CN106982859B - A kind of compounded pesticides of Lecanicillium lecanii and Osthole - Google Patents
A kind of compounded pesticides of Lecanicillium lecanii and Osthole Download PDFInfo
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- CN106982859B CN106982859B CN201710282995.9A CN201710282995A CN106982859B CN 106982859 B CN106982859 B CN 106982859B CN 201710282995 A CN201710282995 A CN 201710282995A CN 106982859 B CN106982859 B CN 106982859B
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- osthole
- lecanicillium lecanii
- compounded pesticides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
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Abstract
The invention discloses the compounded pesticides and its application of a kind of Lecanicillium lecanii and Osthole.The compounded pesticides are made up of host and assistant agent, and host is 10~40% by mass percentage, and assistant agent is 60~90%；The host is the mixture of Lecanicillium lecanii and Osthole, Lecanicillium lecanii: Osthole mass ratio is 10: 1.The compounded pesticides of the present invention can be processed into wettable powder, and the compounded pesticides have good prevention effect to insect aleyrodid and aphid.
Description
Technical field
The invention belongs to technical field of pesticide, it is related to the compound insecticidal of a kind of environment amenable Lecanicillium lecanii and Osthole
Agent.
Background technology
Lecanicillium lecanii (Lecanicillium lecanii) is a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals,
With the brand-new mechanism of action different from chemical insecticide, environment amenable feature, preventing and treating can be used as some to chemistry
The replacement protective agents for the insect that insecticide develops immunity to drugs, utilization Lecanicillium lecanii have important economic implications.Lecanicillium lecanii
Geographical distribution is extensive, can be used for preventing and treating a variety of important pests such as aleyrodid, aphid, scale insect, wherein most successful control and application
It is the aleyrodid and aphid in warmhouse booth, has a good application prospect.Scientific investigations showed that different geographic populations or not homophyletic
There is larger difference to the virulence of insect in the Lecanicillium lecanii of system, even if the Lecanicillium lecanii of same strain is to the virulence of variety classes insect
Also differ, therefore high virulence is often screened for different types of insect during application fungi carries out biological control
Bacterial strain.Lecanicillium lecanii is as other entomogenous fungis, and there is also knocking down, the insect time is long, desinsection speed is slow, affected by environment larger
Etc. drawback, especially when insect density is larger, it is impossible to force down insect population in time, retrieve a loss.
Osthole is the active ingredient that extraction obtains from samphire frutus cnidii (Cnidium monnieri), its
Chemical name is 7- methoxyl groups -8- (3 '-methyl -2 '-cyclobutenyl) -1- dihydrobenzopyrans ketone -2, belongs to Coumarins chemical combination
Thing, based on action of contace poison, there is preferably preventing and treating to imitate to insects such as brassicaceous vegetable aleyrodid, aphid and tea tree tea geometrids
Fruit.Osthole has the advantages that environmentally friendly, micro- poison in crop pests preventing and treating, is not easy to make insect develop immunity to drugs,
It is to produce the pesticide species that non-polluted farm product is preferentially selected.
Lecanicillium lecanii, using the dosage that can substantially reduce Osthole in practice in agricultural production, delays with fructus cnidii complex
For insect to process caused by Osthole resistance, the Osthole of sub-doses mixes with Lecanicillium lecanii can also effectively improve fungi desinsection
The quick-acting of agent, while protect ecological environment.Therefore, the optimal compound proportion of Lecanicillium lecanii and Osthole is screened, will be had
It is significant.At present both at home and abroad there is not yet the relevant report of the insecticide of Lecanicillium lecanii and fructus cnidii complex.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of environment amenable Lecanicillium lecanii and frutus cnidii
The compounded pesticides of element.
Another object of the present invention is to provide the application of the compounded pesticides.
Above-mentioned purpose of the present invention is achieved through the following technical solutions：
The invention provides a kind of Lecanicillium lecanii and the compounded pesticides of Osthole, it is made up of host and assistant agent, by quality
Percentages host is 10~40%, and assistant agent is 60~90%；The host is the mixture of Lecanicillium lecanii and Osthole, lecanium
Bacterium: Osthole mass ratio is 50: 1~10.
Described lecanium bacteria strain GLLE9606, is located away from the aleyrodid polypide infected in Langfang in Hebei Province area, and bacterial strain is protected
Tibetan number is CGMCC No.8453, and on November 11st, 2013 is in China Committee for Culture Collection of Microorganisms's common micro-organisms
Heart preservation.Lecanium bacteria strain GLLE9606 is screened from numerous similar bacterial strains, dedicated for preventing and treating aleyrodid class pest
Lecanicillium lecanii sociales bacterial strain, have the characteristics that virulence is strong, desinsection speed is fast, environmentally friendly, and obtain the hair of country's mandate
Bright patent (ZL201310560723.2).
In order to be better achieved the present invention, the mass ratio of the Lecanicillium lecanii and Osthole is preferably 50: 1.5~6.
