CN106858229A - A kind of mango fermented beverage and preparation method thereof - Google Patents

A kind of mango fermented beverage and preparation method thereof Download PDF

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Publication number
CN106858229A
CN106858229A CN201510928920.4A CN201510928920A CN106858229A CN 106858229 A CN106858229 A CN 106858229A CN 201510928920 A CN201510928920 A CN 201510928920A CN 106858229 A CN106858229 A CN 106858229A
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mango
lactobacillus
fermented beverage
ratio
culture
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Inventor
杨明兵
周云喜
唐美艳
梁冰
韩慧敏
杨胜梅
张兰艳
刘潇
朱艳
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HUNAN SIQI BIOPHARMACEUTICAL Co Ltd
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HUNAN SIQI BIOPHARMACEUTICAL Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/125Casei
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum

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  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Preparation Of Fruits And Vegetables (AREA)

Abstract

The invention belongs to field of food, more particularly to a kind of mango fermented beverage and preparation method thereof.Described mango fermented beverage, by blanching, mashing, fermentation, seasoning, sterilizing and obtain.Mango fermented beverage provided by the present invention, nutritious, eating mouth feel is good, beneficial to nutrition intake and healthy.

Description

A kind of mango fermented beverage and preparation method thereof
Technical field
The invention belongs to food technology field, and in particular to a kind of mango fermented beverage.
Background technology:
Mango Fruit contains sugar, protein, crude fibre, and the precursor carrotene composition of the vitamin A contained by mango is especially high, It is rare in all fruit.Secondly Vitamin C content is not also low.Mineral matter, protein, fat, carbohydrate etc., are also it Main nutrient composition.One of famous tropical fruit (tree) of mango taste, pulp is fine and smooth, and unique flavor is very popular.But mango Long-term preservation is difficult to, it is more puckery to be made fruit drink mouthfeel, need to add the additives such as pigment, essence and preservative, unhealthful, Limit application of the mango in fruit drink field.
The content of the invention:
Present invention aim at a kind of mango fermented beverage is provided, nutritious, eating mouth feel is good, beneficial to nutrition intake and body Health.
To achieve the above object, the technical solution adopted by the present invention is as follows:
A kind of mango fermented beverage, fermented bacterium used is Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, secondary cheese breast The mixed bacteria of bacillus composition, the ratio of the mixed bacteria is 8-12:1-4:7-10:1.
A kind of mango fermented beverage, is made up of following steps:
(1) mango is cleaned, dried, and in input boiling water, blanching 5-10 seconds, is pulled out and is immediately placed in cooling in cold water, peeling;
(2) by mango mashing, the homogeneous after peeling;
(3) feed liquid after homogeneous is placed in fermentation tank, glucose, sucrose is separately added into by the 8%-12% of material liquid volume, mixed Close uniform, sterilizing;
(4) Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, lactobacillus paracasei are pressed into 8-12:1-4:7-10:1 ratio It is well mixed, in the feed liquid after being sterilized by the 1%-5% additions of material liquid volume, it is sufficiently stirred for, ferment, obtain mango juice;
(5) mango juice, purified water, sucrose are pressed 10:16:The ratio mixing of 1-5, by the 0.03%-0.05% of material liquid volume Vitamin C is added, is well mixed, homogeneous, it is sterilized, it is filling to obtain final product.
Above-mentioned mango fermented beverage, in the step (2), the mango after peeling presses solid-liquid ratio 1:The ratio of 0.5-1 adds purifying Water, stirs, and is beaten 10-30min.
Above-mentioned mango fermented beverage, in the step (2), homogenization cycles are 1-3 times, homogenization pressure 20-50Mpa.
Above-mentioned mango fermented beverage, in the step (2), homogenization cycles are 2 times, and first time homogenization pressure is 20-25Mpa, Second homogenization pressure is 35-40Mpa.
