CN107164454A - Benza microbial limit tests - Google Patents
Benza microbial limit tests Download PDFInfo
- Publication number
- CN107164454A CN107164454A CN201710531728.0A CN201710531728A CN107164454A CN 107164454 A CN107164454 A CN 107164454A CN 201710531728 A CN201710531728 A CN 201710531728A CN 107164454 A CN107164454 A CN 107164454A
- Authority
- CN
- China
- Prior art keywords
- benza
- filter
- test
- flushing
- microbial limit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/14—Streptococcus; Staphylococcus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/305—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F)
- G01N2333/31—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F) from Staphylococcus (G)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/32—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Bacillus (G)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
- G01N2333/38—Assays involving biological materials from specific organisms or of a specific nature from fungi from Aspergillus
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of Benza microbial limit tests, comprise the following steps：Benza is taken, dilution is added, is made 1:10 test liquids；1 part of test liquid is drawn with sterile pipette tip, is added in 50 parts of dilutions, is mixed；First with a small amount of flushing liquor rinse low absorbability filter membrane, then membrane-filter procedure is used to filter test liquid full dose after dilution, with flushing liquor gradation flushing filter membrane, each flushing dose is the 1/16 of total flushing dose；Two filter membranes are prepared with method, are affixed on respectively on two kinds of different culture mediums, after suitable condition culture, point meter clump count；Negative control：Dilution is filtered using membrane-filter procedure, bacterium is affixed on culture medium and cultivated up.The test method science of the present invention, result of the test is accurate, effectively reduces the biocidal property of Benza, has filled up the blank of benzalkonium chloride raw material microbial limit tests, had broad application prospects.
Description
Technical field
The present invention relates to enzyme or the measure or the method for inspection of microorganism, specifically a kind of Benza microbial limit
Inspection method.
Background technology
Benza is the mixture of chlorodimethyl benzylic hydrogens amine, colourless or flaxen supernatant liquid, gas virtue
Perfume (or spice), taste is extremely bitter, and a large amount of foams can be produced during shaking, for pre-surgical skin disinfection, mucous membrane and wound disinfection.
Benzalkonium chloride is cationic surfactant, and wide-spectrum bactericide acts on stronger, in pharmacy to gram-positive bacterium
Used in engineering as preservative.Because benzalkonium chloride raw material bacteriostasis is big, deposited in design microorganism limit test method
Great difficult.
The content of the invention
The present invention is exactly the problem of limit test of microbe is difficult, institute in order to solve benzalkonium chloride raw material bacteriostasis greatly
A kind of Benza microbial limit tests proposed.
The present invention is realized according to following technical scheme.
A kind of Benza microbial limit tests, comprise the following steps：
I, takes Benza, adds dilution, is made 1:10 test liquids；
II, draws 1 part of test liquid with sterile pipette tip, is added in 50 parts of dilutions, mixes；
Test liquid full dose mistake after III, will first be diluted with a small amount of flushing liquor rinse low absorbability filter membrane, then use membrane-filter procedure
Filter, filter membrane is rinsed with flushing liquor by several times, and each flushing dose is the 1/16 of total flushing dose；
IV, prepares two filter membranes with method, is affixed on respectively on two kinds of different culture mediums, after suitable condition culture, point meter bacterium colony
Number；
V, negative controls：Dilution is filtered using membrane-filter procedure, bacterium is affixed on culture medium and cultivated up.
The composition of the dilution and flushing liquor is peptone dry powder and Tween-80, and the quality parts ratio of the two is 1:1.
The preparation method of the dilution and flushing liquor is：Claim 5 parts of peptone dry powder, 5 parts of Tween-80, be dissolved in 1000 parts
In water, pH is adjusted to make the pH value after sterilizing for 7.1 ± 0.2,115-121 DEG C of autoclaving 15-30 minutes.
Total flushing dose is 800ml.
The culture medium is pancreas junket soya peptone agar medium and Sabouraud glucose agar；Pancreas junket soya peptone agar
Culture is cultivated 3 days based on 30-35 DEG C, and Sabouraud glucose agar is cultivated 5 days in 20-25 DEG C.