As a preferred embodiment, the Lecanicillium lecanii is Lecanicillium lecanii conidial powder.
The assistant agent is stabilizer, auxiliary agent and filler, and by mass percentage, stabilizer accounts for compounded pesticides 1~10%,
Auxiliary agent accounts for compounded pesticides 1~20%, and filler accounts for compounded pesticides 30~88%.
The stabilizer is glucose (powder) and the mixture of starch one or two；The auxiliary agent is washing powder, ten
One or both of dialkyl sulfonates, sodium lignin sulfonate, wetting agent BENOL790A, dispersant B D55 thing mixed above；
The filler is white carbon, talcum powder, clay, diatomite, kaolin or clay.
Compounded pesticides of the present invention can be processed into wettable powder.
Present invention simultaneously provides application of the compounded pesticides in pest control aleyrodid and aphid.
The present invention compared with prior art, has the following advantages that and beneficial effect：
The bacterial strain that the present invention uses is to separate to obtain on the aleyrodid polypide infected from Langfang in Hebei Province area, is China domestic
Bacterial strain, not from foreign countries introduce, local natural environment can be well adapted for.The present invention has adapted to the need of organic foodstuff production
Ask, environmentally safe, noresidue, be advantageous to delay pest resistance to insecticide to produce.Compounded pesticides of the present invention have significant increase
Effect acts on, and is better than the prevention effect of single kind of medicament to the prevention effect of insect, has good preventing and treating to insect aleyrodid and aphid
Effect.
Embodiment
Inventor has found the mixture of Lecanicillium lecanii and Osthole to insect aleyrodid and aphid by substantial amounts of Seiving Tests
Worm has synergistic function.In order to be better understood from the essence of invention, the technology contents of the present invention are made with reference to example
Detailed description, but present disclosure is not so limited, it is impossible to therefore and it is interpreted as the limitation to the scope of the claims of the present invention.
Percentage described in following examples is mass percent.Described lecanium bacteria strain is GLLE9606, bacterial strain
Preserving number is CGMCC No.8453, and on November 11st, 2013 is in China Committee for Culture Collection of Microorganisms's common micro-organisms
Center preservation.
Embodiment 1：Lecanicillium lecanii and the screening of Osthole mixed ratio
Subjects are the age nymph of Bemisia tabaci (Bemisia tabaci) two, test medicine be Lecanicillium lecanii, Osthole and
The mixture preparation of Lecanicillium lecanii and Osthole.Lecanicillium lecanii be 10,000,000,000 spores/gram pure cryptogam, Osthole is content 98%
Active compound.
Test method is with reference to People's Republic of China's agricultural industry criteria《Farm-chemical indoor determination test rule》Desinsection
Agent NY/T 1154.9-2008, experiment use spraying treatment method.By the Lecanicillium lecanii spore suspension of series concentration, Osthole and
The mixture preparation of Lecanicillium lecanii and Osthole is sprayed on the cucumber leaves with Bemisia tabaci two ages nymph with miniaturised nebuliser, control
For sterilized water, naturally dry.Often processing is 3 leaves, per 100 nymphs of leaf, 3 repetitions.Place a plant into transparent freshness protection package
In, place into 25 ± 1 DEG C of illumination box (L: D=16: 8), the dead borer population of the 7th day record.It is soft with DPS data processings
Part carries out statistical analysis, calculates the LC of each medicament50, the activity of reagent agent is evaluated with this.
The co-toxicity coefficient (CTC values) of mixture is calculated by following equation：
ATI=S/M × 100
In formula：ATI is that mixture surveys toxicity index；S is the LC of standard pesticide agent50, unit mg/L；M is mixture
LC50, unit mg/L.
TTI=TIA × PA+TIB × PB
In formula：TTI is mixture theoretical toxicity index；TIA is A medicament toxicity index；PA is percentage of the A medicaments in mixture
Content, unit are percentage；TIB is B medicament toxicity index；PB is percentage composition of the B medicaments in mixture, and unit is percentage
Rate.
CTC=ATI/TTI × 100
In formula：CTC is co-toxicity coefficient；ATI is that mixture surveys toxicity index；TTI is mixture theoretical toxicity index.
CTC >=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜ CTC ＜ 120, which show as being added, to be made
With.
