CN107058095A - Hand-held electroporation device - Google Patents
Hand-held electroporation device Download PDFInfo
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- CN107058095A CN107058095A CN201710071412.8A CN201710071412A CN107058095A CN 107058095 A CN107058095 A CN 107058095A CN 201710071412 A CN201710071412 A CN 201710071412A CN 107058095 A CN107058095 A CN 107058095A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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Abstract
The invention discloses a kind of hand-held electroporation device, including：Housing, housing is limited and electric pulse unit and sampling unit is sequentially provided with closed accommodating chamber, closed accommodating chamber, and housing is provided with mounting hole, and mounting hole is communicated with closed accommodating chamber；Pipette tips component, pipette tips component is connected with mounting hole, pipette tips component includes pipette tips, pipette tips limit liquid storage cylinder, and liquid storage cylinder is used to store the suspension containing cell and allogenic material, and liquid storage cylinder has gas inlet-outlet and liquid gateway, sampling unit is connected with gas inlet-outlet, pipette tips component is electrically connected with electric pulse unit, and the electric pulse produced by electric pulse unit causes liquid storage cylinder to produce electric field and carry out electroporation to cell so that allogenic material is directed into intracellular.Hand-held electroporation device according to embodiments of the present invention is easy to use and quickness and high efficiency.
Description
Technical field
The invention belongs to cell transfecting equipment technical field, in particular it relates to a kind of hand-held electroporation device.
Background technology
Traditional electroporation device is bulky, operation inconvenience, electroporation device and separate, the electroporation of electroporation cup
Equipment is not provided simultaneously with liquid relief and the function of electroporation.In electroporation experiment, it is necessary first to the cell mixing and outer in EP pipes
After the material of source, then the suspension containing cell and allogenic material is drawn in electroporation cup carries out electroporation, then will be through
The suspension crossed after electroporation is drawn in culture dish again, is operated extremely cumbersome.
The content of the invention
It is contemplated that at least solving one of above-mentioned technical problem to a certain extent.
Therefore, the present invention proposes a kind of hand-held electroporation device, the hand-held electroporation device is easy to operate and quick
Efficiently.
Hand-held electroporation device according to embodiments of the present invention includes：Housing, the housing limits closed accommodating chamber,
The closed electric pulse unit and sampling unit, the housing are provided with mounting hole, the mounting hole and the closed accommodating chamber phase
Even；Pipette tips component, the pipette tips component is connected with the mounting hole, and the pipette tips component includes pipette tips, and the pipette tips are limited
Liquid storage cylinder, the liquid storage cylinder is used to store the suspension containing cell and allogenic material, and the liquid storage cylinder has gas inlet-outlet
With liquid gateway, the sampling unit is connected with the gas inlet-outlet, the pipette tips component and electric pulse unit electricity
Connection, the electric pulse produced by the electric pulse unit causes liquid storage cylinder to produce electric field and carry out electroporation to cell so that external source
Material is directed into intracellular.
Hand-held electroporation device according to embodiments of the present invention, by the way that electric pulse unit and sampling unit is integrally disposed
In in closed accommodating chamber, electroporation is directly carried out to the cell suspending liquid in liquid storage cylinder so as to realize, compared to traditional electricity
For perforating apparatus, easy to use and quickness and high efficiency.
In addition, hand-held electroporation device according to embodiments of the present invention, can also have technical characteristic additional as follows：
According to one embodiment of present invention, the hand-held electroporation device also includes：Control unit, the control list
Member includes：Control board and display device, the control board be arranged in the closed accommodating chamber and respectively with it is described
Electric pulse unit and sampling unit connection, the display device are arranged on the side wall of the housing and the control circuit
Plate is electrically connected.
According to one embodiment of present invention, the hand-held electroporation device also includes：Power supply unit, the power supply list
Member is located in the closed accommodating chamber, said supply unit respectively with described control unit, the sampling unit and the electric arteries and veins
Rush unit electrical connection.
According to one embodiment of present invention, the pipette tips component also includes：Assembly connection body, the assembly connection body
One end is embedded in the mounting hole, and the other end of the assembly connection body stretches out the mounting hole, and the assembly connection body is provided with
Two electrode needles electrically connected with the electric pulse unit, the pipette tips are connected with the other end of the assembly connection body, described
Pipette tips are provided with two conductive plates being oppositely arranged, and two electrode needles are electrically connected with two facing conductive plates respectively.
