CN107118958A - Nucleic acid purifier - Google Patents
Nucleic acid purifier Download PDFInfo
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- CN107118958A CN107118958A CN201710569179.6A CN201710569179A CN107118958A CN 107118958 A CN107118958 A CN 107118958A CN 201710569179 A CN201710569179 A CN 201710569179A CN 107118958 A CN107118958 A CN 107118958A
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 50
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 50
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 50
- 238000003756 stirring Methods 0.000 claims abstract description 170
- 230000007246 mechanism Effects 0.000 claims abstract description 111
- 230000003028 elevating effect Effects 0.000 claims abstract description 68
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000013519 translation Methods 0.000 claims description 32
- 229910000838 Al alloy Inorganic materials 0.000 claims description 3
- 239000000956 alloy Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 241000237983 Trochidae Species 0.000 claims 1
- 238000001821 nucleic acid purification Methods 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 14
- 238000012423 maintenance Methods 0.000 abstract description 4
- 238000010276 construction Methods 0.000 abstract description 2
- 230000009471 action Effects 0.000 description 30
- 230000014616 translation Effects 0.000 description 25
- 239000011324 bead Substances 0.000 description 17
- 238000000034 method Methods 0.000 description 16
- 230000033001 locomotion Effects 0.000 description 15
- 230000008569 process Effects 0.000 description 9
- 238000010828 elution Methods 0.000 description 7
- 230000005540 biological transmission Effects 0.000 description 6
- 238000005452 bending Methods 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 238000005096 rolling process Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 238000005336 cracking Methods 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 230000011664 signaling Effects 0.000 description 3
- 230000001360 synchronised effect Effects 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 238000005299 abrasion Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000008450 motivation Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of nucleic acid purifier, including mechanical part, control system, operating unit, shell.Mechanical part includes：Stirring set drive mechanism, bar magnet elevating mechanism, bracket lifting device, bracket translating device, stirring stock, bar magnet frame, pedestal, workbench.Present invention stirring set drive mechanism is slider-crank mechanism, and stirring motor continuously rotates in the same direction without rotating, drives the stirring stock being fixed on stirring set sliding block to move up and down and carry out sample stirring along stirring set guide rail by stirring set sliding block.Bracket elevating mechanism can drive bracket system, then drive bar magnet and stirring set synchronization lifting.The nucleic acid purifier of the present invention can realize that high-frequency large amplitude stirs sample, can effectively improve nucleic acid purification effect, and simple in construction, reliable operation, small power consumption, vibrate that small, noise is small, cost is low, easy to maintenance.
Description
Technical field
The present invention relates to bio-instruments field, more particularly to the nucleic acid purification automatics based on nanometer magnetic bead.
Background technology
Nucleic acid（DNA or RNA）It is widely present in animal and plant body.In clinical diagnosis and treatment, or Forensic Identification, or base
Because examination and restructuring etc. are in work, people are needed nucleic acid from sample（Blood, saliva or its hetero-organization）In extract, extract
The process of nucleic acid is also referred to as nucleic acid purification.The effect quality of nucleic acid purification directly affects the process and result of research and diagnosis, because
This nucleic acid purification techniques is an important technology in biotechnology.
A kind of nucleic acid purification method common at present is bar magnet method, and bar magnet method is to adsorb nanometer magnetic bead, nanometer using bar magnet
Magnetic bead adsorbs nucleic acid, and nucleic acid is separated from sample.The operation principle of bar magnet method is as shown in figure 1, bar magnet 20, stirring are covered
21st, deep-well plates 22 are sequentially arranged from top to bottom；Wherein, there is one group of deep hole in deep-well plates 22, this group of deep hole includes cracking fluid apertures
221st, magnetic bead hole 222, washing fluid apertures 223（Multiple washing fluid apertures can be set）, elution fluid apertures 224, corresponding reagent is deposited respectively.
Nucleic acid purification flow is comprised the concrete steps that：Sample is instilled to and nucleic acid is cracked out in lysate -- collecting magnetic bead to cracking fluid apertures -- magnetic
Pearl is combined with nucleic acid -- magnetic bead is collected to washing hole（Can repeatedly it wash）-- collecting magnetic bead to elution hole -- reclaims magnetic bead after elution,
Final nucleic acid is stayed in eluent.Automation mechanized operation is realized for the ease of above-mentioned flow, it is necessary to which nucleic acid purifier is realized such as Fig. 1 institutes
The elemental motion shown, i.e.,：（1）Bar magnet 20 moves up and down a,（2）That stirs set 21 moves up and down b and stirring action c,（3）Magnetic
E is moved up and down after both zoariums of the insertion stirring of rod 20 set 21,（4）Translation after both zoariums of the insertion stirring of bar magnet 20 set 21 is moved
Make d.
