CN106721663A - A kind of method that bioanalysis prepares cobio expanding compound feed using glasswort - Google Patents
A kind of method that bioanalysis prepares cobio expanding compound feed using glasswort Download PDFInfo
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- CN106721663A CN106721663A CN201611239595.1A CN201611239595A CN106721663A CN 106721663 A CN106721663 A CN 106721663A CN 201611239595 A CN201611239595 A CN 201611239595A CN 106721663 A CN106721663 A CN 106721663A
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- 238000000034 method Methods 0.000 title claims abstract description 17
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- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses a kind of method that bioanalysis prepares cobio expanding compound feed using glasswort, the drawbacks of both overcome existing feed fish meal consumption high, turning waste into wealth again high-valued has developed glasswort resource.The feed product is mainly and is made up of raw materials such as glasswort yeast powder, steam fish meal, fermented bean dregs, double lower rapeseed dreg, DDGS, flour, sodium alginate, ocean fish oil, maize germ oil, soybean lecithin, cuttlefish cream, Choline Chloride, glycine betaine, inositol, B B-complex, composite trace element, allicin, bacillus licheniformis, bifidobacterium bifidum, phytase, non-starch polysaccharide enzymes, and the glasswort yeast powder is obtained by raw materials such as 30 parts of yam flour, the Radix Astragali, Radix Glycyrrhizae, rhizoma zingiberis, malt, wheat bran, STEVIA REBAUDIANAs by two-step fermentation technique.Product has and remarkably promotes cobio rapid growth, improves cultivation survival rate, reduces aquaculture cost, improves cultivation digestibility, reduces nitrogen, phosphorus excretion, the advantages of improve breeding environment, with cost performance and application value higher.
Description
Technical field
The present invention relates to a kind of method that bioanalysis prepares cobio expanding compound feed using glasswort, belong to aquatic products feeding
Material manufacture field.
Background technology
Glasswort belongs to jasmine section plant, glasswort category, in salt life also known as extra large asparagus, extra large pilose antler, marine borer grass, rich and honour dish etc.
In plant classification, belong to stem carnification euhalophyte, be more common in marshland, salt lake side and and seabeach.Glasswort its dry plant
Strain containing cellulose 5~20%, protein 9.0%, rich in 8 kinds of amino acid needed by human, trace element and vitamin, wherein recklessly
Radish cellulose content exceedes 40 times of common vegetable, also containing natural plant health salt and natural vegetable soda (micro- angle saponin(e), tool
There is the effects such as reduction cholesterol, step-down.The plantation of glasswort has significant application value to improving China's saline-alkali environment, but at present
The comprehensive development and utilization degree of glasswort is relatively low, governs glasswort secondary industry economic benefit, also directly affects glasswort
Plantation promote, therefore, if glasswort is carried out into high-valued comprehensive development and utilization, important tribute will be made to environment and economy
Offer.
Cobio (Rachycentron canadum) category Perciformes, cobio section, cobio category, are commonly called as Cobia, sea otter
The carnivorous straddling fish stocks of fish, the torrid zone and subtropical seas, are distributed mainly on the Atlantic Ocean, the Indian Ocean and (Eastern Pacific, the Pacific Ocean
Except) etc. littoral sea and bay, China is also distributed.The cobio resistance against diseases of cage culture is strong, and growth is fast, individual big,
Fine and tender taste, is one of important species of current sea-farming.In recent years, with the success of seed and larvae cultivation, cobio
Cultivation scale in countries such as the coastal all provinces (especially Guangdong, Hainan) of south China, Southeast Asia is increasing.But mesh
Existing Feed of Rachycentron canadum in preceding China market, is simply combined with single raw material or several raw materials, and nutrition is not comprehensive enough, feed
Utilization rate is low, it is impossible to meet the nutritional need of cobio growth, and fanning economics are low, as limitation cobio aquaculture industry
The bottleneck of sustainable development, exploitation cobio scrod swelling mixed feed efficiently, environmentally friendly turns into and promotes cobio aquaculture industry
The matter of utmost importance of development.
