CN106807463B - Micro-fluidic chip and the microlayer model generation device for applying it - Google Patents
Micro-fluidic chip and the microlayer model generation device for applying it Download PDFInfo
- Publication number
- CN106807463B CN106807463B CN201710053913.3A CN201710053913A CN106807463B CN 106807463 B CN106807463 B CN 106807463B CN 201710053913 A CN201710053913 A CN 201710053913A CN 106807463 B CN106807463 B CN 106807463B
- Authority
- CN
- China
- Prior art keywords
- channel
- micro
- fluidic chip
- microlayer model
- dispersed phase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
Abstract
The present invention provides a kind of micro-fluidic chip and using its microlayer model generation device.The T-shaped structure of the micro-fluidic chip, comprising: continuous phase import department is a straight-through portion of T-shaped structure, internal to form continuous phase channel；Dispersed phase import department is the by-pass portion of T-shaped structure, internal to form dispersed phase channel；And microlayer model outlet portion, it is another straight-through portion of T-shaped structure, it is internal to form microlayer model channel.Wherein, continuous phase channel, dispersed phase channel and microlayer model channel cross in the inside of micro-fluidic chip, in intersection close to the side in microlayer model channel, form tapered channel.There are the tapered channels of a small scale in the downstream of main channel by the present invention, can produce more shearing forces, when continuous phase flows through the tapered channel so as to readily generate 100 μm of microlayer models below.
Description
Technical field
Dress is generated the present invention relates to microfluidic art more particularly to a kind of micro-fluidic chip and using its microlayer model
It sets.
Background technique
Microlayer model (Droplet) is a kind of manipulation very low volume fluids to grow up on micro-fluidic chip in recent years
Technology, principle are: by two kinds of immiscible liquid, with one such for continuous phase, another kind is dispersed phase, continuous phase
After entering microchannel simultaneously with dispersed phase, under the action of microchannel, dispersed phase is with micro volume (10-16~10-11L) unit
Formal distribution forms a series of microlayer model of dispersions in continuous phase.The manipulation micro volume that microlayer model technology can be convenient
Drop is widely used in biology, and chemistry, the fields such as material are with a wide range of applications, wherein how convenient stable generation
Microlayer model is crucial.
Currently, most microlayer model generation devices are all the micro-fluidic chips of plane type.Fig. 1 and Fig. 2 is existing skill
The structural schematic diagram of the micro-fluidic chip of art 1 (CN 103386333A).Referring to Fig.1 and 2, the micro-fluidic chip is by PMMA
Substrate is prepared using laser engraving combination thermocompression bonding method.Wherein, be machined on cover plate layer A oily phase import a, water phase into
Mouth b and drop outlets h；Oily phase introduction passage c, drop formation chamber f and drop channel g are machined on fluid channel layer B；It is intermediate
The through-hole structure i of array is machined on layer C；Water phase introduction passage d and water phase storage chamber e are machined on fluid channel layer D；Base
Bottom E is bare substrate.The operating process of Mass production drop is as shown in schematic diagram 2 in above-mentioned micro-fluidic chip: using injection
It pumps and injects organic phase, such as mineral oil, hexadecane etc. into oily phase channel；It is injected simultaneously using syringe pump into water phase channel pure
The aqueous solution of water or sample, then the solution flows through water phase introduction passage into water phase storage chamber closed at one end, finally from battle array
The drop formation chamber on the through-hole structure of column toward upper layer is run, and under the action of oily phase, then disposably forms multiple water-in-oil types
Microlayer model, the quantity of drop depends on the quantity of micro through hole；The microlayer model ultimately produced, can from drop channel, outlet outflow
To use conduit or beaker etc. to carry out the collection of drop.
However, production method usually will include photoetching, wet etching, glass for above-mentioned microlayer model generation device
The assembly of glass pipe, hot pressing forming polymer etc..Photoetching and wet etching technique need expensive instrument and clean room and loaded down with trivial details
Step, common laboratory and company can not provide above-mentioned condition, therefore constrain the application of micro-fluidic microlayer model technology；Glass tube
Though the technology of assembly and hot pressing organic polymer does not need particularly expensive instrument, loaded down with trivial details hand-made step and production
Time seriously reduces chip manufacturing efficiency and production quality, so that micro-fluidic microlayer model technology is difficult to promote.In addition, above-mentioned skill
The chip of art production is all non-removable, once having Partial Blocking or damage, is difficult replacement and maintenance.In addition, above-mentioned skill
Art belongs to plane surface processing method, is difficult to manufacture three-dimensional structure, is based on this, the generation technique of drop is mainly the T of plane at present
Type microchannel and the burnt microchannel structure of copolymerization, which has limited the development of micro-fluidic microlayer model technology.