Chinese invention patent CN103897987B discloses a kind of nucleic acid automatic extracting device based on nanometer magnetic bead, core
Main three mechanisms by pedestal, on pedestal of sour extraction element are constituted.Three mechanisms are respectively：Bar magnet lift
Structure, stirring set drive mechanism and deep-well plates translation mechanism.Three mechanisms are, by stepper motor, leading screw and nut mechanism, to play support
Constituted with the slide bar of guide effect.Fig. 1, a are shown in the action that above three mechanism is realized respectively：Bar magnet elevating mechanism drives bar magnet 20
Move up and down, effect is to insert or take out stirring set 21 by bar magnet 20；b：Stirring set drive mechanism driving stirring set 21 is at a slow speed
Move up and down；c：Stirring set drive mechanism driving stirring set 21 is quick stirs up and down；d：Deep-well plates translation mechanism drives deep-well plates
22 or so translations（Equivalent to bar magnet 20 and the left and right translation motion after stirring 21 zoariums of set）；e：Bar magnet elevating mechanism and stirring cover
Drive mechanism coordinates, synchronization action, realizes bar magnet 20 and moving up and down after stirring 21 zoariums of set.
The shortcoming of above-mentioned nucleic acid-extracting apparatus is：
Firstth, stirring set drive mechanism from stepper motor by screw mandrel and feed screw nut driving stirring set 21 carried out on slide bar toward
Multiple motion, realizes moving up and down at a slow speed and quick stirring action for stirring set 21.Wherein, the stirring action of stirring set 21 is high frequency
Rate is moved back and forth, stepper motor must high-frequency rotating could realize.
When stirring set is stirred, the energy of motor is transformed by the power supply of system, main dynamic including rotor
Can be with the electromagnetic energy stored in armature.According to Newton's law, the energy that motor is rotated forward must be disappeared, and motor could be inverted；
Conversely, the energy of motor reversal must disappear, motor could be rotated forward.In high frequency rotating, motor frequently must be braked or subtracted
Speed could realize commutation.Most of energy of motor is not intended to stirring, does not form useful work, but by frequent braking wave
Take, thus inefficiency.
According to law of conservation of energy, energy can neither be produced without foundation, will not also be disappeared without foundation, and it can only turn from a kind of form
Another form is turned to, or other objects are transferred to from an object.Herein, the energy turn that motor is wasted due to rotating
Turn to heat energy and mechanical energy and produce negative interaction.Heat energy causes motor and whole system to be raised including circuit system temperature.Machinery
System vibration and noise can then be caused.
In order to reduce vibration and noise, existing instrument has to use heavier instrument base, to reduce resonant frequency, institute
It is heavier with instrument, cause the difficulty and added cost of transport.
Restricted by stepper motor dynamic characteristic, stepper motor can lose under band load during high-frequency significantly rotating
Step, the energy loss that now motor positive and inverse is brought is more serious.So when stirring frequency is higher, it is impossible to while significantly stirring
Mix.If realizing that high-frequency is significantly stirred it is necessary to select more powerful motor or more expensive motor and parts, than
Such as high-power stepper motor, servomotor, ball screw.These parts with respect to low-power stepper motor and common screw mandrel, into
This can increase decades of times.So the stirring set drive mechanism of prior art is difficult to high-frequency significantly stirring action.Its
As a result, when sample is high viscosity liquid or sample size is larger or can not normal work during high sample impurity content.Because high
Viscosity or the larger or high impurity content sample of amount need the stirring of high-frequency large amplitude, could clean up nucleic acid.It is no
Then, stirring amplitude does not reach requirement, can cause nucleic acid purification effect difference or even extract failure.
In above-mentioned stirring set drive mechanism, the transmission mechanism that stepper motor is connected uses screw mandrel and feed screw nut, also has
Instrument use synchronous belt drive mechanism.Both mechanisms noise in high-frequency back and forth movement is very big.And screw mandrel and silk
Stem nut is high-precision drive disk assembly, under the reciprocating action of long term frequent, easy wear out failure, thus maintenance cost compared with
It is high.Timing belt can fatigue, fracture because of long term frequent reciprocating motion.
So, above-mentioned stirring covers drive mechanism, it is impossible to be used in the sample for needing high frequency significantly to stir, and energy consumption is big, effect
Rate is low, vibration is big, noise is big, cost is high, instrument weight, and maintenance rate is high.
Secondth, during nucleic acid purification, an action is performed a plurality of times, bar magnet and the stirring latter lifting of engaging body, i.e.,
Fig. 1 e actions.Above-mentioned action coordinates by bar magnet elevating mechanism and stirring set drive mechanism in the prior art, synchronous up and down to move
It is dynamic to complete.If Liang Ge mechanisms are slightly asynchronous, the magnetic bead with nucleic acid can be lost in outside deep hole, cause nucleic acid extraction to interrupt
Or failure.So this manner of execution has risk, cause instrument work unreliable.
