CN106906264A - A kind of method for preparing bacteria cellulose as carbon source by the use of tea grounds - Google Patents
A kind of method for preparing bacteria cellulose as carbon source by the use of tea grounds Download PDFInfo
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Abstract
The invention discloses a kind of method for preparing bacteria cellulose as carbon source by the use of tea grounds, including：（1）Zymotic fluid is prepared using tea grounds：Tea grounds slurries are prepared, addition inorganic salts, regulation pH prepare fermentation culture, sterilize standby；（2）The preparation of tea grounds saccharified liquid：Saccharification is carried out to tea grounds zymotic fluid using cellulose-degrading bacteria, tea grounds saccharified liquid is obtained；（3）The preparation of fermentation medium：Saccharified liquid adds nitrogen source and phosphorus source is configured to fermentation medium；（4）Access the seed liquor that bacteria cellulose produces bacterial strain, fermented prepared bacteria cellulose.Carbon source of the present invention by the use of a large amount of discarded tea grounds as fermentation medium for bacterial cellulose, can effectively reduce the raw materials for production cost of bacteria cellulose, promote the large-scale production of bacteria cellulose.
Description
Technical field
The invention belongs to bacteria cellulose preparation field, and in particular to a kind of to prepare bacterial fibers as carbon source by the use of tea grounds
The method of element.
Background technology
Bacteria cellulose（Bacterial Cellulose, BC）, also referred to as micro organism cellulose；Chemical formula is
(C6H10O)n, it is a kind of straight-chain polysaccharide biomass formed with β-Isosorbide-5-Nitrae glycosidic bond connection by β-D-Glucose monomer.Due to gathering around
There are ultra-fine three-dimensional net structure, substantial amounts of nanoscale pore-size distribution, very big specific surface area and abundant surface hydroxyl base
Group, therefore it is widely used in food, industry, papermaking and the field such as medical.
Compared with plant cellulose, adjacent 6 carbon atoms of glucopyranose are in stable chair structure in BC, and plant
Fibres element molecule has helical structure.In its three-dimensional structure, between same straight chain, same plane difference straight chain and up and down difference
There is hydrogen bond to be closely crosslinked between straight chain, fine and close cross-linked structure is formed, although this causes BC with plant in nature or sea
The native cellulose that algae produces has identical molecular structure unit, but with many unique properties：（1）High-purity, with plant
Fibres element is compared, and BC is free of by that can obtain the cellulose that purity is up to more than 99% after the simple process such as separating-purifying
There is the association product such as lignin, hemicellulose and pectin；（2）With high-crystallinity, 95% is reached as high as, and plant cellulose
Crystallinity typically only 65%, and BC also has the degree of polymerization very high；（3）BC has hyperfine network structure, and BC fibers are by diameter
The microfibre of 3-4 nm is acted on by intramolecular and intermolecular oxygen key and winding is combined into the thick fibre bundles of 40-60 nm, and phase
Mutually interweave and form hyperfine three-dimensional space net structure；（5）Very strong moisture holding capacity, the mesh nanometer of densification is possessed due to BC
Structure, specific surface area is big, and abundant hydrophilic radical is contained on surface, and this causes that BC has water retention property higher, undried
BC WRV values up to more than 1000%, the moisture holding capacity after freeze-drying is still above 600%；（6）Biosynthesis has adjustable
Control property, the economic worth of BC is mainly reflected in that can to change its by regulating and controlling the environment of its biosynthesis original structural
Matter, so as to adapt to the demand of various industrial productions and academic research；（7）BC has well adapting to property and bio-compatible higher
Property, and with good biodegradability, can be degraded under corresponding physical and chemical condition, it is environmentally friendly, will not produce
Secondary pollution.
In view of the good characteristic of bacteria cellulose, bacteria cellulose has extensive and special purposes, is led in medical material
Domain, bacteria cellulose can be used for carrier of synthesis of artificial skin, artificial blood vessel, surgical dressing, slow releasing pharmaceutical etc.；In food work
Journey field, bacteria cellulose can serve as a kind of food consumption in itself, such as be commonly called as coconut palm fruit or nata de coco；In addition, BC may be used also
As the thickener in food industry, forming agent, additive etc.；In terms of paper industry, the addition of bacteria cellulose can be with
Paper tensile strength and bursting strength are improved, air permeability is reduced, tearability etc. is improved；Can be used as the super performance of production in field of audio
Acoustical vibration film；In Material Field, BC nanofibers and other macromolecules, organic or inorganic molecule doped and compounded can be obtained
Obtain various new functional composite materials.