Additionally, in current Feed of Rachycentron canadum, typically using fish meal as main protein source.In recent years by the whole world
The influence of the fishery decline of natural resources, world's fish meal yield declines year by year, and the fast development of culture fishery so that fish meal
Consumption figure is increasingly increased.At present because the shortage phenomenon of fish meal causes fish meal prices rapidly to go up, if do not taken measures as early as possible, generation
Powerless support culture fishery is kept the development of fair speed by boundary's fish meal production, therefore seeks to substitute the approach of fish meal as dynamic
Thing nutrient research field and feed manufacturing emterprise put forth effort one of hot issue for solving.Current cobio high-quality feed abroad
In, the animal protein content such as fish meal, shrimp med feeds this kind of feed and is clearly present two problems more than 40%, one be feed into
This is higher, and fanning economics are relatively low；Two be due to the phosphorus containing higher amount in animal protein, it is substantial amounts of not by fish body absorb profit
Phosphorus is discharged into environment, causes the concentration of breeding water body phosphorus significantly to raise, and with the extension of culturing time, phosphorus is in water body
Constantly accumulation, certainly will cause the generation of body eutrophication.Therefore, try to explore to substitute fish meal using suitable protein sources,
Employ new technology, new technology, exploitation, development of new environment-friendly high-efficiency feed formula, to reduce feed cost, reduce environmental pollution,
Improve cobio culturing economic, a kind of effective way of ecological benefits.
The content of the invention
It is it is an object of the invention to provide a kind of recycling glasswort and high etc. with comprehensive nutrition, nutrition digestibility
The cobio expanding compound feed of advantage, had both overcome the problem of existing Feed of Rachycentron canadum not comprehensive nutrition, reduced to water environment
Pollution problem, turn waste into wealth again it is high-valued developed glasswort resource, reduce dependence of the cobio aquaculture to fish meal.
The method that a kind of bioanalysis of the present invention prepares cobio expanding compound feed using glasswort, its feature exists
Then it is made up of the raw material of following weight portion：10~20 parts of glasswort yeast powder, 15~20 parts of steam fish meal, fermented bean dregs 20~
25 parts, 10~15 parts of double lower rapeseed dreg, DDGS4~7 part, 15~20 parts of flour, 6~8 parts of sodium alginate, 3~5 parts of ocean fish oil,
2~4 parts of maize germ oil, 1~2 part of soybean lecithin, 1~3 part of cuttlefish cream, 0.5~1.5 part of Choline Chloride, glycine betaine 0.2
Part, 0.2 part of inositol, 0.5 part of B B-complex, 1 part of composite trace element, 0.5 part of allicin, 10,000,000,000/gram bacillus licheniformis
0.1 part, 0.1 part of 10,000,000,000/gram bifidobacterium bifidum, 0.1 part of 5000U/g phytases, 0.1 part of 8000U/g non-starch polysaccharide enzymes.
The method that a kind of above-mentioned bioanalysis prepares cobio expanding compound feed using glasswort, it is characterised in that preferably
Scheme is made of the raw material of following weight portion：15 parts of glasswort yeast powder, 18 parts of steam fish meal, 22 parts of fermented bean dregs, double low dishes
12 parts of the seed dregs of rice, DDGS6 parts, 18 parts of flour, 7 parts of sodium alginate, ocean fish oil 4 parts, 3 parts of maize germ oil, soybean lecithin 1.5
Part, 2 parts of cuttlefish cream, 1 part of Choline Chloride, 0.2 part of glycine betaine, 0.2 part of inositol, 0.5 part of B B-complex, composite trace element 1
Part, 0.5 part of allicin, 0.1 part of 10,000,000,000/gram bacillus licheniformis, 0.1 part of 10,000,000,000/gram bifidobacterium bifidum, 5000U/g plant
0.1 part of sour enzyme, 0.1 part of 8000U/g non-starch polysaccharide enzymes.
