CN106823000A - A kind of fibroin tissue renovation material containing cicada slough - Google Patents
A kind of fibroin tissue renovation material containing cicada slough Download PDFInfo
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- CN106823000A CN106823000A CN201710206192.5A CN201710206192A CN106823000A CN 106823000 A CN106823000 A CN 106823000A CN 201710206192 A CN201710206192 A CN 201710206192A CN 106823000 A CN106823000 A CN 106823000A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/362—Skin, e.g. dermal papillae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
- A61L27/3679—Hollow organs, e.g. bladder, esophagus, urether, uterus, intestine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/21—Acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/22—Materials or treatment for tissue regeneration for reconstruction of hollow organs, e.g. bladder, esophagus, urether, uterus
Abstract
A kind of fibroin tissue renovation material containing cicada slough, is the membranoid substance comprising fibroin, and internal average cell density is 1 3000/mm2, average pore size is 1 500 μm, described raw material of the membranoid substance comprising following parts by weight:115 125 parts of fibroin solutions, 78 parts of fibroin nanofiber solution, 23 parts of cicada slough extract solution, 12 parts of Diclofenac, 12 parts of essential oil.The present invention relates to biomaterial and fibroin field.Big tissue and the biomaterial of complex organ must can just make to organize greatly with the ability for promoting vascularization and complex organ has biological function that reparation is damaged.But it is generally in the prior art simple fibroin albumen repair materials, is extremely difficult to preferable rush vascularization and tissue repair function, and the obtained tissue renovation material of the present invention has the effect of more preferable tissue repair.
Description
Technical field
The present invention relates to biomaterial and fibroin field, more particularly to a kind of fibroin tissue repair containing cicada slough
Material.
Background technology
Tissue engineering technique is a new tool for being used to treat tissue and organ defect, by biological support, cell, growth
The elements such as the factor are constituted.Wherein, the physical support that biological support is formed as cell growth, propagation and new organization, in tissue
Played an important role in engineering.Organizational project is intended to the 26S Proteasome Structure and Function integrality of restoration and reconstruction damaged tissues or organ, and shows
The present can't solve the reparation of complicated tissue organ.Big tissue, complex organ can be by small points unlike simple lamellar tissue
Son diffusion obtains nutriment and oxygen, and they need that by huge vascular system nutriment and oxygen could be obtained.Repair
The big tissue of multiple damage and the biomaterial of complex organ must can just make big tissue and complexity with the ability for promoting vascularization
Organ has biological function.
Fibroin albumen has excellent biocompatibility, degradability, improvability and mechanical stretching performance etc..Fibroin egg
Contain hydroxyl, amino, carboxyl isoreactivity group in white, hydrogen bond, electrostatic interaction, chelating can be passed through with other high molecular polymers
Effect etc. is combined, the superior compound bio timbering material of forming properties.But it is generally in the prior art simple fibroin albumen material
Material, is extremely difficult to preferable rush vascularization and tissue repair function.
The content of the invention
For problems of the prior art, the invention provides a kind of fibroin tissue repair material containing cicada slough
Material, compared with the prior art in simple fibroin material there is more preferable tissue repair function.
In order to solve the above problems, the present invention uses following technical scheme:
A kind of fibroin tissue renovation material containing cicada slough, is the membranoid substance comprising fibroin, internal average Kong Mi
It is 1-3000/mm to spend2, average pore size is 1-500 μm, described raw material of the membranoid substance comprising following parts by weight:Silk egg
White 115-125 parts of solution, 7-8 parts of fibroin nanofiber solution, 2-3 parts of cicada slough extract solution, Diclofenac 1-2 parts, essential oil
1-2 parts.
Preferably, described fibroin nanofiber solution is prepared using following methods:Fibroin solutions are taken to be placed in
The half of original volume is concentrated into 60 DEG C of insulating boxs, then is transferred in fume hood and is concentrated into mass fraction for 20-30% obtains concentrate,
It is 0.5% concentrate to be put into concentrate is diluted with water into mass fraction after 4 DEG C of refrigerator storage 2d, is put after full and uniform stirring
Enter and be incubated in insulating box to the fibroin nanofiber solution for forming translucent gels shape.
Preferably, the length of fibroin nanofiber is 1 μm in described fibroin nanofiber solution, a diameter of
10-20nm。
Preferably, described fibroin solutions are prepared using following methods:2L deionized waters are heated to boiling, is added
4.24g sodium carbonate and 5g silkworm raw silks, boil 30min, after being cleaned up with deionized water, drying, with the LiBr of 9.3mol/L
Solution dissolves;Solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated
Twice, fibroin solutions are obtained.
