CN106906267A - A kind of method that utilization fermentation method prepares rice bran protein polypeptide from defatted rice bran - Google Patents
A kind of method that utilization fermentation method prepares rice bran protein polypeptide from defatted rice bran Download PDFInfo
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- CN106906267A CN106906267A CN201710249754.4A CN201710249754A CN106906267A CN 106906267 A CN106906267 A CN 106906267A CN 201710249754 A CN201710249754 A CN 201710249754A CN 106906267 A CN106906267 A CN 106906267A
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- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The invention discloses a kind of method that utilization fermentation method prepares rice bran protein polypeptide from defatted rice bran, the method is obtained the rice bran protein polypeptide by preparing the processing steps such as fermentation slurry, microjet separation, centrifugation, enzymolysis.The inventive method is raw material using cheap defatted rice bran, and the rice bran protein polypeptide for preparing is without bitter taste, and dissolubility is high, can be as a kind of excellent food-grade vegetable protein polypeptide human consumption.
Description
Technical field
The present invention relates to technical field of biological fermentation, more particularly, to one kind using fermentation, microjet isolation technics from de-
The method that rice bran protein polypeptide is prepared in fat rice bran.
Background technology
The annual paddy total output of China can produce more than 1,000 ten thousand tons of accessory substance rice at 200,000,000 tons or so, in process
Chaff.Rice bran it is nutritious, wherein protein content is in 12-18%.Rice bran protein is to generally acknowledge quality plant protein, and its is required
Amino acid Compositional balance is reasonable, close to FAO/WHO recommendation patterns；Wherein lysine content is higher, is that other vegetable proteins cannot
Analogy, rice bran protein biological value is very high, and its nutritive value can compare favourably with egg, cow's milk, and digestibility is up to more than 90%；And
Rice bran protein is hypoallergenic albumen, will not produce allergic reaction, therefore, rice bran protein is highly suitable as infant and special
Crowd's nutraceutical.Rice bran protein matter essential amino acid is complete, compared with rice protein, the closer FAO/ of its amino acid composition
WHO recommendation patterns, nutritive value can compare favourably with Chicken Albumin.
Defatted rice bran is the byproduct that rice bran oil is produced using rice bran, and skimming processes make the lipase in rice bran lose work
Property, eliminates the undesirable substances such as fungi, bacterium in rice bran, remains the natural nutrient component of rice bran.Current most domestic
Defatted rice bran is only used as feedstuff and uses, and added value is relatively low, and protein component therein fails to obtain reprocessing and comprehensive profit
With.
Rice bran protein is mainly and is made up of albumin, globulin, alcohol soluble protein and the part of glutelin four, rice bran protein because from
Body contains more disulfide bond, have impact on dissolubility in aqueous, belongs to the relatively low protein of dissolubility.At present to albumen
The modified method of the solubilising of matter has ultrasound, high pressure, acylation and enzyme modification etc., but only for rice bran protein, ultrasonic and high pressure pair
The deliquescent influence of rice bran protein is limited, and acylation belongs to chemical modification, increased the uncertainty of rice bran protein edible safety.It is non-
Enzymolysis of the protease to rice bran protein, the dissolubility influence on albumen is smaller；And albumen fermentoid, the hardship of albumen can be increased
Taste, leverages the mouthfeel and edibility of rice bran protein.
The content of the invention
In view of the above-mentioned problems existing in the prior art, the applicant provides one kind using fermentation method from defatted rice bran
The middle method for preparing rice bran protein polypeptide.The inventive method is raw material, the rice for preparing using cheap defatted rice bran
Chaff polypeptide is without bitter taste, and dissolubility is high, can be as a kind of excellent food-grade vegetable protein polypeptide human consumption.
