CN103478400B - High-purity rice bran protein and rice bran fibers and preparation method thereof - Google Patents
High-purity rice bran protein and rice bran fibers and preparation method thereof Download PDFInfo
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Abstract
The invention relates to high-purity rice bran protein and rice bran fibers, and further relates to a preparation method of the high-purity rice bran protein and the rice bran fibers. The preparation method includes the steps of raw material pretreatment, first size mixing and enzyme reaction, second size mixing and enzyme reaction, separate washing, drying and the like. According to the method, through superfine grinding and amylase and cellulose hydrolyzing, components of defatted rice bran are effectively separated, a whirlcone is utilized to conduct the washing treatment, and therefore separate washing efficiency is greatly improved. Compared with spray drying, flash drying equipment is utilized, investment is saved by about 40%, operating cost is saved by about 35%, in rice bran protein products, the protein content is over 80%, the ash content is below 3%, the fat content is below 0.5%, the protein extraction ratio is over 75%, and in rice bran fiber products, the fiber content is over 70%, the ash content is below 4%, and the fat content is below 1%. The high-purity rice bran protein and the rice bran fibers all can be widely applied to the field of health food, nutritious food and the like, and additional value of rice deep processing is greatly improved.
Description
[technical field]
The invention belongs to rice deep process technology field.More specifically, the present invention relates to high-purity rice bran protein and rice bran-fiber, also relate to the preparation method of high-purity rice bran protein and rice bran-fiber.
[background technology]
Rice bran is that compared with highed milled rice, rice bran and rice embryo contain the nutrients such as higher albumen, fat, dietary fiber, vitamin and mineral matter by the mixture grinding lower cortex and a small amount of rice embryo and crack rice in brown rice pearling process.The a research report of United States Department of Agriculture claims, and in paddy, the nutrient of 64% concentrates in rice bran.Due to the effect of lipase contained in paddy, if process not in time, fat in the rice bran produced after husk rice can lose by rapid oxidation oyster, therefore most of meters of factories all can carry out stabilizing rice bran by extrusion rapidly after husk rice, and then extract rice bran oil as raw material, although fat and liposoluble substance are removed mostly in the defatted rice bran after oil expression, but still remain most nutrition in rice bran, albumen, dietary fiber, carbohydrate, various minerals and vitamins, and extract the defatted rice bran after rice bran oil at present often all as feed process, this wastes greatly high-quality protein resource.
Rice bran protein is universally acknowledged quality plant albumen, research for many years shows, protein in rice bran is a kind of hypoallergenic albumen, its amino acid composition is close to the recommendation pattern of FAO/WHO, its nutritive value is far above the vegetable protein such as corn, wheat, can match in excellence or beauty with the high-quality such as egg, milk animal protein, be a kind of novel plant albumen of great exploitation potential for its.According to rough reckoning, China can obtain about 2,000 ten thousand tons, rice bran every year, is a kind of renewable resource had a large capacity and a wide range, if protein wherein all can be extracted, can obtain the quality plant albumen of about 2,000,000 tons every year.Therefore, from rice bran, seek new protein resource, increase gradually for alleviation China population, the present situation that protein resource is day by day in short supply has important practical significance.
Rice bran protein is divided into albumin, globulin, glutelin and alcohol soluble protein, four kinds of protein ratios are roughly 37: 36: 22: 5, namely the albumen dissolving in weak brine roughly accounts for about 80%, but it is expanded owing to have passed through high temperature extrusion, again after squeezing or solvent high-temp extracting, albumen substantially all sex change, so very low by the yield of traditional alkali extraction-acid precipitation extraction rice bran protein, and when concentration of lye is too high, product special flavour can be affected and color and luster makes the color of protein extract deeply dark, also adverse effect may be produced simultaneously, change the nutritive peculiarity of protein.
Extracting Rice Bran Protein by Enzymatic Process reaction condition is gentle, and can more retaining protein nutritive values, therefore, in recent years, adopts the research of Enzymatic Extraction albumen quite active both at home and abroad.At present, for extracting enzyme mainly protease, carbohydrase and the phytase etc. of rice bran protein.Rice bran protein molecular degradation is mainly solubility by their mechanism of action, or by its from hemicellulose, extract after depolymerization in the compound that phytic acid etc. are formed.Carbohydrase can be degraded histocyte wall, is conducive to the extraction of protein in rice bran, particularly protein and phytic acid, hemicellulose etc. are cross-linked to form compound, makes protein be easy to extract after adopting phytase, zytase to remove cross-linked state.1997, Ansharullah proposed to adopt carbohydrase, destroyed the recovery rate that plant cell wall improves vegetable protein.1999, this method was applied in rice bran by Hettiararachchy, adopted hemicellulase and phytase conbined usage, and extract rice bran protein, protein extracting ratio can reach 92%.Domestic Wang Li etc. adopt protease, carbohydrase to extract separating protein from rice bran respectively, and its protein extracting ratio is about 60%.In sum, although Extracting Rice Bran Protein by Enzymatic Process increases on the recovery rate of albumen, because cellulose, aging macromolecular amylin and ash grade the impact of impurity, the purity of gained rice bran protein is not high.
In recent decades, it is found that the generation of numerous disease is relevant with the minimizing increasingly of dietary fiber intake with development.These diseases comprise: constipation, diverticulosis, colorectal cancer, anal fissure, typhlitis, obesity, coronary heart disease, duodenal ulcer, breast cancer and calculus.Although the effect of dietary fiber is not all confirmed in all diseases, confirmed by scientific research in promotion gastrointestinal health, reduction cardiovascular disease incidence rate, the function of preventing and treating in obesity, reduction onset diabetes rate four.Maize peel, rice bran, wheat bran and the natural dietary fiber such as oat bran, sugar beet fiber have been proved has reduction blood fat, alleviates hyperlipidemia and the ability with development occurs.But paying close attention in the research of extracting rice bran protein at present, rice bran-fiber in defatted rice bran and other functional materials do not obtain enough attention, as with after Enzymatic Extraction albumen, due to the reasons such as the left and right condition of enzyme preparation will be met, extract in the residue after albumen and often have higher ash content and carbohydrate etc., even after extracting albumen with protease hydrolytic, the smell of residue and local flavor are all very poor, are difficult to continue to use as food.
In order to solve the disadvantage of prior art, should obtain the rice bran protein of higher degree, high extraction, also synchronously will obtain rice bran-fiber and other water-insoluble functional materials, the present inventor is through test of many times, and research completes the present invention.
[summary of the invention]
[technical problem that will solve]
The object of this invention is to provide high-purity rice bran protein and rice bran-fiber.
Another object of the present invention is to provide the preparation method of described high-purity rice bran protein and rice bran-fiber.
[technical scheme]
The present invention is achieved through the following technical solutions.
The present invention relates to the preparation method of a kind of high-purity rice bran protein and rice bran-fiber.