The extensive obstacle using bacteria cellulose is mainly it and yields poorly at present, and high cost, price is defeated by general fibre
Element, therefore research focused in the new carbon source of searching, finds cheap suitable, can be reduced production cost and can be improved fibre
Tie up the yield of element.
Tealeaves produces a large amount of tealeaves discarded objects, i.e., so-called tea grounds in production and deep-processing process.The master of China's tea grounds
Originating has two：One is the tea grounds formed after the biochemical compositions such as industrial abstract Tea Polyphenols；Two is shape in tea beverage industry
Into tea grounds.Nutritional ingredient is relatively enriched in the tea grounds formed by tea beverage industry, and tea beverage industry tea is focused on here
The exploitation of slag.Although China's tea beverage industry is started late, growth momentum is very powerful, and tea beverage annual production is doubled and redoubled.
From 1997, tea beverage yield was doubled and redoubled, and one action in 2000 exceedes fruit juice, fruit-flavored beverage and ranks the 3rd of various types of beverages
Position.However, it is only 0.4 L that the interior drinks tea beverage equal year at present, Japan drinks tea beverage year for 20~30 L per capita, also
It is to say, should also there be more than 30 times of growing space in market to tea beverage interiorly.4 production of tea drinks lines that Wahaha is introduced, year
5000 tons of dry tea of digestion, this shows to produce tea grounds more than 4000 tons；So calculate, at present the tea produced by production tea beverage
, more than 40,000 tons, as increased by 30 times, tea grounds will be more than 1,200,000 tons for output in several years in the case where connecing for slag.So many tea grounds mesh
It is preceding to be all taken as industrial waste to abandon substantially, only cause the huge waste of resource by as Term Fertilization to field on a small quantity.Cause
This, tea grounds is turned waste into wealth, and reluctant tea grounds waste material is turned into a kind of high-quality novel fodder, is conducive to the guarantor of ecological environment
Shield；It is also beneficial to reduce entreprise cost, improves the market competitiveness of enterprise.
Tea beverage tea grounds (instant tea grounds) refers to the residue after the tealeaves instant composition of extraction, through analysis shows：Crude fibre is up to
17%~19%, crude protein content is up to 16%~18%, and lysine and methionine content are respectively 1.5%~2% and 0.5~0.7%,
Also containing a certain amount of vitamin, Tea Polyphenols, caffeine and a small amount of Tea Saponin etc., still there is value higher；A large amount of tea
Slag is usually taken as waste material to abandon, and causes the significant wastage and environmental destruction of resource.Therefore can be filled if any a kind of technology
Point these existing waste resources are utilized, be that carbon source prepares bacteria cellulose with it, lift its surcharge, not only bacterial fibers
The production of element provides new way, and can improve the economic use value of tea grounds.
The content of the invention
To solve the problems, such as that above-mentioned bacteria cellulose production cost is high, tea grounds utilization rate is low, the present invention provides a kind of utilization
The method that tea grounds prepares bacteria cellulose as carbon source, the method have raw material sources extensively, process is simple, energy consumption be low, reaction
Rapid the advantages of；And the culture medium carbon source quality of preparation is good, price is low, is suitable for industrial production；In the application of bacteria cellulose
There is good application prospect in field.
To achieve the above object, the present invention is adopted the following technical scheme that：
A kind of method for preparing bacteria cellulose as carbon source by the use of tea grounds, it is comprised the following specific steps that：
（1）Zymotic fluid is prepared using tea grounds：According to solid-to-liquid ratio 1 after tea grounds is cleaned:10 mix with water and squeeze the juice, and tea is smashed to obtain at a high speed
Dreg slurry, adds inorganic salts, and regulation pH is 4.0-6.0, obtains the fermentation medium with tea grounds slurry as principal component, loads triangular flask
In, 121 DEG C sterilizing 20-30min, naturally cool to 20-30 DEG C it is standby；
（2）The preparation of tea grounds saccharified liquid：In step（1）Cellulose-degrading bacteria is inoculated with the tea grounds fermentation medium of preparation, is carried out
Saccharification of cellulose is hydrolyzed, and obtains tea grounds saccharified liquid；Its concrete technology is：The cellulose-degrading bacteria of activated culture is accessed into step
（1）Saccharification is carried out in the triangular flask.
