CN107019797B

CN107019797B - Composition for improving penetrating capacity of coccidian oocysts on cells and preparation method thereof

Info

Publication number: CN107019797B
Application number: CN201610065366.6A
Authority: CN
Inventors: 陈庆忠; 安同伟; 曹桂敏; 李迎梅
Original assignee: Tianjin Hlinte Biotechnology Co ltd
Current assignee: Tianjin Hlinte Biotechnology Co ltd
Priority date: 2016-01-30
Filing date: 2016-01-30
Publication date: 2020-07-03
Anticipated expiration: 2036-01-30
Also published as: CN107019797A

Abstract

The invention relates to a composition for improving the ability of coccidian oocyst to penetrate cells and a preparation method thereof, wherein the composition comprises the following components in parts by weight: 5-30 parts of houttuynia cordata extract; 5-30 parts of rhubarb extract; 0.1-0.5 part of guar gum hydroxypropyl trimethyl ammonium chloride; 5-30 parts of lysozyme; 10-90 parts of sterilized water; the invention has scientific and reasonable formula composition, simple process, convenient use and lower cost; the composition improves the quantity of the sporozoites in the oocysts entering the epithelial cells of the intestinal tract and rapidly fixes the value in the intestinal tract cells by utilizing the effects of destroying the cell wall structure, increasing the penetration force to the cell membrane, reducing the resistance of cell sap and the like.

Description

Composition for improving penetrating capacity of coccidian oocysts on cells and preparation method thereof

Technical Field

The invention belongs to the field of biological products for livestock, and particularly relates to a composition for improving the cell penetrating capability of coccidian oocysts and a preparation method thereof.

Background

At present, the domestic breeding trend is intensified and large-scale directional development, according to the national food safety regulation, some antibiotics and antiparasitic drugs are prohibited to be added into feed, drug residues are also strictly controlled, the antiparasitic diseases, particularly coccidiosis, are prevented and controlled by coccidian vaccines basically, according to the clinical effect, the immune effect of the common coccidian vaccines is not ideal, coccidian oocysts rupture oocyst walls under the mechanical pressure of muscular stomach and release sporozoites which need breathing movement, carbohydrates stored in vivo can be reduced in a short time, if the sporozoites can not enter cells rapidly, the activity can be lost, particularly in hot summer, the high-incidence season of the coccidia is the season, at the moment, the water drinking amount of chicken flocks is increased, and the content after the sporozoites are removed from the body can be discharged along with the discharge of the outside, so that the immunity is reduced or failed, therefore, according to the biological characteristics of coccidia, the research on a composition for improving the cell penetrating capability of coccidia oocysts is important.

Disclosure of Invention

The invention aims to provide a composition for improving the cell penetrating capability of coccidian oocysts and a preparation method thereof, compared with the composition only using a vaccine, the composition has the advantages of obvious immune effect, high OPG oocyst index in excrement, high feed conversion rate, quick weight increment and low mortality of chicken flocks.

Another object of the present invention is to provide a composition for improving the ability of coccidian oocysts to penetrate cells and a method for preparing the same.

The invention is realized by the following technical scheme:

a composition for improving the ability of coccidian oocyst to penetrate cells and a preparation method thereof are disclosed, wherein the protective solution comprises the following components in parts by weight:

moreover, the components of the composition and the optimal weight part ratio thereof are respectively as follows:

a composition for improving the ability of coccidian oocysts to penetrate cells and a preparation method thereof are disclosed, wherein the preparation method comprises the following steps:

(1) sequentially weighing the houttuynia cordata extract, the rheum officinale extract, the guar gum hydroxypropyl trimethyl ammonium chloride and the lysozyme according to the weight ratio, putting the houttuynia cordata extract, the rheum officinale extract, the guar gum hydroxypropyl trimethyl ammonium chloride and the lysozyme into a liquid preparation tank, then adding sterile water until the houttuynia cordata extract, the rheum officinale extract, the guar gum hydroxypropyl trimethyl ammonium chloride and the lysozyme are submerged in the materials, soaking the materials at room temperature for 1 hour, then continuously adding the rest sterile water, stirring the materials uniformly until the materials are completely dissolved, filtering the materials by;

(2) the liquid preparation tank is heated to the pressure of 0.05Mpa, the evaporator discharges condensed water once in 15 minutes, and the condensed steam of the heater is discharged once every two hours. Naturally cooling indoors for later use to obtain solution B for later use;

(3) filtering the solution B obtained in the step (2) by using a 0.22um polyethersulfone filter element, adjusting the pH value of the solution to 6.0-7.0, stirring for 15 minutes, filtering by using a 0.22um polyethersulfone filter element again to obtain a solution C, and directly feeding the solution C into a filling machine through a pipeline;

(4) and (4) filling the solution C obtained in the step (3) into a dried medical bottle to obtain the composition for improving the cell penetrating capability of coccidian oocysts.

The invention has the advantages and beneficial effects that:

1. the invention aims to provide a composition for improving the cell penetrating capability of coccidian oocysts and a preparation method thereof, and the composition has scientific and reasonable formula composition, simple process, convenient use and lower cost;

2. the composition improves the quantity of the sporozoites in the oocysts entering the epithelial cells of the intestinal tract and rapidly fixes the value in the intestinal tract cells by utilizing the effects of destroying the cell wall structure, increasing the penetration force to the cell membrane, reducing the resistance of cell sap and the like;

3. the invention can be used for separating and purifying samples with high-intensity infection, and can be further used for samples with weak infection.

