CN106918582A - Microbial detection device - Google Patents
Microbial detection device Download PDFInfo
- Publication number
- CN106918582A CN106918582A CN201710209407.9A CN201710209407A CN106918582A CN 106918582 A CN106918582 A CN 106918582A CN 201710209407 A CN201710209407 A CN 201710209407A CN 106918582 A CN106918582 A CN 106918582A
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- China
- Prior art keywords
- display device
- detection device
- culture apparatus
- culture
- ccd camera
- Prior art date
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- 238000001514 detection method Methods 0.000 title claims abstract description 28
- 230000000813 microbial effect Effects 0.000 title claims abstract description 20
- 230000003287 optical effect Effects 0.000 claims abstract description 43
- 238000005070 sampling Methods 0.000 claims abstract description 18
- 238000001914 filtration Methods 0.000 claims 1
- 238000000034 method Methods 0.000 description 13
- 244000005700 microbiome Species 0.000 description 12
- 239000000523 sample Substances 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000000149 argon plasma sintering Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000001215 fluorescent labelling Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000029918 bioluminescence Effects 0.000 description 1
- 238000005415 bioluminescence Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of microbial detection device, its culture apparatus includes transparent casing and case lid, and multiple culture tanks are set on casing, and the bottom of casing embeds fluorescent optical sensor at culture tank lower section；Optical sampling component, optical sampling component includes main body frame, LASER Light Source, CCD camera and display device, main body frame upper end is uncovered structure, lower end closed and have round-meshed columnar structured, LASER Light Source irradiates located at one end of main body frame closing to uncovered structure one end, CCD camera is on circular hole, and CCD camera is connected with display device；Board is provided with display device installing port and culture apparatus mounting table, and the groove for placing culture apparatus is offered on culture apparatus mounting table, and groove is located at directly over optical sampling component, and display device is on display device installing port.
Description
Technical field
The present invention relates to microorganism detection field, more particularly to a kind of microbial detection device.
Background technology
With expanding economy, the continuous improvement of people's living standard, the consciousness for food security is also carried constantly
It is high.At present, the method for the detection of microorganism, including use immune measurement method, ELISA method, gas-chromatography mass analysis, liquid phase
The machines such as chromatogram mass analysis survey method, but the pre-treatment for determining is numerous and diverse, and the precision of judgement can not be said fully.These detection sides
Method before testing, is required to cultivate in advance, that is, spend and go culture more for a long time including the microorganism including spoilage organisms.
Using the technology including marking microorganism, to protrude between the light that microorganism sends and the light that growth holder sends
Contrast.Due to the sensitive optical system of the feature (such as wavelength or intensity) using the light to sending, so using specific
Fluorescence labeling, fluorogen or using the enzyme that can expose the bioluminescence sent by such as ATP, can carry out early stage to microorganism
Detection.
Additionally, it is most common and one of most ancient method to be cultivated by agar medium, wherein culture medium is for one
Plant or several microorganism types are more or less selectable.In a period of it generally can extend to several days, the culture medium is suitable
Cultivated at a temperature of, for growing the microorganism to be found.The shortcoming of this cultural method is still to be formed on agar
Bacterium colony can carry out the visible detection of bore hole, it is necessary to relatively long cultivation cycle.Be will also realize that from current technological level, can be with
Chain reaction (also referred to as PCR breedings) is performed by the breeding of DNA sequence dna or RNA sequence, so that it is determined that existing in sample specific
Microorganism.The shortcoming of these methods is：In order that the bacterium colony formed on agar medium can carry out the visible detection of bore hole, need
Want relatively long cultivation cycle.And be will also realize that from current technological level, can be by the breeding of NDA sequences or RNA sequence
Chain polymerization reaction (also referred to as PCR breedings) is performed, so that it is determined that the specified microorganisms in sample.The shortcoming of these methods is desirable
Several DNA, i.e., several microorganism generally requires at least tens kinds microorganisms, and this method is not based on the side of growth
Method is sensitive.
Above-mentioned agar medium or nutrient solution are cultivated, and look into method by vision or fluorescence labeling is identified, and
Visual examination technique includes the turbidity and color change of the suspended material in monitoring nutrient solution, and the efficiency comparison of the two methods is low
Under, and can only rough Statistics, accuracy rate is less high.
