preparation method thereof |
| CN101498061B (en) * | 2009-03-05 | 2011-08-03 | 浙江大学 | Method for improving antibiosis of silk fibroin material |
| KR101384746B1 (en) * | 2011-08-24 | 2014-04-14 | 포항공과대학교 산학협력단 | Surface immobilization of various functional biomolecules using mussel adhesive protein |
| KR101351119B1 (en) * | 2012-01-13 | 2014-01-15 | 포항공과대학교 산학협력단 | Mussel adhesive protein-added brushited bone graft substitute |
| CN103087976A (en) * | 2013-01-05 | 2013-05-08 | 高敏 | Material for promoting cell growth adhering to wall and preparation method of material |
| CN103272267A (en) * | 2013-06-07 | 2013-09-04 | 浙江大学 | Preparation method of silk fibroin and Fe3O4 composite material |
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