CN107088235B

CN107088235B - Application of soapberry and extract thereof in preparation of hygienic products

Info

Publication number: CN107088235B
Application number: CN201610089822.0A
Authority: CN
Inventors: 王兵; 高鑫磊; 刘欣怡; 郑玉静; 陈尚义; 吕伟青
Original assignee: Beijing Taikemei Hi Tech Co ltd
Current assignee: Beijing Taikemei Hi Tech Co ltd
Priority date: 2016-02-17
Filing date: 2016-02-17
Publication date: 2020-11-03
Anticipated expiration: 2036-02-17
Also published as: CN107088235A

Abstract

The invention relates to the field of sanitary products, in particular to application of soapberry and an extract thereof in preparing sanitary products. The composition provided by the invention comprises a soapberry component, a compound component and an auxiliary material. The composition has good antibacterial and bacteriostatic effects, and has wide antibacterial spectrum and no damage to skin. The preparation method is simple and the cost is low. Experiments show that the composition has good inhibition effect on common bacteria or fungi in life, and the toxicity of the composition provided by the invention on epidermal cells is low.

Description

Application of soapberry and extract thereof in preparation of hygienic products

Technical Field

The invention relates to the field of sanitary products, in particular to application of soapberry and an extract thereof in preparing sanitary products.

Background

The sanitary articles refer to various daily necessities which are discarded after being used once, are in direct or indirect contact with a human body and are used for achieving the purposes of physiological sanitation or health care (antibiosis or bacteriostasis) of the human body, and the product characters can be solid or liquid. For example, sanitary products for excretions such as paper towels, wet wipes, feminine hygiene products (including pads), diapers, and the like (excluding toilet paper such as creped toilet paper).

The sanitary articles with the bacteriostatic or antibacterial function are mostly added with certain antibacterial components. At present, the antibacterial component added in the commercially available sanitary products is mainly chemical substances with antibacterial function, antibiotics or enzymes with antibacterial function. The long-term use of chemical substances or antibiotics as bacteriostatic components can reduce the immunity of human bodies and stimulate the skin to generate adverse reactions. The enzyme with the antibacterial effect does not influence the immunity of a human body, has a good antibacterial effect, but has unstable property, is easy to lose efficacy, has poor broad spectrum and only can inhibit certain specific microorganisms.

Therefore, it is necessary to develop an antibacterial agent suitable for sanitary goods.

Disclosure of Invention

In view of the above, the technical problem to be solved by the present invention is to provide an application of sapindus mukorossi and its extract in the preparation of hygienic products. The soapberry extract has the functions of sterilizing and inhibiting bacteria, relieving itching and diminishing inflammation; it can be used in sanitary products for antibacterial and anti-inflammatory effects.

The invention provides a sanitary product comprising a soapberry component, wherein the sanitary product is a sanitary towel, a protection pad, a diaper or a wet tissue; the fructus Sapindi Mukouossi component is fructus Sapindi Mukouossi or fructus Sapindi Mukouossi extract.

Sapindus mukorossi Gaertn (S. abrup-tus Lour.) A compendium of materia Medica is called Sapindus mukorossi. The peel of fructus Sapindi Mukouossi contains triterpenoid saponin such as sapindoside, has antibacterial, antiinflammatory and antioxidant effects, and can be used for cleaning tableware, caring skin, washing hair or caring skin.

The soapberry in the soapberry component refers to each part of a soapberry plant. Preferably, the fruit peel of Sapindus mukorossi. The composition is prepared by compounding the soapberry and other components, and has the functions of sterilization, bacteriostasis, itching relieving and inflammation diminishing. It has good bactericidal effect and broad bactericidal spectrum.

The sapindus mukorossi extract of the present invention refers to a substance extracted or processed from sapindus mukorossi (all or a part of the plant) by using a suitable solvent or method. Preferably, the sapindus mukorossi pericarp is extracted or processed. In the present invention, the sapindus mukorossi extract can be made by self or be purchased commercially, and the present invention is not limited thereto, but the implementation thereof is within the scope of the present invention.