Above-mentioned glasswort yeast powder is made by the steps in the present invention：
(1) immersion is crushed：Deimpurity glasswort cane and herbal raw material in mass ratio 95 will be removed:5 mixing, after crushing
100 mesh sieves are crossed, 2 times of quality is subsequently adding and is soaked 3~5h than pure water；
(2) boiling：By the glasswort suspension 1~2h of boiling after immersion, then decline in air-proof condition and warm to room temperature；
(3) separate：Cooking liquor is carried out into separation of solid and liquid, glasswort cane powder is obtained, the water after separation is carried out into step (1)
Recycle；
(4) one-step fermentation：The salt tolerant Alkaliphilic bacillus of inoculation glasswort mass ratio 3%, issue in 28~35 DEG C of conditions
2~3d of ferment；
(5) two-step fermentation：After one-step fermentation terminates, the cellulase and 1% non-starch of glasswort mass ratio 2% are added
Polysaccharase, and be inoculated with the candida utili of glasswort mass ratio 2% and 1% aspergillus niger, ferments 3 under the conditions of 28~35 DEG C
~5d, stirring in 12 hours is once；Glasswort yeast powder is obtained final product after fermentation ends.
Gained glasswort yeast powder protein content >=20%.
Said herbal medicine raw material is made up of the Chinese herbal medicine of following mass ratio in the present invention：30 parts of yam flour, 20 parts of the Radix Astragali,
20 parts of Radix Glycyrrhizae, 10 parts of rhizoma zingiberis, 10 parts of malt, 10 parts of wheat bran, 10 parts of STEVIA REBAUDIANA.
B B-complex and composite trace element in heretofore described raw material are commercially available cobio special feed and add
Plus agent.
The present invention provides a kind of production technology for adding glasswort preparation cobio expanding compound feed, it is characterised in that army
Cao's fish expanding compound feed is achieved through the following channels：
(1) raw material proportioning：Each raw material components are matched according to aforementioned proportion；
(2) pretreatment of raw material：Glasswort yeast powder, fermented bean dregs, double lower rapeseed dreg, flour, DDGS are mixed, adjustment is former
Material water content is 10~15%, and the lactic acid bacteria of inoculation 3~5% stirs, the anaerobic fermentation 3~5 under the conditions of 25~35 DEG C
My god；
(3) mix：By pretreated raw material and steam fish meal, sodium alginate, soybean lecithin, cuttlefish cream, chlorination courage
Alkali, glycine betaine, inositol, composite trace element, allicin are well mixed, and mixture homogeneity is more than 95%；
(4) ultramicro grinding：Ultramicro grinding after well mixed, the screen cloth of 20~40 mesh can be passed through to each raw material；
(5) it is quenched：Material after crushing is passed through 90~95 DEG C of quenched 5~8 points of vapor in Double-shaft differential modulator
Clock；
(6) it is expanded：Ocean fish oil, maize germ oil are added, raw material is entered extruding at a temperature of 90~98 DEG C swollen
The expanded granulation of change machine；
(7) add afterwards：The mode of addition adds B B-complex, bacillus licheniformis, bifidobacterium bifidum, phytic acid later
Enzyme, non-starch polysaccharide enzyme, are well mixed；
(8) dry：At a temperature of 93~100 DEG C, by the drying of obtained particle；
(9) sieve：According to the particle demand in different raising periods, select the sieves of different size to be sieved, prepare
Into cobio expanding compound feed.
The cobio expanding compound feed that a kind of bioanalysis of present invention offer is prepared using glasswort, the product crude protein >=
40%, crude fat >=8%, coarse ash≤5%.This product is researched and developed according to the physilogical characteristics of cobio, is belonged to curing fermented type and is raised
Material, is particularly suitable for cobio feed, enters water and absorbs moisture, softens, and is conducive to digesting and assimilating for fish, it is ensured that fish enteron aisle is good for
Health；With feed coefficient is low, good palatability, water stability are strong, can significantly improve the immunity and abilities of digestive and absorption of fish body
Etc. advantage.
