CN100365128C - Method for preparing bacteria cellulose - Google Patents
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- CN100365128C CN100365128C CNB2006100332958A CN200610033295A CN100365128C CN 100365128 C CN100365128 C CN 100365128C CN B2006100332958 A CNB2006100332958 A CN B2006100332958A CN 200610033295 A CN200610033295 A CN 200610033295A CN 100365128 C CN100365128 C CN 100365128C
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- 238000000034 method Methods 0.000 title claims abstract description 13
- 229920002678 cellulose Polymers 0.000 title claims description 40
- 239000001913 cellulose Substances 0.000 title claims description 40
- 241000894006 Bacteria Species 0.000 title claims description 39
- 238000000855 fermentation Methods 0.000 claims abstract description 47
- 230000004151 fermentation Effects 0.000 claims abstract description 47
- 244000235858 Acetobacter xylinum Species 0.000 claims abstract description 29
- 235000002837 Acetobacter xylinum Nutrition 0.000 claims abstract description 29
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 27
- 230000001954 sterilising effect Effects 0.000 claims abstract description 21
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 20
- 229920002749 Bacterial cellulose Polymers 0.000 claims abstract description 19
- 239000005016 bacterial cellulose Substances 0.000 claims abstract description 19
- 239000002994 raw material Substances 0.000 claims abstract description 19
- 230000003068 static effect Effects 0.000 claims abstract description 11
- 239000012452 mother liquor Substances 0.000 claims description 28
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 18
- PTHCMJGKKRQCBF-UHFFFAOYSA-N Cellulose, microcrystalline Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC)C(CO)O1 PTHCMJGKKRQCBF-UHFFFAOYSA-N 0.000 claims description 16
- 238000011081 inoculation Methods 0.000 claims description 16
- 239000002245 particle Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 11
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 10
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 10
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 10
- 239000002054 inoculum Substances 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 9
- 238000010438 heat treatment Methods 0.000 claims description 9
- 238000009835 boiling Methods 0.000 claims description 8
- 229940041514 candida albicans extract Drugs 0.000 claims description 8
- 239000012138 yeast extract Substances 0.000 claims description 8
- 150000001720 carbohydrates Chemical class 0.000 claims description 6
- 235000014633 carbohydrates Nutrition 0.000 claims description 6
- 235000015203 fruit juice Nutrition 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 6
- 235000020413 lychee juice Nutrition 0.000 claims description 5
- 235000013379 molasses Nutrition 0.000 claims description 5
- 229910019142 PO4 Inorganic materials 0.000 claims description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- 235000020378 longan juice Nutrition 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
- 239000010452 phosphate Substances 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- 239000011591 potassium Substances 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 235000009392 Vitis Nutrition 0.000 claims description 2
- 241000219095 Vitis Species 0.000 claims description 2
- 230000002070 germicidal effect Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract 3
- 230000036782 biological activation Effects 0.000 abstract 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 24
- 238000004140 cleaning Methods 0.000 description 12
- 235000013339 cereals Nutrition 0.000 description 8
- 239000012467 final product Substances 0.000 description 7
- 238000012856 packing Methods 0.000 description 7
- 241000589220 Acetobacter Species 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- 230000003750 conditioning effect Effects 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 4
- 230000004913 activation Effects 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 235000006180 nutrition needs Nutrition 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 241000877401 Saccharomyces ellipsoideus Species 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 108010025899 gelatin film Proteins 0.000 description 1
- 235000002532 grape seed extract Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention discloses a method for preparing bacterial cellulose by a biological activation method. In the method, after the first sterilization treatment, fermentation mother liquid is inoculated with yeast for fermentation; ultrahigh-temperature instant sterilization is carried out for the second sterilization of the fermentation mother liquid; the sterilized and cooled fermentation mother liquid is inoculated into bacillus xylinus; then block gel bacterial cellulose can be obtained by aerobic culture and static culture. The present invention can effectively improve the utilization rate of raw materials and accelerate the synthesizing speed of the bacterial cellulose.
Description
Technical field
The invention belongs to technical field of biological fermentation, particularly a kind of preparation method of bacteria cellulose.