The step of described method is as follows:
A, pretreatment of raw material
Adopt method for sieving, by protein content by weight 16% ~ 20% defatted rice bran raw material in the tangible foreign matter that exists remove; Then, the defatted rice bran raw material of screening uses disintegrating apparatus to pulverize, and obtains defatted rice bran powder;
B, first time size mixing and enzyme reaction
Add water in the defatted rice bran powder that steps A obtains and size mixing, obtain the feed liquid that defatted rice bran powder concentration is by weight 20% ~ 28%, re-use 8 ~ 12%NaOH aqueous solution by weight and the pH value of described feed liquid is adjusted to 6.0 ~ 6.5, then add in described feed liquid with the mesophilicα-diastase of defatted rice bran grain weight gauge 0.6 ~ 0.9%, the enzyme feed liquid that contains of mixing is heated to 65 ~ 70 DEG C again, and carry out enzyme digestion reaction 0.5 ~ 1.0h at this temperature, terminate the temperature of its enzyme digestion reaction liquid to be reduced to 45 ~ 50 DEG C at enzyme digestion reaction, obtain first time enzymolysis slurries;
C, second time are sized mixing and enzyme reaction
Use 0.8 ~ 1.2mol/LHCl aqueous solution to be obtained by step B first time enzymolysis slurries pH value be adjusted to 4.8 ~ 5.2, then add in described enzymolysis slurries with defatted rice bran grain weight gauge 0.5% ~ 0.8% cellulase, at temperature 48 ~ 52 DEG C, carry out enzyme digestion reaction 3.0 ~ 4.0h after mixing, obtain second time enzymolysis slurries;
D, separating, washing
The second time enzymolysis slurries obtained by step C are washed by cyclone under the following conditions: feed pressure 0.7 ~ 0.8MPa, underflow pressure 0.3 ~ 0.4MPa, feed rate 5.5 ~ 6.0t/h, leaching requirement 5.5 ~ 6.0t/h; Then obtained eddy flow gently filters respectively through flame filter press with heavy phase, obtains rice bran protein filter cake and rice bran-fiber filter cake, and they use respectively again washs with described filter cake weighing scale 2 ~ 4 times of water;
E, drying
The rice bran protein filter cake that step D obtains and rice bran-fiber filter cake, flash drying equipment is adopted to carry out drying under the condition of EAT 160 ~ 165 DEG C and leaving air temp 60 ~ 65 DEG C, until its water content is with dry weight basis less than 4%, so obtain described rice bran protein and rice bran-fiber.
A preferred embodiment of the invention, in step, uses micronizer that defatted rice bran raw meal is broken to grain fineness 120 ~ 150 order.
According to another kind of preferred embodiment of the present invention, in stepb, the concentration of defatted rice bran powder liquid is by weight 23% ~ 25%.
According to another kind of preferred embodiment of the present invention, in stepb, the addition of described mesophilicα-diastase is with defatted rice bran grain weight gauge 0.68 ~ 0.82%.
According to another kind of preferred embodiment of the present invention, in stepb, defatted rice bran powder liquid and mesophilicα-diastase carry out enzyme digestion reaction 0.6 ~ 0.8h at temperature 66 ~ 68 DEG C.
According to another kind of preferred embodiment of the present invention, in stepb, use plate type heat exchanger that defatted rice bran powder liquid and mesophilicα-diastase mixture are heated to 66 ~ 68 DEG C, after enzyme digestion reaction terminates, the temperature of its enzyme digestion reaction liquid is reduced to 46 ~ 48 DEG C.
According to another kind of preferred embodiment of the present invention, in step C, second time enzyme digestion reaction carries out reaction 3.4 ~ 3.6h at temperature 49 ~ 50 DEG C.
According to another kind of preferred embodiment of the present invention, in step C, the addition of described cellulase is with defatted rice bran grain weight gauge 0.6% ~ 0.7%.
According to another kind of preferred embodiment of the present invention, in step D, second time enzymolysis slurries use cyclone to wash under the condition of feed pressure 0.74 ~ 0.76MPa, underflow pressure 0.34 ~ 0.36MPa, feed rate 5.6 ~ 5.8t/h, leaching requirement 5.6 ~ 5.8t/h.
The invention still further relates to the product adopting described preparation method to obtain.With described rice bran protein total weight, the protein content of described rice bran protein is more than 80%, content of ashes less than 3% and fat content less than 0.5%; The recovery rate of described rice bran protein is more than 75%; With described rice bran-fiber total weight, the content of ashes of described rice bran-fiber below 4%, fat content less than 1%.
In more detail the present invention will be described below.
The present invention relates to the preparation method of a kind of high-purity rice bran protein and rice bran-fiber.
The step of described method is as follows:
A, pretreatment of raw material
Adopt method for sieving, by protein content by weight 16% ~ 20% defatted rice bran raw material in the tangible foreign matter that exists remove; Then, the defatted rice bran raw material of screening uses disintegrating apparatus to pulverize, and obtains defatted rice bran powder;
To extract the defatted rice bran after rice bran oil for raw material, method for sieving or manual selection method is adopted to remove large caking and tangible foreign matter, such as stone, plant residue etc.The protein content by weight 16% ~ 20% of the defatted rice bran raw material that the present invention uses, mainly ash content, cellulose and the macromolecular amylin of other component in described raw material.
The defatted rice bran that the present invention uses is product sold in the market, such as Jiangxi peasants who dig gold meter Ye Group Co., Ltd, Jiangxi Province and conjunction limited industrial company, Baofeng, Yutai County grease Co., Ltd product sold.
In the present invention, described defatted rice bran raw material uses micronizer to be crushed to grain fineness 120 ~ 150 order.
In this step, the object pulverized is the eucaryotic cell structure in defatted rice bran is damaged, make to be wrapped in macromolecular amylin wherein, fat, the ash impurity that grades discharges, thus be conducive to removing these impurity, also be conducive to rice bran protein release simultaneously, be convenient to extract rice bran protein, obtain rice bran-fiber.
In the present invention, described pulverizing makes the grain fineness of rice residue protein reach 120 ~ 150 orders, if grain fineness is greater than 120 orders, then the not good content that causes of eucaryotic cell structure fragmentation cannot discharge; If when grain fineness is less than 150 order, then particle is too little when causing separating, washing, and gravitational difference, apart from little, affect protein purification and recovery, and disintegrating apparatus investment greatly, energy consumption is large; Therefore, described grain fineness is 120 ~ 150 orders is suitable, preferably 120 ~ 140 orders.
The Ultra-Micro Grinding Equipment used in the present invention is product sold, such as Jiangyin City Wei Dayaohua Machinery Manufacturing Co., Ltd., Hai Ke powder equipment Manufacturing Co., Ltd of Jiangyin City, Longchang Mechanical Mfg. Co., Ltd., Jiangyin's product sold in the market.
B, first time size mixing and enzyme reaction
Add water in the defatted rice bran powder that steps A obtains and size mixing, obtain the feed liquid that defatted rice bran powder concentration is by weight 20% ~ 28%, re-use 8 ~ 12%NaOH aqueous solution by weight and the pH value of described feed liquid is adjusted to 6.0 ~ 6.5, then add in described feed liquid with the mesophilicα-diastase of defatted rice bran grain weight gauge 0.6 ~ 0.9%, the enzyme feed liquid that contains of mixing is heated to 65 ~ 70 DEG C again, and carry out enzyme digestion reaction 0.5 ~ 1.0h at this temperature, terminate the temperature of its enzyme digestion reaction liquid to be reduced to 45 ~ 50 DEG C at enzyme digestion reaction, obtain first time enzymolysis slurries;
In the present invention, the defatted rice bran powder obtained toward steps A adds water and sizes mixing, and makes defatted rice bran powder become feed liquid containing defatted rice bran powder, so that the mass transport of subsequent processing steps, reaction and separating, washing.The concentration of described feed liquid is with described feed liquid total weight for 20% ~ 28%, if this concentration is less than 20%, then the low reaction effect that causes of reaction substrate concentration is poor, and material treating capacity is large; If this concentration higher than 28% time, then material viscosity is large, is unfavorable for conveying and separating, washing; Therefore, the concentration of described feed liquid is 20% ~ 28% is suitable, preferably 23% ~ 25%, more preferably 23.5% ~ 24.5%.
In the present invention, the raw material used can enter rice bran by entrainment portions rice starch in rice milling process, the gelatinization under extruding and high temperature action of these starch, the viscosity of sludge of defatted rice bran is increased, be unfavorable for filtering, and the proportion of rice starch granule is close with rice bran protein, general separation means is not easy both to separate, and therefore can make starch and Protein Separation by amylase liquefaction.The mesophilicα-diastase that the present invention uses is the starch liquefacation enzyme that bacillus amyloliquefaciens obtains through fermenting, refining, and it can shear gelatinized starch or macromolecular amylin, makes them liquefy.
In the present invention, the operative temperature of mesophilicα-diastase is 40 ~ 70 DEG C, and enzyme work with temperature improve and improve, from microorganism angle and maximize enzyme effect angle consider, it is suitable that described feed liquid is heated to 65 ~ 70 DEG C.
