CN107158067B

CN107158067B - Traditional Chinese medicine composition for preventing and treating swine eperythrozoonosis and preparation method and application thereof

Info

Publication number: CN107158067B
Application number: CN201710257575.5A
Authority: CN
Inventors: 苗小楼; 尚小飞; 李芸; 潘虎; 王瑜; 王东升; 李冰
Original assignee: Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
Current assignee: Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
Priority date: 2017-04-19
Filing date: 2017-04-19
Publication date: 2021-02-26
Anticipated expiration: 2037-04-19
Also published as: CN107158067A

Abstract

The invention discloses a traditional Chinese medicine composition for preventing and treating swine eperythrozoonosis, which is prepared from the following raw materials in parts by weight: 3 parts of croton and 2 parts of liquorice; and provides a preparation method and application thereof. The invention has the beneficial effects that: the traditional Chinese medicine composition for preventing and treating eperythrozoonosis of pigs, provided by the invention, adopts Chinese herbal medicines as raw materials, has the effects of clearing heat, drying dampness, killing insects and removing accumulation through scientific compatibility, can effectively kill eperythrozoonosis, has a good treatment effect, has a cure rate of over 60 percent, avoids using chemical medicines and arsenic preparations in treatment of eperythrozoonosis of pigs, and avoids the problem of medicine residue in animal-derived food.

Description

Traditional Chinese medicine composition for preventing and treating swine eperythrozoonosis and preparation method and application thereof

Technical Field

The invention relates to the technical field of veterinary drug preparation, and particularly relates to a traditional Chinese medicine composition for preventing and treating swine eperythrozoonosis, and a preparation method and application thereof.

Background

Eperythrozoon disease is a zoonosis caused by Eperythrozoon infection of organism. Eperythrozoon is a type of microorganism that parasitizes on the surface of erythrocytes, plasma and bone marrow. Pig eperythrozoonosis is an acute and febrile infectious disease caused by attachment of pig eperythrozoonosis on the surface of pig red blood cells or free in blood plasma, and occurs and prevails in many countries and regions. Clinically, the disease is characterized by fever, anemia, hemolytic jaundice, dyspnea, skin redness and the like, and causes immunosuppression, reproductive failure and low survival rate of birth of animals. In recent years, the pig feed has explosive popularity in most provinces in China, and serious economic loss is caused to the pig industry. At present, the research on the immunization of the swine eperythrozoonosis vaccine is not broken through, and the specific drugs for the swine eperythrozoonosis are few. At present, terramycin, sulfanilamide, triazamidine, arsenic preparations and the like are generally adopted for preventing and treating eperythrozoonosis of the suckling pigs, and although certain effects are achieved, the cure rate is low, and the suckling pigs are easy to relapse. Some of them are even treated by arsenic preparations, although the arsenic preparations have certain curative effect, the environment is polluted, and toxic arsenic residues in pork endanger the health of consumers.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provides a traditional Chinese medicine composition for preventing and treating swine eperythrozoonosis and a preparation method and application thereof.

In order to achieve the purpose, the technical scheme provided by the invention is as follows: a traditional Chinese medicine composition for preventing and treating pig eperythrozoonosis is prepared from the following raw materials in parts by weight: 3 parts of croton and 2 parts of liquorice.

The second purpose of the invention is to provide a preparation method of the traditional Chinese medicine composition for preventing and treating the swine eperythrozoonosis, which is to crush croton and liquorice, pass through a third sieve and mix uniformly according to the proportion to prepare powder.

The third purpose of the invention is to provide the application of the traditional Chinese medicine composition for preventing and treating the swine eperythrozoonosis in the preparation of the medicine for preventing and treating the swine eperythrozoonosis.

Further, in the above application, the Chinese medicinal composition is added in a proportion of 2mg per kg body weight per day for 3 days continuously.

The invention has the beneficial effects that: the traditional Chinese medicine composition for preventing and treating eperythrozoonosis of pigs, provided by the invention, adopts Chinese herbal medicines as raw materials, has the effects of clearing heat, drying dampness, killing insects and removing accumulation through scientific compatibility, can effectively kill eperythrozoonosis, has a good treatment effect, has a cure rate of over 60 percent, avoids using chemical medicines and arsenic preparations in treatment of eperythrozoonosis of pigs, and avoids the problem of medicine residue in animal-derived food.

Detailed Description

Example 1:

mouse experiment for treating artificial infection with eperythrozoon:

test materials and methods:

1.1 test materials:

1.1.1 test animals: white mice of Kunming species, which are subjected to tail vein blood collection to prepare fresh blood pressure sheet microscopic examination, are determined to have no eperythrozoon infection, have the weight of 18-22 g and have half female and half male, and are purchased from the university medical college of Lanzhou.

1.1.2 test instruments and reagents:

light microscope (XSP-C104B), micropipette, sterile syringe, etc., PBS, saline. Test drugs: 6 parts of croton and 4 parts of liquorice are crushed and mixed uniformly to obtain the compound. Control drugs: amitraz, produced by lucent pharmaceutical company, orchids, lot number: 160412.

