CN106888620A - A kind of raising old kind of method of survival rate of seedling of agate card - Google Patents

A kind of raising old kind of method of survival rate of seedling of agate card Download PDF

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CN106888620A
CN106888620A CN201710118688.7A CN201710118688A CN106888620A CN 106888620 A CN106888620 A CN 106888620A CN 201710118688 A CN201710118688 A CN 201710118688A CN 106888620 A CN106888620 A CN 106888620A
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agate card
old kind
ion beam
old
agate
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张瑞明
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A01N59/20—Copper

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  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Soil Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses a kind of raising old kind of method of survival rate of seedling of agate card, step is as follows：（1）Old kind of sterilizing of agate card；（2）Old kind of activating agent immersion treatment of agate card；（3）N+The old kind of ion beam mutation agate card；（4）The old kind of low temperature drying of agate card is preserved, nursery after ultrasonication.The present invention passes through chemicals treatment, N+The cooperative effect of ion beam mutation and ultrasonication, substantially increases the viability and emergence rate of the old kind of agate card, and protection and utilization, agate the card production and the raising of economic benefit to agate card germ plasm resource are respectively provided with significance.

Description

A kind of raising old kind of method of survival rate of seedling of agate card
Technical field
The present invention relates to agate card cultivation technical field, specifically a kind of old kind of side of survival rate of seedling of raising agate card Method.
Background technology
Agate card is a kind of herbaceous plant for originating in South America Andes High aititude mountain area, belongs to Cruciferae Lepidium apetalum Category, has longer cultivation and edible history in andes region, and the height above sea level of its distribution is 2500-4450 meters, is high The Major Foods of cold mountain area American Indian.Tamed by Yin Jia American Indians before agate card 1300-2000, in the life of American Indian In tradition, agate card is the sexual desire for being used to strengthen people and livestock as a kind of aphrodisiac, improves the fecundity of women and dam.
Agate card subterranean stem is nutritious, rich in carbohydrate, protein and unrighted acid.It contains 59% carbon Hydrate, the protein of 10%-14%, 9% cellulose and 2% lipoid substance, especially contain substantial amounts of primary amino acid With aliphatic acid and the mineral nutrient element of high content（Such as iodine, iron, potassium and calcium）.Additionally, also contain multivitamin, such as VB1, VB2, VB12, VE and VC.Modern medicine study shows that it can improve the sexual desire 180%-200% of male subject, and sperm is produced Raw amount increases nearly 1 times.It has the effect for reducing blood pressure, and heart is free from side effects.Therefore, agate card is referred to as natural " vigour ", " hormone engine ".Agate card has regulation hormone secretion, promotes immune and metabolic function, is favorably improved memory Power, antidepression, in treatment chronic fatigue syndrome, sex dysfunction, pulmonary tuberculosis, anaemia, leukaemia, AIDS, cancer（It is special It is not stomach cancer）, fat-reducing and excessive drinking etc. have certain effect in illness.Agate card also has significant anti-senescence function, and it is to the elderly Spirit and muscle power have obvious facilitation.With health of people health care consciousness raising and food-safe concern, with Agate card is promoted rapidly for all kinds of health products of main material production on the international markets such as America and Europe, Japan, obtains more and more disappearing The favor of the person of expense.After the nineties in 20th century, FAO (Food and Agriculture Organization of the United Nation) starts to push greatly using agate card as international important nutraceutical Extensively.Maca powder in 2011 is approved as new resource food by No. 13 bulletin of the Ministry of Public Health of China, has thus opened agate and has been stuck in China New page.At present, in China's introducing and planting success, Cultivated In Yunnan agate card yield accounts for the 80% of national yield to agate card, as agate It is stuck in the main product ground of China.In recent years, national cultivated area persistently rises, and the ground such as Tibet, Xinjiang, Qinghai, Sichuan is also successively Launch plantation.
Agate card is main with seminal propagation, and the seed for harvesting then after planting has germination percentage higher, is stored up through every 1 year Seed is aged caused by various factors behind Tibetan.Old kind of viability of agate card declines, emergence rate is low, seedling is weak, slow-growing, premunition Difference, certain influence is caused to agate card introducing and planting, and agate card type is expensive, also brings along larger economic loss.Cause How this, improve the vigor of the old kind of agate card, and protection and utilization, agate the card production and the raising of economic benefit to germ plasm resource have Significance.
