CN106967774A - A kind of shrimp med that ferments prepares the zymotechnique of astaxanthin - Google Patents
A kind of shrimp med that ferments prepares the zymotechnique of astaxanthin Download PDFInfo
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- CN106967774A CN106967774A CN201710329237.8A CN201710329237A CN106967774A CN 106967774 A CN106967774 A CN 106967774A CN 201710329237 A CN201710329237 A CN 201710329237A CN 106967774 A CN106967774 A CN 106967774A
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
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Abstract
The present invention prepares astaxanthin using above-mentioned technique fermentation shrimp med, and addition fermented oyster shell prepares natural calcium lactate wherein, shrimp med and oyster shell are crushed first, then culture medium and composite probiotics preparations are prepared, finally culture medium, composite probiotics preparations and oyster shell powder are well mixed and fermented.This method technique is simple, and rationally, the product produced can quickly supplement calcium, phosphorus and necessary trace element to process for breeding water body, can improve water body buffer capacity, stabilizing water quality, reduce environment to cultivated animals stress；Harden quickly shrimp crab crust, prevent the unsuccessful and soft shell that shells；Immunological regulation in animal body is participated in, disease-resistant and anti-stress ability is improved；Beneficial to shrimp crab liver toxin expelling, such as nitrite, ammonia nitrogen are poisoned；Promote the ejection of shrimp crab, growth.It is adapted to use before and after shrimp crab ejection, promotes duricrust, rejuvenate, prevent pathogen from invading.
Description
Technical field
The present invention relates to for biofermentation technique, specifically a kind of shrimp med that ferments prepares the zymotechnique of astaxanthin.
Background technology
Krill is that live in Southern Oceans a kind of belongs to Arthropoda, Crustachia, the zooplankter of Euphausiacea,
Its body length is generally 3-5 centimetres, and body color is transparent, with bronzing spot, using phytoplankton as food, in Southern Oceans food chain
In play an important role.The biomass of krill is very huge, conservative estimation, and the biomass of krill is about 6.5-10 hundred million
Ton, it is annual can amount of fishing be about hundred million tons of 0.65-1.0, the total amount of fishing of marine organisms of 1 year equivalent to the whole world.With global fishing
The generally decline of industry resource, the exploitation of krill resource are by the extensive concern of countries in the world, and China is populous nation,
Natural resources is relatively poor, and the comprehensive development and utilization of Antarctic krill has extremely important strategic importance.
Krill has that biometric storage amount is abundant, resource distribution extensively, the features such as be of high nutritive value, krill in addition
Or a huge marine resources protein pool.So in recent years, domestic and foreign scholars fall over each other to study the exploitation of krill resource
Exploitative potential.Contain crude protein it is reported that being up in the fresh sample of krill muscle containing crude protein in 16.31%, muscle dry sample
64.44%.Rich amino acids in its protein hydrolysate, it is essential comprising human body, and can not be synthesized in body
The 8 kinds of essential amino acids that must be supplemented from food:Methionine, lysine, valine, isoleucine, phenylalanine, bright ammonia
Acid, tryptophan, threonine.
About contain 3-4 mg/100g astaxanthins in krill crust.Astaxanthin is one kind in carotenoid, is had
Very strong antioxidation activity.It is also a kind of natural pigment simultaneously, and livestock rearing industry and water are applied to frequently as feed addictive
Produce aquaculture.
In krill body (full shrimp 3.37% is accounted for rich in mineral matter element）, mainly including phosphorus, calcium, zinc, iron, selenium etc..
Astaxanthin has pigmentation.Astaxanthin is the main carotenoids of pigment in marine crustacean and Fish
Element, it is due to astaxanthin in its a large amount of accumulation in vivo that the ruddy yellowish pink of marine product such as salmon, lobster, which is, but cultivation object itself nothing
Method synthesizing astaxanthin, and lack natural origin, it is therefore necessary to give and add in its feed, to play a part of supplementing pigment.
One of main purposes of current astaxanthin is, as the pigment source in aquaculture, to be initially applied to toadfish and fin fish, existing
It has been widely used in various cultivation objects.
Astaxanthin has antioxidation.In the molecular structure of astaxanthin, containing very long conjugated double bond, have hydroxyl and
Ketone group, these structures are the bases that astaxanthin has super oxidation resistance, astaxanthin can efficiently singlet-oxygen quenching, clearly
It is very unstable except internal free radical and anti-aging singlet oxygens and free radical contain non-matching electronics, it is very active and anti-
Answering property is extremely strong, can trigger chain reaction cause the phospholipid acid choline lipid peroxidation on biomembrane, protein denaturation degraded and
Nucleic acid is damaged.The oxidation resistance of astaxanthin is higher than 6 carrotene more than 10 times, be 500 times of vitamin E oxidation resistance with
On, enjoy " supper vitamin E " good reputation.
