CN107056344A - Containing Pleurotus citrinopileatus compost of honeysuckle plant refuse extracted after active material and preparation method thereof - Google Patents
Containing Pleurotus citrinopileatus compost of honeysuckle plant refuse extracted after active material and preparation method thereof Download PDFInfo
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- compost
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- 239000002361 compost Substances 0.000 title claims abstract description 64
- 241000392443 Pleurotus citrinopileatus Species 0.000 title claims abstract description 61
- 239000011149 active material Substances 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 241000205585 Aquilegia canadensis Species 0.000 title claims abstract 8
- 241000894006 Bacteria Species 0.000 claims abstract description 33
- 239000002994 raw material Substances 0.000 claims abstract description 18
- 239000000284 extract Substances 0.000 claims abstract description 13
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- 229920000742 Cotton Polymers 0.000 claims abstract description 12
- 239000000463 material Substances 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 17
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- 238000011081 inoculation Methods 0.000 claims description 8
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- 238000003756 stirring Methods 0.000 claims description 6
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- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 208000008454 Hyperhidrosis Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- 244000167230 Lonicera japonica Species 0.000 description 1
- 235000017617 Lonicera japonica Nutrition 0.000 description 1
- 244000103635 Lyophyllum ulmarium Species 0.000 description 1
- 235000015934 Lyophyllum ulmarium Nutrition 0.000 description 1
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 description 1
- 240000001462 Pleurotus ostreatus Species 0.000 description 1
- 235000001603 Pleurotus ostreatus Nutrition 0.000 description 1
- 208000027954 Poultry disease Diseases 0.000 description 1
- 240000000828 Tecoma stans Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
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- TWOWSRSKGJSZHZ-VRHDFFKNSA-N bouvardin Chemical compound C1=CC(OC)=CC=C1C[C@H](N(C)C(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N(C1=O)C)C2)C(=O)N[C@@H](C)C(=O)N(C)[C@H]1[C@@H](O)C(C=C1)=CC=C1OC1=CC2=CC=C1O TWOWSRSKGJSZHZ-VRHDFFKNSA-N 0.000 description 1
- BNOCDYMCAVSRBT-UHFFFAOYSA-N bouvardin Natural products COc1ccc(CC2N(C)C(=O)C(C)NC(=O)C(C)NC(=O)C3Cc4ccc(O)c(Oc5cccc(c5)C(O)C(N(C)C(=O)C(C)NC2=O)C(=O)N3C)c4)cc1 BNOCDYMCAVSRBT-UHFFFAOYSA-N 0.000 description 1
- 108010047824 bouvardin Proteins 0.000 description 1
- CJOBVZJTOIVNNF-UHFFFAOYSA-N cadmium sulfide Chemical compound [Cd]=S CJOBVZJTOIVNNF-UHFFFAOYSA-N 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical group O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 1
- 229940074393 chlorogenic acid Drugs 0.000 description 1
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 description 1
- 235000001368 chlorogenic acid Nutrition 0.000 description 1
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
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- 235000005822 corn Nutrition 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 208000013219 diaphoresis Diseases 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 235000021245 dietary protein Nutrition 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
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- 238000010791 quenching Methods 0.000 description 1
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- 238000012216 screening Methods 0.000 description 1
- 230000014860 sensory perception of taste Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
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- 235000015961 tonic Nutrition 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 229960000716 tonics Drugs 0.000 description 1
- 229930182493 triterpene saponin Natural products 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Landscapes
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Fertilizers (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a kind of containing the Pleurotus citrinopileatus compost for extracting the honeysuckle plant refuse after active material, it is made up of the raw material of following mass percent:Extract honeysuckle plant refuse 40 60%, corncob 20 50%, cotton seed hulls 0 20%, wheat bran 10% after active material.The invention also discloses the preparation method of the Pleurotus citrinopileatus compost.The present invention is used as the raw material for preparing Pleurotus citrinopileatus compost to extract the honeysuckle plant refuse after active material, honeysuckle plant refuse is effectively utilized, Pleurotus citrinopileatus cultivation cost is reduced, reduces energy resource consumption, adds bacterium bag success rate and plant mushroom benefit, China's mushroom class market is enriched.
Description
Technical field
It is more particularly to a kind of to be given up containing the honeysuckle plant extracted after active material the present invention relates to mushroom class technical field of cultivation
Pleurotus citrinopileatus compost of gurry and preparation method thereof.