After the negative control dilution filtering, bacterium is affixed on pancreas junket soya peptone agar medium up, 30-35 DEG C of training
Support 3 days.
Present invention obtains following beneficial effect.
The effective benzalkonium chloride raw material bacteriostasis that solves of the invention is big, the problem of limit test of microbe is difficult.This
Invention passes through early stage a large amount of method of counting applicability Primary Screening Tests, it is determined that dilution, flushing liquor and dilution, flushing liquor
Amount, and result of the test improves the accuracy of testing result with " cfu/ml " calculating.The test method science of the present invention, experiment
As a result it is accurate, the blank of benzalkonium chloride raw material microbial limit tests has been filled up, has been had broad application prospects.
Embodiment
First, experiment prepares：
1. test sample：Benza（10%）
2. strain：
Staphylococcus aureus（CMCC（B）26003）
Pseudomonas aeruginosa（CMCC（B）10104）
Bacillus subtilis（CMCC（B）63501）
Candida albicans（CMCC（F）98001）
Black-koji mould（CMCC（F）98003）
Above reference culture is purchased from Nat'l Pharmaceutical ＆ Biological Products Control Institute or Tianjin City Medicine Inspection Inst..
3. culture medium（Refer to table 1）
4. culture medium, flushing liquor, neutralization agent compounding method（Refer to table 2）
5. test apparatus（Refer to table 3）
6. the preparation of bacteria suspension：
A. bacterium is increased：Inoculation staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis are trained in pancreas junket soya peptone agar respectively
Support on base flat board, 30-35 DEG C of insulating box culture 18-24 hours；Candida albicans, black-koji mould are inoculated with Sabouraud's dextrose agar
In culture medium, 20-25 DEG C of insulating box culture, Candida albicans culture 24-48 hours, black-koji mould 5-7 days is contained with 3-5ml
0.9% sterile NaCl solution of 0.05% Tween 80 elutes aspergillus niger spore, is received with capillary of the mouth of pipe with sterile absorbent cotton
Collect spore suspension to preserve in sterile test tube, it is standby.
B. bacterium solution dilutes：Staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis, Candida albicans, aspergillus niger
With 0.9% sterile NaCl solution, by 10 times of dilutions, experiment bacterium solution is made in the fresh cultured thing of bacterium；Test every milliliter of bacteria containing amount of bacterium solution
Less than 100cfu.
C. freshly prepd bacteria suspension should be used in 2 hours at ambient temperature, and 2-8 DEG C of refrigeration can be preserved 24 hours.
2nd, the foundation of test method
《Chinese Pharmacopoeia》Regulation：The microbial enumeration method of test sample should carry out method applicability experiment, to confirm what is used
Method is suitable for the microorganism count of the product.
Method applicability experiment needs to prove dilution, flushing liquor in itself on the growth of test organisms without influence, and can
Antipathogenic composition is substantially reduced, therefore needs to investigate dilution, the toxicity and effect of flushing liquor.Because the benzalkonium chloride of quaternary ammonium salt is molten
Liquid to the bactericidal effect of gram-positive bacteria substantially, it is poor to Gram-negative bacteria lethality, and can not kill bacterial spore and
Fungal spore, therefore selection staphylococcus aureus is used as test liquid control group test strain.
1. it is prepared by test liquid
Take Benza（10%）10ml, with the 0.5% peptone water solution containing 0.5% Tween-80 as diluent, is made
1:10 test liquids.
2. test operation
2.1 test group
Take the test liquid 10ml prepared to be added in 0.5% peptone water solution of the 500ml containing 0.5% Tween-80, mix, plus
Enter to be less than 100cfu bacterium solution.After the 0.5% peptone water solution rinse filter membrane containing 0.5% Tween-80, the confession of bacterium solution will be added
Test solution full dose is filtered, and is rinsed by several times with the same flushing liquors of 800ml, each 50ml/ films.Each test organisms is operated with method.