The content of the invention
The purpose of the present invention is directed to the old kind of viability decline of existing agate card, emergence rate is low, seedling is weak, slow-growing, anti- The defect of sick power difference, there is provided one kind passes through chemicals treatment, N+The cooperative effect of ion beam mutation and ultrasonication, so that greatly The big method that improve old kind of viability of agate card and emergence rate.
The purpose of the present invention is solved by the following technical programs：
A kind of raising old kind of method of survival rate of seedling of agate card, it is characterised in that：Old kind of survival rate of seedling side of the raising agate card The step of method is：
（1）Old kind of sterilizing of agate card：To be put into culture dish after the pending old kind cleaning of agate card, added in culture dish 0.6% liquor natrii hypochloritis, sterilized 15~20min, and ultrapure water is used 3~5 times after sterilizing；
（2）Old kind of activating agent immersion treatment of agate card：The old kind of agate card is soaked into first 2~4h of immersion in lower concentrations of active agent, then Seed is taken out, is dried, sprayed the activating agent of high concentration with watering can and stirring agate card type makes seed uniformly soak, dried in the air again It is dry, repeatedly by baked seed after 2~3 times；
（3）N+The old kind of ion beam mutation agate card：Agate card seed embryo portion is put into sample disc, ion note towards ion beam mutation direction Start preheating 2h before entering, under vacuo with energy as 35KeV, line size 10mA, each burst length as 5s, target chamber vacuum It is 6.0 × 10-4~1.3 × 10-3Pa carries out N+Ion beam mutation；
（4）The old kind of low temperature drying of agate card is preserved, nursery after ultrasonication：By the old kind of low temperature drying of agate card after ion beam mutation Preserve, with 20% polyglycol solution seed soaking 20h before nursery, then with 55~65kHz ultrasonication 10min, then take out, Seeding and seedling raising after 2~3d of presoaking and germinating.
The step（2）Middle lower concentrations of active agent component and proportioning are：ZnSO420~25g/L, CuSO4·5H2O 10~ 12g/L, MnCl26~8g/L；Concentration of component is 12~15 times of lower concentrations of active agent in the high concentration activating agent.
The step（3）Middle N+Ion beam mutation dosage is 0.9 × 1037ion/cm2~1.3 × 1037ion/cm2。
The step（4）Middle low temperature drying is preserved and refers to temperature less than 5 DEG C, and humidity is less than 45%.
The present invention has the following advantages compared to existing technology：
Although having N at present+The research that ion beam mutation and ultrasonication vegetable seeds can improve bud ratio is reported, but N+From Beamlet injects very limited to the facilitation effect that seed is sprouted with ultrasonication, and practical value is relatively low.The invention The method for applying ion beam mutation and ultrasonication after being processed using activating agent again, chemicals treatment, N+Ion beam mutation and super Occurs significant cooperative effect after sonicated, so as to substantially increase the viability and emergence rate of the old kind of agate card, to agate card type Protection and utilization, the production of agate card and the raising of economic benefit of matter resource are respectively provided with significance.
Specific embodiment
With reference to embodiment, the present invention is further illustrated.
