CN107151625A - A kind of liquid microbe trap setting - Google Patents
A kind of liquid microbe trap setting Download PDFInfo
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- CN107151625A CN107151625A CN201710434643.0A CN201710434643A CN107151625A CN 107151625 A CN107151625 A CN 107151625A CN 201710434643 A CN201710434643 A CN 201710434643A CN 107151625 A CN107151625 A CN 107151625A
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- Prior art keywords
- filter
- trap setting
- microorganism
- filter membrane
- liquid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
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- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of liquid microbe trap setting, including filter, filter membrane is provided with filter, filter is provided with inlet, liquid outlet, appropriate spacing is provided with filter between upper wall and filter membrane, is suitably smaller than being equal to absorption and converges at filter upper wall and form below water droplet that contact grows into the distance of bacterium colony to filter membrane and enough microorganisms just.The present invention will set appropriate spacing between upper wall and filter membrane in filter.Filter is designed as in totally enclosed flat structure, filter process to be influenceed by external source air by the appropriate spacing.It is while the appropriate flat space of spacing is it is possible to prevente effectively from liquid dissipates excessive or balling-up under microgravity.Culture medium can be added after the completion of filtering into catcher, culture medium is absorbed using absorption pad and provides nutrition so that the microorganism captured on filter membrane grows and forms bacterium colony, filter upper surface flat transparent, result can be observed after incubation and counted, can be used for filtered fluid to remove microorganism or impurity.
Description
Technical field
The invention mainly relates to a kind of liquid microbe trap setting, especially a kind of small volume, it is adaptable to unpowered, nothing
The device of microorganism capture is carried out under the microgravity environment of purity requirements.
Background technology
Microorganism is captured using the method for membrane filtration, is widely used in the microorganism detection or degerming mistake of liquid at present
Filter.Current film filter needs to use under clean or specific environment, and the microorganism that otherwise may be contained in air is dirty
Dye, and the collection of liquid needs to concentrate on filter membrane surface under Action of Gravity Field and could be filtered dry completely.And under microgravity environment, liquid
Filter membrane surface can not be concentrated on automatically, therefore can not be filtered dry, and the liquid internal not being filtered dry may contain micro- life of detection in need
Thing, so as to lead to not accurate detection, in addition, liquid can also influence the culture and observation in later stage.And the working environment of microgravity
It is difficult to provide clean environment and is operated, plain filter needs to take film to be attached on agar plate after filtering is cultivated, and
Under the non-clean environment of microgravity, agar plate can not be prepared and microorganism detection result can be influenceed by environment.
The content of the invention
The present invention for solve prior art exist in use the problem of there is provided it is a kind of can microgravity non-clean ring
The device of microorganism capture is carried out under border.
The present invention solve existing issue technical scheme be：A kind of liquid microbe trap setting, including filter, filtering
Filter membrane is provided with device, filter is provided with inlet, liquid outlet, as an improvement, in described filter between upper wall and filter membrane
Provided with appropriate spacing, it is described it is appropriate be smaller than being equal to absorption converge at filter upper wall and form lucky below water droplet contact
To filter membrane and microorganism grows into the distance of bacterium colony enough.
As a further improvement, the spacing between described filter membrane and filter is 0.5-2mm.
As a further improvement, water influent pipeline more than described filter inlet connection two-way or two-way；Above-mentioned
Filter upper surface flat transparent.
As a further improvement, described filter membrane bottom is provided with absorption pad.
As a further improvement, also including the cap stopper for being arranged at inlet and liquid outlet.
As a further improvement, described filter places cap stopper fix bar provided with positioning.
As a further improvement, described water influent pipeline is equipped with cap stopper.
Inhaled as a further improvement, also including one or more fluid injection to filter or/and from filter downstream
Take the syringe of liquid.
As a further improvement, syringe of the described syringe for not co-content.
As a further improvement, also including the peristaltic pump in connection liquid outlet downstream.
Compared with prior art, beneficial effect is between being set suitably between upper wall and filter membrane in filter to the present invention
Away from this, which is suitably smaller than converging at filter upper wall equal to absorption, forms below water droplet contact just to filter membrane and enough
Microorganism grows into the distance of bacterium colony.Filter is designed as in totally enclosed flat structure, filter process by the appropriate spacing
It will not be influenceed by external source air.It is while the appropriate flat space of spacing is it is possible to prevente effectively from liquid dissipates excessive under microgravity
Or balling-up.Culture medium can be added after the completion of filtering into catcher, using absorption pad absorb culture medium and provide nutrition cause filter
The microorganism captured on film grows and forms bacterium colony, filter upper surface flat transparent, result can be observed after incubation and counted,
Filtered fluid is can be used for remove microorganism or impurity.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention.