Technical scheme is as follows：
A kind of method that utilization fermentation method prepares rice bran protein polypeptide from defatted rice bran, the method comprises the following steps：
(1) Jia 1 in defatted rice bran~5 times of water size mixing and by after sterilizing, and feeding carries churned mechanically ventilating fermentation
In tank, 0.5~10% saccharomycete of feed liquid is accessed, ferment 5~24h, fermentation ends obtain fermentation slurry；
(2) step (1) gained fermentation slurry is separated using centrifuge, centrifugal force is (2500-3000) × g, is obtained
Supernatant and precipitation；
(3) the obtained precipitation feeding of step (2) is sized mixing tank, plus 1~3 times of water size mixing, plus alkali lye regulation pH to 8.0~
13.0,40~50 DEG C are heated to, by microjet, the structure that destruction albumen is combined with fiber is obtained reaction solution；
(4) by reaction solution centrifugation obtained in step (3), centrifugal force is (2500-3000) × g, obtains supernatant；
(5) supernatant feeding obtained in step (4) is sized mixing tank, plus 1~3 times of water size mixing, acid liquid regulation pH to 4.0~
4.8, at 40~45 DEG C, mixing speed 50-70r/min reacts 20~60min；
(6) the reaction solution centrifugation that will be processed by step (5), centrifugal force is (2500-3000) × g, obtains rice bran
Albumen.
(7) rice bran protein feeding obtained in step (6) to be sized mixing tank, plus 1~3 times of water size mixing, low whipping speed is 50~
70r/min, temperature is 10~30min of washing under conditions of 30~35 DEG C, is separated using centrifuge, and centrifugal force is (2500-
3000) × g, obtains rice bran protein.
(8) rice bran protein feeding obtained in step (7) is sized mixing tank, plus 1~3 times of water size mixing, and acid liquid regulation pH is 4.2
~4.8, acid protease is added, the rice bran protein polypeptide that 3~8h is digested is digested at 45~60 DEG C.
Saccharomycete described in step (1) is substituted by rhizopus, and addition is the 0.1~3% of feed liquid.
Saccharomycete described in step (1) is respectively feed liquid by saccharomycete and the mixture replacing of rhizopus, addition
0.5%~10%, 0.1~3%.
Supernatant described in step (2) is the degradation product and other solable matters after amylofermentation in defatted rice bran；Institute
State and be precipitated as rice bran protein and fiber.
The flow of microjet described in step (3) is 5~30kg/min, and pressure is 10~40Mpa, the ultimate size of feed liquid
At 25~75 μm.
Step (8) the acidic protein enzyme dosage is 0.5~the 1 ‰ of feed liquid.
Step (8) described acid protease is that yeast produces acid protease, aspergillus product acid protease, aspergillus niger acidity egg
One or more in white enzyme.
The present invention is beneficial to be had technical effect that：
While the present invention is with saccharomycetes to make fermentation defatted rice bran, saccharomycete degradable starch and carbohydrate so that in defatted rice bran
Albumen it is loose with cellulosic structure.Rice bran protein and other non-protein groups are further promoted with the high speed shear effect of microjet
The dissociation for dividing, by the heavy isolated rice bran protein of alkali soluble acid.With acid protease digest rice bran protein, obtain no bitter taste,
Good mouthfeel, dissolubility rice bran protein polypeptide high.
The present invention uses saccharomycete or mould, starch-splitting carbohydrate is carried out, while this two quasi-microorganism secretes some enzymes
Class material, mainly protease, there is certain loose structure to albumen, then further carry out enzyme with acid protease
Solution, promotes the generation of high-quality polypeptide, and fermentation process complements each other with enzymolysis process both effects front and rear, mutually promotees
Enter, be uniformly coordinated again so that finally obtain the polypeptide of high-quality.
Specific embodiment
With reference to embodiment, the present invention is specifically described.
