CN106983778A - A kind of Moringa hypoglycemic activity composition and its application - Google Patents
A kind of Moringa hypoglycemic activity composition and its application Download PDFInfo
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- CN106983778A CN106983778A CN201710344870.4A CN201710344870A CN106983778A CN 106983778 A CN106983778 A CN 106983778A CN 201710344870 A CN201710344870 A CN 201710344870A CN 106983778 A CN106983778 A CN 106983778A
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- moringa
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Abstract
The present invention relates to the component composition of a kind of Moringa hypoglycemic activity composition and its application, including following masses part：0~10 part of leaf of Moringa total saposins, 0~20 part of total flavones of Moringa oleifera leaf, 0~5 part of leaf of Moringa total alkaloid, 20~65 parts of auxiliary material, are zero when wherein leaf of Moringa total saposins, total flavones of Moringa oleifera leaf are different with leaf of Moringa total alkaloid.The present invention raw material both from Moringa in itself, pass through Phytochemistry extraction separation method, blood sugar reducing component therein is subjected to separating-purifying respectively, obtain leaf of Moringa total saposins, total flavones of Moringa oleifera leaf and leaf of Moringa total alkaloid, the Traditional Chinese medicine for reducing blood glucose of compatibility integration of drinking and medicinal herbs prepares Moringa blood sugar reducing food, health products etc. jointly again, and active constituent concentration is high, blood sugar decreasing effect is obvious, without any toxicity and side effects, it can be eaten for a long time, it is hypoglycemic while can also nutrition and health care.
Description
Technical field
The present invention relates to a kind of Moringa hypoglycemic activity composition and its application, category natural product extraction application technology neck
Domain.
Background technology
Moringa (Moringa oleifera) belong to Moringaceae (Moringaceae) Moringa (Moringa Adans.)
Torrid zone fallen leaves woody vegetables and oilseed plant, Moringa blade and Fruit pod are rich in several mineral materials, vitamin, protein and amino acid
Etc. active ingredient, and fat content is relatively low, its active component have anti-oxidant and protection liver, hypoglycemic, reducing blood lipid and blood pressure,
The health-care efficacies such as anti-inflammation.
Moringa complete stool edible, containing abundant protein, lipid, carbohydrate and vitamin etc., in many developing countries
It is used as the dietary supplements generally used.In the west African state, Moringa is otherwise known as miracle tree（miracle tree）, church of the world
Servers Organization is just directed to promoting the cultivation of Moringa and edible process technology, recommends everyone daily edible soupspoon of local resident peppery
Wooden leaf powder, to substitute the import medicine of costliness, improves resident's physical function, treatment malnutrition or the disease being induced by it.
UCLA nutritionist points out, daily a soupspoon（About 15g）Leaf of Moringa powder, you can meet a children tieed up needed for one day
The intake of raw element and essential amino acid.According to Liu Changfen etc. research, it is known that the leaf of Moringa nutritional ingredient of Different Provenances is
Difference, but rich in nutrition content, content are balanced.There is research to point out that Moringa leaf protein and total dietary fiber are higher simultaneously,
Fat content is low, and vitamin, the digestibility of Grapeseed Oil, absorptivity are all higher than other plant foods[50].Moringa
Seed contains abundant fat, protein and liposoluble vitamin, can be processed as oil ben and high protein feed best in quality.
Dong little Ying points out that the protein in Moringa benevolence is 4 times of milk, and benevolence contains 17 kinds of amino acid, and 8 kinds of human bodies must ammonia
Base acid content is also enriched very much.
Also containing feature organic molecules such as abundant saponins compound, flavones, organic sulfur compounds in the leaf of Moringa,
Though human body is smaller to the demand of these materials, plays raising Abwehrkraft des Koepers, remove the effect such as free radical and anticancer, mesh
It is preceding it is not yet found that to the report of Moringa these feature organic molecules further investigation.There is research to point out, also contain in leaf of Moringa
There are the saponin(e and aldehydes matter of some anti-nutrition components, but saponin(e is inert, and phenols dosage also far smaller than causes in animal
The degree of restriction of poison.Also alkaloid component is contained in Moringa benevolence, but these compositions only just produce cardiac stimulant in heavy dose intake
And the effect of rise blood pressure.Because these anti-nutrition component contents are few or it is unobvious to act on, hold again in general process
It is easily destroyed, therefore toxic side effect will not be produced to human body.