A kind of raising old kind of method of survival rate of seedling of agate card, steps of the method are：
（1）Old kind of sterilizing of agate card：To be put into culture dish after the pending old kind cleaning of agate card, added in culture dish 0.6% liquor natrii hypochloritis, sterilized 15~20min, and ultrapure water is used 3~5 times after sterilizing；
（2）Old kind of activating agent immersion treatment of agate card：The old kind of agate card is soaked first in lower concentrations of active agent（Lower concentrations of active agent component And proportioning is：ZnSO420~25g/L, CuSO4·5H2O 10~12g/L, MnCl26~8g/L）2~4h of middle immersion, then Seed is taken out, is dried, the activating agent of high concentration is sprayed with watering can（Concentration of component is lower concentrations of active agent in high concentration activating agent 12~15 times）And stirring agate card type makes seed uniformly soak, and dries again, repeatedly by baked seed after 2~3 times；
（3）N+The old kind of ion beam mutation agate card：Agate card seed embryo portion is put into sample disc, ion note towards ion beam mutation direction Start preheating 2h before entering, under vacuo with energy as 35KeV, line size 10mA, each burst length as 5s, target chamber vacuum It is 6.0 × 10-4~1.3 × 10-3Pa carries out N+Ion beam mutation, N+Ion beam mutation dosage is 0.9 × 1037ion/cm2~1.3 ×1037ion/cm2；
（4）The old kind of low temperature drying of agate card is preserved, nursery after ultrasonication：The old kind of agate card after ion beam mutation is placed in temperature Less than being preserved under 5 DEG C, low temperature drying environment of the humidity less than 45%, with the seed soaking of 20% polyglycol solution 20h, Ran Houyong before nursery 55~65kHz ultrasonication 10min, then take out, seeding and seedling raising after 2~3d of presoaking and germinating.
Embodiment
First, different disposal method is to the old kind of influence of survival rate of seedling of agate card
The new maturation for harvesting sufficiently agate card type is purchased within 2012 from the market, germination percentage test is carried out then, obtain novel species Germination percentage is 89.4%.Temperature is subsequently placed in less than being preserved under 5 DEG C, low temperature drying environment of the humidity less than 45%, is taken within 2 months 2016 Going out carries out germination test, and the average germination percentage of agate card Chen Zhong is 26.4%, it can be found that prolonged storage has had a strong impact on The bud ratio of agate card type.
The old kind of agate card is taken, is divided into 8 groups, every group 200, being respectively adopted before nursery carries out different disposal method, then detects Old kind of germination percentage change.The corresponding processing method of different grouping is as follows：
1st group uses integrated conduct method of the invention, specially：（1）Old kind of sterilizing of agate card：Pending agate card is old It is put into culture dish after kind of cleaning, adds 0.6% liquor natrii hypochloritis in culture dish, sterilize 17.5min, with ultrapure after sterilizing Water is rinsed 4 times；（2）Old kind of activating agent immersion treatment of agate card：The old kind of agate card is soaked first in lower concentrations of active agent（Lower concentrations of active Agent component and proportioning are：ZnSO422.5g/L, CuSO4·5H2O 11g/L, MnCl27g/L）Middle immersion 3h, then by seed Take out, dry, the activating agent of high concentration is sprayed with watering can（Concentration of component is the 13.5 of lower concentrations of active agent in high concentration activating agent Times）And stirring agate card type makes seed uniformly soak, and dries again, repeatedly by baked seed after 3 times；（3）N+Ion beam mutation The old kind of agate card：Agate card seed embryo portion is put into sample disc towards ion beam mutation direction, start shooting preheating 2h before ion implanting, true Under sky with energy as 35KeV, line size 10mA, each burst length as 5s, target chamber vacuum be 0.9 × 10-4~1.05 × 10-3Pa carries out N+Ion beam mutation（In processing procedure, vacuum degree in vacuum chamber occurs faint change）, N+Ion beam mutation dosage It is 1.1 × 1037ion/cm2；（4）The old kind of low temperature drying of agate card is preserved, nursery after ultrasonication：By the agate after ion beam mutation Block old kind and be placed in temperature less than being preserved under 5 DEG C, low temperature drying environment of the humidity less than 45%, 20% polyglycol solution is used before nursery Seed soaking 20h, then with 60kHz ultrasonication 10min, then takes out, seeding and seedling raising after presoaking and germinating 2d, surveys germination percentage；
