CN107151625B - Liquid microorganism capturing device - Google Patents
Liquid microorganism capturing device Download PDFInfo
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- CN107151625B CN107151625B CN201710434643.0A CN201710434643A CN107151625B CN 107151625 B CN107151625 B CN 107151625B CN 201710434643 A CN201710434643 A CN 201710434643A CN 107151625 B CN107151625 B CN 107151625B
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- filter
- liquid
- filter membrane
- capture device
- membrane
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
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- General Health & Medical Sciences (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a liquid microorganism capturing device, which comprises a filter, wherein a filter membrane is arranged in the filter, a liquid inlet and a liquid outlet are arranged on the filter, a proper distance is arranged between the upper wall in the filter and the filter membrane, and the proper distance is smaller than or equal to the distance from the water dripping direction formed by absorption and convergence on the upper wall of the filter to the filter membrane just in contact with enough microorganisms to grow into colonies. The invention sets a proper interval between the filter inner upper wall and the filter membrane. The proper spacing is to design the filter into a fully-closed flat structure, and the filter is not influenced by exogenous air in the filtering process. Meanwhile, the space with proper interval flat can effectively avoid liquid from scattering or balling under microgravity. After the filtration is completed, a culture medium can be added into the capturer, the absorption pad is used for absorbing the culture medium and providing nutrition so that microorganisms captured on the filter membrane grow and form colonies, the upper surface of the filter is smooth and transparent, the results can be observed and counted after the culture, and the filter can also be used for filtering liquid to remove microorganisms or impurities.
Description
Technical Field
The invention mainly relates to a liquid microorganism capturing device, in particular to a device which has small volume and is suitable for microorganism capturing under the microgravity environment without power and cleanliness requirements.
Background
The method of membrane filtration is used for capturing microorganisms, and is widely applied to microorganism detection or sterilization filtration of liquid at present. The current membrane filtration device needs to be used in clean or special environment, otherwise, the device may be polluted by microorganisms contained in air, and the liquid collection needs to be concentrated on the surface of the filter membrane under the action of gravity so as to be completely drained. In the microgravity environment, the liquid cannot automatically concentrate on the surface of the filter membrane, so that the liquid cannot be drained, and the liquid which is not drained possibly contains microorganisms to be detected, so that the microorganisms cannot be accurately detected, and in addition, the liquid also can influence the later culture and observation. And the working environment of microgravity is difficult to provide a clean environment for operation, a common filter needs to be used for taking a film and pasting the film onto an agar plate for culture after filtering, and under the non-clean environment of microgravity, the agar plate cannot be prepared and the microorganism detection result can be influenced by the environment.
Disclosure of Invention
The invention provides a device capable of capturing microorganisms in a microgravity unclean environment, which solves the problems existing in the prior art in use.
The technical scheme for solving the existing problems is as follows: the utility model provides a liquid microorganism capture device, includes the filter, is equipped with the filter membrane in the filter, is equipped with inlet, liquid outlet on the filter, as the improvement, filter in be equipped with suitable interval between upper wall and the filter membrane, suitable interval less than or equal to adsorb and assemble the distance that forms water dripping side to just contact to the filter membrane, and enough microorganism growth colony at the filter upper wall.
As a further improvement, the distance between the filter membrane and the filter is 0.5-2mm.
As a further improvement, the liquid inlet of the filter is connected with two or more liquid inlet pipelines; the upper surface of the filter is flat and transparent.
As a further improvement, the bottom of the filter membrane is provided with an absorption pad.
As a further improvement, the utility model also comprises a cap plug arranged at the liquid inlet and the liquid outlet.
As a further improvement, the filter is provided with a positioning and cap plug fixing rod.
As a further improvement, the liquid inlet pipelines are all provided with cap plugs.
As a further improvement, one or more syringes for injecting liquid into the filter or/and sucking liquid from downstream of the filter are also included.
As a further improvement, the syringes are syringes with different volumes.
As a further improvement, a peristaltic pump is also included connected downstream of the liquid outlet.
Compared with the prior art, the invention has the beneficial effects that the proper distance is arranged between the inner upper wall of the filter and the filter membrane, and the proper distance is smaller than or equal to the distance which is formed by adsorbing and converging water dripping parts on the upper wall of the filter and just contacts the filter membrane and is enough for microorganisms to grow into bacterial colonies. The proper spacing is to design the filter into a fully-closed flat structure, and the filter is not influenced by exogenous air in the filtering process. Meanwhile, the space with proper interval flat can effectively avoid liquid from scattering or balling under microgravity. After the filtration is completed, a culture medium can be added into the capturer, the absorption pad is used for absorbing the culture medium and providing nutrition so that microorganisms captured on the filter membrane grow and form colonies, the upper surface of the filter is smooth and transparent, the results can be observed and counted after the culture, and the filter can also be used for filtering liquid to remove microorganisms or impurities.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is a schematic view of the structure of the filter of the present invention.