Nearly 2 years, the rise of 3D printing technique provided a kind of simple and convenient method for the production of micro-fluidic chip, and one
The micro-fluidic chip of a little 3D printings starts to be seen in report.It is simple and quick using 3D printing technique production chip, do not need valuableness
Equipment and environment, avoid complicated making step and opposite sky high cost, but due to uncured printing glue be difficult from
It being discharged in small channel, the microchannel size made currently with 3D printing technique is still bigger, so that generate therewith
Microlayer model size is also bigger, is unable to satisfy current demand, this causes the 3D printing technique having many advantages at present can not
It is widely used in the preparation of micro-fluidic microlayer model chip.
Summary of the invention
(1) technical problems to be solved
In order to solve or at least partly alleviate above-mentioned technical problem, the present invention provides a kind of micro-fluidic chip and applications
Its microlayer model generation device.
(2) technical solution
According to an aspect of the invention, there is provided a kind of micro-fluidic chip.The T-shaped structure of the micro-fluidic chip, comprising:
Continuous phase import department is a straight-through portion of T-shaped structure, internal to form continuous phase channel；Dispersed phase import department is T-shaped structure
By-pass portion, it is internal to form dispersed phase channel；And microlayer model outlet portion, it is another straight-through portion of T-shaped structure, internal shape
At microlayer model channel.Wherein, continuous phase channel, dispersed phase channel and microlayer model channel cross in the inside of micro-fluidic chip,
Intersection forms tapered channel close to the side in microlayer model channel.
According to another aspect of the present invention, a kind of microlayer model generation device is provided.The microlayer model generation device includes:
Tube-like piece and connector；And micro-fluidic chip as above；Wherein, it in tube-like piece insertion connector, is protruded by the connector
In the dispersed phase channel of micro-fluidic chip, tube-like piece, connector and micro-fluidic chip are separable, detachable structure.
(3) beneficial effect
It can be seen from the above technical proposal that micro-fluidic chip of the present invention and application its microlayer model generation device at least have
There is one of following beneficial effect:
(1) main channel downstream there are the tapered channel of a small scale, which, which can produce, more cuts
Shear force, so as to readily generate 100 μm of microlayer models below；
(2) by tapered channel will be arranged in micro-fluidic chip dispersed phase channel, so as to will be uncured in 3D printing
Photoresist be smoothly discharged, can smoothly print this small tapered channel；
(3) for microlayer model generation device, micro-fluidic chip, PEEK pipe connector and PEEK pipe are all independent removable
It unloads, so that entire microlayer model generating means are easily changed and safeguard, in addition, PEEK pipe and its connector and seal washer are all
The commercialized accessory of low cost easily obtained improves its commercial value to reduce the cost of microlayer model generation device.
Detailed description of the invention
Fig. 1 and Fig. 2 is the structural schematic diagram of the micro-fluidic chip of the prior art 1.
Fig. 3 A, Fig. 3 B, Fig. 3 C and Fig. 3 D are respectively the top view of microlayer model generation device according to embodiments of the present invention, left view
Figure, main view and right view.
Fig. 4 is diagrammatic cross-section of the microlayer model generation device shown in Fig. 3 A~Fig. 3 D along the face O-O.
Fig. 5 is the structural schematic diagram according to microlayer model generating means of the embodiment of the present invention.
Fig. 6 is the optical microscope photograph that microlayer model is generated using microlayer model generating means shown in Fig. 5.
[main element symbol description of the present invention]
10- micro-fluidic chip；
11- continuous phase import department；12- dispersed phase import department；13- microlayer model outlet portion；
A- continuous phase channel；
A0- continuous phase feeder connection；
B- dispersed phase channel；
B0- dispersed phase feeder connection；B1- pyramidal structure；Interconnecting piece in B2-；
C- microlayer model channel；
The outlet of C0- microlayer model；
Contraction channel in the main channel D1-；Tapered channel in the main channel D2-
20-PEEK pipe connector；
21- interconnecting piece；22 tapered portion
30-PEEK pipe；
40- sealing rubber ring.
