CN106727923A - A kind of anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles and preparation method thereof - Google Patents

A kind of anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles and preparation method thereof Download PDF

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Publication number
CN106727923A
CN106727923A CN201710030415.7A CN201710030415A CN106727923A CN 106727923 A CN106727923 A CN 106727923A CN 201710030415 A CN201710030415 A CN 201710030415A CN 106727923 A CN106727923 A CN 106727923A
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silver particles
ammonium salt
quaternary ammonium
chitosan
bacteriostatic solution
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卢永平
卢冬丽
卢冬纯
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Puning Kangte Biotechnology Co Ltd
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Puning Kangte Biotechnology Co Ltd
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/534—Mentha (mint)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
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    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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    • A61K31/716—Glucans
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    • A—HUMAN NECESSITIES
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Abstract

A kind of anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles, the anti-bacteriostatic solution is by following components into being grouped into：Nano silver particles 5%~20%, Quaternary Ammonium Salt of Chitosan 0.5%~5%, ethanol 1%~3%, peppermint 0.1%~0.4%, remaining component is pure water.The beneficial effects of the invention are as follows, there is provided a kind of anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles and preparation method thereof, anti-antibacterial effect, antibiotic rate ＞ 99.99% can be effectively played.

Description

A kind of anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles and preparation method thereof
Technical field
The present invention relates to a kind of antiseptic solution, more particularly to a kind of anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles and its system Preparation Method.
Background technology
Be flooded with a large amount of bacteriums in life, silver be all since ancient times be verified with very strong antibacterial ability and have The bactericidal effect of wide spectrum, Chinese patent CN101223887B proposes a kind of nano-silver antiseptic solution and preparation method, the original for using Expect to be the nano silver particles and carboxymethyl chitosan of particle diameter 3nm~80nm, with antibacterial ability very high, its initial average antibacterial Rate reaches 99.96%, and one annual antibiotic rate of storage reaches 90.35%, and sterilizing rate is lower slightly.
The content of the invention
It is an object of the present invention to provide a kind of anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles, to solve the above problems.
Another object of the present invention is to, there is provided the preparation method of the above-mentioned anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles.
The present invention is accomplished by the following way：
A kind of anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles, the anti-bacteriostatic solution is by following components into being grouped into：Receive Rice silver particles 5%~20%, Quaternary Ammonium Salt of Chitosan 0.5%~5%, ethanol 1%~3%, peppermint 0.1%~0.4%, remaining group It is divided into pure water.
Further, the anti-bacteriostatic solution by following components into being grouped into：Nano silver particles 5%, Quaternary Ammonium Salt of Chitosan 1%, ethanol 2%, peppermint 0.1%, remaining component is pure water.
Further, a diameter of 2nm~20nm of the nano silver particles.
Further, the most preferred diameters of the nano silver particles are 2nm.
The preparation method of the above-mentioned anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles, comprises the following steps：
S1：Dissolved at normal temperatures during Quaternary Ammonium Salt of Chitosan is added into pure water, and stood 10~14 hours；
S2：Nano silver particles are added into the mixed solution prepared by S1, suspension is made；
S3：Suspension prepared by S2 is stirred, mixing time 30 minutes is uniformly distributed nano silver particles, shape Into the anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles.
Further, the S1 stages Quaternary Ammonium Salt of Chitosan adds the optimal standing dissolved at normal temperatures in pure water Time is 12 hours.
Core content of the invention is that Quaternary Ammonium Salt of Chitosan and silver particles are added in raw material, and Quaternary Ammonium Salt of Chitosan is with pure Water purification leaching is molten 12 hours, is completely dissolved into liquid, and silver particles are extracted its silver from freshwater mussel pearl, are made into nanometer technology Nanometer-level silver particle, add chitosan solution, the two mix after Cheng Kangyin solution.
Shitosan is lived as a kind of natural polymer with excellent biological functionality and compatibility with preferable antibacterial Property, the growth and breeding of some fungies, bacterium and virus can be suppressed, chitosan gel rubber can add silver as the carrier of silver particles Anti- bacteriostasis can be strengthened after particle.
According to the examining report at Guangzhou industrial microorganism center, the solution antibiotic rate ＞ made by above-mentioned preparation method 99.99%.
The beneficial effects of the invention are as follows, there is provided a kind of anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles and preparation method thereof, Anti- antibacterial effect, antibiotic rate ＞ 99.99% can be effectively played.
Specific embodiment
A kind of anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles and preparation method thereof：
Its silver is extracted from freshwater mussel pearl, the nanometer-level silver particle that particle diameter is 2nm is made into nanometer technology, then adopt Take following steps：
S1：Dissolved at normal temperatures during Quaternary Ammonium Salt of Chitosan is added into pure water, and stood 12 hours；
S2：Nano silver particles are added into the mixed solution prepared by S1, suspension is made；
S3：Suspension prepared by S2 is stirred, mixing time 30 minutes is uniformly distributed nano silver particles, shape Into the anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles.
According to the clinical data that concrete practice draws, during above-mentioned S1, in Quaternary Ammonium Salt of Chitosan addition pure water simultaneously Dissolved at normal temperatures, be within 12 hours optimal, then sterilizing rate can slowly be declined dissolution time more than 12 hours, sterilizing rate is in line Property downward trend.
Above-mentioned S2 and S3, adds nano silver particles and is stirred, and is in order that nano silver particles are uniformly distributed in dissolving In Quaternary Ammonium Salt of Chitosan afterwards so that the anti-bacteriostatic solution ratio uniform made.

