CN106771153A - It is a kind of for feed, the vomitoxin colloidal-gold detecting-card of feedstuff - Google Patents
It is a kind of for feed, the vomitoxin colloidal-gold detecting-card of feedstuff Download PDFInfo
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- CN106771153A CN106771153A CN201611020362.2A CN201611020362A CN106771153A CN 106771153 A CN106771153 A CN 106771153A CN 201611020362 A CN201611020362 A CN 201611020362A CN 106771153 A CN106771153 A CN 106771153A
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- pad
- vomitoxin
- nitrocellulose filter
- plastic casing
- colloidal
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- Hematology (AREA)
- Urology & Nephrology (AREA)
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- Food Science & Technology (AREA)
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Abstract
It is used for feed the invention discloses one kind, the vomitoxin colloidal-gold detecting-card of feedstuff, including plastic casing, sample pad, pad, nitrocellulose filter, absorption pad and base plate, the plastic casing fixes base plate by draw-in groove, the plastic casing lower section and base plate top are fixed with depression bar, the depression bar is pressed in sample pad, pad, nitrocellulose filter, absorption pad top, one end is provided with adding mouth on the plastic casing, the upper middle part of the plastic casing is provided with observation window, adding mouth sample pad corresponding with observation window lower section and nitrocellulose filter, the sample pad is connected with pad adjacent overlap joint, the pad and the overlap joint connection of nitrocellulose filter adjacent, the nitrocellulose filter and the overlap joint connection of absorption pad adjacent.This kind is used for feed, the vomitoxin colloidal-gold detecting-card simple structure of feedstuff, and detection is accurate.
Description
Technical field
It is specially a kind of to be vomitted for feed, feedstuff the present invention relates to vomitoxin dot immune gold filtration assay field
Tell toxin colloidal-gold detecting-card.
Background technology
Vomitoxin (vomitoxin), also known as deoxynivalenol (DON), chemistry entitled 3 α, 7 α, 15 1
Trihydroxy grass Fusariumsp -9- alkene -8- ketone, belongs to trichothecene.Gained the name because it can cause the vomiting of pig, it is right
Human body has certain damaging effect, and European Union's criteria for classification is three-level carcinogenic substance.
Telling toxin has very strong toxicity to humans and animals, can cause humans and animals vomiting, diarrhoea, skin irritatin, food refusal,
Neurological disorders, miscarriage, stillborn foetus etc., pig are the animals most sensitive to vomitoxin, and poultry takes second place, and ruminant is due to the micro- life of cud
The effect of thing, tolerance is most strong.For store pig, 10 ~ 20min after the feed feeding containing 12 ~ 14g/t vomitoxins
Vomiting, abnormal anxiety and phenomenon of grinding one's teeth in sleep occurs, more than toxic amount 19g/t is complete food refusal.Research shows, not of the same race
The LD50 of class animal（mg/kg）For：The intraperitoneal injection 70.0 of its mouse oral 46.8；Neonate rat oral 7.3；Rat footpath mouthful 7.3；North
Capital duckling hypodermic injection 27.0；And nauseant amount is：Beijing duckling hypodermic injection 10.0；Dog hypodermic injection 0.1 oral 0.1；Pig
Intraperitoneal injection 0.05；Pigeon oral 10.0.
There are thin-layered chromatography, high-efficient liquid phase technique, ELISA, glue currently for the detection method of vomitoxin in feed
Body gold rapid test piece method and fluorescence rapid test piece method.Wherein thin layer color chromatography and high performance liquid chromatography are used as GB side
Method, as a result accurately, but needs to use the instrument of costliness, it is necessary to professional's operation, complex operation, time-consuming, is unsuitable in base
Layer is promoted the use of.
The content of the invention
It is an object of the invention to provide a kind of for feed, the vomitoxin colloidal-gold detecting-card of feedstuff, possess
Vomitoxin in detection feed, solve feed the resting period it is long generate vomitoxin and do not detect made to production and living
Into the problem of loss.
To achieve the above object, the present invention provides following technical scheme：
It is a kind of for feed, the vomitoxin colloidal-gold detecting-card of feedstuff, including plastic casing, sample pad, pad, nitre
Acid cellulose film, absorption pad and base plate, the plastic casing fix base plate by draw-in groove, on the plastic casing lower section and base plate
Side is fixed with depression bar, and the depression bar is pressed in sample pad, pad, nitrocellulose filter, absorption pad top, the plastic casing
Upper one end is provided with adding mouth, and the upper middle part of the plastic casing is provided with observation window, adding mouth sample corresponding with observation window lower section
This pad and nitrocellulose filter, the sample pad and pad adjacent overlap joint are connected, the pad and nitrocellulose filter
Adjacent overlap joint connection, the nitrocellulose filter and the overlap joint connection of absorption pad adjacent.
Preferably, on the nitrocellulose filter on be fixed with detection line and control line.
Preferably, vomitoxin coupled antigen is coated with the detection line, anti-mouse secondary antibody is coated with the control line,
Emesis toxin mouse monoclonal antibody-colloidal gold conjugate is coated with the pad, anti-mouse secondary antibody is sheep anti-mouse igg antibody or rabbit-anti
Mouse IgG antibody.