Diabetes (Diabetes Mellitus, DM) are a classes due to insulin secretion definitely or relative deficiency, glycometabolism
Endocrine metabolism disease caused by disorder by principal character of hyperglycaemia.Whole world diabetic's number drastically rises at present
Height, prevention and treatment diabetes and its complication are very urgent.With the further investigation to pathogenesis of diabetes mellitus, according to hair
Sick key target spot mechanism of action, which finds effective hypoglycemic medicine, turns into new focus.
Ndong etc., as animal model, glucose tolerance is carried out to rat using non-obese type diabetes B (GK) rat
Experiment, oral Gavage glucose dose is in terms of 29/kg, and leaf of Moringa pulvis amount is based on 200mg/kg, and detection mouse feeding is preceding and raises
2h change of blood sugar, as a result finds that the fasting blood glucose level and level of postprandial blood sugar that do not feed the GK rats of leaf of Moringa powder are high after feeding
In control rats, show that feeding leaf of Moringa powder can improve the glucose tolerance of diabetic mice.
Studied and found by the hypoglycemic animal experiment of total flavones of Moringa oleifera leaf (TFM), TFM can significantly reduce alloxan
The blood glucose of diabetic mice, while SOD in serum vigor can be improved, reduces Content of MDA, and TFM is to normal mouse
Blood sugar level without influence.
The Moringa root extract that Gupta etc. gives respectively to diabetes rat, as a result shows, moringa root can be reduced in serum
Glucose and content of nitric oxide, increase serum insulin and protein level；Pancreatic tissue antioxidant levels can be increased, reduced
The content of Thiobarbituric Acid Reactive Substance, with obvious hypoglycemic and antioxidation activity.
The content of the invention
In order to solve the deficiencies in the prior art, there is provided a kind of Moringa hypoglycemic activity by primary raw material of Moringa by the present invention
Composition and its application, then compatibility other Traditional Chinese medicine for reducing blood glucose powder prepare the hypoglycemic pressed candy of Moringa, oral effervescent tablet, piece
The formulations such as agent, capsule, and applied in functional food and diabetic's nutritional supplement arts.
The technical solution adopted by the present invention is as follows：A kind of Moringa hypoglycemic activity composition, includes the group of following masses part
It is grouped into：
0~10 part of leaf of Moringa total saposins, 0~20 part of total flavones of Moringa oleifera leaf, 0~5 part of leaf of Moringa total alkaloid, auxiliary material 20~65
Part, it is zero when wherein leaf of Moringa total saposins, total flavones of Moringa oleifera leaf are different with leaf of Moringa total alkaloid.
The auxiliary material is cornstarch, microcrystalline cellulose, D-sorbite, fruit powder, mannitol, maltodextrin, lactose, sugarcane
One or more in sugar, vitamin C, sodium acid carbonate, citric acid, calcium carbonate.
The Moringa hypoglycemic activity composition also includes kudzu-vine root powder and/or yam flour, and kudzu-vine root powder and yam flour are ultra micro
Powder.
The leaf of Moringa total saposins are made through following operation：By leaf of Moringa that is fresh or drying, pass through 20 mesh medicines after pulverizing
Sieve, addition 1 is calculated as by solid-to-liquid ratio g/mL:5~10, deionized water is added, is decocted 2~3 times, 2~3h every time, merging filtrate concentration
To the concentrated solution that relative density is 0.9~1.2mg/mL, 95% ethanol is added, repeatedly to add on a small quantity, does not stop stirring, directly
To flocky precipitate is obtained, stirring without precipitation untill continuing to separate out；12~24h is placed in 4~6 DEG C of refrigerators, suction filtration, filtrate subtracts
Pressure concentration, 40~50 DEG C of thickening temperature volatilizes and leaf of Moringa total saposins is obtained after solvent.
The total flavones of Moringa oleifera leaf is made through following operation：Dry leaf of Moringa is crushed, sieved by 20 mesh medicines, by liquid material
Volume ratio 5~10:1, add concentration and soaked for 40~60v/v% ethanol after 20~30min, refluxing extraction 2~3 times, every time 2
~3h, merging filtrate is concentrated under reduced pressure, 50~60 DEG C of thickening temperature, is concentrated into medicinal extract completely without ethanol flavor, obtains leaf of Moringa total
Extract；Leaf of Moringa total extract is passed through into polycaprolactam resin, with distillation 8~10 retention volumes of water elution, to wash-off
Liquid is clarified, and is changed 4~5 retention volumes of ethanol elution that concentration is 70 v/v %, flow velocity is 3mL/ming, is collected eluent,
Ethanol is reclaimed, total flavones of Moringa oleifera leaf is obtained.
