CN103382057B - Method for treating cultivation wastewater - Google Patents
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- CN103382057B CN103382057B CN201310319210.2A CN201310319210A CN103382057B CN 103382057 B CN103382057 B CN 103382057B CN 201310319210 A CN201310319210 A CN 201310319210A CN 103382057 B CN103382057 B CN 103382057B
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Abstract
The invention belongs to the technical field of environmental protection and discloses a method for treating cultivation wastewater. According to the method, firstly, the cultivation wastewater is subjected to solid-liquid separation by a solid-liquid separator, separated solid is used for preparing fertilizers, liquid flows into a purification tank, then, a microbial preparation is added for purification treatment. The invention further discloses the microbial preparation for treating the cultivation wastewater. According to the method, a lot of fixed facilities adopted in the prior art are omitted, the method has the advantages of low investment and low operation costs, and the removing rate of ammonic nitrogen, nitro nitrogen and phosphorus in the wastewater can reach above 99%.
Description
Technical field
The present invention relates to a kind of method of processing waste water, particularly relate to a kind of method of processing breeding wastewater.
Background technology
Along with the fast development of our aquaculture, effective processing of breeding wastewater also seems especially important.Aquaculture waste water belongs to high concentrated organic wastewater, CODcr is up to more than 5000mg/l, ammonia nitrogen is up to more than 800mg/l, P is up to 50mg/l, direct discharging of waste water is entered to water body or storage place is improper, be subject to rain-out to enter water body, may cause the severe exacerbation of surface water or quality of groundwater.Because the leaching of livestock and poultry fecaluria is very strong, the leaching amounts such as nitrogen, phosphorus and water soluble organic substance in fecaluria are very large, if do not dealt carefully with, will enter Water table polluted underground water by rainwash and diafiltration.On the impact of surface water main manifestations be, large amount of organic matter enters after water body, organic decomposition, by the dissolved oxygen consuming in a large number in water, makes water body smelly; After the dissolved oxygen in water body significantly declines, large amount of organic matter can under anaerobic continue to decompose, and in decomposition, will produce the toxic gas such as methane, hydrogen sulfide, causes hydrobiont mortality; A large amount of suspended substances in waste water can make water body muddiness, the photosynthesis that reduces algae in water, limits hydrobiological normal activity, makes the hydrobiont of Organic pollutants sensitivity dead gradually, thereby further aggravate water bottom anoxic, water body assimilative capacity is reduced; Nitrogen, phosphorus can make body eutrophication, the result of eutrophication can make in water body nitrate and nitrite concentration too high, if it is poisoning that people and animals' long-term drinking can cause, and the growth of some poisonous algae and amount reproduction can discharge a large amount of toxin in water body, cause the mortality of hydrocoles, thereby seriously destroyed water ecology balance.
At present, cultivation blowdown problem is never well solved, conventional treatment process cannot reach desirable treatment effect, mostly have that treatment facility investment is too large, working cost is too high, and waste water generation is large, complicated component, the processing after stain substrate concentration defects such as still very high, the used dilution water yield is many, be far from general breeding enterprise can bear.Therefore, seek that facility investment is few, working cost is low and the breeding wastewater treatment process of highly-efficient treatment, become and solved the key point that aquaculture is polluted.
Summary of the invention
The object of this invention is to provide a kind of method of breeding wastewater and microbial preparation of processing breeding wastewater processed.
The present invention processes in the method for breeding wastewater and has mainly adopted microbial preparation, and said preparation is environment friendly and pollution-free, with low cost, reasonable compatibility between the each bacterial classification in microbial preparation, symbiosis is coordinated, antagonism not mutually, and its preparation method is easy, easy to implement the method, it is easy and simple to handle, is beneficial to suitability for industrialized production.
The present invention adopts following technical scheme to realize:
A kind of method of processing breeding wastewater, first by breeding wastewater process solid-liquid separator, carry out solid-liquid separation, the solid of separation is as preparing fertilizer, and liquid enters treating pond, then regulating pH is 7-8, add 20 grams of microbial preparations by every cubic metre of liquid at every turn, add every day 1 time, add continuously one week, finally leave standstill 5 days, liquid is discharged.The preferred LAKOS efficient solid-liquid separator of above-mentioned solid-liquid separator JPL (JPX); Mentioned microorganism preparation is made up of activeconstituents and carrier; Activeconstituents and carrier are stirred, mix according to the weight ratio of 1:1, stir, then put cryodrying at 4 ℃, dry rear water content is controlled at 8-10%, to obtain final product; Described carrier is mixed to get according to the mass ratio of 3:2:1 by turfy soil, sawdust and diatomite.
Above-mentioned activeconstituents is made up of the raw material bacterium of following weight parts:
10 parts of nitrococcus, 8 parts of thiobacillus denitrificans, 7 parts of Pseudomonas stutzeris, 6 parts of sphingosine sporangiums, have a liking for 6 parts of the ferrous thiobacilluss of acid oxidase, 5 parts of Bacillus licheniformis, 3 parts of Phanerochaete chrysosporiums, 2 parts of Arthrobacter globiformis, 2 parts of rhodococcus, 1 part of bacillus cereus; The concentration of above-mentioned bacterium is all controlled at 2 × 10
8individual/gram.
Preferably,
Described nitrococcus is nitrococcus (Nitrosomonas sp.) CCTCC No:M 2010002 (CN101955885 openly uses);
Described thiobacillus denitrificans is for example reference APPLIED AND ENVIRONMENTAL MICROBIOLOGY of thiobacillus denitrificans (Thiobacillus denitrificans) ATCC 25259(, May 2007, p. 3265 – 3271);
Described Pseudomonas stutzeri is that Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M 209107(is referring to CN101705202A);
Described sphingosine sporangium is the visible CN102168054A of sphingosine sporangium (Sphingomonas sp.) CGMCC NO.4589();
Describedly have a liking for the ferrous thiobacillus of acid oxidase for having a liking for the visible document A of acid oxidase ferrous iron thiobacillus (Acidithiobacillus ferrooxidans) ATCC 53993(genomic island provides Acidithiobacillus ferrooxidans ATCC 53993 additional copper resistance:a possible competitive advantage. Appl Microbiol Biotechnol. 2011);
Described Bacillus licheniformis is the visible CN101037659A of Bacillus licheniformis (Bacillus licheniformis) CCTCC NO.M206082()
Described Phanerochaete chrysosporium is for example APPLIED AND ENVIRONMENTAL MICROBIOLOGY of Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC 24725(, Feb1994, p709-714);
Described Arthrobacter globiformis is Arthrobacter globiformis (Arthrobacter globiformis) CCTCC NO:M209015(CN101492649A);
Described rhodococcus is that rhodococcus (Rhodococcus rhodochrous) ATCC 15906(is for example referring to Cloning and Characterization of Benzoate Catabolic Genes in the Gram-Positive Polychlorinated Biphenyl DegraderRhodococcus sp. Strain RHA1, J. Bacteriol.November 2001);
Described bacillus cereus is that wax-like gemma bar (Bacillus cereus) specifically can be for example New J. Chem. of ATCC 10876(, 2007,
31, 748-755).
Bacterium of the present invention all can be bought and obtain from commercial sources such as CGMCC, CCTCC and US mode culture collection warehousings (ATCC).
The enlarged culturing of each bacterial classification of the present invention is the cellar culture mode of this area, preferentially, has operated in the following manner:
The training method of nitrococcus is: nitrococcus is first on substratum, and 28-30 ℃, makes primary inclined plane and cultivate, and then secondary seed is cultivated, mixing fermentation culture to viable count in product reaches 2.0 × 10
8individual/gram, described medium component is: 2% glucose (w/v), 1.5% extractum carnis (w/v), 1.5% peptone (w/v), 0.058% magnesium sulfate (w/v), 0.025% manganous sulfate (w/v), 0.22% sodium acetate (w/v), 0.2% dipotassium hydrogen phosphate (w/v), solvent is water;
The training method of thiobacillus denitrificans is: be inoculated into (Na on substratum
2s
2o
35H
2o 5 g, KNO
32 g, KH
2pO
42 g, NaHCO
31 g, MgCl
26H2O 0.5 g, FeSO47H2O 0.01 g NH
4cl 0.5g, water 1000ml), 28-30 ℃, makes primary inclined plane and cultivates, and then secondary seed is cultivated, mixing fermentation culture to viable count in product reaches 2 × 10
8individual/gram;
The training method of having a liking for the ferrous thiobacillus of acid oxidase is: in 9K substratum ((NH
4) SO
43g/L, KCl 0.1g/L, K
2hPO
40.5g/L, Ca (NO
3)
20.01g/L, FeSO
47H
2o 44.43g/L) 28-30 ℃ is cultured to viable count in product and reaches 2.0 × 10
8individual/gram;
The training method of Bacillus licheniformis or bacillus cereus is: first Bacillus licheniformis or bacillus cereus test tube kind are seeded on beef-protein medium, 28-30 ℃, making primary inclined plane cultivates, then be inoculated into and in triangular flask, do vibration secondary liquid culture, then proceed to liquid fermentation tank and do three grades of liquid culture, finally be inoculated into and on solid medium, make level Four and cultivate, reach 2 × 10 to viable count in product
8individual/gram;
The training method of Pseudomonas stutzeri or sphingosine sporangium is: Pseudomonas stutzeri or sphingosine sporangium are seeded in first respectively on ox yeast extract paste protein culture medium substratum, 28-30 ℃, then does that level liquid seed culture, secondary liquid seeds are cultivated and fermentor cultivation to viable count in product reaches 2.0 × 10
8individual/gram;
The training method of Phanerochaete chrysosporium or Arthrobacter globiformis is: first Phanerochaete chrysosporium is inoculated on potato dextrose agar, 28-30 ℃, making primary inclined plane cultivates, then be inoculated into and in triangular flask, do vibration secondary liquid culture, then proceed to liquid fermentation tank and do three grades of liquid culture, finally be inoculated into and on solid medium, make level Four and cultivate, reach 2 × 10 to viable count in product
8individual/gram;
The training method of rhodococcus is: rhodococcus bacterial classification is seeded in to nutrient broth medium, and 28-30 ℃, makes primary inclined plane and cultivate, and then secondary seed is cultivated, mixing fermentation culture to viable count in product reaches 2.0 × 10
8individual/gram.