Above-mentioned mango fermented beverage, above-mentioned Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, 4 kinds of bacterium of lactobacillus paracasei Plant and used after following steps acclimation and screening:
(1) resistance to sugar high:Addition glucose is 40%, 50%, 60% using the ratio of glucose in MRS culture mediums, After being inoculated with above-mentioned 4 kinds of strains respectively, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, apply It is distributed on MRS culture medium flat plates, after cultivating 24h in 37 ± 1 DEG C, takes the dilution level of bacterium colony number 30-300CFU/ wares, chooses Single bacterium colony MRS cultures are taken based on 37 ± 1 DEG C of culture 24h, conservation;
(2) resistance to peracid:MRS culture mediums are adjusted into pH to 2.0,3.0,4.0 with watery hydrochloric acid, after being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat on MRS culture medium flat plates, in After 37 ± 1 DEG C of culture 24h, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking single bacterium colony is cultivated with MRS and is based on 37 ± 1 DEG C of culture 24h, conservation.
Above-mentioned mango fermented beverage, in the step (4), fermentation temperature is 34-38 DEG C, and fermentation time is 18-48h, extremely Stop fermentation during pH=3.5-3.8.
Above-mentioned mango fermented beverage, in the step (5), homogenization cycles are 1-3 times, homogenization pressure 20-40Mpa.
Above-mentioned mango fermented beverage, in the step (5), homogenization cycles are 2 times, and first time homogenization pressure is 20-25Mpa, Second homogenization pressure is 35-40Mpa.
Above-mentioned mango fermented beverage, in the step (5), sterilization mode is ultra high temperature short time sterilization, is added at 125-150 DEG C Hot 3-6s.
Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, secondary cheese breast in mango fermented beverage fermented bacterium of the present invention The ratio of bacillus is preferably 12:3:8:1.
Compared with prior art, the invention has the advantages that:
(1) the mango fermented beverage safety and Health obtained by the present invention, is sufficiently reserved nutritional ingredient, is of high nutritive value;
(2) present invention gained mango fermented beverage preparation method is simple, suitable industrialized production;
(3) present invention gained mango juice fermented beverage, by fermentation after, with the addition of the peculiar flavour of lactobacillus-fermented, it is and normal Rule mango juice is compared, and its mouthfeel is more preferably.
Gained mango fermented beverage of the invention, mouthfeel is unique, nutritious, be easy to be received by consumers in general, with very big Market potential.
Specific embodiment
Embodiment 1
(1) mango is cleaned, dried, and in input boiling water, blanching 5 seconds is pulled out and is immediately placed in cooling in cold water, peeling;
(2) mango after peeling is pressed into solid-liquid ratio 1:0.5 ratio adds purified water, stirs, and is beaten 20min, homogeneous 3 It is secondary, first time homogenization pressure 20-25Mpa, second homogenization pressure 20-25Mpa, third time homogenization pressure 35-40Mpa;
(3) feed liquid after homogeneous is placed in fermentation tank, glucose, sucrose is separately added into by the 8% of material liquid volume, mixing is equal It is even, sterilizing;
(4) Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, lactobacillus paracasei are pressed 8:2:10:1 ratio mixing is equal It is even, in the feed liquid after being sterilized by 1% addition of material liquid volume, it is sufficiently stirred for, ferment 47h at 34-38 DEG C, is hair to pH=3..8 Ferment terminal, obtains mango juice;
(5) mango juice, purified water, sucrose are pressed 10:16:1 ratio mixing, adds the vitamin of material liquid volume 0.05% C, is well mixed, homogeneous 1 time under 30-35Mpa, and 3s sterilizations are heated at 125-130 DEG C, filling to obtain final product.
Embodiment 2
(1) mango is cleaned, dried, and in input boiling water, blanching 5 seconds is pulled out and is immediately placed in cooling in cold water, peeling;
(2) mango after peeling is pressed into solid-liquid ratio 1:0.8 ratio adds purified water, stirs, and is beaten 10min, homogeneous 2 It is secondary, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa;
(3) feed liquid after homogeneous is placed in fermentation tank, glucose, sucrose is separately added into by the 12% of material liquid volume, mixing is equal It is even, sterilizing;
(4) Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, 4 kinds of strains of lactobacillus paracasei are tamed through following steps Used after screening:
A. it is resistance to high sugared:Addition glucose is 40%, 50%, 60% using the ratio of glucose, respectively in MRS culture mediums After being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat On MRS culture medium flat plates, after cultivating 24h in 37 ± 1 DEG C, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking is single Bacterium colony MRS is cultivated based on 37 ± 1 DEG C of culture 24h, conservation;
B. resistance to peracid:MRS culture mediums are adjusted into pH to 2.0,3.0,4.0 with watery hydrochloric acid, after being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat on MRS culture medium flat plates, in After 37 ± 1 DEG C of culture 24h, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking single bacterium colony is cultivated with MRS and is based on 37 ± 1 DEG C of culture 24h, conservation.
