CN106880765A - A kind of structure of wound animal model and the method for promoting wound healing - Google Patents
A kind of structure of wound animal model and the method for promoting wound healing Download PDFInfo
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- CN106880765A CN106880765A CN201710148737.1A CN201710148737A CN106880765A CN 106880765 A CN106880765 A CN 106880765A CN 201710148737 A CN201710148737 A CN 201710148737A CN 106880765 A CN106880765 A CN 106880765A
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- wound
- wound healing
- pharmaceutical composition
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- hyaluronic acid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
- A61K31/728—Hyaluronic acid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/38—Silver; Compounds thereof
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- Veterinary Medicine (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Animal Behavior & Ethology (AREA)
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Abstract
The invention belongs to drug field, and in particular to a kind of structure of wound animal model and the method for promoting wound healing.The method for promoting wound healing of the invention is using phenytoinum naticum silver, dendrobium candidum and hyaluronic acid as active component, external application coats trauma wounds, with hemostasis, analgesia, effect that is antipruritic, suppressing bacterial wound infection, promotion wound healing and reduction scar, can be widely used for the treatment of all kinds of wounds such as knife wound, traumatic injury, burn and scald, frostbite, the healing time of all kinds of trauma wounds can significantly be shortened, mitigate wound pain and itch, reduce the area of scar.
Description
Technical field
The invention belongs to drug field, and in particular to a kind of structure of wound animal model and the side of promotion wound healing
Method.
Background technology
Skin is the protective tissue of animal body surface, is made up of epidermis, corium and hypodermis, is the important screen of animal body
Barrier, can avoid each tissue and internal organs in animal body from being subject to the invasion and attack and destruction of physics, chemistry or pathogen, maintain animal body inner ring
Border relatively stablize.Wound is one of type of skin injury, when damaging occurs in skin, no matter the size of wound, if not
Effectively to process be possible to and cause bleeding, bacterium infection, suppurate, fester, or even general infection, lockjaw etc. can be triggered, sternly
The life of the jeopardized animal of weight.Also, often cause violent pain when wound is serious, wound infection, fester and heal
During be accompanied by itch etc., the daily life of patient has been had a strong impact on, when wound causes large surface of skin or corium impaired
When, skin can be caused to regenerate, now cicatrizing a wound also easilys lead to the generation of scar, is after patient wound heals
Life bring puzzlement.Therefore, should timely and effectively be processed for wound, it is to avoid bigger damage is produced to animal body
Evil.
Although the current existing medicine species for being directed to wound is more, including Yunnan Baiyao, adhesive bandage etc., its function
It is relatively single, it is only often one of function such as analgesia, promoting blood circulation and removing blood stasis, suppression infection, can not simultaneously improve the life matter of patient
Scar area after amount, the healing time for shortening wound, reduction wound healing.Therefore, this invention address that providing a kind of effective
Promote the pharmaceutical composition of wound healing.
The content of the invention
It is an object of the present invention to provide a kind of method for promoting wound healing, methods described is with phenytoinum naticum silver, iron sheet
The stem of noble dendrobium and hyaluronic acid are used as active component, external application coating trauma wounds, with hemostasis, analgesia, antipruritic, suppression bacterial wound sense
Dye, the effect for promoting wound healing and reduction scar.
It is a further object to provide a kind of pharmaceutical composition of promotion wound healing, its active component includes benzene
Appropriate English silver, dendrobium candidum and hyaluronic acid.
The wound is wound, it is preferable that the wound is selected from knife wound, traumatic injury, burn and scald or frostbite etc..
The weight ratio of the phenytoinum naticum silver, dendrobium candidum and hyaluronic acid is 1: 10-20: 10-25, it is preferable that the benzene
The weight ratio of appropriate English silver, dendrobium candidum and hyaluronic acid is 1: 15: 20.
