CN106962926A - To discard the method that Chinese caterpillar fungus culture medium prepares ferment as raw material - Google Patents
To discard the method that Chinese caterpillar fungus culture medium prepares ferment as raw material Download PDFInfo
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- CN106962926A CN106962926A CN201710296323.3A CN201710296323A CN106962926A CN 106962926 A CN106962926 A CN 106962926A CN 201710296323 A CN201710296323 A CN 201710296323A CN 106962926 A CN106962926 A CN 106962926A
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- Prior art keywords
- kilogram
- culture medium
- caterpillar fungus
- chinese caterpillar
- fungus culture
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- 239000001963 growth medium Substances 0.000 title claims abstract description 37
- 241000233866 Fungi Species 0.000 title claims abstract description 36
- 240000001307 Myosotis scorpioides Species 0.000 title claims abstract description 36
- 239000002994 raw material Substances 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 10
- 241000228212 Aspergillus Species 0.000 claims abstract description 13
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 13
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 11
- 241000894006 Bacteria Species 0.000 claims abstract description 8
- 241000205585 Aquilegia canadensis Species 0.000 claims abstract description 7
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 7
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 7
- 241000196251 Ulva arasakii Species 0.000 claims abstract description 7
- 239000002131 composite material Substances 0.000 claims abstract description 7
- 235000001405 Artemisia annua Nutrition 0.000 claims abstract description 6
- 240000000011 Artemisia annua Species 0.000 claims abstract description 6
- 244000078782 Brassica arvensis Species 0.000 claims abstract description 6
- 241001264174 Cordyceps militaris Species 0.000 claims abstract description 4
- 244000017020 Ipomoea batatas Species 0.000 claims description 11
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 11
- 239000001888 Peptone Substances 0.000 claims description 11
- 108010080698 Peptones Proteins 0.000 claims description 11
- 238000000855 fermentation Methods 0.000 claims description 11
- 230000004151 fermentation Effects 0.000 claims description 11
- 235000019319 peptone Nutrition 0.000 claims description 11
- 229920001661 Chitosan Polymers 0.000 claims description 9
- 238000004108 freeze drying Methods 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 claims 1
- 239000004382 Amylase Substances 0.000 abstract description 2
- 102000013142 Amylases Human genes 0.000 abstract description 2
- 108010065511 Amylases Proteins 0.000 abstract description 2
- 108010059892 Cellulase Proteins 0.000 abstract description 2
- 102000004882 Lipase Human genes 0.000 abstract description 2
- 239000004367 Lipase Substances 0.000 abstract description 2
- 108090001060 Lipase Proteins 0.000 abstract description 2
- 108091005804 Peptidases Proteins 0.000 abstract description 2
- 239000004365 Protease Substances 0.000 abstract description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract description 2
- 235000019418 amylase Nutrition 0.000 abstract description 2
- 229940106157 cellulase Drugs 0.000 abstract description 2
- 235000019421 lipase Nutrition 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 229940088594 vitamin Drugs 0.000 abstract description 2
- 229930003231 vitamin Natural products 0.000 abstract description 2
- 235000013343 vitamin Nutrition 0.000 abstract description 2
- 239000011782 vitamin Substances 0.000 abstract description 2
- 150000003722 vitamin derivatives Chemical class 0.000 abstract description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 abstract 1
- 108010012715 Superoxide dismutase Proteins 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 2
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 240000001851 Artemisia dracunculus Species 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241001674939 Caulanthus Species 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 230000006819 RNA synthesis Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 208000013116 chronic cough Diseases 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 210000000629 knee joint Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 150000002927 oxygen compounds Chemical class 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/40—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
- A23L3/44—Freeze-drying
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of to discard the method that Chinese caterpillar fungus culture medium prepares ferment as raw material, it is related to Cordyceps militaris preparing technical field；The present invention realizes the comprehensive utilization of resource using discarded Chinese caterpillar fungus culture medium as raw material；Folium artemisiae argyi, honeysuckle, agaric, sea lettuce, tealeaves, dregs of beans, sweet wormwood, violet cabbage are with the addition of into discarded Chinese caterpillar fungus culture medium, using saccharomycete, Bifidobacterium and aspergillus as composite bacteria, abundant protease, lipase, superoxide dismutase, cellulase, amylase vitamin, polysaccharose substance etc. are generated.
Description
Technical field
It is especially a kind of to prepare ferment using discarded Chinese caterpillar fungus culture medium as raw material the present invention relates to ferment preparing technical field
Method.