In some embodiments, the solvent for extracting soapberry is water, aqueous ethanol solution or ethanol.

In some embodiments, the method of extraction is maceration extraction, percolation extraction, decoction extraction, soxhlet extraction, reflux extraction, microwave-assisted extraction, supercritical fluid extraction, or steam distillation extraction.

In the present invention, the soapberry extract includes a soapberry aqueous extract or a soapberry volatile oil.

Preferably, the preparation method of the sapindus mukorossi aqueous extract comprises the following steps: pulverizing peel of Sapindus mukurossi Mukurossi, extracting with water solution, standing the water extractive solution, and removing precipitate to obtain water extract of Sapindus mukurossi Mukurossi. Preferably, the extraction conditions are: extracting at 55 deg.C for 3 times (3 h, 2h, respectively) at a solid-liquid mass ratio of 1: 5.

Preferably, the preparation method of the sapindus mukorossi essential oil comprises the following steps: crushing soapberry peel, performing reflux extraction with ethanol, adjusting the pH value of an extracting solution to 9-10, and recovering ethanol to obtain a concentrated solution; extracting the concentrated solution with equal volume of diethyl ether for 3 times, and collecting water layer; adjusting pH of water layer to 5, extracting with equal volume of diethyl ether for 3 times, mixing extractive solutions, drying with sodium sulfate, and recovering diethyl ether in water bath. Preferably, the ethanol reflux extraction is carried out for 2 times, the first 5 times of ethanol extraction is carried out for 1.5 hours, and the second 3 times of ethanol extraction is carried out for 1 hour.

The preparation of the wet tissue by the soapberry extract comprises the following specific steps: mixing the Sapindus mukurossi extract with water to obtain soaking solution. And soaking the medical non-woven fabric in the soaking treatment solution, and draining to obtain the wet tissue. Preferably, the volume ratio of the soapberry extract to the water is 1: 9. Preferably, the nonwoven fabric is soaked in 200mL to 250mL of the soaking treatment solution per square meter of the nonwoven fabric.

The preparation of the sanitary towel, the panty liner or the diaper by using the extract comprises the following specific steps: mixing the Sapindus mukurossi extract with water to obtain soaking solution. Soaking medical non-woven fabric in the soaking solution, drying to obtain interlayer, and making into sanitary pad, sanitary towel or diaper. Preferably, the volume ratio of the soapberry extract to the water is 1: 9. Preferably, the nonwoven fabric is soaked in 200mL to 250mL of the soaking treatment solution per square meter of the nonwoven fabric.

In some embodiments, the compositions provided herein comprise a soapberry extract and an adjuvant.

In this example, the mass ratio of the soapberry extract to the auxiliary materials was 1: (3-5).

Preferably, the mass ratio of the soapberry extract to the auxiliary materials is 1: 4.

in this embodiment, the composition further comprises an adjuvant, wherein the adjuvant is cyclodextrin; the fructus Sapindi Mukouossi extract is fructus Sapindi Mukouossi volatile oil.

Experiments show that the composition has good bacteriostatic and bactericidal effects and a wide bactericidal spectrum, and has a remarkable killing effect on common bacteria in life.

The preparation of the sanitary towel, the panty liner or the diaper by using the composition comprises the following specific steps: mixing the volatile oil with cyclodextrin to obtain inclusion, and grinding to obtain medicinal powder core. The medicinal powder core is placed in the center of the middle layer of sanitary napkin, panty liner or diaper. Preferably, the composition is added in an amount of 0.2g to 5g per portion of sanitary napkin, pad or diaper.

The invention also provides a composition, which comprises a soapberry component and a compound component;

the compound components are selected from sodium hyaluronate, wormwood, fructus psoraleae, honeysuckle and tea polyphenol.

The fructus Sapindi Mukouossi component is fructus Sapindi Mukouossi or fructus Sapindi Mukouossi extract.

The tea polyphenol is polyphenol substances from tea leaves, and can be prepared by self or purchased in the market. It can be added into composition, and can be extracted product, or tea leaf and other components.