Under the enzymatic hydrolysis condition of pH6.0 ~ 6.5 with temperature 65 ~ 70 DEG C, if the addition of mesophilicα-diastase is less than 0.6%, then enzyme digestion reaction overlong time, enzymolysis is not thorough, filtration difficulty; If the addition of mesophilicα-diastase is greater than 0.9%, then the reaction time is too short, unit operations can not be completed in the industrial production within the short time like this, fail at utmost to play the effect of enzyme preparation, cost increases, and therefore, the addition of mesophilicα-diastase is 0.6 ~ 0.9% is appropriate, preferably 0.68 ~ 0.82%, more preferably 0.72 ~ 0.78%.
The mesophilicα-diastase that the present invention uses is product sold in the market, such as Novi's letter (China) Bioisystech Co., Ltd, Jie Neng section (China) bioengineering Co., Ltd, the grand mcroorganism Engineering Co., Ltd in Shandong product sold.
In the present invention, defatted rice bran powder liquid and mesophilicα-diastase carry out enzyme digestion reaction 0.5 ~ 1.0h at temperature 65 ~ 70 DEG C.In the present invention, if this reaction time is less than 0.5h, then liquefy not thorough; If this reaction time is longer than 1.0h, then amylase also can not work again.
Preferably, defatted rice bran powder liquid and mesophilicα-diastase carry out enzyme digestion reaction 0.6 ~ 0.8h at temperature 66 ~ 68 DEG C.
After described enzyme digestion reaction terminates, the temperature of its enzyme digestion reaction liquid is reduced to 45 ~ 50 DEG C.
In this step, heating is all use plate type heat exchanger to realize with cooling.
Preferably, in this step, use plate type heat exchanger that defatted rice bran powder liquid and mesophilicα-diastase mixture are heated to 66 ~ 68 DEG C, after enzyme digestion reaction terminates, the temperature of its enzyme digestion reaction liquid is reduced to 46 ~ 48 DEG C.
Described plate type heat exchanger is extensively product sold, such as Shanghai Nanhua Transducer Manufacture Co., Ltd., Tianjin General Heat Exchanging Equipment Factory, Changzhou Sheng Ge heat exchanger Co., Ltd product sold in the market.
C, second time are sized mixing and enzyme reaction
Use 0.8 ~ 1.2mol/LHCl aqueous solution to be obtained by step B first time enzymolysis slurries pH value be adjusted to 4.8 ~ 5.2, then add in described enzymolysis slurries with defatted rice bran grain weight gauge 0.5% ~ 0.8% cellulase, at temperature 48 ~ 52 DEG C, carry out enzyme digestion reaction 3.0 ~ 4.0h after mixing, obtain second time enzymolysis slurries.
In the present invention, use cellulase to break the eucaryotic cell structure of rice bran, the structure that cellulose is maintained is damaged, and content is wherein released, and comprises albumen and ash and to grade impurity, be convenient to later separation purifying.
The cellulase that the present invention uses is a kind of by fungal metabolite generation, the cellulolytic multiply anchor-pile of energy.The cellulase that the present invention uses is product sold in the market, such as Novi's letter (China) Bioisystech Co., Ltd, Jie Neng section (China) bioengineering Co., Ltd, the grand mcroorganism Engineering Co., Ltd in Shandong product sold.
In the present invention, the addition of described cellulase is with defatted rice bran grain weight gauge 0.5% ~ 0.8%, preferably 0.6% ~ 0.7%, more preferably 0.62% ~ 0.65%.
In the present invention, enzymolysis slurries use cellulase to carry out second time enzyme digestion reaction 3 ~ 4h under the condition of pH4.8 ~ 5.2 with temperature 48 ~ 52 DEG C for the first time.
The time of described enzyme digestion reaction is less than 3h, then fibre structure is for opening, and protein delivery is incomplete, affects protein extracting ratio; If the time of described enzyme digestion reaction is longer than 4h, then cellulose may resolve into more small fragment, therefore reduces with the difference of protein molecular proportion, is unfavorable for like this being separated, and therefore reaction time 3 ~ 4h is suitable.
Preferably, second time enzyme digestion reaction carries out reaction 3.4 ~ 3.6h at temperature 49 ~ 50 DEG C.
D, separating, washing
The second time enzymolysis slurries obtained by step C are washed by cyclone under the following conditions: feed pressure 0.7 ~ 0.8MPa, underflow pressure 0.3 ~ 0.4MPa, feed rate 5.5 ~ 6.0t/h, leaching requirement 5.5 ~ 6.0t/h; Then obtained eddy flow gently filters respectively through flame filter press with heavy phase, obtains rice bran protein filter cake and rice bran-fiber filter cake, and they use respectively again washs with described filter cake weighing scale 2 ~ 4 times of water.
In the present invention, the second time enzymolysis slurries that step C obtains use cyclone separating, washing, material mainly ash content, the small dextrins etc. of solubility in the slurries obtained after each step process above, insoluble material is albumen, cellulose mainly, therefore, as long as the rice bran protein that the cellulose making proportion heavier is lighter with proportion is separated, the object of purifying rice bran protein just tentatively can be reached.
Cyclone is a kind of classifying equipoment being separated non-homogeneous phase mixture.It may be used for liquid clarification, solid phase particles washing, liquid degassing and desanding, solid phase particles classification and classification and two kinds of non-mutually soluble liquids the several work such as to be separated.In the present invention, second time enzymolysis slurries enter cyclone, and under the cyclone pressure effect of continuous flow upstream, the heavier cellulose of proportion is discharged from underflow opening, the lighter rice bran protein of proportion is then discharged from overfall, achieves being separated of cellulose and rice bran protein like this.Described feed pressure is 0.7 ~ 0.8MPa, as feed pressure is less than 0.7MPa, then act on albumen to be effectively separated to make both with cellulosic insufficient pressure, if feed pressure is greater than 0.8MPa, then the protein concentration of overfall discharge is lower, mean that the impurity content of solubility is high, be unfavorable for improving rice bran protein purity; Described underflow pressure is 0.3 ~ 0.4MPa, if pressure is less than 0.3MPa, then has part rice bran protein and also discharges from underflow opening, be unfavorable for improving protein recovery; If underflow pressure is greater than 0.4MPa, then effluent concentration reduces, and is unfavorable for improving purity of protein; Described feed rate is 5.5 ~ 6t/h, and described leaching requirement is 5.5 ~ 6t/h, and the parameter both this links in feed pressure and underflow pressure, and simultaneously inlet amount is large and leaching requirement is little, then albumen is many containing soluble impurity; If inlet amount is little and leaching requirement large, then albumen and fiber separation weak effect.
The cyclone that the present invention uses is product sold, such as Yuanan, Tianzhang City Machinery Co., Ltd., Jiangsu Bang Li biochemical equipment Co., Ltd, Weihai Haiwang Hydrocyclone Co., Ltd., Beijing Huayu Engineering Co., Ltd. of Zh's product sold in the market.
Preferably, second time enzymolysis slurries use cyclone to wash under the condition of feed pressure 0.74 ~ 0.76MPa, underflow pressure 0.34 ~ 0.36MPa, feed rate 5.6 ~ 5.8t/h, leaching requirement 5.6 ~ 5.8t/h.
This step uses cyclone to obtain the light phase of eddy flow and heavy phase.They are respectively through plate-frame filtering, obtain rice bran protein filter cake and rice bran-fiber filter cake, then use respectively and wash with described filter cake weighing scale 2 ~ 4 times of water.Eddy flow light mutually in the main impurity containing insoluble rice bran protein and solubility, filtered by plate and frame filter press and can remove most of impurity, filter the filter cake available water obtained and rinse, impurity can be removed further, obtain highly purified rice bran protein; Main containing insoluble rice bran-fiber and other insoluble functional materials in eddy flow heavy phase, after plate and frame filter press filtration, washing, obtain the rice bran-fiber of higher degree.
The plate and frame filter press that the present invention uses is product sold, such as Hangzhou Guo Rui filter press Co., Ltd, Hangzhou Kun Yuan filtration machinery Co., Ltd, Hangzhou Xingyuan Filter Technology Co., Ltd.'s product sold in the market.