The invention also discloses a kind of microbial preparation of processing breeding wastewater.
The main beneficial effect that the present invention brings is as follows:
A large amount of fixation means that the present invention has avoided prior art to adopt, have the advantages that less investment and working cost are low;
The present invention processes in the method for breeding wastewater and has mainly adopted microbial preparation, and said preparation is environment friendly and pollution-free, with low cost, reasonable compatibility between the each bacterial classification in microbial preparation, symbiosis is coordinated, antagonism not mutually, and its preparation method is easy, easy to implement the method, it is easy and simple to handle, is beneficial to suitability for industrialized production;
Simple and the good purification of operating process of the present invention, possesses application prospect widely.
Embodiment
By the mode that adopts specific embodiment, the present invention is further elaborated below, but it should not be construed the restriction to core initiative spirit of the present invention.
Embodiment 1
A method of processing breeding wastewater, comprises the steps:
First by breeding wastewater process solid-liquid separator, carry out solid-liquid separation, the solid separating is as preparing fertilizer, liquid enters treating pond, and then regulating pH is 7-8, adds 20 grams of microbial preparations by every cubic metre of liquid at every turn, add every day 1 time, add continuously one week, finally leave standstill 5 days, liquid is discharged; Detect and find, the clearance of ammonia nitrogen, nitrate and the phosphorus of waste water can reach more than 99%.
Above-mentioned this microbial preparation is made up of activeconstituents and carrier; Preparation method is: activeconstituents is mixed according to the weight ratio of 1:1 with carrier, stir, then put cryodrying at 4 ℃, dry rear water content is controlled at 8-10%, to obtain final product; Described carrier is mixed to get according to the mass ratio of 3:2:1 by turfy soil, sawdust and diatomite.
Above-mentioned activeconstituents is made up of the raw material bacterium of following weight parts: 10 parts of nitrococcus, 8 parts of thiobacillus denitrificans, 7 parts of Pseudomonas stutzeris, 6 parts of sphingosine sporangiums, have a liking for 6 parts of the ferrous thiobacilluss of acid oxidase, 5 parts of Bacillus licheniformis, 3 parts of Phanerochaete chrysosporiums, 2 parts of Arthrobacter globiformis, 2 parts of rhodococcus, 1 part of bacillus cereus; The concentration of above-mentioned bacterium is all controlled at 2 × 10
8individual/gram;
Embodiment 2
A method of processing breeding wastewater, comprises the steps:
First by breeding wastewater process solid-liquid separator, carry out solid-liquid separation, the solid separating is as preparing fertilizer, liquid enters treating pond, and then regulating pH is 7-8, adds 20 grams of microbial preparations by every cubic metre of liquid at every turn, add every day 1 time, add continuously one week, finally leave standstill 5 days, liquid is discharged;
Above-mentioned this microbial preparation is made up of activeconstituents and carrier; Preparation method is: activeconstituents is mixed according to the weight ratio of 1:1 with carrier, stir, then put cryodrying at 4 ℃, dry rear water content is controlled at 8-10%, to obtain final product; Described carrier is mixed to get according to the mass ratio of 3:2:1 by turfy soil, sawdust and diatomite.