Background technology
Often contain cordycepic acid and cordycepin in discarded Chinese caterpillar fungus culture medium, cordycepic acid enters lung kidney two warp, can tonifying lung it is cloudy,
Energy kidney-replenishing, cures mainly kidney deficiency again, and impotence and seminal emission, soreness of waist and knee joint, eak after being ill, chronic cough is weak, phthisical cough phlegm blood, spontaneous sweating etc.,
Be it is unique it is a kind of can be while balancing, adjusting the Chinese medicine of negative and positive.Cordycepin in Cordyceps militaris has antiviral, antibacterial, substantially suppression
Tumour growth processed, effect of interference human body RNA and DNA synthesis；But after existing Cordyceps militaris harvest, generally by culture medium
Abandon, cause the waste of resource, therefore, comprehensive exploitation, the circulation of resource can be realized using discarded Chinese caterpillar fungus culture medium
Utilize.
The content of the invention
It is this with useless it is an object of the invention to provide a kind of to discard the method that Chinese caterpillar fungus culture medium prepares ferment as raw material
Abandon the problem of Chinese caterpillar fungus culture medium can solve how to comprehensively utilize discarded Chinese caterpillar fungus culture medium for the method that raw material prepares ferment.
In order to solve the above problems, the technical solution adopted by the present invention is：
This method for preparing ferment as raw material to discard Chinese caterpillar fungus culture medium comprises the following steps：
Using 1 kilogram of discarded Chinese caterpillar fungus culture medium as raw material, crush, the quality added after crushing is 0.2 kilogram~0.3 kilogram
Folium artemisiae argyi, honeysuckle, agaric, sea lettuce, tealeaves, dregs of beans, sweet wormwood, violet cabbage, 0.05 kilogram then added~0.06 kilogram of shell
Glycan, 0.01 kilogram~0.02 kilogram peptone, 0.02 kilogram~0.03 kilogram sweet potato mud, are well mixed, and add saccharomycete, double
Discrimination bacillus and the composite bacteria of aspergillus composition, normal temperature fermentation 10 days~13 days；Continue to ferment 10 days~25 days after centrifugation, filtering,
Obtain an enzyme stoste；Then add 0.02 kilogram~0.03 kilogram chitosan, 0.01 kilogram~0.02 kilogram peptone,
0.02 kilogram~0.03 kilogram sweet potato mud, normal temperature fermentation 40 days~60 days is concentrated, freeze-drying；The wherein addition of saccharomycete
To discard the 0.02%~0.05% of Chinese caterpillar fungus culture medium quality, the addition of Bifidobacterium is discarded Chinese caterpillar fungus culture medium quality
0.005%~0.01%, the addition of aspergillus is the 0.005%~0.015% of discarded Chinese caterpillar fungus culture medium quality.
It is above-mentioned to discard in the technical scheme of Chinese caterpillar fungus culture medium method that ferment is prepared as raw material, more specifically technical side
Case can also be：It is -40 DEG C~-20 DEG C to be freeze-dried temperature.
By adopting the above-described technical solution, the present invention has the advantages that compared with prior art：
1st, the present invention realizes the comprehensive utilization of resource using discarded Chinese caterpillar fungus culture medium as raw material.
2nd, the present invention with the addition of folium artemisiae argyi, honeysuckle, agaric, sea lettuce, tealeaves, dregs of beans, green grass or young crops into discarded Chinese caterpillar fungus culture medium
Wormwood artemisia, violet cabbage, using saccharomycete, Bifidobacterium and aspergillus as composite bacteria, generate abundant protease, lipase, super oxygen
Compound mutase, cellulase, amylase vitamin, polysaccharose substance etc..
3rd, raw material of the present invention with the addition of chitosan, peptone, sweet potato mud, these three materials, be saccharomycete, Bifidobacterium and
The material that aspergillus has a passion for, during the fermentation, it is to avoid these three bacterium are using zymotic fluid or using ferment in raw material as battalion
The problem of product ferment content caused by foster material is low.
Embodiment
With reference to embodiment, the invention will be further described：
Embodiment 1
The present embodiment is crushed using 1 kilogram of discarded Chinese caterpillar fungus culture medium as raw material, 0.2 kilogram of folium artemisiae argyi, honeysuckle after addition crushing
0.3 kilogram, 0.2 kilogram of agaric, 0.3 kilogram of sea lettuce, 0.25 kilogram of tealeaves, 0.2 kilogram of dregs of beans, 0.2 kilogram of sweet wormwood, violet cabbage
0.3 kilogram, the 0.05 kilogram of chitosan then added, 0.02 kilogram of peptone, 0.027 kilogram of sweet potato mud are well mixed, plus
Enter the composite bacteria of saccharomycete, Bifidobacterium and aspergillus composition, normal temperature fermentation 13 days；Continue to ferment 25 days after centrifugation, filtering,
Obtain an enzyme stoste；Then add 0.02 kilogram~0.03 kilogram chitosan, 0.01 kilogram~0.02 kilogram peptone,
0.03 kilogram of sweet potato mud, normal temperature fermentation 40 days is concentrated, freeze-drying, and freeze-drying temperature is -40 DEG C~-20 DEG C.
