CN106745816A - The method of sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage - Google Patents
The method of sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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Abstract
The invention belongs to sanitary sewage disposal field, and in particular to the method for sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage.Technical scheme is as follows：Sial system ceramic porous material and high-efficient denitrification and dephosphorization bacterial strain are added in sanitary sewage, are processed；The dosage of the sial system ceramic porous material is 1~5g, and the high-efficient denitrification and dephosphorization bacterial strain access amount is one 1~10mL of bacteria suspension that OD600 values are 0.1~2；The bacteria suspension one is after high-efficient denitrification and dephosphorization bacterial strain activates 24h, to be obtained in being centrifuged on centrifuge, 8000~10000r/min of centrifuge speed, 10~12min of centrifugation time；The high-efficient denitrification and dephosphorization bacterial strain activation condition is：28~30 DEG C of temperature, pH 7~8,130~160r/min of shaking speed.Sial system ceramic porous material can increase the bond area of microorganism in the present invention, be improved through the microorganism concn of immobilization, be conducive to improving the removal efficiency of microbe, strengthen treatment effect.
Description
Technical field
The invention belongs to sanitary sewage disposal field, and in particular to a kind of sial system ceramic porous material immobilized microorganism
The method for processing sanitary sewage.
Background technology
As China's industry, the construction of agriculture, urbanization, sanitary sewage discharge capacity are increasing, caused environment is asked
Topic is also increasingly severe, and the research and development of the sewage disposal technology of high-efficiency and economic are subject to the extensive concern of numerous scholars.In recent years, for plus
Strong environmental pollution improvement, immobilized microorganism technology has obtained extensive research, at home and abroad in field of waste water treatment, microorganism
Immobilization technology has turned into a study hotspot.
Immobilized microorganism technology (Microorganisms immobilization technology) is according to fixation
Change zymotechnic development evolvement and come.Thalline settles more difficulty in free system, easily causes thalline and is lost in or solid-liquid
The problems such as hardly possible is separated, if using centrifuge or chemical coagulant, improve processing cost, increases popularization and application difficulty.
Under this situation, immobilized microorganism technology obtains exploitation with research.
Immobilized microorganism technology is mainly and for free microorganism (mainly bacterium and fungi) to be limited to certain space
In region, with means be mainly method physically or chemically, in the region, keep the activity of microorganism, be recycled profit
With.Conventional fixation support material is divided into 4 kinds：Natural carrier, artificial synthesized inorganic carrier, artificial synthesized organic polymer gather
Compound carrier and compound fixation support.Wherein natural carrier includes the grains of sand, zeolite, diatomite, porous resin, alginate
Deng；Artificial synthesized organic high molecular polymer carrier includes polyvinyl alcohol, polyurethane, polyethylene glycol, ethene, glutaraldehyde, dual
Nitrogen benzidine etc.；Artificial synthesized inorganic carrier is including porous ceramics, activated carbon, micropore glass, foam metal etc.；Complex carrier is just
It is to be mixed any of the above carrier material, prepares the compound carrier material body with good characteristic, to improve the performance of carrier,
Reduces cost, treatment effect of increasing water quality.All kinds of carriers respectively have advantage and disadvantage.Natural organic high-molecular carrier has stronger mass transfer
Property, but mechanical strength is relatively low, and the life-span is shorter；Artificial synthesized organic high molecular polymer mechanical strength is generally relatively strong, but mass transfer
Poor-performing, the activity of immobilized microorganism suffers from certain influence.Inorganic carrier has mechanical strength big, long lifespan, resistance to
The advantages of soda acid, but mass-transfer performance is undesirable.