Embodiment 1
A kind of method that utilization fermentation method prepares rice bran protein polypeptide from defatted rice bran, comprises the following steps that：
(1) by defatted rice bran add 1.5 times of water size mixing went forward side by side sterilizing after, feeding carry churned mechanically ventilation fermentation tank
In, access accounts for the saccharomycete of feed liquid 9%, and ferment 24h, and fermentation ends obtain fermentation slurry；
(2) by step (1) gained fermentation slurry separated using centrifuge, centrifugal force be 3000 × g, obtain supernatant and
Precipitation, supernatant is the degradation product and other solable matters after amylofermentation, is precipitated as rice bran protein and fiber；
(3) the obtained precipitation feeding of step (2) is sized mixing tank, plus 1 times of water is sized mixing, plus alkali lye regulation pH to 13.0, feed liquid
To 50 DEG C, by microjet (flow 5kg/min, pressure 12Mpa), the structure that destruction albumen is combined with fiber is obtained anti-for heat exchange
Answer liquid (71 μm of particle diameter)；
(4) reaction solution obtained in step (3) is separated using centrifuge, centrifugal force is 3000 × g, obtains supernatant
To dissolve and separate rice bran protein；
(5) supernatant obtained in step (4) is sent into tank of sizing mixing, plus 1 times of water is sized mixing, acid liquid regulation pH to 4.7,45
DEG C, react 20min under conditions of mixing speed 70r/min；
(6) the reaction solution centrifugation that will be processed by step (5), centrifugal force is 3000 × g, obtains rice bran protein；
(7) rice bran protein feeding obtained in step (6) is sized mixing tank, plus 1 times of water is sized mixing, in 35 DEG C of temperature, mixing speed
10min is washed under conditions of 70r/min, is separated using centrifuge, centrifugal force is 3000 × g, obtains rice bran protein；
(8) rice bran protein obtained in step (7) is sent into tank of sizing mixing, plus 1 times of water is sized mixing, acid liquid regulation pH to 4.6~
4.7,0.5 ‰ acid proteases (Aspergillus niger acidic protease) are added, the rice bran protein polypeptide that 8h is digested is digested at 58 DEG C.
Embodiment 2
A kind of method that utilization fermentation method prepares rice bran protein polypeptide from defatted rice bran, comprises the following steps that：
(1) add 3 times of water to size mixing defatted rice bran to go forward side by side after sterilizing, feeding with churned mechanically ventilation fermentation tank,
Access accounts for the rhizopus of feed liquid 1%, and ferment 12h, and fermentation ends obtain fermentation slurry；
(2) by step (1) gained fermentation slurry separated using centrifuge, centrifugal force be 2800 × g, obtain supernatant and
Precipitation, supernatant is the degradation product and other solable matters after amylofermentation, is precipitated as rice bran protein and fiber；
(3) the obtained precipitation feeding of step (2) is sized mixing tank, plus 2 times of water are sized mixing, plus alkali lye regulation pH to 11.0, feed liquid
To 45 DEG C, by microjet (flow 15kg/min, pressure 25Mpa), the structure that destruction albumen is combined with fiber is obtained anti-for heat exchange
Answer liquid (45 μm of particle diameter)；
(4) reaction solution obtained in step (3) is separated using centrifuge, centrifugal force is 2800 × g, obtains supernatant
To dissolve and separate rice bran protein；
(5) supernatant obtained in step (4) is sent into tank of sizing mixing, plus 1 times of water is sized mixing, acid liquid regulation pH to 4.4,42
DEG C, react 30min under conditions of mixing speed 60r/min；
(6) the reaction solution centrifugation that will be processed by step (5), centrifugal force is 2800 × g, obtains rice bran protein；
(7) rice bran protein feeding obtained in step (6) is sized mixing tank, plus 2 times of water are sized mixing, in 33 DEG C of temperature, mixing speed
20min is washed under conditions of 60r/min, is separated using centrifuge, centrifugal force is 2800 × g, obtains rice bran protein；
(8) rice bran protein obtained in step (7) is sent into tank of sizing mixing, plus 2 times of water are sized mixing, acid liquid regulation pH to 4.3~
4.4,1 ‰ acid proteases (mould acid protease) are added, the rice bran protein polypeptide that 5h is digested is digested at 52 DEG C.