Background technology
Pleurotus citrinopileatus also known as gold oyster mushroom, elm mushroom, gold mushroom, corn mushroom etc., are the successfully collection foods of exploitation cultivation in recent years
It is used for the Rare edible fungus new varieties of one with, medicine.Pleurotus citrinopileatus fructification shape such as trumpet flower, flower cluster gathers, mushroom color cadmium yellow, form
Elegant;Its is nutritious, main component be polysaccharide, dietary fiber and protein, rich in free amino acid, glutamic acid,
The taste components such as alanine, the edible sense of taste is very good, is mushroom class top grade;Pleurotus citrinopileatus have functions that strengthening by means of tonics, with reducing blood lipid, put down
Breathe heavily, it is antifatigue, improve the medical active such as immunity of organisms, kidney deficiency, impotence and dysentery etc. can be treated.The culturing raw material of Pleurotus citrinopileatus is very
It is many, such as cotton seed hulls, wood chip, corncob, but with increasingly deficient, planting industry industry restructuring and the cottonseed of forest tree resource
The comprehensive utilization of shell, corncob, causes culturing raw material price to increase substantially, and cultivates anxious in cost, production for reduction Pleurotus citrinopileatus
New culturing raw material need to be developed.
Caprifoliaceae woodbine honeysuckle (Lonicera japonica) is half evergreen winding rattan wood specific to China,
It is born in hill or artificial cultivation, perennial liana, mainly containing volatile oil, flavonoids, organic acid, triterpene saponin, inorganic elements
Etc. chemical composition.The traditional Chinese medical science is early, and it has proven convenient that its stem, leaf, spending and can be used as medicine, its sweet and slightly bitter taste is cold in nature, nontoxic, have promote the production of body fluid, quench the thirst,
The effects such as heat-clearing, scattered wind, inducing diaphoresis, be rare relieving summer-heat good medicine of passing the summer in a leisurely way.China's most area has honeysuckle planting,
To produce honeysuckle;Honeysuckle Shandong yield is maximum, and the quality of Henan production is good.Honeysuckle plant adaptability is strong, and life is vigorous, resistance to
It is cold drought-enduring, it is widely used, liked in recent years by people very much.Honeysuckle plant has significant antibacterial and anti-inflammation functions, in preventing and treating livestock and poultry
Disease areas tool has been widely used.It is referred to as " bouvardin ", the feelings being had serious consequences to abuse chemobiotic
Condition has certain mitigation.At present, the research and development and application of honeysuckle plant extracts are also increasingly extensive.
It is chlorogenic acid, the Huang for extracting honeysuckle plant to extract the honeysuckle plant refuse (rattan, leaf, flower etc.) after active material
Solid waste after the compositions such as ketone, polysaccharide.Through the detection of Jinan fruit research institute of Chinese national cooperation supply and marketing head office, activity is extracted
Contain protein 14.7%, crude fibre 11.1%, carbohydrate 61.7%, nitrogen in honeysuckle plant refuse after material
2.34%th, potassium 6.05%;Its pH value 4.32-5.21;Arsenic 0.19mg/kg (GB/T 5009.11-2003), lead 1.20mg/kg (GB
5009.12-2010), mercury 0.16mg/kg (GB/T 5009.17-2003), cadmium 0.29mg/kg (GB 5009.15-2014), chromium
0.062mg/kg(GB 5009.123-2014).Extract nutriment in the honeysuckle plant refuse after active material higher (outstanding
Its protein content is very high), harmful substance it is extremely low (even below food standard).It is long-term to stack, environmental pollution is easily caused, therefore
In the urgent need to effective way is converted to it.From the point of view of nutritional ingredient, the honeysuckle plant refuse extracted after active material can
For carrying out edible fungus culturing, but there is problems with:One is that particle size differs;Two be that contained nitrogen source is enriched, but is lacked
Effective carbon source;Three be that pH value is relatively low, the problems such as compost easily becomes sour.
Therefore, the honeysuckle plant refuse after active material will be extracted and still suffers from technological difficulties for the cultivation of edible mushroom,
It yet there are no the report of correlation.
The content of the invention
For above-mentioned the deficiencies in the prior art, it is an object of the invention to provide a kind of containing the honeysuckle extracted after active material
Pleurotus citrinopileatus compost of plant refuse and preparation method thereof.
The present invention is to extract the honeysuckle plant refuse after active material as the raw material for preparing Pleurotus citrinopileatus compost, effectively
Using honeysuckle plant refuse, reduce Pleurotus citrinopileatus cultivation cost, reduce energy resource consumption, add bacterium bag success rate and plant mushroom
Benefit, enriches China's mushroom class market.