Background technology
Bacteria cellulose is one of emphasis biomaterial of studying both at home and abroad at present, its main characteristic is ultrapure (100% Mierocrystalline cellulose), ultra-fine (nano level), superpower (high Young's modulus) and high water absorbing and retaining rate (more than 1: 50), high chemically derived activity, do not contain xylogen, hemicellulose, its extraction process is simple, the extraction product purity is high, is considered to best in the world Mierocrystalline cellulose at present.Bacteria cellulose is with a wide range of applications in industries such as medicine, food, chemical industry, electronics.At present, the main bacterial strain that is used to study and produce bacteria cellulose in the world wide is acetobacter xylinum (Acetobacter Xylimum), and the field of research is that the separation screening of the synthesis mechanism of bacteria cellulose, superior strain is cultivated and aspect such as cellulosic application.Because the nutritional needs of microorganism mechanism more complicated, understood at present the basic nutrition requirement of acetobacter xylinum, but also do not understand for its key factor of breeding fast, existing technology mainly adopts the method for traditional composite substratum to come to provide nutrition for acetobacter xylinum, and the present invention adopts the method for bioactivation substratum, by saccharomycetic effect, make the composition of substratum obtain again improvement, effectively accelerate acetobacter xylinum and produced cellulosic speed, and improved utilization ratio of raw materials.
Summary of the invention
In order to solve above-mentioned the deficiencies in the prior art part, the object of the present invention is to provide a kind of method of utilizing bioactivation legal system detailed information fungin.This method can effectively improve utilization ratio of raw materials, accelerate the resultant velocity of bacteria cellulose.
The present invention is directed to present fermentation technique, improve the active effective constituent of its substratum, and kill the pollution of activation yeast, the assorted bacterium of control, and effectively keep its activeconstituents by the high-temperature short-time sterilization technology, accelerate bacterium synthetic speed to reach, improve the purpose of raw material availability.
Purpose of the present invention is achieved through the following technical solutions: the preparation method of described bacteria cellulose comprises the steps:
After the fermentation mother liquor process germicidal treatment first time, the inoculation yeast bacterium, the yeast inoculum size is 0.3～0.5%, ferment, leavening temperature keeps 28～32 ℃, cultivate after 36～72 hours, adopt Ultra High Temperature Short Time that fermentation mother liquor is carried out re-sterilise, sterilize and insert acetobacter xylinum (Acetobacter Xylimum) in the cooled fermentation mother liquor, the acetobacter xylinum inoculum size is 5～10%, behind the aerated culture 24～36 hours, static cultivation 50～120 hours can make the bacterial cellulose gel sheet.
After the above-mentioned bacterial cellulose gel sheet that makes is cut into particle, the NaOH solution (weight percent meter) of cellulose grain and 1～5% is mixed by 1: 1～2 mass ratio, be heated to 60～80 ℃, handled 1～4 hour, filter, clean up, can obtain the cellulose grain of gel state.
Described fermentation mother liquor is mainly formed (by weight percentage) by 3～20% carbohydrate raw material, 0.1～1.0% nitrogen-containing material, and above-mentioned raw materials is added the water heating for dissolving, and the pH value is adjusted to 2.8～4.5, after the filtration promptly.Wherein the carbohydrate raw material comprises molasses, fruit juice or white sugar etc., and molasses or fruit juice account for 50～75% of carbohydrate raw material total mass, and nitrogen-containing material comprises ammonium sulfate or yeast extract paste etc., and ammonium sulfate accounts for 85～95% of nitrogen-containing material.Described fruit juice comprises Lychee juice, longan juice or Sucus Vitis viniferae etc.
Described pH value adopts adjustings such as acetic acid, citric acid or potassium primary phosphate.
The sterilization first time of described fermentation mother liquor is adopted and is boiled mode, boiling time: got final product in 5～10 minutes.
The condition of Ultra High Temperature Short Time is: temperature is 125～135 ℃, and the time is 20～30 seconds.
The present invention utilizes the bioactivation method to produce bacteria cellulose: it produces the nutritional needs configuration fermentation mother liquor of bacteria cellulose according to acetobacter xylinum, fermentation mother liquor is through after the heating, filtration, sterilization, make fermentation mother liquor activation, Ultra High Temperature Short Time then by carrying out the inoculation yeast bacterium, cool off, inoculate acetobacter xylinum, cultivation, packing, static cultivation, results, aftertreatment, pack product.
The present invention compared with prior art has the following advantages and beneficial effect:
By inoculation yeast bacterium in fermentation mother liquor, the activation fermentation mother liquor makes it to produce the acetobacter xylinum needed somatomedin of growing; Kill yeast and other bacteriums by the ultra high temperature short time sterilization technology, effectively keep the chemotrophy composition of fermentation mother liquor simultaneously; Can effectively improve the resultant velocity of utilization ratio of raw materials, quickening bacteria cellulose.The present invention makes the yield of bacterial fibers bring up to 32g/L from 25g/L; Advanceed to 36～48 hour from 48～72 hours the start time of static fermentation Mierocrystalline cellulose synthetic fast growing period; The generated time of the bacterial fibers gel film of 12 millimeters thickness has shortened 24 hours at least; The infection rate of fiber gel sheet drops to below 1%; The comprehensive yield of product is greater than 85%.