Specific embodiment
Inventive concept of the invention is to provide a kind of micro-fluidic chip with 3 D stereo construction, can generate phase
To lesser microlayer model, and it can pass through the stereolithography apparatus method (Stereolithography in 3D printing technique
Appearance, abbreviation SLA) it prepares, to realize simple and convenient, low-cost production and easy to maintain.
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, and reference
Attached drawing, the present invention is described in more detail.
One, micro-fluidic chip
In first exemplary embodiment of the invention, a kind of micro-fluidic chip is provided.Fig. 3 A, Fig. 3 B, Fig. 3 C and
Fig. 3 D is respectively top view, left view, main view and the right view of micro-fluidic chip according to embodiments of the present invention.Fig. 4 is Fig. 3 A
Diagrammatic cross-section of the micro-fluidic chip shown in~Fig. 3 D along the face O-O.A~Fig. 3 D, Fig. 4 referring to figure 3., the micro-fluidic core of the present embodiment
The whole T-shaped structure of piece, comprising: continuous phase import department 11 is a straight-through portion of T-shaped structure, internal to form continuous phase channel
A；Dispersed phase import department 12 is a by-pass portion of T-shaped structure, internal to form dispersed phase channel B；Microlayer model outlet portion 13 is T
Another straight-through portion of shape structure, it is internal to form microlayer model channel C；Wherein, continuous phase channel A, dispersed phase channel B and microlayer model
Channel C crosses in the inside of micro-fluidic chip, in intersection close to the side of microlayer model channel C, forms a tapered channel.
Below in conjunction with attached drawing, each component part of the present embodiment micro-fluidic chip is described in detail respectively.
The present embodiment micro-fluidic chip is to be printed by inexpensive desktop grade 3D printer using SLA method, preparation step letter
Single, preparation cost is low, and required preparation condition can meet with cost common laboratory and company, to greatly expand miniflow
The application field for controlling chip, reduces the cost of manufacture of micro-fluidic chip.
Referring to figure 4., the whole T-shaped structure of the present embodiment micro-fluidic chip, comprising: continuous phase import department 11, dispersed phase
Import department 12 and microlayer model outlet portion 13.Wherein, continuous phase import department 11 and microlayer model outlet portion 13 lead directly to for the two of T-shaped structure
Portion, dispersed phase import department 12 are the by-pass portion of T-shaped structure.
Continuous phase channel A is formed in continuous phase import department 11, in the origination side of continuous phase channel A, is had and is continuously communicated
Road entrance A0, for inputting continuous phase liquid.Dispersed phase channel B is formed inside dispersed phase import department 12, in dispersed phase channel B
Origination side, have dispersed phase feeder connection B0, for inputting dispersion fluid.Microlayer model is formed in microlayer model outlet portion 13
Channel C there is microlayer model to export C0, for exporting microlayer model in the termination side of microlayer model channel C.Wherein, continuous phase channel A,
Dispersed phase channel B and microlayer model channel C are that cross section is rectangular channel.
Inside micro-fluidic chip, continuous phase channel A and the setting of microlayer model channel C face constitute the master of micro-fluidic chip
Channel.Dispersed phase channel B is formed inside dispersed phase import department 12.Using dispersed phase channel B as boundary, main channel is in continuous phase import
The part of portion side is known as the upstream of main channel, and main channel is known as the downstream of main channel in the part of microlayer model import department side.
Intersection in main channel and dispersed phase channel B, the internal diameter of main channel narrow, this part, which is formed, shrinks channel D1.
In the downstream for shrinking channel D1 there are the tapered channel D2 of a small scale, internal diameter is become larger, and is gradually recovered into and is led to main
The identical size of upstream internal diameter in road.In the upstream side of tapered channel D2, dispersed phase channel B is connected in main channel.
It is understood that injecting continuous phase liquid by continuous phase channel A, dispersed phase is injected by dispersed phase channel B
Liquid, continuous phase liquid shrink channel and small tapered channel by this, can produce more shearing forces, so as to be easy
100 μm of microlayer models below of generation, this is just consistent with microlayer model size caused by traditional microfluidic microlayer model chip, can be with
Meets the needs of major part is to microlayer model at present, breaching can be generated using micro-fluidic chip prepared by 3D printing technique
Microlayer model the limit.
