CN107056961A - Pulullan polysaccharide purifying technique - Google Patents
Pulullan polysaccharide purifying technique Download PDFInfo
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- CN107056961A CN107056961A CN201611267725.2A CN201611267725A CN107056961A CN 107056961 A CN107056961 A CN 107056961A CN 201611267725 A CN201611267725 A CN 201611267725A CN 107056961 A CN107056961 A CN 107056961A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
- C08B37/0018—Pullulan, i.e. (alpha-1,4)(alpha-1,6)-D-glucan; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12P19/10—Pullulan
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Abstract
Enter plate-frame filtering after decolouring the present invention relates to the zymotic fluid of polysaccharide production method, i.e. pulullan polysaccharide purifying technique, including removing thalline, enter back into crystallizer, stream plus alcohol are crystallized, and crystal solution is squeezed into seperator carries out isolated wet product, is characterized in：Wet product is dissolved in water, and regulation pH value is crossed after film desalination, desalination into ultrafiltration circulating tank and adds to lysate into crystallizing tank, stream plus alcohol are recrystallized, crystal solution is centrifuged, and obtain wet feed, and fine work is dried to obtain in progress to 5.5 6.5.Beneficial effect is：Pulullan polysaccharide purity brings up to more than 99% by 95%, and whiteness brings up to more than 90% by 85%, and light transmittance brings up to more than 90% by 80%, and engineering is simple, with low cost, and added value of product and remarkable in economical benefits are improved.
Description
Technical field
The present invention relates to polysaccharide production method, i.e. pulullan polysaccharide purifying technique.
Background technology
Pulullan polysaccharide is by the similar glucan produced by Aureobasidium pullulans fermentation, the extracellular water-soluble cement of xanthans
Polysaccharide, it is the special microbial polysaccharide found by R.Bauer in 1938.The polysaccharide is by the wheat of α-Isosorbide-5-Nitrae glycosidic bond connection
The straight-chain polysaccharide that bud trisaccharide recurring unit is polymerized through α -1,6 glycosidic bonds, molecular weight 20,000 ~ 2,000,000, the degree of polymerization 100 ~
5000.(general merchandise molecular weight is about made up of in 200,000 or so 480 maltotrioses).The polysaccharide has two important spies
Property:High resilience in structure, solubility is than larger.The film forming of pulullan polysaccharide, gas barrier property, plasticity, viscosity are relatively strong, and
And with the good characteristic such as soluble in water, nontoxic, colorless and odorless, be widely used to medicine, food, light industry, chemical industry and
The fields such as oil.On May 19th, 2006, health ministries issued No. 8 bulletin, and pulullan polysaccharide is to increase four kinds of food additives newly
One of plus agent product, fruit glaze agent and increasing can be used as in candy, chocolate-coated, diaphragm, complex flavor section and fruit-vegetable juice beverage
Thick dose.
Pulullan polysaccharide is water solubility cement polysaccharide, and finished product is white solid powder.Due to its have good film forming, into
The characteristics such as fiber, choke, bonding, easy processing, nontoxicity, are widely used to the neck such as medicine, food, light industry, chemical industry and oil
Domain.Pulullan polysaccharide main application fields:(1) medicine and health product capsule industry, the bonding binder of cosmetics.(2) food
The modifying agent and thickener of quality.(3) it is used for the water miscible packaging material of anti-oxidation.(4) the low calorie food of staple food, cake
Product raw material.
But the extracellular water solubility cement polysaccharide yield of the similar glucan, xanthans produced by Aureobasidium pullulans fermentation is simultaneously
It is not high.
The content of the invention
It is an object of the invention to provide can significantly improve pulullan yield and purity, and technique is simple, and cost is low
Honest and clean pulullan polysaccharide purifying technique.