According to one embodiment of present invention, the pipette tips and the assembly connection body are detachably connected.
According to one embodiment of present invention, insulating barrier is provided between two conductive plates, the insulating barrier is limited
Liquid storage cylinder, the conductive plate is fitted in the lateral wall of the insulating barrier.
According to one embodiment of present invention, the insulating barrier includes：Tube body, one end of the tube body and the dress
Other end connection with connector；Cavity portion, the cavity portion is connected with the other end of the tube body, and the cavity portion is substantially
For inverted-triangular.
According to one embodiment of present invention, the hand-held electroporation device also includes：Sample plug bar assembly, it is described enter
Sample stopper rod component includes：Circle hole groove, the circle hole groove and the closed accommodating chamber are provided with sample introduction stopper rod, the closed accommodating chamber
Connection, one end of the sample introduction stopper rod is stretched into the circle hole groove, and the other end of the sample introduction stopper rod is located at outside the circle hole groove,
The sample introduction stopper rod is movably arranged in the presence of outer power in the circle hole groove；Elastic component, the elastic component is located at described
In circle hole groove, one end of the sample introduction stopper rod, which is stopped, is butted on the elastic component.
According to one embodiment of present invention, the perisporium of the circle hole groove by the closed accommodating chamber be separated into upper chamber and
Lower chambers, said supply unit and described control unit are respectively provided in the upper chamber, sampling unit and the electric pulse list
Member is respectively provided at the lower chambers.
According to one embodiment of present invention, the housing includes：First housing and the second housing, first housing and
The second shell body is oppositely arranged structure and sets out the closed accommodating chamber.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Brief description of the drawings
The above-mentioned and/or additional aspect and advantage of the present invention will become from description of the accompanying drawings below to embodiment is combined
Substantially and be readily appreciated that, wherein：
Fig. 1 is the stereogram of hand-held electroporation device according to an embodiment of the invention；
Fig. 2 is the exploded view of hand-held electroporation device according to an embodiment of the invention；
Fig. 3 is the exploded view of pipette tips according to an embodiment of the invention；
Fig. 4 is the workflow diagram of hand-held electroporation device according to an embodiment of the invention.
Embodiment
Embodiments of the invention are described below in detail, the example of the embodiment is shown in the drawings, wherein from beginning to end
Same or similar label represents same or similar element or the element with same or like function.Below with reference to attached
The embodiment of figure description is exemplary, it is intended to for explaining the present invention, and be not considered as limiting the invention.
Hand-held electroporation device 100 according to embodiments of the present invention is described referring to Fig. 1-Fig. 3.The hand-held electricity is worn
Hole can substantially include：Housing 10 and pipette tips component 40.
Specifically, as shown in Fig. 2 housing 10, which is limited in closed accommodating chamber, closed accommodating chamber, is sequentially provided with electric pulse list
Member 20 and sampling unit 30, housing 10 are provided with mounting hole 11, and mounting hole 11 is communicated with closed accommodating chamber.That is, this is hand-held
The electric pulse unit 20 and sampling unit 30 of formula electroporation device 100 are integrally disposed in the closed accommodating chamber of housing 10.By
This so that hand-held electroporation device 100 is provided simultaneously with the function of sampling and electroporation.
Reference picture 1 is with reference to shown in Fig. 3, and pipette tips component 40 is connected with mounting hole 11, and pipette tips component 40 includes pipette tips 41, pipette tips
41 limit liquid storage cylinder 411, and liquid storage cylinder 411 is used to store the suspension containing cell and allogenic material, and liquid storage cylinder 411 has gas
Body gateway 4111 and liquid gateway 4112, sampling unit 30 are connected with gas inlet-outlet 4111, pipette tips component 40 and electric arteries and veins
Rush unit 20 to electrically connect, the electric pulse produced by electric pulse unit 20 causes liquid storage cylinder 411 to produce electric field to carry out electricity to cell
Perforation, and cause allogenic material is directed into intracellular.