Astaxanthin can improve the ability of aquatic livestock resisting stress.Merchie etc. (1998) researchs find to add in feed
Plus astaxanthin can make the immunity of cultivation object obtain larger raising, strengthen premunition, improve survival rate, can also strengthen the later stage
The young weakens injury of the ultraviolet radioactive to aquatic livestock to the resistivity of salinity fluctuation.((2003) are tested to be shown Pan etc., bait
Astaxanthin is added in material can improve the anti-ammonia stress ability of Penaeus monodon.((2001) are it is also shown that astaxanthin can for their research
Increase drop banana prawn to heat stress and salinity stress resistivity
Astaxanthin can promote the growth of cultivation object, breeding and develop.Growth of the astaxanthin to cultivated animals remarkably promotes work
With.Jin Zhengyu (1999) etc. reports that addition astaxanthin can significantly improve the rate of body weight gain of Macrobrachium rosenbergii in feed.Petit etc.
(1997) research is found, feeding astaxanthin can shorten the moulting cycle of prawn postlarva.Astaxanthin but also as fertilization hormone,
Improve archiblast.Astaxanthin is added in feed, the survival rate of juvenile prawn and the ovum buoyancy and survival rate of fish can be improved, educated in salmon
Seedling stage can also increase the survival rate and growth rate of rate of fertilization, ovum, and protection ovum resists the influence of mal-condition, and promotes the maturation of ovum
With raising fecundity, embryonic death is reduced.
Astaxanthin can improve the physiological function of cultivation object.Astaxanthin is added in feed can improve cultivation rainbow trout health
Situation, can make it have more preferable liver function, can also strengthen the structure and glycogen storage .Rehulka of red tilapia liver cell
(2000) influence of the astaxanthin to various indexs and part physiological function in the growth rate of rainbow trout, blood, hair be have studied
Now by feeding astaxanthin, the hematopoiesis function and lipoid of rainbow trout and the metabolic function of calcium can be improved.
Addition astaxanthin is easy to aquatic product transportation and preservation.In the transportation of aquatic products, the oxidation of lipid is meat
The main cause of corruption, astaxanthin can protect lipid material to exempt from oxidation, and this is embodied in the transport and preservation of aquatic products
Positive role .Jesnsen etc. (X998) is carried out to anti-oxidation function of the carotenoid such as astaxanthin in aquatic product cold storage freshness preservation
Research, as a result shows, the rainbow trout lipid oxidation for feeding low concentration astaxanthin is serious, and feed high concentration astaxanthin then can be in raw meat
Preservation is played in refrigeration.In addition, adding astaxanthin in feed, increase its content in aquaculture subject, can
To reduce the use of chemical preservative to a certain extent, and as a kind of special, efficient " biological preservative ", make aquatic products
Product are stored more long.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of technique simply, and process is reasonable, by biofermentation technique,
Astaxanthin in shrimp med is discharged, and the inorganic calcium existed in oyster shell with calcium carbonate form is changed into calcium activated,
The lot of trace such as the protein such as L-aminobutanedioic acid, glycine, isoleucine and iron, sodium, zinc, selenium, strontium, copper member is discharged simultaneously
Element, additionally it is possible to improve the quantity of probiotics in water body, and strain used is especially tamed through seawater, is adapted to sea-farming ring
Border, bacterium is mutually stablized, efficiently.
The present invention solve above-mentioned technical problem use technical scheme be：A kind of shrimp med that ferments prepares the fermentation work of astaxanthin
Skill, it is characterised in that：
Concrete technology step is：
A. the selection and pretreatment of raw material：The shrimp med of high-quality is chosen, 200-250 mesh is crushed to, the oyster shell of high-quality is chosen, clearly
It is crushed to 200-250 mesh by wash clean, drying respectively, places standby at aeration-drying；
B. the preparation of culture medium：Sal-ammoniac (NH4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate
(CH3COONa):Magnesium chloride (MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water：Seawater=50:15:15:20:6:20:1000:
9437：9437, it is standby that nutrient solution is made in fully dissolving；
C. beautiful plain preparation：Take nutrient solution obtained by probiotics and step b by volume 1:7-8 ratio pours into fermentation tank
In, 5-10 parts of shrimp med in step a is added, 5-10 parts of oyster shell powder stirs, shrimp med and oyster shell powder is fully dissolved.Protect
It is 25-30 DEG C to hold fermentation temperature, and beautiful element is made in anaerobic fermentation culture 6-10 days.