Above-mentioned purpose is realized by following technical scheme：Pulullan polysaccharide purifying technique, including pulullan polysaccharide are provided
Purifying technique, its feature comprises the following steps：Aureobasidium pullulans removes the fermentation of thalline with being fermented in fermentation medium after fermentation
Liquid enters plate-frame filtering after decolouring, enters back into crystallizer, and stream plus alcohol are crystallized, and crystal solution is squeezed into seperator is carried out
Isolated wet product, the zymotic fluid of the removing thalline first passes through Medical stone filtering layer or quartz sand mistake before being decolourized
Filtering layer or expanded perlite filter layer or medical stone, quartz sand and expanded perlite mixing constitute filter layer；The fermented and cultured
Base is made up of following composition and its components by weight percent：Sucrose 6-16%；Yeast extract 0.1-O.5%；Epicatechol 0.01-0.1%, NaCl
0.1-1%；Ergosterol 0.01-0.1%, MgSO4-7H2O 0.01-0.1%；Butanedioic acid 0.1-1%, K2HPO4·3H2O 0.01-
0.1%；(NH4)2SO40.01-0.1% and surplus sterilized water.
The zymotic fluid of thalline is removed before being decolourized, Medical stone filtering layer is first passed through.
The particle diameter of medical stone granule is 1-2mm, and the filtering 200-300mm of thickness constituted, Medical stone filtering layer is located at
In filtering tank, filtration temperature is in 70-80 DEG C, pressure 0.5-0.8MPa.
The zymotic fluid of thalline is removed before being decolourized, quartz sand filtration layer is first passed through.
The particle diameter of quartz sand is 0.5-0.8mm, and the filtering 100-150mm of thickness constituted, filter layer is located at filtering tank
Interior, filtration temperature is in 70-80 DEG C, pressure 0.8-1MPa.
The zymotic fluid of thalline is removed before being decolourized, expanded perlite filter layer is first passed through.
The particle diameter of expanded perlite is 1.5-2.5mm, and the filtering 200-300mm of thickness constituted, filter layer was located at
In filter tank, filtration temperature is in 70-80 DEG C, pressure 0.8-1MPa.
The zymotic fluid of thalline is removed before being decolourized, medical stone, quartz sand and expanded perlite mixing structure is first passed through
Into filter layer.
The particle diameter of medical stone granule is 1-2mm, and the particle diameter of quartz sand is 0.5-0.8mm, and the particle diameter of expanded perlite is
1.5-2.5mm, its volume ratio is medical stone：Quartz sand：Expanded perlite=3-5:2—3:5-8, the filter layer constituted
200-300mm of thickness, filter layer is located in filtering tank, and filtration temperature is in 70-80 DEG C, pressure 0.8-1MPa.
The beneficial effects of the invention are as follows：Pulullan polysaccharide purity brings up to more than 99% by 95%, and whiteness is improved by 85%
To more than 90%, light transmittance brings up to more than 90% by 80%, and engineering is simple, with low cost, added value of product and economic effect
Benefit is significantly improved.
Embodiment
Embodiment 1：
Zymotic fluid is handled according to the following steps：
1st, decolourize：Zymotic fluid removes thalline, and regulation pH value is to 5.5-6.5, about 80 DEG C of temperature, and appropriate activity is added into zymotic fluid
Charcoal, insulation decolouring 20-30 minutes.
2nd, filter：Material is through plate-frame filtering.
3rd, crystallize：Material adds alcohol to be crystallized into crystallizing tank stream.
4th, separate：Above-mentioned crystal solution is centrifuged, wet product is obtained.
5th, dissolve：Crystallization wet product is dissolved in water.
6th, ultrafiltration：The hydrating solution for crystallizing wet product is adjusted into pH value to 5.5-6.5, film desalination is crossed into ultrafiltration circulating tank.
7th, recrystallize：Lysate is added into crystallizing tank, stream plus alcohol, material is crystallized again.
8th, separate：Crystal solution is centrifuged, and obtains wet product.
9th, dry：Wet feed is carried out to dry to obtain fine work.
