CN106880765B - Powder for promoting wound healing - Google Patents
Powder for promoting wound healing Download PDFInfo
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- CN106880765B CN106880765B CN201710148737.1A CN201710148737A CN106880765B CN 106880765 B CN106880765 B CN 106880765B CN 201710148737 A CN201710148737 A CN 201710148737A CN 106880765 B CN106880765 B CN 106880765B
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- powder
- hyaluronic acid
- wound healing
- dendrobium officinale
- wound
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
- A61K31/728—Hyaluronic acid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/38—Silver; Compounds thereof
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Dermatology (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Inorganic Chemistry (AREA)
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- Wood Science & Technology (AREA)
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to the field of medicines, and particularly relates to a method for constructing a trauma animal model and promoting wound healing. The method for promoting wound healing takes phenytoin silver, dendrobium officinale and hyaluronic acid as active ingredients, is externally applied to the wound, has the effects of stopping bleeding, easing pain, relieving itching, inhibiting bacterial infection of the wound, promoting wound healing and reducing scars, can be widely used for treating various traumas such as incised wound, traumatic injury, burn and scald, frostbite and the like, can obviously shorten the healing time of various traumas, relieves pain and pruritus of the wound and reduces the area of scars.
Description
Technical Field
The invention belongs to the field of medicines, and particularly relates to a method for constructing a trauma animal model and promoting wound healing.
Background
The skin is the protective tissue of the body surface of the animal, is composed of epidermis, dermis and subcutaneous tissue, is an important barrier of the animal body, can prevent various tissues and organs in the animal body from being invaded and damaged by physical, chemical or pathogenic bacteria, and maintains the relative stability of the environment in the animal body. Trauma is one type of skin injury, and when the skin is injured, regardless of the size of the wound, bleeding, bacterial infection, suppuration, ulceration, even systemic infection, tetanus and the like can be caused if the skin is not effectively treated, so that the life of an animal can be seriously threatened. In addition, severe pain is often caused when trauma is serious, itching is also accompanied in wound infection, ulceration and healing processes, the daily life of a patient is seriously influenced, when large-area epidermis or dermis is damaged due to trauma, the skin cannot be regenerated, scars are easily generated even if the wound is healed, and troubles are brought to the life of the patient after the wound is healed. Therefore, the trauma should be treated timely and effectively to avoid more damage to the animal.
Although the existing medicines for trauma are of various types, including Yunnan white medicines, band-aid and the like, the medicines are relatively single in function, are usually only one of the functions of easing pain, promoting blood circulation to remove blood stasis, inhibiting infection and the like, and cannot simultaneously improve the life quality of a patient, shorten the healing time of a wound and reduce the scar area after the wound is healed. Accordingly, the present invention is directed to providing a pharmaceutical composition effective in promoting wound healing.
Disclosure of Invention
An object of the present invention is to provide a method for promoting wound healing, which has hemostatic, analgesic, antipruritic, wound bacterial infection inhibiting, wound healing promoting and scar reducing effects by topically coating a traumatic wound with phenytoin silver, dendrobium officinale and hyaluronic acid as active ingredients.
The invention also aims to provide a pharmaceutical composition for promoting wound healing, which comprises the active ingredients of phenytoin silver, dendrobium officinale and hyaluronic acid.
The wound is trauma, preferably, the trauma is selected from knife wound, traumatic injury, burn and scald, frostbite and the like.
The weight ratio of the phenytoin silver to the dendrobium officinale to the hyaluronic acid is 1: 10-20: 10-25, and preferably the weight ratio of the phenytoin silver to the dendrobium officinale to the hyaluronic acid is 1: 15: 20.
The pharmaceutical composition for promoting wound healing is a topical preparation, preferably, the topical preparation can be a spray, an ointment, a patch, a powder, granules or a lotion and the like, and more preferably, the pharmaceutical composition for promoting wound healing further comprises a film-forming agent and/or a film-forming auxiliary agent.
Still another object of the present invention is to provide the use of phenytoin silver, dendrobium officinale and hyaluronic acid for preparing a pharmaceutical composition for promoting wound healing.
The wound is trauma, preferably, the trauma is selected from knife wound, traumatic injury, burn and scald, frostbite and the like.
The weight ratio of the phenytoin silver to the dendrobium officinale to the hyaluronic acid is 1: 10-20: 10-25, and preferably the weight ratio of the phenytoin silver to the dendrobium officinale to the hyaluronic acid is 1: 15: 20.
Phenytoin is an antiepileptic and antiarrhythmic drug and has a certain analgesic effect, silver ions are one of common antibacterial drugs in the field, and CN 104016923A discloses a phenytoin derivative, wherein sodium phenytoin and a silver ammonia solution are subjected to chemical reaction to obtain a silver salt of a compound 5, 5-diphenyl-2, 4 imidazolidinedione, and the silver salt is named as phenytoin silver.