Detailed Description

The invention is further illustrated by the following examples. It should be noted that: the following examples are illustrative and not intended to be limiting, and are not intended to limit the scope of the invention.

The houttuynia cordata extract, the rheum officinale extract, the guar gum hydroxypropyl trimethyl ammonium chloride and the lysozyme in the components can be purchased in the market.

Example 1

The composition for improving the cell penetrating capacity of coccidian oocysts comprises the following components in parts by weight: 5kg of houttuynia cordata extract, 5kg of rheum officinale extract, 0.1kg of guar gum hydroxypropyl trimethyl ammonium chloride, 5kg of lysozyme and 10kg of sterilized water.

Example 2

The composition for improving the cell penetrating capacity of coccidian oocysts comprises the following components in parts by weight: 30kg of houttuynia cordata extract, 30kg of rheum officinale extract, 0.5kg of guar gum hydroxypropyl trimethyl ammonium chloride, 30kg of lysozyme and 90kg of sterilized water.

The procedure for the preparation is the same as in example 1.

Example 3

The composition for improving the cell penetrating capacity of coccidian oocysts comprises the following components in parts by weight: 15kg of houttuynia cordata extract, 15kg of rhubarb extract, 0.3kg of guar gum hydroxypropyl trimethyl ammonium chloride, 15kg of lysozyme and 65kg of sterilized water.

The procedure for the preparation is the same as in example 1.

Clinical application

Description of the test:

clinical comparative tests are carried out in 03 months in 2015 by Qinhuangdai Dai group breeding companies, 300 chickens of 7 days old are taken for tests, each group comprises 100 chickens, namely a blank group, a vaccine immunization group sold in the market and a group using the feed additive, and three groups are respectively fed by normal sterilized water and non-antibiotic feed; the immune vaccine uses the vaccine of the positive typical coccidium of Guangdong Foshan, 1000 parts of feather/bottle, 400 +/-10 parts of feather, and the chicken is given according to the instruction for use, and the composition of the invention is used by freely drinking 0.1ml per chicken, and can be used with the vaccine. And (3) performing virus attack on the two immunization groups at 8 days after immunization, wherein 1 ten thousand per group is injected with crop through mouth, and observing the situation of the chicken flocks at 8 days after virus attack.

Table test comparison table

Group of	Blank space	Vaccine immunization group sold on market	Immunization group of the invention
				Condition of stool	Is normal	Bloody stool after toxic materials are attacked	Is normal
Status of chicken flock	Is normal	The individual chicken had a deep and depressed mind	Is normal
				Rate of protection	100 portions of	85.4 portions	99.2 parts of
Feed-meat ratio of whole raising period	2:1	2.4:1	2:1
				Average weight (g) in slaughter	5040	4800	5000
OPG in feces	0	5.6 ten thousand/g	0.3 ten thousand/g

And (4) conclusion: the indexes of the fecal condition, the chicken flock state, the protection rate, the feed-meat ratio of the whole feeding period, the slaughter average body weight, the OPG in the feces and the like of the immune group are close to the empty chicken, so that the immune protection rate of the composition is higher in coccidian, the amount and the speed of the sporulated oocysts in the vaccine entering cells after being excysted are higher, and the clinical effect of the composition is more obvious.

Claims

1. A drinkable composition for use with coccidial vaccines, comprising: the composition comprises the following components in parts by weight:

5-30 parts of houttuynia cordata extract;

5-30 parts of rhubarb extract;

0.1-0.5 part of guar gum hydroxypropyl trimethyl ammonium chloride;

5-30 parts of lysozyme;

10-90 parts of sterilized water.

2. A drinkable composition for use with coccidian vaccines as claimed in claim 1 wherein: the composition comprises the following components in parts by weight:

5 parts of houttuynia cordata extract;

5 parts of rhubarb extract;

0.1 part of guar gum hydroxypropyl trimethyl ammonium chloride;

10 parts of lysozyme;

80 parts of sterilized water.

3. A method of preparing a drinkable composition for use with coccidial vaccines as described in claim 1, comprising the steps of: the preparation method comprises the following steps:

(1) sequentially weighing the houttuynia cordata extract, the rheum officinale extract, the guar gum hydroxypropyl trimethyl ammonium chloride and the lysozyme according to the weight ratio, putting the houttuynia cordata extract, the rheum officinale extract, the guar gum hydroxypropyl trimethyl ammonium chloride and the lysozyme into a liquid preparation tank, adding sterile water until the houttuynia cordata extract, the rheum officinale extract, the guar gum hydroxypropyl trimethyl ammonium chloride and the lysozyme are submerged in the liquid preparation tank, soaking the mixture at room temperature for 3 hours, continuously adding the rest sterile water, stirring the mixture uniformly until the fed materials are completely dissolved, filtering the mixture by;

(2) heating the liquid preparation tank to a pressure of 0.05Mpa, discharging condensed water once by an evaporator for 15 minutes, discharging condensed steam of a heater once every two hours, and naturally cooling indoors for later use to obtain a solution B for later use;

(3) filtering the solution B obtained in the step (2) by using a 0.22 mu m polyether sulfone filter element, adjusting the pH value of the solution to 6.0-7.0, stirring for 15 minutes, filtering by using the 0.22 mu m polyether sulfone filter element again to obtain a solution C, and directly feeding the solution C into a filling machine through a pipeline;