CN107080770A - Prevent and treat the compound Chinese medicine and its preparation and application of fish doctylogyrosis - Google Patents
Prevent and treat the compound Chinese medicine and its preparation and application of fish doctylogyrosis Download PDFInfo
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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- A61K36/88—Liliopsida (monocotyledons)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
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- A61K36/9068—Zingiber, e.g. garden ginger
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Abstract
The invention discloses a kind of compound Chinese medicine for preventing and treating fish doctylogyrosis, main raw material includes：CORTEX AILANTHI, play both sides of the street and pumpkin seeds, the CORTEX AILANTHI can effectively kill Dactylogyrus and worm's ovum in the early stage of preventing and treating of fish doctylogyrosis；Described play both sides of the street in prevent and treat early stage and the later stage of fish doctylogyrosis has more significant killing effect to Dactylogyrus and worm's ovum；The pumpkin seeds have effects that the desinsection that detoxifies, and mainly act on the preventing and treating later stage of fish doctylogyrosis, Dactylogyrus and worm's ovum to kill a small amount of remnants；By CORTEX AILANTHI, the compound Chinese medicine played both sides of the street with pumpkin seeds compatibility, the preventing and treating that can take into account fish doctylogyrosis is whole；The compound Chinese medicine of preventing and treating fish doctylogyrosis of the present invention, it not only can effectively prevent and treat fish doctylogyrosis, improve breeding survival rate, solve the problem of Dactylogyrus easily produces resistance for simple medicine, harmful microbe metabolism growth in breeding water body can also be suppressed to a certain extent, so as to improve the quality of breeding water body.
Description
Technical field
The present invention relates to freshwater fish culturing control and prevention of disease field, it is more particularly related to which a kind of preventing and treating fish refer to
The compound Chinese medicine of ring parasitosis.
Background technology
Doctylogyrosis is one of disease of harm most serious in fish culture, by exciting Infective after Dactylogyrus is parasitic
The clinical symptoms for the fish " gill rot " that bacterium and fungi are caused cause cultured fishes mortality.In view of Dactylogyrus is easily produced
Drug resistance, causes conventional national standard medicine worse and worse, to cause the medicine for preventing and treating fish doctylogyrosis indiscriminate Dactylogyrus prevention effect
With phenomenon generally, it is to cause one of main inducing of cultivation water environment destruction and fish quality, therefore, initiative is new to be prevented
Controlling the safely, effectively medicine of fish doctylogyrosis turns into the task of top priority.Chinese herbal medicine is because it is to animal safety, Small side effects and to ring
The characteristics of border is gentle, the focus as Development of New Drugs.And in face of resistance problem of the Dactylogyrus for simple medicine, drug combination tool
There are greater advantages.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantage that at least will be described later.
It is a still further object of the present invention to provide a kind of compound Chinese medicine for preventing and treating fish doctylogyrosis, not only can effectively it prevent
Control fish doctylogyrosis, improve breeding survival rate, solve the problem of Dactylogyrus easily produces resistance for simple medicine, can also be
Suppress harmful microbe metabolism growth in breeding water body to a certain extent, so as to improve the quality of breeding water body.
Answering for fish doctylogyrosis is prevented and treated there is provided a kind of according to object of the present invention and further advantage in order to realize
Chinese medicine is closed, mainly includes the raw material of following parts by weight：
10-30 parts of CORTEX AILANTHI, play both sides of the street 10-30 parts and 10-70 parts of pumpkin seeds.
Preferably, the compound Chinese medicine of described preventing and treating fish doctylogyrosis, includes the auxiliary material of following parts by weight：Garlic
1-5 parts of powder, 1-3 parts of matrimony vine, 1-3 parts of the bark of eucommia, 1-7 parts of Verbena officinalis, 1-3 parts of radix scutellariae, 1-7 parts of rhizoma zingiberis, 1-3 parts of the tuber of stemona, kuh-seng 1-7
Part, 1-3 parts of granatum, 1-3 parts of Paris polyphylla and 1-5 parts of green bristlegrass.
Preferably, the compound Chinese medicine of described preventing and treating fish doctylogyrosis, includes the raw material of following parts by weight：CORTEX AILANTHI
20 parts, play both sides of the street 20 parts, 40 parts of pumpkin seeds, 3 parts of garlic powder, 2 parts of matrimony vine, 2 parts of the bark of eucommia, 4 parts of Verbena officinalis, 2 parts of radix scutellariae, rhizoma zingiberis 4
Part, 2 parts of the tuber of stemona, 4 parts of kuh-seng, 2 parts of granatum, 2 parts of Paris polyphylla and 3 parts of green bristlegrass.