Shown in reference picture 4, hand-held electroporation device 100 generallys include following steps to cell transfecting：S100, which takes, to be waited to turn
Cell is contaminated to liquid storage cylinder 411：Sampling unit 30 is aspirated to liquid storage cylinder 411 so that the gas in liquid storage cylinder 411 goes out from gas
Entrance 4111 is discharged and forms negative pressure, and the suspension containing cell and allogenic material is from liquid gateway 4112 under suction function
It is sucked into liquid storage cylinder 411；S200 electroporations cell to be transfected：Electric pulse produced by electric pulse unit 20 causes liquid storage cylinder
411 produce electric field to carry out electroporation to cell, and cause allogenic material is directed into intracellular；S300 transfers are by electroporation
Cell suspending liquid：Air is passed through to liquid storage cylinder 411 by sampling unit 30, gas enters liquid storage cylinder from gas inlet-outlet 4111
Cell suspending liquid is discharged from liquid gateway 4112 in 411, and be transferred in culture dish and cultivated.
It is understood that presetting the relevant parameter of electric pulse unit 20 before sampling：The class of such as voltage pulse
Type, voltage amplitude, the width of each pulse, continuous impulse number, the interval of two pulses, meanwhile, preset sampling unit
30 sampling volume.
Hand-held electroporation device 100 according to embodiments of the present invention, by by electric pulse unit 20 and sampling unit 30
It is integrally disposed in closed accommodating chamber, electroporation, phase are directly carried out to the cell suspending liquid in liquid storage cylinder 411 so as to realize
Than for conventional electroporation equipment, easy to use and quickness and high efficiency.
In some embodiments of the invention, as depicted in figs. 1 and 2, housing 10 includes：First housing 13 and the second housing
14, the first housing 13 and the second housing 14 are oppositely arranged structure and set out closed accommodating chamber.Wherein, the first housing 13 and the second housing 14
It is detachably connected, for example, can be connected with the first housing 13 and the second housing 14 by screw or snap.Thus,
Simplify the manufacturing process of hand-held electroporation device 100, reduce production cost.
Alternatively, hand-held electroporation device 100 also includes：Control unit 50.Control unit 50 includes：Control board
51 and display device 52.Control board 51 be arranged in closed accommodating chamber and respectively with electric pulse unit 20 and sampling unit 30
Connection, display device 52 is arranged on the side wall of housing 10 and electrically connected with control board 51.
That is, the type of the voltage pulse of electric pulse unit 20, voltage amplitude, the width of each pulse, continuous arteries and veins
Rushing the interval of number and two pulses can be controlled by control board 51, and the sample volume of sampling unit 30 also may be used
To be controlled by control board 51.And the parameter of all settings can be shown by display device 52.Thus, enter
One step is easy to operator to set different parameters for different cell categories, the need for meeting different experiments requirement, so as to improve
The versatility of hand-held electroporation device 100.
Advantageously, can be provided with the adjusting knob 21 for adjusting the relevant parameter of electric pulse unit 20 and for performing on housing 10
The button 31 of the sampling action of sampling unit 30, thus, further improves the convenience of the operation of hand-held electroporation device 100.
Alternatively, hand-held electroporation device 100 can also include：Power supply unit 60.Power supply unit 60 is located at closed appearance
Receive intracavitary, power supply unit 60 is electrically connected with control unit 50, sampling unit 30 and electric pulse unit 20 respectively.That is, for
Electric unit 60 can provide power supply to control unit 50, sampling unit 30 and electric pulse unit 20 simultaneously.Thus, it is possible to so that whole
The design of individual hand-held electroporation device 100 is more reasonable, meets operator and uses hand-held electroporation device in different occasions
The need for 100.
In other embodiments of the invention, as shown in Figure 1 with Figure 2, pipette tips component 40 also includes：Assembly connection body
42.In one end insertion mounting hole 11 of assembly connection body 42, the other end of assembly connection body 42 stretches out mounting hole 11, assembly connection
Body 42 is provided with two electrode needles 43 electrically connected with electric pulse unit 20, and pipette tips 41 are connected with the other end of assembly connection body 42,
Pipette tips 41 are provided with two conductive plates 412 being oppositely arranged, and two electrode needles 43 are electrically connected with two conductive plates 412 respectively.Change speech
It, one end of two electrode needles 43 is electrically connected with two conductive plates 412, the other end and the electric pulse unit electricity of two electrode needles 43
20 connections, the impulse electric field produced by electric pulse unit 20 can be transferred to conductive plate 412 by two electrode needles 43, so that
Impulse electric field is formed between the conductive plate 412 that two are oppositely arranged, and then electricity can be carried out to the cell in liquid storage cylinder 411 and is worn
Hole.