（3）The preparation of fermentation medium：Using above-mentioned tea grounds saccharified liquid as culture medium carbon source, nitrogen source and phosphorus source are added, adjusted
Section pH is 5.0-6.0, is configured to fermentation medium, is placed in triangular flask, is sterilized standby；
（4）Fermentation prepares bacteria cellulose：The seed liquor that bacteria cellulose produces bacterial strain is accessed, by the shaken cultivation of 1-3 days
With 3-20 days quiescent cultures, bacteria cellulose is obtained.
Wherein, the step（1）In tea grounds be the tea grounds discarded in Tea Processing production process.
The step（1）In inorganic salts be KH2PO4、K2HPO4、NaCl、MgSO4、ZnSO4、FeCl3In one kind or several
Kind.
The step（2）In cellulose-degrading bacteria be Cytophage（Cytophaga）, bacillus
（Bacillus）, hot acid Pseudomonas（Acidothermus）, hot rod Pseudomonas（Caldibacillus）, Cellvibrio
（Cellvlbrio）, pseudomonas（Pseudomonas）And Erwinia（Erwinia）In one or more.
The step（2）In saccharification condition be 30-45 DEG C, be saccharified water under conditions of 120 ~ 180rpm of shaking speed
Solution 36-120 h.
The step（3）In tea grounds saccharified liquid add appropriate nitrogen source and phosphorus source first, adjust pH, be placed in triangular flask,
121 DEG C of sterilizing 20-30 min, after being cooled to room temperature, access the seed liquor that bacteria cellulose produces bacterial strain, using elder generation in 120-
Dynamic oscillation culture 1 ~ 3 day under 240 rpm rotating speeds, then the quiescent culture fermentation form of being association of activity and inertia of 3 ~ 20 days is obtained bacterial fibers
Element.
The step（3）In nitrogen source for 0.1-1.0 wt% yeast extract and 0.1-0.5 wt% tryptone；Or
Person is the ammonium sulfate or corn pulp or brewer's wort of 0.1-2.0 wt%.
The step（3）In phosphorus source for 0.1-1.0 wt% 0.1-1.0 wt% KH2PO4、K2HPO4Or NaH2PO4Or
Na2HPO4。
The step（4）Middle condition of culture is 20-30 DEG C, shaken cultivation 1-3 days under 120-240rpm rotating speeds, then quiet
Put culture 3-20 days.
The step（4）In bacteria cellulose production bacterial strain be acetic acid Pseudomonas, gluconobacter suboxydans category, glucose vinegar bar
Pseudomonas, glucose oxidation and bacillus, rhizobium, Sarcina, pseudomonas, achromobacter, Alcaligenes, gas bacillus
One kind or several in category, azotobacter, Agrobacterium, onion pseudomonas sp, campylobacter jejuni, gluconate pyracetobacillus
Kind.
The step（4）The inoculum concentration of middle bacteria cellulose production bacterial strain is 3-15%vol.
Remarkable advantage of the invention is：
（1）With tea grounds as raw material, with cheap, wide material sources, pretreatment hydrolysis process has simple, energy consumption to the present invention
It is low, hydrolyzate sugared content is high, the advantages of be swift in response.Also contain the units such as a small amount of proteins,vitamins,minerals in tea grounds
Element, can promote the synthesis of BC.
（2）Tea grounds is a kind of regenerative resource, and it is not only that its recycling is provided to prepare bacteria cellulose for fermentation
One effective path, and for the industrialization of bacteria cellulose provides cheap new raw material.
（3）The cost of material of the bacteria cellulose that tea grounds hydrolyzate of the present invention through processing is produced is greatly lowered, thin
The production field of fungin has a good application prospect.
Brief description of the drawings
The SEM scanning patterns of the corresponding bacteria cellulose of Fig. 1 embodiment of the present invention 1；
The XRD spectrum of the corresponding bacteria cellulose of Fig. 2 embodiment of the present invention 1；
The FTIR collection of illustrative plates of the corresponding bacteria cellulose of Fig. 3 embodiment of the present invention 1.