2nd group only with chemicals treatment, specially：（1）Old kind of sterilizing of agate card：To be put after the pending old kind cleaning of agate card Enter in culture dish, 0.6% liquor natrii hypochloritis is added in culture dish, sterilize 17.5min, ultrapure water is used 4 times after sterilizing； （2）Old kind of activating agent immersion treatment of agate card：The old kind of agate card is soaked first in lower concentrations of active agent（Lower concentrations of active agent component and match somebody with somebody Than for：ZnSO422.5g/L, CuSO4·5H2O 11g/L, MnCl27g/L）Middle immersion 3h, then takes out seed, dries, The activating agent of high concentration is sprayed with watering can（Concentration of component is 13.5 times of lower concentrations of active agent in high concentration activating agent）And stir Agate card type makes seed uniformly soak, and dries again, repeatedly by nursery after baked seed after 3 times, surveys germination percentage；
3rd group only with N+Ion beam mutation treatment, specially：（1）Old kind of sterilizing of agate card：By the pending old kind of agate card It is put into culture dish after cleaning, 0.6% liquor natrii hypochloritis is added in culture dish, is sterilized 17.5min, and ultra-pure water is used after sterilizing Rinse 4 times；（2）N+The old kind of ion beam mutation agate card：Embryo portion after agate card baked seed is put into sample towards ion beam mutation direction Product disk, start shooting preheating 2h before ion implanting, under vacuo with energy as 35KeV, line size 10mA, each burst length be 5s, target chamber vacuum are 0.9 × 10-4~1.05 × 10-3Pa carries out N+Ion beam mutation（In processing procedure, vacuum degree in vacuum chamber Faint change occurs）, N+Ion beam mutation dosage is 1.1 × 1037ion/cm2, then nursery, survey germination percentage；
4th group only with ultrasonication, specially：（1）Old kind of sterilizing of agate card：After the pending old kind cleaning of agate card It is put into culture dish, 0.6% liquor natrii hypochloritis is added in culture dish, is sterilized 17.5min, and ultrapure water 4 is used after sterilizing It is secondary；（2）The old kind of low temperature drying of agate card is preserved, nursery after ultrasonication：Agate card it is old plant drying after be placed in temperature less than 5 DEG C, it is wet Degree is preserved under the low temperature drying environment less than 45%, with 20% polyglycol solution seed soaking 20h before nursery, then with 60kHz ultrasounds Ripple processes 10min, then takes out, seeding and seedling raising after presoaking and germinating 2d, surveys germination percentage；
5th group only with chemicals treatment and N+Ion beam mutation treatment, specially：（1）Old kind of sterilizing of agate card：Will be pending Agate card it is old plant cleaning after be put into culture dish, add 0.6% liquor natrii hypochloritis in culture dish, sterilize 17.5min, sterilizing Use ultrapure water 4 times afterwards；（2）Old kind of activating agent immersion treatment of agate card：The old kind of agate card is soaked first in lower concentrations of active agent（It is low Concentration active agent component and proportioning are：ZnSO422.5g/L, CuSO4·5H2O 11g/L, MnCl27g/L）Middle immersion 3h, so Seed is taken out afterwards, is dried, the activating agent of high concentration is sprayed with watering can（Concentration of component is lower concentrations of active in high concentration activating agent 13.5 times of agent）And stirring agate card type makes seed uniformly soak, and dries again, repeatedly by baked seed after 3 times；（3）N+From Beamlet injects the old kind of agate card：Agate card seed embryo portion is put into sample disc towards ion beam mutation direction, is started shooting before ion implanting pre- Hot 2h, under vacuo with energy as 35KeV, line size 10mA, each burst length as 5s, target chamber vacuum be 0.9 × 10-4 ~1.05 × 10-3Pa carries out N+Ion beam mutation（In processing procedure, vacuum degree in vacuum chamber occurs faint change）, N+Ion beam Implantation dosage is 1.1 × 1037ion/cm2, then seeding and seedling raising, surveys germination percentage；
6th group only with chemicals treatment and ultrasonication, specially：（1）Old kind of sterilizing of agate card：By pending agate card It is put into culture dish after old kind cleaning, 0.6% liquor natrii hypochloritis is added in culture dish, sterilize 17.5min, uses super after sterilizing Pure water rinsing 4 times；（2）Old kind of activating agent immersion treatment of agate card：The old kind of agate card is soaked first in lower concentrations of active agent（Low concentration is lived Property agent component and proportioning be：ZnSO422.5g/L, CuSO4·5H2O 11g/L, MnCl27g/L）Middle immersion 3h, then will plant Son is taken out, and dries, and the activating agent of high concentration is sprayed with watering can（Concentration of component is lower concentrations of active agent in high concentration activating agent 13.5 times）And stirring agate card type makes seed uniformly soak, and dries again, repeatedly by baked seed after 3 times；（3）The old kind of agate card Low temperature drying is preserved, nursery after ultrasonication：The old kind of agate card is placed in temperature less than 5 DEG C, low temperature drying of the humidity less than 45% Preserved under environment, with 20% polyglycol solution seed soaking 20h before nursery, then with 60kHz ultrasonications 10min, Ran Houqu Go out, seeding and seedling raising after presoaking and germinating 2d, survey germination percentage；
