CN106754450A - A kind of denitrifying microorganism culture promoter and its preparation method and application - Google Patents
A kind of denitrifying microorganism culture promoter and its preparation method and application Download PDFInfo
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- CN106754450A CN106754450A CN201510802225.3A CN201510802225A CN106754450A CN 106754450 A CN106754450 A CN 106754450A CN 201510802225 A CN201510802225 A CN 201510802225A CN 106754450 A CN106754450 A CN 106754450A
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Abstract
The invention discloses a kind of denitrifying microorganism culture promoter, including slaine, polyamines, organic acid azanol and acylate, the slaine is made up of calcium salt, mantoquita, magnesium salts and/or ferrous salt.Its preparation method includes（1）Metal salt solution is prepared according to definite composition and weight portion；（2）Using preceding by polyamines, organic acid azanol and acylate are added in metal salt solution.Denitrifying microorganism culture promoter formula of the present invention is simple, and it is easy to prepare, and the denitrifying microorganism activity cultivated is high, tolerance impact capacity is strong, can carry out denitrification reaction as electron acceptor using nitrite nitrogen.During the accelerator can be used for using nitrite nitrogen as the incubation of the denitrifying microorganism of electron acceptor, it is also possible to be directly added in existing sewage disposal system, promote being smoothed out for short-cut nitrification and denitrification process.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of denitrifying microorganism culture promoter using nitrite nitrogen as electron acceptor and its preparation method and application.
Background technology
Denitrifying bacterium is the amphimicrobe that a class is converted into nitrate nitrogen or nitrite nitrogen nitrogen.This quasi-microorganism is general only under conditions of anaerobism, nitrate reductase A and nitrite reductase needed for just inducing denitrification, and denitrogenation is carried out by electron acceptor of nitrate or nitrite.The difference of environment is needed due to Institute of Micro-biology, therefore in the whole denitrification process of sewage disposal, that is nitrifying process and denitrification process, the impact resistance of system is poor, the ammonia nitrogen of high concentration, nitrate nitrogen can suppress the growth of nitrifier, dissolved oxygen and the carbon source deficiency of high concentration can suppress the growth of denitrifying bacterium, so as to influence denitrogenation general effect.But the method currently without denitrifying bacterium is effectively obtained, the denitrifying bacterium that will be obtained is nitrified and denitrification process denitrogenation for sewage disposal.
In recent years, in the aerobic denitrification pond in many actual motions, it is often found that there is the loss of total nitrogen（Feng Yecheng etc., the experimental study of simultaneous nitrification-denitrification, Shanghai environmental science, 2002,21）.Many researchs and report also demonstrate that the presence of aerobic denitrification both at home and abroad（Fourth likes medium, experimental evidence [J] Science Bulletin of aerobe anti-nitration reaction, 2000,45）.Therefore denitrogenation technology is developed using aerobic denitrifying bacteria, denitrification process can be made to be carried out in same reactor, greatly reduce floor space and construction investment；The chemical substance of regulating system pH and the consumption of carbon source can be reduced, medicament expense is saved and is used, reduce operating cost；COD can also be removed while denitrogenation（COD）, strengthening system impact resistance, high treating effect.
CN200610023388.2 discloses a kind of method that nitrite denitrification is carried out using granule sludge, although having the advantages that sludge concentration is high, reactor efficiency is high, process is simple in system, but denitrification granular sludge needs to be obtained after being cultivated 30-90 days under the conditions of 15-30 DEG C, and the cultured water concentration in handled waste water is 10-200mg/L.CN200910011759.9 discloses a kind of cultural method of granular sludge by shortcut denitrification, is the enrichment culture by selecting different culture matrixes point three phases to realize denitrification granular sludge.CN201010536004.3 disclose it is a kind of carry out denitrifying denitrogenation microbial inoculum and its application using nitrite, the invention is mainly and realizes denitrification denitrogenation using specific combination and the bacterial strain for matching.If the enrichment culture process of denitrifying microorganism directly can be greatly simplified to a kind of material for being suitable to denitrifying microorganism growth is added in sewage disposal system, it is possible to further reduce the toxigenic capacity of denitrifying microorganism.
The content of the invention
In view of the shortcomings of the prior art, the invention provides a kind of denitrifying microorganism culture promoter and its preparation method and application.The promotion agent prescription is simple, and it is easy to prepare.Denitrifying microorganism activity height, the tolerance impact capacity cultivated by force, can carry out denitrification reaction using nitrite nitrogen as electron acceptor；In can be used for using nitrite nitrogen as the incubation of the denitrifying microorganism of electron acceptor, it is also possible to be directly added in sewage disposal system, promote being smoothed out for short-cut nitrification and denitrification process.