The present invention is directed to cobio digestive physiological characteristics, the high level recycling based on glasswort, by feed formula
Optimization, fish meal content was too high in both having solved the problems, such as expanded pellet diet, improve the influence digestibility and palatability of feed, and
Realize the high-valued comprehensive utilization of glasswort.The invention feed is cultivated into cobio rapid growth, raising is remarkably promoted
Motility rate, reduce aquaculture cost, improve cultivation digestibility, reduce nitrogen, phosphorus excretion, improve breeding environment the advantages of, with compared with
Cost performance high.
The present invention provides the cobio expanding compound feed that a kind of bioanalysis is prepared using glasswort, it is advantageous that：One
That glasswort is with the addition of in cobio expanding compound feed of the present invention, glasswort rich in carbohydrate, protein, crude fibre,
The nutriments such as mineral matter, fat and vitamin, improve the trophism and feature of feed, by between glasswort and Chinese herbal medicine
Synergy be obviously improved feed nutritive value and cobio culture benefit, and without additionally add salt in feed；Two are
The usage amount of fish meal is reduced by adding glasswort, fermented bean dregs, rapeseed dregs, flour etc., is conducive to breaking fish meal scarcity right
The constraint of China's aquatic products industry, reduces pollution of the aquaculture to environment, promotes culture fishery sustainable development；Three is cobio
Be with the addition of in expanding compound feed complex microorganism, composite trace element, phytase, non-starch polysaccharide enzyme, bacillus licheniformis,
Bacillus acidi lactici etc., improves the palatability and nutrition digestibility of product, reduces feed coefficient, improves cobio immunity of organism
Power, reduces cultivation risk.
Specific embodiment
Embodiment 1：
A kind of cobio expanding compound feed, specific preparation process is as follows：
(1) herbal raw material prepares：By 30 parts of yam flour, 20 parts of the Radix Astragali, 20 parts of Radix Glycyrrhizae, 10 parts of rhizoma zingiberis, 10 parts of malt, bran
10 parts of precise Chinese herbal medicines of skin, go the removal of impurity；
(2) immersion is crushed：Deimpurity glasswort cane and herbal raw material in mass ratio 95 will be removed:5 mixing, after crushing
100 mesh sieves are crossed, 2 times of quality is subsequently adding and is soaked 3~5h than pure water；
(3) boiling：By the glasswort suspension 1~2h of boiling after immersion, then decline in air-proof condition and warm to room temperature；
(4) separate：Cooking liquor is carried out into separation of solid and liquid, glasswort cane powder is obtained, the water after separation is carried out into step (1)
Recycle；
(5) one-step fermentation：The salt tolerant Alkaliphilic bacillus of inoculation glasswort mass ratio 3%, issue in 28~35 DEG C of conditions
2~3d of ferment；
(6) two-step fermentation：After one-step fermentation terminates, the cellulase and 1% non-starch of glasswort mass ratio 2% are added
Polysaccharase, and be inoculated with the candida utili of glasswort mass ratio 2% and 1% aspergillus niger, ferments 3 under the conditions of 28~35 DEG C
~5d, stirring in 12 hours is once；Glasswort yeast powder is obtained final product after fermentation ends；
(7) feedstuff proportioning：According to 15 parts of glasswort yeast powder, 18 parts of steam fish meal, 22 parts of fermented bean dregs, double low dishes
12 parts of the seed dregs of rice, DDGS6 parts, 18 parts of flour, 7 parts of sodium alginate, ocean fish oil 4 parts, 3 parts of maize germ oil, soybean lecithin 1.5
Part, 2 parts of cuttlefish cream, 1 part of Choline Chloride, 0.2 part of glycine betaine, 0.2 part of inositol, 0.5 part of B B-complex, composite trace element 1