The present invention prepares astaxanthin using above-mentioned technique fermentation shrimp med, and addition fermented oyster shell prepares genuine milk wherein
Sour calcium, shrimp med and oyster shell are crushed, then prepare culture medium and composite probiotics preparations first, finally by culture medium, compound
Probiotics preparation and oyster shell powder, which are well mixed, to be fermented.This method technique is simple, and rationally, the product produced can for process
Think that breeding water body quickly supplements calcium, phosphorus and necessary trace element, water body buffer capacity can be improved, stabilizing water quality is reduced
Environment stress to cultivated animals；Harden quickly shrimp crab crust, prevent the unsuccessful and soft shell that shells；Participate in exempting from animal body
Epidemic disease is adjusted, and improves disease-resistant and anti-stress ability；Beneficial to shrimp crab liver toxin expelling, such as nitrite, ammonia nitrogen are poisoned；Shrimp crab is promoted to move back
Shell, growth.It is adapted to use before and after shrimp crab ejection, promotes duricrust, rejuvenate, prevent pathogen from invading；Also promote water body unit cell
Algae and beneficial microorganism growth and breeding, reduce poor environment（Rain, blow, the temperature difference is big etc.）The influence of algae and water；Harvest
Before splash, shrimp and crab shells can be made to be hardened, brighten, improving quality.
Embodiment
With reference to embodiment, the invention will be further described.
The present invention is the zymotechnique that a kind of shrimp med that ferments prepares astaxanthin, and it comprises the following steps：
A. the selection and pretreatment of raw material：The shrimp med of high-quality is chosen, 200-250 mesh is crushed to, the oyster shell of high-quality is chosen, clearly
It is crushed to 200-250 mesh by wash clean, drying respectively, places standby at aeration-drying；
B. the preparation of culture medium：Sal-ammoniac (NH4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate
(CH3COONa):Magnesium chloride (MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water：Seawater=50:15:15:20:6:20:1000:
9437：9437, it is standby that nutrient solution is made in fully dissolving；
C. beautiful plain preparation：Take nutrient solution obtained by probiotics and step b by volume 1:7-8 ratio pours into fermentation tank
In, 5-10 parts of shrimp med in step a is added, 5-10 parts of oyster shell powder stirs, shrimp med and oyster shell powder is fully dissolved.Protect
It is 25-30 DEG C to hold fermentation temperature, and beautiful element is made in anaerobic fermentation culture 6-10 days.
Embodiment 1：
A kind of shrimp med that ferments prepares the zymotechnique of astaxanthin, and it comprises the following steps：
A. the selection and pretreatment of raw material：The shrimp med of high-quality is chosen, 200 mesh are crushed to, the oyster shell of high-quality is chosen, cleaning is dry
Only, dry, it is crushed to 200 mesh respectively, place standby at aeration-drying；
B. the preparation of culture medium：Sal-ammoniac (NH4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate
(CH3COONa):Magnesium chloride (MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water：Seawater=50:15:15:20:6:20:1000:
9437：9437, it is standby that nutrient solution is made in fully dissolving；
C. beautiful plain preparation：Take nutrient solution obtained by probiotics and step b by volume 1:7 ratio pours into fermentation tank
In, 5 parts of shrimp med in step a is added, 5 parts of oyster shell powder stirs, shrimp med and oyster shell powder is fully dissolved.Keep fermentation
Temperature is 25 DEG C, and beautiful element is made in anaerobic fermentation culture 6 days.
Example 2：
A. the selection and pretreatment of raw material：The shrimp med of high-quality is chosen, 250 mesh are crushed to, the oyster shell of high-quality is chosen, cleaning is dry
Only, dry, it is crushed to 250 mesh respectively, place standby at aeration-drying；
B. the preparation of culture medium：Sal-ammoniac (NH4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate
(CH3COONa):Magnesium chloride (MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water：Seawater=50:15:15:20:6:20:1000:
9437：9437, it is standby that nutrient solution is made in fully dissolving；
C. beautiful plain preparation：Take nutrient solution obtained by probiotics and step b by volume 1:7 ratio pours into fermentation tank
In, 8 parts of shrimp med in step a is added, 8 parts of oyster shell powder stirs, shrimp med and oyster shell powder is fully dissolved.Keep fermentation
Temperature is 28 DEG C, and beautiful element is made in anaerobic fermentation culture 8 days.