Preferably, described cicada slough extract solution is prepared using following methods:Cicada slough 20-25g is weighed, meal is ground into, plus
Enter the distilled water immersion 1-2h of 10-15 times of its weight, refluxing extraction 1-1.5h, suction filtration obtains filtrate 1;To 8-10 times of addition in filter residue
The distilled water of filter residue weight, refluxing extraction 1-1.5h, suction filtration obtains filtrate 2;Again to the 8-10 times of steaming of filter residue weight of addition in filter residue
Distilled water, refluxing extraction 1-1.5h, suction filtration obtains filtrate 3;Filtrate 1, filtrate 2, filtrate 3 are merged, is 40-50 DEG C, vacuum in temperature
Spend to be concentrated under reduced pressure into 4-6 times of cicada slough weight under the conditions of 0.05-0.08Pa, with three layers of filtered through gauze, obtain final product cicada slough extract solution.
Preferably, described fibroin solutions are silk fibroin solution.
Preferably, described membranoid substance is prepared using following methods:By fibroin solutions and fibroin nanofiber
Solution mixes, and adds the well mixed prepared spinning solution of cicada slough extract solution, Diclofenac, essential oil, spinning solution to use electrostatic spinning
Technique is obtained three-D non-woven net-structured film.
It is further preferred that described electrostatic spinning process is:Voltage 12-18KV, spinning height 5-10cm, winding speed
100-300rpm, spinning solution flow 2-5ml/h, 50-150 DEG C of heating-up temperature.
Preferably, fibre diameter is 50nm-20um in described three-D non-woven net-structured film, and interfiber pore is l-
500um, film thickness is 50nm-20mm.
Preferably, described essential oil is Rosa Damascana.
The beneficial effects of the present invention are:
1) tissue renovation material of the invention, relative to simple fibroin material, is the soft tissue repair material of low modulus
Material, with preferably rush vascularization and tissue repair function.
2) three-dimensional porous material for organizational project must have porosity higher, with interconnected three-dimensional knot
Structure, in order to the propagation of cell, migration, for the growth of cell provides enough nutriments.After adding nanofiber, improve
There are nanofibrous structures on the pore-creating character support hole wall of support, the specific surface area of support is considerably increased.
3) medicinal herb componentses cicada slough extract solution and Diclofenac, essential oil are added, materials microstructure reparation is further increased
Ability.
4) cicada slough can treat dissipate wind-heat, and facilitaing lung determines convulsion.Affection of exogenous wind-heat is controlled, cough sound is mute, measles without adequate eruption, rubella
Itch, pediatric epilepsy scared, hot eyes, ocular, furuncle swelling toxin, lockjaw.
Specific embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of fibroin tissue renovation material containing cicada slough, is the membranoid substance comprising fibroin, internal average Kong Mi
It is 1-3000/mm to spend2, average pore size is 1-500 μm, described raw material of the membranoid substance comprising following parts by weight:Silk egg
White 115 parts of solution, 7 parts of fibroin nanofiber solution, 2 parts of cicada slough extract solution, 1 part of Diclofenac, 1 part of essential oil.
Described fibroin nanofiber solution is prepared using following methods:Take fibroin solutions and be placed in 60 DEG C of constant temperature
The half of original volume is concentrated into case, then be transferred in fume hood be concentrated into mass fraction for 20% concentrate, concentrate is put
Enter that concentrate is diluted with water into mass fraction after 4 DEG C of refrigerator storage 2d is 0.5%, be put into insulating box after full and uniform stirring
It is incubated to the fibroin nanofiber solution for forming translucent gels shape.
The length of fibroin nanofiber is 1 μm, a diameter of 10- in described fibroin nanofiber solution
20nm。
Described fibroin solutions are prepared using following methods:2L deionized waters are heated to boiling, 4.24g carbon is added
Sour sodium and 5g silkworm raw silks, boil 30min, and after being cleaned up with deionized water, drying, the LiBr solution with 9.3mol/L is molten
Solution;Solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice,
Obtain fibroin solutions.
Described cicada slough extract solution is prepared using following methods:Cicada slough 20g is weighed, meal is ground into, its weight 10 is added
Distilled water immersion 1h again, refluxing extraction 1h, suction filtration obtains filtrate 1;To 8 times of distilled water of filter residue weight are added in filter residue, flow back
1h is extracted, suction filtration obtains filtrate 2;Again to 8 times of distilled water of filter residue weight of addition in filter residue, refluxing extraction 1h, suction filtration obtains filtrate 3;
Filtrate 1, filtrate 2, filtrate 3 are merged, temperature be 40 DEG C, vacuum be 0.05Pa under the conditions of be concentrated under reduced pressure into cicada slough weight
4 times, with three layers of filtered through gauze, obtain final product cicada slough extract solution.