The Dendrobium officinale is a plant of the genus Dendrobium of the family Orchidaceae, and can nourish yin, nourish body fluid, tonify spleen and stomach, and nourish skin, and modern pharmacological studies also show that the Dendrobium officinale also has the efficacy of hemostasis and can be used for trauma hemostasis.
Hyaluronic acid, also known as uronic acid and hyaluronic acid, is widely present in various parts of the human body, wherein the skin contains a large amount of hyaluronic acid, and has the effects of heat preservation, skin tendering, wrinkle removal and aging prevention, and meanwhile, hyaluronic acid is also an effective transdermal absorption enhancer which can effectively promote the transdermal absorption of nutrient components, so that the hyaluronic acid is widely applied to various cosmetics.
The invention has the beneficial effects that:
the pharmaceutical composition for promoting wound healing has simple composition and low cost, is suitable for industrial production, has multiple effects, is convenient to apply, has no stimulation, no toxic or side effect and high patient compliance, can obviously inhibit bleeding of traumatic wounds, inhibit pain caused by the trauma, inhibit wound pruritus in the healing process of the traumatic wounds and caused by infection, prevent infection of the traumatic wounds, promote healing of the traumatic wounds, shorten the healing time of the traumatic wounds, reduce the generation of traumatic scars and improve the life quality of the patients in the trauma and prognosis.
Detailed Description
The invention may be further understood by reference to the following examples, which illustrate some methods of making or using. However, it is to be understood that these examples do not limit the present invention. Variations of the invention, now known or further developed, are considered to fall within the scope of the invention as described herein and claimed below.
Example 1: a powder for promoting wound healing.
The powder agent for promoting wound healing consists of the following components in parts by weight:
10g of phenytoin silver, 100g of dendrobium officinale, 100g of hyaluronic acid and 790g of powder auxiliary materials.
The preparation method comprises the following steps:
(1) respectively crushing the dendrobium officinale, the hyaluronic acid and the powder auxiliary materials, and then sieving the crushed materials with a 100-mesh sieve, wherein the dendrobium officinale, the hyaluronic acid and the powder auxiliary materials are respectively equally divided into 4 parts for later use;
(2) taking phenytoin silver, respectively taking 1 part of the equant dendrobium officinale, hyaluronic acid and powder auxiliary material powder, adding the mixture into a three-dimensional mixer, mixing for 30 minutes, adding 1 part of the equant dendrobium officinale, hyaluronic acid and powder auxiliary material powder until all the components are added, and mixing for 30 minutes to obtain the powder for promoting wound healing.
Example 2: a spray for promoting wound healing.
The spray for promoting wound healing consists of the following components in parts by weight:
10g of phenytoin silver, 150g of dendrobium officinale, 200g of hyaluronic acid and 640g of auxiliary spray.
The spray for promoting wound healing of the invention is prepared according to the conventional preparation method of the spray in the field.
Example 3: a lotion for promoting wound healing is provided.
The lotion for promoting wound healing comprises the following components in parts by weight:
10g of phenytoin silver, 200g of dendrobium officinale, 250g of hyaluronic acid and 1540g of lotion auxiliary materials.
The lotion for promoting wound healing of the invention is prepared according to the conventional preparation method of the lotion in the field.
Comparative examples 1 to 3: powder for promoting wound healing
Powders for promoting wound healing of comparative examples 1 to 3 were prepared according to the method of example 1 except that comparative example 1 contains phenytoin silver (20g) and dendrobium officinale (190g) as active ingredients, comparative example 2 contains phenytoin silver (20g) and hyaluronic acid (340g) as active ingredients, and comparative example 3 contains only powder excipients, and the amounts and preparation methods of the ingredients were the same as those of example 1.
Example of effects: effect of the pharmaceutical composition of the present invention on the healing time of traumatic wound in rats
1. The experimental method comprises the following steps:
1.1 Experimental animals:
10 male SD rats weighing 180-.
1.2 establishment of rat trauma model
10 SD rats are marked as No. 1-10 in sequence, after anaesthesia of the rats, the backs are dehaired, the backs are disinfected 3 times with 75% alcohol, each time at 1 minute intervals, 4 blocks of 1.5 ＊ 1.5.5 cm of area are drawn out symmetrically on the backs of the rats using purple liquid medicine, recorded as "serial number-1-4", such as 1-1, 1-2, 1-3, 1-4 … … 10-1, 10-2, 10-3, 10-4, wherein areas 1-4 are recorded as example 1 group and comparative examples 1-3 group, respectively, and the powders of example 1 and comparative examples 1-3 are applied, 2 g/time per area, the skin of the areas 1-4 is cut off to the muscle layer using sterile surgical scissors and the fascia layer is prevented from being damaged, the corresponding powders are applied immediately after the completion of each area surgery, covered with gauze, fixed, and the application time is recorded.