Example 3：
A. the selection and pretreatment of raw material：The shrimp med of high-quality is chosen, 250 mesh are crushed to, the oyster shell of high-quality is chosen, cleaning is dry
Only, dry, it is crushed to 250 mesh respectively, place standby at aeration-drying；
B. the preparation of culture medium：Sal-ammoniac (NH4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate
(CH3COONa):Magnesium chloride (MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water：Seawater=50:15:15:20:6:20:1000:
9437：9437, it is standby that nutrient solution is made in fully dissolving；
C. beautiful plain preparation：Take nutrient solution obtained by probiotics and step b by volume 1:8 ratio pours into fermentation tank
In, 10 parts of shrimp med in step a is added, 10 parts of oyster shell powder stirs, shrimp med and oyster shell powder is fully dissolved.Keep hair
Ferment temperature is 28 DEG C, and beautiful element is made in anaerobic fermentation culture 10 days.
Claims (4)
1. a kind of shrimp med that ferments prepares the zymotechnique of astaxanthin, it is characterised in that：It comprises the following steps：
A. the selection and pretreatment of raw material：The shrimp med of high-quality is chosen, 200-250 mesh is crushed to, the oyster shell of high-quality is chosen, clearly
It is crushed to 200-250 mesh by wash clean, drying respectively, places standby at aeration-drying；
B. the preparation of culture medium：Sal-ammoniac (NH4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate
(CH3COONa):Magnesium chloride (MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water：Seawater=50:15:15:20:6:20:1000:
9437：9437, it is standby that nutrient solution is made in fully dissolving；
C. beautiful plain preparation：Take nutrient solution obtained by probiotics and step b by volume 1:7-8 ratio pours into fermentation tank
In, 5-10 parts of shrimp med in step a is added, 5-10 parts of oyster shell powder stirs, shrimp med and oyster shell powder is fully dissolved, protected
It is 25-30 DEG C to hold fermentation temperature, and beautiful element is made in anaerobic fermentation culture 6-10 days.
2. fermentation shrimp med according to claim 1 prepares the zymotechnique of astaxanthin, it is characterised in that：In the step a
The shrimp med of high-quality is chosen, 200-250 mesh is crushed to, the oyster shell of high-quality is chosen, cleans up, dries, it is crushed to respectively
200-250 mesh, places standby at aeration-drying.
3. fermentation shrimp med according to claim 1 prepares the zymotechnique of astaxanthin, it is characterised in that：In the step b
Sal-ammoniac (NH4Cl):Sodium acid carbonate (NaHCO3):Dipotassium hydrogen phosphate (K2HPO4):Sodium acetate (CH3COONa):Magnesium chloride
(MgCl2):Sodium chloride (NaCl):Corn flour:Fresh water：Seawater=50:15:15:20:6:20:1000:9437：9437, it is fully molten
Solution, is made nutrient solution standby.
4. fermentation shrimp med according to claim 1 prepares the zymotechnique of astaxanthin, it is characterised in that：In the step c
Take nutrient solution obtained by probiotics and step b by volume 1:7-8 ratio is poured into fermentation tank, adds shrimp med in step a
5-10 parts, 5-10 parts of oyster shell powder stirs, shrimp med and oyster shell powder is fully dissolved, and holding fermentation temperature is 25-30
DEG C, beautiful element is made in anaerobic fermentation culture 6-10 days.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101215595A (en) * | 2007-12-26 | 2008-07-09 | 大连工业大学 | Method for extracting astaxanthin, protein and chitin from shrimp shell by utilizing microorganism |
| CN102586378A (en) * | 2012-03-12 | 2012-07-18 | 中国海洋大学 | Method for extracting substances from fermented shrimp head shells |
| CN103898016A (en) * | 2014-03-25 | 2014-07-02 | 华中农业大学 | High-yield lactic acid bacterium and method for preparing calcium lactate by fermenting eggshells with same |
-
2017
- 2017-05-11 CN CN201710329237.8A patent/CN106967774A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101215595A (en) * | 2007-12-26 | 2008-07-09 | 大连工业大学 | Method for extracting astaxanthin, protein and chitin from shrimp shell by utilizing microorganism |
| CN102586378A (en) * | 2012-03-12 | 2012-07-18 | 中国海洋大学 | Method for extracting substances from fermented shrimp head shells |
| CN103898016A (en) * | 2014-03-25 | 2014-07-02 | 华中农业大学 | High-yield lactic acid bacterium and method for preparing calcium lactate by fermenting eggshells with same |
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Application publication date: 20170721 |