Table 1：Each single dose and its mixture are to the Bemisia tabaci two ages nymph toxicity test result of 7 days
| Medicament title | LC50(mg/L) | ATI | TTI | Co-toxicity coefficient CTC |
| Lecanicillium lecanii (A) | 9.50 | 100.00 | - | - |
| Osthole (B) | 8.73 | 108.82 | - | - |
| A: B=20: 1 | 6.16 | 154.22 | 100.42 | 153.58 |
| A: B=10: 1 | 4.64 | 204.74 | 100.80 | 203.11 |
| A: B=1: 1 | 7.03 | 135.14 | 104.41 | 129.43 |
| A: B=1: 10 | 7.14 | 133.05 | 108.02 | 123.18 |
| A: B=1: 20 | 7.21 | 131.76 | 108.40 | 121.55 |
As a result as shown in table 1, Toxicity Determination result shows, Lecanicillium lecanii and Osthole are to Bemisia tabaci two ages nymph 7
It LC50Respectively 9.50mg/L and 8.73mg/L.Lecanicillium lecanii: (Lecanicillium lecanii when Osthole mass ratio is 20: 1 to 1: 20
When conidium number and Osthole grams ratio are 2000~10,000,000,000: 1~20), co-toxicity coefficient (CTC) is all higher than 120, says
Bright each proportioning is respectively provided with synergistic effect, especially Lecanicillium lecanii: (Lecanicillium lecanii conidium when Osthole mass ratio is 10: 1
When number and Osthole grams ratio are 100,000,000,000: 1), co-toxicity coefficient (CTC) is more than 200, and synergistic effect is notable.
Embodiment 2：20% Lecanicillium lecanii Osthole wettable powder and its preventive effect to Bemisia tabaci on greenhouse cucumber
The pure cryptogam of Lecanicillium lecanii (10,000,000,000 spores/gram) 18.18%, Osthole (98% active compound level) 1.82%, starch 3%,
Dispersant WLND100 3%, dodecyl sodium sulfate 3%, white carbon are mended to 100%, and 20% Lecanicillium lecanii Osthole is made can
WP.Mixture preparation adds 3000 times of dilutions of water, and spraying prevents and treats Bemisia tabaci (Bemisia tabaci) on greenhouse cucumber, works as temperature
Room indoor temperature is 26 DEG C, and during relative humidity 85%, the insect cumulative mortality of 7 days reaches 96.8%.
Embodiment 3：10% Lecanicillium lecanii Osthole wettable powder and its preventive effect to cotten aphid on greenhouse cucumber
The pure cryptogam of Lecanicillium lecanii (10,000,000,000 spores/gram) 9.09%, Osthole (98% active compound level) 0.91%, starch 3%,
Dispersant WLND100 3%, dodecyl sodium sulfate 3%, white carbon are mended to 100%, and 10% Lecanicillium lecanii Osthole is made can
WP.Mixture preparation adds 1500 times of dilutions of water, and spraying prevents and treats cotten aphid (Aphis gossypii) on greenhouse cucumber, works as greenhouse
Indoor temperature is 26 DEG C, and during relative humidity 85%, the insect cumulative mortality of 7 days reaches 98.5%.
Claims (2)
- A kind of 1. compounded pesticides of Lecanicillium lecanii and Osthole, it is characterised in that the compounded pesticides are wettable powder, It is made up of host and assistant agent, host is 10~40% by mass percentage, and assistant agent is 60~90%；The host is the mixture of Lecanicillium lecanii and Osthole, and Lecanicillium lecanii: Osthole mass ratio is 10: 1, Lecanicillium lecanii is 10000000000 spores/gram pure cryptogam, Osthole be content 98% active compound；Described Lecanicillium lecanii (Lecanicillium lecanii) is bacterial strain GLLE9606, and bacterial strain preserving number is CGMCC No.8453, on November 11st, 2013 is in China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation.
- 2. application of the compounded pesticides described in claim 1 in pest control aleyrodid and aphid.
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| CN201710282995.9A CN106982859B (en) | 2017-04-26 | 2017-04-26 | A kind of compounded pesticides of Lecanicillium lecanii and Osthole |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195047A (en) * | 2013-11-13 | 2014-12-10 | 谢明 | Lecanicillium lecanii and application of Lecanicillium lecanii to control of greenhouse pests |
| CN104542079A (en) * | 2013-10-16 | 2015-04-29 | 中国农业科学院蔬菜花卉研究所 | Method for processing drug-containing slow-release material and preventing and treating vegetable diseases and insect pests |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2003335612A (en) * | 2002-05-17 | 2003-11-25 | Japan Science & Technology Corp | Microorganism-pesticide fixable onto surface of plant body |
| JP4644478B2 (en) * | 2004-12-13 | 2011-03-02 | クミアイ化学工業株式会社 | Verticillium lecani YK-920 strain and pest and plant disease control agent using the same |
| CN104488972A (en) * | 2014-11-27 | 2015-04-08 | 青岛崂乡茶制品有限公司 | Biological pesticide with wide spectrum and high efficiency |
| CN106538611A (en) * | 2016-10-20 | 2017-03-29 | 中国农业科学院棉花研究所 | From bactericide its preparation method and the application of frutus cnidii |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542079A (en) * | 2013-10-16 | 2015-04-29 | 中国农业科学院蔬菜花卉研究所 | Method for processing drug-containing slow-release material and preventing and treating vegetable diseases and insect pests |
| CN104195047A (en) * | 2013-11-13 | 2014-12-10 | 谢明 | Lecanicillium lecanii and application of Lecanicillium lecanii to control of greenhouse pests |
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