To achieve the above object, the present invention is adopted the following technical scheme that:
The first aspect of the present invention is there is provided a kind of containing the Pleurotus citrinopileatus culture for extracting the honeysuckle plant refuse after active material
Material, is made up of the raw material of following mass percent:
Extract honeysuckle plant refuse 40-60%, corncob 20-50%, cotton seed hulls 0-20%, wheat bran after active material
10%.
It is preferred that, the Pleurotus citrinopileatus compost is made up of the raw material of following mass percent:
Extract honeysuckle plant refuse 60%, corncob 30%, wheat bran 10% after active material.
The second aspect of the present invention is there is provided the preparation method of above-mentioned Pleurotus citrinopileatus compost, and step is as follows:
(1) each raw material is mixed by mass percentage, add water stirring, obtains compound, adjust the pH to 8.0- of compound
8.5;
(2) heap fermentation is built, pack sterilizing produces Pleurotus citrinopileatus compost.
It is preferred that, in step (1), add water stirring when, material water mass ratio be 1:(1.1-1.2).
It is preferred that, in step (1), pH to 8.0-8.5 is adjusted with limewash.
It is preferred that, in step (2), the method for building heap fermentation is:Compound is built into heap, it is straight to beat several at the top of stockpile
The passage of logical stockpile bottom, 48h is kept when more than 60 DEG C of heap temperature.
It is preferred that, compound is built up to wide 1.5m, high 1.5m elongated heap.
It is preferred that, make a call to a passage every 0.5m at the top of stockpile.
It is preferred that, a diameter of 3-7cm of the passage;More preferably 5cm.
It is preferred that, in step (2), the method for pack sterilizing is:The compound built after heap fermentation is loaded into 18cm × 36-
In 38cm polyethylene plastic bag, 8-10h is kept to be sterilized at 100 DEG C.
The application of Pleurotus citrinopileatus compost prepared by the above method in Pleurotus citrinopileatus cultivation is also protection scope of the present invention.
The third aspect of the present invention is there is provided a kind of cultural method of Pleurotus citrinopileatus, and step is as follows:
(1) Pleurotus citrinopileatus compost is prepared as stated above;
(2) inoculation of Pleurotus citrinopileatus compost, bacterium germination;
(3) fruiting.
In step (2), during inoculation, the temperature of bacterium bag is 25-30 DEG C;Preferably 28 DEG C.
In step (2), the temperature of bacterium germination is 20-25 DEG C;Preferably 22 DEG C.
In step (3), the method for fruiting is:The fruiting mouthful of each stroke long 2-4cm (preferably 3cm) at two of bacterium bag, mouth is deep
0.3-0.7cm (preferably 0.5cm), 16-26 DEG C of temperature of control, air humidity 85-90%, illumination 150-1000lux, CO2<
0.1%.
Beneficial effects of the present invention:
(1) Pleurotus citrinopileatus compost component of the invention collocation is reasonable, the suitable Pleurotus citrinopileatus cultivation of the C/N ratios of compost, pH value;
Using the Pleurotus citrinopileatus of the compost cultivation of the present invention, its hyphal development is fast, and growth is vigorous, and mycelia is pure white dense, will not go out substantially
Situations such as existing bacterium bag pollutes, while first three damp biological transformation ratio is improved, mushroom tide number of days shortens (production cycle shortening), saves
Manpower and materials.
(2) Pleurotus citrinopileatus compost of the invention considerably reduces the consumption of cotton seed hulls and corncob, and can be lived to extracting
Property material after honeysuckle plant refuse effectively utilized, reduce environmental pollution, reduce production cost, be worthy to be popularized.
(3) in Pleurotus citrinopileatus compost preparation process of the invention, take 60 DEG C of compost (can with pack after top fermentation 48h
Make miscellaneous bacteria sprouting, the egg hatch adhered in culture medium), then high-temperature sterilization is carried out, energy when on the one hand can reduce sterilizing disappears
Consumption, on the other hand can thoroughly kill germ worm's ovum, can improve bacterium bag success rate.
(4) the Pleurotus citrinopileatus yield height of the compost production of the application present invention, quality are excellent.Add and extract bearing after active material
The compost of winter plant extract discarded object, can improve the protein content of Pleurotus citrinopileatus product, with the Pleurotus citrinopileatus compost of the present invention
First three damp biological transformation ratio be 47.69-57.20%, protein 30.2-38.5%;And using cotton seed hulls, corncob to be main raw
It is 34.72%, protein 24.8% to produce first three damp biological transformation ratio of raw material.