3rd, in the prior art, stirring stock, bar magnet frame, the hole on the clamping device of deep-well plates and elongated round bar shape
Slide bar coordinates, and stirring set, bar magnet, deep-well plates are moved back and forth on slide bar.Slide bar is cylindrical larger with hole frictional force.And erect
The long slide bar only end set is fixed, and is easily bent.Even if slide bar has the minimum bending that naked eyes can not be recognized, it can also fill clamping
The slip put has some setbacks, or even can not act.Therefore, the support for not being with slide bar and guidance method.
The content of the invention
For the shortcoming or deficiency of above-mentioned prior art, the present invention provides a kind of nucleic acid purifier, and it stirs set driving machine
Structure is slider-crank mechanism, and motor continuously same direction can rotate, it is not necessary to rotating, even if most being passed through using market is most common
The low-power stepper motor of Ji can just realize stirring movement, it is to avoid what stepper motor rotating was brought in the prior art one is
Row problem.
The technical scheme is that,
A kind of nucleic acid purifier, including mechanical part, control system, operating unit, shell, mechanical part are located at nucleic acid purifier
Bottom, control system and operating unit are located at instrument top, and shell is wrapped in instrument outermost, and mechanical part includes stirring set and driven
Motivation structure, stirring stock, bar magnet elevating mechanism, bar magnet frame, support or supporting plate, pedestal, workbench, translation device for operation table or
Support translating device, stirring stock is installed in stirring set drive mechanism, and bar magnet frame is installed on bar magnet elevating mechanism, stirring set
Drive mechanism is installed in support or supporting plate with bar magnet elevating mechanism, and support or supporting plate are installed on pedestal with workbench,
Workbench is arranged at below bar magnet frame and stirring stock；Characterized in that, stirring set drive mechanism include stirring motor, crank,
Connecting rod, stirring set sliding block, stirring set guide rail, stirring motor axle are fixedly connected with crank, and crank is rotatably connected with connecting rod one end,
The connecting rod other end is rotatably connected with stirring set sliding block, and stirring stock is fixed on stirring set sliding block, stirring set sliding block and stirring
Set guide rail is slidably connected.
Nucleic acid purifier also includes bracket lifting device, and bracket lifting device includes bracket lifting motor, intermediate support, support
Frame elevating screw, bracket elevating screw nut, bracket lifting slider, bracket riser guide, bracket lifting motor are installed on centre
On support, bracket elevating screw is connected with bracket lifting motor axle, and bracket elevating screw nut sleeve is loaded on bracket elevating screw,
Bracket elevating screw nut is connected with supporting plate, and bracket lifting slider is installed on the supporting plate, during bracket riser guide is fixed on
Between on support, bracket lifting slider is slidably connected with bracket riser guide.
The bracket translating device of the present invention includes bracket translation motor, bracket translation screw mandrel, bracket translation feed screw nut, support
Frame translator slider, bracket translating rails, bracket translation motor are installed on pedestal, bracket translation screw mandrel and bracket translation motor axle
Connection, bracket translation feed screw nut is set on bracket translation screw mandrel, and bracket translation feed screw nut is connected with bracket lifting device,
Bracket translator slider is arranged on bracket lifting device, and bracket translating rails are arranged on pedestal, bracket translator slider and bracket
Translating rails are slidably connected.
Bar magnet elevating mechanism includes bar magnet lifting motor, bar magnet elevating screw, bar magnet elevating screw nut, bar magnet lifting cunning
Block, bar magnet riser guide, bar magnet lifting motor axle are connected with bar magnet elevating screw, and bar magnet elevating screw nut sleeve is loaded on bar magnet liter
Drop on screw mandrel, bar magnet elevating screw nut is connected with bar magnet frame, and bar magnet lifting slider is fixed on bar magnet frame, bar magnet lifting slider
It is slidably connected with the bar magnet riser guide.
Stirring set drive mechanism also includes catch, position sensor, bearing, and catch is fixed on crank, position sensor
It is fixed on beside crank, catch is thin plate, and its thickness direction is by the groove on position sensor, and bearing is installed on the hole of connecting rod
It is interior.
Catch is that right angle is fan-shaped.
Stirring set drive mechanism, bar magnet elevating mechanism, bracket translating device, the sliding block in bracket lifting device and guide rail machine
Structure is ball guiding rail mechanism.
Crank, the connecting rod of stirring set drive mechanism are aluminum alloy material.
Crank is less than 1 with length of connecting rod ratio:1, stirring motor frequency is 0 ~ 20Hz.
It is preferred that, crank is 1 with length of connecting rod ratio：4~1：6, stirring motor frequency is 2 ~ 8Hz.
Compared with prior art, the advantage of the invention is that：
The nucleic acid purifier of the present invention, stirring set drive mechanism uses slider-crank mechanism instead.Stirring motor only need to be to same side
To continuous rotation, without rotating, lifting and the stirring action of stirring set can be achieved with.Capacity usage ratio is high, and system temperature rise is low,
Vibration and noise are small, the noise that screw mandrel, timing belt are sent when also completely eliminating stirring.