The pharmaceutical composition of promotion wound healing of the present invention is topical formulations, it is preferable that the topical formulations can
Think spray, paste, patch, pulvis, powder, granule or lotion etc., the medicine of the preferred promotion wound healing
Film forming agent and/or coalescents are also included in composition.
Prepared it is also another object of the present invention to provide phenytoinum naticum silver, dendrobium candidum and hyaluronic acid and promote wound healing
Purposes in pharmaceutical composition.
The wound is wound, it is preferable that the wound is selected from knife wound, traumatic injury, burn and scald or frostbite etc..
The weight ratio of the phenytoinum naticum silver, dendrobium candidum and hyaluronic acid is 1: 10-20: 10-25, it is preferable that the benzene
The weight ratio of appropriate English silver, dendrobium candidum and hyaluronic acid is 1: 15: 20.
Phenytoinum naticum is anti-epileptic, antiarrhythmic drug, and with certain analgesic effect, and silver ion is that this area is common
One of antibacterial medicines, CN104016923 A disclose a kind of derivative of phenytoinum naticum, and it is by molten with silver-colored ammonia by dilantin sodium
Liquid carries out chemical reaction and obtains compound 5,5- diphenyl -2, the silver salt of 4 imidazolidimediones, and is named as phenytoinum naticum
Silver, the patent also discloses its phenytoinum naticum silver has antibacterial, sterilization, suppresses wound infection and promotes the effect of wound healing.
Dendrobium candidum is orchid family dendrobium plant, can nourish yin jin, strengthen the spleen and stomach, nourish skin, modern pharmacology grinds
Study carefully also show dendrobium candidum also have functions that hemostasis, can be used for trauma hemostasis.
Hyaluronic acid is widely present in partes corporis humani position, i.e. containing substantial amounts of wherein in skin also known as uronic acid, sodium hyaluronate
Hyaluronic acid, it has insulation, tender skin, goes wrinkle and anti-aging effect, while hyaluronic acid or effective Transdermal absorption promote
Enter agent, its Transdermal absorption that can effectively facilitate nutritional ingredient, therefore, it is widely used in all kinds of cosmetics.
Beneficial effects of the present invention：
The pharmaceutical composition composition for promoting wound healing of the invention is simple, with low cost, is adapted to industrialized production, and its
It is non-stimulated using convenient with multi-efficiency, have no toxic side effect, patient compliance is high, can significantly inhibit going out for trauma wounds
Blood, suppresses pain caused by wound, in suppression trauma wounds agglutination and due to wound itch caused by infection, prevents outer
The infection of wound, promotes the healing of trauma wounds, shortens the healing time of trauma wounds, and reduces the generation of wound scar,
Improve patient wound in and prognosis quality of life.
Specific embodiment
Also the present invention further can be understood by embodiment, wherein the embodiment illustrates that some are prepared or user
Method.It is to be appreciated, however, that these embodiments do not limit the present invention.Currently known or further exploitation change of the invention
Change is considered within the scope of the invention described herein and claimed below.
Embodiment 1：A kind of pulvis of promotion wound healing.
The pulvis agent for promoting wound healing is by following weight proportion into being grouped into：
Phenytoinum naticum silver 10g, dendrobium candidum 100g, hyaluronic acid 100g, pulvis auxiliary material 790g.
Its preparation method includes：
(1) after dendrobium candidum, hyaluronic acid and pulvis auxiliary material are crushed respectively cross 100 mesh sieves, dendrobium candidum, hyaluronic acid with
Pulvis auxiliary material is divided into 4 parts respectively, standby；
(2) phenytoinum naticum silver is taken, and respectively takes the dendrobium candidum after 1 part of decile, hyaluronic acid and pulvis auxiliary material powder and add three
Mix in dimension mixer, often mix the dendrobium candidum after adding 1 part of decile in 30 minutes, hyaluronic acid and pulvis auxiliary material powder, directly
To after all adding, the pulvis for obtaining final product promotion wound healing of the present invention for 30 minutes is remixed.
Embodiment 2：A kind of spray of promotion wound healing.