Described fibroin solutions are silk fibroin solution.
Described membranoid substance is prepared using following methods:Fibroin solutions are mixed with fibroin nanofiber solution
Close, and add the well mixed prepared spinning solution of cicada slough extract solution, Diclofenac, essential oil, spinning solution to use electrostatic spinning process system
Obtain three-D non-woven net-structured film.
Described electrostatic spinning process is:Voltage 12-18KV, spinning height 5-10cm, winding speed 100-300rpm, spin
Silk flow quantity 2-5ml/h, 50-150 DEG C of heating-up temperature.
Fibre diameter is 50nm-20um in described three-D non-woven net-structured film, and interfiber pore is l-500um, film
Thickness is 50nm-20mm.
Described essential oil is Rosa Damascana.
Embodiment 2
A kind of fibroin tissue renovation material containing cicada slough, is the membranoid substance comprising fibroin, internal average Kong Mi
It is 1-3000/mm to spend2, average pore size is 1-500 μm, described raw material of the membranoid substance comprising following parts by weight:Silk egg
White 125 parts of solution, 8 parts of fibroin nanofiber solution, 3 parts of cicada slough extract solution, 2 parts of Diclofenac, 2 parts of essential oil.
Described fibroin nanofiber solution is prepared using following methods:Take fibroin solutions and be placed in 60 DEG C of constant temperature
The half of original volume is concentrated into case, then be transferred in fume hood be concentrated into mass fraction for 30% concentrate, concentrate is put
Enter that concentrate is diluted with water into mass fraction after 4 DEG C of refrigerator storage 2d is 0.5%, be put into insulating box after full and uniform stirring
It is incubated to the fibroin nanofiber solution for forming translucent gels shape.
The length of fibroin nanofiber is 1 μm, a diameter of 10- in described fibroin nanofiber solution
20nm。
Described fibroin solutions are prepared using following methods:2L deionized waters are heated to boiling, 4.24g carbon is added
Sour sodium and 5g silkworm raw silks, boil 30min, and after being cleaned up with deionized water, drying, the LiBr solution with 9.3mol/L is molten
Solution;Solution is loaded into bag filter until completely dissolved, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice,
Obtain fibroin solutions.
Described cicada slough extract solution is prepared using following methods:Cicada slough 25g is weighed, meal is ground into, its weight 15 is added
Distilled water immersion 2h again, refluxing extraction 1.5h, suction filtration obtains filtrate 1;To 10 times of distilled water of filter residue weight are added in filter residue, return
Stream extracts 1.5h, and suction filtration obtains filtrate 2;Again to 10 times of distilled water of filter residue weight of addition in filter residue, refluxing extraction 1.5h, suction filtration is obtained
Filtrate 3;Filtrate 1, filtrate 2, filtrate 3 are merged, temperature be 50 DEG C, vacuum be 0.08Pa under the conditions of be concentrated under reduced pressure into cicada slough
6 times of weight, with three layers of filtered through gauze, obtain final product cicada slough extract solution.
Described fibroin solutions are silk fibroin solution.
Described membranoid substance is prepared using following methods:Fibroin solutions are mixed with fibroin nanofiber solution
Close, and add the well mixed prepared spinning solution of cicada slough extract solution, Diclofenac, essential oil, spinning solution to use electrostatic spinning process system
Obtain three-D non-woven net-structured film.
Described electrostatic spinning process is:Voltage 12-18KV, spinning height 5-10cm, winding speed 100-300rpm, spin
Silk flow quantity 2-5ml/h, 50-150 DEG C of heating-up temperature.
Fibre diameter is 50nm-20um in described three-D non-woven net-structured film, and interfiber pore is l-500um, film
Thickness is 50nm-20mm.
Described essential oil is Rosa Damascana.
By tissue renovation material (S), tissue renovation material (SS) obtained in embodiment 2 obtained in embodiment 1, and by pure silk
The performance of the tissue renovation material (SSS) that fibroin is made is contrasted, using the little Bai with identical degree of tissue damage
Mouse is tested, and material S, SS and SSS are spread on the injury site of small white mouse respectively, is changed daily twice, continuous to use 10 days,
Using observation point system, small white mouse wounded tissue recovery extent is given a mark by 1-5 points respectively, every using effect is optimal to be
5 points, the experimental data for drawing is shown in Table 1.
The resultant effect of table 1 is investigated
Material | S | SS | SSS |
Tissue repair degree | 4.5 | 4.6 | 2.9 |
Antiphlogistic effects | 4.4 | 4.7 | 2.6 |
By experimental result it can be seen that:To being added in silk fibroin, fibroin nanofiber, cicada slough, double chlorine are fragrant
Acid, essential oil, can effectively lift tissue repairing ability and the antiphlogistic effects of fibroin albumen.