Remove filter membrane.Staphylococcus aureus, pseudomonas aeruginosa, the film of bacillus subtilis are affixed on pancreas junket soybean respectively
On peptone agar medium, 30-35 DEG C of pancreas junket soya peptone agar medium is cultivated 3 days；Candida albicans, the film of black-koji mould are respectively made
It is standby 2 parts, it is affixed on respectively on pancreas junket soya peptone agar and Sabouraud glucose agar.Pancreas junket soya peptone agar medium 30-
35 DEG C are cultivated 5 days, and 20-25 DEG C of Sabouraud glucose agar is cultivated 5 days（Following condition of culture is identical）.
2.2 test liquid control groups
Experiment bacterium solution is replaced to prepare the dilution of bacterium solution, with test group operation, two films is prepared, trypticase soybean is affixed on respectively
Agar medium（TSA）And Sabouraud glucose agar（SDA）On, relevant temperature culture determines its background bacterium number.
2.3 bacterium solution control groups
Take 0.5% peptone water solution of the 10ml without Tween-80 to replace test liquid, be added in 500ml dilutions, addition is less than
100cfu tests bacterium solution, full dose filtering, to determine added bacteria suspension aerobic plate count.Each test organisms is operated with method.
2.4 nertralizer groups
0.5% peptone water solution of the 10ml containing 0.5% Tween-80 is taken to replace test liquid, with test group operation, each test organisms is same
Method is operated, to confirm its validity and the nontoxicity to microorganism.
3. method applicability result of the test（Refer to table 4）
4. conclusion
Above employment and suitability test (E ＆ ST) result shows:Each test group clump count rate of recovery（I.e.（Test group-test sample control group）Bacterium colony
Number/bacterium solution group clump count）In the range of 0.5-2；The nertralizer control group bacterium number rate of recovery（Nertralizer control group clump count/bacterium solution
Group clump count）In the range of 0.5-2.So the test method can be used for the limit test of microbe of the test sample.
5. daily microbial limit tests are established
Take Benza（10%）10ml, adds the 0.5% peptone water solution containing 0.5% Tween-80, is made 1:10 for examination
Liquid.10ml is drawn with sterile pipette tip（Equivalent to test sample 1ml Benzas）, it is added to 500ml and contains 0.5% Tween-80
In 0.5% peptone water solution, mix.First filtered with a small amount of 0.5% peptone water solution flushing liquor rinse containing 0.5% Tween-80
Film, then filtered test liquid full dose using membrane-filter procedure, rinsed by several times with 800ml flushing liquors, each 50ml.Two are prepared with method
Filter membrane, be affixed on respectively in pancreas junket soya peptone agar medium and Sabouraud glucose agar plate on, pancreas junket soya peptone
30-35 DEG C of agar ware is cultivated for 3 days；20-25 DEG C of Sabouraud's dextrose agar ware is cultivated for 5 days, point meter clump count.
Negative control：Dilution 50ml is taken, is filtered, bacterium is affixed on pancreas junket soya peptone agar plates up, 30-35 DEG C
Culture 3 days, must not there is bacteria growing.
Claims (6)
1. a kind of Benza microbial limit tests, it is characterised in that：Comprise the following steps：
I, takes Benza, adds dilution, is made 1:10 test liquids；
II, draws 1 part of test liquid with sterile pipette tip, is added in 50 parts of dilutions, mixes；
Test liquid full dose mistake after III, will first be diluted with a small amount of flushing liquor rinse low absorbability filter membrane, then use membrane-filter procedure
Filter, filter membrane is rinsed with flushing liquor by several times, and each flushing dose is the 1/16 of total flushing dose；
IV, prepares two filter membranes with method, is affixed on respectively on two kinds of different culture mediums, after suitable condition culture, point meter bacterium colony
Number；
V, negative controls：Dilution is filtered using membrane-filter procedure, bacterium is affixed on culture medium and cultivated up.
2. a kind of Benza microbial limit tests according to claim 1, it is characterised in that：It is described dilute
The composition for releasing liquid and flushing liquor is peptone dry powder and Tween-80, and the quality parts ratio of the two is 1:1.
3. a kind of Benza microbial limit tests according to claim 2, it is characterised in that：It is described dilute
The preparation method for releasing liquid and flushing liquor is：Claim 5 parts of peptone dry powder, 5 parts of Tween-80, be dissolved in 1000 parts of water, adjust pH to make to go out
PH value after bacterium is 7.1 ± 0.2,115-121 DEG C of autoclaving 15-30 minutes.
