CN106754450B - Denitrifying microorganism culture promoter and preparation method and application thereof - Google Patents
Denitrifying microorganism culture promoter and preparation method and application thereof Download PDFInfo
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- CN106754450B CN106754450B CN201510802225.3A CN201510802225A CN106754450B CN 106754450 B CN106754450 B CN 106754450B CN 201510802225 A CN201510802225 A CN 201510802225A CN 106754450 B CN106754450 B CN 106754450B
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Abstract
The invention discloses a denitrifying microorganism culture promoter which comprises metal salt, polyamine substances, organic acid hydroxylamine and organic acid salt, wherein the metal salt is composed of calcium salt, copper salt, magnesium salt and/or ferrous salt. The preparation method comprises (1) preparing metal salt solution according to certain composition and weight parts; (2) before use, polyamine substances, organic acid hydroxylamine and organic acid salt are added into metal salt solution. The denitrifying microorganism culture promoter disclosed by the invention is simple in formula and easy to prepare, the cultured denitrifying microorganism has high activity and strong impact resistance, and can perform denitrification reaction by taking nitrite nitrogen as an electron acceptor. The accelerator can be used in the culture process of denitrifying microorganisms taking nitrite nitrogen as an electron acceptor, and can also be directly added into the existing sewage treatment system to promote the smooth running of the short-cut nitrification and denitrification process.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a denitrifying microorganism culture promoter taking nitrite nitrogen as an electron acceptor, and a preparation method and application thereof.
Background
Denitrifying bacteria are a type of facultative anaerobic microorganisms that convert nitrate or nitrite nitrogen into nitrogen. The microorganisms can induce nitrate reductase A and nitrite reductase required by denitrification only under anaerobic condition, and nitrate or nitrite is taken as an electron acceptor for denitrification. Because of different environments required by microorganisms, the impact resistance of the system is poor in the whole denitrification process of sewage treatment, namely the nitrification process and the denitrification process, high-concentration ammonia nitrogen and nitrate nitrogen can inhibit the growth of nitrifying bacteria, and high-concentration dissolved oxygen and carbon source deficiency can inhibit the growth of denitrifying bacteria, so that the total denitrification effect is influenced. However, at present, there is no method for effectively obtaining denitrifying bacteria, so that the obtained denitrifying bacteria can be used for sewage treatment for nitrification and denitrification.
In recent years, loss of total nitrogen has been frequently found in many aerobic denitrification tanks in actual operation (experimental studies on simultaneous nitrification and denitrification, von willebrand disease, and the like, Shanghai environmental science, 2002, 21). Many studies and reports at home and abroad also confirm the existence of aerobic denitrification (Ding ai, moderate, experimental evidence of aerobic biological denitrification reaction [ J ] scientific report, 2000, 45). Therefore, the denitrification technology is developed by using the aerobic denitrifying bacteria, the denitrification process can be carried out in the same reactor, and the occupied area and the construction investment are greatly reduced; the dosage of chemical substances and carbon sources for adjusting the pH of the system can be reduced, the medicament cost is saved, and the running cost is reduced; COD (chemical oxygen demand) can be removed while denitrification is carried out, the shock resistance of the system is enhanced, and the treatment effect is good.
CN200610023388.2 discloses a method for denitrifying nitrite by using granular sludge, which has the advantages of high sludge concentration in a system, high reactor efficiency, simple process and the like, but the denitrifying granular sludge can be obtained only after being cultured for 30-90 days at 15-30 ℃, and the concentration of nitrite nitrogen in the treated wastewater is 10-200 mg/L. CN200910011759.9 discloses a method for culturing shortcut denitrification granular sludge, which is to realize enrichment culture of denitrification granular sludge in three stages by selecting different culture matrixes. CN201010536004.3 discloses a denitrifying bacterial agent for denitrification by nitrite and application thereof, wherein the denitrifying bacterial agent is mainly prepared by bacterial strains with special combination and proportion. If a substance suitable for the growth of the denitrifying microorganisms is directly added into the sewage treatment system, the enrichment culture process of the denitrifying microorganisms can be greatly simplified, and the culture cost of the denitrifying microorganisms can be further reduced.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a denitrifying microorganism culture promoter and a preparation method and application thereof. The accelerator has simple formula and easy preparation. The cultured denitrifying microorganisms have high activity and strong impact resistance, and can perform denitrification reaction by taking nitrite nitrogen as an electron acceptor; can be used in the culture process of denitrifying microorganisms taking nitrite nitrogen as an electron acceptor, and can also be directly added into a sewage treatment system to promote the smooth running of the short-cut nitrification and denitrification process.