E, drying
The rice bran protein filter cake that step D obtains and rice bran-fiber filter cake, flash drying equipment is adopted to carry out drying under the condition of EAT 160 ~ 165 DEG C and leaving air temp 60 ~ 65 DEG C, until its water content is with dry weight basis less than 4%, so obtain described rice bran protein and rice bran-fiber.
The flash drying machine that the present invention uses is product sold, such as Jiangsu Xianfeng Drying Engineering Co., Ltd., Changzhou Lemar Drying Engineering Co., Ltd., Co., Ltd of Changzhou City No.2 Drying Equipment Factory product sold in the market.
The invention still further relates to the product adopting described preparation method to obtain.With described rice bran protein total weight, the protein content of described rice bran protein is more than 80%, content of ashes less than 3% and fat content less than 0.5%; The recovery rate of described rice bran protein is more than 75%; With described rice bran-fiber total weight, the content of ashes of described rice bran-fiber is less than 4%, fat content less than 1%.
In the present invention,
The protein content of rice bran protein and rice bran-fiber adopts GB5009.5-2010 method to measure.
The ash content of rice bran protein and rice bran-fiber adopts GB5009.4-2010 method to measure.
The fat of rice bran protein and rice bran-fiber adopts GB/T5009.6-2003 method to measure.
The total reducing sugar of rice bran protein and rice bran-fiber adopts GB/T5009.7-2008 method to measure.
The crude fiber content of defatted rice bran and rice bran-fiber adopts GB/T5515-2008 method to measure.
According to the following equation by defatted rice bran material quantity with prepare rice bran protein gauge and calculate protein extracting ratio:
Protein recovery=rice bran protein quality × protein content ÷ (defatted rice bran material quantity × protein content)
Method of the present invention has following feature:
I, compared with prior art, the present invention is to extract the defatted rice bran after rice bran oil for raw material, first by ultramicro grinding broken cell structure, then with amylase, the starch in raw material and macromolecular amylin are liquefied, both reduced the viscosity of feed liquid, and made again this part impurity be easy to be separated with insoluble rice bran protein; Reprocess fibre element enzyme decomposition of cellulose, to destroy package structure further, makes albumen and soluble impurity be discharged, and the effect that is interconnected in raw material between each component is destroyed, and is beneficial to separation and purification.
II, the present invention carries out continuous flow upstream separating, washing by cyclone to feed liquid, by insoluble albumen and cellulosic difference in specific gravity distance, two kinds of solid phase particles are separated and washing, the lighter rice bran protein of proportion is from overfall discharging, the heavier cellulose of proportion is from underflow opening discharging, by the adjustment of parameter, the albumen of overfall discharging can be made as far as possible high, thus improve the rate of recovery of albumen, and the concentration of protein solution is as far as possible low, wherein contained soluble impurity is just low, thus the purity of rice bran protein can be improved, light phase and heavy phase are respectively through after filtration washing, just can obtain rice bran protein and the rice bran-fiber of higher degree.
III, compared with mostly adopting spraying dry with prior art, the present invention adopts flash drying equipment dried rice albumen, and input concentration brings up to 45% ~ 55% from 16% ~ 20%, greatly reduces treating capacity, thus has saved equipment investment cost; The drying efficiency of flash drying improves greatly, greatly can save energy consumption; The baking temperature of flash drying is low, avoids rice protein to crust in dry run caking, farthest can retain the good water absorption and swelling performance that rice protein has in process engineering.
[beneficial effect]
The invention has the beneficial effects as follows: the present invention is to extract the defatted rice bran after rice bran oil for raw material, for raw material, first pass through ultramicro grinding, the enzymolysis of amylase and cellulase, make to be separated from each other between each component in defatted rice bran, then by the classification adverse current cleaning function of cyclone, make wherein insoluble albumen and cellulose respectively from overfall and the underflow opening discharging of cyclone, the separation of both realizations, substantially increase the efficiency of separating, washing, and saving slurry, wash through dewaterer again, make the magazins' layout of insoluble component and solubility, eventually pass flash drying and obtain highly purified rice bran protein and rice bran-fiber, adopt flash drying compared with spraying dry, equipment investment saves about 40%, operating cost about saves 35%.The dry protein content adopting the inventive method to produce the rice bran protein obtained is more than 80%, content of ashes less than 3%, fat content less than 0.5%, protein extracting ratio more than 75%.The ash content adopting the inventive method to produce the rice bran-fiber obtained is less than 4%, fat content less than 1%.According to relevant national standard regulation, adopt the inventive method to produce the two kinds of products obtained and all can be widely used in the field such as health food, nutraceutical, greatly improve the added value of rice deep processing.
[detailed description of the invention]
The present invention can be understood better by following embodiment.
Embodiment 1: the preparation of high-purity rice bran protein and rice bran-fiber
The implementation step of this embodiment is as follows:
A, pretreatment of raw material
Adopt method for sieving, the tangible foreign matter existed in the defatted rice bran raw material (with defatted rice bran raw material total weight, protein content 18.1%, ash content 13.9%, fat 0.95%, total reducing sugar 30.6%, crude fibre 25.7%) 2000kg Jiangxi peasants who dig gold meter Ye Co., Ltd sold is removed; Then, the defatted rice bran raw material of screening uses the disintegrating apparatus sold by Wei Dayaohua Machinery Manufacturing Co., Ltd. of Jiangyin City to be crushed to grain fineness 120 order, obtains defatted rice bran powder;
B, first time size mixing and enzyme reaction
Add water in the defatted rice bran powder that steps A obtains and size mixing, obtain the feed liquid that defatted rice bran powder concentration is by weight 20%, re-use the 10%NaOH aqueous solution by weight and the pH value of described feed liquid is adjusted to 6.2, then in described feed liquid, add the mesophilicα-diastase sold with letter (China) Bioisystech Co., Ltd of the Novi of defatted rice bran grain weight gauge 0.6%, the re-using the plate type heat exchanger sold by Shanghai Nanhua Transducer Manufacture Co., Ltd. be heated to 65 DEG C containing enzyme feed liquid of mixing, and carry out enzyme digestion reaction 0.5h at this temperature, after enzyme digestion reaction terminates, use above-mentioned plate type heat exchanger that the temperature of its enzyme digestion reaction liquid is reduced to 45 DEG C, obtain first time enzymolysis slurries,
C, second time are sized mixing and enzyme reaction
Use the 10mol/LHCl aqueous solution to be obtained by step B first time enzymolysis slurries pH value be adjusted to 4.8, then add in described enzymolysis slurries and believe by Novi the cellulase that (China) Bioisystech Co., Ltd sells with defatted rice bran grain weight gauge 0.5%, at temperature 48 DEG C, carry out enzyme digestion reaction 3.0h after mixing, obtain second time enzymolysis slurries;
D, separating, washing
The second time enzymolysis slurries obtained by step C use the cyclone sold by Yuanan, Tianzhang City Machinery Co., Ltd. to wash under the following conditions: feed pressure 0.7MPa, underflow pressure 0.3MPa, feed rate 5.5t/h, leaching requirement 5.5t/h; Then obtained eddy flow gently filters respectively through the flame filter press sold by Hangzhou Guo Rui filter press Co., Ltd with heavy phase, obtain rice bran protein filter cake and rice bran-fiber filter cake, they use respectively again washs with described filter cake weighing scale 2 times of water;
E, drying
The rice bran protein filter cake that step D obtains and rice bran-fiber filter cake, the flash drying equipment sold by Jiangsu Xianfeng Drying Engineering Co., Ltd. is adopted to carry out drying respectively under the condition of EAT 160 DEG C and leaving air temp 60 DEG C, until its water content is by weight less than 4%, so obtain described rice bran protein and rice bran-fiber.
Dry product carries out packaging seal as early as possible under the condition controlling packaging humidity.