Strain after 4 kinds of domestications of the above presses 12:4:7:1 ratio is well mixed, by the feed liquid after 2% addition sterilizing of material liquid volume In, it is sufficiently stirred for, ferment 25h at 34-38 DEG C, is fermentation termination to pH=3.5, obtains mango juice;
(5) mango juice, purified water, sucrose are pressed 12:16:5 ratio mixing, adds the vitamin of material liquid volume 0.03% C, is well mixed, homogeneous 2 times, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa, 145-150 6s is heated at DEG C sterilized, it is filling to obtain final product.
Embodiment 3
(1) mango is cleaned, dried, and in input boiling water, blanching 10 seconds is pulled out and is immediately placed in cooling in cold water, peeling;
(2) mango after peeling is pressed into solid-liquid ratio 1:1 ratio adds purified water, stirs, and is beaten 30min, homogeneous 2 It is secondary, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa;
(3) feed liquid after homogeneous is placed in fermentation tank, glucose, sucrose is separately added into by the 11% of material liquid volume, mixing is equal It is even, sterilizing;
(4) Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, 4 kinds of strains of lactobacillus paracasei are tamed through following steps Used after screening:
A. it is resistance to high sugared:Addition glucose is 40%, 50%, 60% using the ratio of glucose, respectively in MRS culture mediums After being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat On MRS culture medium flat plates, after cultivating 24h in 37 ± 1 DEG C, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking is single Bacterium colony MRS is cultivated based on 37 ± 1 DEG C of culture 24h, conservation;
B. resistance to peracid:MRS culture mediums are adjusted into pH to 2.0,3.0,4.0 with watery hydrochloric acid, after being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat on MRS culture medium flat plates, in After 37 ± 1 DEG C of culture 24h, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking single bacterium colony is cultivated with MRS and is based on 37 ± 1 DEG C of culture 24h, conservation.
Strain after 4 kinds of domestications of the above presses 10:3:8:1 ratio is well mixed, by the feed liquid after 5% addition sterilizing of material liquid volume In, it is sufficiently stirred for, ferment 29h at 34-38 DEG C, is fermentation termination to pH=3.6, obtains mango juice;
(5) mango juice, purified water, sucrose are pressed 10:16:3 ratio mixing, adds the vitamin of material liquid volume 0.05% C, is well mixed, homogeneous 2 times, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa, 130-140 4s is heated at DEG C sterilized, it is filling to obtain final product.
Embodiment 4
(1) mango is cleaned, dried, and in input boiling water, blanching 8 seconds is pulled out and is immediately placed in cooling in cold water, peeling;
(2) mango after peeling is pressed into solid-liquid ratio 1:0.8 ratio adds purified water, stirs, and is beaten 25min, homogeneous 2 It is secondary, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa;
(3) feed liquid after homogeneous is placed in fermentation tank, glucose, sucrose is separately added into by the 10% of material liquid volume, mixing is equal It is even, sterilizing;
(4) Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, 4 kinds of strains of lactobacillus paracasei are tamed through following steps Used after screening:
A. it is resistance to high sugared:Addition glucose is 40%, 50%, 60% using the ratio of glucose, respectively in MRS culture mediums After being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat On MRS culture medium flat plates, after cultivating 24h in 37 ± 1 DEG C, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking is single Bacterium colony MRS is cultivated based on 37 ± 1 DEG C of culture 24h, conservation;
B. resistance to peracid:MRS culture mediums are adjusted into pH to 2.0,3.0,4.0 with watery hydrochloric acid, after being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat on MRS culture medium flat plates, in After 37 ± 1 DEG C of culture 24h, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking single bacterium colony is cultivated with MRS and is based on 37 ± 1 DEG C of culture 24h, conservation.
