CN106983033A - The preparation method of one breeder health-care feed - Google Patents

The preparation method of one breeder health-care feed Download PDF

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Publication number
CN106983033A
CN106983033A CN201710193342.3A CN201710193342A CN106983033A CN 106983033 A CN106983033 A CN 106983033A CN 201710193342 A CN201710193342 A CN 201710193342A CN 106983033 A CN106983033 A CN 106983033A
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fermentation
feed
chicken
solid state
health
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阮晖
邱亦亦
金迅
丁丽娜
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Zhejiang University ZJU
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Zhejiang University ZJU
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
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    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention discloses the preparation method of a breeder health-care feed, this method comprises the following steps:(1) Radix Astragali, radix glycyrrhizae, dried orange peel are well mixed with chicken basal feed, then add lees of yellow wine and linden black fungus bacterial rod thereto, obtain mixture;(2) mixture is carried out after solid state fermentation, fermentation ends, size-reduced, granulation obtains chicken health-care feed.The present invention in basal feed by adding the Radix Astragali, radix glycyrrhizae and dried orange peel, and be that leavening and fermentation substrate carry out solid state fermentation using lees of yellow wine and linden black fungus bacterial rod, obtain with rich in both effectiveness active material, the chicken health-care feed that cellulosic enzyme content is high, palatability is good, safe, significantly improve the food ration of chicken, and promote chicken to eliminate sub-health state, and health status is turned to by sub-health state, so as to significantly improve the production performance of chicken.

Description

The preparation method of one breeder health-care feed
Technical field
The present invention relates to herding field, and in particular to the preparation method of a breeder health-care feed.
Background technology
Animal is lived in natural environment, and the moment can be attacked by various virulence factors, and animal body also per when it is every It is engraved in transfer disease resistance mechanisms.Therefore, animal definitely health is minority, in most cases in sub-health state.Health is supported It is animal husbandry development trend from now on to grow, and feed of the research and development with health-care efficacy is to carry out animal health cultivation, promote animal physiological State is turned to the effective way of health by inferior health.
Health-care feed is also known as functional feed, and the health food or healthy food of similar people with raising immunity, are adjusted Reason intestinal physiology, resisting stress, promotion are ingested and grown etc. specific function.Chinese medicine rich in both effectiveness natural products is that health care is raised The important sources of both effectiveness composition in material.
At present, the research on chicken health-care feed is too numerous to enumerate, for example:
Application publication number discloses a breeder health-care feed, the raw material of the feed for CN101194681 patent of invention And parts by weight are:50-60 parts of corn, 10-15 parts of dregs of beans, 5-10 parts of rapeseed meal, 5-14 parts of wheat bran, 5-14 parts of rice bran, stone flour 2-5 Part, 0.1-1.0 parts of methionine, 0.3-0.5 parts of salt, 0.4-0.8 parts of cactus dry powder, 0.3-0.7 parts of aloe dry powder, garlic dry powder 0.1-0.3 parts, 0.3-0.5 parts of honey.Its preparation method is:First particulate material, block in above-mentioned raw materials are crushed, then by each original Material, which is placed into after being weighed by ratio requirement in mixer, to carry out being sufficiently stirred for mixing i.e. finished product.
Application publication number discloses a kind of laying hen health-care feed for CN104738313A application for a patent for invention, the feed by The raw material of following parts by weight is made:Buckwheat 60-70, wheat bran 30-40, soyabean protein powder 5-10, bittern 4-7, flower glue 5-7, material Wine 2-3, butter 3-4, red bean powder 3-6, Lupinus albus powder 6-10, shelled peanut 5-10, jujube 8-12, pea leaf 12-15, bamboo charcoal powder 1-2, pumpkin cauline leaf 20-25, sponge gourd cauline leaf 10-15, fennel spray 8-14, lepidium seed 1-2, honeysuckle 1-2, dandelion 2-3, Huang Mealworm excrement 20-25, white sugar 5-6, saccharomycete 3-4, phagostimulant 3-4, appropriate amount of water.
The Radix Astragali has the work of enhancing body's immunity, liver protection, diuresis, anti-aging, resisting stress, decompression and extensive antibacterial With.Radix glycyrrhizae is a kind of help Chinese herbal medicine, and major function is clearing heat and detoxicating, anti-inflammatory, antiallergy, expelling phlegm and arresting coughing, gastral cavity abdomen etc..Dried orange peel has Regulating qi-flowing for strengthening spleen, it is eliminating dampness and eliminating phlegm, neutralizing stagnation of QI due to dyspepsia the effects such as.
Except optimization addition Chinese medicine, microbial fermentation be to confer to feed healthcare function, abatement feed in unsafe factor, Lift the important channel of feed quality.There are many advantages in terms of operability, cost, cost performance in solid state fermentation.
Healthy aquaculture is animal husbandry development trend from now on, therefore, and research and development health-care feed is the pass for carrying out animal health cultivation Key link.
The content of the invention
The invention provides the preparation method of a breeder health-care feed, chicken health-care feed prepared by this method is not only rich in work( Effect property active material, cellulosic enzyme content are high, palatability is good, safe, and can improve food ration and its production of chicken Performance.
The preparation method of one breeder health-care feed, comprises the following steps:
(1) Radix Astragali, radix glycyrrhizae, dried orange peel are well mixed with chicken basal feed, then addition lees of yellow wine and linden are black thereto Agaric fungus rod, obtains mixture;
(2) mixture is carried out after solid state fermentation, fermentation ends, size-reduced, granulation obtains chicken health-care feed.
The above-mentioned Radix Astragali has enhancing body's immunity, liver protection, diuresis, anti-aging, resisting stress, decompression and extensive antibacterial Effect;Radix glycyrrhizae cures mainly clearing heat and detoxicating, anti-inflammatory, antiallergy, expelling phlegm and arresting coughing, gastral cavity abdomen etc.;And dried orange peel has regulating qi-flowing for strengthening spleen, eliminating dampness The effects such as phlegm, neutralizing stagnation of QI due to dyspepsia.