Specific embodiment
The present invention provides a kind of method for preparing bacteria cellulose as carbon source by the use of tea grounds, for make the purpose of the present invention,
Technical scheme and effect are clearer, clear and definite, with reference to specific embodiment, the present invention are expanded on further.It should be understood that these realities
Example is applied to be only illustrative of the invention and is not intended to limit the scope of the invention.In addition, it is to be understood that reading instruction of the present invention
After content, those skilled in the art can make various changes or modifications to the present invention, and these equivalent form of values equally fall within this Shen
Please appended claims limited range.
Embodiment 1
1. zymotic fluid is prepared using tea grounds：
Tea grounds is chosen first and water presses solid-liquid mass ratio 1:10 are placed in juice extractor, it is smashed at a high speed and is stirred, and obtain
Tea grounds slurries, addition trace element, make its ultimate density be： KH2PO4 0.6 g/L、NaCl 1 g/L、MgSO4 0.1 g/L、
ZnSO4 0.25 mg/L、FeCl30.01 g/L, adjusts pH to 4.0, obtains the fermentation medium with tea grounds juice as principal component, dress
Enter in triangular flask, sterilize 20 min at 121 DEG C, is cooled to room temperature standby.
2. the preparation of tea grounds saccharified liquid：
By Cytophage（Cytophaga）, bacillus（Bacillus）, Cellvibrio（Cellvlbrio）As fibre
The plain degradation bacteria of dimension, in the triangular flask that the cellulose-degrading bacteria access that will amplify culture through two-stage is matched somebody with somebody honest material and sterilized and cooled down,
In 35 DEG C of temperature, the h of saccharification 96 under the fermentation condition of the rpm of shaking speed 120 is obtained tea grounds saccharified liquid.
3. the preparation of fermentation medium：
Using above-mentioned tea grounds saccharified liquid as culture medium carbon source, the tryptone of the yeast extract and 0.3 wt% of adding 0.5 wt% is made
It is nitrogen source and the Na of 0.6 wt%2HPO4As phosphorus source；PH is to 5.0 for regulation, is configured to fermentation medium, is placed in triangular flask,
121 DEG C of 20 min of sterilizing, are cooled to standby after room temperature.
4. fermentation prepares bacteria cellulose：
Gluconate pyracetobacillus, Agrobacterium, acetic acid Pseudomonas are accessed into liquid seed culture medium and prepares bacteria cellulose production
The seed liquor of bacterial strain, is then transferred to fermentation medium by the inoculum concentration of percent by volume 3%, in 20 DEG C with 180 rpm rotating speeds
Lower dynamic oscillation culture 3 days, then quiescent culture fermentation in 3 days is obtained bacteria cellulose.
Embodiment 2
1. zymotic fluid is prepared using tea grounds
Tea grounds is chosen first and water presses solid-liquid mass ratio 1：10 are placed in juice extractor, it is smashed at a high speed and is stirred, and obtain
Tea grounds slurries, addition trace element, make its ultimate density be：0.4 g/L KH2PO4、0.8 g/L NaCl、0.2 g/L
MgSO4, pH to 4.5 is adjusted, the fermentation medium with tea grounds juice as principal component is obtained, it is fitted into triangular flask, in 121 DEG C of sterilizings
25 min, are cooled to room temperature standby.
2. the preparation of tea grounds saccharified liquid：
By hot acid Pseudomonas（Acidothermus）, pseudomonas（Pseudomonas）And Erwinia（Erwinia）As fibre
The plain degradation bacteria of dimension, in the triangular flask that the cellulose-degrading bacteria access that will amplify culture through two-stage is matched somebody with somebody honest material and sterilized and cooled down,
In 35 DEG C of temperature, saccharification 72h under the fermentation condition of the rpm of shaking speed 180 is obtained tea grounds saccharified liquid.
3. the preparation of fermentation medium：
Using above-mentioned tea grounds saccharified liquid as culture medium carbon source, the ammonium sulfate of 1.0 wt% is added as nitrogen source and 0.5 wt%
K2HPO4As phosphorus source；PH is to 5.5 for regulation, is configured to fermentation medium, is placed in triangular flask, 121 DEG C of 20 min of sterilizing, cooling
It is standby after to room temperature.