The characteristics of immobilized microorganism technology treatment sewage has its unique, keeps the high concentration and height of microorganism living
Property, improve clearance, enhanced sewage treatment.Sludge quantity is few, and sedimentation is easily separated, and stability is strong, and poison resistance is high.Microorganism is through solid
Denitrification ability is ideal after fixedization, and current immobilized microorganism technology is widely used in nitrogenous sanitary sewage, low ratio of carbon to ammonium life
The treatment of the sewage such as sewage living, nitrogenous livestock breeding wastewater, dyeing waste water, and achieve good effect.But, at present should
Technology also has some problem demanding prompt solutions, such as：(1) more durable cheap immobilization novel carriers are researched and developed, with
Extension carrier service life, improves carrier mechanical performance, reduces cost expense；(2) more low toxicities or nontoxic are researched and developed
Fixation support, be allowed to minimum to the murder by poisoning of cell, to give full play to the function of cell degradation waste.
The content of the invention
The present invention provides a kind of method of sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage, sial system
Ceramic porous material can increase the bond area of microorganism, be improved through the microorganism concn of immobilization, it is ensured that microorganism
High concentration and high activity, be conducive to improve microbe removal efficiency, strengthen treatment effect.
Technical scheme is as follows：
A kind of method of sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage, sial system ceramic is porous
Material and high-efficient denitrification and dephosphorization bacterial strain are added in sanitary sewage, are processed；The sial system ceramic porous material is added
It is 1~5g to measure, and the high-efficient denitrification and dephosphorization bacterial strain access amount is one 1~10mL of bacteria suspension that OD600 values are 0.1~2；The bacterium
Suspension one is after high-efficient denitrification and dephosphorization bacterial strain activates 24h, to be obtained in being centrifuged on centrifuge, 8000~10000r/ of centrifuge speed
Min, 10~12min of centrifugation time；The high-efficient denitrification and dephosphorization bacterial strain activation condition is：28~30 DEG C of temperature, pH 7~8 shakes
130~160r/min of bed rotating speed.
The method of described sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage, wherein the sial system
Its mineral phase composition of ceramic porous material includes quartz, mullite and rutile；Its chemical composition is SiO2、Al2O3、Fe2O3、
K2O and impurity, SiO2Account for 56.78wt%, Al2O3Account for 37.11wt%, K2O accounts for 2.78wt%, Fe2O31.58wt% is accounted for, impurity is accounted for
1.75wt%；Its duct size is 30~1000 μm, and porosity is 30~90%, and particle diameter is 5-10mm；The sial system is porous
Material is synthesized by two kinds of natural clay CN-01 and CN-02；Quartz content accounts for 15.4% in the CN-01, and potassium feldspar accounts for 1.3%,
Muscovite accounts for 28.1%, and kaolinite accounts for 55.2%；Quartz content accounts for 10.4% in the CN-02, and potassium feldspar accounts for 0.6%, white clouds
Mother accounts for 16.9%, and kaolinite accounts for 72.1%；The CN-01 is with CN-02 grain diameters below 200 mesh；SiO in the CN-012
Account for 56.18wt%, Al2O326.80wt% is accounted for, bright alkali accounts for 8.70wt%, K2O accounts for 3.39wt%, Fe2O3Account for 2.63wt%, impurity
Account for 0.3wt%；SiO in the CN-022Account for 52.42wt%, Al2O331.98wt% is accounted for, bright alkali accounts for 10.90wt%, Fe2O3Account for
1.36wt%, K2O accounts for 2.04wt%, and impurity accounts for 1.3wt%.
The method of described sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage, its preferred scheme is,
The sial system ceramic porous material particle diameter is 2mm~2.5mm, is obtained using the method manually beaten.
The method of described sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage, wherein described efficiently de-
Nitrogen dephosphorization bacterial strain includes trivalent arsenic oxidizing bacteria (Acidovorax sp.), Burkholderia group
It is (Burkholderiacepacia group), spindle lysine bacillus (Lysinibacillusfusiformis), short
Microspore bacillus group (Bacilluspumilus group) and/or pseudomonas (Pseudomonas sp.).