1.2 test methods:

for artificial infection, pig blood samples infected with eperythrozoon were washed 3 times with PBS and diluted with an equal volume of sterilized physiological saline to serve as infected samples. Mice were inoculated with 0.5 ml positive blood samples by intraperitoneal inoculation. Five days later, blood is collected through the caudal vein, physiological saline is added on a glass slide, a cover glass is covered, cedar oil is dripped, the glass is enlarged by 1000 times by using an oil scope for observation, red blood cells with thorns (which are called as teeth) are seen under the scope, or a plurality of semicircular or circular attachments (which are called as wolf tooth rods, lemon shapes and the like) are attached around the red blood cells, the red blood cells have slight vibration, the shrinkage, deformation and the like of the red blood cells, or the plasma has the condition that circular or unshaped moving objects with different sizes are seen, namely the infection is considered to be successful.

The experiments were divided into groups, and mice with successful infection were randomly divided into 5 groups of 10 mice each. Adding the medicine into the crushed rat grain, adding a proper amount of adhesive to prepare particles, and respectively preparing the rat grain with the medicine addition of 1 per mill, 2 per mill and 4 per mill; 1 group is a drug low-dose treatment group, and 4 g/mouse food with 1 per mill of drug addition is fed; 2 groups are drug medium dose treatment groups, and 4 g/mouse food with 2 per mill of drug addition is fed; 3 groups are drug high-dose treatment groups, and 4 g/mouse food with 4 per mill of drug addition is fed; 4 groups are amitraz treatment groups, which are injected intraperitoneally at a rate of 5.4mg/kg body weight per day; 5 groups were fed with mouse food without drug for three days.

2, evaluation index of curative effect:

change of infection rate mice were observed before the test, 20 visual fields were blood microscopically examined, the number of erythrocytes was recorded, and the infection rate of erythrocytes, i.e., (the number of erythrocytes infected with eperythrozoon/the number of erythrocytes) × 100%, was determined every day at regular time for each group after the administration of the drug, and the infection rate of erythrocytes was continuously measured for 6 days.

3 results and analysis:

the results of the drug treatment of the artificially infected eperythrozoon suis mice are shown in table 1.

TABLE 1

It can be seen from table 1 that the drug high, medium and low groups all had different degrees of therapeutic effect on the artificially infected mice, wherein the group with the high dose was best, close to the control drug, triazamidine, but the red blood cell infection rate decreased at a slower rate than the triazamidine treated group. The red blood cell infection rate of mice in the normal saline group is not changed, which indicates that the mice cannot self-heal after being infected with eperythrozoon.

Example 2:

clinical experiments are as follows:

1, materials and methods:

1.1 test materials:

the test animal selects 163 sick pigs (a suburban farm in the city of the Dingxi province) naturally infected with eperythrozoon, and the sick pigs are mainly characterized by inappetence and lassitude, the body temperature rises to 40-41 ℃, constipation occurs first, diarrhea occurs later, inappetence and dyspnea, eye and conjunctiva become flush first, then become pale and yellow, and skins of ears, limbs, under abdomen and the like have reddish purple spots. The blood of the sick pigs is subjected to microscopic examination, and eperythrozoon positive infected pigs are selected as test animals.

1.2 test methods: the test was divided into 2 groups, namely 80 heads for the triazamidine group and 83 heads for the drug group of the invention.

Triazamidine group: intramuscular injection, 6 mg/kg (body weight)/d, dissolving with normal saline, and continuously using for 3 days; the powder of the invention prepared by the method is added into the medicine group of the invention, 2 mg/kg (body weight)/d, and the medicine is taken for 3 days continuously.

2, judging the curative effect:

and (3) healing: clinical symptoms disappear, and no eperythrozoon is seen in the pig blood erythrocytes by the hanging drop microscopy.

Improvement: clinical symptoms disappear, and a small amount of eperythrozoon can be seen by the pig blood red blood cells through the hanging drop microscopic examination.

And (4) invalidation: clinical symptoms do not disappear, and the pig blood erythrocytes can be seen as reddish bodies by the hanging drop microscopic examination.

3, results:

the traditional Chinese medicine powder is used for treating 83 infected eperythrozoon sick pigs, 51 pigs are cured, 19 pigs are improved, and 13 pigs are ineffective, the cure rate is 61.4%, and the effective rate is 84.3%. 80 infected eperythrozoon affected pigs are treated by the amitraz, 39 cases are cured, 21 cases are improved, 20 cases are ineffective, the cure rate is 48.8%, and the effective rate is 75.0%. The results show that the cure rate and the cure rate of the traditional Chinese medicine group for treating the eperythrozoonosis of the pigs are higher than those of the amitraz group.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The traditional Chinese medicine composition for preventing and treating the eperythrozoonosis of the pigs is characterized by being prepared from the following raw materials in parts by weight: 3 parts of croton and 2 parts of liquorice.

2. The preparation method of the traditional Chinese medicine composition for preventing and treating pig eperythrozoonosis according to claim 1, which is characterized in that croton and liquorice are crushed, sieved by a third sieve and mixed uniformly according to a ratio to prepare powder.

3. The application of the traditional Chinese medicine composition for preventing and treating the swine eperythrozoonosis according to claim 1 in preparing a medicine for preventing and treating the swine eperythrozoonosis.

4. The use of claim 3, wherein the amount of the composition administered is 2mg per kg body weight per day for 3 consecutive days.