Present invention also offers a kind of preparation method for the compound Chinese medicine for preventing and treating fish doctylogyrosis, comprise the following steps：
Step 1: by CORTEX AILANTHI, play both sides of the street, pumpkin seeds, garlic powder, matrimony vine, the bark of eucommia, Verbena officinalis, radix scutellariae, rhizoma zingiberis, the tuber of stemona,
Kuh-seng, granatum, Paris polyphylla and green bristlegrass are crushed to more than 30 mesh standby respectively.
Step 2: the various raw materials that step one is crushed cross more than 30 mesh sieves respectively, well mixed that Solid powder agent is made is standby
With.
Step 3: the Solid powder agent is soluble in water, by gradient increased temperature progress water extraction, mistake in the range of 50-100 DEG C
Night simultaneously filters the dregs of a decoction, produces the extract solution of the compound Chinese medicine of the preventing and treating fish doctylogyrosis.
Preferably, it is described in the preparation method of the compound Chinese medicine of described preventing and treating fish doctylogyrosis, the step one
CORTEX AILANTHI, play both sides of the street, pumpkin seeds, garlic powder, matrimony vine, the bark of eucommia, Verbena officinalis, radix scutellariae, rhizoma zingiberis, the tuber of stemona, kuh-seng, granatum, Paris polyphylla
And fully dried before green bristlegrass crushing.
Preferably, it is described in the preparation method of the compound Chinese medicine of described preventing and treating fish doctylogyrosis, the step 2
The various raw materials crushed cross that 40 mesh sieves are well mixed to be made Solid powder agent respectively.
Preferably, it is described in the preparation method of the compound Chinese medicine of described preventing and treating fish doctylogyrosis, the step 3
Thermograde is：60 DEG C of constant temperature 2h, 70 DEG C of constant temperature 2h, 80 DEG C of constant temperature 2h, the time overnight is minimum 6h.
Present invention also offers a kind of application method for the compound Chinese medicine for preventing and treating fish doctylogyrosis, in described will being combined
Medicine is put into breeding water body and used, and injected volume is using the final concentration of 40-100mg/L of the raw material as standard.
The present invention at least includes following beneficial effect：
The present invention is using CORTEX AILANTHI, play both sides of the street the active component main as Dactylogyrus is killed with pumpkin seeds, the Chinese toon root
Skin can effectively kill Dactylogyrus and worm's ovum in the early stage of preventing and treating of fish doctylogyrosis, and its killing rate is more than 92%；The road road
Lead to has more significant killing effect in prevent and treat early stage and the later stage of fish doctylogyrosis to Dactylogyrus and worm's ovum；The pumpkin seeds
There is the desinsection that detoxifies, its killing rate is more than 92%, the preventing and treating later stage of fish doctylogyrosis is mainly acted on, to kill
A small amount of remaining Dactylogyrus and worm's ovum；By CORTEX AILANTHI, the compound Chinese medicine played both sides of the street with pumpkin seeds compatibility, fish can be taken into account
The preventing and treating whole process of class doctylogyrosis kills Dactylogyrus adult and worm's ovum, and its killing rate is up to 97%；The compound Chinese medicine not only may be used
Effectively to prevent and treat fish doctylogyrosis, breeding survival rate is improved, Dactylogyrus is solved and easily produces asking for resistance for simple medicine
Topic, can also suppress harmful microbe metabolism growth in breeding water body to a certain extent, so as to improve the quality of breeding water body.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Brief description of the drawings
Fig. 1 compound Chinese medicines described in claim 1 in the present invention were in the 3rd day killing effect statistical chart to Dactylogyrus；
Fig. 2 compound Chinese medicines described in claim 1 in the present invention were in the 8th day killing effect statistical chart to Dactylogyrus；
Fig. 3 compound Chinese medicines described in claim 2 in the present invention were in the 3rd day killing effect statistical chart to Dactylogyrus；
Fig. 4 compound Chinese medicines described in claim 2 in the present invention were in the 8th day killing effect statistical chart to Dactylogyrus.