The rice bran protein that the analytical method adopting this description to describe is prepared the present embodiment and rice bran-fiber have carried out analyzing measuring.The present embodiment prepares 351.3kg rice bran protein (protein content 78.1%, water content 3.7% by 2000kg defatted rice bran raw material (protein content 18.1%, ash content 13.9%, fat 0.95%, total reducing sugar 30.6%, crude fibre 25.7%), namely dry protein content is 81.1%) and 619.7kg rice bran-fiber (fiber content 71.0%, water content 3.3%, namely protein content is 17.0%, ash content 3.5%, fat 0.4%, total reducing sugar 8.1%).
According to the formula that this description describes, can calculate rice bran protein recovery rate by defatted rice bran material quantity and obtained rice bran protein amount is 75.8%.
Embodiment 2: the preparation of high-purity rice bran protein and rice bran-fiber
The implementation step of this embodiment is as follows:
A, pretreatment of raw material
Adopt method for sieving, the tangible foreign matter removal existed in the defatted rice bran raw material (with defatted rice bran raw material total weight, protein content 19.4%, ash content 12.0%, fat 0.9%, total reducing sugar 32.1%, crude fibre 27.8%) that 2000kg is sold by Jiangxi Province and conjunction limited industrial company; Then, the defatted rice bran raw material of screening uses the disintegrating apparatus sold by Wei Dayaohua Machinery Manufacturing Co., Ltd. of Jiangyin City, Hai Ke powder equipment Manufacturing Co., Ltd of Jiangyin City, Longchang Mechanical Mfg. Co., Ltd., Jiangyin to be crushed to grain fineness 150 order, obtains defatted rice bran powder;
B, first time size mixing and enzyme reaction
Add water in the defatted rice bran powder that steps A obtains and size mixing, obtain the feed liquid that defatted rice bran powder concentration is by weight 28%, re-use the 8%NaOH aqueous solution by weight and the pH value of described feed liquid is adjusted to 6.4, then in described feed liquid, add the mesophilicα-diastase sold with Jie Neng section (China) the bioengineering Co., Ltd of defatted rice bran grain weight gauge 0.9%, the re-using the plate type heat exchanger sold by Tianjin General Heat Exchanging Equipment Factory be heated to 70 DEG C containing enzyme feed liquid of mixing, and carry out enzyme digestion reaction 1.0h at this temperature, after enzyme digestion reaction terminates, use above-mentioned plate type heat exchanger that the temperature of its enzyme digestion reaction liquid is reduced to 50 DEG C, obtain first time enzymolysis slurries,
C, second time are sized mixing and enzyme reaction
Use the 8mol/LHCl aqueous solution to be obtained by step B first time enzymolysis slurries pH value be adjusted to 5.2, then in described enzymolysis slurries, add the cellulase sold by Jie Neng section (China) bioengineering Co., Ltd with defatted rice bran grain weight gauge 0.8%, at temperature 52 DEG C, carry out enzyme digestion reaction 4.0h after mixing, obtain second time enzymolysis slurries;
D, separating, washing
The second time enzymolysis slurries obtained by step C are washed by the cyclone sold by Yuanan, Tianzhang City Machinery Co., Ltd., Jiangsu Bang Li biochemical equipment Co., Ltd, Weihai Haiwang Hydrocyclone Co., Ltd., Beijing Huayu Engineering Co., Ltd. of Zh under the following conditions: feed pressure 0.8MPa, underflow pressure 0.4MPa, feed rate 6.0t/h, leaching requirement 6.0t/h; Then obtained eddy flow gently filters respectively through the flame filter press sold by Hangzhou Kun Yuan filtration machinery Co., Ltd with heavy phase, obtain rice bran protein filter cake and rice bran-fiber filter cake, they use respectively again washs with described filter cake weighing scale 4 times of water;
E, drying
The rice bran protein filter cake that step D obtains and rice bran-fiber filter cake, the flash drying equipment sold by Changzhou Lemar Drying Engineering Co., Ltd. is adopted to carry out drying respectively under the condition of EAT 165 DEG C and leaving air temp 65 DEG C, until its water content is by weight less than 4%, so obtain described rice bran protein and rice bran-fiber.
Dry product carries out packaging seal as early as possible under the condition controlling packaging humidity.
The rice bran protein that the analytical method adopting this description to describe is prepared the present embodiment and rice bran-fiber have carried out analyzing measuring.The present embodiment prepares 377.6kg rice bran protein (protein content 78.1%, water content 3.1%, namely dry protein content is 80.6%) and 646.7kg rice bran-fiber (fiber content 72.8%, protein content 13.6%, water content 3.2%, ash content 3.7%, fat 0.8%, total reducing sugar 9.6%) by 2000kg defatted rice bran raw material (protein content 19.4%, ash content 12.0%, fat 0.9%, total reducing sugar 32.1%, crude fibre 27.8%).
According to the formula that this description describes, can calculate rice bran protein recovery rate by defatted rice bran material quantity and obtained rice bran protein amount is 76.0%.
Embodiment 3: the preparation of high-purity rice bran protein and rice bran-fiber
The implementation step of this embodiment is as follows:
A, pretreatment of raw material
Adopt method for sieving, the tangible foreign matter existed in the defatted rice bran raw material (with defatted rice bran raw material total weight, protein content 16.1%, ash content 11.2%, fat 0.75%, total reducing sugar 37.2%, crude fibre 24.3) Baofeng, 2000kg Yutai County grease Co., Ltd sold is removed; Then, the defatted rice bran raw material of screening uses the disintegrating apparatus sold by Wei Dayaohua Machinery Manufacturing Co., Ltd. of Jiangyin City, Hai Ke powder equipment Manufacturing Co., Ltd of Jiangyin City, Longchang Mechanical Mfg. Co., Ltd., Jiangyin to be crushed to grain fineness 120 order, obtains defatted rice bran powder;
B, first time size mixing and enzyme reaction
Add water in the defatted rice bran powder that steps A obtains and size mixing, obtain the feed liquid that defatted rice bran powder concentration is by weight 23%, re-use the 12%NaOH aqueous solution by weight and the pH value of described feed liquid is adjusted to 6.0, then in described feed liquid, add the mesophilicα-diastase sold by the grand mcroorganism Engineering Co., Ltd in Shandong with defatted rice bran grain weight gauge 0.68%, re-using containing enzyme feed liquid of mixing is heated to 66 DEG C by the plate type heat exchanger of Changzhou Sheng Ge heat exchanger Co., Ltd sale, and carry out enzyme digestion reaction 0.6h at this temperature, after enzyme digestion reaction terminates, use above-mentioned plate type heat exchanger that the temperature of its enzyme digestion reaction liquid is reduced to 46 DEG C, obtain first time enzymolysis slurries,
C, second time are sized mixing and enzyme reaction
Use the 12mol/LHCl aqueous solution to be obtained by step B first time enzymolysis slurries pH value be adjusted to 4.9, then in described enzymolysis slurries, add the cellulase sold by the grand mcroorganism Engineering Co., Ltd in Shandong with defatted rice bran grain weight gauge 0.6%, at temperature 49 DEG C, carry out enzyme digestion reaction 3.4h after mixing, obtain second time enzymolysis slurries;
D, separating, washing
The second time enzymolysis slurries obtained by step C are washed by the cyclone sold by Weihai Haiwang Hydrocyclone Co., Ltd., Beijing Huayu Engineering Co., Ltd. of Zh under the following conditions: feed pressure 0.74MPa, underflow pressure 0.34MPa, feed rate 5.6t/h, leaching requirement 5.6t/h; Then obtained eddy flow gently filters respectively through the flame filter press sold by Hangzhou Xingyuan Filter Technology Co., Ltd. with heavy phase, obtain rice bran protein filter cake and rice bran-fiber filter cake, they use respectively again washs with described filter cake weighing scale 3 times of water;
E, drying
The rice bran protein filter cake that step D obtains and rice bran-fiber filter cake, the flash drying equipment sold by Co., Ltd of Changzhou City No.2 Drying Equipment Factory is adopted to carry out drying respectively under the condition of EAT 162 DEG C and leaving air temp 62 DEG C, until its water content is by weight less than 4%, so obtain described rice bran protein and rice bran-fiber.
Dry product carries out packaging seal as early as possible under the condition controlling packaging humidity.