Strain after 4 kinds of domestications of the above presses 10:1:10:1 ratio is well mixed, by the material after 3% addition sterilizing of material liquid volume In liquid, it is sufficiently stirred for, ferment 20h at 34-38 DEG C, is fermentation termination to pH=3.6, obtains mango juice;
(5) mango juice, purified water, sucrose are pressed 10:16:2 ratio mixing, adds the vitamin of material liquid volume 0.04% C, is well mixed, homogeneous 2 times, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa, 125-135 5s is heated at DEG C sterilized, it is filling to obtain final product.
Embodiment 5
(1) mango is cleaned, dried, and in input boiling water, blanching 6 seconds is pulled out and is immediately placed in cooling in cold water, peeling;
(2) mango after peeling is pressed into solid-liquid ratio 1:0.8 ratio adds purified water, stirs, and is beaten 25min, homogeneous 2 It is secondary, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa;
(3) feed liquid after homogeneous is placed in fermentation tank, glucose, sucrose is separately added into by the 10% of material liquid volume, mixing is equal It is even, sterilizing;
(4) Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, 4 kinds of strains of lactobacillus paracasei are tamed through following steps Used after screening:
A. it is resistance to high sugared:Addition glucose is 40%, 50%, 60% using the ratio of glucose, respectively in MRS culture mediums After being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat On MRS culture medium flat plates, after cultivating 24h in 37 ± 1 DEG C, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking is single Bacterium colony MRS is cultivated based on 37 ± 1 DEG C of culture 24h, conservation;
B. resistance to peracid:MRS culture mediums are adjusted into pH to 2.0,3.0,4.0 with watery hydrochloric acid, after being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat on MRS culture medium flat plates, in After 37 ± 1 DEG C of culture 24h, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking single bacterium colony is cultivated with MRS and is based on 37 ± 1 DEG C of culture 24h, conservation.
Strain after 4 kinds of domestications of the above presses 12:3:8:1 ratio is well mixed, by the feed liquid after 3% addition sterilizing of material liquid volume In, it is sufficiently stirred for, ferment 21h at 34-38 DEG C, is fermentation termination to pH=3.5, obtains mango juice;
(5) mango juice, purified water, sucrose are pressed 10:16:2 ratio mixing, adds the vitamin of material liquid volume 0.04% C, is well mixed, homogeneous 2 times, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa, 125-135 5s is heated at DEG C sterilized, it is filling to obtain final product.
Comparative example 1
(1) mango is cleaned, dried, and in input boiling water, blanching 10 seconds is pulled out and is immediately placed in cooling in cold water, peeling;
(2) mango after peeling is pressed into solid-liquid ratio 1:08 ratio adds purified water, stirs, and is beaten 10-30min, Matter 2 times, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa;
(3) feed liquid after homogeneous is placed in fermentation tank, glucose, sucrose is separately added into by the 12% of material liquid volume, mixing is equal It is even, sterilizing;
(4) Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, lactobacillus paracasei are pressed 1:1:1:1 ratio mixing is equal It is even, in the feed liquid after being sterilized by 3% addition of material liquid volume, it is sufficiently stirred for, ferment 59h at 34-38 DEG C, is hair to pH=3.8 Ferment terminal, obtains mango juice;
(5) mango juice, purified water, sucrose are pressed 10:16:2 ratio mixing, adds the vitamin of material liquid volume 0.05% C, is well mixed, homogeneous 2 times, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa, 125-135 3s is heated at DEG C sterilized, it is filling to obtain final product.