Embodiment
The present invention is described in further detail below, to make those skilled in the art being capable of evidence with reference to specification word
To implement.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or many
The presence or addition of individual other elements or its combination.
The present invention provides a kind of compound Chinese medicine for preventing and treating fish doctylogyrosis, includes the raw material of following parts by weight：CORTEX AILANTHI
10-30 parts, play both sides of the street 10-30 parts and 10-70 parts of pumpkin seeds.
In such scheme, using CORTEX AILANTHI, play both sides of the street with pumpkin seeds as the main active component for killing Dactylogyrus,
As shown in Figures 1 and 2, the CORTEX AILANTHI can effectively kill Dactylogyrus and worm's ovum in the early stage of preventing and treating of fish doctylogyrosis, its
Insecticidal effect is more than 92%；It is described play both sides of the street fish doctylogyrosis prevent and treat early stage and later stage have to Dactylogyrus and worm's ovum compared with
Significant killing effect；The pumpkin seeds have effects that the desinsection that detoxifies, and its insecticidal effect is more than 92%, mainly acts on fish
The preventing and treating later stage of doctylogyrosis, Dactylogyrus and worm's ovum to kill a small amount of remnants；By CORTEX AILANTHI, play both sides of the street and pumpkin seeds compatibility
The compound Chinese medicine, the preventing and treating whole process that can take into account fish doctylogyrosis kills Dactylogyrus adult and worm's ovum, its killing rate
Reach as high as 97%；Fish doctylogyrosis not only can be effectively prevented and treated, breeding survival rate is improved, Dactylogyrus is solved for simple medicine
The problem of thing easily produces resistance, can also suppress harmful microbe metabolism growth in breeding water body to a certain extent, so that
Improve the quality of breeding water body.
In one preferred scheme, the described compound Chinese medicine for preventing and treating fish doctylogyrosis, in addition to following parts by weight are auxiliary
Material：1-5 parts of garlic powder, 1-3 parts of matrimony vine, 1-3 parts of the bark of eucommia, 1-7 parts of Verbena officinalis, 1-3 parts of radix scutellariae, 1-7 parts of rhizoma zingiberis, 1-3 parts of the tuber of stemona,
1-5 parts of 1-7 parts of kuh-seng, 1-3 parts of granatum, 1-3 parts of Paris polyphylla and green bristlegrass.
In such scheme, the garlic powder, the bark of eucommia and radix scutellariae compatibility have broad-spectrum antiseptic, antibacterial and bactericidal action, can
Suppress the growth of microorganism in breeding water body to a certain extent, be not susceptible to scabies secondary infection, effectively improve survival rate, horsewhip
Grass, rhizoma zingiberis, the tuber of stemona, kuh-seng and Paris polyphylla compatibility can improve disinsection efficiency, and the granatum and green bristlegrass compatibility can effectively treat fish
The gill rot that class triggers by infection Dactylogyrus, the matrimony vine can slow down the accumulating rate of organic matter in breeding water body, be effectively improved water
Matter, as shown in Figure 3 or Figure 4, the compound Chinese medicine added with auxiliary materials such as garlic powder, matrimony vine, the barks of eucommia have higher insecticidal effect.
In one preferred scheme, the described compound Chinese medicine for preventing and treating fish doctylogyrosis, wherein, including following parts by weight
Raw material：20 parts of CORTEX AILANTHI, play both sides of the street 20 parts, it is 40 parts of pumpkin seeds, 3 parts of garlic powder, 2 parts of matrimony vine, 2 parts of the bark of eucommia, 4 parts of Verbena officinalis, yellow
3 parts of 2 parts of a kind of reed mentioned in ancient books, 4 parts of rhizoma zingiberis, 2 parts of the tuber of stemona, 4 parts of kuh-seng, 2 parts of granatum, 2 parts of Paris polyphylla and green bristlegrass.
Present invention also offers a kind of preparation method for the compound Chinese medicine for preventing and treating fish doctylogyrosis, wherein, including it is following
Step：
Step 1: by CORTEX AILANTHI, play both sides of the street, pumpkin seeds, garlic powder, matrimony vine, the bark of eucommia, Verbena officinalis, radix scutellariae, rhizoma zingiberis, the tuber of stemona,
Kuh-seng, granatum, Paris polyphylla and green bristlegrass are crushed to more than 30 mesh standby respectively.
Step 2: the various raw materials that step one is crushed cross more than 30 mesh sieves respectively, well mixed that Solid powder agent is made is standby
With.