4. fermentation prepares bacteria cellulose：
Acetic acid Pseudomonas, gluconobacter suboxydans category, gluconic acid Acetobacter are accessed liquid seed culture medium and prepare bacteria cellulose
The seed liquor of bacterial strain is produced, then fermentation medium is transferred to by the inoculum concentration of percent by volume 6%, in 30 DEG C, 150 rpm shake
After swinging culture 2 days, then quiescent culture fermentation in 20 days is obtained bacteria cellulose.
Embodiment 3
1. zymotic fluid is prepared using tea grounds
Tea grounds is chosen first and water presses solid-liquid mass ratio 1：10 are placed in juice extractor, it is smashed at a high speed and is stirred, and obtain
Tea grounds slurries, addition trace element, make its ultimate density be：0.9 g/L KH2PO4、1.2 g/L NaCl、0.02g/L FeCl3
Regulation pH to 5.0, obtains the fermentation medium with tea grounds juice as principal component, is fitted into triangular flask, and sterilize 20 min at 121 DEG C,
It is cooled to room temperature standby.
2. the preparation of tea grounds saccharified liquid：
By Cellvibrio（Cellvlbrio）, pseudomonas（Pseudomonas）And Erwinia（Erwinia）As fibre
The plain degradation bacteria of dimension, in the triangular flask that the cellulose-degrading bacteria access that will amplify culture through two-stage is matched somebody with somebody honest material and sterilized and cooled down,
In 30 DEG C of temperature, the h of saccharification 72 under the fermentation condition of the rpm of shaking speed 150 is obtained tea grounds saccharified liquid.
3. the preparation of fermentation medium：
Using above-mentioned tea grounds saccharified liquid as culture medium carbon source, the tryptone of the yeast extract and 0.5 wt% of adding 0.8 wt% is made
It is nitrogen source and the NaH of 0.3 wt%2PO4As phosphorus source；PH is to 5.0 for regulation, is configured to fermentation medium, is placed in triangular flask,
121 DEG C of 25 min of sterilizing, are cooled to standby after room temperature；Glucose oxidation and bacillus, rhizobium, Sarcina are accessed into liquid
Body seed culture medium prepares the seed liquor that bacteria cellulose produces bacterial strain, is then transferred to hair by the inoculum concentration of percent by volume 9%
Ferment culture medium, in 25 DEG C with 240 rpm rotating speeds under dynamic oscillation culture 1 day, then quiescent culture fermentation in 5 days is obtained bacterial fibers
Element.
Embodiment 4
1. zymotic fluid is prepared using tea grounds：
Tea grounds is chosen first and water presses solid-liquid mass ratio 1：10 are placed in juice extractor, it is smashed at a high speed and is stirred, and obtain
Tea grounds slurries, addition trace element, make its ultimate density be：0.2 g/L KH2PO4、0.6 g/L NaCl、0.3 g/L
MgSO4、0.4 mg/L ZnSO4、0.03 g/L FeCl3Regulation pH to 5.5, obtains the fermented and cultured with tea grounds juice as principal component
Base, is fitted into triangular flask, and sterilize 30 min at 121 DEG C, is cooled to room temperature standby.
2. the preparation of tea grounds saccharified liquid：
By bacillus（Bacillus）, hot rod Pseudomonas（Caldibacillus）, Cellvibrio（Cellvlbrio）As
Cellulose-degrading bacteria, the cellulose-degrading bacteria that culture is amplified through two-stage is accessed the triangular flask matched somebody with somebody honest material and sterilized and cooled down
In, in 40 DEG C of temperature, saccharification 48h under the fermentation condition of shaking speed 150rpm is obtained tea grounds saccharified liquid.
3. the preparation of fermentation medium：
Using above-mentioned tea grounds saccharified liquid as culture medium carbon source, the corn pulp of 20 wt% is added as nitrogen source and 0.4 wt%
KH2PO4As phosphorus source；PH is to 6.0 for regulation, is configured to fermentation medium, is placed in triangular flask, 121 DEG C of 30 min of sterilizing, cooling
It is standby after to room temperature.