Experiments show that the composition provided by the invention has good bactericidal and bacteriostatic effects and a broad bactericidal spectrum. The antibacterial skin-care product has the function of preventing bacterial infection, has no damage to skin substances and immunity, and has positive significance for improving the health level of women, old people and children.

The invention also provides application of the composition in preparing sanitary products.

The invention also provides a sanitary product, which comprises the composition provided by the invention.

In the present invention, the sanitary article is a sanitary napkin, a panty liner, a diaper or a wet wipe.

In some embodiments, the compositions provided herein comprise soapberry extract and sodium hyaluronate.

In this embodiment, the mass ratio of the soapberry extract to the sodium hyaluronate is 20 (0.5-1.5).

Preferably, the mass ratio of the soapberry extract to the sodium hyaluronate is 20: 1.

In this embodiment, the soapberry extract is an aqueous soapberry extract; the composition also comprises auxiliary materials, wherein the auxiliary materials comprise a preservative and essence.

The preservative is selected from benzoic acid, sodium benzoate, sorbic acid, potassium sorbate, calcium propionate. The mass fraction of the preservative is 0.02%.

The essence is selected from jasmine essence, osmanthus essence, rose essence, mint essence, lavender essence, vanilla essence, gardenia essence, magnolia alba essence, strawberry essence, apple essence, lemon essence, peach essence, chocolate essence, cologne essence, mint essence, milk essence, coffee essence, sandalwood essence, camphorwood essence, pine essence, eucalyptus essence or cedar essence. The mass fraction of the essence is 0.02%.

Experiments show that the composition has good bacteriostatic and bactericidal effects, wide bactericidal spectrum and obvious killing and removing effects on common bacteria in life. In addition, the composition has certain effect of protecting skin, and does not damage skin cells. Indicating that the composition can be used in hygiene products.

The preparation of the wet tissue by using the composition comprises the following specific steps: mixing fructus Sapindi Mukouossi extract, sodium hyaluronate and water, adding essence and antiseptic, and making into soaking solution. And soaking the medical non-woven fabric in the soaking treatment solution, and draining to obtain the wet tissue. Preferably, the volume ratio of the soapberry extract to the water is 1: 9. Preferably, the nonwoven fabric is soaked in 200mL to 250mL of the soaking treatment solution per square meter of the nonwoven fabric.

The preparation of the sanitary towel, the panty liner or the diaper by using the composition comprises the following specific steps: mixing fructus Sapindi Mukouossi extract, sodium hyaluronate and water to obtain soaking solution. Soaking medical non-woven fabric in the soaking solution, drying to obtain interlayer, and making into sanitary pad, sanitary towel or diaper. Preferably, the volume ratio of the soapberry extract to the water is 1: 9. Preferably, the nonwoven fabric is soaked in 200mL to 250mL of the soaking treatment solution per square meter of the nonwoven fabric.

In some embodiments, the compositions provided herein comprise soapberry, wormwood, honeysuckle, tea polyphenols.

In this embodiment, the mass ratio of Sapindus mukurossi, mugwort, honeysuckle flower and psoralea fruit is (12-8): 1-3): 1-5.

Preferably, the mass ratio of the soapberry, the wormwood, the honeysuckle and the fructus psoraleae is 10:2:2: 4.

Experiments show that the composition has good bacteriostatic and bactericidal effects and a wide bactericidal spectrum, and has a remarkable killing effect on common bacteria in life. Indicating that the composition can be used in hygiene products.

The preparation of the sanitary wet tissue by using the composition comprises the following specific steps: mixing fructus Sapindi Mukouossi pericarp, folium Artemisiae Argyi, flos Lonicerae, and fructus Psoraleae with water, and leaching to obtain soaking solution. And soaking the medical non-woven fabric in the soaking treatment solution, and draining to obtain the wet tissue. Preferably, the leaching temperature is 20-50 ℃, and the leaching time is 5-8 h. Preferably, the mass-volume ratio of the sum of the masses of the soapberry peel, the folium artemisiae argyi, the honeysuckle and the fructus psoraleae to water is (12-13): 9. Preferably, the nonwoven fabric is soaked in 200mL to 250mL of the soaking treatment solution per square meter of the nonwoven fabric.