Step 3: the Solid powder agent is soluble in water, by gradient increased temperature progress water extraction, mistake in the range of 50-100 DEG C
Night simultaneously filters the dregs of a decoction, produces the extract solution of the compound Chinese medicine of the preventing and treating fish doctylogyrosis.
In one preferred scheme, the preparation method of the compound Chinese medicine of described preventing and treating fish doctylogyrosis, institute in step one
State CORTEX AILANTHI, play both sides of the street, pumpkin seeds, garlic powder, matrimony vine, the bark of eucommia, Verbena officinalis, radix scutellariae, rhizoma zingiberis, the tuber of stemona, kuh-seng, granatum, again
Building and green bristlegrass are fully dried before crushing.
In such scheme, dried medicinal material moisture is extremely low, it is easier to crush uniformly for little particle cross 30 mesh with
Upper sieve.
In one preferred scheme, the preparation method of the compound Chinese medicine of described preventing and treating fish doctylogyrosis, institute in step 2
State the various raw materials of crushing and cross that 40 mesh sieves are well mixed to be made Solid powder agent respectively.
In such scheme, the Solid powder agent is conveniently stored and used.
In one preferred scheme, the preparation method of the compound Chinese medicine of described preventing and treating fish doctylogyrosis, institute in step 3
Stating thermograde is：60 DEG C of constant temperature 2h, 70 DEG C of constant temperature 2h, 80 DEG C of constant temperature 2h, the time overnight is minimum 6h.
In such scheme, water extraction is carried out by design temperature gradient from low to high, various medicine groups can not destroyed
Effective ingredient is fully extracted on the premise of point.
Present invention also offers a kind of application method for the compound Chinese medicine for preventing and treating fish doctylogyrosis, in described will being combined
Medicine is put into breeding water body and used, and injected volume is using the final concentration of 40-100mg/L of the raw material as standard.
Embodiment 1
A kind of compound Chinese medicine for preventing and treating fish doctylogyrosis, mainly includes the raw material of following parts by weight：CORTEX AILANTHI 10, Lu Lu
Lead to 10 and pumpkin seeds 10.
Step 1: by CORTEX AILANTHI, play both sides of the street, that pumpkin seeds are crushed to 30 mesh respectively is standby.
Step 2: the various raw materials that step one is crushed cross 30 mesh sieves respectively, well mixed that Solid powder agent is made is standby.
Step 3: the Solid powder agent is soluble in water, by 50 DEG C of constant temperature 2h of thermograde, 60 DEG C of constant temperature 2h, 70 DEG C
Constant temperature 2h carries out water extraction, stays overnight and filters the dregs of a decoction, produces the extract solution of the compound Chinese medicine of the preventing and treating fish doctylogyrosis.
Embodiment 2
A kind of compound Chinese medicine for preventing and treating fish doctylogyrosis, mainly includes the raw material of following parts by weight：CORTEX AILANTHI 20, Lu Lu
Lead to 20 and pumpkin seeds 40.
Step 1: by CORTEX AILANTHI, play both sides of the street, that pumpkin seeds are crushed to 40 mesh respectively is standby.
Step 2: the various raw materials that step one is crushed cross 40 mesh sieves respectively, well mixed that Solid powder agent is made is standby.
Step 3: the Solid powder agent is soluble in water, by 60 DEG C of constant temperature 2h of thermograde, 70 DEG C of constant temperature 2h, 80 DEG C
Constant temperature 2h carries out water extraction, stays overnight and filters the dregs of a decoction, produces the extract solution of the compound Chinese medicine of the preventing and treating fish doctylogyrosis.
Embodiment 3
A kind of compound Chinese medicine for preventing and treating fish doctylogyrosis, mainly includes the raw material of following parts by weight：CORTEX AILANTHI 30, Lu Lu
Lead to 30 and pumpkin seeds 70.
Step 1: by CORTEX AILANTHI, play both sides of the street, that pumpkin seeds are crushed to 50 mesh respectively is standby.
Step 2: the various raw materials that step one is crushed cross 50 mesh sieves respectively, well mixed that Solid powder agent is made is standby.
Step 3: the Solid powder agent is soluble in water, by 70 DEG C of constant temperature 2h of thermograde, 80 DEG C of constant temperature 2h, 90 DEG C
Constant temperature 2h carries out water extraction, stays overnight and filters the dregs of a decoction, produces the extract solution of the compound Chinese medicine of the preventing and treating fish doctylogyrosis.