The leaf of Moringa total alkaloid is made through following operation：Dry moringa root is crushed, sieved by 20 mesh medicines, by liquid
Expect volume ratio 5~10:1, it is that 1~5wt% aqueous sulfuric acids are decocted 2~3 times with concentration, each 2h, merging filtrate, then use concentration
PH is adjusted to neutrality for 1~5wt% calcium hydroxide, is then extracted, extracted 3~5 times repeatedly with dichloromethane, reclaims two
Chloromethanes, obtains leaf of Moringa total alkaloid.
The Moringa hypoglycemic activity composition is in the formulations such as pressed candy, oral effervescent tablet, lozenge, capsule are prepared
Application.
Advantage and effect that the present invention possesses：The raw material of the present invention in itself, is extracted both from Moringa by Phytochemistry
Separation method, separating-purifying is carried out by blood sugar reducing component therein respectively, obtains leaf of Moringa total saposins, total flavones of Moringa oleifera leaf and peppery
Wooden leaf total alkaloid, then Traditional Chinese medicine for reducing blood glucose Chinese yam, the root of kudzu vine etc. of compatibility integration of drinking and medicinal herbs prepare Moringa blood sugar reducing food, health care jointly
Product etc., active constituent concentration is high, blood sugar decreasing effect is obvious, in the integration of drinking and medicinal herbs catalogue that planning commission's announcement is all defended using country
Plant be raw material, without any toxicity and side effects, can be eaten for a long time, it is hypoglycemic and meanwhile can also nutrition and health care, wherein Chinese yam is hypoglycemic same
When also there is filling liver kidney, invigorating the spleen gas, root of kudzu vine relieving superficies by cooling Shengyang Zhixie can expand blood vessel, hypotensive is hypoglycemic.
Embodiment
Below by embodiment, the present invention will be further described.
Embodiment 1
Pressed candy is made up of the component of following masses part：
10 parts of total flavones of Moringa oleifera leaf, 5 parts of lactose, 60 parts of maltodextrin, 10 parts of kudzu-vine root powder, 10 parts of yam flour.
Said components are mixed, direct powder compression, prepare leaf of Moringa pressed candy.
The total flavones of Moringa oleifera leaf is made through following operation：Dry leaf of Moringa is crushed, sieved by 20 mesh medicines, by liquid material
Volume ratio 8:1, after adding concentration for 50v/v% ethanol immersion 25min, refluxing extraction 2 times, each 3h, merging filtrate, decompression
Concentration, 55 DEG C of thickening temperature is concentrated into medicinal extract completely without ethanol flavor, obtains leaf of Moringa total extract；Leaf of Moringa is always extracted
Thing passes through polycaprolactam resin, with distillation 9 retention volumes of water elution, and to eluate clarification, it is 70 v/v %'s to change concentration
4 retention volumes of ethanol elution, flow velocity is 3mL/ming, collects eluent, reclaims ethanol, obtains total flavones of Moringa oleifera leaf.
Embodiment 2
Oral effervescent tablet is made up of the component of following masses part：5 parts of leaf of Moringa total saposins, 1 part of vitamin C, sodium acid carbonate 20
Part, 20 parts of citric acid, 20 parts of calcium carbonate.
After said components are mixed, appropriate absolute ethyl alcohol is added using equal increments method, softwood processed all passes through 10 mesh
Medicine is sieved, and is prepared into wet granular, and 30~40min is dried at 50~60 DEG C, then is sieved by 10 mesh medicines, and whole grain is fragrant by proper amount of edible
Essence is mixed into absolute ethyl alcohol, is sprayed at particle surface, and tabletting is carried out at room temperature, produces leaf of Moringa oral effervescent tablet.
The leaf of Moringa total saposins are made through following operation：By leaf of Moringa that is fresh or drying, pass through 20 mesh medicines after pulverizing
Sieve, addition 1 is calculated as by solid-to-liquid ratio g/mL:8, deionized water is added, is decocted 2 times, each 3h, merging filtrate is concentrated into relative density
For 0.9~1.2mg/mL concentrated solution, 95% ethanol is added, repeatedly to add on a small quantity, does not stop stirring, until obtaining cotton-shaped
Sediment, stirring without precipitation untill continuing to separate out；20h, suction filtration, filtrate decompression concentration, thickening temperature 45 are placed in 5 DEG C of refrigerators
DEG C, volatilize and leaf of Moringa total saposins are obtained after solvent.