In the present embodiment, other than shrinking channel D1 and tapered channel D2, the internal diameter of main channel is between 800 μm~1000
Between μm.The internal diameter of channel D1 is shunk between 200 μm~300 μm.The internal diameter of dispersed phase channel B is between 170 μm~190 μm
Between.The subtended angle that the vertical section of tapered channel D2 is in is between 30 °~60 °.It, can using the present embodiment micro-fluidic chip
To generate the microlayer model that minimum dimension is 30 μm or so.
Referring to figure 4., in the present embodiment, dispersed phase channel B points are two parts: the interior interconnecting piece B2 of upstream side and downstream side
Tapered pyramidal structure B1.Internal screw thread is arranged in the inside of interior interconnecting piece B2, for the interconnecting piece with PEEK pipe connector
External screw thread matching.Match for the conical section at the top of tapered channel B1, with PEEK pipe connector.
It should be strongly noted that for pyramidal structure B1, in addition to being used to place connector, additionally provide one it is important
Function is as uncured printing glue passing away.Just because of the presence of this pyramidal structure B1, so that in this pyramidal structure
Uncured printing glue in the small tapered channel of B1 design can be smoothly discharged, small so as to smoothly print this
Tapered channel D2.The design structure proposed using the present embodiment, printable small tapered channel size is current desktop grade 3D
The half of the printable minimum channel size of printer.
It will be apparent to those skilled in the art that the 3D printing material of production the present embodiment micro-fluidic chip can be various types of
The photosensitive resin of type, the photosensitive resin preferably to match with 3D printer used.
Two, microlayer model generating means
Based on above-mentioned micro-fluidic chip, the present invention also provides a kind of microlayer model generating means.
In an exemplary embodiment of the present invention, a kind of microlayer model generating means are additionally provided.Fig. 5 is according to this hair
The structural schematic diagram of bright embodiment microlayer model generating means.As shown in figure 5, the present embodiment microlayer model generating means include: above-mentioned
(Polyetheretherketone, the polyether-ether-ketone) pipe connector 20 of micro-fluidic chip 10, PEEK described in embodiment and PEEK
Pipe 30.Wherein, PEEK pipe 30 is inserted into PEEK pipe connector 20, protrudes into micro-fluidic chip 10 by the PEEK pipe connector 20
In the B of dispersed phase channel.It is experimentally confirmed, the PEEK of insertion is managed it is also ensured that good concentricity.
Please continue to refer to Fig. 5, PEEK pipe connector 20 includes: with externally threaded outer interconnecting piece 21 and tapered taper
Portion 22.Wherein, the internal screw thread phase of the interior interconnecting piece B2 in the dispersed phase channel of the external screw thread and micro-fluidic chip of outer interconnecting piece 21
Match；The shape substantial match of the pyramidal structure B1 in the dispersed phase channel of tapered portion 22 and micro-fluidic chip.In practical set,
PEEK pipe connector 20 is inserted into the dispersed phase channel B of micro-fluidic chip, is connected by the matching between screw thread.
Please continue to refer to Fig. 5, PEEK pipe 30 is inserted into PEEK pipe connector 20.In order to improve PEEK pipe connector and miniflow
Control the sealing of chip, the present embodiment microlayer model generating means further include: be extruded in the outer wall of the tapered portion 22 of PEEK pipe connector
Sealing rubber ring 40 between the inner wall of the tapered channel B1 of the dispersed phase channel B of the micro-fluidic chip in outside.Gasket is added
Circle can be further improved the leakproofness of micro-fluidic chip.The connection type of screw thread encryption seal can guarantee dispersion fluid not
Leakage.
It can be seen that in the present embodiment microlayer model generating means, micro-fluidic chip 10,20 and of PEEK pipe connector
PEEK pipe 30 is all separate removable, so that entire microlayer model generating means are easily changed and safeguard.Furthermore, it is to be understood that
It is that in the present embodiment, PEEK pipe and its connector and seal washer are all the low cost commercialization accessories easily obtained, thus significantly
Reduce the cost of microlayer model generation device.