Embodiment 4
A kind of compound Chinese medicine for preventing and treating fish doctylogyrosis, mainly includes the raw material of following parts by weight：CORTEX AILANTHI 20, Lu Lu
Logical 20, pumpkin seeds 40, garlic powder 3, matrimony vine 1, the bark of eucommia 1, Verbena officinalis 4, radix scutellariae 1, rhizoma zingiberis 4, the tuber of stemona 1, kuh-seng 4, granatum 1, again
Building 1 and green bristlegrass 3.
Step 1: by CORTEX AILANTHI, play both sides of the street, pumpkin seeds, garlic powder, matrimony vine, the bark of eucommia, Verbena officinalis, radix scutellariae, rhizoma zingiberis, the tuber of stemona,
It is standby that kuh-seng, granatum, Paris polyphylla and green bristlegrass are crushed to 40 mesh respectively.
Step 2: the various raw materials that step one is crushed cross 40 mesh sieves respectively, well mixed that Solid powder agent is made is standby.
Step 3: the Solid powder agent is soluble in water, by 60 DEG C of constant temperature 2h of thermograde, 70 DEG C of constant temperature 2h, 80 DEG C
Constant temperature 2h carries out water extraction, stays overnight and filters the dregs of a decoction, produces the extract solution of the compound Chinese medicine of the preventing and treating fish doctylogyrosis.
Effectiveness of the invention is described further below by experiment.
Experiment one
In order to observe effect of the compound Chinese medicine single component to Dactylogyrus adult and worm's ovum, 2.5g Chinese toon roots are weighed respectively
Skin, play both sides of the street, pumpkin seeds Solid powder agent, be dissolved in respectively in 200mL water, by 60 DEG C of constant temperature 2h of thermograde, 70 DEG C of constant temperature 2h,
80 DEG C of constant temperature 2h stay overnight water extraction, remove and 50mL medicine stostes are concentrated into after filter residue.
In order to observe effect of the compound Chinese medicine composition to Dactylogyrus adult and worm's ovum, 0.625g Chinese toons are weighed respectively
Root skin, 0.625g play both sides of the street and 1.25g pumpkin seeds are crossed after 40 mesh sieves are mixed and obtain the Solid powder agent, by the solid powder
Last agent is dissolved in 200mL water, is stayed overnight water extraction by 60 DEG C of constant temperature 2h of thermograde, 70 DEG C of constant temperature 2h, 80 DEG C of constant temperature 2h, is removed filter residue
After be concentrated into 50mL compound Chinese medicine stostes.
Add in aquarium after 50L water, aeration 8h, every group takes 10 goldfish temporarily to support after 24h, be separately added into medicine stoste,
Compound Chinese medicine stoste (blank control group is addition 50mL water), the final concentration of 50mg/L of medicine, 25 DEG C of experimental temperature.Due to finger ring
The generation cycle of worm is 8 days or so, and experiment sample time is respectively the 3rd day and the 8th day.
The single component medicine of table 1 and combination drug act on Dactylogyrus quantity after the 3rd day and the 8th day
By above-mentioned experiment, as shown in table 1 and Fig. 1, acted on the 3rd day in medicine, CORTEX AILANTHI group and control group phase
Than polypide quantity is largely reduced on fish, and killing rate is that 92% or so (P ＜ 0.01, P value are will to observe result in statistics to think
Effectively there is the probability of making a mistake of overall representative, 0.05 >=P ＞ 0.01 have statistical significance, and 0.01 >=P >=0.001 has
There is height statistical significance, on the contrary P values are bigger, then the variable in statistic processes is bigger to the influence degree of result), have extremely notable
Difference；Group play both sides of the street compared with control group, polypide quantity is largely reduced, killing rate is 80% or so (P ＜ 0.05), there is conspicuousness
Difference；Pumpkin subgroup is compared with control group, and there was no significant difference (P ＞ 0.05) for polypide quantity on fish；Compound Chinese medicine group is with compareing
Group is compared, and polypide quantity is largely reduced on fish, and killing rate is 87% or so (P ＜ 0.01), there is pole significant difference.