The addition of the present embodiment saccharomycete is the 0.05% of discarded Chinese caterpillar fungus culture medium quality, the present embodiment Bifidobacterium
Addition be the 0.008% of discarded Chinese caterpillar fungus culture medium quality, the addition of the present embodiment aspergillus is discarded Chinese caterpillar fungus culture medium
The 0.005% of quality.
Embodiment 2
The present embodiment is crushed using 1 kilogram of discarded Chinese caterpillar fungus culture medium as raw material, 0.3 kilogram of folium artemisiae argyi, honeysuckle after addition crushing
0.25 kilogram, it is 0.3 kilogram of agaric, 0.25 kilogram of sea lettuce, 0.2 kilogram of tealeaves, 0.25 kilogram of dregs of beans, 0.27 kilogram of sweet wormwood, purple
0.2 kilogram of wild cabbage, the 0.053 kilogram of chitosan then added, 0.01 kilogram of peptone, 0.02 kilogram of sweet potato mud, mixing is equal
It is even, add the composite bacteria of saccharomycete, Bifidobacterium and aspergillus composition, normal temperature fermentation 11 days；Continue to ferment after centrifugation, filtering
15 days, obtain an enzyme stoste；Then 0.03 kilogram of chitosan, 0.01 kilogram~0.02 kilogram peptone, 0.02 are added
Kilogram sweet potato mud, normal temperature fermentation 47 days is concentrated, freeze-drying, and freeze-drying temperature is -40 DEG C~-20 DEG C.
The addition of the present embodiment saccharomycete is the 0.02% of discarded Chinese caterpillar fungus culture medium quality, the present embodiment Bifidobacterium
Addition be the 0.01% of discarded Chinese caterpillar fungus culture medium quality, the addition of the present embodiment aspergillus is discarded Chinese caterpillar fungus culture medium
The 0.015% of quality.
Embodiment 3
The present embodiment is crushed using 1 kilogram of discarded Chinese caterpillar fungus culture medium as raw material, 0.25 kilogram of folium artemisiae argyi, honeysuckle after addition crushing
0.2 kilogram, 0.25 kilogram of agaric, 0.3 kilogram of sea lettuce, 0.3 kilogram of tealeaves, 0.3 kilogram of dregs of beans, 0.3 kilogram of sweet wormwood, violet cabbage
0.24 kilogram, the 0.06 kilogram of chitosan then added, 0.017 kilogram of peptone, 0.03 kilogram of sweet potato mud are well mixed,
Add the composite bacteria of saccharomycete, Bifidobacterium and aspergillus composition, normal temperature fermentation 10 days；Continue to ferment 25 days after centrifugation, filtering,
Obtain an enzyme stoste；Then add 0.024 kilogram of chitosan, 0.01 kilogram~0.02 kilogram peptone, 0.02 kilogram
~0.03 kilogram of sweet potato mud, normal temperature fermentation 60 days is concentrated, freeze-drying, and freeze-drying temperature is -40 DEG C~-20 DEG C.
The addition of the present embodiment saccharomycete is the 0.03% of discarded Chinese caterpillar fungus culture medium quality, the present embodiment Bifidobacterium
Addition be the 0.005% of discarded Chinese caterpillar fungus culture medium quality, the addition of the present embodiment aspergillus is discarded Chinese caterpillar fungus culture medium
The 0.01% of quality.