The instrument vibration of the present invention is small, it is not necessary to aggravate base weight, so the instrument of the present invention is compared with traditional instrument weight
Mitigate more than 1/2, be easy to carry.
The slider-crank mechanism of the present invention can drive stirring set to realize high-frequency significantly stirring action, when sample is height
When viscosity or high impurity liquid, electric machine frequency, increase stirring amplitude are improved（Increase crank length）, it just can strengthen stirring dynamics,
Ensure the nucleic acid purification quality of special sample.
The instrument capacity usage ratio of the present invention is high, only need to using conventional low-power machine, crank block transmission mechanism and
Small-power power, cost of parts is relatively low；Slider-crank mechanism is than screw mandrel or synchronous belt mechanism, and structure is simpler, more durable in use.
In the slider-crank mechanism of the present invention, two endoporus of connecting rod are equipped with bearing.And the movement of stirring set is straight by ball
Line guide rail mechanism is as support and is oriented to, therefore the mutual rotation in mechanism between part or all much smoother, the part of mutual slip
Abrasion is small, and instrument service life is long.
Especially when high-frequency is significantly stirred, above advantage is more prominent.
Stirring action, through whole nucleic acid purification process, is that maximum link is influenceed on purification effect.The present invention is to stirring
The improvement of set drive mechanism is mixed, the performance for raising nucleic acid purifier plays key effect.
(2) the present invention is specially provided with bracket lifting device.The magnetic being arranged on bracket system is driven with bracket lifting device
Rod frame and stirring stock are lifted simultaneously, it is ensured that bar magnet and stirring set Complete Synchronization lifting.Avoid Liang Ge mechanisms and coordinate driving
Under, the risk that caused magnetic bead drops when both are asynchronous substantially enhances the reliability of instrument work.
(3) bracket translating device of the invention, instead of the table translation dress being arranged in the prior art below workbench
Put.Stirring rod on bracket translating device driving bracket system is translated together with bar magnet, reliable operation.And the power of whole instrument
Mechanism is respectively positioned on pedestal rear portion, instrument using safer, be laid out it is more compact, it is attractive in appearance.
The stirring of the present invention is covered in drive mechanism, bar magnet elevating mechanism, bracket lifting device, bracket translating device,
The support as moving parts and guiding mechanism using ball guiding rail mechanism.Force of rolling friction between guide rail and sliding block is more
It is small, make the movement of bar magnet, stirring set more smooth, it is more stable, work more reliable.
The nucleic acid purifier that the present invention is provided, is improved stirring set drive mechanism, can be carried out using inexpensive part
High-frequency is significantly stirred.Capacity usage ratio is high, vibrates small small, noise, light-weight equipment, simple in construction, easy to maintenance, instrument
Cost is low, service life is long, and it is more reliable to work.
Brief description of the drawings
Fig. 1 is nucleic acid purifier elemental motion schematic diagram；
Fig. 2 is nucleic acid purifier outline drawing of the present invention；
Fig. 3 is nucleic acid purifier mechanical part structure chart of the present invention；
Fig. 4 is nucleic acid purifier mechanical part top view of the present invention；
Fig. 5 is nucleic acid purifier mechanical part right view of the present invention；
Fig. 6 is nucleic acid purifier stirring set drive mechanism stereogram of the present invention；
Fig. 7 is nucleic acid purifier stirring set drive mechanism left view of the present invention；
Fig. 8 is nucleic acid purifier stirring set drive mechanism rearview of the present invention；
Fig. 9 is nucleic acid purifier bracket system stereogram of the present invention.
Embodiment
The technique effect of the design of the present invention, concrete structure and generation is described further below with reference to accompanying drawing, with
It is fully understood from the purpose of the present invention, feature and effect.
As shown in Fig. 2 nucleic acid purifier is made up of mechanical part 1, control system 2, operating unit 3, shell 4.Machinery Ministry
1 is divided to be located at nucleic acid purifier bottom, control system 2 and operating unit 3 are located at top, and shell 4 is wrapped in instrument outermost.Machinery
Part 1 is the core component of nucleic acid purifier.
The nucleic acid purifier of the present invention, the lifting of stirring set 21 and stirring action are completed by stirring set drive mechanism 5.Bar magnet
20 lifting is completed by bar magnet elevating mechanism 6.Stirring set drive mechanism 5 is arranged in supporting plate 12 with bar magnet elevating mechanism 6, group
Into bracket system 9.Bar magnet 20 and lifting and translation after stirring 21 zoariums of set, are translated by bracket lifting device 8 and bracket respectively
The driving bracket system 9 of device 7 is completed.Above mechanism is all disposed within pedestal 13 with placing the workbench 14 of deep-well plates 22.