The drugs were changed every other day, and the healing time of each area, i.e. the wound surface was filled with new granulation tissue and completely epithelialized, was recorded and the average healing time of each area was calculated.
2. The statistical method comprises the following steps:
SPSS17.0 statistical software is adopted, data are expressed by x +/-s (s is standard deviation), single factor analysis is adopted, Student's-ttest is adopted for comparison between two groups, variance analysis is adopted for comparison between the mean values of a plurality of samples, and P is less than 0.05 and is used as a significance test standard.
3. The experimental results are as follows:
the average healing time of example 1 and comparative examples 1 to 3 is shown in Table 1.
Table 1 mean healing time (x ± s, n ═ 10)
Note that ＊ P compared with control 3, P < 0.05, ＊＊ P compared with control 3, P < 0.01.
Table 1 shows that, comparative examples 1-2 in which phenytoin silver and dendrobium officinale, and phenytoin silver and hyaluronic acid are active ingredients have shown significantly shortened healing time compared to comparative example 3 in which no active ingredient is included, while the inventive powder for promoting wound healing of example 1 has significantly shorter average healing time, shows that after the phenytoin silver is compounded with dendrobium officinale, and hyaluronic acid, the three act synergistically, has an efficient effect of promoting wound healing, and can be widely applied to the treatment of various traumas.
Claims (1)
1. The powder for promoting wound healing is characterized by comprising the following components in parts by weight: 10g of phenytoin silver, 100g of dendrobium officinale, 100g of hyaluronic acid and 790g of powder auxiliary materials, wherein the wound is a trauma, and the trauma is selected from knife wound, traumatic injury, burn and scald or frostbite, and the preparation method comprises the following steps:
(1) respectively crushing the dendrobium officinale, the hyaluronic acid and the powder auxiliary materials, and then sieving the crushed materials with a 100-mesh sieve, wherein the dendrobium officinale, the hyaluronic acid and the powder auxiliary materials are respectively equally divided into 4 parts for later use;
(2) taking phenytoin silver, respectively taking 1 part of the equant dendrobium officinale, hyaluronic acid and powder auxiliary material powder, adding the mixture into a three-dimensional mixer, mixing for 30 minutes, adding 1 part of the equant dendrobium officinale, hyaluronic acid and powder auxiliary material powder every time, mixing for 30 minutes until all the components are added, and obtaining the powder for promoting wound healing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710148737.1A CN106880765B (en) | 2017-03-13 | 2017-03-13 | Powder for promoting wound healing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710148737.1A CN106880765B (en) | 2017-03-13 | 2017-03-13 | Powder for promoting wound healing |
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| Publication Number | Publication Date |
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| CN106880765A CN106880765A (en) | 2017-06-23 |
| CN106880765B true CN106880765B (en) | 2020-05-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710148737.1A Active CN106880765B (en) | 2017-03-13 | 2017-03-13 | Powder for promoting wound healing |
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| Country | Link |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108635513A (en) * | 2018-08-08 | 2018-10-12 | 李琴 | A kind of drug that treating burn and scald, preparation method and application |
| CN113476668A (en) * | 2021-07-12 | 2021-10-08 | 中山大学附属第六医院 | Biodegradable anastomosis nail and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103127394A (en) * | 2013-03-05 | 2013-06-05 | 云南农业大学 | Dendrobium extract product application |
| CN103191461A (en) * | 2013-04-26 | 2013-07-10 | 华熙福瑞达生物医药有限公司 | Wound nursing dressing containing hyaluronic acid |
| CN104016923A (en) * | 2014-01-08 | 2014-09-03 | 南开大学 | Phenytoin derivative as well as preparation method and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103751555A (en) * | 2013-12-18 | 2014-04-30 | 广西健宝石斛有限责任公司 | Dendrobium officinale patch with hemostatic function |
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- 2017-03-13 CN CN201710148737.1A patent/CN106880765B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103127394A (en) * | 2013-03-05 | 2013-06-05 | 云南农业大学 | Dendrobium extract product application |
| CN103191461A (en) * | 2013-04-26 | 2013-07-10 | 华熙福瑞达生物医药有限公司 | Wound nursing dressing containing hyaluronic acid |
| CN104016923A (en) * | 2014-01-08 | 2014-09-03 | 南开大学 | Phenytoin derivative as well as preparation method and application thereof |
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| CN106880765A (en) | 2017-06-23 |
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