Part, 0.5 part of allicin, 0.1 part of 10,000,000,000/gram bacillus licheniformis, 0.1 part of 10,000,000,000/gram bifidobacterium bifidum, 5000U/g plant
0.1 part of sour enzyme, 0.1 part of ratio of 8000U/g non-starch polysaccharide enzymes accurately match each raw material components；
(8) pretreatment of raw material：Glasswort yeast powder, fermented bean dregs, double lower rapeseed dreg, flour, DDGS are mixed, adjustment is former
Material water content is 10~15%, and the lactic acid bacteria of inoculation 3~5% stirs, the anaerobic fermentation 3~5 under the conditions of 25~35 DEG C
My god；
(9) mix：By pretreated raw material and steam fish meal, sodium alginate, soybean lecithin, cuttlefish cream, chlorination courage
Alkali, glycine betaine, inositol, composite trace element, allicin are well mixed, and mixture homogeneity is more than 95%；
(10) ultramicro grinding：Ultramicro grinding after well mixed, the screen cloth of 20~40 mesh can be passed through to each raw material；
(11) it is quenched：Material after crushing is passed through 90~95 DEG C of quenched 5~8 points of vapor in Double-shaft differential modulator
Clock；
(12) it is expanded：Ocean fish oil, maize germ oil are added, raw material is entered extruding at a temperature of 90~98 DEG C swollen
The expanded granulation of change machine；
(13) add afterwards：The mode of addition adds B B-complex, bacillus licheniformis, bifidobacterium bifidum, plant later
Sour enzyme, non-starch polysaccharide enzyme, are well mixed；
(14) dry：At a temperature of 93~100 DEG C, by the drying of obtained particle；
(15) sieve：According to the particle demand in different raising periods, select the sieves of different size to be sieved, prepare
Into cobio expanding compound feed.
Embodiment 2：
A kind of cobio expanding compound feed, specific preparation process is as follows：
(1) herbal raw material prepares：By 30 parts of yam flour, 20 parts of the Radix Astragali, 20 parts of Radix Glycyrrhizae, 10 parts of rhizoma zingiberis, 10 parts of malt, bran
10 parts of precise Chinese herbal medicines of skin, go the removal of impurity；
(2) immersion is crushed：Deimpurity glasswort cane and herbal raw material in mass ratio 95 will be removed:5 mixing, after crushing
100 mesh sieves are crossed, 2 times of quality is subsequently adding and is soaked 3~5h than pure water；
(3) boiling：By the glasswort suspension 1~2h of boiling after immersion, then decline in air-proof condition and warm to room temperature；
(4) separate：Cooking liquor is carried out into separation of solid and liquid, glasswort cane powder is obtained, the water after separation is carried out into step (1)
Recycle；
(5) one-step fermentation：The salt tolerant Alkaliphilic bacillus of inoculation glasswort mass ratio 3%, issue in 28~35 DEG C of conditions
2~3d of ferment；
(6) two-step fermentation：After one-step fermentation terminates, the cellulase and 1% non-starch of glasswort mass ratio 2% are added
Polysaccharase, and the candida utili of glasswort mass ratio 2% is inoculated with, ferment 3~5d under the conditions of 28~35 DEG C, stirs within 12 hours
Mix once；Glasswort yeast powder is obtained final product after fermentation ends；
(7) feedstuff proportioning：According to 10 parts of glasswort yeast powder, 20 parts of steam fish meal, 20 parts of fermented bean dregs, double low dishes
15 parts of the seed dregs of rice, DDGS4 parts, 20 parts of flour, 6 parts of sodium alginate, ocean fish oil 5 parts, 2 parts of maize germ oil, 2 parts of soybean lecithin,
1 part of cuttlefish cream, 1.5 parts of Choline Chloride, 0.2 part of glycine betaine, 0.2 part of inositol, 0.5 part of B B-complex, composite trace element 1
Part, 0.5 part of allicin, 0.1 part of 10,000,000,000/gram bacillus licheniformis, 0.1 part of 10,000,000,000/gram bifidobacterium bifidum, 5000U/g plant