Description of drawings
Fig. 1 is a process flow sheet of the present invention.
Embodiment
The present invention is described in further detail below in conjunction with embodiment and accompanying drawing, but embodiments of the present invention are not limited thereto.
Embodiment 1
As shown in Figure 1, the preparation method of bacteria cellulose of the present invention comprises the steps:
50 kilograms of molasses of raw material, 50 kilograms of white sugar, 3 kilograms of ammonium sulfate, 200 gram yeast extract pastes, add after the water heating for dissolving and and be adjusted to 4.5, calmly heavily to 1000 kilograms with pH value conditioning agent acetic acid; Promptly get fermentation mother liquor after the filtration, fermentation mother liquor heated and boiled (boiling time: 8 minutes) is sterilization, be cooled to 40 ℃ and get final product inoculation yeast, the inoculation active dry yeast is (commercially available, dried Saccharomyces Cerevisiae in S accharomyces cerevisiac) 5 kilogram, cultivate Ultra High Temperature Short Time after 36 hours for 28～32 ℃, the condition of sterilization is under 135 ℃ of temperature, 20 times in second; Be cooled to 40 ℃ and can inoculate acetobacter xylinum (acetobacter xylinum 1.1812, purchase institute of microbiology) in the Chinese Academy of Sciences, the acetobacter xylinum inoculum size is 5%, behind the aerated culture 30 hours, divide to install in the air dried fermentation dish (fermentation dish after cleaning up, heat kill bacterium), thickness is 6 millimeters, after the stacking static cultivation 50～60 hours, can gather in the crops the block bacteria cellulose (bacterial cellulose gel sheet) of gel, about 5 millimeters of its thickness.After the bacterial cellulose gel sheet is cut into particle, the NaOH solution (by weight percentage) of above-mentioned particle and 1% is compared uniform mixing by 1: 1 quality, be heated to 80 ℃, handled 1 hour, filter out moisture content, clean 3～5 times to cleaning up with clear water again, can obtain the cellulose grain of gel state, packing.
Embodiment 2
100 kilograms of Lychee juices of raw material, 50 kilograms of white sugar, 5 kilograms of ammonium sulfate, 200 gram yeast extract pastes, add after the water heating for dissolving and and be adjusted to 2.8, calmly heavily to 1000 kilograms with pH value conditioning agent acetic acid; Promptly get fermentation mother liquor after the filtration, fermentation mother liquor heated and boiled (boiling time: 10 minutes) is sterilization, be cooled to 30 ℃ and get final product inoculation yeast, 3 kilograms of active dry yeasts of inoculation are (commercially available, wine yeast Saccharomycesellipsoideus), cultivate Ultra High Temperature Short Time after 36 hours for 28～32 ℃, the condition of sterilization is under 125 ℃ of temperature, 30 times in second; Be cooled to 30 ℃ and can inoculate acetobacter xylinum (Acetobacter Xylimum, acetobacter xylinum 1.1812, purchase institute of microbiology) in the Chinese Academy of Sciences, the acetobacter xylinum inoculum size is 10%, and aerated culture is after 24 hours, and branch installs to the fermentation dish, and (the fermentation dish is after cleaning up, the heat kill bacterium) in, thickness is 15 millimeters, and the stacking static cultivation can be gathered in the crops the block bacteria cellulose (bacterial cellulose gel sheet) of gel after 110～120 hours.After the bacterial cellulose gel sheet is cut into particle, the NaOH solution (by weight percentage) of above-mentioned particle and 5% is compared uniform mixing by 1: 1.5 quality, be heated to 70 ℃, handled 2 hours, filter out moisture content, clean 3～5 times to cleaning up with clear water again, can obtain the cellulose grain of gel state, packing, 34 kilograms of product Mierocrystalline cellulose dry weights.