Fig. 3 is an enlarged schematic view at fig. 2 a.
Fig. 4-6 are each an embodiment of the present invention.
Detailed Description
Referring to fig. 1-6, the invention comprises a filter 1, a filter membrane 2 is arranged in the filter 1, a liquid inlet 11 and a liquid outlet 12 are arranged on the filter 1, a proper distance is arranged between the upper wall 21 in the filter 1 and the filter membrane 2, and the proper distance is smaller than or equal to a distance from a water dripping part formed by adsorbing and converging on the upper wall 21 of the filter 1 to the filter membrane 2 just contacting with the filter membrane 2 and enough microorganisms to grow into bacterial colonies, so that even trace liquid can be effectively filtered from the filter membrane 2, and liquid is prevented from scattering or balling under microgravity. The spacing between the specific filter membrane 2 and the filter 1 is 0.5-2mm, and the spacing can be set to be 0.5mm, 1mm and 1.5mm according to different liquids, and the maximum is not more than 2mm. The bottom of the filter membrane 2 can be provided with an absorption pad 3. And the cap plug 6 is arranged at the liquid inlet 11 and the liquid outlet 12. The filter 1 is provided with a fixed rod 13 for positioning and placing the cap plug 6. The upper surface of the filter 1 is flat and transparent for observation or counting.
To facilitate micromanipulation, one or more syringes 5 may be included to inject fluid into the filter 1 or/and to draw fluid from downstream of the filter 1, the syringes 5 may be configured as one or more volume syringes, the syringes 5 being configured to create pressure or negative pressure within the filter 1 to allow fluid to be filtered under micromanipulation conditions.
The liquid inlet 11 is connected by the injector 5, liquid is filtered and pressed on the upstream of the liquid inlet 11, liquid culture medium is added from the upstream after the filtration is finished, the filtration is continued, the injector 5 is taken down after the filtration is finished again, and the ports of the liquid inlet 11 and the liquid outlet 12 of the filter 1 are plugged by the cap plug 6 for cultivation.
Referring to fig. 4-5, as another embodiment, the liquid inlet 11 of the filter 1 is connected with two or more liquid inlet pipelines 4, and can be used for continuously taking liquid and simultaneously injecting corresponding liquid from the multi-pipe intersection or performing corresponding operation. If more than two liquid inlet pipelines 4 are arranged, the liquid inlet pipelines 4 are provided with cap plugs 6. Two or more liquid inlet pipelines 4 can be connected with the liquid inlet 11 through corresponding multi-way valves. More than two liquid inlet pipelines 4 can be respectively arranged into a first butt joint pipeline 41 which is in butt joint with a container or a sampling needle cylinder, a second pipeline 42 which is in butt joint with a culture medium container or a culture medium needle cylinder and an air filter pipeline 43, and the multi-pipeline arrangement can be convenient for rapid continuous operation. After the operation is completed, the corresponding pipeline can be closed through the corresponding cap stopper 6 on the pipeline, which is equivalent to plugging the liquid inlet 11, and simultaneously, the liquid outlet 12 is closed through the corresponding cap stopper 6.
Referring to fig. 5-6, as another embodiment, peristaltic pump 7, check valve 71 or other tubing may be connected downstream of outlet 12, and recovery vessel tubing 71 may also be connected downstream of peristaltic pump 7. Liquid is pumped and filtered at the downstream of the liquid outlet 12 by a peristaltic pump 7 or other driving devices, liquid culture medium is added from the upstream of the liquid inlet 11 after the filtration is finished, the filtration is continued, and the hoses at the two ends are clamped and sheared for cultivation after the filtration is finished again.
The peristaltic pump 7 or other driving devices are used for carrying out pressure filtration on the liquid at the upstream of the liquid inlet 11, after filtration is finished, liquid culture medium is added from the upstream of the liquid inlet 11 and filtration is continued, and after filtration is finished again, hoses at two ends are clamped and sheared for cultivation.
Claims (10)
1. The utility model provides a liquid microorganism capture device, includes the filter, is equipped with the filter membrane in the filter, is equipped with inlet, liquid outlet on the filter, its characterized in that: the filter is characterized in that a proper distance is arranged between the inner upper wall of the filter and the filter membrane, and the proper distance is smaller than or equal to the distance from the water dripping part formed by the adsorption and convergence on the upper wall of the filter to the filter membrane just in contact with enough microorganisms to form colonies.