Alternatively, the lateral wall that two electrode needles 43 can be respectively with two conductive plates 412 is connected, two electrode needles 43 it
Between spacing d1The distance between generally two conductive plates 412 d2。
Alternatively, the spacing d of two electrode needles 431Can be between 0.2-2 millimeters.Preferably, d1For 1 millimeter.
In some embodiments, pipette tips 41 and assembly connection body 42 are detachably connected.So, complete electroporation it
Afterwards, pipette tips 41 from assembly connection body 42 can be taken off cleaning, or uses disposable pipette tips 41, after each electroporation terminates
Change and use new pipette tips 41.Thus, it is possible to cross pollution occur during avoiding cell transfecting.
In one particular embodiment of the present invention, reference picture 2 is with reference to shown in Fig. 3, provided with exhausted between two conductive plates 412
Edge layer 413, insulating barrier 413 limits liquid storage cylinder 411, and conductive plate 412 is fitted in the lateral wall of insulating barrier 413.In other words, insulate
Layer 413 is folded between two conductive plates 412, so that stable impulse electric field can be formed between two conductive plates 412,
Electroporation effectively is carried out to the cell in liquid storage cylinder 411.
Further, insulating barrier 413 includes：Tube body 4131 and cavity portion 4132, one end of tube body 4131 and assembling
The other end connection of connector 42.Cavity portion 4132 is connected with the other end of tube body 4131, cavity portion 4132 generally fall three
Angle-style.As shown in figure 3, the shape and structure of two conductive plates 412 and insulating barrier 413 is adapted, two conductive plates 412 are coated on absolutely
The outside of edge layer 413.Wherein, tack coat can be provided between insulating barrier 413 and conductive plate 412, passes through tack coat reinforced insulation
Bonding strength between layer 413 and conductive plate 412.
In still other embodiments of the present invention, hand-held electroporation device 100 can also include：Sample plug bar assembly 70.
Sample plug bar assembly 70 includes：Sample introduction stopper rod 71 and elastic component 72.As shown in Fig. 2 circle hole groove 12 is provided with closed accommodating chamber, circle
Hole slot 12 is connected with closed accommodating chamber, and one end of sample introduction stopper rod 71 is stretched into circle hole groove 12, and the other end of sample introduction stopper rod 71 is located at
Outside circle hole groove 12, sample introduction stopper rod 71 is movably arranged in the presence of external force in circle hole groove 12.Elastic component 72 is located at circle hole groove
In 12, one end of sample introduction stopper rod 71, which is stopped, is butted on elastic component 72.
Alternatively, the installation through-hole of hand-held electroporation device 100 can be provided with pipette, hand-held electroporation device
100 have the function of common liquid-transfering gun.By press sample introduction stopper rod 71 so that sample introduction stopper rod 71 from the other end (sample introduction stopper rod 71
Outer end) moved to one end (the inner of sample introduction stopper rod 71) direction, deformed upon after the stress of elastic component 72, gas in circle hole groove 12
Flowed to closed accommodating chamber, force the gas in pipette to be discharged, after the external force suffered by sample introduction stopper rod 71 disappears, elasticity
The air pressure reduction for causing closed accommodating chamber, liquid are moved in direction to the restoring force driving sample introduction stopper rod 71 of part 72 from one end to the other side
It is sucked into pipette, when pressing sample introduction stopper rod 71 again, the liquid in pipette is discharged, so that hand-held electricity is worn
Aperture apparatus 100 completes the process of imbibition and discharge opeing.
Wherein, closed accommodating chamber can be separated into upper chamber and lower chambers, the He of power supply unit 60 by the perisporium of circle hole groove 12
Control unit 50 is located in upper chamber, and sampling unit and electric pulse unit 20 are located at lower chambers.Thus, it is possible to improve hand-held electricity
The reasonability of the structure design of punching machine 100, it is ensured that the sealing of closed accommodating chamber, improves sample introduction precision.