The content of the invention
It is an object of the invention to provide a kind of microbial detection device.
To achieve these goals, the technical scheme of present invention offer is：A kind of microbial detection device is provided, including：
Culture apparatus, the culture apparatus includes transparent casing and case lid, and multiple culture tanks are set on the casing, and
The bottom of the casing is located at culture tank lower section and embeds fluorescent optical sensor；
Optical sampling component, the optical sampling component includes main body frame, LASER Light Source, CCD camera and display dress
Put, the main body frame upper end be uncovered structure, lower end closed and have round-meshed columnar structured, the LASER Light Source is located at
One end of main body frame closing and irradiated to described uncovered structure one end, the CCD camera on the circular hole, and
The CCD camera is connected with the display device；
Board, the board is provided with display device installing port and culture apparatus mounting table, on the culture apparatus mounting table
The groove for placing the culture apparatus is offered, the groove is located at directly over the optical sampling component, the display
Device is on the display device installing port.
The groove is located at the surface of the uncovered structure.
The case lid just to being provided with boss at the culture tank, by the boss realized and the culture tank by the case lid
Be tightly connected.
Light source optical filter is additionally provided with the LASER Light Source.
Launching light optical filter and optical system are additionally provided with the CCD camera, the light source optical filter and launching light are filtered
Mating plate filters the light of same frequency, and the CCD camera realizes the inspection to the fluorescent optical sensor by the optical system
Survey.
The coarse structure of the main body frame inner surface for the benefit of light scattering.
The casing edge is provided with locating slot, and case lid described in the correspondence locating slot is provided with locating dowel, and the case
Side is provided with vibrating device.
The vibrating device is voice coil motor.
Compared with prior art, due in microbial detection device of the present invention, including optical sampling component and board, institute
State board and be provided with video installing port and culture apparatus mounting table, the culture apparatus is put after culture well in outside insulating box
The culture apparatus mounting table is placed in, testing result can be sent in the display device and be shown by the optical sampling component
Show.
By following description and with reference to accompanying drawing, the present invention will become more fully apparent, and these accompanying drawings are used to explain the present invention
Embodiment.
Brief description of the drawings
Fig. 1 is the schematic diagram of one embodiment of the board of microbial detection device of the present invention.
Fig. 2 is that the culture apparatus of microbial detection device as shown in Figure 1 is positioned over one of optical sampling component implementation
The schematic diagram of example.
Fig. 3 is the schematic diagram of the another embodiment of the board of microbial detection device of the present invention.
Specific embodiment
With reference now to Description of Drawings embodiments of the invention, the element numbers being similar in accompanying drawing represent similar element.Such as
It is upper described, as shown in Fig. 1~2, microbial detection device provided in an embodiment of the present invention, including：
Culture apparatus 1, the culture apparatus 1 includes being transparent casing 10 and case lid 11, is set on the casing 10
Multiple culture tanks 12, and the bottom of the casing 10 is located at embedded fluorescent optical sensor 13 at the lower section of the culture tank 12；
Optical sampling component 2, the optical sampling component 2 includes main body frame 20, LASER Light Source 21, CCD camera 22
And display device (not showing on figure), the upper end of the main body frame 20 be uncovered structure, lower end closed and have round-meshed cylindrical shape
Structure, the LASER Light Source 21 irradiates located at one end of the closing of the main body frame 20 to described uncovered structure one end, described
CCD camera 22 is on the circular hole, and the CCD camera 22 is connected with the display device；
Board 2, the board 2 is provided with display device installing port 30 and culture apparatus mounting table 31, and the culture apparatus is put
The groove 32 offered on platform 31 for placing the culture apparatus 1 is put, the groove 32 is being located at the optical sampling component 2 just
Top, the display device is on the display device installing port 30.
In embodiment as shown in Figure 2, the case lid 11 is just to being provided with boss 14, the case lid 11 at the culture tank 12
Realize being tightly connected with the culture tank 12 by the boss 14.