The Radix Astragali, radix glycyrrhizae, dried orange peel are ground into 1~2cm length or 1cm × 1cm square, then mixed with chicken basal feed Close;The linden black fungus bacterial rod is also ground into 1~2cm length or 1cm × 1cm square.
Lees of yellow wine is with the accessory substance after starchy material fermentation yellow rice wine.Contain high density yeast in lees of yellow wine, with And the nutriment not being fully used, including most albumen, fat, calcium, phosphorus etc., and its abundant yeast fermentation of species Product.Lees of yellow wine, which is added in feed, can breed the yeast in feed and cut down the mould in feed, produce what is enriched Both effectiveness material, makes feed integrally have vinasse local flavor, so as to improve the palatability of feed, promotes animal ingestion, and lift feeding Item matter, guarantee safety.
Above-mentioned linden black fungus bacterial rod refers to the bacterium rod discarded after cultivation linden black fungus, and it is rich in a large amount of black fungus spores And its cellulosic enzyme of secretion, the linden black fungus bacterial rod after black fungus is harvested crushes and is added in feed, Neng Goucheng For a kind of enzyme preparation, during feed fermentation, enzyme activity can be dramatically increased.
Experiment is found, lees of yellow wine is added into chicken basal feed and linden black fungus bacterial rod carries out solid state fermentation, not only Unemployed nutriment in vinasse and linden black fungus bacterial rod can effectively be utilized, additionally it is possible to preferably decompose release yellow Nutritional ingredient in stilbene, radix glycyrrhizae, dried orange peel, improves the food ration of chicken, and then improve the production performance of chicken.
Preferably, in terms of 1000kg chicken basal feed, the Radix Astragali, radix glycyrrhizae, the addition of dried orange peel are followed successively by:15~ 20kg, 15~20kg, 15~20kg.
It is further preferred that in terms of 1000kg chicken basal feed, the Radix Astragali, radix glycyrrhizae, the addition of dried orange peel are followed successively by:17~ 18kg, 17~18kg, 17~18kg.
The selection of concrete component and consumption not only influence the pharmacological effect of the Radix Astragali, radix glycyrrhizae, dried orange peel in chicken basal feed, equally Also solid state fermentation effect can be influenceed.
Preferably, the component of the chicken basal feed is:
Lees of yellow wine can increase the yeast quantity in feed, and eliminate mould in feed as leavening in fermentation Bacterium, improves feed quality and security.Preferably, the addition of the lees of yellow wine is 50~55kg, ferment in lees of yellow wine Female density >=109cfu/g.Enough yeast initial densities are to ensure one of key factor that solid state fermentation is successfully carried out.
Linden black fungus bacterial rod is bred as leavening, contained black fungus spore in solid ferment process, can be improved Feed cellulase vigor.Preferably, the linden black fungus bacterial rod should be the bacterium rod discarded after plantation black fungus harvest, Addition is 50~55kg.The black fungus spore High Cellulase Production being rich in obsolete fungus stick.
Further, in step (2), the temperature of the solid state fermentation is 24~32 DEG C, and the time is 45~50h, and every 12h stirrings are once.Suitable temperature and enough fermentation times, and timing stirring have sufficient oxygen to ensure inside feed Supply, it is ensured that solid state fermentation is successfully carried out.
Further, in step (2), the laying depth of the mixture is 4~6cm, beneficial to solid state fermentation, reduces mould Quantity.Suitable material thickness can ensure there is sufficient oxygen supply inside feed, too thin uneconomical, too thick, hypoventilation and lead Cause feed internal oxygen insufficient.
Preferably, in step (2), water is added before solid state fermentation into mixture, the addition of the water is mixture The 20~30% of quality.Necessary to suitable water is Yeast proliferation, water shortage causes Yeast proliferation speed slow, water mistake At most feed porosity is not enough so as to which oxygen is not enough, again results in Yeast proliferation speed slowly, and miscellaneous bacteria easily pollutes.
Preferably, in step (2), the temperature of the granulation is 75~85 DEG C.Feed is cured at this temperature, and chicken is taken the photograph Nutritional utilization is more abundant during food, moreover it is possible to play pasteurization.
Compared with prior art, the invention has the advantages that:
(1) it is of the invention by adding the Radix Astragali, radix glycyrrhizae and dried orange peel in basal feed, and with lees of yellow wine and linden black fungus Bacterium rod is that leavening carries out solid state fermentation, is obtained with rich in both effectiveness active material, cellulosic enzyme content height, palatability Good, safe chicken health-care feed, significantly improves the food ration of chicken, and promotes chicken to eliminate sub-health state, by inferior health State shift health status, so as to significantly improve the production performance of chicken.
(2) preparation method of the present invention is simple, and the product quality prepared is high, beneficial to popularization.
Embodiment
In following examples and comparative example, if without specified otherwise, the Radix Astragali, radix glycyrrhizae and dried orange peel are conventional medicinal materials, are Ordinary commercial products.
Linden black fungus bacterial rod is used for the following example to cultivate the bacterium rod discarded after linden black fungus after size-reduced processing In.After linden black fungus bacterial rod is planted black fungus and harvested, wherein rich in black fungus spore, the spore High Cellulase Production.
Mould and yeast counts assay method use " national food safety standard-food microbiological examination-mould and ferment Mother counts (GB 4789.15-2016) "
Cellulose enzyme vigor assay method is using " the measure filter paper method of national proposed standard-feeding cellulase activity (GB/T 23881-2009)”。
Embodiment 1
The preparation method of one breeder health-care feed, is comprised the following steps that:
(1) by chicken basal feed (in terms of per ton, corn 200kg;Crack rice 150kg;Wheat 100kg;Soya-bean cake 260kg;Broken green grass or young crops Expect 200kg;Fish meal 30kg;Bone meal 30kg;Crude salt 5kg) be well mixed with Radix Astragali 15kg, radix glycyrrhizae 15kg, dried orange peel 15kg, then to its Middle addition yeast density is higher than 109Cfu/g lees of yellow wine 50kg, linden black fungus bacterial rod 50kg, obtains mixture;
(2) the indoor water mire that mixture is placed in after running water is rinsed well, carries out solid state fermentation;Indoor temperature is 24 DEG C, fermentation material thickness be 4cm, surface uniform sprinkling to the 20% of fermentation material gross mass, fermentation time is 48h, is turned over every 12h Material is once;
(3) it is size-reduced, ring die pelleter is pelletized at 75 DEG C, obtains chicken health-care feed after solid state fermentation terminates.