As shown in Fig. 3 and Fig. 9, bar magnet elevating mechanism 6 is by the bar magnet lifting motor 61 installed in the front upper of supporting plate 12
Driving, bar magnet lifting motor 61 is fixed in supporting plate 12 by bar magnet lifting motor installing plate 65.The axle of bar magnet lifting motor 61
The bar magnet elevating screw 62 of connection thereunder is driven to rotate（Or bar magnet elevating screw 62 and the axle of bar magnet lifting motor 61 are one
Body）, bar magnet elevating screw nut 63 moves up and down under the driving of bar magnet elevating screw 62.Bar magnet frame 11 and bar magnet elevating screw
Nut 63 is fixedly connected, and drives bar magnet frame 11 to move up and down by bar magnet elevating screw nut 63.Bar magnet frame 11 is lifted with bar magnet and slided
Block 64 is fixedly connected, and bar magnet lifting slider 64 is slidably connected with the stirring set guide rail 55 in supporting plate 12, so bar magnet
Frame 11 is moved up and down along stirring set guide rail 55, i.e., a actions of the bar magnet frame 11 shown in Fig. 9, and the effect of a actions is to insert bar magnet 20
Enter and take out stirring set 21.The thin slice 66 on bar magnet frame 11 is fixed on, by being installed on bar magnet elevating screw in moving process
During the position sensor 57 of 62 sides, the position signalling of bar magnet elevating mechanism 6 is passed into control system 2, control system 2 is then
Bar magnet lifting motor 61 is controlled, the control that displacement is lifted to bar magnet 20 is realized.
The leading screw and nut mechanism that bar magnet elevating screw 62 is constituted with bar magnet elevating screw nut 63, can be lead screw
Other transmission mechanisms such as nut body, ball-screw nut mechanism, timing belt reciprocating mechanism, pinion and rack.
As shown in Fig. 6, Fig. 7, Fig. 8, the power source of stirring set drive mechanism 5 is stirring motor 51.Stirring motor 51 is installed
In the lower backside of supporting plate 12, the axle of stirring motor 51 is passed through in the middle of the hole of supporting plate 12, and crank 52 is arranged on stirring motor
On 51 axles, crank 52 is located at installing plate front, and the other end of crank 52 passes through hinge, the other end of connecting rod 53 with the one end of connecting rod 53
Pass through hinge with stirring stock 10.Rolling bearing 58, rolling bearing 58 are respectively mounted in the hole coordinated on connecting rod 53 with bearing pin
For supporting bearing pin, and reduce the abrasion of bearing pin.Stirring stock 10 with stirring set sliding block 54 be fixedly connected, stirring set sliding block 54 and
Stirring set guide rail 55 is slidably connected, so stirring motor 51 covers guide rail by slider-crank mechanism driving stirring stock 10 along stirring
55 lift and stirring action, i.e., b and the c action of the stirring set 21 shown in Fig. 6.The effect of b and c actions is that stirring set 21 enters
The deep holes of deep-well plates 22, stirring magnetic bead and liquid and exit deep hole.Cracking and wash phase in nucleic acid purification, stirring motor
51 360 degree of continuous rotations of axle, stir the length between the amplitude of stock 10, two axis holes of the crank 52 equal to twice.In core
The elution stage of acid purifying, because effluent volume is smaller, stirring stock 10 is less than in the amplitude of elution stage to be cracked and cleaning rank
Section, so stirring motor 51 need to the rotating in the range of certain angle in elution stage.Control system 2 is positive and negative to stirring motor 51
The control signal turned, comes from the catch 56 on crank 52, and the light with the lower section of crank 52 of the cooperating of catch 56
Electric position sensor 57.The positional information that stirring motor 51 is rotated is gathered for the ease of photoelectrical position sensor 57, catch 56 is excellent
Elect as right angle sector.The time of elution stage accounts for 1/10 or so of whole nucleic acid purification time, so the motor in this stage
Noise effect very little of the rotating to nucleic acid purifier.
Crank 52, the connecting rod 53 of stirring set drive mechanism 5 are aluminum alloy material, can effectively mitigate the weight of moving component,
To reduce the inertia force of stirring set 21.Crank 52 is taken less than 1 with the lenth ratio of connecting rod 53：1, it can increase connecting rod because connecting rod 53 is long
53 mass, the space for taking mechanism is larger, and connecting rod 53 is too short, causes the lateral pressure increase of stirring set sliding block 54, it is advantageous to
Crank 52 is 1 with the lenth ratio of connecting rod 53:4~1:6.The stirring frequency selection of stirring motor 51 is 0 ~ 20Hz.But according to various
The result of the test of sample, it is believed that stirring frequency can reach optimal mixing effect in the range of 2 ~ 8Hz, it is pure to save nucleic acid again
The change time.