4. fermentation prepares bacteria cellulose：
Acetic acid Pseudomonas, gluconate pyracetobacillus are accessed into liquid seed culture medium and prepares the seed that bacteria cellulose produces bacterial strain
Liquid, is then transferred to fermentation medium by the inoculum concentration of percent by volume 12%, in 22 DEG C with 180 rpm rotating speeds under shaken cultivation
3 days, then the prepared bacteria cellulose of quiescent culture fermentation in 15 days.
Embodiment 5
1. zymotic fluid is prepared using tea grounds：
Tea grounds is chosen first and water presses solid-liquid mass ratio 1：10 are placed in juice extractor, it is smashed at a high speed and is stirred, and obtain
Tea grounds slurries, addition trace element, make its ultimate density be：0.9 g/L KH2PO4、1.2 g/L NaCl、0.6 mg/L
ZnSO4、0.05 g/L FeCl3, pH to 6.0 is adjusted, the fermentation medium with tea grounds juice as principal component is obtained, load triangular flask
In, sterilize 30 min at 121 DEG C, treats to stop heating after the completion of its sterilizing, it is naturally cooled to room temperature.
2. the preparation of tea grounds saccharified liquid：
By bacillus（Bacillus）, hot acid Pseudomonas（Acidothermus）, hot rod Pseudomonas（Caldibacillus）, it is false
Zygosaccharomyces（Pseudomonas）And Erwinia（Erwinia）As cellulose-degrading bacteria, the fibre of culture will be amplified through two-stage
During the plain degradation bacteria of dimension is accessed with honest material and the triangular flask that has sterilized and cooled down, in 45 DEG C of temperature, the hair of the rpm of shaking speed 120
The h of saccharification 36 under the conditions of ferment, is obtained tea grounds saccharified liquid.
3. the preparation of fermentation medium：
Using above-mentioned tea grounds saccharified liquid as culture medium carbon source, the brewer's wort nitrogen of 15 wt% is added as nitrogen source and 0.5 wt%
NaH2PO4As phosphorus source；PH is to 6.0 for regulation, is configured to fermentation medium, is placed in triangular flask, 121 DEG C of 30 min of sterilizing, cooling
It is standby after to room temperature.
4. fermentation prepares bacteria cellulose：
Agrobacterium, campylobacter jejuni, gluconate pyracetobacillus are accessed into liquid seed culture medium and prepares bacteria cellulose life
The seed liquor of bacterial strain is produced, then fermentation medium is transferred to by the inoculum concentration of percent by volume 15%, is turned with 210 rpm in 28 DEG C
The lower shaken cultivation of speed 2 days, then quiescent culture fermentation in 7 days is obtained bacteria cellulose.
Claims (10)
1. a kind of method for preparing bacteria cellulose as carbon source by the use of tea grounds, it is characterised in that it is comprised the following specific steps that：
（1）Zymotic fluid is prepared using tea grounds：According to solid-to-liquid ratio 1 after tea grounds is cleaned:10 mix with water and squeeze the juice, and tea is smashed to obtain at a high speed
Dreg slurry, adds inorganic salts, and regulation pH is 4.0-6.0, obtains the fermentation medium with tea grounds slurry as principal component, loads triangular flask
In, 121 DEG C sterilizing 20-30 min, naturally cool to 20-30 DEG C it is standby；
（2）The preparation of tea grounds saccharified liquid：In step（1）Cellulose-degrading bacteria is inoculated with the tea grounds fermentation medium of preparation, is carried out
Saccharification of cellulose is hydrolyzed, and obtains tea grounds saccharified liquid；Its concrete technology is：The cellulose-degrading bacteria of activated culture is accessed into step
（1）Saccharification is carried out in the triangular flask；
（3）The preparation of fermentation medium：Using above-mentioned tea grounds saccharified liquid as culture medium carbon source, nitrogen source and phosphorus source are added, adjust pH
It is 4.0-6.0, is configured to fermentation medium, be placed in triangular flask, sterilizes standby；
（4）Fermentation prepares bacteria cellulose：The seed liquor that bacteria cellulose produces bacterial strain is accessed, is obtained by fermentation in 3-25 days thin
Fungin.
2. a kind of method for preparing bacteria cellulose as carbon source by the use of tea grounds according to claim 1, it is characterised in that：
The step（1）In tea grounds be the tea grounds discarded in Tea Processing production process.