Experimental conditions：Former technique and this technique are respectively produced five batches under the same conditions, data measured is drawn a conclusion：
The product quality of secondary crystallization is significantly improved.
Embodiment 2：
Zymotic fluid is handled according to the following steps：
1st, initial filter：Zymotic fluid removes thalline, by medical stone granule filter layer.The particle diameter of medical stone granule is 0.8-1.2mm,
Filtering 200-the 300mm of thickness constituted, Medical stone filtering layer is located in filtering tank, and filtration temperature is in 70-80, pressure 0.5-
0.8MPa。
2nd, decolourize：Gained filtrate adjusts pH value to 5.5-6.5, and about 80 DEG C of temperature adds proper amount of active carbon into zymotic fluid,
It is incubated decolouring 20-30 minutes.
3rd, filter：Material is through plate-frame filtering.
4th, crystallize：Material adds alcohol to be crystallized into crystallizing tank stream.
5th, separate：Above-mentioned crystal solution is centrifuged, wet product is obtained.
6th, dissolve：Crystallization wet product is dissolved in water.
7th, ultrafiltration：The hydrating solution for crystallizing wet product is adjusted into pH value to 5.5-6.5, film desalination is crossed into ultrafiltration circulating tank.
8th, recrystallize：Lysate is added into crystallizing tank, stream plus alcohol, material is crystallized again.
9th, separate：Crystal solution is centrifuged, and obtains wet product.
10th, dry：Wet product is carried out to dry to obtain fine work.
Fermentation medium is made up of following composition and its components by weight percent：Sucrose 6-16%；Yeast extract 0.1-O.5%；Table catechu
Phenol 0.01-0.1%, NaCl 0.1-1%；Ergosterol 0.01-0.1%, MgSO4-7H2O 0.01-0.1%；Butanedioic acid 0.1-1%,
K2HPO4·3H2O 0.01-0.1%；(NH4)2SO40.01-0.1% and surplus sterilized water, epicatechol and ergosterol pass through
Butanedioic acid and K2HPO43H2O can promote fermentative microorganism polysaccharide, so as to improve polysaccharide yield, medical stone granule here is put
Put in filtering tank, the screen cloth below provided with 0.5-0.7mm of aperture, supported again by aperture 50mm mesh floor below.Wheat
Meal stone particle can be cleared up by periodically recoiling, and can also regularly replace can after filtrate, used medical stone granule purified treatment
Recycling.
Experiment is proved, before using activated carbon decolorizing, is first passed through the initial filter of medical stone granule, can be significantly improved product
Its reason of purity is that medical stone granule has very big specific surface area, can both filter out a large amount of impurity, and adsorbable a large amount of pigments.
And the activated carbon dosage of bleaching process is greatly decreased, reduce production cost.Pulullan polysaccharide purity is improved by 95%
To more than 99%, whiteness brings up to more than 94% by 85%, and light transmittance brings up to more than 95% by 80%.
Embodiment 3：
Zymotic fluid is handled according to the following steps：
1st, initial filter：Zymotic fluid removes thalline, by quartz sand filtration layer.The particle diameter of quartz sand is 0.3-0.5mm, is constituted
100-150mm of thickness is filtered, filter layer is located in filtering tank, and filtration temperature is in 70-80, pressure 0.8-1MPa.
2nd, decolourize：Gained filtrate adjusts pH value to 5.5-6.5, and about 80 DEG C of temperature adds proper amount of active carbon into zymotic fluid,
It is incubated decolouring 20-30 minutes.
3rd, filter：Material is through plate-frame filtering.
4th, crystallize：Material adds alcohol to be crystallized into crystallizing tank stream.
5th, separate：Above-mentioned crystal solution is centrifuged, wet product is obtained.
6th, dissolve：Crystallization wet product is dissolved in water.
7th, ultrafiltration：The hydrating solution for crystallizing wet product is adjusted into pH value to 5.5-6.5, film desalination is crossed into ultrafiltration circulating tank.