As shown in Figures 4 and 5, the internal screw thread that microlayer model generation device provided by the present invention provides adaptation is connect with PEEK pipe
Head cooperation, and reserve cavity space and place connector, and connector is connect with PEEK pipe.In this way, so that PEEK
Pipe is combined with 3D printing chip, so that dispersed phase imports main channel by PEEK pipe, the continuous phase for being carried out autonomous channel is squeezed,
Microlayer model is generated at PEEK tube head and chip wall.
In practical application, dispersed phase is managed by the PEEK that syringe pump is passed through adaptation, continuous phase by syringe pump by conduit and
Chip entrance is passed through main channel, and under the squeezing action of continuous phase, microlayer model produces at PEEK pipe nozzle and tapered micro-tunnel
It is raw.Finally, microlayer model is flowed out from main channel downstream, outlet, it may be convenient to be collected to microlayer model.
In order to verify the actual effect of the present embodiment microlayer model generation device, using dispersion fluid as deionized water, continuously
Phase liquid is that silicone oil is verified.Fig. 6 is the optical microphotograph that Water-In-Oil microlayer model is generated using microlayer model generating means shown in Fig. 5
Mirror photo.As can be seen that can smoothly generate less than very much 100 μm of Water-In-Oil using the microlayer model generation device of the present embodiment
Microlayer model.
So far, attached drawing is had been combined various embodiments of the present invention are described in detail.According to above description, this field
Technical staff should have clear understanding to micro-fluidic chip of the present invention and using its microlayer model generation device.
It should be noted that in attached drawing or specification text, the implementation for not being painted or describing is affiliated technology
Form known to a person of ordinary skill in the art, is not described in detail in field.In addition, the above-mentioned definition to each element and method is simultaneously
It is not limited only to various specific structures, shape or the mode mentioned in embodiment, those of ordinary skill in the art can carry out letter to it
It singly changes or replaces, such as:
(1) material for preparing micro-fluidic chip can also be other materials in addition to material listed above, preferably at
Ripe 3D printing material；
(2) other than it can generate Water-In-Oil or oil-in-water microlayer model, other kinds of micro- liquid can also be generated
Drop；
(3) other than square duct, continuous phase channel A, dispersed phase channel B and microlayer model channel C can also be round
The either channel of other cross section types；
(4) PEEK pipe and its connector can also be replaced with the pipe and connector of other types and material, inlet and outlet knot
Structure can also be replaced using other structures, such as: Rule head and threaded connector.
It should also be noted that, can provide the demonstration of the parameter comprising particular value herein, but these parameters are without definite etc.
In corresponding value, but analog value can be similar in acceptable error margin or design constraint.The side mentioned in embodiment
It is only the direction with reference to attached drawing to term, such as "upper", "lower", "front", "rear", "left", "right" etc., is not used to limit this
The protection scope of invention.Furthermore above-described embodiment can based on the considerations of design and reliability, be mixed with each other collocation use or and its
He uses embodiment mix and match, i.e., the technical characteristic in different embodiments can freely form more embodiments.
In conclusion the present invention devises the 3D that the novel microfluidic chip structure of one kind is used for micro-fluidic microlayer model chip
Printing preparation, solves the technical problem of the micro-fluidic microlayer model chip manufacturing of 3D printing, breaching desktop grade 3D printer can
The limit of the minimum dimension microchannel of printing.The micro-fluidic chip that its is proposed on this basis and the application micro-fluidic chip it is micro-
Dropproducing devices have stronger practical value.
Particular embodiments described above has carried out further in detail the purpose of the present invention, technical scheme and beneficial effects
It describes in detail bright, it should be understood that the above is only a specific embodiment of the present invention, is not intended to restrict the invention, it is all
Within the spirit and principles in the present invention, any modification, equivalent substitution, improvement and etc. done should be included in guarantor of the invention
Within the scope of shield.
Claims (10)
1. a kind of micro-fluidic chip, which is characterized in that T-shaped structure, comprising:
Continuous phase import department is a straight-through portion of T-shaped structure, internal to form continuous phase channel；
Dispersed phase import department is the by-pass portion of T-shaped structure, internal to form dispersed phase channel, and the dispersed phase channel is located at perpendicular
Histogram to；And
Microlayer model outlet portion is another straight-through portion of T-shaped structure, internal to form microlayer model channel；
Wherein, the continuous phase channel, dispersed phase channel and microlayer model channel cross in the inside of micro-fluidic chip, in intersection
Close to the side in microlayer model channel, tapered channel is formed, the tapered channel becomes larger towards downstream direction, internal diameter.