The denitrifying microorganism culture promoter comprises metal salt, polyamine substances, organic acid hydroxylamine and organic acid salt, wherein the metal salt consists of calcium salt, copper salt, magnesium salt and/or ferrous salt.
In the culture promoter for denitrifying microorganisms, the metal salt is 40 to 100 parts by weight, preferably 50 to 80 parts by weight, the polyamine substance is 5 to 30 parts by weight, preferably 10 to 20 parts by weight, the organic acid hydroxylamine is 0.5 to 15 parts by weight, preferably 2 to 10 parts by weight, and the organic acid salt is 5 to 30 parts by weight, preferably 10 to 20 parts by weight.
In the denitrifying microorganism culture promoter of the present invention, when the metal salt is a calcium salt, a magnesium salt and a copper salt, Ca is contained therein2+、Mg2+And Cu2+The molar ratio of (5-15): (5-25): 0.5-5), preferably (8-12): 10-20): 1-4; or when the metal salt is a calcium salt, a ferrous salt and a copper salt, wherein Ca is2+、Fe2+And Cu2+The molar ratio of (5-15): (1-8): 0.5-5, preferably (8-12): 2-6): 1-4; or the metal salt is calcium salt, magnesium salt, ferrous salt and copper salt, wherein Ca2+、Mg2+、Fe2+And Cu2+The molar ratio of (5-15): (5-25): 1-8): 0.5-5, preferably (8-12): 10-20): 2-6): 1-4.
In the denitrifying microorganism culture promoter, the calcium salt is CaSO4Or CaCl2Preferably CaSO4(ii) a The magnesium salt is MgSO4Or MgCl2Preferably MgSO (MgSO)4(ii) a The ferrous salt is FeSO4Or FeCl2Preferably FeSO4(ii) a The copper salt being CuSO4Or CuCl2Preferably CuSO4。
In the denitrifying microorganism culture promoter, the polyamine substance is spermine, spermidine or a mixture of spermine and spermidine. The organic acid hydroxylamine is hydroxylamine formate, hydroxylamine acetate or a mixture of the two. The organic acid salt is one or more of sodium acetate, sodium succinate, sodium citrate and the like, is helpful for inducing nitrite reductase required by denitrification, and has good denitrification effect.
The preparation method of the denitrification microorganism culture promoter comprises the following steps: (1) preparing a metal salt solution according to the following components in parts by weight: the metal salt is 40-100 parts by weight, preferably 50-80 parts by weight, and the metal salt consists of calcium salt, copper salt, magnesium salt and/or ferrous salt; (2) before use, 5 to 30 weight portions, preferably 10 to 20 weight portions of polyamine substance, 0.5 to 15 weight portions, preferably 2 to 10 weight portions of organic acid hydroxylamine and 5 to 30 weight portions, preferably 10 to 20 weight portions of organic acid salt are added into the metal salt solution.
When the metal salt in step (1) of the present invention is a calcium salt, a magnesium salt or a copper salt, wherein Ca is2+、Mg2+And Cu2+The molar ratio of (5-15): (5-25): 0.5-5), preferably (8-12): 10-20): 1-4; or the metal salt is a calcium salt, a ferrous salt and a copper salt, wherein Ca is2+、Fe2+And Cu2+The molar ratio of (5-15): (1-8): 0.5-5, preferably (8-12): 2-6): 1-4; or the metal salt is calcium salt, magnesium salt, ferrous salt and copper salt, wherein Ca2+、Mg2+、Fe2+And Cu2+The molar ratio of (5-15): (5-25): 1-8): 0.5-5, preferably (8-12): 10-20): 2-6): 1-4.