Fig. 2 is the structural representation of filter of the present invention.
Fig. 3 is the enlarged diagram at Fig. 2 a.
Fig. 4-6 is a kind of case study on implementation of the present invention respectively.
Embodiment
Referring to Fig. 1-6, the present invention includes being provided with filter membrane 2 in filter 1, filter 1, filter 1 provided with inlet 11,
Appropriate spacing is provided with liquid outlet 12, filter 1 between upper wall 21 and filter membrane 2, is suitably smaller than converging at equal to absorption
The upper wall 21 of filter 1 forms the distance that lucky contact below water droplet grows into bacterium colony to filter membrane 2 and enough microorganisms, so may be used
It is effectively ensured and can be also filtered even if micro liquid from filter membrane 2, it is to avoid liquid dissipates under microgravity and overflow or balling-up.Specific filter membrane 2
Spacing between filter 1 is 0.5-2mm, spacing can be set into 0.5mm, 1mm, 1.5mm according to different liquids, most very much not
More than 2mm.The bottom of filter membrane 2 can be provided with absorption pad 3.Also include the cap stopper 6 for being arranged at inlet 11 and liquid outlet 12.Filter 1
The fix bar 13 of cap stopper 6 is placed provided with positioning.The upper surface flat transparent of filter 1, for observing or counting.
For the ease of micromanipulation, in addition to one or more fluid injection is to filter 1 or/and under filter 1
The syringe 5 of liquid draw is swum, syringe 5 may be configured as the syringe of one or more volumes, and syringe 5 is mainly used in filtering
Pressure or negative pressure are produced in device 1, liquid is also filtered under micro situation.
Inlet 11 is connected using syringe 5, in the upstream press filtration liquid of inlet 11, is completed after filtering from upstream annex solution
Body culture medium simultaneously continue filtering, filtering again after the completion of syringe 5 is removed, with cap stopper 6 clog filter 1 inlet 11 and
Cultivated the port of liquid outlet 12.
Referring to Fig. 4-5, as another case study on implementation, the inlet 11 of filter 1 connects feed liquor more than two-way or two-way
Pipeline 4, takes liquid and injects corresponding liquid from multitube crossing simultaneously or operated accordingly available for continuous.If setting two
Water influent pipeline 4 more than road, then water influent pipeline 4 be equipped with cap stopper 6.Water influent pipeline 4 more than two-way or two-way can be by corresponding
Multiple-way valve connection inlet 11.Water influent pipeline 4 more than two-way can be respectively set to container or sample the first couple of syringe
Adapter road 41, the second pipeline 42, the air cleaner pipeline 43 that are docked with medium container or culture medium syringe, multi-pipeline are set
Quick continuous operation can be easy to.After the completion of operation, corresponding pipeline can be closed by the cap stopper 6 in respective line, be equal to plug
Inlet 11 is firmly closed, while closing liquid outlet 12 by clogging cap stopper 6 accordingly.
Referring to Fig. 5-6, as another case study on implementation, the attachable peristaltic pump 7 in the downstream of liquid outlet 12, check valve 71 or its
His pipeline, the downstream of peristaltic pump 7 can also connect returnable pipeline 71.Using peristaltic pump 7 or other drive devices in liquid outlet
12 downstream suction filtration liquid, complete filtering after from the upstream adding liquid culture medium of inlet 11 and continue filtering, filtering complete again
Two ends flexible pipe is clamped afterwards and cultivated after cutting.
Press filtration liquid is carried out in the upstream of inlet 11 using peristaltic pump 7 or other drive devices, is completed after filtering from feed liquor
Mouthful 11 upstream adding liquid culture mediums simultaneously continue filtering, clamp two ends flexible pipe after the completion of filtering again and are trained after cutting
Support.
Claims (10)
1. a kind of liquid microbe trap setting, including filter, be provided with filter membrane in filter, filter provided with inlet,
Liquid outlet, it is characterised in that：Appropriate spacing is provided with described filter between upper wall and filter membrane, described is suitably smaller than
Filter upper wall, which is converged at, equal to absorption forms that contact is to filter membrane just below water droplet and enough microorganisms grow into bacterium colony
Distance.
2. microorganism trap setting as claimed in claim 1, it is characterised in that：Spacing between described filter membrane and filter
For 0.5-2mm.