Embodiment 3
100 kilograms of Sucus Vitis viniferaes of raw material, 50 kilograms of white sugar, 5 kilograms of ammonium sulfate, 260 gram yeast extract pastes, add after the water heating for dissolving and and be adjusted to 3.5, calmly heavily to 1000 kilograms with pH value conditioning agent acetic acid; Promptly get fermentation mother liquor after the filtration, fermentation mother liquor heated and boiled (boiling time: 10 minutes) is sterilization, be cooled to 30 ℃ and get final product inoculation yeast, 5 kilograms of active dry yeasts of inoculation are (commercially available, bread yeast Saccharomycescerevisiac), cultivate Ultra High Temperature Short Time after 36 hours for 28～32 ℃, the condition of sterilization is under 130 ℃ of temperature, 25 times in second; Be cooled to 30 ℃ and can inoculate acetobacter xylinum (Acetobacter Xylimum, acetobacter xylinum 1.1812, purchase institute of microbiology) in the Chinese Academy of Sciences, the acetobacter xylinum inoculum size is 8%, behind the aerated culture 36 hours, branch installs to the fermentation dish, and (the fermentation dish is after cleaning up, the heat kill bacterium) in, thickness is 15 millimeters, and the stacking static cultivation is after 100～110 hours, can gather in the crops the block bacteria cellulose (bacterial cellulose gel sheet) of gel, after the bacterial cellulose gel sheet is cut into particle, with the NaOH solution (by weight percentage) of above-mentioned particle and 3% by 1: 2 quality than uniform mixing, be heated to 60 ℃, handled 4 hours, filter out moisture content, clean 3～5 times to cleaning up with clear water again, can obtain the cellulose grain of gel state, packing gets 36 kilograms of Mierocrystalline cellulose dry weights.
Embodiment 4
20 kilograms of longan juices of raw material, 10 kilograms of white sugar, 1 kilogram of ammonium sulfate, 100 gram yeast extract pastes add after the water heating for dissolving and with pH value conditioning agent (acetic acid, potassium primary phosphate) and are adjusted to 2.8, and are fixed heavily to 1000 kilograms; Promptly get fermentation mother liquor after the filtration, fermentation mother liquor heated and boiled (boiling time: 5 minutes) is sterilization, be cooled to 30 ℃ and get final product inoculation yeast, the inoculation active dry yeast is (commercially available, dried Saccharomyces Cerevisiae in S accharomycescerevisiac) 4 kilogram, cultivate Ultra High Temperature Short Time after 36 hours for 28～32 ℃, the condition of sterilization is under 135 ℃ of temperature, 20 times in second; Be cooled to 30 ℃ and can inoculate acetobacter xylinum (AcetobacterXylimum), the acetobacter xylinum inoculum size is 5%, behind the aerated culture 30 hours, branch installs to air dried fermentation dish, and (the fermentation dish is after cleaning up, the heat kill bacterium) in, thickness is 6 millimeters, and the stacking static cultivation is after 50～60 hours, can gather in the crops the block bacteria cellulose (bacterial cellulose gel sheet) of gel, about 5 millimeters of its thickness.After the bacterial cellulose gel sheet is cut into particle, the NaOH solution (by weight percentage) of above-mentioned particle and 1% is compared uniform mixing by 1: 1 quality, be heated to 80 ℃, handled 1 hour, filter out moisture content, clean 3～5 times to cleaning up with clear water again, can obtain the cellulose grain of gel state, packing.
Embodiment 5
100 kilograms of Lychee juices of raw material, 100 kilograms of white sugar, 9 kilograms of ammonium sulfate, 1000 gram yeast extract pastes, add after the water heating for dissolving and and be adjusted to 4.0, calmly heavily to 1000 kilograms with pH value conditioning agent citric acid; Promptly get fermentation mother liquor after the filtration, fermentation mother liquor heated and boiled (boiling time: 8 minutes) is sterilization, be cooled to 32 ℃ and get final product inoculation yeast, 5 kilograms of active dry yeasts of inoculation are (commercially available, bread yeast Saccharomycescerevisiac), cultivate Ultra High Temperature Short Time after 36 hours for 28～32 ℃, the condition of sterilization is under 130 ℃ of temperature, 25 times in second; Be cooled to 32 ℃ and can inoculate acetobacter xylinum (Acetobacter Xylimum, acetobacter xylinum 1.1812, purchase institute of microbiology) in the Chinese Academy of Sciences, the acetobacter xylinum inoculum size is 8%, behind the aerated culture 36 hours, branch installs to the fermentation dish, and (the fermentation dish is after cleaning up, the heat kill bacterium) in, thickness is 15 millimeters, and the stacking static cultivation can be gathered in the crops the block bacteria cellulose (bacterial cellulose gel sheet) of gel after 100～110 hours, after the bacterial cellulose gel sheet is cut into particle, with the NaOH solution (by weight percentage) of above-mentioned particle and 3% by 1: 1 quality than uniform mixing, be heated to 60 ℃, handled 4 hours, filter out moisture content, clean 3～5 times to cleaning up with clear water again, can obtain the cellulose grain of gel state, packing.