7th group only with N+Ion beam mutation treatment and ultrasonication, specially：（1）Old kind of sterilizing of agate card：To wait to locate It is put into culture dish after the old kind cleaning of agate card of reason, 0.6% liquor natrii hypochloritis is added in culture dish, sterilizes 17.5min, goes out Ultrapure water is used after bacterium 4 times；（2）N+The old kind of ion beam mutation agate card：Embryo portion after agate card baked seed is noted towards ion beam Enter direction and be put into sample disc, before ion implanting start shooting preheating 2h, under vacuo with energy as 35KeV, line size 10mA, every time Burst length be 5s, target chamber vacuum be 0.9 × 10-4~1.05 × 10-3Pa carries out N+Ion beam mutation（In processing procedure, very Empty room vacuum occurs faint change）, N+Ion beam mutation dosage is 1.1 × 1037ion/cm2；（3）Old kind of low temperature of agate card is done Dry preservation, nursery after ultrasonication：The old kind of agate card after ion beam mutation is placed in temperature and is less than 45% less than 5 DEG C, humidity Preserved under low temperature drying environment, with 20% polyglycol solution seed soaking 20h before nursery, then with 60kHz ultrasonication 10min, Then take out, seeding and seedling raising after presoaking and germinating 2d, survey germination percentage；
8th group is left intact, and for blank, surveys the germination percentage of the old kind of agate card.The old kind of agate card under Different treatments Germination percentage test result it is as shown in table 1 below.
By the Different treatments of table 1 on old kind of result of the test of germination percentage influence of agate card it can be found that chemicals treatment, N+From Beamlet injects and ultrasonication can be to the germination percentage of agate card old kind generation positive result, but single treatment pattern（2nd, 3rd, 4 groups）Influence to bud rate is very limited（Gain 0.9%, 8.0%, 4.8% respectively）.Three kinds of modes process the old kind of agate card simultaneously（1st Group）, the germination percentage of the old kind of agate card is obviously improved, reached 58.6%, germination percentage gain 32.2%, and three kinds for the treatment of The enhancement effect sum for individually applying is only 13.7%, illustrates that the common applying of three kinds of processing modes generates synergy.
Analytical table 1 is it is also found that removal chemicals treatment or N+The dual factors test of ion beam mutation（6th, 7 groups）, knot Fruit agate card germination percentage is significantly reduced, and removes the processing mode of ultrasonication（5th group）, the germination percentage of the old kind of agate card still reaches To 51.4%, illustrate that synergy is mainly chemicals treatment and N+What ion beam mutation interaction was produced.
2nd, chemicals treatment and N+The analysis of ion beam mutation interaction mechanism
Ion beam mutation produces the differences such as biological effect and radiation, and it is Trinitarian that ion beam plays a role, including energy is heavy Product, quality deposition and charge-exchange.After agate card type is subject to ion beam mutation, its Physiology and biochemistry produces diversified change, So research ion beam and medicament interaction mechanism are extremely difficult.Usual condition, ion beam mutation can be sprouted to vegetable seeds Hair have depression effect, but under low dosage situation, ion beam mutation can improve the germination percentage of seed, this be due to low energy from Beamlet injection can be to hole be caused, so as to improve the result of the permeability of seed on seed cell parietal cell film.
Sputtering effect of the ion beam to organism can destroy cell membrane and membrane structure, be that allogenic material is carried into cell For good microchannel；The elecrtonegativity of cell surface can be reduced along with the quality deposition of ion implanting, acceptor is reduced external The electrostatic repulsion of source material；Low energy ion beam causes that medicament is sputtered into cell to the direct effect and indirectly-acting of organism In, the medicament for being deposited on seed cell surface is attacked by ion beam, so as to sputter into cell.Chemicals treatment is noted with ion beam Enter interaction Synergistic Mechanisms has difference again, and we are had found in studying, and medicament effect can be reduced using other medicaments or other proportionings, Increasing some other metal ion or even can produce depression effect, generating in illustrating element in medicament in the old kind of agate card Act on.