3. a kind of method for preparing bacteria cellulose as carbon source by the use of tea grounds according to claim 1, it is characterised in that：
The step（1）In inorganic salts be KH2PO4、K2HPO4、NaCl、MgSO4、ZnSO4、FeCl3In one or more.
4. a kind of method for preparing bacteria cellulose as carbon source by the use of tea grounds according to claim 1, it is characterised in that：
The step（2）In cellulose-degrading bacteria be Cytophage（Cytophaga）, bacillus（Bacillus）, hot acid
Pseudomonas（Acidothermus）, hot rod Pseudomonas（Caldibacillus）, Cellvibrio（Cellvlbrio）, pseudomonas
（Pseudomonas）And Erwinia（Erwinia）In one or more.
5. a kind of method for preparing bacteria cellulose as carbon source by the use of tea grounds according to claim 1, it is characterised in that：
The step（2）In saccharification condition be 30-45 DEG C, saccharification 36-120 h under conditions of rotating speed 120-180 rpm.
6. a kind of method for preparing bacteria cellulose as carbon source by the use of tea grounds according to claim 1, it is characterised in that：
The step（3）In tea grounds saccharified liquid add appropriate nitrogen source and phosphorus source first, adjust pH, be placed in triangular flask, 121 DEG C go out
Bacterium 20-30 min, after being cooled to room temperature, access the seed liquor that bacteria cellulose produces bacterial strain, are turned in 120-240 rpm using elder generation
The lower shaken cultivation of speed 1 ~ 3 day, then the quiescent culture fermentation form of being association of activity and inertia of 3 ~ 20 days is obtained bacteria cellulose.
7. a kind of method for being prepared bacteria cellulose as carbon source by the use of tea grounds according to claim 1 or 6, its feature is existed
In：The step（3）In nitrogen source for 0.1-1.0 wt% yeast extract and 0.1-0.5 wt% tryptone；Or be
The ammonium sulfate or corn pulp or brewer's wort of 0.1-2.0 wt%；The step（3）In phosphorus source for 0.1-1.0 wt% KH2PO4、
K2HPO4Or NaH2PO4Or Na2HPO4。
8. a kind of method for preparing bacteria cellulose as carbon source by the use of tea grounds according to claim 1, it is characterised in that：
The step（4）20-30 DEG C of middle fermentation condition, dynamic oscillation culture 1-3 days under 120-240 rpm rotating speeds, then quiescent culture
3-20 days.
9. a kind of method for preparing bacteria cellulose as carbon source by the use of tea grounds according to claim 1, it is characterised in that：
The step（4）In bacteria cellulose production bacterial strain be acetic acid Pseudomonas, gluconobacter suboxydans category, gluconic acid Acetobacter, Portugal
The glycoxidative bacillus of grape, rhizobium, Sarcina, pseudomonas, achromobacter, Alcaligenes, Aerobacter, fixed nitrogen
One or more in Pseudomonas, Agrobacterium, onion pseudomonas sp, campylobacter jejuni, gluconate pyracetobacillus.
10. a kind of method for being prepared bacteria cellulose as carbon source by the use of tea grounds according to claim 1, its feature is existed
In：The step（4）The inoculum concentration of middle bacteria cellulose production bacterial strain is 3-15%vol.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108315371A (en) * | 2018-04-20 | 2018-07-24 | 北京理工大学珠海学院 | A kind of cultural method of bacteria cellulose |
| CN109652489A (en) * | 2018-12-21 | 2019-04-19 | 华南协同创新研究院 | A kind of method of the full fermentation bacteria cellulose film of One-step production class containing mushroom extractive from fermentative |
| CN109652489B (en) * | 2018-12-21 | 2020-12-22 | 华南协同创新研究院 | Method for producing full-fermentation bacterial cellulose membrane containing mushroom fermentation extract by one-step method |
| CN113481256A (en) * | 2021-08-10 | 2021-10-08 | 武汉纺织大学 | Method for producing bacterial cellulose by jasmine flower culture |
| CN114561437A (en) * | 2022-03-24 | 2022-05-31 | 中国科学院广州能源研究所 | Method for preparing functional bacterial cellulose by in-situ fermentation of eucommia ulmoides hydrolysate |
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