2. micro-fluidic chip according to claim 1, which is characterized in that the continuous phase channel and microlayer model channel face
Setting, constitutes the main channel of micro-fluidic chip；
The internal diameter of intersection in the main channel and dispersed phase channel, main channel narrows, and is formed and shrinks channel, is shrinking channel
Downstream form the tapered channel, the internal diameter of the tapered channel becomes larger, and is gradually recovered into and main channel upstream internal diameter phase
Same size.
3. micro-fluidic chip according to claim 2, which is characterized in that the subtended angle that the vertical section of the tapered channel is in
Between 30 °~60 °.
4. micro-fluidic chip according to claim 2, which is characterized in that the cross section of the main channel and dispersed phase channel
It is round or rectangular, in which:
The internal diameter in the dispersed phase channel is between 170 μm~190 μm；
In the main channel, the internal diameter in channel is shunk between 200 μm~300 μm；In addition to shrinking channel and tapered channel
Part internal diameter between 800 μm~1000 μm.
5. micro-fluidic chip according to claim 1, which is characterized in that the dispersed phase channel includes:
Internal screw thread is arranged in the interior interconnecting piece of upstream side, inside；And
The pyramidal structure in downstream side is connected to the interior interconnecting piece.
6. micro-fluidic chip according to any one of claim 1 to 5, which is characterized in that the micro-fluidic chip is by 3D
Prepared by the stereolithography apparatus method of printing technique, material is photosensitive resin.
7. a kind of microlayer model generation device characterized by comprising
Tube-like piece and connector；And
Micro-fluidic chip as described in claim 1；
Wherein, the tube-like piece is inserted into the connector, is communicated by the dispersion that the connector protrudes into the micro-fluidic chip
In road, the tube-like piece, connector and micro-fluidic chip are separable, detachable structure.
8. microlayer model generation device according to claim 7, which is characterized in that the tube-like piece is PEEK pipe, the company
Connector is PEEK pipe connector.
9. microlayer model generation device according to claim 8, it is characterised in that:
In the micro-fluidic chip, dispersed phase channel includes: the interior interconnecting piece of upstream side, and internal screw thread is arranged in inside；Under and
The pyramidal structure for swimming side, is connected to the interior interconnecting piece；
The PEEK pipe connector includes: with externally threaded outer interconnecting piece and tapered tapered portion, wherein the outer connection
The internal screw thread of the interior interconnecting piece in the dispersed phase channel of the external screw thread and micro-fluidic chip in portion matches；Tapered portion and micro-fluidic chip
Dispersed phase channel pyramidal structure shape matching；
Wherein, in the dispersed phase channel of the PEEK pipe connector insertion micro-fluidic chip, the PEEK pipe connector and miniflow
It controls chip and passes through the matching connection between screw thread.
10. microlayer model generation device according to claim 9, which is characterized in that further include:
Sealing rubber ring is extruded in the dispersed phase channel of the outer wall of the tapered portion of PEEK pipe connector and the micro-fluidic chip in outside
Between the inner wall of pyramidal structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710053913.3A CN106807463B (en) | 2017-01-22 | 2017-01-22 | Micro-fluidic chip and the microlayer model generation device for applying it |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710053913.3A CN106807463B (en) | 2017-01-22 | 2017-01-22 | Micro-fluidic chip and the microlayer model generation device for applying it |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106807463A CN106807463A (en) | 2017-06-09 |
| CN106807463B true CN106807463B (en) | 2019-09-24 |
Family
ID=59111642
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710053913.3A Active CN106807463B (en) | 2017-01-22 | 2017-01-22 | Micro-fluidic chip and the microlayer model generation device for applying it |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106807463B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107597221A (en) * | 2017-09-30 | 2018-01-19 | 深圳市博瑞生物科技有限公司 | Drop formation device and drop formation system |