As shown in table 1 and Fig. 2, acted on the 8th day in medicine, CORTEX AILANTHI group is compared with control group, and polypide quantity is without aobvious on fish
Write sex differernce (P ＞ 0.05)；Group play both sides of the street compared with control group, polypide quantity is reduced seldom, killing rate is 62% or so (P ＜
0.05), there is significant difference；Pumpkin subgroup is compared with control group, and polypide quantity is greatly decreased on fish, and killing rate is 92% or so
(P ＜ 0.01), there is pole significant difference；Compound Chinese medicine group is compared with control group, and polypide quantity quantity minimize on fish, killing rate
For 97% or so (P ＜ 0.01), there is pole significant difference；It can be obtained by experimental data, the compound Chinese medicine is relative to single component pair
The killing action of Dactylogyrus adult and worm's ovum is more efficient.
Experiment two
Two experimental groups of this Setup Experiments, wherein experimental group A be the compound Chinese medicine composition to Dactylogyrus adult and
The effect of worm's ovum, experimental group B is the effect after the addition auxiliary material in compound Chinese medicine to Dactylogyrus adult and worm's ovum.
Experimental group A：CORTEX AILANTHI 1.25g is weighed respectively, the 1.25g and pumpkin seeds 2.5g that plays both sides of the street is obtained after crossing the mixing of 40 mesh sieves
To the Solid powder agent, the Solid powder agent is dissolved in 400mL water, by 60 DEG C of constant temperature 2h of thermograde, 70 DEG C of constant temperature
2h, 80 DEG C of constant temperature 2h stay overnight water extraction, remove and 100mL compound Chinese medicine A stostes are concentrated into after filter residue.
Experimental group B：CORTEX AILANTHI 1.25g, the 1.25g that plays both sides of the street, pumpkin seeds 2.5g, garlic powder 0.188g, matrimony vine are weighed respectively
0.125g, bark of eucommia 0.125g, Verbena officinalis 0.25g, radix scutellariae 0.125g, rhizoma zingiberis 0.25g, tuber of stemona 0.125g, kuh-seng 0.25g, pomegranate
Skin 0.125g, Paris polyphylla 0.125g and green bristlegrass 0.188g are crushed, and the Solid powder agent are obtained after crossing the mixing of 40 mesh sieves, by institute
State Solid powder agent to be dissolved in 400mL water, water extraction stayed overnight by 60 DEG C of constant temperature 2h of thermograde, 70 DEG C of constant temperature 2h, 80 DEG C of constant temperature 2h,
Remove and 100mL compound Chinese medicine B stostes are concentrated into after filter residue.
Added in aquarium after 100L water, aeration 8h, every group takes 10 goldfish temporarily to support after 24h, is separately added into compound Chinese medicine A
Stoste, compound Chinese medicine B stostes (blank control group is addition 100mL water), 25 DEG C of experimental temperature.
The combination drug of the combination drug of table 2 and addition auxiliary material acts on Dactylogyrus quantity after the 3rd day and the 8th day
By above-mentioned experiment, the raw material of the compound Chinese medicine A is CORTEX AILANTHI, play both sides of the street, pumpkin seeds are according to parts by weight
1:1:2 ratio is composited, as shown in Table 2 and Figure 3, is acted on the 3rd day in medicine, compound Chinese medicine A groups and blank control group phase
Than polypide quantity is greatly decreased on fish, and killing rate is 86% or so (P ＜ 0.01), there is pole significant difference；Compound Chinese medicine B be
The auxiliary materials such as garlic powder, matrimony vine, the bark of eucommia are added in compound Chinese medicine A to constitute, and as shown in Table 2 and Figure 3, are acted on the 3rd day in medicine, it is multiple
Chinese medicine B groups are closed compared with control group, polypide quantity is greatly decreased on fish, killing rate is 91% or so (P ＜ 0.01), there is extremely notable
Difference.
As shown in table 2 and Fig. 4, acted on the 8th day in medicine, compound Chinese medicine A groups are compared with blank control group, polypide on fish
Quantity is greatly decreased, and killing rate is 95% or so (P ＜ 0.01), there is pole significant difference；As shown in table 2 and Fig. 4, in medicine effect
8th day, compound Chinese medicine B groups can't check polypide compared with control group on fish, and (P ＜ 0.01) has pole significant difference.
The invention solve traditional national standard medicine to Dactylogyrus prevention effect worse and worse, drug abuse, harm aquatic products
The problem of product safe mass, reduction doctylogyrosis illness rate and the incidence of disease, improve breeding survival rate.The compound Chinese medicine solution of the present invention
Dactylogyrus of having determined can keep taking into account the Dactylogyrus forward and backward phase to the easy generation drug resistance problems of single medicine, and compound Chinese medicine
Killing action.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art
Realize other modification.Therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details and shown here as the legend with description.
Claims (8)
1. a kind of compound Chinese medicine for preventing and treating fish doctylogyrosis, wherein, mainly include the raw material of following parts by weight：CORTEX AILANTHI 10-
30 parts, play both sides of the street 10-30 parts and 10-70 parts of pumpkin seeds.
2. the compound Chinese medicine of preventing and treating fish doctylogyrosis as claimed in claim 1, wherein, in addition to following parts by weight is auxiliary
Material：1-5 parts of garlic powder, 1-3 parts of matrimony vine, 1-3 parts of the bark of eucommia, 1-7 parts of Verbena officinalis, 1-3 parts of radix scutellariae, 1-7 parts of rhizoma zingiberis, 1-3 parts of the tuber of stemona,
1-5 parts of 1-7 parts of kuh-seng, 1-3 parts of granatum, 1-3 parts of Paris polyphylla and green bristlegrass.
3. the compound Chinese medicine of fish doctylogyrosis is prevented and treated as claimed in claim 2, wherein, include the raw material of following parts by weight：
20 parts of CORTEX AILANTHI, play both sides of the street 20 parts, 40 parts of pumpkin seeds, 3 parts of garlic powder, 2 parts of matrimony vine, 2 parts of the bark of eucommia, 4 parts of Verbena officinalis, 2 parts of radix scutellariae,
3 parts of 4 parts of rhizoma zingiberis, 2 parts of the tuber of stemona, 4 parts of kuh-seng, 2 parts of granatum, 2 parts of Paris polyphylla and green bristlegrass.
4. a kind of preparation method of the compound Chinese medicine of preventing and treating fish doctylogyrosis as claimed in claim 2, wherein, including it is following
Step：
Step 1: by CORTEX AILANTHI, play both sides of the street, pumpkin seeds, garlic powder, matrimony vine, the bark of eucommia, Verbena officinalis, radix scutellariae, rhizoma zingiberis, the tuber of stemona, hardship
Ginseng, granatum, Paris polyphylla and green bristlegrass are crushed to more than 30 mesh standby respectively；
Step 2: the various raw materials that step one is crushed cross more than 30 mesh sieves respectively, well mixed that Solid powder agent is made is standby；
Step 3: the Solid powder agent is soluble in water, by the gradient increased temperature progress water extraction in the range of 50-100 DEG C, stay overnight simultaneously
The dregs of a decoction are filtered, the extract solution of the compound Chinese medicine of the preventing and treating fish doctylogyrosis is produced.
5. the preparation method of the compound Chinese medicine of fish doctylogyrosis is prevented and treated as claimed in claim 4, wherein, the step one
In, the CORTEX AILANTHI, play both sides of the street, pumpkin seeds, garlic powder, matrimony vine, the bark of eucommia, Verbena officinalis, radix scutellariae, rhizoma zingiberis, the tuber of stemona, kuh-seng, pomegranate
Skin, Paris polyphylla and green bristlegrass are fully dried before crushing.
6. the preparation method of the compound Chinese medicine of fish doctylogyrosis is prevented and treated as claimed in claim 4, wherein, the step 2
In, the various raw materials of the crushing cross that 40 mesh sieves are well mixed to be made Solid powder agent respectively.
7. the preparation method of the compound Chinese medicine of fish doctylogyrosis is prevented and treated as claimed in claim 4, wherein, the step 3
In, the thermograde is：60 DEG C of constant temperature 2h, 70 DEG C of constant temperature 2h, 80 DEG C of constant temperature 2h, the time overnight is minimum 6h.
8. a kind of application method of the compound Chinese medicine of preventing and treating fish doctylogyrosis as claimed in claim 2, wherein, will be described multiple
Conjunction Chinese medicine, which is put into breeding water body, to be used, and injected volume is using the final concentration of 40-100mg/L of the raw material as standard.
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| CN108159194A (en) * | 2017-12-08 | 2018-06-15 | 武汉中博水产生物技术有限公司 | A kind of Verbena officinalis preparation and its application for being used to prevent Dactylogyrus |
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2017
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