The rice bran protein that the analytical method adopting this description to describe is prepared the present embodiment and rice bran-fiber have carried out analyzing measuring.The present embodiment prepares 302.3kg rice bran protein (protein content 80.1%, water content 3.2%, namely dry protein content is 82.7%) and 587.6kg rice bran-fiber (fiber content 70.3%, water content 3.3%, protein content be 12.6%, ash content 2.9%, fatty 0.4%, total reducing sugar 10.8%) by 2000kg defatted rice bran raw material (protein content 16.1%, ash content 11.2%, fat 0.75%, total reducing sugar 37.2%, crude fibre 24.3).
According to the formula that this description describes, can calculate rice bran protein recovery rate by defatted rice bran material quantity and obtained rice bran protein amount is 75.2%.
Embodiment 4: the preparation of high-purity rice bran protein and rice bran-fiber
The implementation step of this embodiment is as follows:
A, pretreatment of raw material
Adopt method for sieving, the tangible foreign matter existed in the defatted rice bran raw material (with defatted rice bran raw material total weight, protein content 16.1%, ash content 11.2%, fat 0.75%, total reducing sugar 37.2%, crude fibre 24.3) Baofeng, 2000kg Yutai County grease Co., Ltd sold is removed; Then, the defatted rice bran raw material of screening uses the disintegrating apparatus sold by Wei Dayaohua Machinery Manufacturing Co., Ltd. of Jiangyin City, Hai Ke powder equipment Manufacturing Co., Ltd of Jiangyin City, Longchang Mechanical Mfg. Co., Ltd., Jiangyin to be crushed to grain fineness 140 order, obtains defatted rice bran powder;
B, first time size mixing and enzyme reaction
Add water in the defatted rice bran powder that steps A obtains and size mixing, obtain the feed liquid that defatted rice bran powder concentration is by weight 25%, re-use the 10%NaOH aqueous solution by weight and the pH value of described feed liquid is adjusted to 6.5, then in described feed liquid, add the mesophilicα-diastase being believed the sale of (China) Bioisystech Co., Ltd by Novi with defatted rice bran grain weight gauge 0.82%, the re-using the plate type heat exchanger sold by Shanghai Nanhua Transducer Manufacture Co., Ltd. be heated to 68 DEG C containing enzyme feed liquid of mixing, and carry out enzyme digestion reaction 0.8h at this temperature, after enzyme digestion reaction terminates, use above-mentioned plate type heat exchanger that the temperature of its enzyme digestion reaction liquid is reduced to 48 DEG C, obtain first time enzymolysis slurries,
C, second time are sized mixing and enzyme reaction
Use the 10mol/LHCl aqueous solution to be obtained by step B first time enzymolysis slurries pH value be adjusted to 5.0, then add in described enzymolysis slurries and believe by Novi the cellulase that (China) Bioisystech Co., Ltd sells with defatted rice bran grain weight gauge 0.7%, under temperature 50 C, carry out enzyme digestion reaction 3.6h after mixing, obtain second time enzymolysis slurries;
D, separating, washing
The second time enzymolysis slurries obtained by step C are washed by the cyclone sold by Yuanan, Tianzhang City Machinery Co., Ltd. under the following conditions: feed pressure 0.76MPa, underflow pressure 0.36MPa, feed rate 5.8t/h, leaching requirement 5.8t/h; Then obtained eddy flow gently filters respectively through the flame filter press sold by Hangzhou Guo Rui filter press Co., Ltd with heavy phase, obtain rice bran protein filter cake and rice bran-fiber filter cake, they use respectively again washs with described filter cake weighing scale 4 times of water;
E, drying
The rice bran protein filter cake that step D obtains and rice bran-fiber filter cake, the flash drying equipment sold by Jiangsu Xianfeng Drying Engineering Co., Ltd. is adopted to carry out drying respectively under the condition of EAT 164 DEG C and leaving air temp 64 DEG C, until its water content is by weight less than 4%, so obtain described rice bran protein and rice bran-fiber.
Dry product carries out packaging seal as early as possible under the condition controlling packaging humidity.
The rice bran protein that the analytical method adopting this description to describe is prepared the present embodiment and rice bran-fiber have carried out analyzing measuring.The present embodiment prepares 309.7kg rice bran protein (protein content 78.5%, water content 3.5% by 2000kg defatted rice bran raw material (protein content 16.1%, ash content 11.2%, fat 0.75%, total reducing sugar 37.2%, crude fibre 24.3%), namely dry protein content is 81.3%) and 555.6kg rice bran-fiber (fiber content 74.4%, water content 3.4%, protein content be 11.7%, ash content 3.4%, fat 0.6%, total reducing sugar 10.5%).
According to the formula that this description describes, can calculate rice bran protein recovery rate by defatted rice bran material quantity and obtained rice bran protein amount is 75.5%.
Embodiment 5: the preparation of high-purity rice bran protein and rice bran-fiber
The implementation step of this embodiment is as follows:
A, pretreatment of raw material
Adopt method for sieving, the tangible foreign matter existed in the defatted rice bran raw material (with defatted rice bran raw material total weight, protein content 18.1%, ash content 13.9%, fat 0.95%, total reducing sugar 30.6%, crude fibre 25.7%) 2000kg Jiangxi peasants who dig gold meter Ye Co., Ltd sold is removed; Then, the defatted rice bran raw material of screening uses the disintegrating apparatus sold by Wei Dayaohua Machinery Manufacturing Co., Ltd. of Jiangyin City, Hai Ke powder equipment Manufacturing Co., Ltd of Jiangyin City, Longchang Mechanical Mfg. Co., Ltd., Jiangyin to be crushed to grain fineness 140 order, obtains defatted rice bran powder;
B, first time size mixing and enzyme reaction
Add water in the defatted rice bran powder that steps A obtains and size mixing, obtain the feed liquid that defatted rice bran powder concentration is by weight 23.5%, re-use the 8%NaOH aqueous solution by weight and the pH value of described feed liquid is adjusted to 6.2, then in described feed liquid, add the mesophilicα-diastase being believed the sale of (China) Bioisystech Co., Ltd by Novi with defatted rice bran grain weight gauge 0.72%, the re-using the plate type heat exchanger sold by Shanghai Nanhua Transducer Manufacture Co., Ltd. be heated to 67 DEG C containing enzyme feed liquid of mixing, and carry out enzyme digestion reaction 0.7h at this temperature, after enzyme digestion reaction terminates, use above-mentioned plate type heat exchanger that the temperature of its enzyme digestion reaction liquid is reduced to 47 DEG C, obtain first time enzymolysis slurries,
C, second time are sized mixing and enzyme reaction
Use the 8mol/LHCl aqueous solution to be obtained by step B first time enzymolysis slurries pH value be adjusted to 4.8, then add in described enzymolysis slurries and believe by Novi the cellulase that (China) Bioisystech Co., Ltd sells with defatted rice bran grain weight gauge 0.62%, at temperature 48 DEG C, carry out enzyme digestion reaction 3.2h after mixing, obtain second time enzymolysis slurries;
D, separating, washing
The second time enzymolysis slurries obtained by step C are washed by the cyclone sold by Yuanan, Tianzhang City Machinery Co., Ltd. under the following conditions: feed pressure 0.72MPa, underflow pressure 0.32MPa, feed rate 5.7t/h, leaching requirement 5.7t/h; Then obtained eddy flow gently filters respectively through the flame filter press sold by Hangzhou Guo Rui filter press Co., Ltd with heavy phase, obtains rice bran protein filter cake and rice bran-fiber filter cake, and they use respectively again washs with described filter cake weighing scale 3 times of water;
E, drying
The rice bran protein filter cake that step D obtains and rice bran-fiber filter cake, the flash drying equipment sold by Jiangsu Xianfeng Drying Engineering Co., Ltd. is adopted to carry out drying respectively under the condition of EAT 160 DEG C and leaving air temp 60 DEG C, until its water content is by weight less than 4%, so obtain described rice bran protein and rice bran-fiber.
Dry product carries out packaging seal as early as possible under the condition controlling packaging humidity.