The spray for promoting wound healing is by following weight proportion into being grouped into：
Phenytoinum naticum silver 10g, dendrobium candidum 150g, hyaluronic acid 200g, spray auxiliary material 640g.
Customary preparation methods according to this area spray are prepared and obtain final product the spray that the present invention promotes wound healing.
Embodiment 3：A kind of lotion of promotion wound healing.
The lotion for promoting wound healing is by following weight proportion into being grouped into：
Phenytoinum naticum silver 10g, dendrobium candidum 200g, hyaluronic acid 250g, lotion auxiliary material 1540g.
Customary preparation methods according to this area lotion are prepared and obtain final product the lotion that the present invention promotes wound healing.
Comparative example 1-3：A kind of pulvis of promotion wound healing
Method according to embodiment 1 prepares the pulvis of the promotion wound healing of comparative example 1-3, and its difference is comparative example 1
It is that active component, comparative example 2 include phenytoinum naticum silver (20g) and hyalomitome comprising phenytoinum naticum silver (20g) and dendrobium candidum (190g)
Sour (340g) is that active component, comparative example 3 only include pulvis auxiliary material, consumption and preparation method and the embodiment 1 of each composition
It is identical.
Effect example：Influence of the pharmaceutical composition of the present invention to rat trauma wounds healing time
1st, experimental technique：
1.1 experimental animals：
10 male SD rats, body weight 180-220g.
The foundation of 1.2 rat surgical wound models
10 SD rats are sequentially labeled as No. 1-10, and after rat anesthesia, back unhairing uses 75% alcohol disinfecting back 3
Secondary, every minor tick 1 minute marks 4 pieces of the region of 1.5*1.5cm using gentian violet in rat back symmetry, records respectively
It is " sequence number -1-4 ", such as 1-1,1-2,1-3,1-4 ... 10-1,10-2,10-3,10-4 that wherein region 1-4 is recorded as reality respectively
1 group of example and comparative example 1-3 groups are applied, and applies embodiment 1, the pulvis of comparative example 1-3 respectively, be 2g/ times per Zoned application amount.Make
The skin of the region 1-4 is wiped out to muscle layer with sterilizing surgical scissors, and prevents from damaging fascia layer, each region has been performed the operation
Apply corresponding pulvis immediately after, covered using gauze, fixed, and the recording medicine time.
Every other day change medicine, and record the healing time in each region, calculate the average healing in each region, it is described
Healing refers to that the surface of a wound is filled up by new granulation tissue, and epithelialization completely.
2nd, statistical method：
Using SPSS17.0 statistical softwares, data with x ± s (s is standard deviation) represent, using single factor analysis, two groups it
Between compare and use Student ' s-ttest, compare between multiple sample averages and use variance analysis, P ＜ 0.05 are examined as conspicuousness
Test level.
3rd, experimental result：
Embodiment 1, the average healing of reference examples 1-3 are shown in Table 1.
The average healing of table 1 (x ± s, n=10)
Note：* P compares with reference examples 3, and P ＜ 0.05, * * P compare with reference examples 3, P ＜ 0.01.
The experimental result of table 1 shows that phenytoinum naticum silver is the contrast of active component with dendrobium candidum, phenytoinum naticum silver and hyaluronic acid
Example 1-2 has shown that the healing time of obvious shortening relative to the comparative example 3 not comprising active component, and this hair of embodiment 1
The pulvis of bright promotion wound healing has considerably shorter the average healing, show phenytoinum naticum silver with dendrobium candidum,
Hyaluronic acid compounding after, between three act synergistically, with the effect for being efficiently promoting wound healing, can extensively using with it is all kinds of
The treatment of wound.
Claims (10)
1. it is a kind of to promote wound healing pharmaceutical composition, it is characterised in that described pharmaceutical composition is with phenytoinum naticum silver, dendrobium candidum
It is active component with hyaluronic acid.