8th, recrystallize：Lysate is added into crystallizing tank, stream plus alcohol, material is crystallized again.
9th, separate：Crystal solution is centrifuged, and obtains wet product.
10th, dry：Wet product is carried out to dry to obtain fine work.
Here quartz sand is put in filtering tank, the screen cloth below provided with aperture 0.2mm, below again by aperture 50mm
Mesh floor support.Quartz sand particle can be cleared up by periodically recoiling, and can also regularly replace filtrate, used quartz sand
Repeat and utilize after purified treatment.
Experiment is proved, before using activated carbon decolorizing, first passes through the initial filter of quartz sand particle, can both have been filtered out a large amount of miscellaneous
The consumption of activated carbon is greatly decreased in matter, and adsorbable a large amount of pigments, the purity that can significantly improve product, and reduction production cost is general
Shandong orchid purity of polysaccharide brings up to more than 99% by 95%, and whiteness brings up to more than 94% by 85%, and light transmittance is brought up to by 80%
More than 95%.
Embodiment 4：
Zymotic fluid is handled according to the following steps：
1st, initial filter：Zymotic fluid removes thalline, by expanded perlite filter layer.The particle diameter of expanded perlite is 1.5-2.5mm,
Filtering 200-the 300mm of thickness constituted, filter layer is located in filtering tank, and filtration temperature is in 70-80 DEG C, pressure 0.8-
1MPa。
2. decolourize：Gained filtrate adjusts pH value to 5.5-6.5, and about 80 DEG C of temperature adds proper amount of active carbon into zymotic fluid,
It is incubated decolouring 20-30 minutes.
3rd, filter：Material is through plate-frame filtering.
4th, crystallize：Material adds alcohol to be crystallized into crystallizing tank stream.
5th, separate：Above-mentioned crystal solution is centrifuged, wet product is obtained.
6th, dissolve：Crystallization wet product is dissolved in water.
7th, ultrafiltration：The hydrating solution for crystallizing wet product is adjusted into pH value to 5.5-6.5, film desalination is crossed into ultrafiltration circulating tank.
8th, recrystallize：Lysate is added into crystallizing tank, stream plus alcohol, material is crystallized again.
9th, separate：Crystal solution is centrifuged, and obtains wet product.
10th, dry：Wet product is carried out to dry to obtain fine work.
Here expanded perlite is placed in filtering tank, the screen cloth below provided with 0.8-1mm of aperture, below again by
Aperture 50mm mesh floor support.Expanded perlite granule can be cleared up by periodically recoiling, and can also regularly replace filtrate,
Reused after used expanded perlite purified treatment.
Experiment is proved, before using activated carbon decolorizing, first passes through expanded perlite granule initial filter, can both have been filtered out a large amount of miscellaneous
Matter, and adsorbable a large amount of pigments, can significantly improve the purity of product.The consumption of activated carbon can be greatly decreased, production cost is reduced.
Pulullan polysaccharide purity brings up to more than 99% by 95%, and whiteness brings up to more than 94% by 85%, light transmittance by
80% brings up to more than 95%.
Embodiment 5：
Zymotic fluid is handled according to the following steps：
1st, initial filter：Zymotic fluid removes thalline, by medical stone, quartz sand and expanded perlite hybrid filtering layer.Medical stone granule
Particle diameter be 1-2mm, the particle diameter of quartz sand is 0.5-0.8mm, and the particle diameter of expanded perlite is 1.5-2.5mm, its volume ratio
For medical stone：Quartz sand：Expanded perlite=3-5:2—3:5—8.Filtering 200-the 300mm of thickness constituted, filter layer
It is located in filtering tank, filtration temperature is in 70-80 DEG C, pressure 0.8-1MPa.
2. decolourize：Gained filtrate adjusts pH value to 5.5-6.5, and about 80 DEG C of temperature adds proper amount of active carbon into zymotic fluid,
It is incubated decolouring 20-30 minutes.