As shown in figure 9, stirring set drive mechanism 5 is installed in supporting plate 12 with bar magnet elevating mechanism 6, stirring set driving
Mechanism 5 is located at the lower section of bar magnet elevating mechanism 6, and stirring stock 10 is located at the lower section of bar magnet frame 11, bar magnet frame 11 and stirring stock 10
The surface of deep-well plates 22 on workbench 14., will for safety and attractive in appearance, the shutter 16 in supporting plate 12
Positioned at the stirring stock 10 and bar magnet frame 11 that pedestal 13 is anterior, separated with rear portion miscellaneous part.Above-mentioned bar magnet elevating mechanism 6, magnetic
Rod frame 11, stirring set drive mechanism 5, stirring stock 10, supporting plate 12, shutter 16 collectively constitute bracket system 9.
As shown in Fig. 3, Fig. 4, Fig. 5, bracket lifting device 8 by bracket lifting motor 81, bracket lifting motor installing plate 82,
Bracket elevating screw 83, bracket elevating screw nut 84, intermediate support 85, supporting plate actuator 86, bracket lifting slider 87, support
Frame riser guide 88, bending thin slice 89 are constituted.Bracket lifting motor 81 is installed on centre by bracket lifting motor installing plate 82
The top of support 85.Bracket elevating screw 83 is fixedly connected with the axle of bracket lifting motor 81（Or bracket elevating screw 83 and bracket liter
The drop axle of motor 81 is integrated）.Bracket elevating screw nut 84 is bolted with supporting plate actuator 86 to be integrated.Such as Fig. 5
Shown, supporting plate actuator 86 is fastenedly connected after being fitted sideways with the supporting plate 12 in bracket system 9.Bracket lifting motor 81 leads to
Crossing bracket elevating screw 83 drives bracket elevating screw nut 84 to move up and down, and bracket elevating screw nut 84 then passes through support
Plate 12 drives bracket system 9 to move up and down.In the positive mounting bracket lifting slider 87 of the supporting plate 12 of bracket system 9, bracket liter
Drop sliding block 87 is slidably connected with the formation above-below direction of bracket riser guide 88 being fastened on intermediate support 85, makes bracket system 9
It can stablize and swimmingly move up and down, in the lifting process of bracket system 9, bar magnet 20 covers the state of 21 bottoms for insertion stirring,
Bar magnet 20 is moved up and down with the zoarium of stirring set 21, i.e., magnetic bead is suctioned out and be put into deep-well plates by e actions as shown in Figure 3, e actions
In 22 deep hole.The bending thin slice 89 on supporting plate actuator 86 is fixed on, by being fixed in the photopotential of intermediate support 85
When putting sensor 57, position signalling is passed into control system 2, control system 2 then controls bracket lifting motor 81.The present invention
The bracket lifting device 8 set up, realizes bar magnet 20 and stirring set 21 is fit（21 bottoms of the insertion stirring set of bar magnet 20）Afterwards together
Move up and down, driven by bracket lifting motor 81 is independent, control is easy in displacement, and the process safety of mobile magnetic bead is reliable.
The leading screw and nut mechanism that bracket elevating screw 83 is constituted with bracket elevating screw nut 84, can be lead screw spiral shell
Other transmission mechanisms such as parent agency, ball-screw nut mechanism, timing belt reciprocating mechanism, pinion and rack.
As shown in figure 3, the power source of bracket translating device 7 is bracket translation motor 71.Bracket translation motor 71 passes through support
Frame translation motor installing plate 72 is fixed on pedestal 13, and bracket translation motor 71 drives bracket translation screw mandrel 73 to rotate, and bracket is put down
Silk shifting rod 73 drives bracket translation feed screw nut 74 horizontal direction movement.The one side and bracket for driving bent plate 75 translate feed screw nut
74 are fixedly connected, and the another side of bending is fixedly connected with the intermediate support 85 in bracket elevating mechanism with bolt.Bracket translation is slided
Block 76 is arranged on intermediate support 85, and bracket translating rails 77 are fixed on pedestal 13.Bracket translator slider 76 is translated with bracket
The horizontal direction of guide rail 77 is slidably connected, and bracket translates feed screw nut 74 by driving bent plate 75 to drive intermediate support 85 flat along bracket
Guide rail 77 is moved to move horizontally.Now, the bracket lifting device 8 where intermediate support 85 with bracket system 9 in the horizontal direction without phase
To movement, both are an entirety.So driving bent plate 75 has driven bracket system 9 to move horizontally, bracket system 9 is moved horizontally
During bar magnet 20 for 21 bottoms of insertion stirring set state, above bar magnet 20 and stirring set 21 it is fit move horizontally i.e. Fig. 3
Shown d actions.D acts the carrying work for coordinating with above-mentioned a and e actions and completing magnetic bead.Bracket system 9 move horizontally because
The setting of bracket translator slider 76 and bracket translating rails 77 and it is more smooth.It is fixed on the bending thin slice 2 78 on driving bent plate 75
Coordinate with photoelectrical position sensor 57, realize the position signalling transmission that bracket system 9 is moved horizontally.