| CN108535239B (en) * | 2018-03-28 | 2021-05-25 | 上海艾瑞德生物科技有限公司 | Micro-fluidic chip and detection system based on micro-droplets |
| CN110355991A (en) * | 2018-04-09 | 2019-10-22 | 北京大学 | 3D printing method and 3D printing equipment |
| CN108704678A (en) * | 2018-05-04 | 2018-10-26 | 上海理工大学 | The T-type micro flow control chip device that Morse code generates |
| CN108579830A (en) * | 2018-05-04 | 2018-09-28 | 上海理工大学 | The cross type micro flow control chip device that Morse code generates |
| CN109395788B (en) * | 2018-11-28 | 2024-04-09 | 西安交通大学 | Chip device for preparing liquid drops in tube |
| CN111514951A (en) * | 2020-05-19 | 2020-08-11 | 南京鼓楼医院 | Micro-fluidic chip prepared from micro-array type emulsion |
| CN112742071B (en) * | 2021-01-13 | 2022-07-05 | 西南科技大学 | Low surface energy droplet oriented transportation and separation structure |
| CN115475668B (en) * | 2022-08-22 | 2023-07-07 | 湖北师范大学 | Device and method for synthesizing morphology-controllable nano silver |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103165389A (en) * | 2011-12-19 | 2013-06-19 | 中国科学院大连化学物理研究所 | Sample introduction device |
| CN106140340A (en) * | 2016-08-19 | 2016-11-23 | 北京工业大学 | Micro-fluidic chip based on flow focusing type microchannel synthesis microemulsion drop |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015211931A (en) * | 2014-05-01 | 2015-11-26 | 学校法人同志社 | Method and apparatus for producing microdroplet using microfluidic chip |
-
2017
- 2017-01-22 CN CN201710053913.3A patent/CN106807463B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103165389A (en) * | 2011-12-19 | 2013-06-19 | 中国科学院大连化学物理研究所 | Sample introduction device |
| CN106140340A (en) * | 2016-08-19 | 2016-11-23 | 北京工业大学 | Micro-fluidic chip based on flow focusing type microchannel synthesis microemulsion drop |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106807463A (en) | 2017-06-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106807463B (en) | Micro-fluidic chip and the microlayer model generation device for applying it | |
| CN106140340B (en) | Micro-fluidic chip based on flow focusing type microchannel synthesis micro emulsion drop | |
| CN108671970B (en) | Method for generating double-size micro-droplets based on micro-fluidic chip | |
| CN102892704B (en) | For forming the device of drop in microfluidic circuit | |
| CN106975411B (en) | Micro-fluidic chip based on 3D prints and including emulsion generating device of this chip | |
| CN105524829A (en) | Micro-fluidic chip for manufacturing tissue engineering micromodule | |
| Song et al. | Fabrication of 3D printed modular microfluidic system for generating and manipulating complex emulsion droplets | |
| CN106669556B (en) | A method of millimeter grade particles are prepared using transformation microfluidic channel | |
| CN107519958A (en) | A kind of Microfluidic droplet generating means and its application | |
| CN105921188A (en) | Microfluidic device capable of rapidly preparing emulsion in multiple dimensions | |
| CN106215986A (en) | A kind of PDMS microfluidic chip structure and preparation method thereof | |
| CN112892630B (en) | Multi-material liquid drop generation system based on coaxial flow focusing structure | |
| CN105498875A (en) | Centrifugal micro-fluidic chip for preparing liquid drops | |
| CN101486437B (en) | Micro-nozzle and manufacture method thereof | |
| Lian et al. | Microfluidic formation of highly monodispersed multiple cored droplets using needle‐based system in parallel mode | |
| CN103861535A (en) | Single-dispersity and opposite-polarity Janus gel microsphere with core-shell structure, preparation method and microfluid control device used | |
| CN108405003A (en) | A kind of micro-fluidic chip and application method of the double emulsion droplets shells of stripping | |
| CN105413779B (en) | Prepare the micro-fluidic chip clamp of multistage emulsion and the preparation system of multistage emulsion | |
| CN108525622A (en) | A kind of reaction of high order micro fluidic device and its prepare applications to nanostructures | |
| CN109351369B (en) | Microfluidic droplet generation chip | |
| CN104741049A (en) | Microsphere manufacturing device | |
| CN107890785B (en) | Electrofusion preparation device and control method for multi-component liquid drops | |
| Liu et al. | Enhanced droplet formation in a T-junction microchannel using electric field: A lattice Boltzmann study | |
| CN205133627U (en) | Structure is made to pretreatment fluid of organizational project micromodule | |
| CN208407024U (en) | A kind of micro-fluidic double emulsion drop formation device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |