CN106754811B - 一种突变型Tn5转座酶及其制备方法和应用 - Google Patents
一种突变型Tn5转座酶及其制备方法和应用 Download PDFInfo
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- CN106754811B CN106754811B CN201611196754.4A CN201611196754A CN106754811B CN 106754811 B CN106754811 B CN 106754811B CN 201611196754 A CN201611196754 A CN 201611196754A CN 106754811 B CN106754811 B CN 106754811B
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- C12N9/10—Transferases (2.)
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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CN201611196754.4A CN106754811B (zh) | 2016-12-21 | 2016-12-21 | 一种突变型Tn5转座酶及其制备方法和应用 |
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CN201611196754.4A CN106754811B (zh) | 2016-12-21 | 2016-12-21 | 一种突变型Tn5转座酶及其制备方法和应用 |
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CN106754811B true CN106754811B (zh) | 2019-05-14 |
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Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7402156B2 (ja) * | 2017-08-30 | 2023-12-20 | カパ バイオシステムズ, インコーポレイテッド | トランスポザーゼ組成物、製造方法およびスクリーニング方法 |
CN108265040B (zh) * | 2017-09-24 | 2021-02-09 | 天津强微特生物科技有限公司 | 一种嗜盐古细菌中的转座酶高活性突变体 |
CN107586835B (zh) * | 2017-10-19 | 2020-11-03 | 东南大学 | 一种基于单链接头的下一代测序文库的构建方法及其应用 |
CN109400714B (zh) * | 2018-10-26 | 2019-11-01 | 南京诺唯赞生物科技有限公司 | 抗体靶向的重组融合蛋白以及其在表观遗传学中的应用 |
JP2022515002A (ja) * | 2018-12-17 | 2022-02-17 | イルミナ インコーポレイテッド | 配列決定用ライブラリーを調製するための方法および手段 |
CN111041563B (zh) * | 2019-12-31 | 2023-07-21 | 广州精科医学检验所有限公司 | 一种靶向序列捕获及pcr建库方法 |
CN115948363B (zh) * | 2022-08-26 | 2024-02-27 | 武汉影子基因科技有限公司 | Tn5转座酶突变体及其制备方法和应用 |
CN115785283B (zh) * | 2022-11-02 | 2024-05-31 | 武汉影子基因科技有限公司 | PAG-Tn5突变体及其应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5965443A (en) * | 1996-09-09 | 1999-10-12 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
CN1251135A (zh) * | 1996-09-09 | 2000-04-19 | 威斯康星校友研究基金会 | 采用经修饰的tn5转座酶的体外转座*** |
CN1367840A (zh) * | 1999-08-02 | 2002-09-04 | 威斯康星校友研究基金会 | 突变型Tn5转座酶及其使用方法 |
WO2015160895A2 (en) * | 2014-04-15 | 2015-10-22 | Illumina, Inc. | Modified transposases for improved insertion sequence bias and increased dna input tolerance |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030143740A1 (en) * | 2001-10-15 | 2003-07-31 | Christine Wooddell | Processes for transposase mediated integration into mammalian cells |
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2016
- 2016-12-21 CN CN201611196754.4A patent/CN106754811B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5965443A (en) * | 1996-09-09 | 1999-10-12 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
CN1251135A (zh) * | 1996-09-09 | 2000-04-19 | 威斯康星校友研究基金会 | 采用经修饰的tn5转座酶的体外转座*** |
CN1367840A (zh) * | 1999-08-02 | 2002-09-04 | 威斯康星校友研究基金会 | 突变型Tn5转座酶及其使用方法 |
WO2015160895A2 (en) * | 2014-04-15 | 2015-10-22 | Illumina, Inc. | Modified transposases for improved insertion sequence bias and increased dna input tolerance |
Non-Patent Citations (6)
Title |
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SPECIFICITY OF TRANSPOSON TN5 INSERTION;DOUGLAS E. BERG 等;《Genetics》;19831231;第105卷;第813-828页 |
Tn5 Transposase Active Site Mutants;Todd A. Naumann 等;《THE JOURNAL OF BIOLOGICAL CHEMISTRY》;20020517;第277卷(第20期);第17623-17629页 |
Tn5 Transposase Active Site Mutations Suggest Position of Donor Backbone DNA in Synaptic Complex;Gregory Peterson 等;《THE JOURNAL OF BIOLOGICAL CHEMISTRY》;20030117;第278卷(第3期);第1904页右栏第2-3段,第1905页右栏第2-9段,第1906段左栏第1段 |
短小芽孢杆菌(Bacillus pumilus)Tn5转座突变体系的构建及抑菌筛选分析;胡晓璐;《中国学位论文全文数据库》;20150925;第1-79页 |
细菌转座子Tn5gusA5在野油菜黄单胞菌8004中转座规律分析及生物学验证;唐江涛;《中国优秀硕士学位论文全文数据库 基础科学辑》;20040315(第1期);A006-63 |
细菌转座子Tn5转座机理的研究进展;唐江涛 等;《广西农业生物科学》;20031231;第22卷(第4期);第316-321页 |
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Address after: Qixia District of Nanjing City, Jiangsu province 210038 Branch Road, Hongfeng Technology Park building C1-2 Patentee after: Nanjing novozan Biotechnology Co., Ltd Address before: Qixia District of Nanjing City, Jiangsu province 210038 Branch Road, Hongfeng Technology Park building C1-2 Patentee before: VAZYME BIOTECH (NANJING) Co.,Ltd. |