Bracket translates screw mandrel 73 and translates the leading screw and nut mechanism that feed screw nut 74 is constituted with bracket, can be lead screw
Other transmission mechanisms such as nut body, ball-screw nut mechanism, timing belt reciprocating mechanism, pinion and rack.
Further illustrate, in the state of bar magnet 20 is not inserted into stirring set 21, bracket lifting device 8 and bracket translating device
7 equally can drive bracket system 9 to be lifted and translated, and complete some auxiliary sexual acts of nucleic acid purification process.
Sliding block in stirring set drive mechanism 5, bar magnet elevating mechanism 6, bracket translating device 7, bracket lifting device 8 is with leading
Rail mechanism is rolling linear guide mechanism, and the frictional force between guide rail and sliding block is force of rolling friction, and resistance is smaller, movement parts
Motion it is more smooth, steady.
In summary, the bar magnet elevating mechanism 6 in bracket system 9 realizes the action that bar magnet 20 is individually lifted（A is acted）, will
Bar magnet 20 inserts or taken out stirring set 21.Bracket lifting device 8 and bracket translating device 7 realize bar magnet 20 and stirring set 21 respectively
Moving up and down after zoarium and translation motion（D is acted and e actions）, the carrying work of the above a, d, e action combination completion magnetic bead.
Stirring set drive mechanism 5 can drive stirring set 21 individually to move up and down and be stirred（B is acted and c actions）, complete stirring set
21 pass in and out deep holes and to the stirring work of magnetic bead in deep hole and liquid.Control system 2 according to the signal of photoelectrical position sensor 57,
Each motor action is controlled in an orderly manner, completes nucleic acid purification process.
Stirring set drive mechanism 5, bar magnet elevating mechanism 6, bracket translating device 7, bracket lifting device 8 can be independent for four
Control and the part of action, can complete various actions and the combination of sequence of movement, realize by changing the program of control system
Nucleic acid purification process, to meet the individual demand of user.
Although multiple embodiments of the present invention have been shown and described above, for one of ordinary skill in the art
For, it is possible to understand that without departing from the principles and spirit of the present invention these embodiments are carried out with a variety of changes, is repaiied
Change, replace and modification, protection scope of the present invention is limited by every claim and its equivalency range.
Claims (10)
1. a kind of nucleic acid purifier, including mechanical part（1）, control system（2）, operating unit（3）, shell（4）, the machinery
Part（1）Positioned at nucleic acid purifier bottom, the control system（2）With the operating unit（3）It is described outer positioned at instrument top
Shell（4）It is wrapped in instrument outermost, the mechanical part（1）Including stirring set drive mechanism（5）, stirring stock（10）, bar magnet
Elevating mechanism（6）, bar magnet frame（11）, support or supporting plate（12）, pedestal（13）, workbench（14）, translation device for operation table or
Support translating device, the stirring stock（10）It is installed on the stirring set drive mechanism（5）On, the bar magnet frame（11）Install
In the bar magnet elevating mechanism（6）On, the stirring set drive mechanism（5）With the bar magnet elevating mechanism（6）It is installed on support
Or supporting plate（12）On, support or supporting plate（12）With the workbench（14）It is installed on the pedestal（13）On, the work
Platform（14）It is arranged at the bar magnet frame（11）With the stirring stock（10）Lower section；
Characterized in that,
The stirring set drive mechanism（5）Including stirring motor（51）, crank（52）, connecting rod（53）, stirring set sliding block（54）, stir
Mix set guide rail（55）, the stirring motor（51）Axle and the crank（52）It is fixedly connected, the crank（52）With the connecting rod
（53）One end is rotatably connected, the connecting rod（53）The other end and the stirring set sliding block（54）It is rotatably connected, the stirring
Stock（10）It is fixed on the stirring set sliding block（54）On, the stirring set sliding block（54）With the stirring set guide rail（55）Slide
Connection.
2. nucleic acid purifier according to claim 1, it is characterised in that also including bracket lifting device（8）, the bracket
Lowering or hoisting gear（8）Including bracket lifting motor（81）, intermediate support（85）, bracket elevating screw（83）, bracket elevating screw spiral shell
It is female（84）, bracket lifting slider（87）, bracket riser guide（88）, the bracket lifting motor（81）It is installed in the middle of described and props up
Frame（85）On, the bracket elevating screw（83）With the bracket lifting motor（81）Axle is connected, the bracket elevating screw spiral shell
It is female（84）It is set in the bracket elevating screw（83）On, the bracket elevating screw nut（84）With the supporting plate（12）Even
Connect, the bracket lifting slider（87）Installed in the supporting plate（12）On, the bracket riser guide（88）It is fixed on described
Intermediate support（85）On, the bracket lifting slider（87）With the bracket riser guide（88）It is slidably connected.