0.1 part of sour enzyme, 0.1 part of ratio of 8000U/g non-starch polysaccharide enzymes accurately match each raw material components；
(8) pretreatment of raw material：Glasswort yeast powder, fermented bean dregs, double lower rapeseed dreg, flour, DDGS are mixed, adjustment is former
Material water content is 10~15%, and the lactic acid bacteria of inoculation 3~5% stirs, the anaerobic fermentation 3~5 under the conditions of 25~35 DEG C
My god；
(9) mix：By pretreated raw material and steam fish meal, sodium alginate, soybean lecithin, cuttlefish cream, chlorination courage
Alkali, glycine betaine, inositol, composite trace element, allicin are well mixed, and mixture homogeneity is more than 95%；
(10) ultramicro grinding：Ultramicro grinding after well mixed, the screen cloth of 20~40 mesh can be passed through to each raw material；
(11) it is quenched：Material after crushing is passed through 90~95 DEG C of quenched 5~8 points of vapor in Double-shaft differential modulator
Clock；
(12) it is expanded：Ocean fish oil, maize germ oil are added, raw material is entered extruding at a temperature of 90~98 DEG C swollen
The expanded granulation of change machine；
(13) add afterwards：The mode of addition adds B B-complex, bacillus licheniformis, bifidobacterium bifidum, plant later
Sour enzyme, non-starch polysaccharide enzyme, are well mixed；
(14) dry：At a temperature of 93~100 DEG C, by the drying of obtained particle；
(15) sieve：According to the particle demand in different raising periods, select the sieves of different size to be sieved, prepare
Into cobio expanding compound feed.
Embodiment 3：
A kind of cobio expanding compound feed, specific preparation process is as follows：
(1) herbal raw material prepares：By 30 parts of yam flour, 20 parts of the Radix Astragali, 20 parts of Radix Glycyrrhizae, 10 parts of rhizoma zingiberis, 10 parts of malt, bran
10 parts of precise Chinese herbal medicines of skin, go the removal of impurity；
(2) immersion is crushed：Deimpurity glasswort cane and herbal raw material in mass ratio 95 will be removed:5 mixing, after crushing
100 mesh sieves are crossed, 2 times of quality is subsequently adding and is soaked 3~5h than pure water；
(3) boiling：By the glasswort suspension 1~2h of boiling after immersion, then decline in air-proof condition and warm to room temperature；
(4) separate：Cooking liquor is carried out into separation of solid and liquid, glasswort cane powder is obtained, the water after separation is carried out into step (1)
Recycle；
(5) one-step fermentation：The salt tolerant Alkaliphilic bacillus of inoculation glasswort mass ratio 3%, issue in 28~35 DEG C of conditions
2~3d of ferment；
(6) two-step fermentation：After one-step fermentation terminates, the cellulase and 1% non-starch of glasswort mass ratio 2% are added
Polysaccharase, and the candida utili of glasswort mass ratio 2% is inoculated with, ferment 3~5d under the conditions of 28~35 DEG C, stirs within 12 hours
Mix once；Glasswort yeast powder is obtained final product after fermentation ends；
(7) feedstuff proportioning：According to 20 parts of glasswort yeast powder, 15 parts of steam fish meal, 25 parts of fermented bean dregs, double low dishes
10 parts of the seed dregs of rice, DDGS7 parts, 15 parts of flour, 8 parts of sodium alginate, ocean fish oil 3 parts, 4 parts of maize germ oil, 1 part of soybean lecithin,
3 parts of cuttlefish cream, 1.5 parts of Choline Chloride, 0.2 part of glycine betaine, 0.2 part of inositol, 0.5 part of B B-complex, composite trace element 1
Part, 0.5 part of allicin, 0.1 part of 10,000,000,000/gram bacillus licheniformis, 0.1 part of 10,000,000,000/gram bifidobacterium bifidum, 5000U/g plant
0.1 part of sour enzyme, 0.1 part of ratio of 8000U/g non-starch polysaccharide enzymes accurately match each raw material components；