Comparative example 2
(1) mango is cleaned, dried, and in input boiling water, blanching 5 seconds is pulled out and is immediately placed in cooling in cold water, peeling;
(2) mango after peeling is pressed into solid-liquid ratio 1:0.8 ratio adds purified water, stirs, and is beaten 10-30min, Matter 2 times, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa;
(3) feed liquid after homogeneous is placed in fermentation tank, glucose, sucrose is separately added into by the 10% of material liquid volume, mixing is equal It is even, sterilizing;
(4) Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, 4 kinds of strains of lactobacillus paracasei are tamed through following steps Used after screening:
A. it is resistance to high sugared:Addition glucose is 40%, 50%, 60% using the ratio of glucose, respectively in MRS culture mediums After being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat On MRS culture medium flat plates, after cultivating 24h in 37 ± 1 DEG C, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking is single Bacterium colony MRS is cultivated based on 37 ± 1 DEG C of culture 24h, conservation;
B. resistance to peracid:MRS culture mediums are adjusted into pH to 2.0,3.0,4.0 with watery hydrochloric acid, after being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat on MRS culture medium flat plates, in After 37 ± 1 DEG C of culture 24h, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking single bacterium colony is cultivated with MRS and is based on 37 ± 1 DEG C of culture 24h, conservation.
Strain after 4 kinds of domestications of the above presses 4:1:7:1 ratio is well mixed, by the feed liquid after 3% addition sterilizing of material liquid volume In, it is sufficiently stirred for, ferment 32h at 34-38 DEG C, is fermentation termination to pH=3.6, obtains mango juice;
(5) mango juice, purified water, sucrose are pressed 10:16:2 ratio mixing, adds the vitamin of material liquid volume 0.04% C, is well mixed, homogeneous 2 times, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa, 125-130 6s is heated at DEG C sterilized, it is filling to obtain final product.
Comparative example 3
(1) mango is cleaned, dried, and in input boiling water, blanching 8 seconds is pulled out and is immediately placed in cooling in cold water, peeling;
(2) mango after peeling is pressed into solid-liquid ratio 1:0.8 ratio adds purified water, stirs, and is beaten 10-30min, Matter 2 times, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa;
(3) feed liquid after homogeneous is placed in fermentation tank, glucose, sucrose is separately added into by the 10% of material liquid volume, mixing is equal It is even, sterilizing;
(4) Lactobacillus plantarum, lactobacillus acidophilus, 3 kinds of strains of Lactobacillus casei are used after following steps acclimation and screening:
A. it is resistance to high sugared:Addition glucose is 40%, 50%, 60% using the ratio of glucose, respectively in MRS culture mediums After being inoculated with above-mentioned 3 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat On MRS culture medium flat plates, after cultivating 24h in 37 ± 1 DEG C, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking is single Bacterium colony MRS is cultivated based on 37 ± 1 DEG C of culture 24h, conservation;
B. resistance to peracid:MRS culture mediums are adjusted into pH to 2.0,3.0,4.0 with watery hydrochloric acid, after being inoculated with above-mentioned 3 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat on MRS culture medium flat plates, in After 37 ± 1 DEG C of culture 24h, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking single bacterium colony is cultivated with MRS and is based on 37 ± 1 DEG C of culture 24h, conservation.
Strain after 3 kinds of domestications of the above presses 5:3:15 ratio is well mixed, in the feed liquid after being sterilized by 3% addition of material liquid volume, It is sufficiently stirred for, ferment 32h at 34-38 DEG C, is fermentation termination to pH=3.6, obtains mango juice;
(5) mango juice, purified water, sucrose are pressed 10:16:2 ratio mixing, adds the vitamin of material liquid volume 0.04% C, is well mixed, homogeneous 2 times, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa, 125-130 6s is heated at DEG C sterilized, it is filling to obtain final product.
Comparative example 4
(1) mango is cleaned, dried, and in input boiling water, blanching 6 seconds is pulled out and is immediately placed in cooling in cold water, peeling;
(2) mango after peeling is pressed into solid-liquid ratio 1:0.8 ratio adds purified water, stirs, and is beaten 10-30min, Matter 2 times, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa;
(3) feed liquid after homogeneous is placed in fermentation tank, glucose, sucrose is separately added into by the 10% of material liquid volume, mixing is equal It is even, sterilizing;
(4) Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, 4 kinds of strains of lactobacillus paracasei are tamed through following steps Used after screening:
A. it is resistance to high sugared:Addition glucose is 40%, 50%, 60% using the ratio of glucose, respectively in MRS culture mediums After being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat On MRS culture medium flat plates, after cultivating 24h in 37 ± 1 DEG C, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking is single Bacterium colony MRS is cultivated based on 37 ± 1 DEG C of culture 24h, conservation;
B. resistance to peracid:MRS culture mediums are adjusted into pH to 2.0,3.0,4.0 with watery hydrochloric acid, after being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat on MRS culture medium flat plates, in After 37 ± 1 DEG C of culture 24h, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking single bacterium colony is cultivated with MRS and is based on 37 ± 1 DEG C of culture 24h, conservation.