Claims (6)

1. a kind of anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles, it is characterised in that：The anti-bacteriostatic solution by following components into It is grouped into：Nano silver particles 5%~20%, Quaternary Ammonium Salt of Chitosan 0.5%~5%, ethanol 1%~3%, peppermint 0.1%~ 0.4%, remaining component is pure water.
2. anti-bacteriostatic solution according to claim 1, it is characterised in that：The anti-bacteriostatic solution is by following components into packet Into：Nano silver particles 5%, Quaternary Ammonium Salt of Chitosan 1%, ethanol 2%, peppermint 0.1%, remaining component is pure water.
3. anti-bacteriostatic solution according to claim 1 and 2, it is characterised in that：A diameter of 2nm of the nano silver particles~ 20nm。
4. anti-bacteriostatic solution according to claim 3, it is characterised in that：The most preferred diameters of the nano silver particles are 2nm。
5. the preparation method of the anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles described in a kind of claim 1, it is characterised in that：Bag Include following steps：
S1：Dissolved at normal temperatures during Quaternary Ammonium Salt of Chitosan is added into pure water, and stood 10~14 hours；
S2：Nano silver particles are added into the mixed solution prepared by S1, suspension is made；
S3：Suspension prepared by S2 is stirred, mixing time 30 minutes is uniformly distributed nano silver particles, forms season The anti-bacteriostatic solution of ammonium salt shitosan silver particles.
6. preparation method according to claim 5, it is characterised in that：The S1 stages Quaternary Ammonium Salt of Chitosan adds pure water In the optimal time of repose that is dissolved at normal temperatures be 12 hours.
CN201710030415.7A 2017-01-17 2017-01-17 A kind of anti-bacteriostatic solution of Quaternary Ammonium Salt of Chitosan silver particles and preparation method thereof Pending CN106727923A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107019002A (en) * 2017-06-02 2017-08-08 兴化市恒威生物技术有限公司 Culture of Chinese mitten crab pool-cleaning agent and preparation method thereof
CN107258814A (en) * 2017-07-11 2017-10-20 广东火云生态科技有限公司 A kind of composite disinfectant of Quick disinfection sterilization and its preparation method and application
CN110215534A (en) * 2018-12-08 2019-09-10 普宁康特生物科技有限公司 A kind of nanometer level pearl silver is medical to resist antibacterial gauze
CN111995915A (en) * 2020-08-11 2020-11-27 深圳市鼎元恒泰科技有限公司 Water-soluble composite antibacterial coating and preparation method thereof
CN112704088A (en) * 2020-12-29 2021-04-27 南京启佑生物科技有限公司 Special disinfectant for chopping board and preparation method and application thereof
WO2021183041A1 (en) * 2020-03-09 2021-09-16 Kit Yew Cheng The chemical composition for impregnation via spraying on disposable sugical masks, sleeves gowns made from hydrophilic and hydrophobic materials for the inactivation of bacteria and viruses such as coronavirus, sars, mers, influenza a and influenza b viruses
CN114097825A (en) * 2021-12-16 2022-03-01 山东大学 Quaternized chitosan coated Ag-loaded CoP nanoneedle and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102228995A (en) * 2011-06-30 2011-11-02 华南理工大学 Polysaccharide-nano silver sol and preparation method thereof
CN103834050A (en) * 2014-03-21 2014-06-04 北京科技大学 Method for preparing gelatin/nano-silver/chitosan derivative composite film
CN104232333A (en) * 2014-08-27 2014-12-24 王晓伟 Nano-silver inorganic antibacterial detergent cream
KR20160044887A (en) * 2014-10-16 2016-04-26 (주)비케이 Adsorbent using waterworks sludge and manufacturing method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102228995A (en) * 2011-06-30 2011-11-02 华南理工大学 Polysaccharide-nano silver sol and preparation method thereof
CN103834050A (en) * 2014-03-21 2014-06-04 北京科技大学 Method for preparing gelatin/nano-silver/chitosan derivative composite film
CN104232333A (en) * 2014-08-27 2014-12-24 王晓伟 Nano-silver inorganic antibacterial detergent cream
KR20160044887A (en) * 2014-10-16 2016-04-26 (주)비케이 Adsorbent using waterworks sludge and manufacturing method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
来水利 等: "纳米Ag/HACC复合抗菌整理", 《印染》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107019002A (en) * 2017-06-02 2017-08-08 兴化市恒威生物技术有限公司 Culture of Chinese mitten crab pool-cleaning agent and preparation method thereof
CN107258814A (en) * 2017-07-11 2017-10-20 广东火云生态科技有限公司 A kind of composite disinfectant of Quick disinfection sterilization and its preparation method and application
CN110215534A (en) * 2018-12-08 2019-09-10 普宁康特生物科技有限公司 A kind of nanometer level pearl silver is medical to resist antibacterial gauze
WO2021183041A1 (en) * 2020-03-09 2021-09-16 Kit Yew Cheng The chemical composition for impregnation via spraying on disposable sugical masks, sleeves gowns made from hydrophilic and hydrophobic materials for the inactivation of bacteria and viruses such as coronavirus, sars, mers, influenza a and influenza b viruses
CN111995915A (en) * 2020-08-11 2020-11-27 深圳市鼎元恒泰科技有限公司 Water-soluble composite antibacterial coating and preparation method thereof
CN112704088A (en) * 2020-12-29 2021-04-27 南京启佑生物科技有限公司 Special disinfectant for chopping board and preparation method and application thereof
CN114097825A (en) * 2021-12-16 2022-03-01 山东大学 Quaternized chitosan coated Ag-loaded CoP nanoneedle and preparation method thereof

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