(8) pretreatment of raw material：Glasswort yeast powder, fermented bean dregs, double lower rapeseed dreg, flour, DDGS are mixed, adjustment is former
Material water content is 10~15%, and the lactic acid bacteria of inoculation 3~5% stirs, the anaerobic fermentation 3~5 under the conditions of 25~35 DEG C
My god；
(9) mix：By pretreated raw material and steam fish meal, sodium alginate, soybean lecithin, cuttlefish cream, chlorination courage
Alkali, glycine betaine, inositol, composite trace element, allicin are well mixed, and mixture homogeneity is more than 95%；
(10) ultramicro grinding：Ultramicro grinding after well mixed, the screen cloth of 20~40 mesh can be passed through to each raw material；
(11) it is quenched：Material after crushing is passed through 90~95 DEG C of quenched 5~8 points of vapor in Double-shaft differential modulator
Clock；
(12) it is expanded：Ocean fish oil, maize germ oil are added, raw material is entered extruding at a temperature of 90~98 DEG C swollen
The expanded granulation of change machine；
(13) add afterwards：The mode of addition adds B B-complex, bacillus licheniformis, bifidobacterium bifidum, plant later
Sour enzyme, non-starch polysaccharide enzyme, are well mixed；
(14) dry：At a temperature of 93~100 DEG C, by the drying of obtained particle；
(15) sieve：According to the particle demand in different raising periods, select the sieves of different size to be sieved, prepare
Into cobio expanding compound feed.
Young age cobio is chosen as subjects, using grazing rate method and maze method as test method, compares the hair
Bright product and two kinds of feeding effects of commercially available Cabio compounding feedstuff；Every group of initial cultivation amount 100, Feeding time 1 day 2 times,
It is at 9 points in the morning, each during afternoon 16 once manually to feed, feed duration 20min, test period 50d, each group remaining aquaculture management phase
Together.Experiment is grouped as follows：
Table 1 tests grouping sheet
The result of the test of table 2
In sum, the application achieves significant technique effect, although products material composition fish meal content is significantly reduced,
But cobio immunity and growth ability are improved and feed applications value has significant facilitation；And result of the test shows
Change glasswort fermentation powder raw material composition and (P is made a significant impact on product efficacy<0.05).
Above example is merely to illustrate technical scheme, rather than is limited；Although with reference to foregoing reality
Example is applied to being described in detail by invention, but for the person of ordinary skill of the art, still can be to foregoing reality
Apply the technical scheme described in example to modify, or equivalent is carried out to which part technical characteristic；And to these modifications
Or replace, do not make the spirit and scope of the essence disengaging claimed technical solution of the invention of appropriate technical solution.
Claims (5)
1. a kind of method that bioanalysis prepares cobio expanding compound feed using glasswort, it is characterised in that be by following weight
The raw material of part is made：10~20 parts of glasswort yeast powder, 15~20 parts of steam fish meal, 20~25 parts of fermented bean dregs, double low vegetable seed
10~15 parts of the dregs of rice, DDGS4~7 part, 15~20 parts of flour, 6~8 parts of sodium alginate, 3~5 parts of ocean fish oil, maize germ oil 2~4
It is part, 1~2 part of soybean lecithin, 1~3 part of cuttlefish cream, 0.5~1.5 part of Choline Chloride, 0.2 part of glycine betaine, 0.2 part of inositol, multiple
Close 0.5 part of vitamin, 1 part of composite trace element, 0.5 part of allicin 0.1 part of 10,000,000,000/gram bacillus licheniformis, 10,000,000,000/gram
0.1 part of bifidobacterium bifidum, 0.1 part of 5000U/g phytases, 0.1 part of 8000U/g non-starch polysaccharide enzymes.