4. a kind of Benza microbial limit tests according to claim 1, it is characterised in that：It is described total
Flushing dose is 800ml.
5. a kind of Benza microbial limit tests according to claim 1, it is characterised in that：The training
It is pancreas junket soya peptone agar medium and Sabouraud glucose agar to support base；Pancreas junket soya peptone agar medium is in 30-35
DEG C culture 3 days, Sabouraud glucose agar in 20-25 DEG C cultivate 5 days.
6. a kind of Benza microbial limit tests according to claim 1, it is characterised in that：Described the moon
Property control dilution filtering after, bacterium is affixed on pancreas junket soya peptone agar medium up, 30-35 DEG C cultivate 3 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710531728.0A CN107164454A (en) | 2017-07-03 | 2017-07-03 | Benza microbial limit tests |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710531728.0A CN107164454A (en) | 2017-07-03 | 2017-07-03 | Benza microbial limit tests |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107164454A true CN107164454A (en) | 2017-09-15 |
Family
ID=59827906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710531728.0A Pending CN107164454A (en) | 2017-07-03 | 2017-07-03 | Benza microbial limit tests |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107164454A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109777856A (en) * | 2018-12-20 | 2019-05-21 | 武汉科诺生物科技股份有限公司 | A kind of detection method of 1,000,000,000,000 live spores per gram Bacillus subtillis original powder brood cell's number living |
| CN110693754A (en) * | 2019-11-16 | 2020-01-17 | 烟台东方化学有限公司 | Antibacterial and anti-inflammatory pyridone composition and cosmetic containing same |
| CN111850089A (en) * | 2019-04-28 | 2020-10-30 | 成都倍特药业股份有限公司 | Method and composition for checking microbial limit of bacteriostatic beta receptor agonist drug |
| CN113046410A (en) * | 2021-04-07 | 2021-06-29 | 费森尤斯卡比华瑞制药有限公司 | Microbial limit inspection method of disodium ethylene diamine tetraacetate |
| CN116751837A (en) * | 2023-08-14 | 2023-09-15 | 湖南新领航检测技术有限公司 | Sterile detection method of rifampicin for injection |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004032980A1 (en) * | 2002-10-04 | 2004-04-22 | Elan Pharma International Limited | Gamma irradiation of solid nanoparticulate active agents |
| CN102586393A (en) * | 2012-02-24 | 2012-07-18 | 陕西省食品药品检验所 | Microbial limit detection method of miconazole nitrate suppository |
| CN102697713A (en) * | 2012-06-14 | 2012-10-03 | 浙江尖峰药业有限公司 | Sodium hyaluronate eye drops and a preparation method thereof |
| CN106222237A (en) * | 2016-08-05 | 2016-12-14 | 中国大冢制药有限公司 | Gatifloxacin eye drop Sterility Test Methods |
-
2017
- 2017-07-03 CN CN201710531728.0A patent/CN107164454A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004032980A1 (en) * | 2002-10-04 | 2004-04-22 | Elan Pharma International Limited | Gamma irradiation of solid nanoparticulate active agents |
| CN102586393A (en) * | 2012-02-24 | 2012-07-18 | 陕西省食品药品检验所 | Microbial limit detection method of miconazole nitrate suppository |
| CN102697713A (en) * | 2012-06-14 | 2012-10-03 | 浙江尖峰药业有限公司 | Sodium hyaluronate eye drops and a preparation method thereof |
| CN106222237A (en) * | 2016-08-05 | 2016-12-14 | 中国大冢制药有限公司 | Gatifloxacin eye drop Sterility Test Methods |
Non-Patent Citations (5)
| Title |
|---|
| GERBA CHARLES P: "Quaternary Ammonium Biocides: Efficacy in Application", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 * |
| 中华人民共和国国家质量监督检验检疫总局: "《中华人民共和国国家标准GB/T 24404-2009/ISO 21149：2006》", 30 September 2009, 中国标准出版社 * |
| 吴晓玲等: "薄膜过滤法中冲洗量与冲洗次数对实验结果的影响", 《海峡药学》 * |
| 国家药典委员会: "《中华人民共和国药典 2015年版 二部》", 30 June 2015, 中国医药科技出版社 * |