In the old kind of nursery of agate card, it has been found that ZnSO4 、CuSO4And MnCl2Combination and the cooperative effect of ion beam mutation Most preferably, wherein, zinc is relevant with the conversion of carbohydrate, can also promote the synthesis of protein, and copper is to activation of amino acid and albumen Matter synthesis has facilitation, and manganese can promote amino acid synthetic peptide, be conducive to protein to synthesize, and can also promote hydrolase polypeptide to generate ammonia Base acid, and transport the tissue and organ of new life to, and as ZnSO4 、CuSO4And MnCl2How to be played a role after sputtering seed , its specific mechanism of action need further research.
The present invention passes through chemicals treatment, N+The cooperative effect of ion beam mutation and ultrasonication, substantially increases agate card The viability and emergence rate of old kind, protection and utilization, agate card production and the raising of economic benefit to agate card germ plasm resource have It is significant.
Above example is only explanation technological thought of the invention, it is impossible to limit protection scope of the present invention with this, every According to technological thought proposed by the present invention, any change done on the basis of technical scheme each falls within the scope of the present invention Within；The technology that the present invention is not directed to can be realized by prior art.

Claims (4)

  1. It is 1. a kind of to improve old kind of method of survival rate of seedling of agate card, it is characterised in that：Described raising old kind of survival rate of seedling of agate card The step of method is：
    （1）Old kind of sterilizing of agate card：To be put into culture dish after the pending old kind cleaning of agate card, added in culture dish 0.6% liquor natrii hypochloritis, sterilized 15~20min, and ultrapure water is used 3~5 times after sterilizing；
    （2）Old kind of activating agent immersion treatment of agate card：The old kind of agate card is soaked into first 2~4h of immersion in lower concentrations of active agent, then Seed is taken out, is dried, sprayed the activating agent of high concentration with watering can and stirring agate card type makes seed uniformly soak, dried in the air again It is dry, repeatedly by baked seed after 2~3 times；
    （3）N+The old kind of ion beam mutation agate card：Agate card seed embryo portion is put into sample disc, ion note towards ion beam mutation direction Start preheating 2h before entering, under vacuo with energy as 35KeV, line size 10mA, each burst length as 5s, target chamber vacuum It is 6.0 × 10-4~1.3 × 10-3Pa carries out N+Ion beam mutation；
    （4）The old kind of low temperature drying of agate card is preserved, nursery after ultrasonication：By the old kind of low temperature drying of agate card after ion beam mutation Preserve, with 20% polyglycol solution seed soaking 20h before nursery, then with 55~65kHz ultrasonication 10min, then take out, Seeding and seedling raising after 2~3d of presoaking and germinating.
  2. It is 2. according to claim 1 to improve old kind of method of survival rate of seedling of agate card, it is characterised in that：The step（2） Middle lower concentrations of active agent component and proportioning are：ZnSO420~25g/L, CuSO4·5H2O 10~12g/L, MnCl26~8g/ L；Concentration of component is 12~15 times of lower concentrations of active agent in the high concentration activating agent.
  3. It is 3. according to claim 1 to improve old kind of method of survival rate of seedling of agate card, it is characterised in that：The step（3） Middle N+Ion beam mutation dosage is 0.9 × 1037ion/cm2~1.3 × 1037ion/cm2。
  4. It is 4. according to claim 1 to improve old kind of method of survival rate of seedling of agate card, it is characterised in that：The step（4） Middle low temperature drying is preserved and refers to temperature less than 5 DEG C, and humidity is less than 45%.
CN201710118688.7A 2017-03-02 2017-03-02 A kind of raising old kind of method of survival rate of seedling of agate card Pending CN106888620A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110786209A (en) * 2019-11-28 2020-02-14 河南中医药大学 Cultivation method for improving survival rate of selfheal seedlings and yield of selfheal

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Application publication date: 20170627