Denitrifying microorganism culture promoter of the present invention, including slaine, polyamines, organic acid azanol and acylate, the slaine are made up of calcium salt, mantoquita, magnesium salts and/or ferrous salt.
In denitrifying microorganism culture promoter of the present invention, slaine is 40-100 weight portions, preferably 50-80 weight portions, polyamines are 5-30 weight portions, preferably 10-20 weight portions, organic acid azanol is 0.5-15 weight portions, preferably 2-10 weight portions, acylate is 5-30 weight portions, preferably 10-20 weight portions.
In denitrifying microorganism culture promoter of the present invention, when the slaine is calcium salt, magnesium salts and mantoquita, wherein Ca2+、Mg2+And Cu2+Mol ratio be（5-15）:（5-25）:（0.5-5）, preferably（8-12）:（10-20）:（1-4）；Or the slaine is when being calcium salt, ferrous salt and mantoquita, wherein Ca2+、Fe2+And Cu2+Mol ratio be（5-15）:（1-8）:（0.5-5）, preferably（8-12）:（2-6）:（1-4）；Or the slaine is calcium salt, magnesium salts, ferrous salt and mantoquita, wherein Ca2+、Mg2+、Fe2+And Cu2+Mol ratio be（5-15）:（5-25）:（1-8）:（0.5-5）, preferably（8-12）:（10-20）:（2-6）:（1-4）.
In denitrifying microorganism culture promoter of the present invention, described calcium salt is CaSO4Or CaCl2, preferably CaSO4；Magnesium salts is MgSO4Or MgCl2, preferably MgSO4；Ferrous salt is FeSO4Or FeCl2, preferably FeSO4；Mantoquita is CuSO4Or CuCl2, preferably CuSO4。
In denitrifying microorganism culture promoter of the present invention, described polyamines are the mixture of spermine, spermidine or both.Described organic acid azanol is the mixture of formic acid azanol, hydroxylamine acetate or both.Described acylate is one or more in the acylates such as sodium acetate, sodium succinate and sodium citrate, and acylate contributes to the nitrite reductase needed for inducing denitrification, and denitrification denitrogenation effect is good.
The preparation method of denitrifying microorganism culture promoter of the present invention, comprises the following steps：（1）Metal salt solution is prepared according to consisting of and weight portion：Slaine is 40-100 weight portions, preferably 50-80 weight portions, and the slaine is made up of calcium salt, mantoquita, magnesium salts and/or ferrous salt；（2）Using preceding by 5-30 weight portions, the preferably polyamines of 10-20 weight portions, the organic acid azanol and 5-30 weight portions of 0.5-15 weight portions, preferably 2-10 weight portions, the acylate of preferably 10-20 weight portions are added in metal salt solution.
Step of the present invention（1）When described slaine is calcium salt, magnesium salts and mantoquita, wherein Ca2+、Mg2+And Cu2+Mol ratio be（5-15）:（5-25）:（0.5-5）, preferably（8-12）:（10-20）:（1-4）；Or the slaine is calcium salt, ferrous salt and mantoquita, wherein Ca2+、Fe2+And Cu2+Mol ratio be（5-15）:（1-8）:（0.5-5）, preferably（8-12）:（2-6）:（1-4）；Or the slaine is calcium salt, magnesium salts, ferrous salt and mantoquita, wherein Ca2+、Mg2+、Fe2+And Cu2+Mol ratio be（5-15）:（5-25）:（1-8）:（0.5-5）, preferably（8-12）:（10-20）:（2-6）:（1-4）.
Step of the present invention（1）Described calcium salt is CaSO4Or CaCl2, preferably CaSO4；Magnesium salts is MgSO4Or MgCl2, preferably MgSO4；Ferrous salt is FeSO4Or FeCl2, preferably FeSO4；Mantoquita is CuSO4Or CuCl2, preferably CuSO4。
Step of the present invention（2）The polyamines are the mixture of spermine, spermidine or both.The organic acid azanol is the mixture of formic acid azanol, hydroxylamine acetate or both.Described acylate is one or more in the acylates such as sodium acetate, sodium succinate and sodium citrate.
The application of denitrifying microorganism growth promoter of the present invention, the accelerator can be used for the incubation of denitrifying microorganism, can be used for improving the total nitrogen removal effect of existing sewage disposal system, can be also used for the fast quick-recovery of quick startup and the system that is hit containing ammonia system denitrification process.It is specific to need to determine dosage according to sewage property, treating capacity and treatment effect.In use, accelerator is dissolved first, then according to promoter concentration 10-100mg/L in sewage, preferably 10-50 mg/L are added.
