CN106975103A - A kind of spider silk fibroin tissue renovation material containing kuh-seng - Google Patents

A kind of spider silk fibroin tissue renovation material containing kuh-seng Download PDF

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Publication number
CN106975103A
CN106975103A CN201710206357.9A CN201710206357A CN106975103A CN 106975103 A CN106975103 A CN 106975103A CN 201710206357 A CN201710206357 A CN 201710206357A CN 106975103 A CN106975103 A CN 106975103A
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silk fibroin
spider silk
solution
parts
seng
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吴长应
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Wuhu Yangzhan New Material Technology Service Co Ltd
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Wuhu Yangzhan New Material Technology Service Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • D—TEXTILES; PAPER
    • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
    • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
    • D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
    • D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
    • D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
    • D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/41—Anti-inflammatory agents, e.g. NSAIDs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/412—Tissue-regenerating or healing or proliferative agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/34—Materials or treatment for tissue regeneration for soft tissue reconstruction

Abstract

A kind of spider silk fibroin tissue renovation material containing kuh-seng, for the membranoid substance comprising spider silk fibroin, internal average cell density is 1 2000/mm2, average pore size is 1 300 μm, it is characterised in that described membranoid substance includes the raw material of following parts by weight：70 90 parts of spidroin solution, 56 parts of spider silk fibroin nanofiber solution, 12 parts of Radix Sophorae Flavescentis extractive liquid, 12 parts of celecoxib, 12 parts of essential oil.The present invention relates to biomaterial and spider silk fibroin field.The biomaterial of the big tissue and complex organ of repairing damage must have the ability for promoting vascularization big tissue and complex organ is had biological function.But it is generally in the prior art simple fibroin albumen repair materials, is extremely difficult to preferable rush vascularization and tissue repair function, and tissue renovation material produced by the present invention has the effect of more preferable tissue repair.