The rice bran protein that the analytical method adopting this description to describe is prepared the present embodiment and rice bran-fiber have carried out analyzing measuring.The present embodiment prepares 340.1kg rice bran protein (protein content 77.9%%, water content 3.7% by 2000kg defatted rice bran raw material (protein content 18.1%, ash content 13.9%, fat 0.95%, total reducing sugar 30.6%, crude fibre 25.7%), namely dry protein content is 80.9%) and 565.0kg rice bran-fiber (fiber content 72.0%, water content 3.6%, namely protein content is 12.9%, ash content 3.2%, fat 0.7%, total reducing sugar 9.9%).
According to the formula that this description describes, can calculate rice bran protein recovery rate by defatted rice bran material quantity and obtained rice bran protein amount is 76.0%.
Embodiment 6: the preparation of high-purity rice bran protein and rice bran-fiber
The implementation step of this embodiment is as follows:
A, pretreatment of raw material
Adopt method for sieving, the tangible foreign matter removal existed in the defatted rice bran raw material (with defatted rice bran raw material total weight, protein content 19.4%, ash content 12.0%, fat 0.9%, total reducing sugar 32.1%, crude fibre 27.8%) that 2000kg Jiangxi Province and conjunction limited industrial company are sold; Then, the defatted rice bran raw material of screening uses the disintegrating apparatus sold by Wei Dayaohua Machinery Manufacturing Co., Ltd. of Jiangyin City, Hai Ke powder equipment Manufacturing Co., Ltd of Jiangyin City, Longchang Mechanical Mfg. Co., Ltd., Jiangyin to be crushed to grain fineness 120 order, obtains defatted rice bran powder;
B, first time size mixing and enzyme reaction
Add water in the defatted rice bran powder that steps A obtains and size mixing, obtain the feed liquid that defatted rice bran powder concentration is by weight 24.5%, re-use the 12%NaOH aqueous solution by weight and the pH value of described feed liquid is adjusted to 6.4, then in described feed liquid, add the mesophilicα-diastase being believed the sale of (China) Bioisystech Co., Ltd by Novi with defatted rice bran grain weight gauge 0.78%, the re-using the plate type heat exchanger sold by Shanghai Nanhua Transducer Manufacture Co., Ltd. be heated to 68 DEG C containing enzyme feed liquid of mixing, and carry out enzyme digestion reaction 0.8h at this temperature, after enzyme digestion reaction terminates, use above-mentioned plate type heat exchanger that the temperature of its enzyme digestion reaction liquid is reduced to 48 DEG C, obtain first time enzymolysis slurries,
C, second time are sized mixing and enzyme reaction
Use the 12mol/LHCl aqueous solution to be obtained by step B first time enzymolysis slurries pH value be adjusted to 5.2, then add in described enzymolysis slurries and believe by Novi the cellulase that (China) Bioisystech Co., Ltd sells with defatted rice bran grain weight gauge 0.65%, at temperature 52 DEG C, carry out enzyme digestion reaction 3.8h after mixing, obtain second time enzymolysis slurries;
D, separating, washing
The second time enzymolysis slurries obtained by step C are washed by the cyclone sold by Yuanan, Tianzhang City Machinery Co., Ltd. under the following conditions: feed pressure 0.78MPa, underflow pressure 0.38MPa, feed rate 5.9t/h, leaching requirement 5.9t/h; Then obtained eddy flow gently filters respectively through the flame filter press sold by Hangzhou Guo Rui filter press Co., Ltd with heavy phase, obtains rice bran protein filter cake and rice bran-fiber filter cake, and they use respectively again washs with described filter cake weighing scale 2 times of water;
E, drying
The rice bran protein filter cake that step D obtains and rice bran-fiber filter cake, the flash drying equipment sold by Jiangsu Xianfeng Drying Engineering Co., Ltd. is adopted to carry out drying respectively under the condition of EAT 165 DEG C and leaving air temp 65 DEG C, until its water content is by weight less than 4%, so obtain described rice bran protein and rice bran-fiber.
Dry product carries out packaging seal as early as possible under the condition controlling packaging humidity.
The rice bran protein that the analytical method adopting this description to describe is prepared the present embodiment and rice bran-fiber have carried out analyzing measuring.The present embodiment by 2000kg defatted rice bran raw material (with defatted rice bran raw material total weight, protein content 19.4%, ash content 12.0%, fat 0.9%, total reducing sugar 32.1%, crude fibre 27.8%) prepare 357.7kg rice bran protein (protein content 78.8%, water content 3.9%, namely dry protein content is 82.0%) and 541.7kg rice bran-fiber (fiber content 73.5%, water content 3.8%, namely protein content is 11.5%, ash content 3.0%, fat 0.4%, total reducing sugar 9.0%).
According to the formula that this description describes, can calculate rice bran protein recovery rate by defatted rice bran material quantity and obtained rice bran protein amount is 75.6%.
Comparative example 1: prepare high-purity rice bran protein and rice bran-fiber according to prior art
With reference to the preparation method that CN102178151A describes, prepare rice bran dietary fiber and rice bran protein by defatted rice bran coproduction.
The implementation step of this comparative example is as follows:
A, pulverizing
Defatted rice bran is pulverized, is filtered by 80 eye mesh screens;
B, immersion
Be the NaOH solution immersion 60min of 0.15mol/L by the defatted rice bran concentration of pulverizing, obtain soak;
C, centrifugal: use soak and carry out centrifuge process, centrifuge speed is 4500r/min, centrifugation time is 10min, obtains the first filter residue, the first protein suspension;
D, complex enzyme hydrolysis:
A, the end liquid that substrate and buffer solution are mixed, substrate is defatted rice bran, buffer solution is mass percent concentration is the sodium dihydrogen phosphate of 0.1mol/L and the phosphate buffer of sodium hydrogen phosphate, the percentage by weight of end liquid is 21%, then by the first filter residue, complex enzyme adds in end liquid, first time enzymolysis is carried out after mixing, temperature during first time enzymolysis is 45 DEG C, time is 2h, pH is 5.8, obtain first time enzymolysis product, the complex enzyme of described every 100 weight portions comprises the cellulase of 62 weight portions, the compound protease of 26 weight portions and the phytase of 12 weight portions, and cellulose enzyme activity is 50,000 U/g, the work of compound protease enzyme is 50,000 U/g, phytase activity is 5000U/g, in the end liquid of every 100 weight portions, first filter residue, the addition of complex enzyme is respectively 22 weight portions, 4.3 weight portion,
B, the high-temperatureα-amylase being 20,000 U/g by enzyme work add in first time enzymolysis product, carry out second time enzymolysis, temperature during second time enzymolysis is 90 DEG C, time is 120min, pH is 5.7, obtain second time enzymolysis product, relative to the substrate in the step a of every 100 weight portions, high-temperatureα-amylase addition is 3.1 weight portions;
C, by second time enzymolysis product 100 DEG C, after the enzyme that goes out under the condition of 20min, carrying out centrifugal treating, obtain the second filter residue, the second protein filtrate, the rotating speed of the centrifuge that centrifugal treating uses is 4500r/min, centrifugation time is 10min;
E, produce rice bran dietary fiber: after being rinsed by the second filter residue water, drying process both rice bran dietary fiber, dry treatment temperature is 120 DEG C;
F, produce rice bran protein; By the first protein filtrate and the mixing of the second protein filtrate, in the ratio of 3g/100mL, active carbon is added in the mixed liquor of the first protein filtrate and the second protein filtrate, after described mixed liquor adsorption bleaching 45min, carbon-point formula pressure filter is adopted to filter to remove active carbon, with HCl, the pH of described mixed liquor is adjusted to 4.6 again, carry out isoelectric precipitation 45min, then adopt centrifuge to carry out centrifugal treating to it, the rotating speed of centrifuge is 4500r/min, centrifugation time is 10min; Taking precipitate vacuum, be less than 50 DEG C of conditions under carry out drying process, obtain rice bran protein.
The results are shown in Table 1 for embodiment 1-6 and comparative example 1.