3. nucleic acid purifier according to claim 2, it is characterised in that the support translating device is bracket translating device
（7）, the bracket translating device（7）Including bracket translation motor（71）, bracket translation screw mandrel（73）, bracket translation feed screw nut
（74）, bracket translator slider（76）, bracket translating rails（77）, the bracket translation motor（71）It is installed on the pedestal（13）
On, the bracket translates screw mandrel（73）With the bracket translation motor（71）Axle is connected, and the bracket translates feed screw nut（74）
It is set in the bracket translation screw mandrel（73）On, the bracket translates feed screw nut（74）With the bracket lifting device（8）Even
Connect, the bracket translator slider（76）Installed in the bracket lifting device（8）On, the bracket translating rails（77）It is arranged on
The pedestal（13）On, the bracket translator slider（76）With the bracket translating rails（77）It is slidably connected.
4. nucleic acid purifier according to claim 1, it is characterised in that the bar magnet elevating mechanism（6）Including bar magnet liter
Motor drops（61）, bar magnet elevating screw（62）, bar magnet elevating screw nut（63）, bar magnet lifting slider（64）, bar magnet lifting lead
Rail, the bar magnet lifting motor（61）Axle and the bar magnet elevating screw（62）Connection, the bar magnet elevating screw nut（63）
It is set in the bar magnet elevating screw（62）On, the bar magnet elevating screw nut（63）With the bar magnet frame（11）Connection, institute
State bar magnet lifting slider（64）It is fixed on the bar magnet frame（11）On, the bar magnet lifting slider（64）Led with bar magnet lifting
Rail is slidably connected.
5. nucleic acid purifier according to claim 1, it is characterised in that the stirring covers drive mechanism（5）Also include gear
Piece（56）, position sensor（57）, bearing（58）, the catch（56）It is fixed on crank（52）On, the position sensor
（57）It is fixed on the crank（52）Side, the catch（56）For thin plate, its thickness direction passes through the position sensor
（57）On groove, the bearing（58）It is installed on the connecting rod（53）Hole in.
6. nucleic acid purifier according to claim 5, it is characterised in that the catch（56）It is fan-shaped for right angle.
7. the nucleic acid purifier according to any claim in claim 1-4, it is characterised in that sliding block and guide rail mechanism
It is ball guiding rail mechanism.
8. nucleic acid purifier according to claim 1, it is characterised in that the stirring covers drive mechanism（5）The song
Handle（52）, the connecting rod（53）For aluminum alloy material.
9. nucleic acid purifier according to claim 1, it is characterised in that the crank（52）With the connecting rod（53）Length
Ratio is less than 1：1, the stirring motor（51）Frequency is 0 ~ 20Hz.
10. nucleic acid purifier according to claim 1, it is characterised in that the crank（52）With the connecting rod（53）It is long
It is 1 to spend ratio:4~1:6, the stirring motor（51）Frequency is 2 ~ 8Hz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710569179.6A CN107118958A (en) | 2017-07-13 | 2017-07-13 | Nucleic acid purifier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710569179.6A CN107118958A (en) | 2017-07-13 | 2017-07-13 | Nucleic acid purifier |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107118958A true CN107118958A (en) | 2017-09-01 |
Family
ID=59731177
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710569179.6A Pending CN107118958A (en) | 2017-07-13 | 2017-07-13 | Nucleic acid purifier |
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| Country | Link |
|---|---|
| CN (1) | CN107118958A (en) |
Cited By (8)
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| CN107674822A (en) * | 2017-11-17 | 2018-02-09 | 广州瑞能医学科技有限公司 | A kind of instrument for extracting nucleic acid |
| CN108982195A (en) * | 2018-08-31 | 2018-12-11 | 沭阳康源泰博生物科技有限公司 | A kind of full-automatic immunomagnetic beads method purifying instrument |
| WO2019011088A1 (en) * | 2017-07-13 | 2019-01-17 | 上海奇法生物仪器有限公司 | Nucleic acid purifier |
| CN110340506A (en) * | 2019-07-04 | 2019-10-18 | 佛山市昱纶机械有限公司 | A kind of bagged-spring welder |
| WO2020186601A1 (en) * | 2019-03-15 | 2020-09-24 | 深圳市易瑞生物技术股份有限公司 | Detection pretreatment device |
| CN112048429A (en) * | 2020-09-25 | 2020-12-08 | 杭州迪安生物技术有限公司 | High-efficient of nucleic acid extraction appearance is drawed and is shaken module and nucleic acid extraction appearance |
| CN113198206A (en) * | 2020-02-03 | 2021-08-03 | 瑞基海洋生物科技股份有限公司 | Biochemical reactor, stirring device thereof and extraction method |
| CN113943631A (en) * | 2020-07-15 | 2022-01-18 | 致茂电子(苏州)有限公司 | Method and equipment for mounting and detaching magnetic rod sleeve for nucleic acid extraction |
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| CN112048429A (en) * | 2020-09-25 | 2020-12-08 | 杭州迪安生物技术有限公司 | High-efficient of nucleic acid extraction appearance is drawed and is shaken module and nucleic acid extraction appearance |
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