3rd, filter：Material is through plate-frame filtering.
4th, crystallize：Material adds alcohol to be crystallized into crystallizing tank stream.
5th, separate：Above-mentioned crystal solution is centrifuged, wet product is obtained.
6th, dissolve：Crystallization wet product is dissolved in water.
7th, ultrafiltration：The hydrating solution for crystallizing wet product is adjusted into pH value to 5.5-6.5, film desalination is crossed into ultrafiltration circulating tank.
8th, recrystallize：Lysate is added into crystallizing tank, stream plus alcohol, material is crystallized again.
9th, separate：Crystal solution is centrifuged, and obtains wet product.
10th, dry：Wet product is carried out to dry to obtain fine work.
Here three kinds of filtrates are placed in filtering tank, the screen cloth below provided with aperture 0.2, below again by aperture 50mm
Wire netting support.Expanded perlite granule can be cleared up by periodically recoiling, and can also regularly replace filtrate, used filtrate
Repeat and utilize after purified treatment.
Experiment is proved, before using activated carbon decolorizing, is first passed through the initial filter of above-mentioned filtrate, can both be filtered out a large amount of impurity,
Adsorbable a large amount of pigments, can significantly improve the purity of product again.The consumption of activated carbon can be greatly decreased, production cost is reduced.It is general
Shandong orchid purity of polysaccharide brings up to 99.5% by 95%, and whiteness brings up to 97.6% by 85%, and light transmittance is brought up to by 80%
98.8%.
Although the present invention is disclosed as above with embodiment, it is not limited to protection scope of the present invention, any ripe
Those skilled in the art is known, in the change and retouching done without departing from the spirit and scope of the invention, this hair all should be belonged to
The protection domain of bright appended claims.
Claims (7)
1. pulullan polysaccharide purifying technique, its feature comprises the following steps：Aureobasidium pullulans in fermentation medium with fermenting, fermentation
The zymotic fluid for removing thalline afterwards enters plate-frame filtering after decolouring, enters back into crystallizer, and stream plus alcohol are crystallized, and will be crystallized
Liquid squeezes into seperator and carries out isolated wet product, and the zymotic fluid of the removing thalline first passes through medical stone before being decolourized
Filter layer or quartz sand filtration layer or expanded perlite filter layer or medical stone, quartz sand and expanded perlite mixing were constituted
Filtering layer；The fermentation medium is made up of following composition and its components by weight percent：Sucrose 6-16%；Yeast extract 0.1-O.5%；Table catechu
Phenol 0.01-0.1%, NaCl 0.1-1%；Ergosterol 0.01-0.1%, MgSO4-7H2O 0.01-0.1%；Butanedioic acid 0.1-1%,
K2HPO4·3H2O 0.01-0.1%；(NH4)2SO4 0.01-0.1% and surplus sterilized water.
2. pulullan polysaccharide purifying technique according to claim 1, it is characterised in that the wet product, is dissolved in water, regulation
PH value is crossed after film desalination, desalination into ultrafiltration circulating tank and adds to lysate into crystallizing tank, stream plus alcohol are again to 5.5-6.5
Crystallization, crystal solution is centrifuged, and obtains wet feed, and fine work is dried to obtain in progress.
3. pulullan polysaccharide purifying technique according to claim 1, it is characterised in that the particle diameter of the medical stone granule is
1-2mm, the filtering 200-300mm of thickness constituted, Medical stone filtering layer are located in filtering tank, and filtration temperature is 70-80
DEG C, pressure 0.5-0.8MPa.
4. pulullan polysaccharide purifying technique according to claim 1, it is characterised in that the particle diameter of the quartz sand is
0.5-0.8mm, the filtering 100-150mm of thickness constituted, filter layer is located in filtering tank, filtration temperature at 70-80 DEG C,
Pressure 0.8-1MPa.