Claims (9)
1. a kind of fibroin tissue renovation material containing cicada slough, is the membranoid substance comprising fibroin, internal average cell density
It is 1-3000/mm2, average pore size is 1-500 μm, it is characterised in that original of the described membranoid substance comprising following parts by weight
Material:Fibroin solutions 115-125 parts, 7-8 parts of fibroin nanofiber solution, 2-3 parts of cicada slough extract solution, Diclofenac 1-
2 parts, essential oil 1-2 parts.
2. a kind of fibroin tissue renovation material containing cicada slough according to claim 1, it is characterised in that described silkworm
Silk-fibroin nanofiber solution is prepared using following methods:Take fibroin solutions and be placed in 60 DEG C of insulating boxs and be concentrated into original volume
Half, then be transferred in fume hood and be concentrated into mass fraction for 20-30% obtains concentrate, concentrate is put into 4 DEG C of refrigerator storages
It is 0.5% that concentrate is diluted with water into mass fraction after 2d, is put into after full and uniform stirring in insulating box and is incubated to formation half
The fibroin nanofiber solution of transparent gel-form.
3. a kind of fibroin tissue renovation material containing cicada slough according to claim 2, it is characterised in that:Described silkworm
The length of fibroin nanofiber is 1 μm, a diameter of 10-20nm in silk-fibroin nanofiber solution.
4. a kind of fibroin tissue renovation material containing cicada slough according to claim 1, it is characterised in that described silkworm
Silk protein solution is prepared using following methods:2L deionized waters are heated to boiling, 4.24g sodium carbonate and the life of 5g silkworms is added
Silk, boils 30min, after being cleaned up with deionized water, dry, with the LiBr solution dissolving of 9.3mol/L;Until completely dissolved
Solution is loaded into bag filter, deionized water dialysis 72h, 9000r/min centrifugation 20min is repeated twice, and obtains fibroin solutions.
5. a kind of fibroin tissue renovation material containing cicada slough according to claim 1, it is characterised in that described cicada
Extract solution is sloughed off to be prepared using following methods:Cicada slough 20-25g is weighed, meal is ground into, the distilled water of 10-15 times of its weight is added
Immersion 1-2h, refluxing extraction 1-1.5h, suction filtration obtains filtrate 1;To the 8-10 times of distilled water of filter residue weight is added in filter residue, backflow is carried
1-1.5h is taken, suction filtration obtains filtrate 2;Again to the 8-10 times of distilled water of filter residue weight of addition, refluxing extraction 1-1.5h, suction filtration in filter residue
Obtain filtrate 3;Filtrate 1, filtrate 2, filtrate 3 are merged, temperature be 40-50 DEG C, vacuum be 0.05-0.08Pa under the conditions of depressurize
4-6 times of cicada slough weight is concentrated into, with three layers of filtered through gauze, cicada slough extract solution is obtained final product.
6. a kind of fibroin tissue renovation material containing cicada slough according to claim 1, it is characterised in that:Described silkworm
Silk protein solution is silk fibroin solution.
7. a kind of fibroin tissue renovation material containing cicada slough according to claim 1, it is characterised in that described film
Shape thing is prepared using following methods:Fibroin solutions are mixed with fibroin nanofiber solution, and adds cicada slough to extract
The well mixed prepared spinning solution of liquid, Diclofenac, essential oil, spinning solution is obtained three-D non-woven network structure using electrostatic spinning process
Film.
8. a kind of fibroin tissue renovation material containing cicada slough according to claim 7, it is characterised in that described is quiet
Electrospinning process is:Voltage 12-18KV, spinning height 5-10cm, winding speed 100-300rpm, spinning solution flow 2-5ml/h,
50-150 DEG C of heating-up temperature.
9. a kind of fibroin tissue renovation material containing cicada slough according to claim 7, it is characterised in that:Described three
Fibre diameter is 50nm-20um in dimension nonwoven web film, and interfiber pore is 1-500um, and film thickness is 50nm-20mm.
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CN115581801A (en) * | 2022-09-29 | 2023-01-10 | 苏州大学 | Calcium phosphate mineralized silk micro-nano fiber membrane and preparation method thereof |
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CN113069593A (en) * | 2021-04-10 | 2021-07-06 | 中国人民解放军总医院 | Preparation process of silk fibroin hydrogel for premixed stem cell secretory protein group |
CN115581801A (en) * | 2022-09-29 | 2023-01-10 | 苏州大学 | Calcium phosphate mineralized silk micro-nano fiber membrane and preparation method thereof |
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