Description

A kind of spider silk fibroin tissue renovation material containing kuh-seng
Technical field
The present invention relates to biomaterial and spider silk fibroin field, more particularly to a kind of spider silk fibroin tissue containing kuh-seng Repair materials.
Background technology
Tissue engineering technique is a new tool for being used to treat tissue and organ defect, by biological support, cell, growth The elements such as the factor are constituted.Wherein, the physical support that biological support is formed as cell growth, propagation and new organization, in tissue Played an important role in engineering.Organizational project is intended to the 26S Proteasome Structure and Function integrality of restoration and reconstruction damaged tissues or organ, and shows The present can't solve the reparation of complicated tissue organ.Big tissue, complex organ can pass through small point unlike simple lamellar tissue Son diffusion obtains nutriment and oxygen, and they need that by huge vascular system nutriment and oxygen could be obtained.Repair The big tissue and the biomaterial of complex organ damaged again must have the ability for promoting vascularization just to make big tissue and complicated Organ has biological function.
Fibroin albumen has excellent biocompatibility, degradability, improvability and mechanical stretching performance etc..Fibroin egg Contain hydroxyl, amino, carboxyl isoreactivity group in white, hydrogen bond, electrostatic interaction, chelating can be passed through with other high molecular polymers Effect etc. is combined, the superior compound bio timbering material of forming properties.But it is generally simple fibroin albumen material in the prior art Material, is extremely difficult to preferable rush vascularization and tissue repair function.
The content of the invention
For problems of the prior art, the invention provides a kind of spider silk fibroin tissue repair material containing kuh-seng Material, compared with the prior art in simple fibroin material there is more preferable tissue repair function.
In order to solve the above problems, the present invention uses following technical scheme：
A kind of spider silk fibroin tissue renovation material containing kuh-seng, for the membranoid substance comprising spider silk fibroin, inside is average Hole density is 1-2000/mm2, average pore size is 1-300 μm, and described membranoid substance includes the raw material of following parts by weight：Spider 70-90 parts of spider's thread protein solution, 5-6 parts of spider silk fibroin nanofiber solution, 1-2 parts of Radix Sophorae Flavescentis extractive liquid, celecoxib 1-2 Part, 1-2 parts of essential oil.
It is preferred that, described Radix Sophorae Flavescentis extractive liquid is prepared using following methods：Kuh-seng 25g is weighed, coarse powder is ground into, it is added The water that 10 times of weight, decocts under the conditions of 90-100 DEG C and extracts 1-1.5h, suction filtration obtains filtrate 1；6 times of filter residues are added into filter residue again The distilled water of weight, decocts under the conditions of 70-80 DEG C and extracts 1-1.5h, suction filtration obtains filtrate 2；Filtrate 1, filtrate is 2-in-1 simultaneously, in temperature Degree is 40-50 DEG C, vacuum is 4 times that kuh-seng weight is concentrated under reduced pressure under the conditions of 0.05-0.08Pa, and filtering produces kuh-seng extraction Liquid.
It is preferred that, described spider silk fibroin nanofiber solution is prepared using following methods：Take spidroin solution It is placed in 60 DEG C of insulating boxs and is concentrated into the half of original volume, then is transferred in fume hood and is concentrated into mass fraction and obtains dense for 20-30% Contracting liquid, it is 0.5% concentrate to be put into after 4 DEG C of refrigerator storage 2d concentrate is diluted with water into mass fraction, full and uniform to stir It is put into insulating box and is incubated to the spider silk fibroin nanofiber solution for forming translucent gels shape after mixing.
It is preferred that, the length of spider silk fibroin nanofiber is 1 μm in described spider silk fibroin nanofiber solution, directly Footpath is 10-20nm.
It is preferred that, described spidroin solution is prepared using following methods：2L deionized waters are heated to boiling, plus Enter 4.24g sodium carbonate and 5g spider silks, boil 30min, after being cleaned up, drying with deionized water, with 9.3mol/L LiBr Solution dissolves；Solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated Twice, spidroin solution is obtained.
It is preferred that, described spidroin solution is spider silk silk fibroin protein solution.
It is preferred that, described membranoid substance is prepared using following methods：By spidroin solution and spider silk fibroin nanometer Fiber solution is mixed, and adds the well mixed obtained spinning solution of Radix Sophorae Flavescentis extractive liquid, celecoxib, essential oil, and spinning solution uses electrostatic Three-D non-woven net-structured film is made in spinning technique.
It is further preferred that described electrostatic spinning process is：Voltage 12-18KV, spinning height 5-10cm, winding speed 100-300rpm, spinning solution flow 2-5ml/h, 50-150 DEG C of heating-up temperature.
It is preferred that, fibre diameter is 50nm-20um in described three-D non-woven net-structured film, and interfiber pore is l- 500um, film thickness is 50nm-20mm.
The beneficial effects of the present invention are：
1) tissue renovation material of the invention is relative to simple silk fibroin material, and the soft tissue for low modulus is repaiied Multiple material, vascularization and tissue repair function are promoted with more preferable.
2) three-dimensional porous material for organizational project must have higher porosity, with interconnected three-dimensional knot Structure, propagation, migration in order to cell, enough nutriments are provided for the growth of cell.Add after nanofiber, improve There are nanofibrous structures on the pore-creating character support hole wall of support, the specific surface area of support is considerably increased.
3) medicinal herb componentses Radix Sophorae Flavescentis extractive liquid, and celecoxib, essential oil are added, materials microstructure reparation is further increased Ability.
4) kuh-seng can treat swelling of vulva pruritus vulvae, and eczema, wet sore, pruitus, mange leprosy controls trichomonas vaginitis outside.
Embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of spider silk fibroin tissue renovation material containing kuh-seng, for the membranoid substance comprising spider silk fibroin, inside is average Hole density is 1-2000/mm2, average pore size is 1-300 μm, it is characterised in that described membranoid substance includes following parts by weight Several raw materials：70 parts of spidroin solution, 5 parts of spider silk fibroin nanofiber solution, 1 part of Radix Sophorae Flavescentis extractive liquid, celecoxib 1 Part, 1 part of essential oil.