Preferably, the width of the well and observation window is less than sample pad and the width of nitrocellulose filter
Preferably, the plastic casing and base plate are PVC material and are made.
Compared with prior art, beneficial effects of the present invention are as follows：This kind of feed, the vomitoxin collaurum of feedstuff
Detection card, easy to operate, result interpretation is easily, time-consuming short, as rapid screening method, it is easy to promoted the use of in basic unit.
Brief description of the drawings
Fig. 1 is that the present invention is a kind of for feed, the vomitoxin colloidal-gold detecting-card structural representation of feedstuff.
In figure：1- plastic casings, 2- depression bars, 3- samples hole, 4- sample pads, 5- pads, 6- detection lines, 7- nitrocellulose
Film, 8- control lines, 9- observation windows, 10- absorption pads, 11- base plates.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Fig. 1 is referred to, the present invention provides a kind of technical scheme：It is a kind of for feed, the vomitoxin colloid of feedstuff
Gold detection card, including plastic casing 1, sample pad 4, pad 5, nitrocellulose filter 7, absorption pad 10 and base plate 11, the modeling
Material shell 1 fixes base plate 11 by draw-in groove, and the lower section of the plastic casing 1 and the top of base plate 11 are fixed with depression bar 2, the depression bar
2 are pressed in sample pad 4, pad 5, nitrocellulose filter 7, the top of absorption pad 10, and one end is provided with adding mouth on the plastic casing 1
3, the upper middle part of the plastic casing 1 is provided with observation window 9, the sample pad 4 corresponding with the lower section of observation window 9 of the adding mouth 3 and nitric acid
Cellulose membrane 7, the sample pad 4 and the adjacent of pad 5 overlap joint is connected, the pad 5 and the adjacent of nitrocellulose filter 7
Overlap joint connection, the nitrocellulose filter 7 and the overlap joint connection of the adjacent of absorption pad 10.
Operation principle：Sample is added in sample pad 4 first, and the HCG in sample is marked with the gold combined on gold colloid surface
Antibody is combined（Gold labeling antibody-HCG）And chromatography upwards together, flow through NC films 7 when, anti-HCG in detection line 6 with combine
The HCG for having colloid gold particle reacts to form sandwich complex, and rests on the position of detection line 6, uncombined gold labeling antibody
Continue flow forward, captured by sheep anti-mouse igg when flowing to 8 position of nature controlling line（The anti-gold labeling antibody of solid phase two）, rest on nature controlling line
Position.Remaining sample and golden label can all continue towards suction absorption pad 10.
After detection line 6 or nature controlling line 8 capture gold labeling antibody, the lines of red can be showed.If ' negative ' specimens, then
Only nature controlling line 8 develops the color, if positive sample, then detection line 6 develops the color simultaneously with nature controlling line 8
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, it is possible to understand that
These embodiments can be carried out with various changes, modification, replacement without departing from the principles and spirit of the present invention and become
Type, the scope of the present invention be defined by the appended.
Claims (5)
1. a kind of for feed, the vomitoxin colloidal-gold detecting-card of feedstuff, including plastic casing（1）, sample pad（4）、
Pad（5）, nitrocellulose filter（7）, absorption pad（10）And base plate（11）, it is characterised in that the plastic casing（1）Pass through
Draw-in groove fixes base plate（11）, the plastic casing（1）Lower section and base plate（11）Top is fixed with depression bar（2）, the depression bar（2）
It is pressed in sample pad（4）, pad（5）, nitrocellulose filter（7）, absorption pad（10）Top, the plastic casing（1）Upper one end
It is provided with adding mouth（3）, the plastic casing（1）Upper middle part be provided with observation window（9）, the adding mouth（3）And observation window（9）Under
Side's correspondence sample pad（4）And nitrocellulose filter（7）, the sample pad（4）With pad（5）Adjacent overlap joint connection, it is described
Pad（5）And nitrocellulose filter（7）Adjacent overlap joint connection, the nitrocellulose filter（7）And absorption pad（10）It is adjacent
Place's overlap joint connection.
2. according to claim 1 a kind of for feed, the vomitoxin colloidal-gold detecting-card of feedstuff, its feature exists
In：The nitrocellulose filter（7）It is upper to be fixed with detection line（6）And control line（8）.
3. according to claim 2 a kind of for feed, the vomitoxin colloidal-gold detecting-card of feedstuff, its feature exists
In：The detection line（6）On be coated with vomitoxin coupled antigen, the control line（8）On be coated with anti-mouse secondary antibody, the knot
Close pad（5）On be coated with emesis toxin mouse monoclonal antibody-colloidal gold conjugate, anti-mouse secondary antibody is sheep anti-mouse igg antibody or rabbit-anti mouse
IgG antibody.
4. according to claim 1 a kind of for feed, the vomitoxin colloidal-gold detecting-card of feedstuff, its feature exists
In：The well（3）And observation window（9）Width be less than sample pad（4）And nitrocellulose filter（7）Width.