2. it is according to claim 1 to promote wound healing pharmaceutical composition, it is characterised in that the wound is wound.
3. it is according to claim 1 and 2 to promote wound healing pharmaceutical composition, it is characterised in that the wound is selected from knife
Wound, traumatic injury, burn and scald or frostbite etc..
4. it is according to claim 1 and 2 to promote wound healing pharmaceutical composition, it is characterised in that the phenytoinum naticum silver, iron
The weight ratio of the skin stem of noble dendrobium and hyaluronic acid is：1∶10-20∶10-25.
5. it is according to claim 4 to promote wound healing pharmaceutical composition, it is characterised in that the phenytoinum naticum silver, iron sheet
The weight ratio of the stem of noble dendrobium and hyaluronic acid is：1∶15∶20.
6. the promotion wound healing pharmaceutical composition according to claim 1-2 or 5, it is characterised in that the drug regimen
Thing is topical formulations.
7. it is according to claim 6 to promote wound healing pharmaceutical composition, it is characterised in that the topical formulations are selected from spray
Mist agent, paste, patch, pulvis, powder, granule or lotion.
8. it is according to claim 7 to promote wound healing pharmaceutical composition, it is characterised in that to be wrapped in described pharmaceutical composition
Containing film forming agent and/or coalescents.
9. phenytoinum naticum silver, dendrobium candidum and hyaluronic acid are preparing the purposes in promoting wound healing pharmaceutical composition.
10. purposes according to claim 9, it is characterised in that the wound is wound.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710148737.1A CN106880765B (en) | 2017-03-13 | 2017-03-13 | Powder for promoting wound healing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710148737.1A CN106880765B (en) | 2017-03-13 | 2017-03-13 | Powder for promoting wound healing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106880765A true CN106880765A (en) | 2017-06-23 |
| CN106880765B CN106880765B (en) | 2020-05-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710148737.1A Active CN106880765B (en) | 2017-03-13 | 2017-03-13 | Powder for promoting wound healing |
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| Country | Link |
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| CN (1) | CN106880765B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108635513A (en) * | 2018-08-08 | 2018-10-12 | 李琴 | A kind of drug that treating burn and scald, preparation method and application |
| CN113476668A (en) * | 2021-07-12 | 2021-10-08 | 中山大学附属第六医院 | Biodegradable anastomosis nail and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103127394A (en) * | 2013-03-05 | 2013-06-05 | 云南农业大学 | Dendrobium extract product application |
| CN103191461A (en) * | 2013-04-26 | 2013-07-10 | 华熙福瑞达生物医药有限公司 | Wound nursing dressing containing hyaluronic acid |
| CN103751555A (en) * | 2013-12-18 | 2014-04-30 | 广西健宝石斛有限责任公司 | Dendrobium officinale patch with hemostatic function |
| CN104016923A (en) * | 2014-01-08 | 2014-09-03 | 南开大学 | Phenytoin derivative as well as preparation method and application thereof |
-
2017
- 2017-03-13 CN CN201710148737.1A patent/CN106880765B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103127394A (en) * | 2013-03-05 | 2013-06-05 | 云南农业大学 | Dendrobium extract product application |
| CN103191461A (en) * | 2013-04-26 | 2013-07-10 | 华熙福瑞达生物医药有限公司 | Wound nursing dressing containing hyaluronic acid |
| CN103751555A (en) * | 2013-12-18 | 2014-04-30 | 广西健宝石斛有限责任公司 | Dendrobium officinale patch with hemostatic function |
| CN104016923A (en) * | 2014-01-08 | 2014-09-03 | 南开大学 | Phenytoin derivative as well as preparation method and application thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108635513A (en) * | 2018-08-08 | 2018-10-12 | 李琴 | A kind of drug that treating burn and scald, preparation method and application |
| CN113476668A (en) * | 2021-07-12 | 2021-10-08 | 中山大学附属第六医院 | Biodegradable anastomosis nail and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN106880765B (en) | 2020-05-12 |
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