Described Radix Sophorae Flavescentis extractive liquid is prepared using following methods：Kuh-seng 25g is weighed, coarse powder is ground into, its weight 10 is added Water again, decocts under the conditions of 90-100 DEG C and extracts 1h, suction filtration obtains filtrate 1；The distillation of 6 times of filter residue weight is added into filter residue again Water, decocts under the conditions of 70-80 DEG C and extracts 1h, suction filtration obtains filtrate 2；Filtrate 1, filtrate is 2-in-1 simultaneously, it is 40-50 DEG C, very in temperature Reciprocal of duty cycle is is concentrated under reduced pressure into 4 times of kuh-seng weight under the conditions of 0.05-0.08Pa, filtering produces Radix Sophorae Flavescentis extractive liquid.
Described spider silk fibroin nanofiber solution is prepared using following methods：Spidroin solution is taken to be placed in 60 DEG C The half of original volume is concentrated into insulating box, then be transferred in fume hood be concentrated into mass fraction for 20% concentrate, will concentrate It is 0.5% that liquid, which is put into after 4 DEG C of refrigerator storage 2d concentrate being diluted with water mass fraction, and constant temperature is put into after full and uniform stirring It is incubated in case to the spider silk fibroin nanofiber solution for forming translucent gels shape.
The length of spider silk fibroin nanofiber is 1 μm, a diameter of 10- in described spider silk fibroin nanofiber solution 20nm。
Described spidroin solution is prepared using following methods：2L deionized waters are heated to boiling, 4.24g is added Sodium carbonate and 5g spider silks, boil 30min, molten with 9.3mol/L LiBr solution after being cleaned up, drying with deionized water Solution；Solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice, Obtain spidroin solution.
Described spidroin solution is spider silk silk fibroin protein solution.
Described membranoid substance is prepared using following methods：By spidroin solution and spider silk fibroin nanofiber solution Mixing, and the well mixed obtained spinning solution of Radix Sophorae Flavescentis extractive liquid, celecoxib, essential oil is added, spinning solution uses electrostatic spinning process Three-D non-woven net-structured film is made.
Described electrostatic spinning process is：Voltage 12-18KV, spinning height 5-10cm, winding speed 100-300rpm, spin Silk flow quantity 2-5ml/h, 50-150 DEG C of heating-up temperature.
Fibre diameter is 50nm-20um in described three-D non-woven net-structured film, and interfiber pore is l-500um, film Thickness is 50nm-20mm.
Embodiment 2
A kind of spider silk fibroin tissue renovation material containing kuh-seng, for the membranoid substance comprising spider silk fibroin, inside is average Hole density is 1-2000/mm2, average pore size is 1-300 μm, it is characterised in that described membranoid substance includes following parts by weight Several raw materials：90 parts of spidroin solution, 6 parts of spider silk fibroin nanofiber solution, 2 parts of Radix Sophorae Flavescentis extractive liquid, celecoxib 2 Part, 2 parts of essential oil.
Described Radix Sophorae Flavescentis extractive liquid is prepared using following methods：Kuh-seng 25g is weighed, coarse powder is ground into, its weight 10 is added Water again, decocts under the conditions of 90-100 DEG C and extracts 1.5h, suction filtration obtains filtrate 1；The steaming of 6 times of filter residue weight is added into filter residue again Distilled water, decocts under the conditions of 70-80 DEG C and extracts 1.5h, suction filtration obtains filtrate 2；Filtrate 1, filtrate is 2-in-1 simultaneously, it is 40-50 in temperature DEG C, vacuum be to be concentrated under reduced pressure into 4 times of kuh-seng weight under the conditions of 0.05-0.08Pa, filtering produces Radix Sophorae Flavescentis extractive liquid.
Described spider silk fibroin nanofiber solution is prepared using following methods：Spidroin solution is taken to be placed in 60 DEG C The half of original volume is concentrated into insulating box, then be transferred in fume hood be concentrated into mass fraction for 30% concentrate, will concentrate It is 0.5% that liquid, which is put into after 4 DEG C of refrigerator storage 2d concentrate being diluted with water mass fraction, and constant temperature is put into after full and uniform stirring It is incubated in case to the spider silk fibroin nanofiber solution for forming translucent gels shape.
The length of spider silk fibroin nanofiber is 1 μm, a diameter of 10- in described spider silk fibroin nanofiber solution 20nm。
Described spidroin solution is prepared using following methods：2L deionized waters are heated to boiling, 4.24g is added Sodium carbonate and 5g spider silks, boil 30min, molten with 9.3mol/L LiBr solution after being cleaned up, drying with deionized water Solution；Solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice, Obtain spidroin solution.
Described spidroin solution is spider silk silk fibroin protein solution.
Described membranoid substance is prepared using following methods：By spidroin solution and spider silk fibroin nanofiber solution Mixing, and the well mixed obtained spinning solution of Radix Sophorae Flavescentis extractive liquid, celecoxib, essential oil is added, spinning solution uses electrostatic spinning process Three-D non-woven net-structured film is made.
Described electrostatic spinning process is：Voltage 12-18KV, spinning height 5-10cm, winding speed 100-300rpm, spin Silk flow quantity 2-5ml/h, 50-150 DEG C of heating-up temperature.
Fibre diameter is 50nm-20um in described three-D non-woven net-structured film, and interfiber pore is l-500um, film Thickness is 50nm-20mm.
By tissue renovation material (SS) made from tissue renovation material (S) made from embodiment 1, embodiment 2, and by pure silkworm The performance for the tissue renovation material (SSS) that silk silk fibroin albumen is made is contrasted, using with identical degree of tissue damage Small white mouse tested, material S, SS and SSS are spread on to the injury site of small white mouse respectively, daily change twice, continuously make With 10 days, using observation point system, small white mouse wounded tissue recovery extent is given a mark by 1-5 points respectively, items use effect Really optimal is 5 points, and the experimental data drawn is shown in Table 1.
The resultant effect of table 1 is investigated
Material S SS SSS
Tissue repair degree 4.8 4.9 2.9
Antiphlogistic effects 4.8 4.9 2.6
By experimental result it can be seen that：Fibroin is substituted using spider silk fibroin, and added into spider silk fibroin albumen Plus spider silk fibroin nanofiber, kuh-seng, celecoxib and essential oil, can effectively be lifted fibroin albumen tissue repairing ability and Antiphlogistic effects.