It should be noted that the multiple culture tanks 12 in the culture apparatus 1 are separate, the culture tank 12
Just the sterile culture environment containing culture medium, and each culture tank 12 be it is separate without mutual infection, need to
The sample solution to be cultivated is injected into the culture tank 12 by syringe from the boss 14, culture tank 12 described in which part
Not sample injection solution, it is as object of reference.
Additionally, the difference of the sample type that the culture apparatus 1 is cultivated as needed, can select to be trained at normal temperatures
Support, it is also possible to which selection is cultivated in insulating box.Treat that the sample in the culture apparatus 1, according to training status, reaches regulation
Incubation time after, be placed on the culture apparatus mounting table 31 on the board 3, be limited in the groove 32
On, be scanned by the optical sampling component 2, and due to the board 3 be with the culture apparatus 1 be lift-off, because
This operating personnel extremely easily can be opened up using the board 3 when using the present invention to the personnel for needing to be demonstrated
Show.
In embodiment as shown in Figure 2, light source optical filter 28 is additionally provided with the LASER Light Source 21.
In embodiment as shown in Figure 2, launching light optical filter 29 and optical system are additionally provided with the CCD camera 22
29a, the light source optical filter 28 and launching light optical filter 29 filter the light of same frequency, and the CCD camera 22 passes through institute
State detection of the optical system 29a realizations to the fluorescent optical sensor 13.
In embodiment as shown in Figure 2, the coarse structure of the inner surface of the main body frame 20 for the benefit of light scattering.
In embodiment as shown in Figure 3, the structure with embodiment as shown in Figure 2 is substantially the same, is a difference in that, institute
State the edge of casing 10 and be provided with locating slot 15, case lid 11 described in the correspondence locating slot 15 is provided with locating dowel 16, and the case lid
11 tops are provided with vibrating device 17.Specifically, the vibrating device 17 is voice coil motor.It is fixed with described by the locating dowel 16
Position groove 15 coordinates, and the case lid 11 stably can be covered on the casing 10, when the shaking device 17 starts, institute
Case lid 11 is stated with the casing 10 as a body vibration, and it is voice coil motor that the vibrating device 17 is selected, using sound
Coil motor produces regular vibration, because voice coil motor is a kind of non-inverting type power set, its positioning precision depends on completely
In feedback and control system, therefore its Oscillation Amplitude is controllable.
Compared with prior art, with reference to Fig. 1~3, due in microbial detection device of the present invention, including optical sampling group
Part 2 and board 3, the board 3 are provided with video installing port 30 and culture apparatus mounting table 31, and the culture apparatus 1 is outside permanent
After being cultivated well in incubator, the culture apparatus mounting table 31 is positioned over, the optical sampling component 2 can send out testing result
Send and shown in the display device.
Above disclosed is only the preferred embodiments of the present invention, can not limit the right of the present invention with this certainly
Scope, therefore the equivalent variations made according to scope of the present invention patent, still belong to the scope that the present invention is covered.
Claims (8)
1. a kind of microbial detection device, it is characterised in that including：
Culture apparatus, the culture apparatus includes transparent casing and case lid, and multiple culture tanks are set on the casing, and described
The bottom of casing is located at culture tank lower section and embeds fluorescent optical sensor；
Optical sampling component, the optical sampling component includes main body frame, LASER Light Source, CCD camera and display device, institute
Main body frame upper end is stated for uncovered structure, lower end closed and have round-meshed columnar structured, the LASER Light Source is located at described
One end of main body frame closing and irradiated to described uncovered structure one end, the CCD camera is on the circular hole and described
CCD camera is connected with the display device；
Board, the board is provided with display device installing port and culture apparatus mounting table, is opened up on the culture apparatus mounting table
There is the groove for placing the culture apparatus, the groove is located at directly over the optical sampling component, the display device
On the display device installing port.
2. microbial detection device as claimed in claim 1, it is characterised in that the groove is being located at the uncovered structure just
Top.
3. microbial detection device as claimed in claim 1 or 2, it is characterised in that the case lid just to the culture tank at
It is provided with boss, the case lid is realized being tightly connected with the culture tank by the boss.
4. microbial detection device as claimed in claim 1, it is characterised in that light source optical filtering is additionally provided with the LASER Light Source
Piece.