(5) using the production Pleurotus citrinopileatus of the honeysuckle plant extract discarded object after active material are extracted, than with cotton seed hulls, corncob
The mycelium germination for cultivating Pleurotus citrinopileatus is fast, often puts into 1 ton of compost, and net income can improve 1867.6-2788.4 members;Add to extract and live
Property material after the Pleurotus citrinopileatus bacterium bag of honeysuckle plant extract discarded object can all shorten the change of tide time, shorten the production cycle, ahead of time on
City, while saving labor cost, increases economic efficiency.
Embodiment
It is noted that described further below is all exemplary, it is intended to provide further instruction to the application.Unless another
Indicate, all technologies used herein and scientific terminology are with usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singulative
It is also intended to include plural form, additionally, it should be understood that, when in this manual using term "comprising" and/or " bag
Include " when, it indicates existing characteristics, step, operation, device, component and/or combinations thereof.
As background technology is introduced, lack in the prior art for extracting the honeysuckle plant refuse after active material
Effective Land use systems, based on this, present applicant proposes a kind of yellow containing the elm for extracting the honeysuckle plant refuse after active material
Mushroom compost and preparation method thereof.
There is provided the honeysuckle plant refuse after a kind of active material containing extraction in a kind of embodiment of the application
Pleurotus citrinopileatus compost, is made up of the raw material of following mass percent:
Extract honeysuckle plant refuse 40-60%, corncob 20-50%, cotton seed hulls 0-20%, wheat bran after active material
10%.
Because the particle size for extracting the honeysuckle plant refuse after active material differs, contained nitrogen source is enriched, but lacks
Effective carbon source, the present invention to the component of Pleurotus citrinopileatus compost reasonably optimize and revise, grain structure, the C/N of compost
Pleurotus citrinopileatus cultivation more suitable than, pH value, while first three damp mushroom biological transformation ratio is improved, mushroom tide number of days shortens that (production cycle contracts
It is short), use manpower and material resources sparingly.
There is provided the preparation method of above-mentioned Pleurotus citrinopileatus compost in the another embodiment of the application, step is as follows:
(1) each raw material is mixed by mass percentage, add water stirring, the mass ratio of material water is 1:(1.1-1.2), must be mixed
Material, the pH to 8.0-8.5 of compound is adjusted with limewash;
(2) compound is built up to wide 1.5m, high 1.5m elongated heap, several straight-through stockpile bottoms are beaten at the top of stockpile
Passage, keep 48h when more than 60 DEG C of heap temperature, be then charged into polyethylene plastic bag, 8- is kept at 100 DEG C after pack
10h is sterilized, and produces Pleurotus citrinopileatus compost.
The water content of Pleurotus citrinopileatus compost is an important indicator, if the water content of compost is too high, aeration is poor, bacterium
Silk is slow-growing, easily causes miscellaneous bacteria infection, or even fermentative acidification;If water content is too low, the later stage yields poorly.The present invention is yellow to elm
Screening is optimized in the water content of mushroom compost, as a result finds, the water content in compost of the present invention can make Pleurotus citrinopileatus bacterium
Silk growth is rapid, while avoiding the infection of miscellaneous bacteria mycelia.
The acid-base value of edible mushroom local environment, directly affects the activity of enzyme in hyphal cell, cell leakage and to gold
Belong to the absorbability of ion, the metabolism of edible mushroom is carried out in the presence of a series of enzymes, and enzyme is with catalytic effect
Protein, the effect of each enzyme has a corresponding pH value, it is too high it is too low all the vigor for making enzyme is reduced, so as to cause new old
Metabolism slows down or even stopped;The change of pH value, can also change the permeability of cell membrane, absorption of the influence cell to Cucumber, pH
Value is too high also to influence the inside and outside transmission of material and eupnea during bacterial metabolism.The pH value of the present invention is to coordinate the present invention
The most suitable pH value of compost.
Heap fermentation method is built using the present invention, the heap size of compost is suitable, and aeration is good, can make to build and be trained after heap
Nutriment fast speed reaches 60 DEG C, and normal-pressure sterilization can be rapidly achieved 100 DEG C after pack, is prevented effectively from the acidifying problem of compost, and
And save the energy.