5. pulullan polysaccharide purifying technique according to claim 1, it is characterised in that the particle diameter of the expanded perlite is
1.5-2.5mm, the filtering 200-300mm of thickness constituted, filter layer is located in filtering tank, filtration temperature at 70-80 DEG C,
Pressure 0.8-1MPa.
6. pulullan polysaccharide purifying technique according to claim 1, it is characterised in that the particle diameter of the medical stone granule is
1-2mm, the particle diameter of quartz sand is 0.5-0.8mm, and the particle diameter of expanded perlite is 1.5-2.5mm.
7. the pulullan polysaccharide purifying technique according to claim 1-6, it is characterised in that medical stone：Quartz sand：Expansion is precious
Pearl rock volume ratio is 3-5:2—3:5-8, the filtering 200-300mm of thickness constituted, filter layer is located in filtering tank, filtering
Temperature is in 70-80 DEG C, pressure 0.8-1MPa.
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| CN201611267725.2A CN107056961A (en) | 2016-12-31 | 2016-12-31 | Pulullan polysaccharide purifying technique |
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| CN201611267725.2A CN107056961A (en) | 2016-12-31 | 2016-12-31 | Pulullan polysaccharide purifying technique |
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ID=59623764
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110407890A (en) * | 2019-07-18 | 2019-11-05 | 佛山市欧若拉生物科技有限公司 | The preparation method of sapindoside |
| CN113735991A (en) * | 2021-10-11 | 2021-12-03 | 天津科技大学 | Process for extracting pullulan from high-viscosity fermentation liquor |
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| CN102492740A (en) * | 2011-12-16 | 2012-06-13 | 天津北洋百川生物技术有限公司 | Method for producing poly(malic acid) and pullulan together by using Aureobasidium pullulans |
| CN103243135A (en) * | 2012-12-31 | 2013-08-14 | 天津北洋百川生物技术有限公司 | Novel culture medium for producing pulullan and method for fermenting and producing pulullan |
| CN103409480A (en) * | 2013-08-22 | 2013-11-27 | 天津北洋百川生物技术有限公司 | Method for producing Pulullans with different molecular weights |
| CN104448019A (en) * | 2014-12-05 | 2015-03-25 | 通辽梅花生物科技有限公司 | High-purity Pulullan extraction process |
| CN104479038A (en) * | 2014-12-05 | 2015-04-01 | 通辽梅花生物科技有限公司 | Pulullan purification technique |
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2016
- 2016-12-31 CN CN201611267725.2A patent/CN107056961A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102492740A (en) * | 2011-12-16 | 2012-06-13 | 天津北洋百川生物技术有限公司 | Method for producing poly(malic acid) and pullulan together by using Aureobasidium pullulans |
| CN103243135A (en) * | 2012-12-31 | 2013-08-14 | 天津北洋百川生物技术有限公司 | Novel culture medium for producing pulullan and method for fermenting and producing pulullan |
| CN103409480A (en) * | 2013-08-22 | 2013-11-27 | 天津北洋百川生物技术有限公司 | Method for producing Pulullans with different molecular weights |
| CN104448019A (en) * | 2014-12-05 | 2015-03-25 | 通辽梅花生物科技有限公司 | High-purity Pulullan extraction process |
| CN104479038A (en) * | 2014-12-05 | 2015-04-01 | 通辽梅花生物科技有限公司 | Pulullan purification technique |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110407890A (en) * | 2019-07-18 | 2019-11-05 | 佛山市欧若拉生物科技有限公司 | The preparation method of sapindoside |
| CN113735991A (en) * | 2021-10-11 | 2021-12-03 | 天津科技大学 | Process for extracting pullulan from high-viscosity fermentation liquor |
| CN113735991B (en) * | 2021-10-11 | 2022-06-03 | 天津科技大学 | Process for extracting pullulan from high-viscosity fermentation liquor |
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Application publication date: 20170818 |