The method of described sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage, the efficient denitrification is removed
The preparation process of phosphorus bacterial strain is as follows：
Step 1：The BC soil of operational excellence and the sanitary sewage disposal subsurface wastewater infiltration system of stabilization is taken, it is dilute with sterilized water
Release, vibration dissolving stands, and obtains bacteria suspension two；
Step 2：Strain isolation is carried out using dilution plate rubbing method, bacteria suspension two is coated into ordinary solid culture medium
On, strain growth situation is observed, plate streaking purifying is carried out to single bacterium colony, to obtain many plants of different strains；The ordinary solid
The chemical composition and concentration of culture medium be：Beef extract 5.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, agar 15.0~
20.0g/L, distilled water 1L/L；
Step 3：The many plants of different strains obtained with step 2 put into the simulation of human configuration with 1%~10% access amount
In sanitary sewage, simulated domestic wastewater is processed, investigate many plants of different strains to pollutant COD, NH4 +The removal of-N and TP
Effect, through screening after, classified using 16S rRNA identification systems, obtain the high-efficient denitrification and dephosphorization bacterial strain；The simulation
The chemical composition and concentration of sanitary sewage be：COD：200~400mg/L, NH4 +-N：20~60mg/L, TP：10~30mg/L.
Beneficial effects of the present invention are：
1st, sial system ceramic porous material can increase the bond area of microorganism, be carried through the microorganism concn of immobilization
It is high, it is ensured that the high concentration and high activity of microorganism.The aperture structure of sial system ceramic porous material is conducive to improving its mass transfer
Performance, is conducive to oxygen, nutriment etc. to enter material internal and decomposes utilization for microorganism, while being also beneficial to microorganism generation
Metabolite discharge material outer.Bacterial strain after being fixed using sial system ceramic porous material processes sanitary sewage, increases
The concentration of microorganism, is conducive to improving the removal efficiency of microbe, strengthens sewage treating efficiency.
2nd, the inventive method is easy to operate, and operating cost is low, and treatment effeciency is high, nonhazardous effect, good stability.
3rd, bacterial strain of the present invention is the sanitary sewage disposal subsurface wastewater infiltration system from operational excellence and stabilization, with efficient
Denitrogenation dephosphorizing function.
Brief description of the drawings
Fig. 1 a are sial system ceramic porous material structural scan electron microscope；
Fig. 1 b are bacterial strain PM3 form scanning electron microscope (SEM) photographs；
Fig. 1 c are the growing state scanning electron microscope (SEM) photograph on bacterial strain PM3 attachment sial system ceramic porous material；
Fig. 2 is that the Fourier before and after sial system ceramic porous material immobilization bacterial strain PM3 treatment simulated domestic wastewaters is infrared
Spectrogram.
Specific embodiment
Embodiment 1
Step 1：The BC soil of operational excellence and the sanitary sewage disposal subsurface wastewater infiltration system of stabilization is taken, it is dilute with sterilized water
Release, vibration dissolving stands, and obtains bacteria suspension two；
Step 2：Strain isolation is carried out using dilution plate rubbing method, bacteria suspension two is coated into ordinary solid culture medium
On, strain growth situation is observed, plate streaking purifying is carried out to single bacterium colony, 11 plants of different strains are obtained altogether；The ordinary solid
The chemical composition of culture medium is：Beef extract 5.0g, peptone 10.0g, sodium chloride 5.0g, agar 18g, distilled water 1L；
Step 3：The 11 plants of different strains obtained with step 2 live dirty with the simulation that 5% access amount puts into human configuration
In water, simulated domestic wastewater is processed, the OD600 according to bacterium solution draws the growth curve of bacterial strain, and bacterial strain PM3 is in 12h
Growth rate is most fast, to COD, NH in sewage4 +The clearance of-N and TP is respectively 99.8%, 63.9%, 22.2%, through 16S