2. the method that a kind of bioanalysis according to claim 1 prepares cobio expanding compound feed using glasswort, its
It is characterised by that the preferred scheme for preparing feed method is made of the raw material of following weight portion：15 parts of glasswort yeast powder, steaming
18 parts of vapour fish meal, 22 parts of fermented bean dregs, 12 parts of double lower rapeseed dreg, DDGS6 parts, 18 parts of flour, 7 parts of sodium alginate, ocean fish oil 4
Part, 3 parts of maize germ oil, 1.5 parts of soybean lecithin, 2 parts of cuttlefish cream, 1 part of Choline Chloride, 0.2 part of glycine betaine, 0.2 part of inositol,
0.5 part of B B-complex, 1 part of composite trace element, 0.5 part of allicin 0.1 part of 10,000,000,000/gram bacillus licheniformis, 10,000,000,000/
Gram 0.1 part of bifidobacterium bifidum, 0.1 part of 5000U/g phytases, 0.1 part of 8000U/g non-starch polysaccharide enzymes.
3. the method that a kind of bioanalysis according to claim 1 prepares cobio expanding compound feed using glasswort, its
It is characterised by that the glasswort yeast powder is made by the steps：
（1）Crush immersion：Deimpurity glasswort cane and herbal raw material in mass ratio 95 will be removed:5 mixing, mistake after crushing
100 mesh sieves, are subsequently adding 2 times of quality and soak 3~5h than pure water；
（2）Boiling：By the glasswort suspension 1~2h of boiling after immersion, then decline in air-proof condition and warm to room temperature；
（3）Separate：Cooking liquor is carried out into separation of solid and liquid, glasswort cane powder is obtained, the water after separation enters step（1）It is circulated
Utilize；
（4）One-step fermentation：The salt tolerant Alkaliphilic bacillus of inoculation glasswort mass ratio 3%, ferment 2 under the conditions of 28~35 DEG C~
3d；
（5）Two-step fermentation：After one-step fermentation terminates, the cellulase and 1% SNSP of glasswort mass ratio 2% are added
Enzyme, and the candida utili of glasswort mass ratio 2% and 1% aspergillus niger are inoculated with, ferment 3~5d under the conditions of 28~35 DEG C,
Stirring in 12 hours is once；Glasswort yeast powder is obtained final product after fermentation ends.
4. the method that a kind of bioanalysis according to claim 3 prepares cobio expanding compound feed using glasswort, its
It is characterised by that the herbal raw material is made up of the Chinese herbal medicine of following mass ratio：30 parts of yam flour, 20 parts of the Radix Astragali, Radix Glycyrrhizae 20
Part, 10 parts of rhizoma zingiberis, 10 parts of malt, 10 parts of wheat bran, 10 parts of STEVIA REBAUDIANA.
5. the method that a kind of bioanalysis according to claim 1 prepares cobio expanding compound feed using glasswort, its
It is characterised by that methods described specifically includes following steps：
（1）Raw material proportioning：Proportionally match each raw material components；
（2）Pretreatment of raw material：Glasswort yeast powder, fermented bean dregs, double lower rapeseed dreg, flour, DDGS are mixed, adjustment raw material contains
Water is 10~15%, and the lactic acid bacteria of inoculation 3~5% stirs, anaerobic fermentation 3~5 days under the conditions of 25~35 DEG C；
（3）Mixing：By pretreated raw material and steam fish meal, sodium alginate, soybean lecithin, cuttlefish cream, Choline Chloride, sweet
Dish alkali, inositol, composite trace element, allicin are well mixed, and mixture homogeneity is more than 95%；
（4）Ultramicro grinding：Ultramicro grinding after well mixed, the screen cloth of 20~40 mesh can be passed through to each raw material；
（5）It is quenched：Material after crushing is passed through 90~95 DEG C of vapor quenched 5~8 minutes in Double-shaft differential modulator；
（6）It is expanded：Ocean fish oil, maize germ oil are added, raw material is entered extruder at a temperature of 90~98 DEG C
Expanded granulation；
（7）After add：Later addition mode add B B-complex, bacillus licheniformis, bifidobacterium bifidum, phytase,
Non-starch polysaccharide enzyme, is well mixed；
（8）Drying：At a temperature of 93~100 DEG C, by the drying of obtained particle；
（9）Screening：According to the particle demand in different raising periods, the sieves of different size is selected to be sieved, preparation is established an army
Cao's fish expanding compound feed.
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Cited By (1)
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