CN106754490A - A kind of culture medium for accelerating Thiobacillus thioxidans propagation - Google Patents
A kind of culture medium for accelerating Thiobacillus thioxidans propagation Download PDFInfo
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- CN106754490A CN106754490A CN201611098103.1A CN201611098103A CN106754490A CN 106754490 A CN106754490 A CN 106754490A CN 201611098103 A CN201611098103 A CN 201611098103A CN 106754490 A CN106754490 A CN 106754490A
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- thiobacillus thioxidans
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention provides a kind of culture medium for accelerating Thiobacillus thioxidans propagation.Thiobacillus thioxidans strain（Thiobacillusthiooxidans, T.t）From national Culture Collection, numbering is ATCC 53990 for purchase.Slow to solve the problems, such as existing Thiobacillus thioxidans growth rate in conventional medium, the present invention promotes the purpose of Thiobacillus thioxidans fast breeding by composite nutrient-fluid is added in conventional medium, reaching.Promotion Thiobacillus thioxidans fast breeding culture medium provided by the present invention can significantly promote Thiobacillus thioxidans growth rate compared with conventional medium, shorten incubation time.
Description
Technical field
The present invention relates to a kind of culture medium prescription for accelerating Thiobacillus thioxidans propagation, more particularly to one kind is added in routine
Culture medium and promote the nutrient solution of Thiobacillus thioxidans fast breeding.
Background technology
Thiobacillus thioxidans is a kind of mineral chemoautotrophic bacteria, obligate aerobic, acidophilus, Gram-negative, in shaft-like,
Wide 0.3~0.5 μm, Thiobacillus thioxidans obtains itself cell growth and metabolism with the sulfide of oxidizing simple substance sulphur or reduction-state
Required energy, with NH4 +It is nitrogen source, with CO in air2It is carbon source.Thiobacillus thioxidans be widely used in oil pipeline,
It is also in terms of microbial leaching, bacterial desulfurization to have application in oil storage tank and fuel tanker more.It has been found that sulfur oxide sulphur bar
Bacterium also has significant effect in terms of waste water control, soil remediation.In recent years, Thiobacillus thioxidans is in medicine, environmental protection, agriculture
The application of the aspects such as industry, bioenergy is paid attention to, many countries increase to the exploitation of Thiobacillus thioxidans microbial resources and
Utilize.
Thiobacillus thioxidans is a kind of obligate chemoautotrophic bacteria, and conventional culture medium has Waksman medium, Staeky
The conventional mediums such as medium, 9K-medium.But, with these conventional medium culture Thiobacillus thioxidans, its speed of growth
Slowly, it is difficult to reach the need for meeting preparation of industrialization.Therefore, the rapid culture techniques of Thiobacillus thioxidans microorganism enjoy pass
Note.At present, to how to accelerate Thiobacillus thioxidans propagation cultural method seldom studies have reported that.The present invention is by conventional training
Composite nutrient-fluid is added in foster base, these nutrient solutions participate in the growth metabolism process of Thiobacillus thioxidans and accelerate its growth and breeding
Speed, so as to solve the problems, such as that Thiobacillus thioxidans cellar culture growth rate is slow.
The content of the invention
It is an object of the invention to provide a kind of culture medium prescription for accelerating Thiobacillus thioxidans propagation.
A kind of culture medium prescription for accelerating Thiobacillus thioxidans propagation is realized according to the following steps.
A kind of culture medium that can accelerate Thiobacillus thioxidans propagation：Nutrient solution, the nutrition are added in conventional medium
Liquid is peptone and dusty yeast.The nutrient solution of described promotion Thiobacillus thioxidans fast breeding, is egg by weight percentage
The white addition of peptone and dusty yeast in conventional medium is 0.5%~1%.It is i.e. of the invention to accelerate Thiobacillus thioxidans increasing
The culture medium prescription grown is：Weight percentage, 0.2%（NH4）2SO4、 0.3% KH2PO4、0.05% MgSO4·7H2O、
0.001% FeSO4·7H2O、0.025% CaCl·2H2O, 1% Cosan, 0.5%~1% peptone and 0.5%~1% dusty yeast.
Preferably, being subject to percent by weight in a kind of above-mentioned culture medium that can accelerate Thiobacillus thioxidans propagation
It is 5%~10% brown sugar.
The present invention has bright as a kind of culture medium of promotion Thiobacillus thioxidans fast breeding compared with conventional medium
Aobvious promotes strain growth, shortens the effect of incubation time.Result using the medium culture Thiobacillus thioxidans shows,
In incubation, gradually sink and be dispersed in water with thalli growth, the sulphur powder particles for swimming in the water surface, solution is by clarifying
The muddy time is gradually become, compared with conventional medium, 5-7 days is advanced by, strain growth speed is significantly higher than cellar culture
Base.
Specific embodiment
Technical solution of the present invention is not limited to act specific embodiment set forth below, also including between each specific embodiment
Any combination.
Embodiment 1.
A kind of culture medium prescription of quickening Thiobacillus thioxidans propagation of present embodiment is realized according to the following steps.
Cellar culture based formulas：Weight percentage, 0.2%（NH4）2SO4、 0.3% KH2PO4、0.05%
MgSO4·7H2O、0.001% FeSO4·7H2O、0.025% CaCl·2H2O, 1% Cosan.