The preparation of the sanitary towel, the panty liner or the diaper by using the composition comprises the following specific steps: mixing fructus Sapindi Mukouossi pericarp, folium Artemisiae Argyi, flos Lonicerae, and fructus Psoraleae with water, and leaching to obtain soaking solution. Soaking medical non-woven fabric in the soaking solution, drying to obtain interlayer, and making into sanitary pad, sanitary towel or diaper. The leaching temperature is 2-40 ℃, and the leaching time is 5-8 h. Preferably, the volume ratio of the soapberry extract to the water is 1: 9. Preferably, the nonwoven fabric is soaked in 200mL to 250mL of the soaking treatment solution per square meter of the nonwoven fabric.

In some embodiments, the compositions provided herein comprise soapberry extract and tea polyphenols.

In this example, the mass ratio of the soapberry extract to the tea polyphenol was 1: (0.2 to 1.5).

Preferably, the mass ratio of the soapberry extract to the tea polyphenol is 1: 1.

In this example, the mass ratio of the soapberry extract, the tea polyphenol and the cyclodextrin was 1:1: 4.

Experiments show that the composition has good bacteriostatic and bactericidal effects and a wide bactericidal spectrum, and has a remarkable killing effect on common bacteria in life. In addition, the composition can relieve discomfort caused by long-term use of sanitary articles. Indicating that the composition can be used in hygiene products.

The preparation of the sanitary towel, the panty liner or the diaper by using the composition comprises the following specific steps: mixing the fructus Sapindi Mukouossi volatile oil and cyclodextrin to obtain inclusion, mixing the inclusion with tea polyphenols, and grinding to obtain powder core. The medicinal powder core is placed in the center of the middle layer of sanitary napkin, panty liner or diaper. Preferably, the composition is added in an amount of 0.2g to 5g per portion of sanitary napkin, pad or diaper.

The sanitary product provided by the invention comprises the soapberry extract, so that the sanitary product has good bactericidal property. The composition provided by the invention comprises a soapberry component, a compound component and an auxiliary material. The composition has good antibacterial and bacteriostatic effects, and has wide antibacterial spectrum and no damage to skin. The preparation method is simple and the cost is low. Experiments show that the composition has good inhibition effect on common bacteria or fungi in life, and the toxicity of the composition provided by the invention on epidermal cells is low.

Detailed Description

The invention provides the application of the soapberry and the extract thereof in the preparation of hygienic products, and the technical personnel can appropriately improve the process parameters by referring to the content. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.

The materials, reagents or instruments adopted by the invention are all common products sold on the market and can be purchased on the market.

The invention is further illustrated by the following examples:

example 1

Pulverizing peel of Sapindus mukurossi Mukurossi, extracting with water solution, standing the water extractive solution, and removing precipitate to obtain water extract of Sapindus mukurossi Mukurossi. Preferably, the extraction conditions are: extracting at 55 deg.C for 3 times (3 hr, 2 hr) at solid-liquid mass ratio of 1:5,

mixing 1L fructus Sapindi Mukouossi extract with 9L water to obtain soaking solution.

And soaking the medical non-woven fabric in the soaking treatment solution, and draining to obtain the wet tissue. Each square meter of the non-woven fabric is soaked in 200-250 mL of soaking treatment solution.

Example 2

mixing 1L of Sapindus mukurossi extract with 9L of water. Soaking medical non-woven fabric in the soaking solution, drying to obtain interlayer, and making into sanitary pad, sanitary towel or diaper. Each square meter of the non-woven fabric is soaked in 200-250 mL of soaking treatment solution.

Example 3

mixing 1L of fructus Sapindi Mukouossi extract, 50g of sodium hyaluronate and 9L of water, and adding 0.2g of rose essence and 0.2g of benzoic acid antiseptic to obtain soaking solution.