Claims (8)

1. a kind of spider silk fibroin tissue renovation material containing kuh-seng, for the membranoid substance comprising spider silk fibroin, the average hole in inside Density is 1-2000/mm2, average pore size is 1-300 μm, it is characterised in that described membranoid substance includes following parts by weight Raw material：70-90 parts of spidroin solution, 5-6 parts of spider silk fibroin nanofiber solution, 1-2 parts of Radix Sophorae Flavescentis extractive liquid, plug come Cloth 1-2 parts of former times, 1-2 parts of essential oil；
Described Radix Sophorae Flavescentis extractive liquid is prepared using following methods：Kuh-seng 25g is weighed, coarse powder is ground into, 10 times of its weight is added Water, decocts under the conditions of 90-100 DEG C and extracts 1-1.5h, suction filtration obtains filtrate 1；The distillation of 6 times of filter residue weight is added into filter residue again Water, decocts under the conditions of 70-80 DEG C and extracts 1-1.5h, suction filtration obtains filtrate 2；Filtrate 1, filtrate is 2-in-1 simultaneously, it is 40-50 in temperature DEG C, vacuum be to be concentrated under reduced pressure into 4 times of kuh-seng weight under the conditions of 0.05-0.08Pa, filtering produces Radix Sophorae Flavescentis extractive liquid.
2. a kind of spider silk fibroin tissue renovation material containing kuh-seng according to claim 1, it is characterised in that described Spider silk fibroin nanofiber solution is prepared using following methods：Take spidroin solution to be placed in 60 DEG C of insulating boxs to be concentrated into The half of original volume, then be transferred in fume hood and be concentrated into mass fraction and obtain concentrate for 20-30%, concentrate is put into 4 DEG C of ice It is 0.5% that concentrate is diluted with water into mass fraction after case storage 2d, is put into insulating box and is incubated extremely after full and uniform stirring Form the spider silk fibroin nanofiber solution of translucent gels shape.
3. a kind of spider silk fibroin tissue renovation material containing kuh-seng according to claim 2, it is characterised in that：Described The length of spider silk fibroin nanofiber is 1 μm, a diameter of 10-20nm in spider silk fibroin nanofiber solution.
4. a kind of spider silk fibroin tissue renovation material containing kuh-seng according to claim 1, it is characterised in that described Spidroin solution is prepared using following methods：2L deionized waters are heated to boiling, 4.24g sodium carbonate and 5g spiders is added Silk, boils 30min, after being cleaned up, dry with deionized water, with 9.3mol/L LiBr solution dissolving；Until completely dissolved Solution is loaded into bag filter, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice, obtains spider silk fibroin molten Liquid.
5. a kind of spider silk fibroin tissue renovation material containing kuh-seng according to claim 1, it is characterised in that：Described Spidroin solution is spider silk silk fibroin protein solution.
6. a kind of spider silk fibroin tissue renovation material containing kuh-seng according to claim 1, it is characterised in that described Membranoid substance is prepared using following methods：Spidroin solution is mixed with spider silk fibroin nanofiber solution, and adds hardship Join the well mixed obtained spinning solution of extract solution, celecoxib, essential oil, three-D non-woven net is made using electrostatic spinning process in spinning solution Shape structural membrane.
7. a kind of spider silk fibroin tissue renovation material containing kuh-seng according to claim 6, it is characterised in that described Electrostatic spinning process is：Voltage 12-18KV, spinning height 5-10cm, winding speed 100-300rpm, spinning solution flow 2-5ml/ H, 50-150 DEG C of heating-up temperature.
8. a kind of spider silk fibroin tissue renovation material containing kuh-seng according to claim 6, it is characterised in that：Described Fibre diameter is 50nm-20um in three-D non-woven net-structured film, and interfiber pore is 1-500um, and film thickness is 50nm- 20mm。
CN201710206357.9A 2017-03-31 2017-03-31 A kind of spider silk fibroin tissue renovation material containing kuh-seng Pending CN106975103A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110129997A (en) * 2019-06-05 2019-08-16 安徽迈德普斯医疗科技有限公司 A kind of non-woven fabrics and processing method of quick water suction networking partition

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Application publication date: 20170725