Embodiment 6
30 kilograms of Lychee juices of raw material, 10 kilograms of white sugar, 1.5 kilograms of ammonium sulfate, 260 gram yeast extract pastes, add after the water heating for dissolving and and be adjusted to 4.5, calmly heavily to 1000 kilograms with pH value conditioning agent citric acid; Promptly get fermentation mother liquor after the filtration, fermentation mother liquor heated and boiled (boiling time: 6 minutes) is sterilization, be cooled to 28 ℃ and get final product inoculation yeast, 5 kilograms of active dry yeasts of inoculation are (commercially available, bread yeast Saccharomycescerevisiac), cultivate Ultra High Temperature Short Time after 36 hours for 28～32 ℃, the condition of sterilization is under 130 ℃ of temperature, 25 times in second; Be cooled to 28 ℃ and can inoculate acetobacter xylinum (Acetobacter Xylimum, acetobacter xylinum 1.1812, purchase institute of microbiology in the Chinese Academy of Sciences)), the acetobacter xylinum inoculum size is 8%, behind the aerated culture 36 hours, branch installs to the fermentation dish, and (the fermentation dish is after cleaning up, the heat kill bacterium) in, thickness is 15 millimeters, and the stacking static cultivation can be gathered in the crops the block bacteria cellulose (bacterial cellulose gel sheet) of gel after 90～100 hours, after the bacterial cellulose gel sheet is cut into particle, with the NaOH solution (by weight percentage) of above-mentioned particle and 1% by 1: 2 quality than uniform mixing, be heated to 70 ℃, handled 4 hours, filter out moisture content, clean 3～5 times to cleaning up with clear water again, can obtain the cellulose grain of gel state, packing.
Claims (7)
1. the preparation method of a bacteria cellulose is characterized in that comprising the steps:
After the fermentation mother liquor process germicidal treatment first time, the inoculation yeast bacterium, the yeast inoculum size is 0.3～0.5%, ferments, leavening temperature keeps 28～32 ℃, cultivate after 36～72 hours, adopt Ultra High Temperature Short Time that fermentation mother liquor is carried out re-sterilise, insert acetobacter xylinum in the cooled fermentation mother liquor of sterilizing, the acetobacter xylinum inoculum size is 5～10%, behind the aerated culture 24～36 hours, static cultivation 50～120 hours promptly makes the bacterial cellulose gel sheet; Described fermentation mother liquor mainly is made up of 3～20% carbohydrate raw material, 0.1～1.0% nitrogen-containing material, and above-mentioned raw materials is added the water heating for dissolving, and the pH value is adjusted to 2.8～4.5, after the filtration promptly.
2. the preparation method of bacteria cellulose according to claim 1, it is characterized in that: after described bacterial cellulose gel sheet is cut into particle, the NaOH solution of particle and 1～5% is mixed by 1: 1～2 mass ratio, be heated to 60～80 ℃, handled 1～4 hour, filter, clean up, promptly obtain the cellulose grain of gel state.
3. the preparation method of bacteria cellulose according to claim 1, it is characterized in that: described carbohydrate raw material is molasses+white sugar or fruit juice+white sugar, molasses or fruit juice account for 50～75% of carbohydrate raw material total mass, nitrogen-containing material is ammonium sulfate+yeast extract paste, and ammonium sulfate accounts for 85～95% of nitrogen-containing material.
4. the preparation method of bacteria cellulose according to claim 3, it is characterized in that: described fruit juice comprises Lychee juice, longan juice or Sucus Vitis viniferae.
5. the preparation method of bacteria cellulose according to claim 1 is characterized in that: described pH value employing acetic acid, citric acid or potassium primary phosphate adjusting.
6. the preparation method of bacteria cellulose according to claim 1 is characterized in that: the sterilization first time of described fermentation mother liquor is adopted and is boiled mode, and boiling time is 5～10 minutes.
7. the preparation method of bacteria cellulose according to claim 1, it is characterized in that: the condition of Ultra High Temperature Short Time is: temperature is 125～135 ℃, the time is 20～30 seconds.
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| CNB2006100332958A CN100365128C (en) | 2006-01-24 | 2006-01-24 | Method for preparing bacteria cellulose |
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