Compared with prior art, the invention has the advantages that：
（1）Element required for the metal ion as denitrifying microorganism growth provided by the use of slaine, while improving the activity of enzyme as the component of enzyme, can accelerate enzymatic reaction process with fast degradation substrate.Polyamines and metal ion collective effect, can accelerate cell and breed, and can improve the settleability and stability of harvested thalline, extend thalline service life.
（2）Organic acid azanol and acylate can slowly release organic acid, and the release of low-concentration organic acid is conducive to the growth of denitrifying microorganism, be conducive to playing the population effect of microorganism, improve the ability of microflora degradation pollutant.
（3）Component and proportioning by selecting denitrifying microorganism growth promoter of the invention, under the collective effect of slaine, polyamines, organic acid azanol and acylate, realizes the purpose of rapidly culturing denitrifying microorganism.The denitrifying microorganism that is obtained of the present invention can still have denitrification effect higher in the case of carbon source deficiency, moreover it is possible to solve the fast recovery problem of sewage disposal system denitrification ability being hit well.
Specific embodiment
The present invention program is described in detail with reference to specific embodiment.
The formula and ratio of the denitrifying microorganism growth promoter of embodiment 1-3 and comparative example 1-4 are specifically shown in Table 1, and the formula for being first according to table 1 prepares metal salt solution；Using preceding by polyamines, organic acid azanol and acylate are added in metal salt solution.
The formula and ratio of the accelerator of table 1
Waste water COD concentration produced by certain enterprise is 500mg/L, ammonia nitrogen concentration is 200mg/L, processed using short-cut nitrification and denitrification technique, effluent COD concentration average out to 80mg/L, water outlet ammonia nitrogen concentration are less than 15mg/L after treatment, water outlet total nitrogen concentration is up to 70mg/L, and its nitrogen concentration of nitrite is 50mg/L.The accelerator configured using the proportioning of table 1, configuration concentration is the growth promoter of 0.5g/L, and daily according to promoter concentration in sewage for 10mg/L is added, after adding 10 days, water outlet total nitrogen concentration is less than 50mg/L, and nitrite nitrogen concentration is less than 30mg/L.System is continued to run with 10 days, and sampling analysis water outlet COD, ammonia nitrogen, total nitrogen, nitrite nitrogen concentration, treatment effect are as shown in table 2.
Table 2 uses the treatment effect of different formulations accelerator
From the point of view of the data of table 2, it is further reduced to less than 60mg/L using initial COD after accelerator, water outlet total nitrogen concentration is less than 1.5mg/L less than 25mg/L, nitrite nitrogen concentration.When accelerator lacks any one of formula, water outlet total nitrogen concentration is above 30mg/L, and nitrite nitrogen concentration is above 15mg/L.As can be seen here, growth promoter of the invention can significantly improve nitrogen removal rate in sewage.
Claims (12)
1. a kind of denitrifying microorganism culture promoter, it is characterised in that including slaine, polyamines, organic acid azanol and acylate, the slaine is made up of calcium salt, mantoquita, magnesium salts and/or ferrous salt.
2. accelerator according to claim 1, it is characterised in that：Described slaine is 40-100 weight portions, and polyamines are 5-30 weight portions, and organic acid azanol is 0.5-15 weight portions, and acylate is 5-30 weight portions.
3. accelerator according to claim 1 and 2, it is characterised in that：When the slaine is calcium salt, magnesium salts and mantoquita, wherein Ca2+、Mg2+And Cu2+Mol ratio be（5-15）:（5-25）:（0.5-5）；Or the slaine is when being calcium salt, ferrous salt and mantoquita, wherein Ca2+、Fe2+And Cu2+Mol ratio be（5-15）:（1-8）:（0.5-5）；Or the slaine is calcium salt, magnesium salts, ferrous salt and mantoquita, wherein Ca2+、Mg2+、Fe2+And Cu2+Mol ratio be（5-15）:（5-25）:（1-8）:（0.5-5）.
4. accelerator according to claim 1, it is characterised in that：Described calcium salt is CaSO4Or CaCl2, magnesium salts is MgSO4Or MgCl2, ferrous salt is FeSO4Or FeCl2, mantoquita is CuSO4Or CuCl2。
5. accelerator according to claim 1 and 2, it is characterised in that：Described polyamines are the mixture of spermine, spermidine or both；Described organic acid azanol is the mixture of formic acid azanol, hydroxylamine acetate or both.