3. microorganism trap setting as claimed in claim 1 or 2, it is characterised in that：Described filter inlet connection two
Water influent pipeline more than road or two-way；Described filter upper surface flat transparent.
4. microorganism trap setting as claimed in claim 1, it is characterised in that：Described filter membrane bottom is provided with absorption pad.
5. microorganism trap setting as claimed in claim 1, it is characterised in that：Also include being arranged at inlet and liquid outlet
Cap stopper.
6. microorganism trap setting as claimed in claim 5, it is characterised in that：Described filter places cap provided with positioning
Fill in fix bar.
7. microorganism trap setting as claimed in claim 3, it is characterised in that：Described water influent pipeline is equipped with cap stopper.
8. microorganism trap setting as claimed in claim 1, it is characterised in that：Also given including one or more fluid injection
Filter or/and the syringe from filter downstream liquid draw.
9. microorganism trap setting as claimed in claim 8, it is characterised in that：Described syringe is the injection of not co-content
Device.
10. the microorganism trap setting as described in claim 1 or 5, it is characterised in that：Also include the compacted of connection liquid outlet downstream
Dynamic pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710434643.0A CN107151625B (en) | 2017-06-09 | 2017-06-09 | Liquid microorganism capturing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710434643.0A CN107151625B (en) | 2017-06-09 | 2017-06-09 | Liquid microorganism capturing device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107151625A true CN107151625A (en) | 2017-09-12 |
| CN107151625B CN107151625B (en) | 2023-08-22 |
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ID=59796197
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710434643.0A Active CN107151625B (en) | 2017-06-09 | 2017-06-09 | Liquid microorganism capturing device |
Country Status (1)
| Country | Link |
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| CN (1) | CN107151625B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201793570U (en) * | 2010-09-03 | 2011-04-13 | 中国人民解放军军事医学科学院微生物流行病研究所 | Positive/negative pressure facility detection system for biological detection of high efficiency particulate air filter |
| CN102199537A (en) * | 2011-04-13 | 2011-09-28 | 北京理工大学 | Membrane bioreactor used in microgravity environment and simulated microgravity environment |
| US20120312022A1 (en) * | 2010-03-23 | 2012-12-13 | Airbus Operations Limited | Coalescing filter |
| FR3017366A3 (en) * | 2014-02-13 | 2015-08-14 | Benoit Epsztein | AEROSTAT WING A PARTICULATE SENSOR OF MATERIALS ABOVE MEGALOPOLS BY DISPERSION-RECOVERY OF MASSES OF WATER DROPLETS AND BEEES AND METHODS |
| CN205313536U (en) * | 2015-12-07 | 2016-06-15 | 浙江泰林生物技术股份有限公司 | Microorganism limit inspection film filtration device |
| CN206121266U (en) * | 2016-08-17 | 2017-04-26 | 浙江泰林生物技术股份有限公司 | Filter head |
| CN207047227U (en) * | 2017-06-09 | 2018-02-27 | 杭州泰林精密仪器有限公司 | A kind of liquid microbe trap setting |
-
2017
- 2017-06-09 CN CN201710434643.0A patent/CN107151625B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120312022A1 (en) * | 2010-03-23 | 2012-12-13 | Airbus Operations Limited | Coalescing filter |
| CN201793570U (en) * | 2010-09-03 | 2011-04-13 | 中国人民解放军军事医学科学院微生物流行病研究所 | Positive/negative pressure facility detection system for biological detection of high efficiency particulate air filter |
| CN102199537A (en) * | 2011-04-13 | 2011-09-28 | 北京理工大学 | Membrane bioreactor used in microgravity environment and simulated microgravity environment |
| FR3017366A3 (en) * | 2014-02-13 | 2015-08-14 | Benoit Epsztein | AEROSTAT WING A PARTICULATE SENSOR OF MATERIALS ABOVE MEGALOPOLS BY DISPERSION-RECOVERY OF MASSES OF WATER DROPLETS AND BEEES AND METHODS |
| CN205313536U (en) * | 2015-12-07 | 2016-06-15 | 浙江泰林生物技术股份有限公司 | Microorganism limit inspection film filtration device |
| CN206121266U (en) * | 2016-08-17 | 2017-04-26 | 浙江泰林生物技术股份有限公司 | Filter head |
| CN207047227U (en) * | 2017-06-09 | 2018-02-27 | 杭州泰林精密仪器有限公司 | A kind of liquid microbe trap setting |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107151625B (en) | 2023-08-22 |
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