RRNA identifies PM3 for trivalent arsenic oxidizing bacteria (i.e. Acidovorax sp.), and screening PM3 is high-efficient denitrification and dephosphorization bacterial strain；It is described
The chemical composition and concentration of simulated domestic wastewater be：COD：300mg/L, NH4 +-N：50mg/L, TP：20mg/L；
Step 4：It is to logarithmic phase, activation condition after activated strains PM3 activation 24h：29 DEG C of temperature, pH 7~9, shaking table turns
Fast 150r/min；The bacterial strain PM3 that OD600 values after 10mL is activated are 1 is taken with centrifuge tube, in centrifugation, centrifuge speed on centrifuge
9000r/min, centrifugation time 11min, are obtained the 10mL of bacteria suspension one；
Step 5：Take simulated domestic wastewater 200mL, carry out autoclaving, in an aseptic environment, by the 10mL of bacteria suspension one and
Sial system ceramic porous material 2g is added in the simulated domestic wastewater of 200mL；Sial system ceramic porous material particle diameter is 2mm；
Step 6：Control sample is added in 200mL's to be only inoculated with free bacterial strain PM3 with 5% access amount inoculating strain PM3
In simulated domestic wastewater；
Step 7：According to the growth curve of immobilization bacterial strain PM3 and free bacterial strain PM3, the growth of bacterial strain PM3 after immobilization
Speed and biological concentration are significantly improved, and immobilization bacterial strain PM3 is to COD, NH in simulated domestic wastewater4 +The clearance of-N and TP
Respectively 100%, 75.9%, 29.6%, specific ionization bacterial strain PM3 is to COD, NH in simulated domestic wastewater4 +The clearance of-N and TP
It is respectively increased 1.16%, 18.6%, 29.58%.
Embodiment 2
Step 1：The BC soil of operational excellence and the sanitary sewage disposal subsurface wastewater infiltration system of stabilization is taken, it is dilute with sterilized water
Release, vibration dissolving stands, and obtains bacteria suspension two；
Step 2：Strain isolation is carried out using dilution plate rubbing method, bacteria suspension two is coated into ordinary solid culture medium
On, strain growth situation is observed, plate streaking purifying is carried out to single bacterium colony, 11 plants of different strains are obtained altogether；The ordinary solid
The chemical composition of culture medium is：Beef extract 5.0g, peptone 10.0g, sodium chloride 5.0g, agar 18g, distilled water 1L；
Step 3：The 11 plants of different strains obtained with step 2 live dirty with the simulation that 5% access amount puts into human configuration
In water, simulated domestic wastewater is processed, OD600 according to bacterium solution draws the growth curve of bacterial strain, bacterial strain PM7 24h~
Growth rate is most fast during 48h, to COD, NH in sewage4 +The clearance of-N and TP is respectively 100%, 85.01%, 21.04%, warp
16S rRNA identify PM7 for spindle lysine bacillus (i.e. Lysinibacillus fusiformis), and screening PM7 is
High-efficient denitrification and dephosphorization bacterial strain；The chemical composition and concentration of the simulated domestic wastewater be：COD：300mg/L, NH4 +-N：50mg/L,
TP：20mg/L；
Step 4：It is to logarithmic phase, activation condition after activated strains PM7 activation 24h：29 DEG C of temperature, pH 7~9, shaking table turns
Fast 150r/min；The bacterial strain PM7 that OD600 values after 10mL is activated are 1 is taken with centrifuge tube, in centrifugation, centrifuge speed on centrifuge
9000r/min, centrifugation time 11min, are obtained bacteria suspension 10mL；
Step 5：Simulated domestic wastewater 200mL is taken, autoclaving is carried out, in an aseptic environment, by bacteria suspension in step 4
10mL and sial system ceramic porous material 2g are added in the simulated domestic wastewater of 200mL；Sial system ceramic porous material particle diameter
It is 2mm；
Step 6：Control sample is added in 200mL's to be only inoculated with free bacterial strain PM7 with 5% access amount inoculating strain PM7
In simulated domestic wastewater；
Step 7：Growth curves of the immobilization bacterial strain PM7 and free bacterial strain PM7 in sewage is simulated is drawn, bacterial strain is contrasted
To COD, NH in simulated domestic wastewater before and after PM7 immobilizations4 +The clearance and immobilization effect of-N and TP.