6. accelerator according to claim 1 and 2, it is characterised in that：Described acylate is one or more in the acylates such as sodium acetate, sodium succinate and sodium citrate.
7. a kind of preparation method of denitrifying microorganism culture promoter, it is characterised in that comprise the following steps：（1）Metal salt solution is prepared according to consisting of and weight portion：Slaine is 40-100 weight portions, and the slaine is made up of calcium salt, mantoquita, magnesium salts and/or ferrous salt；（2）Using the preceding polyamines by 5-30 weight portions, the organic acid azanol of 0.5-15 weight portions and the acylate of 5-30 weight portions are added in metal salt solution.
8. method according to claim 7, it is characterised in that：When described slaine is calcium salt, magnesium salts and mantoquita, wherein Ca2+、Mg2+And Cu2+Mol ratio be（5-15）:（5-25）:（0.5-5）；Or the slaine is calcium salt, ferrous salt and mantoquita, wherein Ca2+、Fe2+And Cu2+Mol ratio be（5-15）:（1-8）:（0.5-5）；Or the slaine is calcium salt, magnesium salts, ferrous salt and mantoquita, wherein Ca2+、Mg2+、Fe2+And Cu2+Mol ratio be（5-15）:（5-25）:（1-8）:（0.5-5）.
9. the method according to claim 7 or 8, it is characterised in that：Described calcium salt is CaSO4Or CaCl2, magnesium salts is MgSO4Or MgCl2, ferrous salt is FeSO4Or FeCl2, mantoquita is CuSO4Or CuCl2。
10. method according to claim 7, it is characterised in that：The polyamines are the mixture of spermine, spermidine or both；Organic acid azanol is the mixture of formic acid azanol, hydroxylamine acetate or both；Acylate is one or more in the acylates such as sodium acetate, sodium succinate and sodium citrate.
The application of any denitrifying microorganism growth promoters of 11. claim 1-6, it is characterised in that：The accelerator is used for the incubation of denitrifying microorganism, or for improving the total nitrogen removal effect of existing sewage disposal system, or for the quick startup containing ammonia system denitrification process and the fast quick-recovery of the system that is hit.
Application described in 12. claims 11, it is characterised in that：In use, accelerator is dissolved first, is then added according to promoter concentration 10-100mg/L in sewage.
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Cited By (7)
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| CN110040846A (en) * | 2019-05-06 | 2019-07-23 | 普罗生物技术（上海）有限公司 | A kind of denitrification promotor and application thereof |
| CN110902811A (en) * | 2019-12-10 | 2020-03-24 | 西安益维普泰环保股份有限公司 | Sewage composite carbon source agent and preparation method thereof |
| CN111099743A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Composition for enhancing denitrification and denitrification of wastewater and denitrification method thereof |
| CN111099742A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Composition for improving resistance of denitrification system and denitrification method thereof |
| CN111362421A (en) * | 2019-12-30 | 2020-07-03 | 上海波赛统环境科技有限公司 | Medicament and method for removing nitrogen and phosphorus from eutrophic water body by microorganisms |
| US11034932B2 (en) | 2016-11-29 | 2021-06-15 | China Petroleum & Chemical Corporation | Composition capable of promoting the growth of denitrifying microorganisms and uses thereof |
| CN114873739A (en) * | 2022-05-17 | 2022-08-09 | 山东圣奥生物科技有限公司 | Nitrite reductase promoter and application thereof |
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| US11034932B2 (en) | 2016-11-29 | 2021-06-15 | China Petroleum & Chemical Corporation | Composition capable of promoting the growth of denitrifying microorganisms and uses thereof |
| CN111099743A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Composition for enhancing denitrification and denitrification of wastewater and denitrification method thereof |
| CN111099742A (en) * | 2018-10-26 | 2020-05-05 | 中国石油化工股份有限公司 | Composition for improving resistance of denitrification system and denitrification method thereof |
| CN110040846A (en) * | 2019-05-06 | 2019-07-23 | 普罗生物技术（上海）有限公司 | A kind of denitrification promotor and application thereof |
| CN110902811A (en) * | 2019-12-10 | 2020-03-24 | 西安益维普泰环保股份有限公司 | Sewage composite carbon source agent and preparation method thereof |
| CN111362421A (en) * | 2019-12-30 | 2020-07-03 | 上海波赛统环境科技有限公司 | Medicament and method for removing nitrogen and phosphorus from eutrophic water body by microorganisms |
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