The result of implementation of table 1: embodiment 1-4 and comparative example 1
(1) rice bran protein
(2) rice bran-fiber
Can be known by the analysis result of table 1 and find out, compared with comparative example 1, the dry protein content of embodiment 1-6 gained rice bran protein all reaches more than 80%, protein extracting ratio is greater than 75%, enzymolysis is not had to destroy to albumen, and non-protein impurity content is lower in product, synchronously can obtain the rice bran-fiber product of the content with lower ash content and fat, the molten acid of alkali is adopted to sink in comparative example 1 and the method for enzymolysis protein, the auxiliary material such as soda acid and enzyme preparation materials are larger, cost is higher, albumen has certain bad flavor through enzymolysis and produces, and protein extracting ratio declines, the content of the composition in the substep rice bran protein of gained and rice bran-fiber beyond albumen and fiber is not addressed.
Claims (9)
1. a preparation method for high-purity rice bran protein and rice bran-fiber, is characterized in that the step of described method is as follows:
A, pretreatment of raw material
Adopt method for sieving, by protein content by weight 16% ~ 20% defatted rice bran raw material in the tangible foreign matter that exists remove; Then, the defatted rice bran raw material of screening uses disintegrating apparatus to pulverize, and obtains defatted rice bran powder; Wherein, use micronizer that defatted rice bran raw meal is broken to grain fineness 120 ~ 150 order;
B, first time size mixing and enzyme reaction
Add water in the defatted rice bran powder that steps A obtains and size mixing, obtain the feed liquid that defatted rice bran powder concentration is by weight 20% ~ 28%, re-use 8 ~ 12%NaOH aqueous solution by weight and the pH value of described feed liquid is adjusted to 6.0 ~ 6.5, then add in described feed liquid with the mesophilicα-diastase of defatted rice bran grain weight gauge 0.6 ~ 0.9%, the enzyme feed liquid that contains of mixing is heated to 65 ~ 70 DEG C again, and carry out enzyme digestion reaction 0.5 ~ 1.0h at this temperature, terminate the temperature of its enzyme digestion reaction liquid to be reduced to 45 ~ 50 DEG C at enzyme digestion reaction, obtain first time enzymolysis slurries;
C, second time are sized mixing and enzyme reaction
Use 0.8 ~ 1.2mol/LHCl aqueous solution to be obtained by step B first time enzymolysis slurries pH value be adjusted to 4.8 ~ 5.2, then add in described enzymolysis slurries with defatted rice bran grain weight gauge 0.5% ~ 0.8% cellulase, at temperature 48 ~ 52 DEG C, carry out enzyme digestion reaction 3.0 ~ 4.0h after mixing, obtain second time enzymolysis slurries;
D, separating, washing
The second time enzymolysis slurries obtained by step C are washed by cyclone under the following conditions: feed pressure 0.7 ~ 0.8MPa, underflow pressure 0.3 ~ 0.4MPa, feed rate 5.5 ~ 6.0t/h, leaching requirement 5.5 ~ 6.0t/h; Then obtained eddy flow gently filters respectively through flame filter press with heavy phase, obtains rice bran protein filter cake and rice bran-fiber filter cake, and they use respectively again washs with described filter cake weighing scale 2 ~ 4 times of water;
E, drying
The rice bran protein filter cake that step D obtains and rice bran-fiber filter cake, flash drying equipment is adopted to carry out drying under the condition of EAT 160 ~ 165 DEG C and leaving air temp 60 ~ 65 DEG C, until its water content is with dry weight basis less than 4%, so obtain described rice bran protein and rice bran-fiber.
2. preparation method according to claim 1, is characterized in that in stepb, and the concentration of defatted rice bran powder liquid is by weight 23% ~ 25%.
3. preparation method according to claim 1, is characterized in that in stepb, and the addition of described mesophilicα-diastase is with defatted rice bran grain weight gauge 0.68 ~ 0.82%.
4. preparation method according to claim 1, is characterized in that in stepb, and defatted rice bran powder liquid and mesophilicα-diastase carry out enzyme digestion reaction 0.6 ~ 0.8h at temperature 66 ~ 68 DEG C.
5. preparation method according to claim 1, it is characterized in that in stepb, use plate type heat exchanger that defatted rice bran powder liquid and mesophilicα-diastase mixture are heated to 66 ~ 68 DEG C, after enzyme digestion reaction terminates, the temperature of its enzyme digestion reaction liquid is reduced to 46 ~ 48 DEG C.
6. preparation method according to claim 1, is characterized in that in step C, and second time enzyme digestion reaction carries out reaction 3.4 ~ 3.6h at temperature 49 ~ 50 DEG C.
7. preparation method according to claim 1, is characterized in that in step C, and the addition of described cellulase is with defatted rice bran grain weight gauge 0.6% ~ 0.7%.
8. preparation method according to claim 1, it is characterized in that in step D, second time enzymolysis slurries use cyclone to wash under the condition of feed pressure 0.74 ~ 0.76MPa, underflow pressure 0.34 ~ 0.36MPa, feed rate 5.6 ~ 5.8t/h, leaching requirement 5.6 ~ 5.8t/h.
9. the product that described in claim, preparation method obtains any one of claim 1-8, is characterized in that with described rice bran protein total weight, and the protein content of described rice bran protein is more than 80%, content of ashes less than 3% and fat content less than 0.5%; The recovery rate of described rice bran protein is more than 75%; With described rice bran-fiber total weight, the content of ashes of described rice bran-fiber is less than 4%, fat content less than 1%.
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| CN106906267A (en) * | 2017-04-17 | 2017-06-30 | 江南大学 | A kind of method that utilization fermentation method prepares rice bran protein polypeptide from defatted rice bran |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1423532A (en) * | 2000-02-15 | 2003-06-11 | 利波詹尼克斯公司 | Enzymatic processing of rice bran to produce edible products |
| CN1483349A (en) * | 2002-09-22 | 2004-03-24 | 于铁梁 | Method for preparing soluble dietary fibre |
| CN102178151A (en) * | 2011-04-22 | 2011-09-14 | 淮南市楚丰工贸有限公司 | Method for co-producing rice bran dietary fiber and rice bran protein by utilizing defatted rice bran |
| CN102356845A (en) * | 2011-10-29 | 2012-02-22 | 安徽燕之坊食品有限公司 | Preparation method of rice bran nutrient |
| CN102948607A (en) * | 2012-12-13 | 2013-03-06 | 哈尔滨商业大学 | Method for preparing rice bran protein through steam explosion-compound enzyme method |
-
2013
- 2013-09-11 CN CN201310413709.XA patent/CN103478400B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1423532A (en) * | 2000-02-15 | 2003-06-11 | 利波詹尼克斯公司 | Enzymatic processing of rice bran to produce edible products |
| CN1483349A (en) * | 2002-09-22 | 2004-03-24 | 于铁梁 | Method for preparing soluble dietary fibre |
| CN102178151A (en) * | 2011-04-22 | 2011-09-14 | 淮南市楚丰工贸有限公司 | Method for co-producing rice bran dietary fiber and rice bran protein by utilizing defatted rice bran |
| CN102356845A (en) * | 2011-10-29 | 2012-02-22 | 安徽燕之坊食品有限公司 | Preparation method of rice bran nutrient |
| CN102948607A (en) * | 2012-12-13 | 2013-03-06 | 哈尔滨商业大学 | Method for preparing rice bran protein through steam explosion-compound enzyme method |
Non-Patent Citations (3)
| Title |
|---|
| 张敏 等.米糠蛋白和米糠纤维在焙烤食品中的应用.《四川粮油科技》.2003,第38-44页. * |
| 池爱平 等.脱脂米糠酶法水解制备米糠营养素工艺研究.《食品科学》.2006,第27卷(第12期),第339-343页. * |
| 薛勇.水力旋流器.《离心捕集原理与设备》.科学出版社,2011,第185页. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906267A (en) * | 2017-04-17 | 2017-06-30 | 江南大学 | A kind of method that utilization fermentation method prepares rice bran protein polypeptide from defatted rice bran |
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