Add the culture medium prescription of nutrient solution：Weight percentage, 0.2%（NH4）2SO4、 0.3% KH2PO4、
0.05% MgSO4·7H2O、0.001% FeSO4·7H2O、0.025% CaCl·2H2O, 1% Cosan, 0.5% peptone and
0.5% dusty yeast.
It is control, training with conventional medium in addition peptone and the inoculation of medium Thiobacillus thioxidans of dusty yeast
The condition of supporting is 30 DEG C, 180rpm constant-temperature shaking cultures.Compared with conventional medium, the culture medium of addition peptone and dusty yeast,
Swim in the water surface Cosan particles sink and be dispersed in water, solution is changed into muddy from limpid, bacterial number increases, OD600Reach
To more than 2.8, compare with conventional medium, total number of bacteria is improved, time advance 5 days.Result shows, in conventional medium
Addition peptone and dusty yeast, can significantly improve the growth and breeding speed of Thiobacillus thioxidans, shorten incubation time, realize oxidation
The quick flourish of sulphur Thiobacillus.
Embodiment 2.
A kind of culture medium prescription of quickening Thiobacillus thioxidans propagation of present embodiment is realized according to the following steps.
Conventional medium is prepared：Weight percentage, 0.2%（NH4）2SO4、 0.3% KH2PO4、0.05%
MgSO4·7H2O、0.001% FeSO4·7H2O、0.025% CaCl·2H2O, 1% Cosan.
The culture medium for adding nutrient solution is prepared：Weight percentage, 0.2%（NH4）2SO4、 0.3% KH2PO4、
0.05% MgSO4·7H2O、0.001% FeSO4·7H2O、0.025% CaCl·2H2O, 1% Cosan, 0.5% peptone, 0.5%
Dusty yeast and 10% brown sugar.
It is control, 30 DEG C, 180rpm perseverances with conventional medium in the inoculation of medium Thiobacillus thioxidans of addition brown sugar
Warm shaken cultivation Thiobacillus thioxidans.Compared with conventional medium, the culture medium of brown sugar is added, swim in the Cosan of the water surface
Grain sinks and is dispersed in water, solution is changed into muddy from limpid, and solution is changed into muddy from limpid, and bacterial number increases, OD600Reach
To more than 2.5, compare with conventional medium, total number of bacteria is improved, time advance 7 days.Result shows, in conventional medium
Addition brown sugar, can significantly improve the growth and breeding speed of Thiobacillus thioxidans, shorten incubation time, realize Thiobacillus thioxidans
Quick flourish.
Claims (2)
- It is 1. a kind of to accelerate the culture medium prescription that Thiobacillus thioxidans breeds, it is characterised in that：Weight percentage,（NH4）2SO40.2%、 KH2PO40.3%、MgSO4·7H2O 0.05%、FeSO4·7H2O 0.001%、CaCl·2H2O 0.025%, sulphur Powder 1%, peptone 0.5%~1% and dusty yeast 0.5%~1%.
- 2. preferably, adding in a kind of culture medium prescription of the promotion Thiobacillus thioxidans fast breeding described in claim 1 With brown sugar, the amount for adding brown sugar is by weight percentage 5%~10%.
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| CN201611098103.1A CN106754490A (en) | 2016-12-03 | 2016-12-03 | A kind of culture medium for accelerating Thiobacillus thioxidans propagation |
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| CN201611098103.1A CN106754490A (en) | 2016-12-03 | 2016-12-03 | A kind of culture medium for accelerating Thiobacillus thioxidans propagation |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5366891A (en) * | 1992-03-18 | 1994-11-22 | Associated Universities, Inc. | Biochemical solubilization of toxic salts from residual geothermal brines and waste waters |
| CN101016521A (en) * | 2006-11-20 | 2007-08-15 | 山东大学 | Thermophilic thiobacillus gene engineering bacterium with mercury resistant characteristic and application thereof |
| CN103667131A (en) * | 2013-12-05 | 2014-03-26 | 中国科学院微生物研究所 | Method for increasing metal ore leaching rate, and special strain thereof |
-
2016
- 2016-12-03 CN CN201611098103.1A patent/CN106754490A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5366891A (en) * | 1992-03-18 | 1994-11-22 | Associated Universities, Inc. | Biochemical solubilization of toxic salts from residual geothermal brines and waste waters |
| CN101016521A (en) * | 2006-11-20 | 2007-08-15 | 山东大学 | Thermophilic thiobacillus gene engineering bacterium with mercury resistant characteristic and application thereof |
| CN103667131A (en) * | 2013-12-05 | 2014-03-26 | 中国科学院微生物研究所 | Method for increasing metal ore leaching rate, and special strain thereof |
Non-Patent Citations (4)
| Title |
|---|
| D.B. JOHNSON: "Selective solid media for isolating and enumerating acidophilic bacteria", 《JOURNAL OF MICROBIOLOGICAL METHODS》 * |
| 吕早生 等: "一株高浸磷嗜酸氧化硫硫杆菌的分离与鉴定", 《应用与环境生物学报》 * |
| 王磊: "α-酮戊二酸脱氢酶基因在嗜酸性氧化硫硫杆菌中的表达研究", 《中国优秀硕士学位论文全文数据库 基础科学辑》 * |
| 颜望明 等: "混合培养中几株真菌对氧化硫硫杆菌生长和硫的氧化的影响", 《山东大学学报（自然科学版）》 * |
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