In the description of the invention, it is to be understood that the side of the instruction such as term " on ", " under " ", bottom ", " interior ", " outer "
Position or position relationship are, based on orientation shown in the drawings or position relationship, to be for only for ease of the description present invention and simplify description,
Rather than indicate or imply that signified device or element must have specific orientation, with specific azimuth configuration and operation, because
This is not considered as limiting the invention.
In the present invention, unless otherwise clearly defined and limited, term " installation ", " connected ", " connection ", " fixation " etc.
Term should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected, or be integrally connected；It can be machine
Tool is connected or electrically connected；Can be joined directly together, can also be indirectly connected to by intermediary, can be two members
Connection inside part.For the ordinary skill in the art, above-mentioned term can be understood in this hair as the case may be
Concrete meaning in bright.
In the present invention, unless otherwise clearly defined and limited, fisrt feature second feature it " on " or it " under "
Can directly it be contacted including the first and second features, it is not direct contact but by it that can also include the first and second features
Between other characterisation contact.Moreover, fisrt feature second feature " on ", " top " and " above " to include first special
Levy directly over second feature and oblique upper, or be merely representative of fisrt feature level height higher than second feature.Fisrt feature exists
Second feature " under ", " lower section " and " following " fisrt feature that includes are directly over second feature and oblique upper, or be merely representative of
Fisrt feature level height is less than second feature.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means to combine specific features, structure, material or the spy that the embodiment or example are described
Point is contained at least one embodiment of the present invention or example.In this manual, to the schematic representation of above-mentioned term not
Necessarily refer to identical embodiment or example.Moreover, specific features, structure, material or the feature of description can be any
One or more embodiments or example in combine in an appropriate manner.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art is not departing from the principle and objective of the present invention
In the case of above-described embodiment can be changed within the scope of the invention, change, replace and modification.
Claims (10)
1. a kind of hand-held electroporation device, it is characterised in that including：
Housing, the housing, which is limited, is sequentially provided with electric pulse unit and sampling list in closed accommodating chamber, the closed accommodating chamber
Member, the housing is provided with mounting hole, and the mounting hole is communicated with the closed accommodating chamber；
Pipette tips component, the pipette tips component is connected with the mounting hole, and the pipette tips component includes pipette tips, and the pipette tips are limited
Liquid storage cylinder, the liquid storage cylinder is used to store the suspension containing cell and allogenic material, and the liquid storage cylinder has gas inlet-outlet
With liquid gateway, the sampling unit is connected with the gas inlet-outlet, the pipette tips component and electric pulse unit electricity
Connection, the electric pulse produced by the electric pulse unit to produce electric field in liquid storage cylinder to carry out electroporation to cell, and makes
Allogenic material be directed into it is intracellular.
2. hand-held electroporation device according to claim 1, it is characterised in that also include：Control unit, the control
Unit includes：Control board and display device, the control board be arranged in the closed accommodating chamber and respectively with institute
Electric pulse unit and sampling unit connection are stated, the display device is arranged on the side wall of the housing and the control electricity
Road plate electrical connection.
3. hand-held electroporation device according to claim 2, it is characterised in that also include：Power supply unit, the power supply
Unit is located in the closed accommodating chamber, said supply unit respectively with described control unit, the sampling unit and the electricity
Pulse unit is electrically connected.
4. hand-held electroporation device according to claim 1, it is characterised in that the pipette tips component also includes：
Assembly connection body, one end of the assembly connection body is embedded in the mounting hole, and the other end of the assembly connection body is stretched
Go out the mounting hole, the assembly connection body is provided with two electrode needles electrically connect with the electric pulse unit, the pipette tips and
The other end connection of the assembly connection body, the pipette tips are provided with two conductive plates being oppositely arranged, two electrode needles point
Do not electrically connected with two conductive plates.
5. hand-held electroporation device according to claim 4, it is characterised in that the pipette tips and the assembly connection body
It is detachably connected.
6. hand-held electroporation device according to claim 4, it is characterised in that provided with exhausted between two conductive plates
Edge layer, the insulating barrier limits liquid storage cylinder, and the conductive plate is fitted in the lateral wall of the insulating barrier.
7. hand-held electroporation device according to claim 6, it is characterised in that the insulating barrier includes：
Tube body, one end of the tube body is connected with the other end of the assembly connection body；
Cavity portion, the cavity portion is connected with the other end of the tube body, the cavity portion generally inverted-triangular.