Claims (2)
1. it is a kind of to discard the method that Chinese caterpillar fungus culture medium prepares ferment as raw material, it is characterised in that to comprise the following steps：
Using 1 kilogram of discarded Chinese caterpillar fungus culture medium as raw material, crush, the quality added after crushing is 0.2 kilogram~0.3 kilogram
Folium artemisiae argyi, honeysuckle, agaric, sea lettuce, tealeaves, dregs of beans, sweet wormwood, violet cabbage, 0.05 kilogram then added~0.06 kilogram of shell
Glycan, 0.01 kilogram~0.02 kilogram peptone, 0.02 kilogram~0.03 kilogram sweet potato mud, are well mixed, and add saccharomycete, double
Discrimination bacillus and the composite bacteria of aspergillus composition, normal temperature fermentation 10 days~13 days；Continue to ferment 10 days~25 days after centrifugation, filtering,
Obtain an enzyme stoste；Then add 0.02 kilogram~0.03 kilogram chitosan, 0.01 kilogram~0.02 kilogram peptone,
0.02 kilogram~0.03 kilogram sweet potato mud, normal temperature fermentation 40 days~60 days is concentrated, freeze-drying；Wherein described saccharomycete adds
Dosage is the 0.02%~0.05% of discarded Chinese caterpillar fungus culture medium quality, and the addition of the Bifidobacterium is discarded Cordyceps militaris culture
The 0.005%~0.01% of matrix amount, the addition of the aspergillus is the 0.005%~0.015% of discarded Chinese caterpillar fungus culture medium quality.
2. it is according to claim 1 to discard the method that Chinese caterpillar fungus culture medium prepares ferment as raw material, it is characterised in that cold
It is -40 DEG C~-20 DEG C to freeze drying temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710296323.3A CN106962926A (en) | 2017-04-28 | 2017-04-28 | To discard the method that Chinese caterpillar fungus culture medium prepares ferment as raw material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710296323.3A CN106962926A (en) | 2017-04-28 | 2017-04-28 | To discard the method that Chinese caterpillar fungus culture medium prepares ferment as raw material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106962926A true CN106962926A (en) | 2017-07-21 |
Family
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Family Applications (1)
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| CN201710296323.3A Withdrawn CN106962926A (en) | 2017-04-28 | 2017-04-28 | To discard the method that Chinese caterpillar fungus culture medium prepares ferment as raw material |
Country Status (1)
| Country | Link |
|---|---|
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108484291A (en) * | 2018-06-08 | 2018-09-04 | 沈阳中博环保科技研发有限公司 | Discarded Cordyceps militaris rice cake bio-bacterial manure and production method |
| CN113462725A (en) * | 2021-07-15 | 2021-10-01 | 深圳听研生物科技有限公司 | Cordyceps militaris secondary metabolite of sweet wormwood herb extract and preparation method and application thereof |
| CN114214370A (en) * | 2022-02-21 | 2022-03-22 | 南京昊禾生物科技有限公司 | Method for improving organic acid production efficiency of aspergillus |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1382788A (en) * | 2001-04-21 | 2002-12-04 | 汪景山 | Application of culture medium in artificial culture of cordyceps |
| CN102669658A (en) * | 2012-04-23 | 2012-09-19 | 浙江科技学院 | Preparation method of plant enzyme food |
| CN104856015A (en) * | 2015-04-24 | 2015-08-26 | 西藏月王生物技术有限公司 | A cordyceps sinensis enzyme and a preparation method thereof |
-
2017
- 2017-04-28 CN CN201710296323.3A patent/CN106962926A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1382788A (en) * | 2001-04-21 | 2002-12-04 | 汪景山 | Application of culture medium in artificial culture of cordyceps |
| CN102669658A (en) * | 2012-04-23 | 2012-09-19 | 浙江科技学院 | Preparation method of plant enzyme food |
| CN104856015A (en) * | 2015-04-24 | 2015-08-26 | 西藏月王生物技术有限公司 | A cordyceps sinensis enzyme and a preparation method thereof |
Non-Patent Citations (3)
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| 李冬生等: "《食品高新技术》", 30 November 2007, 中国计量出版社 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108484291A (en) * | 2018-06-08 | 2018-09-04 | 沈阳中博环保科技研发有限公司 | Discarded Cordyceps militaris rice cake bio-bacterial manure and production method |
| CN113462725A (en) * | 2021-07-15 | 2021-10-01 | 深圳听研生物科技有限公司 | Cordyceps militaris secondary metabolite of sweet wormwood herb extract and preparation method and application thereof |
| CN113462725B (en) * | 2021-07-15 | 2023-08-04 | 深圳听研生物科技有限公司 | Cordyceps militaris secondary metabolite of sweet wormwood herb extract as well as preparation method and application thereof |
| CN114214370A (en) * | 2022-02-21 | 2022-03-22 | 南京昊禾生物科技有限公司 | Method for improving organic acid production efficiency of aspergillus |
| CN114214370B (en) * | 2022-02-21 | 2022-06-17 | 南京昊禾生物科技有限公司 | Method for improving organic acid production efficiency of aspergillus |
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Application publication date: 20170721 |
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