Embodiment 3
As shown in Fig. 1 a~1c, added into common with the bacterial strain PM3 and 2g sial system ceramic porous material of 5% access amount
In fluid nutrient medium, bacterial strain PM3 apposition growths are good；The chemical composition and concentration of the common liq culture medium be：Beef extract
5.0g, peptone 10.0g, sodium chloride 5.0g, distilled water 1L.
The cultivation temperature for setting immobilization bacterial strain PM3 is 30 DEG C, and in t=48h, immobilization bacterial strain PM3 lives to simulation
COD, NH in sewage4 +The clearance of-N and TP is respectively 99.996%, 92.38%, 45.28%.
Embodiment 4
In common liq culture medium being added with the bacterial strain PM3 and 2g sial system ceramic porous material of 5% access amount, bacterium
Strain PM3 apposition growths are good；The chemical composition and concentration of the common liq culture medium be：Beef extract 5.0g, peptone
10.0g, sodium chloride 5.0g, distilled water 1L.
The cultivation temperature for setting immobilization bacterial strain PM3 is 10 DEG C, and in t=6d, immobilization bacterial strain PM3 is dirty to simulation life
COD, NH in water4 +The clearance of-N and TP is respectively 99.59%, 70.81%, 38.96%.
Embodiment 5
In common liq culture medium being added with the bacterial strain PM7 and 4g sial system ceramic porous material of 5% access amount, bacterium
Strain PM7 apposition growths are good；The chemical composition and concentration of the common liq culture medium be：Beef extract 5.0g, peptone
10.0g, sodium chloride 5.0g, distilled water 1L.
The cultivation temperature for setting immobilization bacterial strain PM7 is 25 DEG C, and in t=60h, immobilization bacterial strain PM7 lives to simulation
COD, NH in sewage4 +The clearance of-N and TP.
Embodiment 6
In common liq culture medium being added with the bacterial strain PM7 and 4g sial system ceramic porous material of 8% access amount, bacterium
Strain PM7 apposition growths are good；The chemical composition and concentration of the common liq culture medium be：Beef extract 5.0g, peptone
10.0g, sodium chloride 5.0g, distilled water 1L.
The cultivation temperature for setting immobilization bacterial strain PM7 is 25 DEG C, and in t=60h, immobilization bacterial strain PM7 lives to simulation
COD, NH in sewage4 +The clearance of-N and TP.
From Figure 2 it can be seen that sial system ceramic porous material has certain aperture structure, and large specific surface area, and material
It is relatively light, for bacterial strain PM3 growths provide larger bond area and activity space, while being conducive to oxygen, nutriment etc.
Decomposed for bacterial strain PM3 into sial system ceramic porous material inside and utilized, while being also beneficial to the metabolite of bacterial strain PM3 generations
Outside discharge sial system ceramic porous material.Bacterial strain PM3 is fixed using sial system ceramic porous material, can keep bacterial strain PM3's
High concentration and high activity, so as to strengthen sanitary sewage disposal efficiency.
Claims (5)
1. the method for sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage, it is characterised in that by sial system pottery
Matter porous material and high-efficient denitrification and dephosphorization bacterial strain are added in sanitary sewage, are processed；The sial system ceramic porous material
Dosage be 1~5g, the high-efficient denitrification and dephosphorization bacterial strain access amount be OD600 values be 0.1~2 one 1~10mL of bacteria suspension；
The bacteria suspension one is after high-efficient denitrification and dephosphorization bacterial strain activates 24h, to be obtained in being centrifuged on centrifuge, centrifuge speed 8000~
10000r/min, 10~12min of centrifugation time；The high-efficient denitrification and dephosphorization bacterial strain activation condition is：28~30 DEG C of temperature, pH 7
~8,130~160r/min of shaking speed.