5. according to claim 1 a kind of for feed, the vomitoxin colloidal-gold detecting-card of feedstuff, its feature exists
In：The plastic casing（1）And base plate（11）PVC material is to be made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611020362.2A CN106771153A (en) | 2016-11-21 | 2016-11-21 | It is a kind of for feed, the vomitoxin colloidal-gold detecting-card of feedstuff |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611020362.2A CN106771153A (en) | 2016-11-21 | 2016-11-21 | It is a kind of for feed, the vomitoxin colloidal-gold detecting-card of feedstuff |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106771153A true CN106771153A (en) | 2017-05-31 |
Family
ID=58969631
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611020362.2A Pending CN106771153A (en) | 2016-11-21 | 2016-11-21 | It is a kind of for feed, the vomitoxin colloidal-gold detecting-card of feedstuff |
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| CN (1) | CN106771153A (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999032886A1 (en) * | 1997-12-23 | 1999-07-01 | Vicam, L.P. | Generic signalling mechanism for detection of analytes |
| CN202471718U (en) * | 2012-01-16 | 2012-10-03 | 青岛汉唐生物科技有限公司 | Clenbuterol hydrochloride detection kit |
| CN202710564U (en) * | 2012-06-15 | 2013-01-30 | 北京博欧实德生物技术有限公司 | Kit for fast detecting vomitoxin |
| CN203148951U (en) * | 2012-12-04 | 2013-08-21 | 杭州南开日新生物技术有限公司 | Colloidal gold reagent plate for rapid detection of vomitoxin in forage |
| CN203643453U (en) * | 2013-11-29 | 2014-06-11 | 刘宏书 | Biological rapid detection test card |
| CN204044161U (en) * | 2014-07-31 | 2014-12-24 | 北京康源泰博生物科技有限公司 | Deoxynivalenol enol colloidal gold reagent plate in a kind of grain and oil of detection fast |
| CN104345145A (en) * | 2013-07-31 | 2015-02-11 | 北京勤邦生物技术有限公司 | Test strip for detecting vomitoxin and applications thereof |
| CN204405673U (en) * | 2015-02-13 | 2015-06-17 | 北京中检安泰诊断科技有限公司 | For placing the kit of colloidal gold strip |
| CN104865381A (en) * | 2015-04-22 | 2015-08-26 | 河南省康星药业股份有限公司 | Pig early pregnancy factor colloidal gold test paper card and preparation method thereof |
| CN204903547U (en) * | 2015-08-13 | 2015-12-23 | 杭州中翰盛泰生物技术有限公司 | Reaction plate |
| CN205333646U (en) * | 2016-01-19 | 2016-06-22 | 无锡中德伯尔生物技术有限公司 | A vomitoxin colloidal gold detects and blocks for fodder, feed ingredient |
| CN205388606U (en) * | 2016-02-04 | 2016-07-20 | 广州市微米生物科技有限公司 | Anti -return colloidal gold chromatography detects card |
-
2016
- 2016-11-21 CN CN201611020362.2A patent/CN106771153A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999032886A1 (en) * | 1997-12-23 | 1999-07-01 | Vicam, L.P. | Generic signalling mechanism for detection of analytes |
| CN202471718U (en) * | 2012-01-16 | 2012-10-03 | 青岛汉唐生物科技有限公司 | Clenbuterol hydrochloride detection kit |
| CN202710564U (en) * | 2012-06-15 | 2013-01-30 | 北京博欧实德生物技术有限公司 | Kit for fast detecting vomitoxin |
| CN203148951U (en) * | 2012-12-04 | 2013-08-21 | 杭州南开日新生物技术有限公司 | Colloidal gold reagent plate for rapid detection of vomitoxin in forage |
| CN104345145A (en) * | 2013-07-31 | 2015-02-11 | 北京勤邦生物技术有限公司 | Test strip for detecting vomitoxin and applications thereof |
| CN203643453U (en) * | 2013-11-29 | 2014-06-11 | 刘宏书 | Biological rapid detection test card |
| CN204044161U (en) * | 2014-07-31 | 2014-12-24 | 北京康源泰博生物科技有限公司 | Deoxynivalenol enol colloidal gold reagent plate in a kind of grain and oil of detection fast |
| CN204405673U (en) * | 2015-02-13 | 2015-06-17 | 北京中检安泰诊断科技有限公司 | For placing the kit of colloidal gold strip |
| CN104865381A (en) * | 2015-04-22 | 2015-08-26 | 河南省康星药业股份有限公司 | Pig early pregnancy factor colloidal gold test paper card and preparation method thereof |
| CN204903547U (en) * | 2015-08-13 | 2015-12-23 | 杭州中翰盛泰生物技术有限公司 | Reaction plate |
| CN205333646U (en) * | 2016-01-19 | 2016-06-22 | 无锡中德伯尔生物技术有限公司 | A vomitoxin colloidal gold detects and blocks for fodder, feed ingredient |
| CN205388606U (en) * | 2016-02-04 | 2016-07-20 | 广州市微米生物科技有限公司 | Anti -return colloidal gold chromatography detects card |
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Application publication date: 20170531 |
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