Before and after measure solid state fermentation, the quantity of yeast and mould, cellulase activity in fermentation material;And subjective appreciation ferments Front and rear feed quality.
As a result it is as follows:
Before solid state fermentation, treat to contain 1.26 × 10 in fermentation raw material7Cfu/g yeast, 4.28 × 107Cfu/g mould, Cellulase activity is 52.5U/g;After solid state fermentation, 1.74 × 10 are contained in fermentation material8Cfu/g yeast, 6.33 × 106Cfu/g mould, cellulase activity is 345.8U/g;I.e.:Before and after solid state fermentation, Yeast proliferation is 13.81 before fermentation Times, and mould abatement is 14.79% before fermentation, cellulase activity is 6.59 times before fermentation.
The chicken health-care feed without vinasse local flavor, has vinasse local flavor before solid state fermentation after solid state fermentation.
Feeding experiment:
Sanhuang chicken (35 age in days) 40 is selected, 2 groups are bisected at random, test group feeds the chicken guarantor that the present embodiment is prepared Strong feed, the chicken basal feed described in control group fed the present embodiment, feeding experiment, 70 ages in days experiment knot since 35 ages in days Beam.
Concrete outcome see the table below:
Group Starting weight (kg) It is heavy (kg) eventually Daily gain (g) Daily ingestion amount (g) Feed-weight ratio
Test group 410±15.1 833±22.3* 12.1±1.3* 38.7 3.20
Control group 415±16.5 802±12.8 11.1±0.8 37.2 3.35
*:With control group significant difference, p<0.05
The above results are shown, compared with control group, and test group brings up again high 3.87% (p eventually<0.05), daily gain is improved 9.01% (p<0.05), daily ingestion amount improves 4.03% (p<0.05), feed-weight ratio improves 4.48%.
Embodiment 2
The preparation method of one breeder health-care feed, is comprised the following steps that:
(1) by chicken basal feed (in terms of per ton, corn 200kg;Crack rice 150kg;Wheat 100kg;Soya-bean cake 260kg;Broken green grass or young crops Expect 200kg;Fish meal 30kg;Bone meal 30kg;Crude salt 5kg) be well mixed with Radix Astragali 20kg, radix glycyrrhizae 20kg, dried orange peel 20kg, then to its Middle addition yeast density is higher than 109Cfu/g lees of yellow wine 55kg, linden black fungus bacterial rod 55kg, obtains mixture;
(2) the indoor water mire that mixture is placed in after running water is rinsed well, carries out solid state fermentation;Indoor temperature is 32 DEG C, fermentation material thickness be 6cm, surface uniform sprinkling to the 30% of fermentation material gross mass, fermentation time is 48h, is turned over every 12h Material is once;
(3) it is size-reduced, ring die pelleter is pelletized at 75 DEG C, obtains chicken health-care feed after solid state fermentation terminates.
Before and after measure solid state fermentation, the quantity of yeast and mould, cellulase activity in fermentation material;And subjective appreciation ferments Front and rear feed quality.
As a result it is as follows:
Before solid state fermentation, treat to contain 2.14 × 10 in fermentation raw material7Cfu/g yeast, 5.75 × 107Cfu/g mould, Cellulase activity is 58.0;After solid state fermentation, 4.06 × 10 are contained in fermentation material8Cfu/g yeast, 7.42 × 106cfu/g Mould, cellulase activity is 477.8U/g;I.e.:Before and after solid state fermentation, Yeast proliferation is 18.97 times before fermentation, and mould Bacterium abatement is 12.90% before fermentation, and cellulase activity is 8.24 times before fermentation.
The chicken health-care feed without vinasse local flavor, has vinasse local flavor before solid state fermentation after solid state fermentation.
Feeding experiment:
Sanhuang chicken (35 age in days) 40 is selected, 2 groups are bisected at random, test group feeds the chicken guarantor that the present embodiment is prepared Strong feed, the chicken basal feed described in control group fed the present embodiment, feeding experiment, 70 ages in days experiment knot since 35 ages in days Beam.
Concrete outcome see the table below:
Group Starting weight (kg) It is heavy (kg) eventually Daily gain (g) Daily ingestion amount (g) Feed-weight ratio
Test group 422±10.5 863±24.2* 12.6±1.2* 40.07 3.18
Control group 426±18.0 818±19.2 11.2±0.9 37.41 3.34
*:With control group significant difference, p<0.05
The above results are shown, compared with control group, and test group brings up again high 5.50% (p eventually<0.05), daily gain is improved 12.50% (p<0.05), daily ingestion amount improves 7.11% (p<0.05), feed-weight ratio improves 4.49%.
Embodiment 3
The preparation method of one breeder health-care feed, is comprised the following steps that:
(1) by chicken basal feed (in terms of per ton, corn 200kg;Crack rice 150kg;Wheat 100kg;Soya-bean cake 260kg;Broken green grass or young crops Expect 200kg;Fish meal 30kg;Bone meal 30kg;Crude salt 5kg) be well mixed with Radix Astragali 17kg, radix glycyrrhizae 17kg, dried orange peel 17kg, then to its Middle addition yeast density is higher than 109Cfu/g lees of yellow wine 52kg, linden black fungus bacterial rod 52kg, obtains mixture;
(2) the indoor water mire that mixture is placed in after running water is rinsed well, carries out solid state fermentation;Indoor temperature is 28 DEG C, fermentation material thickness be 5cm, surface uniform sprinkling to the 25% of fermentation material gross mass, fermentation time is 48h, is turned over every 12h Material is once;
(3) it is size-reduced, ring die pelleter is pelletized at 75 DEG C, obtains chicken health-care feed after solid state fermentation terminates.
Before and after measure solid state fermentation, the quantity of yeast and mould, cellulase activity in fermentation material;And subjective appreciation ferments Front and rear feed quality.
As a result it is as follows:
Before solid state fermentation, treat to contain 2.35 × 10 in fermentation raw material7Cfu/g yeast, 3.78 × 107Cfu/g mould, Cellulase activity is 42.7U/g;After solid state fermentation, 4.88 × 10 are contained in fermentation material8Cfu/g yeast, 3.28 × 106Cfu/g mould, cellulase activity is 584.6U/g;I.e.:Before and after solid state fermentation, Yeast proliferation is 20.77 before fermentation Times, and mould abatement is 8.68% before fermentation, cellulase activity is 13.69 times before fermentation.
The chicken health-care feed without vinasse local flavor, has vinasse local flavor before solid state fermentation after solid state fermentation.
Feeding experiment:
Sanhuang chicken (35 age in days) 40 is selected, 2 groups are bisected at random, test group feeds the chicken guarantor that the present embodiment is prepared Strong feed, the chicken basal feed described in control group fed the present embodiment, feeding experiment, 70 ages in days experiment knot since 35 ages in days Beam.
Concrete outcome see the table below:
Group Starting weight (kg) It is heavy (kg) eventually Daily gain (g) Daily ingestion amount (g) Feed-weight ratio
Test group 417±15.5 886±20.0* 13.4±1.3* 42.21 3.15
Control group 420±10.6 829±13.3 11.7±1.0 38.84 3.32
*:With control group significant difference, p<0.05
The above results are shown, compared with control group, and test group brings up again high 6.88% (p eventually<0.05), daily gain is improved 14.53% (p<0.05), daily ingestion amount improves 8.68% (p<0.05), feed-weight ratio improves 5.12%.
Embodiment 4
The preparation method of one breeder health-care feed, is comprised the following steps that:
(1) by chicken basal feed (in terms of per ton, corn 200kg;Crack rice 150kg;Wheat 100kg;Soya-bean cake 260kg;Broken green grass or young crops Expect 200kg;Fish meal 30kg;Bone meal 30kg;Crude salt 5kg) be well mixed with Radix Astragali 18kg, radix glycyrrhizae 18kg, dried orange peel 18kg, then to its Middle addition yeast density is higher than 109Cfu/g lees of yellow wine 53kg, linden black fungus bacterial rod 53kg, obtains mixture;
(2) by the indoor water mire being placed in mixture after running water is rinsed well, solid state fermentation is carried out;Indoor temperature For 28 DEG C, fermentation material thickness is 5cm, surface uniform sprinkling to the 25% of fermentation material gross mass, and fermentation time is 48h, every 12h Stirring is once;
(3) it is size-reduced, ring die pelleter is pelletized at 75 DEG C, obtains chicken health-care feed after solid state fermentation terminates.
Before and after measure solid state fermentation, the quantity of yeast and mould, cellulase activity in fermentation material;And subjective appreciation ferments Front and rear feed quality.
As a result it is as follows:
Before solid state fermentation, treat to contain 2.48 × 10 in fermentation raw material7Cfu/g yeast, 4.20 × 107Cfu/g mould, Cellulase activity is 44.3U/g;After solid state fermentation, 5.13 × 10 are contained in fermentation material8Cfu/g yeast, 2.84 × 106Cfu/g mould, cellulase activity is 590.7U/g;I.e.:Before and after solid state fermentation, Yeast proliferation is 20.68 before fermentation Times, and mould abatement is 6.76% before fermentation, cellulase activity is 13.33 times before fermentation.
The chicken health-care feed without vinasse local flavor, has vinasse local flavor before solid state fermentation after solid state fermentation.
Feeding experiment:
Sanhuang chicken (35 age in days) 40 is selected, 2 groups are bisected at random, test group feeds the chicken guarantor that the present embodiment is prepared Strong feed, the chicken basal feed described in control group fed the present embodiment, feeding experiment, 70 ages in days experiment knot since 35 ages in days Beam.
Concrete outcome see the table below:
Group Starting weight (kg) It is heavy (kg) eventually Daily gain (g) Daily ingestion amount (g) Feed-weight ratio
Test group 414±10.2 862±17.7* 12.8±1.6* 40.45 3.16
Control group 410±8.4 805±14.5 11.3±1.0 37.63 3.33
*:With control group significant difference, p<0.05
The above results are shown, compared with control group, and test group brings up again high 7.11% (p eventually<0.05), daily gain is improved 13.27% (p<0.05), daily ingestion amount improves 7.49% (p<0.05), feed-weight ratio improves 5.11%.
Comparative example 1
The comparative example compared with Example 1, lacks a kind of component of the Radix Astragali.
The preparation method of one breeder health-care feed, is comprised the following steps that:
(1) by chicken basal feed (in terms of per ton, corn 200kg;Crack rice 150kg;Wheat 100kg;Soya-bean cake 260kg;Broken green grass or young crops Expect 200kg;Fish meal 30kg;Bone meal 30kg;Crude salt 5kg) it is well mixed with radix glycyrrhizae 15kg, dried orange peel 15kg, then ferment is added thereto Female density is higher than 109Cfu/g lees of yellow wine 50kg, linden black fungus bacterial rod 50kg, obtain mixture;
(2) solid ferment process be the same as Example 1;
(3) pelletization be the same as Example 1.
Before and after measure solid state fermentation, the quantity of yeast and mould, cellulase activity in fermentation material;And subjective appreciation ferments Front and rear feed quality.
As a result it is as follows:
Before solid state fermentation, treat to contain 5.13 × 10 in fermentation raw material7Cfu/g yeast, 9.36 × 107Cfu/g mould; After solid state fermentation, 2.10 × 10 are contained in fermentation material8Cfu/g yeast, 3.89 × 107Cfu/g mould;I.e.:Before solid state fermentation Afterwards, Yeast proliferation is 4.09 times before fermentation, and mould abatement is 41.56% before fermentation.
Before solid state fermentation, cellulase activity is 53.1U/g;After solid state fermentation, cellulase activity is 213.5U/g, is increased Add as 4.02 times before fermentation.
The chicken health-care feed without vinasse local flavor, has vinasse local flavor before solid state fermentation after solid state fermentation.
Feeding experiment:
Sanhuang chicken (35 age in days) 40 is selected, 2 groups are bisected at random, test group feeds the chicken guarantor that the present embodiment is prepared Strong feed, the chicken basal feed described in control group fed the present embodiment, feeding experiment, 70 ages in days experiment knot since 35 ages in days Beam.
Concrete outcome see the table below:
Group Starting weight (kg) It is heavy (kg) eventually Daily gain (g) Daily ingestion amount (g) Feed-weight ratio
Test group 407±7.8 815±11.7* 11.7±1.5 37.9 3.24
Control group 408±6.5 798±8.2 11.1±1.1 37.3 3.36
*:With control group significant difference, p<0.05
The above results are shown, compared with control group, and test group brings up again high 2.13% (p eventually<0.05), daily gain is improved 5.41%, daily ingestion amount improves 1.61%, and feed-weight ratio improves 2.38%.
Compared with Example 1, the chicken health-care feed prepared by the comparative example still has vinasse local flavor, and yeast and mould are also divided Substantially do not bred and cut down, culture efficiency is slightly excellent compared with the control, but be far inferior to embodiment 1, illustrate the missing Radix Astragali to chicken Effect of health-care feed has a significant effect.
Comparative example 2
The comparative example compared with Example 1, lacks a kind of component of dried orange peel.
The preparation method of one breeder health-care feed, is comprised the following steps that:
(1) by chicken basal feed (in terms of per ton, corn 200kg;Crack rice 150kg;Wheat 100kg;Soya-bean cake 260kg;Broken green grass or young crops Expect 200kg;Fish meal 30kg;Bone meal 30kg;Crude salt 5kg) it is well mixed with Radix Astragali 15kg, radix glycyrrhizae 15kg, then ferment is added thereto Female density is higher than 109Cfu/g lees of yellow wine 50kg, linden black fungus bacterial rod 50kg, obtain mixture;
(2) solid ferment process be the same as Example 1;
(3) pelletization be the same as Example 1.
Before and after measure solid state fermentation, the quantity of yeast and mould, cellulase activity in fermentation material;And subjective appreciation ferments Front and rear feed quality.
As a result it is as follows:
Before solid state fermentation, treat to contain 5.65 × 10 in fermentation raw material7Cfu/g yeast, 8.45 × 107Cfu/g mould; After solid state fermentation, 2.32 × 10 are contained in fermentation material8Cfu/g yeast, 3.60 × 107Cfu/g mould;I.e.:Before solid state fermentation Afterwards, Yeast proliferation is 4.11 times before fermentation, and mould abatement is 42.60% before fermentation.
Before solid state fermentation, cellulase activity is 47.4U/g;After solid state fermentation, cellulase activity is 182.5U/g, is increased Add as 3.85 times before fermentation.
The chicken health-care feed without vinasse local flavor, has vinasse local flavor before solid state fermentation after solid state fermentation.
Feeding experiment:
Sanhuang chicken (35 age in days) 40 is selected, 2 groups are bisected at random, test group feeds the chicken guarantor that the present embodiment is prepared Strong feed, the chicken basal feed described in control group fed the present embodiment, feeding experiment, 70 ages in days experiment knot since 35 ages in days Beam.
Concrete outcome see the table below:
Group Starting weight (kg) It is heavy (kg) eventually Daily gain (g) Daily ingestion amount (g) Feed-weight ratio
Test group 414±12.8 819±21.7 11.6±1.5 38.1 3.28
Control group 410±10.5 801±15.2 11.2±1.1 37.7 3.37
With control group without significant difference.
The above results are shown, compared with control group, and test group brings up again high by 2.25% eventually, and daily gain improves 3.57%, and day adopts Appetite improves 1.06%, and feed-weight ratio improves 2.67%.
Compared with Example 1, the chicken health-care feed prepared by the comparative example still has vinasse local flavor, and yeast and mould are also divided Substantially do not bred and cut down, culture efficiency is slightly excellent compared with the control, but be far inferior to embodiment 1, illustrate missing dried orange peel to chicken Effect of health-care feed has a significant effect.
Comparative example 3
The comparative example compared with Example 1, lacks in two kinds of components of fish meal and crude salt, chicken basal feed in chicken basal feed Corn amount 235kg is increased to by 200kg.
The preparation method of one breeder health-care feed, is comprised the following steps that:
(1) by chicken basal feed (in terms of per ton, corn 235kg;Crack rice 150kg;Wheat 100kg;Soya-bean cake 260kg;Broken green grass or young crops Expect 200kg;Bone meal 30kg) it is well mixed with Radix Astragali 15kg, radix glycyrrhizae 15kg, dried orange peel 15kg, then addition yeast density is high thereto In 109Cfu/g lees of yellow wine 50kg, linden black fungus bacterial rod 50kg, obtain mixture;
(2) solid ferment process be the same as Example 1;
(3) pelletization be the same as Example 1.
Before and after measure solid state fermentation, the quantity of yeast and mould, cellulase activity in fermentation material;And subjective appreciation ferments Front and rear feed quality.
As a result it is as follows:
Before solid state fermentation, treat to contain 5.65 × 10 in fermentation raw material7Cfu/g yeast, 8.45 × 107Cfu/g mould; After solid state fermentation, 2.32 × 10 are contained in fermentation material8Cfu/g yeast, 3.60 × 107Cfu/g mould;I.e.:Before solid state fermentation Afterwards, Yeast proliferation is 4.11 times before fermentation, and mould abatement is 42.60% before fermentation.
Before solid state fermentation, cellulase activity is 50.5U/g;After solid state fermentation, cellulase activity is 198.8U/g, is increased Add as 3.94 times before fermentation.
The chicken health-care feed without vinasse local flavor, has vinasse local flavor before solid state fermentation after solid state fermentation.
Feeding experiment:
Sanhuang chicken (35 age in days) 40 is selected, 2 groups are bisected at random, test group feeds the chicken guarantor that the present embodiment is prepared Strong feed, the chicken basal feed described in control group fed the present embodiment, feeding experiment, 70 ages in days experiment knot since 35 ages in days Beam.
Concrete outcome see the table below:
Group Starting weight (kg) It is heavy (kg) eventually Daily gain (g) Daily ingestion amount (g) Feed-weight ratio
Test group 412±9.5 815±7.4* 11.5±0.9 37.6 3.27
Control group 408±6.2 795±11.7 11.1±1.1 37.3 3.36
*:With control group significant difference, p<0.05
The above results are shown, compared with control group, and test group brings up again high 2.52% (p eventually<0.05), daily gain is improved 3.60%, daily ingestion amount improves 0.80%, and feed-weight ratio improves 2.68%.
Compared with Example 1, the chicken health-care feed prepared by the comparative example still has vinasse local flavor, and yeast and mould are also divided Substantially do not bred and cut down, culture efficiency is slightly excellent compared with the control, but be far inferior to embodiment 1, illustrate missing fish meal and thick Two kinds of components of salt have a significant effect to effect of chicken health-care feed.
Comparative example 4
Compared with Example 1, unused lees of yellow wine ferments the comparative example, and uses nature solid state fermentation.
The preparation method of one breeder health-care feed, is comprised the following steps that:
(1) lees of yellow wine, other feedstuffs and component be the same as Example 1 are free of;
(2) the indoor water mire that mixture (no lees of yellow wine) is placed in after running water is rinsed well, carries out nature and consolidates State is fermented, fermentation process be the same as Example 1;
(3) pelletization be the same as Example 1.
Before and after measure solid state fermentation, the quantity of yeast and mould, cellulase activity in fermentation material;And subjective appreciation ferments Front and rear feed quality.
As a result it is as follows:
Before solid state fermentation, treat to contain 4.80 × 10 in fermentation raw material7Cfu/g yeast, 5.22 × 107Cfu/g mould; After solid state fermentation, 3.25 × 10 are contained in fermentation material7Cfu/g yeast, 6.74 × 109Cfu/g mould;I.e.:Natural solid-state hair After ferment, yeast is 67.71% before fermentation, and mould is 129 times before fermentation.
Before solid state fermentation, cellulase activity is 65.3U/g;After solid state fermentation, cellulase activity is 78.2U/g, is increased Add as 1.20 times before fermentation.
The chicken health-care feed without vinasse local flavor, has the taste that substantially goes mouldy before natural solid state fermentation after natural solid state fermentation.
Feeding experiment:
Sanhuang chicken (35 age in days) 40 is selected, 2 groups are bisected at random, test group feeds the chicken guarantor that the present embodiment is prepared Strong feed, the chicken basal feed described in control group fed the present embodiment, feeding experiment, 70 ages in days experiment knot since 35 ages in days Beam.
Concrete outcome see the table below:
Group Starting weight (kg) It is heavy (kg) eventually Daily gain (g) Daily ingestion amount (g) Feed-weight ratio
Test group 424±11.5 763±9.4* 9.7±1.0* 33.5 3.45
Control group 420±11.2 806±7.7 11.0±0.9 37.0 3.36
*:With control group significant difference, p<0.05
The above results are shown, compared with control group, and test group declines 5.33% (p again eventually<0.05), daily gain declines 11.82% (p<0.05), daily ingestion amount declines 9.46%, and feed-weight ratio deteriorates 2.68%.
Compared with Example 1, the chicken health-care feed prepared by the comparative example, which has, substantially goes mouldy, mould density extremely significantly on Rise, it is unsuccessful illustrate nature solid state fermentation, cause feed mold go bad, culture efficiency substantially compare take a picture it is poor, it is farther inferior In embodiment 1.
Comparative example 5
The comparative example compared with Example 1, is not fermented using lees of yellow wine, and uses fermentation by saccharomyces cerevisiae.
The preparation method of one breeder health-care feed, is comprised the following steps that:
(1) lees of yellow wine is free of, and instead density is higher than 109Cfu/g saccharomyces cerevisiae nutrient solution 50L, other feeds Raw material and component be the same as Example 1;
(2) the indoor water mire that mixture is placed in after running water is rinsed well, carries out nature solid state fermentation, fermented Journey be the same as Example 1;
(3) pelletization be the same as Example 1.
Before and after measure solid state fermentation, the quantity of yeast and mould, cellulase activity in fermentation material;And subjective appreciation ferments Front and rear feed quality.
As a result it is as follows:
Before solid state fermentation, treat to contain 3.85 × 10 in fermentation raw material7Cfu/g yeast, 6.61 × 107Cfu/g mould; After solid state fermentation, 4.59 × 10 are contained in fermentation material7Cfu/g yeast, 2.83 × 108Cfu/g mould;I.e.:Before solid state fermentation Afterwards, Yeast proliferation is 1.19 times before fermentation, and mould propagation is 4.28 times before fermentation.
Before solid state fermentation, cellulase activity is 60.6U/g;After solid state fermentation, cellulase activity is 115.2U/g, is increased Add as 1.90 times before fermentation.
The chicken health-care feed before solid state fermentation without vinasse local flavor, without vinasse or other ferment local-flavors after solid state fermentation.
Feeding experiment:
Sanhuang chicken (35 age in days) 40 is selected, 2 groups are bisected at random, test group feeds the chicken guarantor that the present embodiment is prepared Strong feed, the chicken basal feed described in control group fed the present embodiment, feeding experiment, 70 ages in days experiment knot since 35 ages in days Beam.
Concrete outcome see the table below:
Group Starting weight (kg) It is heavy (kg) eventually Daily gain (g) Daily ingestion amount (g) Feed-weight ratio
Test group 405±7.6 805±10.5 11.4±1.2 38.2 3.35
Control group 404±9.2 801±10.2 11.3±1.1 38.0 3.36
With control group without significant difference.
The above results show that test group brings up again high by 0.50% eventually, and daily gain improves 0.91%, and daily ingestion amount is improved 0.53%, feed-weight ratio improves 0.30%.
Compared with Example 1, the chicken health-care feed prepared by the comparative example is without obvious ferment local-flavor, and yeast and mould are not yet Fully bred and cut down, illustrated in solid-state environment, carried out with yeast merely fermentation can not Effective multiplication, mould can not yet It is effectively suppressed, culture efficiency basic indifference compared with the control is far inferior to embodiment 1, illustrates to substitute yellow rice wine wine with yeast It is unsuccessful that grain carries out solid state fermentation.
Comparative example 6
The comparative example compared with Example 1, is not fermented using lees of yellow wine, and uses " Daqu " white spirit vinasse fermentation.
The preparation method of one breeder health-care feed, is comprised the following steps that:
(1) without lees of yellow wine, and instead " Daqu " white spirit vinasse 50kg, other feedstuffs and component be the same as Example 1;
(2) the indoor water mire that mixture is placed in after running water is rinsed well, carries out nature solid state fermentation, fermented Journey be the same as Example 1;
(3) pelletization be the same as Example 1.
Before and after measure solid state fermentation, the quantity of yeast and mould, cellulase activity in fermentation material;And subjective appreciation ferments Front and rear feed quality.
As a result it is as follows:
Before solid state fermentation, treat to contain 2.28 × 10 in fermentation raw material7Cfu/g yeast, 3.15 × 107Cfu/g mould; After solid state fermentation, 1.65 × 10 are contained in fermentation material8Cfu/g yeast, 7.20 × 106Cfu/g mould;I.e.:Before solid state fermentation Afterwards, Yeast proliferation is 7.24 times before fermentation, and mould abatement is 22.86% before fermentation.
Before solid state fermentation, cellulase activity is 51.0U/g;After solid state fermentation, cellulase activity is 142.5U/g, is increased Add as 2.79 times before fermentation.
The chicken health-care feed without vinasse local flavor, has vinasse local flavor before solid state fermentation after solid state fermentation.
Feeding experiment:
Sanhuang chicken (35 age in days) 40 is selected, 2 groups are bisected at random, test group feeds the chicken guarantor that the present embodiment is prepared Strong feed, the chicken basal feed described in control group fed the present embodiment, feeding experiment, 70 ages in days experiment knot since 35 ages in days Beam.
Concrete outcome see the table below:
Group Starting weight (kg) It is heavy (kg) eventually Daily gain (g) Daily ingestion amount (g) Feed-weight ratio
Test group 412±9.5 815±7.4* 11.5±0.9 37.6 3.27
Control group 408±6.2 795±11.7 11.1±1.1 37.3 3.36
*:With control group significant difference, p<0.05
The above results show that test group brings up again high 2.52% (p eventually<0.05), daily gain improves 3.60%, and daily ingestion amount is carried High by 0.80%, feed-weight ratio improves 2.68%.
Compared with Example 1, the chicken health-care feed prepared by the comparative example has ferment local-flavor, and yeast and mould are increased Grow and cut down, culture efficiency has a clear superiority compared with the control, but be far inferior to embodiment 1, illustrate to carry out with " Daqu " white spirit vinasse Fermentation is also feasible, but effect is not so good as lees of yellow wine.
Comparative example 7
The comparative example lacks linden black fungus bacterial rod, remaining component and fermentation process compared with Example 1, in feed ingredient All same.
The preparation method of one breeder health-care feed, is comprised the following steps that:
(1) linden black fungus bacterial rod, other feedstuffs and component be the same as Example 1 are free of;
(2) the indoor water mire that mixture is placed in after running water is rinsed well, carries out nature solid state fermentation, fermented Journey be the same as Example 1;
(3) pelletization be the same as Example 1.
Before and after measure solid state fermentation, the quantity of yeast and mould, cellulase activity in fermentation material;And subjective appreciation ferments Front and rear feed quality.
As a result it is as follows:
Before solid state fermentation, treat to contain 4.55 × 10 in fermentation raw material7Cfu/g yeast, 5.17 × 107Cfu/g mould; After solid state fermentation, 2.75 × 10 are contained in fermentation material8Cfu/g yeast, 1.48 × 107Cfu/g mould;I.e.:Before solid state fermentation Afterwards, Yeast proliferation is 6.04 times before fermentation, and mould abatement is 28.63% before fermentation.
Before solid state fermentation, cellulase activity is 46.8U/g;After solid state fermentation, cellulase activity is 78.5U/g, is increased Add as 1.68 times before fermentation.
The chicken health-care feed without vinasse local flavor, has vinasse local flavor before solid state fermentation after solid state fermentation.
Feeding experiment:
Sanhuang chicken (35 age in days) 40 is selected, 2 groups are bisected at random, test group feeds the chicken guarantor that the present embodiment is prepared Strong feed, the chicken basal feed described in control group fed the present embodiment, feeding experiment, 70 ages in days experiment knot since 35 ages in days Beam.
Concrete outcome see the table below:
Group Starting weight (kg) It is heavy (kg) eventually Daily gain (g) Daily ingestion amount (g) Feed-weight ratio
Test group 415±7.7 821±8.7* 11.6±0.6* 37.9 3.27
Control group 412±6.9 802±9.5 11.1±0.9 37.2 3.35
*:With control group significant difference, p<0.05
The above results show that test group brings up again high 2.37% (p eventually<0.05), daily gain improves 4.50%, and daily ingestion amount is carried High by 1.88%, feed-weight ratio improves 2.39%.
Compared with Example 1, the chicken health-care feed prepared by the comparative example has ferment local-flavor, and yeast and mould are increased Grow and cut down, increased after cellulase activity is fermented, but substantially less than embodiment 1, culture efficiency has compared with the control Clear superiority, but be far inferior to embodiment 1, illustrate linden black fungus bacterial rod for cellulase activity after feed fermentation raising extremely Close important, and feeding effect is had a significant impact.
Comparative example 8
The comparative example lacks linden black fungus bacterial rod compared with Example 1, in feed ingredient, and instead linden Lenlinus edodes Rod, remaining component and fermentation process all same.
The preparation method of one breeder health-care feed, is comprised the following steps that:
(1) linden black fungus bacterial rod is free of, and instead linden lentinus edodes strain stick 50kg, other feedstuffs and component are with real Apply example 1;
(2) the indoor water mire that mixture is placed in after running water is rinsed well, carries out nature solid state fermentation, fermented Journey be the same as Example 1;
(3) pelletization be the same as Example 1.