Above-mentioned activeconstituents is made up of the raw material bacterium of following weight parts: 10 parts of nitrococcus, 8 parts of thiobacillus denitrificans, 7 parts of Pseudomonas stutzeris, 6 parts of sphingosine sporangiums, have a liking for 6 parts of the ferrous thiobacilluss of acid oxidase, 5 parts of Bacillus licheniformis, 3 parts of Phanerochaete chrysosporiums, 2 parts of Arthrobacter globiformis, 2 parts of rhodococcus, 1 part of bacillus cereus; The concentration of above-mentioned bacterium is all controlled at 2 × 10
8individual/gram;
Described nitrococcus is nitrococcus (Nitrosomonas sp.) CCTCC No:M 2010002;
Described thiobacillus denitrificans is thiobacillus denitrificans (Thiobacillus denitrificans) ATCC 25259;
Described Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M 209107;
Described sphingosine sporangium is sphingosine sporangium (Sphingomonas sp.) CGMCC NO.4589;
Describedly have a liking for the ferrous thiobacillus of acid oxidase for having a liking for acid oxidase ferrous iron thiobacillus (Acidithiobacillus ferrooxidans) ATCC 53993;
Described Bacillus licheniformis is Bacillus licheniformis (Bacillus licheniformis) CCTCC NO.M206082;
Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC 24725;
Described Arthrobacter globiformis is Arthrobacter globiformis (Arthrobacter globiformis) CCTCC NO:M209015;
Described rhodococcus is rhodococcus (Rhodococcus rhodochrous) ATCC 15906;
Described bacillus cereus is that wax-like gemma bar (Bacillus cereus) is ATCC 10876.
Embodiment 3
Take Jiang Quan group breeding wastewater as example, detect the water treatment effect of embodiment 2: the detection index of breeding wastewater is in table 1:
Parameter index before and after table 1 wastewater treatment
| Project name | Water quality before treatment | Water quality after treatment | Desirable index |
| NH3-N(ammonia nitrogen) | 830mg/L | 0.2mg/L | ≤1mg/L |
| CODcr | 8400mg/L | 30mg/L | ≤40mg/L |
| N02-N(nitrate) | 120mg/L | 0.1mg/L | ≤1mg/L |
| P | 70mg/L | 0.03mg/L | ≤0.1mg/L |
Conclusion: by processing, in waste water, the content of every pollutent all reduces greatly, meets emission standard.
Claims (1)
1. a method of processing breeding wastewater, is characterized in that, described method comprises the steps:
First by breeding wastewater process solid-liquid separator, carry out solid-liquid separation, the solid separating is as preparing fertilizer, liquid enters treating pond, and then regulating pH is 7-8, adds 20 grams of microbial preparations by every cubic metre of liquid at every turn, add every day 1 time, add continuously one week, finally leave standstill 5 days, liquid is discharged;
Described microbial preparation is made up of activeconstituents and carrier; Described activeconstituents is made up of the raw material bacterium of following weight parts: 10 parts of nitrococcus, 8 parts of thiobacillus denitrificans, 7 parts of Pseudomonas stutzeris, 6 parts of sphingosine sporangiums, have a liking for 6 parts of the ferrous thiobacilluss of acid oxidase, 5 parts of Bacillus licheniformis, 3 parts of Phanerochaete chrysosporiums, 2 parts of Arthrobacter globiformis, 2 parts of rhodococcus, 1 part of bacillus cereus;
Described carrier is mixed to get according to the mass ratio of 3:2:1 by turfy soil, sawdust and diatomite;
The preparation method of described microbial preparation is: described activeconstituents is mixed according to the weight ratio of 1:1 with described carrier, stir, then put cryodrying at 4 ℃, dry rear water content is controlled at 8-10%, to obtain final product.
2. the method for claim 1, is characterized in that, described nitrococcus is nitrococcus (Nitrosomonas sp.) CCTCC No:M 2010002;
Described thiobacillus denitrificans is thiobacillus denitrificans (Thiobacillus denitrificans) ATCC 25259;
Described Pseudomonas stutzeri is Pseudomonas stutzeri (Pseudomonas stutzeri) CCTCC NO:M 209107;
Described sphingosine sporangium is sphingosine sporangium (Sphingomonas sp.) CGMCC NO.4589;
Describedly have a liking for the ferrous thiobacillus of acid oxidase for having a liking for acid oxidase ferrous iron thiobacillus (Acidithiobacillus ferrooxidans) ATCC 53993;
Described Bacillus licheniformis is Bacillus licheniformis (Bacillus licheniformis) CCTCC NO.M 206082;
Described Phanerochaete chrysosporium is Phanerochaete chrysosporium (Phanerochaete chrysosporium) ATCC 24725;
Described Arthrobacter globiformis is Arthrobacter globiformis (Arthrobacter globiformis) CCTCC NO:M 209015;
Described rhodococcus is rhodococcus (Rhodococcus rhodochrous) ATCC 15906;
Described bacillus cereus is that bacillus cereus (Bacillus cereus) is ATCC 10876.
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| CN110092542A (en) * | 2019-04-30 | 2019-08-06 | 江西省赣抚平原水利工程管理局(江西省灌溉试验中心站) | A kind of small rural sewage treatment system for farm and method |
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