Embodiment 3
A kind of method that utilization fermentation method prepares rice bran protein polypeptide from defatted rice bran, comprises the following steps that：
(1) add 5 times of water to size mixing defatted rice bran to go forward side by side after sterilizing, feeding with churned mechanically ventilation fermentation tank,
Access accounts for the saccharomycete and 3% rhizopus of feed liquid 1%, and ferment 6h, and fermentation ends obtain fermentation slurry；
(2) by step (1) gained fermentation slurry separated using centrifuge, centrifugal force be 2500 × g, obtain supernatant and
Precipitation, supernatant is the degradation product and other solable matters after amylofermentation, is precipitated as rice bran protein and fiber；
(3) the obtained precipitation feeding of step (2) is sized mixing tank, plus 3 times of water are sized mixing, plus alkali lye regulation pH to 8.5, feed liquid is changed
To 40 DEG C, by microjet (flow 30kg/min, pressure 40Mpa), the structure that destruction albumen is combined with fiber is obtained reaction to heat
Liquid (75 μm of particle diameter)；
(4) reaction solution obtained in step (3) is separated using centrifuge, centrifugal force is 2500 × g, obtains supernatant
To dissolve and separate rice bran protein；
(5) supernatant obtained in step (4) is sent into tank of sizing mixing, plus 3 times of water are sized mixing, acid liquid regulation pH to 4.0,40
DEG C, react 20min under conditions of mixing speed 50r/min；
(6) the reaction solution centrifugation that will be processed by step (5), centrifugal force is 2500 × g, obtains rice bran protein；
(7) rice bran protein feeding obtained in step (6) is sized mixing tank, plus 3 times of water are sized mixing, in 30 DEG C of temperature, mixing speed
10min is washed under conditions of 50r/min, is separated using centrifuge, centrifugal force is 2500 × g, obtains rice bran protein；
(8) rice bran protein obtained in step (7) is sent into tank of sizing mixing, plus 3 times of water are sized mixing, acid liquid regulation pH to 4.0~
4.1,0.7 ‰ acid proteases (vegetarian rattan Aspergillus niger acidic protease) are added, the rice bran protein that 3h is digested is digested at 60 DEG C
Polypeptide.Test result is as shown in table 1 to be detected to final products taste and protein content.
Table 1
Note：The fraction of organoleptic quality is higher, and it is better to represent taste, i.e. delicate mouthfeel, does not have bitter taste, does not have delicate flavour, does not have
Saline taste.
As can be seen from Table 1, compared with the rice bran protein polypeptide of certain brand, Fed Protein Powder of Pea Insteal prepared by three embodiments,
Quality is higher than reference substance, and substantially without bitter taste, dissolubility is high, and without delicate flavour, without saline taste, protein content is more than 80%.It is especially real
The rice bran protein polypeptide that example three is prepared is applied, preferably, protein content highest, dissolubility is high, without delicate flavour, without saline taste, mouth for mouthfeel
Sense is fine and smooth, and evaluation result is preferable.The inventive method can prepare good mouthfeel, superior in quality rice bran protein polypeptide.
Claims (7)
1. a kind of method that utilization fermentation method prepares rice bran protein polypeptide from defatted rice bran, it is characterised in that the method is included such as
Lower step：
(1) Jia 1 in defatted rice bran~5 times of water size mixing and by after sterilizing, feeding with churned mechanically ventilation fermentation tank,
0.5~10% saccharomycete of feed liquid is accessed, ferment 5~24h, fermentation ends obtain fermentation slurry；
(2) step (1) gained fermentation slurry is separated using centrifuge, centrifugal force is (2500-3000) × g, obtains supernatant
Liquid and precipitation；
(3) the obtained precipitation feeding of step (2) is sized mixing tank, plus 1~3 times of water size mixing, plus alkali lye adjusts pH to 8.0~13.0,
40~50 DEG C are heated to, by microjet, the structure that destruction albumen is combined with fiber is obtained reaction solution；
(4) by reaction solution centrifugation obtained in step (3), centrifugal force is (2500-3000) × g, obtains supernatant；
(5) supernatant feeding obtained in step (4) is sized mixing tank, plus 1~3 times of water size mixing, and acid liquid adjusts pH to 4.0~4.8,
At 40~45 DEG C, mixing speed 50-70r/min reacts 20~60min；
(6) the reaction solution centrifugation that will be processed by step (5), centrifugal force is (2500-3000) × g, obtains rice bran protein.