Embodiment 3
Capsule is made up of the component of following masses part：5 parts of total flavones of Moringa oleifera leaf, 5 parts of leaf of Moringa total alkaloid, beta-schardinger dextrin 1
Part, 10 parts of lactose, 20 parts of cornstarch.
After said components are mixed, appropriate absolute ethyl alcohol is added using equal increments method, softwood processed all passes through 10 mesh
Medicine is sieved, and is prepared into wet granular, and 30~40min is dried at 50~60 DEG C, then is sieved by 10 mesh medicines, and whole grain loads No. 0 hard shell capsules
In shell, leaf of Moringa capsule is produced.
The total flavones of Moringa oleifera leaf is made through following operation：Dry leaf of Moringa is crushed, sieved by 20 mesh medicines, by liquid material
Volume ratio 5:1, after adding concentration for 40v/v% ethanol immersion 20min, refluxing extraction 3 times, each 2h, merging filtrate, decompression
Concentration, 50 DEG C of thickening temperature is concentrated into medicinal extract completely without ethanol flavor, obtains leaf of Moringa total extract；Leaf of Moringa is always extracted
Thing passes through polycaprolactam resin, with distillation 8 retention volumes of water elution, and to eluate clarification, it is 70 v/v %'s to change concentration
4 retention volumes of ethanol elution, flow velocity is 3mL/ming, collects eluent, reclaims ethanol, obtains total flavones of Moringa oleifera leaf.
The leaf of Moringa total alkaloid is made through following operation：Dry moringa root is crushed, sieved by 20 mesh medicines, by liquid
Expect volume ratio 5:1, it is that 1wt% aqueous sulfuric acids are decocted 2 times with concentration, each 2h, merging filtrate, then the hydrogen for being 1wt% with concentration
Calcium oxide adjusts pH to neutrality, is then extracted with dichloromethane, extracts 3 times repeatedly, reclaims dichloromethane, obtains leaf of Moringa
Total alkaloid.
Embodiment 4
Lozenge is made up of the component of following masses part：
10 parts of leaf of Moringa total saposins, 20 parts of total flavones of Moringa oleifera leaf, 2 parts of leaf of Moringa total alkaloid, 5 parts of lactose, microcrystalline cellulose 5
Part, 5 parts of mannitol, 5 parts of yam flour.
Said components are mixed, direct powder compression, prepare leaf of Moringa lozenge.
The leaf of Moringa total saposins are made through following operation：By leaf of Moringa that is fresh or drying, pass through 20 mesh medicines after pulverizing
Sieve, addition 1 is calculated as by solid-to-liquid ratio g/mL:5~10, deionized water is added, is decocted 2~3 times, 2~3h every time, merging filtrate concentration
To the concentrated solution that relative density is 0.9~1.2mg/mL, 95% ethanol is added, repeatedly to add on a small quantity, does not stop stirring, directly
To flocky precipitate is obtained, stirring without precipitation untill continuing to separate out；12~24h is placed in 4~6 DEG C of refrigerators, suction filtration, filtrate subtracts
Pressure concentration, 40~50 DEG C of thickening temperature volatilizes and leaf of Moringa total saposins is obtained after solvent.
The total flavones of Moringa oleifera leaf is made through following operation：Dry leaf of Moringa is crushed, sieved by 20 mesh medicines, by liquid material
Volume ratio 10:1, after adding concentration for 60v/v% ethanol immersion 30min, refluxing extraction 3 times, each 2h, merging filtrate, decompression
Concentration, 60 DEG C of thickening temperature is concentrated into medicinal extract completely without ethanol flavor, obtains leaf of Moringa total extract；Leaf of Moringa is always extracted
Thing passes through polycaprolactam resin, with distillation 10 retention volumes of water elution, and to eluate clarification, it is 70 v/v %'s to change concentration
5 retention volumes of ethanol elution, flow velocity is 3mL/ming, collects eluent, reclaims ethanol, obtains total flavones of Moringa oleifera leaf.
The leaf of Moringa total alkaloid is made through following operation：Dry moringa root is crushed, sieved by 20 mesh medicines, by liquid
Expect volume ratio 5~10:1, it is that 1~5wt% aqueous sulfuric acids are decocted 2~3 times with concentration, each 2h, merging filtrate, then use concentration
PH is adjusted to neutrality for 1~5wt% calcium hydroxide, is then extracted, extracted 3~5 times repeatedly with dichloromethane, reclaims two
Chloromethanes, obtains leaf of Moringa total alkaloid.
The model of Moringa anti-diabetic zoopery uses chemicals revulsion, with alloxan inducing mild and moderate glycosuria
Sick rat model, rat carries out gastric infusion according to 300mg/kg dosage, as a result as shown in the table.