Example 4

1L of soapberry extract, 50g of sodium hyaluronate and 9L of water were mixed. Soaking medical non-woven fabric in the soaking solution, drying to obtain interlayer, and making into sanitary pad, sanitary towel or diaper. Each square meter of the non-woven fabric is soaked in 200-250 mL of soaking treatment solution.

Example 5

Mixing 10kg of soapberry peel, 2kg of folium artemisiae argyi, 2kg of fructus psoraleae, 1kg of tea and 1kg of honeysuckle with 9L of water, and leaching at 20 ℃ for 8h to prepare a soaking treatment solution. And soaking the medical non-woven fabric in the soaking treatment solution, and draining to obtain the wet tissue. Each square meter of the non-woven fabric is soaked in 200-250 mL of soaking treatment solution.

Example 6

Mixing 10kg of soapberry peel, 2kg of folium artemisiae argyi, 2kg of fructus psoraleae, 1kg of honeysuckle, 50g of tea polyphenol and 1kg of honeysuckle with 9L of water, and leaching at 50 ℃ for 5h to prepare a soaking treatment solution. Soaking medical non-woven fabric in the soaking solution, drying to obtain interlayer, and making into sanitary pad, sanitary towel or diaper. Each square meter of the non-woven fabric is soaked in 200-250 mL of soaking treatment solution.

Example 7

Crushing soapberry peel, performing reflux extraction with ethanol, adjusting the pH value of an extracting solution to 9-10, and recovering ethanol to obtain a concentrated solution; extracting the concentrated solution with equal volume of diethyl ether for 3 times, and collecting water layer; adjusting pH of water layer to 5, extracting with equal volume of diethyl ether for 3 times, mixing extractive solutions, drying with sodium sulfate, and recovering diethyl ether in water bath. Preferably, the ethanol reflux extraction is carried out for 2 times, the first 5 times of ethanol extraction is carried out for 1.5 hours, and the second 3 times of ethanol extraction is carried out for 1 hour.

Adding 4kg of cyclodextrin into 1L of fructus Sapindi Mukouossi volatile oil, and stirring to obtain inclusion of fructus Sapindi Mukouossi volatile oil. Is used as a medicinal powder core and is arranged in the center of middle layers of sanitary napkins, panty liners and diapers.

Example 8

Adding 4kg of cyclodextrin into 1L of fructus Sapindi Mukouossi volatile oil, and stirring to obtain inclusion of fructus Sapindi Mukouossi volatile oil. Adding 1kg tea polyphenols into the inclusion, and mixing. Is used as a medicinal powder core and is arranged in the center of middle layers of sanitary napkins, panty liners and diapers.

Example 9

The antibacterial performance of the composition provided by the invention is detected by a bacteriostatic circle method, and the experimental strains are staphylococcus aureus, bacillus subtilis, bacillus cereus, escherichia coli, proteus vulgaris, bacillus megaterium, candida albicans, aspergillus flavus, aspergillus niger, trichoderma koningii, penicillium and trichoderma viride.

Melting solid culture medium suitable for growth and development of test bacteria, cooling to about 45-50 ℃, mixing prepared strain suspension 10mL with the culture medium, adding the culture medium with bacteria on the solidified solid culture medium, and coating 5 culture dishes with each strain solution. Example 1, example 3, example 5; the powder cores obtained in examples 2, 4, 6, and 7 to 8 were formed into a cylinder having a diameter of 0.5mm, and a blank control cylinder was provided. These 9 small cylinders were placed on a medium at appropriate intervals and cultured at an appropriate temperature.