The calcium salt in the step (1) of the invention is CaSO4Or CaCl2Preferably CaSO4(ii) a The magnesium salt is MgSO4Or MgCl2Preferably MgSO (MgSO)4(ii) a The ferrous salt is FeSO4Or FeCl2Preferably FeSO4(ii) a The copper salt being CuSO4Or CuCl2Preferably CuSO4。
The polyamine substance in the step (2) of the invention is spermine, spermidine or a mixture of the spermine and the spermidine. The organic acid hydroxylamine is hydroxylamine formate, hydroxylamine acetate or a mixture of the two. The organic acid salt is one or more of sodium acetate, sodium succinate, sodium citrate and other organic acid salts.
The application of the denitrifying microorganism growth promoter can be used in the culture process of denitrifying microorganisms, can also be used for improving the total nitrogen removal effect of the existing sewage treatment system, and can also be used for quickly starting the denitrification process of an ammonia-containing system and quickly recovering an impacted system. The dosage is determined according to the sewage property, the treatment capacity and the treatment effect. During the use process, the accelerant is firstly dissolved and then added according to the concentration of the accelerant in the sewage of 10-100mg/L, preferably 10-50 mg/L.
Compared with the prior art, the invention has the following beneficial effects:
(1) the metal ions provided by the metal salt are used as elements required by the growth of denitrifying microorganisms, and are used as enzyme components to improve the activity of the enzyme, so that substrates can be quickly degraded, and the enzymatic reaction process is accelerated. The polyamine substance and the metal ions act together to accelerate cell proliferation, improve the settleability and stability of the harvested thalli and prolong the service life of the thalli.
(2) The organic acid hydroxylamine and the organic acid salt can slowly release the organic acid, and the release of the low-concentration organic acid is beneficial to the growth of denitrifying microorganisms, the group effect of the microorganisms is favorably exerted, and the pollutant degrading capability of flora is improved.
(3) The invention realizes the purpose of rapidly culturing denitrifying microorganisms under the combined action of metal salt, polyamine substances, organic acid hydroxylamine and organic acid salt by selecting the components and the proportion of the denitrifying microorganism growth promoter. The denitrifying microorganism obtained by the invention still has higher denitrification effect under the condition of insufficient carbon source, and can well solve the problem of rapid recovery of denitrification capability of an impacted sewage treatment system.
Detailed Description
The present invention will be described in detail with reference to specific examples.
The formulation and ratio of the denitrifying microorganism growth promoter of examples 1-3 and comparative examples 1-4 are specifically shown in Table 1, and a metal salt solution is first prepared according to the formulation of Table 1; before use, polyamine substances, organic acid hydroxylamine and organic acid salt are added into metal salt solution.
TABLE 1 formulation and proportions of accelerators
The COD concentration of the wastewater generated by a certain enterprise is 500mg/L, the ammonia nitrogen concentration is 200mg/L, the wastewater is treated by adopting a short-cut nitrification and denitrification process, the average COD concentration of the treated effluent is 80mg/L, the ammonia nitrogen concentration of the treated effluent is lower than 15mg/L, the total nitrogen concentration of the effluent is up to 70mg/L, and the nitrogen concentration of nitrite is 50 mg/L. The growth promoter with the concentration of 0.5g/L is prepared by using the promoters prepared according to the proportion in the table 1, the growth promoter is added according to the concentration of the promoter in the sewage of 10mg/L every day, and after the growth promoter is added for 10 days, the total nitrogen concentration of effluent is lower than 50mg/L, and the nitrite nitrogen concentration is lower than 30 mg/L. The system is continuously operated for 10 days, the COD, ammonia nitrogen, total nitrogen and nitrite nitrogen concentration of the effluent are sampled and analyzed, and the treatment effect is shown in the table 2.
TABLE 2 treatment Effect with different formulations of accelerators
From the data in Table 2, the initial COD was further reduced to less than 60mg/L after the accelerator was used, the total nitrogen concentration in the effluent was less than 25mg/L, and the nitrite nitrogen concentration was less than 1.5 mg/L. When the accelerator lacks any one of the formulas, the total nitrogen concentration of effluent is higher than 30mg/L, and the nitrogen concentration of nitrite is higher than 15 mg/L. Therefore, the growth promoter can obviously improve the total nitrogen removal rate in the sewage.