Strain after 4 kinds of domestications of the above presses 15:1:7:2 ratio is well mixed, by the feed liquid after 3% addition sterilizing of material liquid volume In, it is sufficiently stirred for, ferment 32h at 34-38 DEG C, is fermentation termination to pH=3.6, obtains mango juice;
(5) mango juice, purified water, sucrose are pressed 10:16:2 ratio mixing, adds the vitamin of material liquid volume 0.04% C, is well mixed, homogeneous 2 times, first time homogenization pressure 20-25Mpa, second homogenization pressure 35-40Mpa, 125-130 6s is heated at DEG C sterilized, it is filling to obtain final product.
In the development process of mango fermented beverage of the invention, inventor carries out sensory evaluation to final finished, asks panelist's ginseng With test evaluation, specific standards of grading are as follows:
The taste test standards of grading of table 1
10 panelists participate in this taste test altogether, and obatained score is as follows:
The taste test result of table 2
Lactobacillus plantarum is adapted to grow fermentation in the vegetable materials such as water fruits and vegetables, and produces various uniquenesses tempting fragrance and wind Taste material;The probiotics such as lactobacillus acidophilus, Lactobacillus casei, lactobacillus paracasei acid producing ability, ferment local-flavor have his own strong points, Tunning has certain suppression other varied bacteria growing effects.The mango juice protected from trial test experimental result, the present invention Fermented beverage, by Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, 4 kinds of strains of lactobacillus paracasei in proper proportions The composite bacteria for obtaining is fermented, and obtains the fermented beverage of good to eat nutrition.
, by after domestication, resulting composite bacteria fermentability is strong, acidproof resistance to sugar, the mango juice obtained by fermentation is more for these strains Plus bright, fragrant pleasant, sweet mouthfeel is fine and smooth, uniform not stratified.

Claims (11)

1. a kind of mango fermented beverage, it is characterised in that the fermented bacterium of addition is Lactobacillus plantarum, lactobacillus acidophilus, cheese The mixed bacteria of lactobacillus, lactobacillus paracasei composition, the ratio of the mixed bacteria is 8-12:1-4:7-10:1.
2. mango fermented beverage as claimed in claim 1, it is characterised in that be made up of following steps:
(1) mango is cleaned, dried, and in input boiling water, blanching 5-10 seconds, is pulled out and is immediately placed in cooling in cold water, peeling;
(2) by mango mashing, the homogeneous after peeling;
(3) feed liquid after homogeneous is placed in fermentation tank, glucose, sucrose is separately added into by the 8%-12% of material liquid volume, mixed Close uniform, sterilizing;
(4) Lactobacillus plantarum, lactobacillus acidophilus, Lactobacillus casei, lactobacillus paracasei are pressed into 8-12:1-4:7-10:1 ratio It is well mixed, in the feed liquid after being sterilized by the 1%-5% additions of material liquid volume, it is sufficiently stirred for, ferment, obtain mango juice;
(5) mango juice, purified water, sucrose are pressed into 10-12:16:The ratio mixing of 1-5, by the 0.03%-0.05% of material liquid volume Vitamin C is added, is well mixed, homogeneous, it is sterilized, it is filling to obtain final product.
3. mango fermented beverage as claimed in claim 2, it is characterised in that in the step (2), the mango after peeling By solid-liquid ratio 1:The ratio of 0.5-1 adds purified water, stirs, and is beaten 10-30min.
4. mango fermented beverage as claimed in claim 2, it is characterised in that in the step (2), homogenization cycles are 1-3 It is secondary, homogenization pressure 20-50Mpa.