There is provided a kind of cultural method of Pleurotus citrinopileatus in the yet another embodiment of the application, step is as follows:
(1) inoculation of Pleurotus citrinopileatus compost, bacterium germination;During inoculation, the temperature of bacterium bag is 25-30 DEG C, and the temperature of bacterium germination is 20-25
℃;
(2) fruiting:The long 2-4cm fruiting mouthful each stroke of two of bacterium bag, mouth depth 0.3-0.7,16-26 DEG C of temperature of control,
Relative air humidity 85-90%, illumination 150-1000lux, CO2< 0.1%.
Influence of the temperature to mycelial growth, on the one hand with the rise of temperature, hyphal cell accretion rate is accelerated, and promotes bacterium
The growth of silk and propagation;On the other hand, because cell main active substances protein, nucleic acid etc. are to sensitive, temperature it is too high its
Structure and activity can be destroyed and influenceed, and the temperature is the most thermophilic that Pleurotus citrinopileatus mycelia grows in the culture medium of the present invention
Degree.
Size, the depth of fruiting mouthful are relevant with time of buddingging, mushroom type, yield.Using the big of the fruiting mouthful designed by invention
Small and depth easily goes out the high-quality mushroom of lid stalklet length, and bacterium bag moisture is not easy to lose, production later stage yield is high.
In order that the technical scheme of the application can clearly be understood by obtaining those skilled in the art, below with reference to tool
The embodiment and comparative example of body describe the technical scheme of the application in detail.
Test material used is the conventional test material in this area in the embodiment of the present invention and comparative example, can be passed through
Commercial channel is commercially available.
The honeysuckle plant refuse extracted after active material used in the embodiment of the present invention, its containing protein 14.7%,
Crude fibre 11.1%, carbohydrate 61.7%, nitrogen 2.34%, potassium 6.05%;Arsenic 0.19mg/kg, lead 1.20mg/kg, mercury
0.16mg/kg, cadmium 0.29mg/kg, chromium 0.062mg/kg;Its pH value 4.32-5.21.
Embodiment 1:
The Pleurotus citrinopileatus compost of honeysuckle plant refuse after a kind of active material containing extraction, by following material according to quality
It is made after percentage mixing:Extract honeysuckle plant refuse 60%, corncob 30%, wheat bran 10% after active material.
Cultural method is as follows:
(1) first each raw material is well mixed, then adding water stirs, and be uniformly adjusted to compost pH value with limewash
8.5.Material-water ratio is controlled 1:1.2.
(2) it is every at the top of the elongated heap that the Compost in step (1) is not limited into wide 1.5m, high 1.5m, length, stockpile
The passage at a straight-through material bottom is made a call to thick 5cm wooden stick every 0.5m, 60 DEG C of 48h maintained above of heap temperature are treated, be then charged into 18cm ×
36cm polyethylene plastic bag.
(3) bacterium bag of step (2) is sterilized using normal-pressure sterilization mode, 8-10h is kept at 100 DEG C.
(4) when the bacterium bag cleaning of step (3) is cooled to 28 DEG C, two aseptic inoculation is carried out to bacterium bag.
(5) bacterium bag of step (4) is moved in draughty bacterium germination room and expects that temperature control exists in progress lucifuge culture, bag
22-24℃。
(6) 3cm fruiting mouthful is about at each stroke of bacterium bag two for covering with mycelia with blade, mouth depth 0.5cm controls temperature
16-26 DEG C, air humidity 85-90%, illumination 150-1000lux, CO2< 0.1%.
Embodiment 2
Containing the Pleurotus citrinopileatus compost for extracting the honeysuckle plant refuse after active material, by following material according to quality percentage
Than being made after mixing:Honeysuckle plant refuse 40%, corncob 50%, wheat bran 10% after active material are extracted, remaining is with real
Apply example 1.
Embodiment 3
Containing the Pleurotus citrinopileatus compost for extracting the honeysuckle plant refuse after active material, by following material according to quality percentage
Than being made after mixing:Extract honeysuckle plant refuse 50%, corncob 20%, cotton seed hulls 20%, wheat bran after active material
10%, remaining be the same as Example 1.
Reference examples 1:
The composition of compost:
Cotton seed hulls 45%, corncob 45%, wheat bran 10%, remaining be the same as Example 1.
Reference examples 2:
The composition of compost:
Extract honeysuckle plant refuse 60%, cotton seed hulls 30%, wheat bran 10%, remaining be the same as Example 1 after active material.