| 林小晖等: "苯扎氯铵贴薄膜过滤无菌检查方法的建立及验证", 《中外食品工业》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109777856A (en) * | 2018-12-20 | 2019-05-21 | 武汉科诺生物科技股份有限公司 | A kind of detection method of 1,000,000,000,000 live spores per gram Bacillus subtillis original powder brood cell's number living |
| CN111850089A (en) * | 2019-04-28 | 2020-10-30 | 成都倍特药业股份有限公司 | Method and composition for checking microbial limit of bacteriostatic beta receptor agonist drug |
| CN110693754A (en) * | 2019-11-16 | 2020-01-17 | 烟台东方化学有限公司 | Antibacterial and anti-inflammatory pyridone composition and cosmetic containing same |
| CN110693754B (en) * | 2019-11-16 | 2022-08-23 | 烟台东方化学有限公司 | Antibacterial and anti-inflammatory pyridone composition and cosmetic containing same |
| CN113046410A (en) * | 2021-04-07 | 2021-06-29 | 费森尤斯卡比华瑞制药有限公司 | Microbial limit inspection method of disodium ethylene diamine tetraacetate |
| CN116751837A (en) * | 2023-08-14 | 2023-09-15 | 湖南新领航检测技术有限公司 | Sterile detection method of rifampicin for injection |
| CN116751837B (en) * | 2023-08-14 | 2023-11-07 | 湖南新领航检测技术有限公司 | Sterile detection method of rifampicin for injection |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107164454A (en) | Benza microbial limit tests | |
| Halkai et al. | Evaluation of antibacterial efficacy of fungal-derived silver nanoparticles against Enterococcus faecalis | |
| US10221440B2 (en) | Method for determining the sensitivity of microorganisms to antimicrobial substances | |
| Kusuma et al. | Antimicrobial spectrum of red Piper betel leaf extract (Piper crocatum Ruiz & Pav) as natural antiseptics against airborne pathogens | |
| CN110151752A (en) | A kind of tea polyphenols composition and its preparing the application in anti-streptococcus suis drug | |
| CN108300756A (en) | The microbial limit tests of Nifuratel capsule | |
| CN110199994A (en) | A kind of disinfectant and preparation method thereof, application, feed addictive | |
| CN113016798A (en) | Low-temperature bactericide | |
| CN116508792A (en) | Composite disinfectant | |
| CN105018395B (en) | One bacillus pumilus and its application in alternaria leaf spot of apple is prevented | |
| Tsukatani et al. | Rapid and simple determination of minimum biofilm eradication concentration by a colorimetric microbial viability assay based on reduction of a water-tetrazolium salt and combinated effect of antibiotics against microbial biofilm | |
| CN110229868A (en) | A kind of test method inhibiting Phytophthora nicotianae Breda | |
| CN104524558A (en) | Enzyme compounded preparation and preparation method thereof | |
| CN102939966B (en) | A kind of compound disinfectant, its preparation method and application | |
| CN115633697A (en) | Composition containing berberine, medical fertilizer, preparation method and application thereof | |
| CN106222237A (en) | Gatifloxacin eye drop Sterility Test Methods | |
| CN104721812A (en) | Lysostaphin compound enzyme disinfectant and preparation method thereof | |
| CN106879602A (en) | It is a kind of to cooperate with the composition for pressing down bacterium and antibacterium biofilm formation | |
| CN104322555A (en) | Preservative used for liquid washing product and preparation method thereof | |
| CN103798251B (en) | A kind of Recompounded pesticide and application thereof containing triazolone and the gloomy copper of thiophene | |
| CN112868657A (en) | Application of ethyl maltol as fungal inhibitor | |
| CN106118905A (en) | A kind of high-effective disinfecting liquid detergent | |
| Suresh et al. | Fluorescent pseudomonads contribute to the enhanced growth and yield of rice cultivated under system of rice intensification (SRI) | |
| CN113558059B (en) | Composition containing zhongshengmycin and natamycin | |
| CN101235047A (en) | Rare earth complex, preparation method and antibiotic application of the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170915 |