(7) rice bran protein feeding obtained in step (6) is sized mixing tank, plus 1~3 times of water size mixing, and low whipping speed is 50~70r/
Min, temperature be 30~35 DEG C under conditions of wash 10~30min, using centrifuge separate, centrifugal force be (2500-3000) ×
G, obtains rice bran protein.
(8) rice bran protein feeding obtained in step (7) being sized mixing tank, plus 1~3 times of water size mixing, acid liquid regulation pH is 4.2~
4.8, acid protease is added, the rice bran protein polypeptide that 3~8h is digested is digested at 45~60 DEG C.
2. method according to claim 1, it is characterised in that saccharomycete described in step (1) is substituted by rhizopus, addition
Measure is the 0.1~3% of feed liquid.
3. method according to claim 1, it is characterised in that saccharomycete described in step (1) is by saccharomycete and rhizopus
Mixture replacing, addition is respectively the 0.5%~10% of feed liquid, 0.1~3%.
4. method according to claim 1, it is characterised in that supernatant described in step (2) is starch hair in defatted rice bran
Degradation product and other solable matters after ferment；It is described to be precipitated as rice bran protein and fiber.
5. method according to claim 1, it is characterised in that the flow of microjet described in step (3) is 5~30kg/
Min, pressure is 10~40Mpa, and the ultimate size of feed liquid is at 25~75 μm.
6. method according to claim 1, it is characterised in that step (8) the acidic protein enzyme dosage is the 0.5 of feed liquid
~1 ‰.
7. method according to claim 1, it is characterised in that step (8) described acid protease is that yeast produces acidic protein
Enzyme, aspergillus produce one or more in acid protease, Aspergillus niger acidic protease.
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Citations (5)
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| CN102870953A (en) * | 2012-09-06 | 2013-01-16 | 广东省农业科学院农业生物技术研究所 | Processing method for solubilizing and modifying rice bran protein |
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| CN103478400B (en) * | 2013-09-11 | 2015-01-28 | 无锡群硕谷唐生物科技有限公司 | High-purity rice bran protein and rice bran fibers and preparation method thereof |
| US20160278402A1 (en) * | 2014-01-07 | 2016-09-29 | Ian Mackay | Process for Producing Protein Concentrate and A Cellulosic Residue Material From Defatted Rice Bran |
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2017
- 2017-04-17 CN CN201710249754.4A patent/CN106906267A/en active Pending
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| CN102870953A (en) * | 2012-09-06 | 2013-01-16 | 广东省农业科学院农业生物技术研究所 | Processing method for solubilizing and modifying rice bran protein |
| CN103478400B (en) * | 2013-09-11 | 2015-01-28 | 无锡群硕谷唐生物科技有限公司 | High-purity rice bran protein and rice bran fibers and preparation method thereof |
| CN103833786A (en) * | 2013-11-30 | 2014-06-04 | 江南大学 | High-efficiency extraction method of nutritional and active substances of rice bran |
| US20160278402A1 (en) * | 2014-01-07 | 2016-09-29 | Ian Mackay | Process for Producing Protein Concentrate and A Cellulosic Residue Material From Defatted Rice Bran |
| CN106520884A (en) * | 2017-01-09 | 2017-03-22 | 河南工业大学 | Preparation method of selenium-rich protein active peptide |
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