The Moringa anti-diabetic results of animal of table 1
Test result indicates that, using alloxan diabetes rats as animal model, using antidiabetic drug melbine as control, carry out peppery
The hypoglycemic activity research of wooden the different extracted parts and extract, as a result shows, total flavones of Moringa oleifera leaf, leaf of Moringa total saposins have
The effect of blood glucose is reduced, as a result successive administration shows after 7 weeks, Moringa difference extract can significantly reduce blood glucose in diabetic rats,
Serum MDA content is reduced simultaneously, while the vigor of serum superoxide dismutases can also be improved.
Claims (7)
1. a kind of Moringa hypoglycemic activity composition, it is characterised in that the component composition including following masses part：
0~10 part of leaf of Moringa total saposins, 0~20 part of total flavones of Moringa oleifera leaf, 0~5 part of leaf of Moringa total alkaloid, auxiliary material 20~65
Part, it is zero when wherein leaf of Moringa total saposins, total flavones of Moringa oleifera leaf are different with leaf of Moringa total alkaloid.
2. Moringa hypoglycemic activity composition according to claim 1, it is characterised in that：The auxiliary material be cornstarch,
Microcrystalline cellulose, D-sorbite, fruit powder, mannitol, maltodextrin, lactose, sucrose, vitamin C, sodium acid carbonate, citric acid, carbon
One or more in sour calcium.
3. Moringa hypoglycemic activity composition according to claim 1, it is characterised in that：The Moringa hypoglycemic activity group
Compound also includes kudzu-vine root powder and/or yam flour, and kudzu-vine root powder and yam flour are Ultramicro-powder.
4. Moringa hypoglycemic activity composition according to claim 1, it is characterised in that：Under the leaf of Moringa total saposins warp
Row operation is made：By leaf of Moringa that is fresh or drying, sieved after pulverizing by 20 mesh medicines, addition 1 is calculated as by solid-to-liquid ratio g/mL:5~
10, deionized water is added, is decocted 2~3 times, 2~3h every time, it is 0.9~1.2mg/mL's that merging filtrate, which is concentrated into relative density,
Concentrated solution, adds 95% ethanol, repeatedly to add on a small quantity, does not stop stirring, until obtaining flocky precipitate, stirring is to without heavy
Form sediment untill continuing to separate out；12~24h is placed in 4~6 DEG C of refrigerators, suction filtration, filtrate decompression concentration, 40~50 DEG C of thickening temperature is waved
Leaf of Moringa total saposins are obtained after dry solvent.
5. Moringa hypoglycemic activity composition according to claim 1, it is characterised in that：Under the total flavones of Moringa oleifera leaf warp
Row operation is made：Dry leaf of Moringa is crushed, sieved by 20 mesh medicines, by liquid material volume ratio 5~10:1, add concentration be 40~
60v/v% ethanol is soaked after 20~30min, refluxing extraction 2~3 times, and 2~3h, merging filtrate, are concentrated under reduced pressure every time, concentration temperature
50~60 DEG C of degree, is concentrated into medicinal extract completely without ethanol flavor, obtains leaf of Moringa total extract；By leaf of Moringa total extract by poly-
Acid amides polymeric adsorbent, with distillation 8~10 retention volumes of water elution, to eluate clarification, changes the ethanol that concentration is 70 v/v %
4~5 retention volumes are eluted, flow velocity is 3mL/ming, collect eluent, reclaimed ethanol, obtain total flavones of Moringa oleifera leaf.
6. Moringa hypoglycemic activity composition according to claim 1, it is characterised in that：The leaf of Moringa total alkaloid warp
Following operation is made：Dry moringa root is crushed, sieved by 20 mesh medicines, by liquid material volume ratio 5~10:1, with concentration be 1~
5wt% aqueous sulfuric acids decoct 2~3 times, each 2h, merging filtrate, then with concentration for 1~5wt% calcium hydroxide regulation pH to
Neutrality, is then extracted with dichloromethane, is extracted 3~5 times repeatedly, is reclaimed dichloromethane, is obtained leaf of Moringa total alkaloid.
7. any Moringa hypoglycemic activity composition is preparing pressed candy, oral administration effervescing in claim 1 to 6
Application in piece, lozenge, capsule.
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| CN107495117A (en) * | 2017-09-15 | 2017-12-22 | 广东日可威食品原料有限公司 | A kind of hypotensive jujube pumpkin cake and preparation method thereof |
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Application publication date: 20170728 |