2. the method for sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage according to claim 1, its
It is characterised by, its mineral phase composition of the sial system ceramic porous material includes quartz, mullite and rutile；Its chemical composition
It is SiO2、Al2O3、Fe2O3、K2O and impurity, SiO2Account for 56.78wt%, Al2O3Account for 37.11wt%, K2O accounts for 2.78wt%, Fe2O3
1.58wt% is accounted for, impurity accounts for 1.75wt%；Its duct size is 30~1000 μm, and porosity is 30~90%, and particle diameter is 5-
10mm；Sial system porous material is synthesized by two kinds of natural clay CN-01 and CN-02；Quartz content is accounted in the CN-01
15.4%, potassium feldspar accounts for 1.3%, and muscovite accounts for 28.1%, and kaolinite accounts for 55.2%；Quartz content is accounted in the CN-02
10.4%, potassium feldspar accounts for 0.6%, and muscovite accounts for 16.9%, and kaolinite accounts for 72.1%；The CN-01 exists with CN-02 grain diameters
Below 200 mesh；SiO in the CN-012Account for 56.18wt%, Al2O326.80wt% is accounted for, bright alkali accounts for 8.70wt%, K2O is accounted for
3.39wt%, Fe2O32.63wt% is accounted for, impurity accounts for 0.3wt%；SiO in the CN-022Account for 52.42wt%, Al2O3Account for
31.98wt%, bright alkali accounts for 10.90wt%, Fe2O3Account for 1.36wt%, K2O accounts for 2.04wt%, and impurity accounts for 1.3wt%.
3. the method for sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage according to claim 2, its
It is characterised by, the sial system ceramic porous material particle diameter is 2mm~2.5mm, is obtained using the method manually beaten.
4. the method for sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage according to claim 1, its
It is characterised by, the high-efficient denitrification and dephosphorization bacterial strain includes that trivalent arsenic oxidizing bacteria, Burkholderia group, spindle rely
Propylhomoserin bacillus, bacillus pumilus group and/or pseudomonas.
5. the method for sial system ceramic porous material treatment based on immobilized microorganisms sanitary sewage according to claim 4, its
It is characterised by, the preparation process of the high-efficient denitrification and dephosphorization bacterial strain is as follows：
Step 1：The BC soil of operational excellence and the sanitary sewage disposal subsurface wastewater infiltration system of stabilization is taken, is diluted with sterilized water,
Vibration dissolving, stands, and obtains bacteria suspension two；
Step 2：Strain isolation is carried out using dilution plate rubbing method, bacteria suspension two is coated on ordinary solid culture medium, seen
Strain growth situation is examined, plate streaking purifying is carried out to single bacterium colony, to obtain many plants of different strains；The ordinary solid culture medium
Chemical composition and concentration be：Beef extract 5.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, 15.0~20.0g/L of agar,
Distilled water 1L/L；
Step 3：The many plants of different strains obtained with step 2 are lived with the simulation that 1%~10% access amount puts into human configuration
In sewage, simulated domestic wastewater is processed, investigate many plants of different strains to pollutant COD, NH4 +The removal effect of-N and TP
Really, after through screening, classified using 16S rRNA identification systems, obtained the high-efficient denitrification and dephosphorization bacterial strain；The simulation life
The chemical composition and concentration of sewage living be：COD：200~400mg/L, NH4 +-N：20~60mg/L, TP：10~30mg/L.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09220089A (en) * | 1996-02-19 | 1997-08-26 | Shinagawa Refract Co Ltd | Ceramic carrier for immobilizing microorganism |
| CN103880184A (en) * | 2014-03-27 | 2014-06-25 | 孔晔 | Sludge-free biologic ball ultra-clean sewage treatment method and system |
-
2017
- 2017-01-23 CN CN201710058901.XA patent/CN106745816A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09220089A (en) * | 1996-02-19 | 1997-08-26 | Shinagawa Refract Co Ltd | Ceramic carrier for immobilizing microorganism |
| CN103880184A (en) * | 2014-03-27 | 2014-06-25 | 孔晔 | Sludge-free biologic ball ultra-clean sewage treatment method and system |
Non-Patent Citations (1)
| Title |
|---|
| 王海燕等: "地下渗滤***处理农村生活污水优势菌株筛选及其脱氮除磷性", 《第十三届全国水处理化学大会暨海峡两岸水处理化学研讨会摘要集-S2生物法》 * |
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Application publication date: 20170531 |