8. hand-held electroporation device according to claim 4, it is characterised in that also include：Sample plug bar assembly,
The sample plug bar assembly includes：
Sample introduction stopper rod, circle hole groove is provided with the closed accommodating chamber, and the circle hole groove is connected with the closed accommodating chamber, it is described enter
One end of sample stopper rod is stretched into the circle hole groove, and the other end of the sample introduction stopper rod is located at outside the circle hole groove, the sample plug
Bar is movably arranged in the presence of external force in the circle hole groove；
Elastic component, the elastic component is located in the circle hole groove, and one end of the sample introduction stopper rod, which is stopped, is butted on the elastic component.
9. hand-held electroporation device according to claim 8, it is characterised in that the perisporium of the circle hole groove will be described close
Close accommodating chamber and be separated into upper chamber and lower chambers, said supply unit and described control unit are set respectively in the upper chamber,
Sampling unit and the electric pulse unit is respectively provided at the lower chambers.
10. hand-held electroporation device according to claim 1, it is characterised in that the housing includes：First housing and
Second housing, first housing and the second shell body are oppositely arranged structure and set out the closed accommodating chamber.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710071412.8A CN107058095B (en) | 2017-02-09 | 2017-02-09 | Hand-held electroporation device |
| PCT/CN2017/110498 WO2018145495A1 (en) | 2017-02-09 | 2017-11-10 | Handheld electric drilling apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710071412.8A CN107058095B (en) | 2017-02-09 | 2017-02-09 | Hand-held electroporation device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107058095A true CN107058095A (en) | 2017-08-18 |
| CN107058095B CN107058095B (en) | 2020-07-28 |
Family
ID=59599239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710071412.8A Active CN107058095B (en) | 2017-02-09 | 2017-02-09 | Hand-held electroporation device |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN107058095B (en) |
| WO (1) | WO2018145495A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018145495A1 (en) * | 2017-02-09 | 2018-08-16 | 清华大学 | Handheld electric drilling apparatus |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101426929A (en) * | 2004-05-12 | 2009-05-06 | 麦克赛特股份有限公司 | Methods and devices related to a regulated flow electroporation chamber |
| CN102341492A (en) * | 2008-04-15 | 2012-02-01 | 英杰生命新加坡私人有限公司 | Pipette tip for electroporation device |
| WO2013166203A2 (en) * | 2012-05-01 | 2013-11-07 | Life Technologies Corporation | Pippette devices, systems, and methods of use thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5318514A (en) * | 1992-08-17 | 1994-06-07 | Btx, Inc. | Applicator for the electroporation of drugs and genes into surface cells |
| US20050282283A1 (en) * | 2004-04-19 | 2005-12-22 | Laura Vozza-Brown | Electroporation apparatus and methods |
| EP1766004B1 (en) * | 2004-06-12 | 2016-08-31 | Invitrogen Singapore Pte. Ltd. | Electroporator having an elongated hollow member |
| WO2014127510A1 (en) * | 2013-02-20 | 2014-08-28 | Jian Chen | Methods and devices for electroporation |
| CN103937669B (en) * | 2014-03-18 | 2016-04-06 | 苏州壹达生物科技有限公司 | A kind of hand-held electroporation device |
| CN107058095B (en) * | 2017-02-09 | 2020-07-28 | 清华大学 | Hand-held electroporation device |
-
2017
- 2017-02-09 CN CN201710071412.8A patent/CN107058095B/en active Active
- 2017-11-10 WO PCT/CN2017/110498 patent/WO2018145495A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101426929A (en) * | 2004-05-12 | 2009-05-06 | 麦克赛特股份有限公司 | Methods and devices related to a regulated flow electroporation chamber |
| CN102341492A (en) * | 2008-04-15 | 2012-02-01 | 英杰生命新加坡私人有限公司 | Pipette tip for electroporation device |
| WO2013166203A2 (en) * | 2012-05-01 | 2013-11-07 | Life Technologies Corporation | Pippette devices, systems, and methods of use thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018145495A1 (en) * | 2017-02-09 | 2018-08-16 | 清华大学 | Handheld electric drilling apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2018145495A1 (en) | 2018-08-16 |
| CN107058095B (en) | 2020-07-28 |
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