And (3) under an appropriate condition, culturing for 2-3 days, taking out, measuring the diameter of the inhibition zone by using a cross method, and evaluating the bactericidal activity according to the existence and the size of the inhibition zone. If the diameter of the inhibition zone is elliptic, the difference of the length is more than one unit, and the inhibition zone is preferably left out. Each experimental strain was replicated 3 times. The results are shown in Table 1:

TABLE 1 average diameter of zone of inhibition (mm)

	Staphylococcus aureus	Escherichia coli	Candida albicans	Aspergillus flavus	Aspergillus niger	Penicillium mould
							Blank control	6	6	6	6	6	6
Example 1	11	12	16	14	15	13
							Example 2	13	13	16	15	16	15
Example 3	15	15	17	17	18	17
							Example 4	16	15	18	16	18	18
Example 5	21	21	27	25	30	26
							Example 6	23	21	28	26	32	25
Example 7	17	17	19	20	22	19
							Example 8	19	18	21	21	23	21

The results show that the substances prepared in the examples can form a zone of inhibition, the diameter of which is significantly larger than that of the blank control, and the substances have good antibacterial effect. The most effective of these is example 6.

Example 10

Culturing human skin fibroblast in DMEM culture solution containing 10% fetal calf serum, collecting cells in logarithmic growth phase, and culturing at 4 × 10³The density of each well, inoculating in a 96-well plate, culturing until the wall is attached, removing the culture solution, randomly dividing into 9 groups,

DMEM medium was added to each group. One group was blank control, and the other 8 groups were experimental groups. The substances provided by the embodiment of the invention are added into each experimental group. The addition amount is as follows: 5mL of the immersion treatment solution prepared in examples 1 to 6 was added to 100mL of the culture solution; 5g of the powder cores prepared in examples 7 to 8 were added to 100mL of the culture medium.

Each group is provided with 10 compound holes which are placed in 5 percent CO₂Culturing in 37 deg.C saturated humidity incubator, and measuring OD after 72 hr₄₅₀Values to compare proliferation of the cells of each group. The results are shown in Table 2:

TABLE 2 OD of each group₄₅₀Value of

	Blank control	Example 1	Example 2	Example 3	Example 4	Example 5	Example 6	Example 7	Example 8
										OD₄₅₀	0.570	0.565	0.566	0.567	0.568	0.566	0.565	0.567	0.564

The results show that the proliferation of the cells in each of examples 1-6 is similar to that of the blank control, and there is no significant difference, which indicates that the composition provided by the present invention does not affect the growth of the cells.

Example 11

100 cases of female patients are treated together, and the age is 18-39 years. Clinically, the vagina, the pelvic inflammation, the endometritis, the cervicitis, the pruritus and the leucorrhea abnormality are seen, and the diagnosis is confirmed by removing the blockage of the oviduct and other organic diseases of the pelvic cavity.

Patients were randomly divided into 5 groups, and sanitary napkins or pads prepared in examples 2, 4, 6-8 were used during the period of the example vacation, respectively, with an experimental period of 6 months.

The curative effect standard is as follows:

the effect is shown: the symptoms such as clinical inflammation, pruritus, abnormal leucorrhea and the like are obviously improved;

the method has the following advantages: the symptoms of clinical inflammation, pruritus, abnormal leucorrhea and the like are reduced;

and (4) invalidation: the symptoms such as clinical inflammation, pruritus and abnormal leucorrhea are not improved at all.

The treatment effect showed that after 6 months the statistical results are shown in table 3:

TABLE 3 therapeutic Effect

	Show effect	Is effective	Invalidation
				Example 4	35	57	8
Example 6	49	48	3
				Example 7	44	51	5
Example 8	46	50	4
				Example 2	32	46	22

Therefore, the composition provided by the invention has the effects of killing fungi and diminishing inflammation.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that it is obvious to those skilled in the art that various modifications and improvements can be made without departing from the principle of the present invention, and these modifications and improvements should also be considered as the protection scope of the present invention.

Claims

1. A composition, characterized in that it is prepared by a process comprising:

mixing 10kg of soapberry peel, 2kg of folium Artemisiae Argyi, 2kg of fructus Psoraleae, 1kg of flos Lonicerae and 50g of tea polyphenols with 9L of water, and leaching at 50 deg.C for 5 h.

2. Use of a composition according to claim 1 for the preparation of a sanitary article.

3. A sanitary article comprising the composition of claim 1, wherein the sanitary article is a sanitary napkin, a panty liner, a diaper or a wet wipe.