5. mango fermented beverage as claimed in claim 4, it is characterised in that in the step (2), homogenization cycles are 2 Secondary, first time homogenization pressure is 20-25Mpa, and second homogenization pressure is 35-40Mpa.
6. mango fermented beverage as claimed in claim 2, it is characterised in that the Lactobacillus plantarum, lactobacillus acidophilus, dry Lactobacillus paracasei, 4 kinds of strains of lactobacillus paracasei are used after following steps acclimation and screening:
(1) resistance to sugar high:Addition glucose is 40%, 50%, 60% using the ratio of glucose in MRS culture mediums, After being inoculated with above-mentioned 4 kinds of strains respectively, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, apply It is distributed on MRS culture medium flat plates, after cultivating 24h in 37 ± 1 DEG C, takes the dilution level of bacterium colony number 30-300CFU/ wares, chooses Single bacterium colony MRS cultures are taken based on 37 ± 1 DEG C of culture 24h, conservation;
(2) resistance to peracid:MRS culture mediums are adjusted into pH to 2.0,3.0,4.0 with watery hydrochloric acid, after being inoculated with above-mentioned 4 kinds of strains, 37 ± 1 DEG C of culture 24h, then take 1ml and be diluted to 10-8, concentration 1ml after dilution is taken, coat on MRS culture medium flat plates, in After 37 ± 1 DEG C of culture 24h, the dilution level of bacterium colony number 30-300CFU/ wares is taken, picking single bacterium colony is cultivated with MRS and is based on 37 ± 1 DEG C of culture 24h, conservation.
7. mango fermented beverage as claimed in claim 2, it is characterised in that in the step (4), fermentation temperature is 34-38 DEG C, fermentation time is 18-48h, to stopping fermentation during pH=3.5-3.8.
8. mango fermented beverage as claimed in claim 2, it is characterised in that in the step (5), homogenization cycles are 1-3 It is secondary, homogenization pressure 20-40Mpa.
9. mango fermented beverage as claimed in claim 2, it is characterised in that in the step (5), homogenization cycles are 2 Secondary, first time homogenization pressure is 20-25Mpa, and second homogenization pressure is 35-40Mpa.
10. mango fermented beverage as claimed in claim 2, it is characterised in that in the step (5), sterilization mode is super High-temperature instantaneous sterilization, 3-6s is heated at 125-150 DEG C.
11. mango fermented beverages as claimed in claim 1 or 2, it is characterised in that Lactobacillus plantarum in the mixed bacteria, Lactobacillus acidophilus, Lactobacillus casei, the ratio of lactobacillus paracasei are 12:3:8:1.
CN201510928920.4A 2015-12-14 2015-12-14 A kind of mango fermented beverage and preparation method thereof Pending CN106858229A (en)

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CN108567086A (en) * 2017-07-19 2018-09-25 杭州娃哈哈科技有限公司 A kind of compound lactobacillus-fermencucumber mango juice beverage and preparation method thereof
CN107712530A (en) * 2017-11-21 2018-02-23 贵州省现代农业发展研究所 Mango fermented beverage and preparation method thereof
CN107821894A (en) * 2017-11-30 2018-03-23 广西天峨县果然美食品有限公司 A kind of preparation method of mango juice fermented beverage
CN108244434A (en) * 2018-02-01 2018-07-06 南昌大学 A kind of fermentation loquat pear beverage with relieving cough and moistening lung and preparation method thereof
CN109287912A (en) * 2018-11-20 2019-02-01 广西壮族自治区农业科学院农产品加工研究所 It is a kind of to improve antioxidative mango lactic acid drink preparation method
CN109287912B (en) * 2018-11-20 2022-04-26 广西壮族自治区农业科学院农产品加工研究所 Preparation method of mango lactic acid beverage with improved oxidation resistance
CN110521900A (en) * 2019-09-27 2019-12-03 江南大学 A kind of fermentation mango juice and preparation method thereof rich in active plant lactobacillus
CN115005395A (en) * 2022-04-18 2022-09-06 广州酒家集团利口福食品有限公司 Mango stuffing and preparation method thereof
CN115005395B (en) * 2022-04-18 2024-04-19 广州酒家集团利口福食品有限公司 Mango stuffing and preparation method thereof

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