2. The microorganism capture device of claim 1, wherein: the distance between the filter membrane and the filter is 0.5-2mm.
3. The microorganism capture device of claim 1 or 2, wherein: the liquid inlet of the filter is connected with two or more liquid inlet pipelines; the upper surface of the filter is smooth and transparent.
4. The microorganism capture device of claim 1, wherein: the bottom of the filter membrane is provided with an absorption pad.
5. The microorganism capture device of claim 1, wherein: the device also comprises a cap plug arranged at the liquid inlet and the liquid outlet.
6. The microorganism capture apparatus of claim 5, wherein: the filter is provided with a positioning and cap plug placing fixing rod.
7. A microorganism capture device according to claim 3, wherein: the liquid inlet pipelines are provided with cap plugs.
8. The microorganism capture device of claim 1, wherein: one or more syringes for injecting fluid into the filter and/or for withdrawing fluid from downstream of the filter are also included.
9. The microorganism capture device of claim 8, wherein: the syringes are syringes with different volumes.
10. The microorganism capture device of claim 1 or 5, wherein: and a peristaltic pump connected with the downstream of the liquid outlet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710434643.0A CN107151625B (en) | 2017-06-09 | 2017-06-09 | Liquid microorganism capturing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710434643.0A CN107151625B (en) | 2017-06-09 | 2017-06-09 | Liquid microorganism capturing device |
Publications (2)
| Publication Number | Publication Date |
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| CN107151625A CN107151625A (en) | 2017-09-12 |
| CN107151625B true CN107151625B (en) | 2023-08-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710434643.0A Active CN107151625B (en) | 2017-06-09 | 2017-06-09 | Liquid microorganism capturing device |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201793570U (en) * | 2010-09-03 | 2011-04-13 | 中国人民解放军军事医学科学院微生物流行病研究所 | Positive/negative pressure facility detection system for biological detection of high efficiency particulate air filter |
| CN102199537A (en) * | 2011-04-13 | 2011-09-28 | 北京理工大学 | Membrane bioreactor used in microgravity environment and simulated microgravity environment |
| FR3017366A3 (en) * | 2014-02-13 | 2015-08-14 | Benoit Epsztein | AEROSTAT WING A PARTICULATE SENSOR OF MATERIALS ABOVE MEGALOPOLS BY DISPERSION-RECOVERY OF MASSES OF WATER DROPLETS AND BEEES AND METHODS |
| CN205313536U (en) * | 2015-12-07 | 2016-06-15 | 浙江泰林生物技术股份有限公司 | Microorganism limit inspection film filtration device |
| CN206121266U (en) * | 2016-08-17 | 2017-04-26 | 浙江泰林生物技术股份有限公司 | Filter head |
| CN207047227U (en) * | 2017-06-09 | 2018-02-27 | 杭州泰林精密仪器有限公司 | A kind of liquid microbe trap setting |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201004836D0 (en) * | 2010-03-23 | 2010-05-05 | Airbus Uk Ltd | Coalescing filter |
-
2017
- 2017-06-09 CN CN201710434643.0A patent/CN107151625B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201793570U (en) * | 2010-09-03 | 2011-04-13 | 中国人民解放军军事医学科学院微生物流行病研究所 | Positive/negative pressure facility detection system for biological detection of high efficiency particulate air filter |
| CN102199537A (en) * | 2011-04-13 | 2011-09-28 | 北京理工大学 | Membrane bioreactor used in microgravity environment and simulated microgravity environment |
| FR3017366A3 (en) * | 2014-02-13 | 2015-08-14 | Benoit Epsztein | AEROSTAT WING A PARTICULATE SENSOR OF MATERIALS ABOVE MEGALOPOLS BY DISPERSION-RECOVERY OF MASSES OF WATER DROPLETS AND BEEES AND METHODS |
| CN205313536U (en) * | 2015-12-07 | 2016-06-15 | 浙江泰林生物技术股份有限公司 | Microorganism limit inspection film filtration device |
| CN206121266U (en) * | 2016-08-17 | 2017-04-26 | 浙江泰林生物技术股份有限公司 | Filter head |
| CN207047227U (en) * | 2017-06-09 | 2018-02-27 | 杭州泰林精密仪器有限公司 | A kind of liquid microbe trap setting |
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| CN107151625A (en) | 2017-09-12 |
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