5. microbial detection device as claimed in claim 4, it is characterised in that be additionally provided with launching light in the CCD camera
Optical filter and optical system, the light source optical filter and launching light optical filter filter the light of same frequency, the CCD camera
Detection to the fluorescent optical sensor is realized by the optical system.
6. microbial detection device as claimed in claim 1, it is characterised in that the main body frame inner surface for the benefit of light
The coarse structure of scattering.
7. microbial detection device as claimed in claim 2, it is characterised in that the casing edge is provided with locating slot, correspondence
Case lid described in the locating slot is provided with locating dowel, and case top is provided with vibrating device.
8. microbial detection device as claimed in claim 7, it is characterised in that the vibrating device is voice coil motor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710209407.9A CN106918582A (en) | 2017-03-31 | 2017-03-31 | Microbial detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710209407.9A CN106918582A (en) | 2017-03-31 | 2017-03-31 | Microbial detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106918582A true CN106918582A (en) | 2017-07-04 |
Family
ID=59567261
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710209407.9A Pending CN106918582A (en) | 2017-03-31 | 2017-03-31 | Microbial detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106918582A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111897178A (en) * | 2020-08-03 | 2020-11-06 | 佛山科学技术学院 | Device and method for shooting plant growth condition |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1140763A (en) * | 1995-06-27 | 1997-01-22 | 贝克顿迪金森公司 | Method and apparatus for detecting microorganisms |
| CN102313015A (en) * | 2010-07-01 | 2012-01-11 | 中国江南航天工业集团林泉电机厂 | Sealing method and sealing structure for small-sized structures |
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| US20150031119A1 (en) * | 2012-01-27 | 2015-01-29 | Advencis | Device for early detection of micro-organisms |
| CN205152226U (en) * | 2015-08-27 | 2016-04-13 | 北京软隆生物技术有限公司 | Portable microorganism short -term test appearance |
| CN106010960A (en) * | 2016-07-19 | 2016-10-12 | 华测检测认证集团股份有限公司 | Microbiological detection culture dish with reference functions |
| CN106010941A (en) * | 2016-07-19 | 2016-10-12 | 华测检测认证集团股份有限公司 | Culture apparatus for microbiological detection |
| CN205893240U (en) * | 2016-08-08 | 2017-01-18 | 郑州点石生物技术有限公司 | Microorganism culture apparatus |
| CN106481905A (en) * | 2016-10-27 | 2017-03-08 | 安徽江淮汽车集团股份有限公司 | A kind of seal nipple |
| CN206736247U (en) * | 2017-03-31 | 2017-12-12 | 华测检测认证集团股份有限公司 | Microbial detection device |
-
2017
- 2017-03-31 CN CN201710209407.9A patent/CN106918582A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1140763A (en) * | 1995-06-27 | 1997-01-22 | 贝克顿迪金森公司 | Method and apparatus for detecting microorganisms |
| CN102313015A (en) * | 2010-07-01 | 2012-01-11 | 中国江南航天工业集团林泉电机厂 | Sealing method and sealing structure for small-sized structures |
| US20150031119A1 (en) * | 2012-01-27 | 2015-01-29 | Advencis | Device for early detection of micro-organisms |
| CN102816688A (en) * | 2012-09-14 | 2012-12-12 | 江苏嘉语生物医药技术有限公司 | Microorganism culture detection device in oxygen-controllable environment and detection method using same |
| CN205152226U (en) * | 2015-08-27 | 2016-04-13 | 北京软隆生物技术有限公司 | Portable microorganism short -term test appearance |
| CN106010960A (en) * | 2016-07-19 | 2016-10-12 | 华测检测认证集团股份有限公司 | Microbiological detection culture dish with reference functions |
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| CN205893240U (en) * | 2016-08-08 | 2017-01-18 | 郑州点石生物技术有限公司 | Microorganism culture apparatus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111897178A (en) * | 2020-08-03 | 2020-11-06 | 佛山科学技术学院 | Device and method for shooting plant growth condition |
| CN111897178B (en) * | 2020-08-03 | 2022-04-26 | 佛山科学技术学院 | Device and method for shooting plant growth condition |
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