Before and after measure solid state fermentation, the quantity of yeast and mould, cellulase activity in fermentation material;And subjective appreciation ferments Front and rear feed quality.
As a result it is as follows:
Before solid state fermentation, treat to contain 5.58 × 10 in fermentation raw material7Cfu/g yeast, 7.63 × 107Cfu/g mould; After solid state fermentation, 3.96 × 10 are contained in fermentation material8Cfu/g yeast, 2.14 × 107Cfu/g mould;I.e.:Before solid state fermentation Afterwards, Yeast proliferation is 7.10 times before fermentation, and mould abatement is 28.05% before fermentation.
Before solid state fermentation, cellulase activity is 53.3U/g;After solid state fermentation, cellulase activity is 149.8U/g, is increased Add as 2.81 times before fermentation.
The chicken health-care feed without vinasse local flavor, has vinasse local flavor before solid state fermentation after solid state fermentation.
Feeding experiment:
Sanhuang chicken (35 age in days) 40 is selected, 2 groups are bisected at random, test group feeds the chicken guarantor that the present embodiment is prepared Strong feed, the chicken basal feed described in control group fed the present embodiment, feeding experiment, 70 ages in days experiment knot since 35 ages in days Beam.
Concrete outcome see the table below:
Group Starting weight (kg) It is heavy (kg) eventually Daily gain (g) Daily ingestion amount (g) Feed-weight ratio
Test group 407±8.8 814±10.2* 11.6±0.9 37.8 3.26
Control group 404±5.8 796±11.6 11.2±1.0 37.5 3.35
*:With control group significant difference, p<0.05
The above results show that test group brings up again high 2.26% (p eventually<0.05), daily gain improves 3.57%, and daily ingestion amount is carried High by 0.80%, feed-weight ratio improves 2.69%.
Compared with Example 1, the chicken health-care feed prepared by the comparative example has ferment local-flavor, and yeast and mould are increased Grow and cut down, rised appreciably after cellulase activity is fermented, but substantially less than embodiment 1, culture efficiency is compared with the control Have a clear superiority, be far inferior to embodiment 1, although illustrating that lentinus edodes strain stick participates in fermentation and can also improve the work of feed cellulase Power, but effect is not so good as linden black fungus bacterial rod.

Claims (9)

1. the preparation method of a breeder health-care feed, it is characterised in that comprise the following steps:
(1) Radix Astragali, radix glycyrrhizae, dried orange peel are well mixed with chicken basal feed, then add lees of yellow wine and linden black fungus thereto Bacterium rod, obtains mixture;
(2) mixture is carried out after solid state fermentation, fermentation ends, size-reduced, granulation obtains chicken health-care feed.
It is the Radix Astragali, sweet 2. preparation method as claimed in claim 1, it is characterised in that in terms of 1000kg chicken basal feed Grass, the addition of dried orange peel are followed successively by:15~20kg, 15~20kg, 15~20kg.
It is the Radix Astragali, sweet 3. preparation method as claimed in claim 1, it is characterised in that in terms of 1000kg chicken basal feed Grass, the addition of dried orange peel are followed successively by:17~18kg, 17~18kg, 17~18kg.
4. preparation method as claimed in claim 2 or claim 3, it is characterised in that the component of the chicken basal feed is:
5. preparation method as claimed in claim 4, it is characterised in that in step (1), the addition of the lees of yellow wine is 50 Density >=10 of yeast in~55kg, lees of yellow wine9cfu/g;The addition of the linden black fungus bacterial rod is 50~55kg.
6. preparation method as claimed in claim 1, it is characterised in that in step (2), the temperature of the solid state fermentation for 24~ 32 DEG C, the time is 45~50h, and every 12h stirrings once.
7. preparation method as claimed in claim 1, it is characterised in that in step (2), the laying depth of the mixture is 4 ~6cm.
8. preparation method as claimed in claim 1, it is characterised in that in step (2), is added before solid state fermentation into mixture Water, the addition of the water is the 20~30% of mixture quality.
9. preparation method as claimed in claim 1, it is characterised in that in step (2), the temperature of the granulation is 75~85 ℃。
CN201710193342.3A 2017-03-28 2017-03-28 The preparation method of one breeder health-care feed Pending CN106983033A (en)

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CN111418439A (en) * 2020-03-17 2020-07-17 常熟理工学院 Method for preparing needle mushroom sticks from curcuma aromatica dregs and broiler feed
CN111418440A (en) * 2020-03-17 2020-07-17 常熟理工学院 Method for preparing pleurotus eryngii fungus bags from radix adenophorae dregs and broiler feed
CN113229404A (en) * 2021-06-17 2021-08-10 吉林农业科技学院 Compound traditional Chinese medicine preparation special for promoting growth of poultry by composite probiotic fermentation and preparation method thereof

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CN105961824A (en) * 2016-04-20 2016-09-28 义乌市锦钰信息科技有限公司 Feed additive product for chickens

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CN104585478A (en) * 2014-12-17 2015-05-06 隋进清 Black fungus chaff feed processing and manufacturing technology using straw
CN104824389A (en) * 2015-04-13 2015-08-12 合肥市福来多食品有限公司 Digestible feed for laying hen and preparation method thereof
CN105432968A (en) * 2016-01-09 2016-03-30 黄庆梅 Heat stress-resistant chicken feed additive
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CN107616153A (en) * 2017-09-29 2018-01-23 湖北龙王恨渔具集团有限公司 A kind of method of bait nest material and its disinfectant fresh-keeping using pasteurization
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CN111418440A (en) * 2020-03-17 2020-07-17 常熟理工学院 Method for preparing pleurotus eryngii fungus bags from radix adenophorae dregs and broiler feed
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CN113229404A (en) * 2021-06-17 2021-08-10 吉林农业科技学院 Compound traditional Chinese medicine preparation special for promoting growth of poultry by composite probiotic fermentation and preparation method thereof

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Application publication date: 20170728