Claims (5)
1. The culture promoter for denitrifying microorganisms is characterized by comprising 40-100 parts by weight of metal salt, 5-30 parts by weight of polyamine substances, 0.5-15 parts by weight of organic acid hydroxylamine and organic acid salt5-30 parts by weight; the polyamine substance is spermine or spermidine; the organic acid hydroxylamine is hydroxylamine formate or hydroxylamine acetate; the organic acid salt is one or more of sodium acetate, sodium succinate and sodium citrate; the metal salt is calcium salt, magnesium salt and copper salt, wherein Ca2+、Mg2+And Cu2+The molar ratio of (5-15) to (5-25) to (0.5-5); or the metal salt is a calcium salt, a ferrous salt and a copper salt, wherein Ca is2+、Fe2+And Cu2+The molar ratio of (5-15) to (1-8) to (0.5-5); or the metal salt is calcium salt, magnesium salt, ferrous salt and copper salt, wherein Ca2+、Mg2+、Fe2+And Cu2+The molar ratio of (5-15): (5-25): 1-8): 0.5-5.
2. The accelerator according to claim 1, characterized in that: the calcium salt is CaSO4Or CaCl2Magnesium salt is MgSO4Or MgCl2The ferrous salt is FeSO4Or FeCl2The copper salt is CuSO4Or CuCl2。
3. A method for preparing a culture promoter for denitrifying microorganisms is characterized by comprising the following steps: (1) preparing a metal salt solution according to the following components in parts by weight: the metal salt is 40-100 weight parts, and the metal salt is calcium salt, magnesium salt and copper salt, wherein Ca2+、Mg2+And Cu2+The molar ratio of (5-15) to (5-25) to (0.5-5); or the metal salt is a calcium salt, a ferrous salt and a copper salt, wherein Ca is2+、Fe2+And Cu2+The molar ratio of (5-15) to (1-8) to (0.5-5); or the metal salt is calcium salt, magnesium salt, ferrous salt and copper salt, wherein Ca2+、Mg2+、Fe2+And Cu2+The molar ratio of (5-15): (5-25): (1-8): 0.5-5); (2) before use, adding 5-30 parts by weight of polyamine substance, 0.5-15 parts by weight of organic acid hydroxylamine and 5-30 parts by weight of organic acid salt into metal salt solution; the polyamine substance is spermine or spermidineSpermine; the organic acid hydroxylamine is hydroxylamine formate or hydroxylamine acetate; the organic acid salt is one or more of sodium acetate, sodium succinate and sodium citrate.
4. The method of claim 3, wherein: the calcium salt is CaSO4Or CaCl2Magnesium salt is MgSO4Or MgCl2The ferrous salt is FeSO4Or FeCl2The copper salt is CuSO4Or CuCl2。
5. Use of the culture promoter for denitrifying microorganisms according to any one of claims 1 to 2, wherein: the accelerant is used in the culture process of denitrifying microorganisms, or used for improving the total nitrogen removal effect of the existing sewage treatment system, or used for quickly starting the denitrification process of an ammonia-containing system and quickly recovering an impacted system; during the use process, firstly, the accelerant is dissolved, and then the accelerant is added according to the concentration of 10-100mg/L in the sewage.
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| CN108118022B (en) | 2016-11-29 | 2020-09-11 | 中国石油化工股份有限公司 | Microbial culture promoter for completing denitrification process and application thereof |
| CN111099742A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Composition for improving resistance of denitrification system and denitrification method thereof |
| CN111099743B (en) * | 2018-10-26 | 2022-06-07 | 中国石油化工股份有限公司 | Composition for enhancing denitrification and denitrification of wastewater and denitrification method thereof |
| CN110040846B (en) * | 2019-05-06 | 2021-10-22 | 普罗生物技术（上海）有限公司 | Denitrification accelerant and application thereof |
| CN110902811A (en) * | 2019-12-10 | 2020-03-24 | 西安益维普泰环保股份有限公司 | Sewage composite carbon source agent and preparation method thereof |
| CN111362421A (en) * | 2019-12-30 | 2020-07-03 | 上海波赛统环境科技有限公司 | Medicament and method for removing nitrogen and phosphorus from eutrophic water body by microorganisms |
| CN114873739B (en) * | 2022-05-17 | 2023-12-08 | 山东圣奥生物科技有限公司 | Nitrite reductase promoter and application thereof |
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