Reference examples 3:
The composition of compost:
Extract honeysuckle plant refuse 90%, wheat bran 10%, remaining be the same as Example 1 after active material.
The Pleurotus citrinopileatus comparative result cultivated with the planting material of the present invention with traditional planting material is shown in Table 1.
Table 1:Pleurotus citrinopileatus Field test
Note:(1) compost net income computational methods per ton:Compost per ton gives birth to moushroom yield × 8000 yuan/ton-raw materials for production
Cost (discarded object, cotton seed hulls, corncob, wheat bran etc.).
(2) raw materials for production cost is without facility, artificial, the energy, mushroom material (polybag, medicine, rope) etc..
The result that the traditional cultivation material of other formulas is drawn is similar with the result of upper table.
It can be drawn by table 1:The compost combination cultural method of the present invention is with tradition without bearing after extraction active material
The compost of winter plant refuse compares (embodiment 1,2,3 is compared with reference examples 1), and Pleurotus citrinopileatus protein content substantially increases,
Pollution rate is reduced, and change of tide number of days is significantly reduced, cost reduction, and the net income of compost per ton substantially increases;The present invention's
Compost combination cultural method with have containing extract active material after honeysuckle plant refuse compost compared with (embodiment 1,2,
3 compared with reference examples 2,3), the net income of compost fruiting amount per ton and compost per ton substantially increases.Compost of the present invention
Pleurotus citrinopileatus mycelium growth vigor is good, and bacterium germination is fast, and yield is high, and cost is low, and economic benefit, ecological benefits significantly, are worthy to be popularized.
The preferred embodiment of the application is the foregoing is only, the application is not limited to, for the skill of this area
For art personnel, the application can have various modifications and variations.It is all within spirit herein and principle, made any repair
Change, equivalent substitution, improvement etc., should be included within the protection domain of the application.
Claims (10)
1. it is a kind of containing the Pleurotus citrinopileatus compost for extracting the honeysuckle plant refuse after active material, it is characterised in that by following matter
The raw material of amount percentage is made:
Extract honeysuckle plant refuse 40-60%, corncob 20-50%, cotton seed hulls 0-20%, wheat bran after active material
10%.
2. Pleurotus citrinopileatus compost as claimed in claim 1, it is characterised in that be made up of the raw material of following mass percent:
Extract honeysuckle plant refuse 60%, corncob 30%, wheat bran 10% after active material.
3. the preparation method of the Pleurotus citrinopileatus compost described in claim 1 or 2, it is characterised in that step is as follows:
(1) each raw material is mixed by mass percentage, add water stirring, obtains compound, adjust the pH to 8.0-8.5 of compound;
(2) heap fermentation is built, pack sterilizing produces Pleurotus citrinopileatus compost.
4. preparation method as claimed in claim 3, it is characterised in that in step (1), add water stirring when, expect the mass ratio of water
For 1:(1.1-1.2).
5. preparation method as claimed in claim 3, it is characterised in that in step (2), the method for building heap fermentation is:Will mixing
Material builds heap, and several passages for leading directly to stockpile bottoms are beaten at the top of stockpile, 48h is kept when more than 60 DEG C of heap temperature.
6. preparation method as claimed in claim 3, it is characterised in that in step (2), packing the method sterilized is:Heap will be built
Compound after fermentation is fitted into bag, keeps 8-10h to be sterilized at 100 DEG C.
7. application of the Pleurotus citrinopileatus compost in Pleurotus citrinopileatus cultivation described in claim 1 or 2.
8. a kind of cultural method of Pleurotus citrinopileatus, it is characterised in that step is as follows:
(1) method as described in claim 3 prepares Pleurotus citrinopileatus compost;
(2) inoculation of Pleurotus citrinopileatus compost, bacterium germination;
(3) fruiting.
9. cultural method as claimed in claim 8, it is characterised in that in step (2), the temperature of bacterium bag is 25-30 during inoculation
DEG C, the temperature of bacterium germination is 20-25 DEG C;
It is preferred that, the temperature of bacterium bag is 28 DEG C during inoculation;The temperature of bacterium germination is 22 DEG C.
10. cultural method as claimed in claim 8, it is characterised in that in step (3), the method for fruiting is:The two of bacterium bag
The fruiting mouthful of each stroke long 2-4cm (preferably 3cm), mouth depth 0.3-0.7 (preferably 0.5cm) controls 16-26 DEG C of temperature, air wet
Spend 85-90%, illumination 150-1000lux, CO2< 0.1%.
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